Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52644.t1 | XP_003625240.2 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 2.60E-64 | 254.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52644.t1 | G7KVB6 | 98.5 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 1.9e-64 | 254.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene52644 | 0.833377 | 5.52E-56 | -1.69E-46 |
| MS.gene049825 | MS.gene52644 | 0.853005 | 3.18E-61 | -1.69E-46 |
| MS.gene049873 | MS.gene52644 | 0.809483 | 1.85E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene52644 | 0.809949 | 1.47E-50 | -1.69E-46 |
| MS.gene051687 | MS.gene52644 | 0.82787 | 1.23E-54 | -1.69E-46 |
| MS.gene051700 | MS.gene52644 | 0.847887 | 8.70E-60 | -1.69E-46 |
| MS.gene055465 | MS.gene52644 | 0.810079 | 1.38E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene52644 | 0.829257 | 5.70E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene52644 | 0.834337 | 3.18E-56 | -1.69E-46 |
| MS.gene055555 | MS.gene52644 | 0.805071 | 1.59E-49 | -1.69E-46 |
| MS.gene055580 | MS.gene52644 | -0.842716 | 2.18E-58 | -1.69E-46 |
| MS.gene056043 | MS.gene52644 | -0.8445 | 7.27E-59 | -1.69E-46 |
| MS.gene057705 | MS.gene52644 | 0.806706 | 7.22E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene52644 | 0.801432 | 9.03E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene52644 | 0.801205 | 1.01E-48 | -1.69E-46 |
| MS.gene06001 | MS.gene52644 | 0.817331 | 3.49E-52 | -1.69E-46 |
| MS.gene060587 | MS.gene52644 | 0.822257 | 2.61E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene78355 | MS.gene52644 | PPI |
| MS.gene032141 | MS.gene52644 | PPI |
| MS.gene52644 | MS.gene04609 | PPI |
| MS.gene52644 | MS.gene017971 | PPI |
| MS.gene52644 | MS.gene31060 | PPI |
| MS.gene04609 | MS.gene52644 | PPI |
| MS.gene52644 | MS.gene032141 | PPI |
| MS.gene017971 | MS.gene52644 | PPI |
| MS.gene52644 | MS.gene78355 | PPI |
| MS.gene52644 | MS.gene072389 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52644.t1 | MTR_7g092970 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 9.50e-94 | 266 |
| MS.gene52644.t1 | MTR_2g098560 | 58.394 | 137 | 53 | 4 | 1 | 136 | 1 | 134 | 6.57e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52644.t1 | AT3G57930 | 51.748 | 143 | 56 | 5 | 1 | 135 | 1 | 138 | 1.09e-26 | 97.4 |
| MS.gene52644.t1 | AT3G57930 | 51.748 | 143 | 56 | 5 | 1 | 135 | 1 | 138 | 1.09e-26 | 97.4 |
| MS.gene52644.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
| MS.gene52644.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
| MS.gene52644.t1 | AT2G42190 | 45.652 | 138 | 72 | 2 | 1 | 135 | 1 | 138 | 3.61e-22 | 85.9 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTAATCACTGTGAATAAA+AGG | 0.302586 | 7.2:+16308293 | MS.gene52644:CDS |
| TCATCCGTGACCGGCTTTAA+TGG | 0.312272 | 7.2:-16308184 | None:intergenic |
| AAAGATGAGGAGAACGTAAA+TGG | 0.358180 | 7.2:+16308241 | MS.gene52644:CDS |
| GAACCAATGAAAGAGGAAAA+TGG | 0.383625 | 7.2:+16308352 | MS.gene52644:CDS |
| TCTACGGGATTCCGACAAAA+TGG | 0.386285 | 7.2:+16308412 | MS.gene52644:CDS |
| ATGGGTAGAGGTCGAGGAAA+GGG | 0.429359 | 7.2:+16308082 | MS.gene52644:CDS |
| GTAAATGGTAATGTTTCTAA+TGG | 0.448458 | 7.2:+16308256 | MS.gene52644:CDS |
| CCTCACCGTGCTGCTGAAGC+TGG | 0.469138 | 7.2:+16308454 | MS.gene52644:CDS |
| AAAGATAGAACCAATGAAAG+AGG | 0.492528 | 7.2:+16308345 | MS.gene52644:CDS |
| TCACTGTGAATAAAAGGAAA+AGG | 0.498884 | 7.2:+16308299 | MS.gene52644:CDS |
| TTGACTATGGGTAGAGGTCG+AGG | 0.519155 | 7.2:+16308076 | None:intergenic |
| TGAATAAAAGGAAAAGGAAG+AGG | 0.531731 | 7.2:+16308305 | MS.gene52644:CDS |
| TGGTGATTCAGTGAAGTCTA+CGG | 0.537967 | 7.2:+16308396 | MS.gene52644:CDS |
| CACCAGGGCTAGACTTAGCT+CGG | 0.543586 | 7.2:-16308378 | None:intergenic |
| GATTCCGACAAAATGGCAGC+AGG | 0.563142 | 7.2:+16308419 | MS.gene52644:CDS |
| GGTGATTCAGTGAAGTCTAC+GGG | 0.564571 | 7.2:+16308397 | MS.gene52644:CDS |
| TGGGTAGAGGTCGAGGAAAG+GGG | 0.567019 | 7.2:+16308083 | MS.gene52644:CDS |
| GAAGCAATCTGCAATTGTTG+AGG | 0.568281 | 7.2:+16308108 | MS.gene52644:CDS |
| GAAAAGGAAGAGGTCTTCAC+AGG | 0.568389 | 7.2:+16308315 | MS.gene52644:CDS |
| TATGGGTAGAGGTCGAGGAA+AGG | 0.570405 | 7.2:+16308081 | None:intergenic |
| GTCCGAGCTAAGTCTAGCCC+TGG | 0.574363 | 7.2:+16308376 | MS.gene52644:CDS |
| GGTTCTTTGACTATGGGTAG+AGG | 0.582744 | 7.2:+16308070 | None:intergenic |
| TAATGGTGATCTGAAAACTC+AGG | 0.592217 | 7.2:+16308273 | MS.gene52644:CDS |
| GAGACCATTAAAGCCGGTCA+CGG | 0.602292 | 7.2:+16308180 | MS.gene52644:CDS |
| AAGAGGGAGACCATTAAAGC+CGG | 0.607745 | 7.2:+16308174 | MS.gene52644:CDS |
| GTAGACTTCACTGAATCACC+AGG | 0.624371 | 7.2:-16308394 | None:intergenic |
| TGAAAAGATCGAGAAAGATG+AGG | 0.635309 | 7.2:+16308228 | MS.gene52644:CDS |
| CTACACCAGCTTCAGCAGCA+CGG | 0.678409 | 7.2:-16308459 | None:intergenic |
| CCAGCTTCAGCAGCACGGTG+AGG | 0.706272 | 7.2:-16308454 | None:intergenic |
| TAGACTTCACTGAATCACCA+GGG | 0.745429 | 7.2:-16308393 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAAATGGTAATGTTTCTAA+TGG | + | chr7.2:16308256-16308275 | MS.gene52644:CDS | 25.0% |
| AAAGATAGAACCAATGAAAG+AGG | + | chr7.2:16308345-16308364 | MS.gene52644:CDS | 30.0% | |
| AGGTAATCACTGTGAATAAA+AGG | + | chr7.2:16308293-16308312 | MS.gene52644:CDS | 30.0% | |
| TCACTGTGAATAAAAGGAAA+AGG | + | chr7.2:16308299-16308318 | MS.gene52644:CDS | 30.0% | |
| TGAATAAAAGGAAAAGGAAG+AGG | + | chr7.2:16308305-16308324 | MS.gene52644:CDS | 30.0% | |
| AAAGATGAGGAGAACGTAAA+TGG | + | chr7.2:16308241-16308260 | MS.gene52644:CDS | 35.0% | |
| GAACCAATGAAAGAGGAAAA+TGG | + | chr7.2:16308352-16308371 | MS.gene52644:CDS | 35.0% | |
| TAATGGTGATCTGAAAACTC+AGG | + | chr7.2:16308273-16308292 | MS.gene52644:CDS | 35.0% | |
| TGAAAAGATCGAGAAAGATG+AGG | + | chr7.2:16308228-16308247 | MS.gene52644:CDS | 35.0% | |
| ! | ACACCATTTTCCTCTTTCAT+TGG | - | chr7.2:16308358-16308377 | None:intergenic | 35.0% |
| GAAGCAATCTGCAATTGTTG+AGG | + | chr7.2:16308108-16308127 | MS.gene52644:CDS | 40.0% | |
| TACCAGCTCACAGAAAAAGA+GGG | + | chr7.2:16308158-16308177 | MS.gene52644:CDS | 40.0% | |
| TAGACTTCACTGAATCACCA+GGG | - | chr7.2:16308396-16308415 | None:intergenic | 40.0% | |
| TGGTGATTCAGTGAAGTCTA+CGG | + | chr7.2:16308396-16308415 | MS.gene52644:CDS | 40.0% | |
| AAGAGGGAGACCATTAAAGC+CGG | + | chr7.2:16308174-16308193 | MS.gene52644:CDS | 45.0% | |
| GAAAAGGAAGAGGTCTTCAC+AGG | + | chr7.2:16308315-16308334 | MS.gene52644:CDS | 45.0% | |
| GGTGATTCAGTGAAGTCTAC+GGG | + | chr7.2:16308397-16308416 | MS.gene52644:CDS | 45.0% | |
| GTACCAGCTCACAGAAAAAG+AGG | + | chr7.2:16308157-16308176 | MS.gene52644:CDS | 45.0% | |
| GTAGACTTCACTGAATCACC+AGG | - | chr7.2:16308397-16308416 | None:intergenic | 45.0% | |
| TCTACGGGATTCCGACAAAA+TGG | + | chr7.2:16308412-16308431 | MS.gene52644:CDS | 45.0% | |
| ! | TCTTCGTTTTCATCCGTGAC+CGG | - | chr7.2:16308196-16308215 | None:intergenic | 45.0% |
| ATGGGTAGAGGTCGAGGAAA+GGG | + | chr7.2:16308082-16308101 | MS.gene52644:CDS | 50.0% | |
| GAGACCATTAAAGCCGGTCA+CGG | + | chr7.2:16308180-16308199 | MS.gene52644:CDS | 50.0% | |
| GATTCCGACAAAATGGCAGC+AGG | + | chr7.2:16308419-16308438 | MS.gene52644:CDS | 50.0% | |
| ! | CTCCCTCTTTTTCTGTGAGC+TGG | - | chr7.2:16308163-16308182 | None:intergenic | 50.0% |
| ! | TCATCCGTGACCGGCTTTAA+TGG | - | chr7.2:16308187-16308206 | None:intergenic | 50.0% |
| !!! | TACGCCTGCTGCCATTTTGT+CGG | - | chr7.2:16308426-16308445 | None:intergenic | 50.0% |
| CACCAGGGCTAGACTTAGCT+CGG | - | chr7.2:16308381-16308400 | None:intergenic | 55.0% | |
| CTACACCAGCTTCAGCAGCA+CGG | - | chr7.2:16308462-16308481 | None:intergenic | 55.0% | |
| TGGGTAGAGGTCGAGGAAAG+GGG | + | chr7.2:16308083-16308102 | MS.gene52644:CDS | 55.0% | |
| GTCCGAGCTAAGTCTAGCCC+TGG | + | chr7.2:16308376-16308395 | MS.gene52644:CDS | 60.0% | |
| CCAGCTTCAGCAGCACGGTG+AGG | - | chr7.2:16308457-16308476 | None:intergenic | 65.0% | |
| ! | CCTCACCGTGCTGCTGAAGC+TGG | + | chr7.2:16308454-16308473 | MS.gene52644:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 16308082 | 16308492 | 16308082 | ID=MS.gene52644 |
| chr7.2 | mRNA | 16308082 | 16308492 | 16308082 | ID=MS.gene52644.t1;Parent=MS.gene52644 |
| chr7.2 | exon | 16308082 | 16308492 | 16308082 | ID=MS.gene52644.t1.exon1;Parent=MS.gene52644.t1 |
| chr7.2 | CDS | 16308082 | 16308492 | 16308082 | ID=cds.MS.gene52644.t1;Parent=MS.gene52644.t1 |
| Gene Sequence |
| Protein sequence |
