AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049639

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049639.t1 MTR_3g063520 91.679 685 47 1 1 675 1 685 0.0 1204
MS.gene049639.t1 MTR_5g091600 87.482 687 64 3 1 675 1 677 0.0 1190
MS.gene049639.t1 MTR_3g063490 85.239 691 86 2 1 675 1 691 0.0 1177
MS.gene049639.t1 MTR_6g077870 70.030 664 183 5 24 675 48 707 0.0 956
MS.gene049639.t1 MTR_6g077870 69.091 550 154 5 138 675 1 546 0.0 783
MS.gene049639.t1 MTR_7g028250 56.878 647 263 6 39 671 35 679 0.0 749
MS.gene049639.t1 MTR_3g092090 53.028 677 286 8 2 665 6 663 0.0 719
MS.gene049639.t1 MTR_3g092090 52.853 666 282 8 2 654 6 652 0.0 703
MS.gene049639.t1 MTR_0312s0020 50.681 661 305 6 21 668 20 672 0.0 687
MS.gene049639.t1 MTR_1g007540 50.681 661 305 6 21 668 20 672 0.0 687
MS.gene049639.t1 MTR_1g007580 50.445 674 305 8 19 668 4 672 0.0 681
MS.gene049639.t1 MTR_1g077840 50.470 638 299 5 42 667 47 679 0.0 679
MS.gene049639.t1 MTR_5g091615 90.446 157 15 0 517 673 59 215 7.12e-88 281
MS.gene049639.t1 MTR_1626s0010 49.787 235 115 1 210 444 4 235 7.25e-77 249
MS.gene049639.t1 MTR_4g074280 84.466 103 16 0 701 803 74 176 2.04e-53 187
MS.gene049639.t1 MTR_4g074300 71.429 119 33 1 701 818 75 193 1.26e-49 177
MS.gene049639.t1 MTR_4g074330 62.136 103 38 1 701 803 98 199 2.32e-32 128
MS.gene049639.t1 MTR_0312s0050 48.780 123 52 3 16 127 1 123 6.43e-31 118
MS.gene049639.t1 MTR_3g063510 87.500 48 6 0 701 748 14 61 4.92e-23 94.0
MS.gene049639.t1 MTR_2g083230 89.130 46 5 0 129 174 34 79 2.11e-18 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049639.t1 AT1G65730 74.850 668 155 3 20 675 16 682 0.0 1033
MS.gene049639.t1 AT3G17650 70.783 664 175 4 29 675 46 707 0.0 930
MS.gene049639.t1 AT3G17650 70.783 664 175 4 29 675 46 707 0.0 930
MS.gene049639.t1 AT1G48370 69.774 665 181 3 29 675 55 717 0.0 914
MS.gene049639.t1 AT3G27020 58.089 649 255 6 38 671 30 676 0.0 757
MS.gene049639.t1 AT1G48370 67.071 577 156 4 138 696 1 561 0.0 749
MS.gene049639.t1 AT5G41000 54.923 650 268 7 35 665 24 667 0.0 721
MS.gene049639.t1 AT5G53550 53.582 670 280 7 21 666 7 669 0.0 712
MS.gene049639.t1 AT5G53550 53.582 670 280 7 21 666 7 669 0.0 712
MS.gene049639.t1 AT5G53550 53.582 670 280 7 21 666 7 669 0.0 712
MS.gene049639.t1 AT5G53550 53.582 670 280 7 21 666 7 669 0.0 712
MS.gene049639.t1 AT5G53550 53.582 670 280 7 21 666 7 669 0.0 712
MS.gene049639.t1 AT4G24120 52.135 679 298 7 1 667 9 672 0.0 705
MS.gene049639.t1 AT5G24380 52.280 658 296 6 22 665 7 660 0.0 699
MS.gene049639.t1 AT5G41000 52.713 645 265 6 35 665 24 642 0.0 681
MS.gene049639.t1 AT5G53550 56.226 514 208 4 163 666 1 507 0.0 583
MS.gene049639.t1 AT5G41000 54.326 497 205 6 185 665 1 491 0.0 553
MS.gene049639.t1 AT5G45450 67.442 215 64 1 461 675 1 209 3.25e-94 294
MS.gene049639.t1 AT3G18630 57.143 105 43 1 701 803 123 227 2.08e-31 126

Find 187 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTTGTTGTTAGCTTTCT+TGG 0.055871 3.2:-59409642 MS.gene049639:CDS
AATGTTTCTGCTGGGTTGTT+AGG 0.216597 3.2:-59410082 MS.gene049639:CDS
CTTAAGTACATTCTTCAAAA+TGG 0.223742 3.2:+59404131 None:intergenic
TACAACTTTGTGAAAGTTCT+TGG 0.229178 3.2:-59406871 MS.gene049639:CDS
GAAGCTTGATATTGTTTATT+TGG 0.238786 3.2:-59406018 MS.gene049639:CDS
GAGGAGATTTCTGTGGAAAA+AGG 0.246179 3.2:-59410241 MS.gene049639:CDS
AAAGTTGACGTATTTCTTTC+CGG 0.246477 3.2:+59406111 None:intergenic
ATGTTTGTCAGCCAAGTTAT+TGG 0.252422 3.2:-59406361 MS.gene049639:CDS
TGATTTGTGGAGATGGAATT+TGG 0.269346 3.2:-59405940 MS.gene049639:CDS
GTATTCTCTTGTCTGGTAAA+AGG 0.281889 3.2:+59410001 None:intergenic
GCTTCACCAGGTCCAGAATA+AGG 0.283982 3.2:+59404198 None:intergenic
AGCGAAATATTGATTATATA+TGG 0.302737 3.2:+59407330 None:intergenic
AAATTAGCCATCTTTGCTAT+TGG 0.329826 3.2:-59406523 MS.gene049639:CDS
CCATCTTTGCTATTGGTGCT+TGG 0.330506 3.2:-59406516 MS.gene049639:CDS
CATCTTTGCTATTGGTGCTT+GGG 0.333763 3.2:-59406515 MS.gene049639:CDS
ATCAATATTTCGCTGTTGAT+TGG 0.338595 3.2:-59407321 MS.gene049639:CDS
CTCAAACAAAGCAGCTTTCA+TGG 0.339727 3.2:-59407228 MS.gene049639:CDS
TCATTGGCAGTGTCAAATTC+TGG 0.345905 3.2:+59409702 None:intergenic
CCTTCGTTGAATGTTTCTGC+TGG 0.354500 3.2:-59410091 MS.gene049639:CDS
AAGTAGCTTCCAATGTAAAA+TGG 0.358086 3.2:+59406061 None:intergenic
TGGCTGACAAACATTGATTT+CGG 0.360151 3.2:+59406370 None:intergenic
TTTGCAAACTTCCCCACATT+TGG 0.363476 3.2:-59407671 MS.gene049639:CDS
CTTCTCCTTTAGCTTCTTGT+GGG 0.366991 3.2:-59407735 MS.gene049639:CDS
AGGGCTAAGGCTGATGCATT+TGG 0.367842 3.2:-59405983 MS.gene049639:CDS
AAGGAGGTTAAGGATGATAT+TGG 0.368153 3.2:-59404111 MS.gene049639:CDS
CTAACGGATTGGTCCCTTGC+AGG 0.370494 3.2:-59406556 MS.gene049639:CDS
TTATTTGGCAGAAGGTTGAT+AGG 0.371137 3.2:-59406003 MS.gene049639:CDS
TGGATATTCTACAATGCTTT+CGG 0.371623 3.2:-59406301 MS.gene049639:CDS
GACAAGAATTTCATGCAAAT+AGG 0.377015 3.2:+59405875 None:intergenic
CTGCTTTGTTTGAGATCAGC+AGG 0.384597 3.2:+59407237 None:intergenic
TGTTCCAATGTGGCAGAAAC+AGG 0.384686 3.2:-59410203 MS.gene049639:CDS
CAACTTTATTCCTGTTCCTA+TGG 0.386872 3.2:-59406095 MS.gene049639:CDS
TGATTGGTGGAATTCTTTCA+TGG 0.388694 3.2:-59407305 MS.gene049639:CDS
GAATGTACTTAAGGAGGTTA+AGG 0.394852 3.2:-59404121 MS.gene049639:CDS
GCCAATGATGTTAAGAGTCT+TGG 0.398551 3.2:-59409687 MS.gene049639:CDS
ATGAAGTTGAATCTCACAAC+TGG 0.401497 3.2:-59410121 MS.gene049639:CDS
TTATTAATGCTGTTCGCGAC+TGG 0.406081 3.2:-59406135 MS.gene049639:CDS
GTTTGGCACCTTCAGATGTA+TGG 0.407013 3.2:+59407792 None:intergenic
TATTCCTGTTCCTATGGCTA+TGG 0.408926 3.2:-59406089 MS.gene049639:CDS
GTAGGTGGGAGAGGAGCATT+TGG 0.412469 3.2:+59406784 None:intergenic
ATTCGCATACTTGTGTTGAT+AGG 0.414944 3.2:+59407640 None:intergenic
ACTTTCAAATACATATTTCC+AGG 0.415857 3.2:-59403033 MS.gene049639:CDS
TCTGTTAAGGCTGTGATCCT+TGG 0.417053 3.2:-59404305 MS.gene049639:CDS
GATGTTAAGAGTCTTGGTTT+AGG 0.418370 3.2:-59409681 MS.gene049639:CDS
TATCGAAACATTGCAATACT+AGG 0.418521 3.2:-59406229 MS.gene049639:CDS
TAAACAAGTGCGTACGGTGC+AGG 0.418987 3.2:+59406250 None:intergenic
TGGTCCCTTGCAGGGACTTA+CGG 0.421184 3.2:-59406547 MS.gene049639:CDS
AGAGGAGCATTTGGATCATC+AGG 0.423631 3.2:+59406793 None:intergenic
TACAATGCTTTCGGTACTCT+TGG 0.425617 3.2:-59406292 MS.gene049639:CDS
GAAGACACATTTGCTATGGT+TGG 0.428517 3.2:-59403063 MS.gene049639:CDS
AATAACAGTATTCTCTTGTC+TGG 0.429956 3.2:+59409994 None:intergenic
GATTGGTGGAATTCTTTCAT+GGG 0.431679 3.2:-59407304 MS.gene049639:CDS
TAAGGCTGTGATCCTTGGCC+AGG 0.432505 3.2:-59404300 MS.gene049639:intron
TCTGGACCTGGTGAAGCAAT+GGG 0.436972 3.2:-59404192 MS.gene049639:CDS
TCTTGTGGGGTCTCTTCCAA+TGG 0.439451 3.2:-59407721 MS.gene049639:CDS
TATTAATGCTGTTCGCGACT+GGG 0.441132 3.2:-59406134 MS.gene049639:CDS
TCTTCTCCTTTAGCTTCTTG+TGG 0.441527 3.2:-59407736 MS.gene049639:CDS
ACCAAGACTCTTAACATCAT+TGG 0.446254 3.2:+59409686 None:intergenic
ATGTTTCTGCTGGGTTGTTA+GGG 0.449011 3.2:-59410081 MS.gene049639:CDS
TTCACTGCTGGTGATGCTTG+TGG 0.449951 3.2:-59407695 MS.gene049639:CDS
CTCATGCAAGACTTCAAAAC+AGG 0.450675 3.2:-59406412 MS.gene049639:CDS
ACTAGGTGTGAACGGATTCT+CGG 0.450705 3.2:-59406212 MS.gene049639:CDS
TTTGGATCAGCTGTTGCGTC+CGG 0.451755 3.2:-59405965 MS.gene049639:CDS
GGATGGAAATCTGACTCCCT+TGG 0.453443 3.2:+59404152 None:intergenic
GGTGTCATGTGGCCTCTCAT+TGG 0.456041 3.2:-59407282 MS.gene049639:CDS
TTAAGAGTCTTGGTTTAGGA+TGG 0.456468 3.2:-59409677 MS.gene049639:CDS
CCATGGCAGTTCCAATAACT+TGG 0.463576 3.2:+59406350 None:intergenic
GTTGCGTCCGGTTTGATTTG+TGG 0.469197 3.2:-59405953 MS.gene049639:CDS
CCAAGCACCAATAGCAAAGA+TGG 0.469862 3.2:+59406516 None:intergenic
AATGCTGTTCGCGACTGGGC+CGG 0.470037 3.2:-59406130 MS.gene049639:CDS
AGCAATGGGGCTATCATTCT+CGG 0.470396 3.2:-59404178 MS.gene049639:CDS
TTCTGGACCTGGTGAAGCAA+TGG 0.470532 3.2:-59404193 MS.gene049639:CDS
GGCAGAAACAGGTTACCGTT+AGG 0.470544 3.2:-59410192 MS.gene049639:CDS
CAAGTTATTGGAACTGCCAT+GGG 0.471749 3.2:-59406349 MS.gene049639:CDS
CTTCGTTGAATGTTTCTGCT+GGG 0.472472 3.2:-59410090 MS.gene049639:CDS
AGTACATTCTTCAAAATGGA+TGG 0.477112 3.2:+59404135 None:intergenic
GATGGAAATCTGACTCCCTT+GGG 0.488704 3.2:+59404153 None:intergenic
AAACTTCCCCACATTTGGTC+TGG 0.492239 3.2:-59407666 MS.gene049639:CDS
ATGCAAAGAGCAGGGGCGTA+AGG 0.493873 3.2:+59404419 None:intergenic
TGACGTGATACCACTTTAGC+TGG 0.494074 3.2:+59406635 None:intergenic
AATATTTCGCTGTTGATTGG+TGG 0.495302 3.2:-59407318 MS.gene049639:CDS
TCCGGTTTGATTTGTGGAGA+TGG 0.495372 3.2:-59405947 MS.gene049639:CDS
ATGATGGGATTTGATCTTTG+AGG 0.497225 3.2:+59406725 None:intergenic
TTGAAAATCAAATGCGGAGG+AGG 0.498546 3.2:+59404353 None:intergenic
TTCTTTCATGGGGTGTCATG+TGG 0.500744 3.2:-59407293 MS.gene049639:CDS
TCCATCTCCACAAATCAAAC+CGG 0.501528 3.2:+59405946 None:intergenic
CTGCGATTGCGAACCATGAT+GGG 0.503073 3.2:+59406710 None:intergenic
ATAGCCATAGCCATAGGAAC+AGG 0.504745 3.2:+59406085 None:intergenic
ACTTGTGTTGTTGCCGCTAG+TGG 0.505150 3.2:-59409968 MS.gene049639:CDS
ACCAGCTTTCTCAAGCAGCT+TGG 0.505163 3.2:+59410036 None:intergenic
GATATTGTTTATTTGGCAGA+AGG 0.507191 3.2:-59406011 MS.gene049639:CDS
TTTGCTATTGATATGTGTGT+TGG 0.507636 3.2:-59406040 MS.gene049639:CDS
GCAGAAACAGGTTACCGTTA+GGG 0.508322 3.2:-59410191 MS.gene049639:CDS
CTTTGAGGAACATCTCGGTT+CGG 0.512197 3.2:+59406740 None:intergenic
TTCGGTACTCTTGGTCAACC+TGG 0.515517 3.2:-59406283 MS.gene049639:CDS
ACTTTAGCTGGTGGAAAATG+TGG 0.515525 3.2:+59406647 None:intergenic
CAAAACAGGTTACATGACAT+TGG 0.515553 3.2:-59406398 MS.gene049639:CDS
ATTAGAAGACACATTTGCTA+TGG 0.516131 3.2:-59403067 MS.gene049639:CDS
CCATCATGGTTCGCAATCGC+AGG 0.516456 3.2:-59406709 MS.gene049639:CDS
AAACAAGTGCGTACGGTGCA+GGG 0.517076 3.2:+59406251 None:intergenic
CCGACGAACATGCAAAGAGC+AGG 0.517254 3.2:+59404410 None:intergenic
TGAGCTGTTGCGGTACCACT+TGG 0.521215 3.2:+59407830 None:intergenic
ACCAAGCTGCTTGAGAAAGC+TGG 0.521284 3.2:-59410037 MS.gene049639:CDS
TATTTGGCAGAAGGTTGATA+GGG 0.522764 3.2:-59406002 MS.gene049639:CDS
GCGAACAGCATTAATAATCA+TGG 0.528967 3.2:+59406141 None:intergenic
GACACCACATGCTGCTAGAC+CGG 0.529825 3.2:+59406465 None:intergenic
GTAACCTGTTTCTGCCACAT+TGG 0.532506 3.2:+59410199 None:intergenic
CCAAGTTATTGGAACTGCCA+TGG 0.533495 3.2:-59406350 MS.gene049639:CDS
TTCAGTGGTTCCACTCCGAA+AGG 0.534003 3.2:-59409613 MS.gene049639:intron
GCTATCATTCTCGGCTCCCA+AGG 0.536562 3.2:-59404169 MS.gene049639:CDS
TGCCTATGGTTGTGGACTAA+CGG 0.537350 3.2:-59406572 MS.gene049639:CDS
ATAGCCATAGCAATGATCCT+TGG 0.540258 3.2:-59406904 MS.gene049639:CDS
TAGGAACCTTATTCTGGACC+TGG 0.541732 3.2:-59404204 MS.gene049639:CDS
TTTGAAGAATGTACTTAAGG+AGG 0.542193 3.2:-59404127 MS.gene049639:CDS
AATGAAATTGTAGGTGGGAG+AGG 0.543345 3.2:+59406775 None:intergenic
TTGTTGTCGAATGAAATTGT+AGG 0.544030 3.2:+59406766 None:intergenic
GCATTGCAAAAGGCTAGTGC+AGG 0.548633 3.2:+59406592 None:intergenic
CCTGCGATTGCGAACCATGA+TGG 0.550308 3.2:+59406709 None:intergenic
CCTGCTCTTTGCATGTTCGT+CGG 0.551374 3.2:-59404410 MS.gene049639:CDS
GCAGAAGGTTGATAGGGCTA+AGG 0.554139 3.2:-59405996 MS.gene049639:CDS
CTTTAGCTGGTGGAAAATGT+GGG 0.554313 3.2:+59406648 None:intergenic
TTGATCTTTGAGGAACATCT+CGG 0.554704 3.2:+59406735 None:intergenic
ATGGTTGTGGACTAACGGAT+TGG 0.556934 3.2:-59406567 MS.gene049639:CDS
ACAGCCTTAACAGAATGAAA+AGG 0.557732 3.2:+59404314 None:intergenic
ATTTGTGAGTCTCATGTTGT+CGG 0.557992 3.2:-59410164 MS.gene049639:CDS
AACAGTTTCCATACATCTGA+AGG 0.562004 3.2:-59407800 MS.gene049639:intron
CGACACCGGCGCAACTGCGA+AGG 0.562854 3.2:+59410279 None:intergenic
TCGTGACTTTCGATGTCGTA+GGG 0.563493 3.2:+59410304 None:intergenic
ACAACCATAGGCATTGCAAA+AGG 0.564312 3.2:+59406582 None:intergenic
GAGACCCCACAAGAAGCTAA+AGG 0.566191 3.2:+59407730 None:intergenic
CGACACCTTCGCAGTTGCGC+CGG 0.567012 3.2:-59410284 MS.gene049639:CDS
TAACGGATTGGTCCCTTGCA+GGG 0.567512 3.2:-59406555 MS.gene049639:CDS
ATAGGCTTCCAGACCAAATG+TGG 0.571043 3.2:+59407658 None:intergenic
GCAAGAGCAAGAAAAGAGCT+AGG 0.577753 3.2:+59405911 None:intergenic
TCAAAGATCAAATCCCATCA+TGG 0.579940 3.2:-59406723 MS.gene049639:CDS
CGTTGTTCATCTTTCGACAC+CGG 0.584469 3.2:+59410265 None:intergenic
ATCCGTTAGTCCACAACCAT+AGG 0.586179 3.2:+59406570 None:intergenic
ATCACCAAGGATCATTGCTA+TGG 0.588439 3.2:+59406900 None:intergenic
GCTAACGGTGGTGTCCTAGC+CGG 0.589089 3.2:-59406484 MS.gene049639:CDS
TTCTCCTTTAGCTTCTTGTG+GGG 0.590056 3.2:-59407734 MS.gene049639:CDS
AAGAATTTCATGCAAATAGG+TGG 0.591699 3.2:+59405878 None:intergenic
GTCAAGAGCAGCAAATGCTA+AGG 0.594984 3.2:-59405855 MS.gene049639:intron
AATGGTATAGCCATAGCCAT+AGG 0.595250 3.2:+59406079 None:intergenic
AGCTGCTTGAGAAAGCTGGT+TGG 0.595820 3.2:-59410033 MS.gene049639:CDS
GAAATCCACAAACCTGGCCA+AGG 0.595919 3.2:+59404288 None:intergenic
ACTCACAAATATTGCCCTAA+CGG 0.597485 3.2:+59410177 None:intergenic
CTATCATTCTCGGCTCCCAA+GGG 0.600556 3.2:-59404168 MS.gene049639:CDS
CGACGAACATGCAAAGAGCA+GGG 0.604175 3.2:+59404411 None:intergenic
GGTGCAGGGTATGCTGTACC+AGG 0.605516 3.2:+59406265 None:intergenic
GATAGCCCCATTGCTTCACC+AGG 0.605919 3.2:+59404186 None:intergenic
ATTGGTGGAATTCTTTCATG+GGG 0.611593 3.2:-59407303 MS.gene049639:CDS
ATTGCAATACTAGGTGTGAA+CGG 0.619741 3.2:-59406220 MS.gene049639:CDS
GTCGTGACTTTCGATGTCGT+AGG 0.620908 3.2:+59410303 None:intergenic
TTTCGATAAACAAGTGCGTA+CGG 0.622133 3.2:+59406244 None:intergenic
ATAGCAATGATCCTTGGTGA+TGG 0.624209 3.2:-59406898 MS.gene049639:CDS
CGTGATACCACTTTAGCTGG+TGG 0.624435 3.2:+59406638 None:intergenic
TTGTCGAATGAAATTGTAGG+TGG 0.626285 3.2:+59406769 None:intergenic
TGCTTTGTTTGAGATCAGCA+GGG 0.627385 3.2:+59407238 None:intergenic
GACGAACATGCAAAGAGCAG+GGG 0.641386 3.2:+59404412 None:intergenic
GTTGATTAGATGAGCTGTTG+CGG 0.643794 3.2:+59407820 None:intergenic
TTTAGCTGGTGGAAAATGTG+GGG 0.643964 3.2:+59406649 None:intergenic
GCTTGGGCACGTGAAGCTAA+CGG 0.644029 3.2:-59406499 MS.gene049639:CDS
AGTTAGTGTACCTTTCGGAG+TGG 0.644340 3.2:+59409603 None:intergenic
CGGCGATATAACACACCCCA+TGG 0.644717 3.2:+59406333 None:intergenic
CACATGCTGCTAGACCGGCT+AGG 0.645357 3.2:+59406470 None:intergenic
TTCAAATTGACATATCCAAG+TGG 0.649978 3.2:-59407845 MS.gene049639:CDS
TAGGCTTCCAGACCAAATGT+GGG 0.650528 3.2:+59407659 None:intergenic
GTCGAAAGATGAACAACGTG+AGG 0.655792 3.2:-59410260 MS.gene049639:CDS
TGTCGAATGAAATTGTAGGT+GGG 0.661752 3.2:+59406770 None:intergenic
CTAGCCGGTCTAGCAGCATG+TGG 0.668907 3.2:-59406469 MS.gene049639:CDS
TGAAGTTGAATCTCACAACT+GGG 0.670966 3.2:-59410120 MS.gene049639:CDS
TTAGCTGGTGGAAAATGTGG+GGG 0.673842 3.2:+59406650 None:intergenic
TAGAATATCCAGAAAACACA+CGG 0.679060 3.2:+59406313 None:intergenic
GCATTGAAAATCAAATGCGG+AGG 0.680649 3.2:+59404350 None:intergenic
ACAACGTGAGGAGATTTCTG+TGG 0.682535 3.2:-59410248 MS.gene049639:CDS
GCTGAATGCAATTCCACTAG+CGG 0.682644 3.2:+59409955 None:intergenic
AAGGAACATATGTTCCAATG+TGG 0.686321 3.2:-59410213 MS.gene049639:CDS
AAAGCATTGAAAATCAAATG+CGG 0.686416 3.2:+59404347 None:intergenic
CTGGACCTGGTGAAGCAATG+GGG 0.690829 3.2:-59404191 MS.gene049639:CDS
CCAGCAGAAACATTCAACGA+AGG 0.700492 3.2:+59410091 None:intergenic
GCTAGTGGAATTGCATTCAG+CGG 0.703902 3.2:-59409953 MS.gene049639:intron
AGGCTTCCAGACCAAATGTG+GGG 0.704515 3.2:+59407660 None:intergenic
AAAACACGATGCAAAGTACG+AGG 0.716918 3.2:+59406173 None:intergenic
AAGTTATTGGAACTGCCATG+GGG 0.727985 3.2:-59406348 MS.gene049639:CDS
TGGGCACGTGAAGCTAACGG+TGG 0.748558 3.2:-59406496 MS.gene049639:CDS
TGGGTCTAACAAGTACAAAG+CGG 0.750344 3.2:-59404603 MS.gene049639:intron
AGTTGTATAGACCATCACCA+AGG 0.762040 3.2:+59406887 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.2 gene 59403017 59410338 59403017 ID=MS.gene049639
chr3.2 mRNA 59403017 59410338 59403017 ID=MS.gene049639.t1;Parent=MS.gene049639
chr3.2 exon 59409954 59410338 59409954 ID=MS.gene049639.t1.exon1;Parent=MS.gene049639.t1
chr3.2 CDS 59409954 59410338 59409954 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59409614 59409777 59409614 ID=MS.gene049639.t1.exon2;Parent=MS.gene049639.t1
chr3.2 CDS 59409614 59409777 59409614 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59407801 59407882 59407801 ID=MS.gene049639.t1.exon3;Parent=MS.gene049639.t1
chr3.2 CDS 59407801 59407882 59407801 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59407650 59407770 59407650 ID=MS.gene049639.t1.exon4;Parent=MS.gene049639.t1
chr3.2 CDS 59407650 59407770 59407650 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59407212 59407401 59407212 ID=MS.gene049639.t1.exon5;Parent=MS.gene049639.t1
chr3.2 CDS 59407212 59407401 59407212 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59405856 59406932 59405856 ID=MS.gene049639.t1.exon6;Parent=MS.gene049639.t1
chr3.2 CDS 59405856 59406932 59405856 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59404604 59404684 59404604 ID=MS.gene049639.t1.exon7;Parent=MS.gene049639.t1
chr3.2 CDS 59404604 59404684 59404604 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59404301 59404444 59404301 ID=MS.gene049639.t1.exon8;Parent=MS.gene049639.t1
chr3.2 CDS 59404301 59404444 59404301 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59404065 59404223 59404065 ID=MS.gene049639.t1.exon9;Parent=MS.gene049639.t1
chr3.2 CDS 59404065 59404223 59404065 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
chr3.2 exon 59403017 59403124 59403017 ID=MS.gene049639.t1.exon10;Parent=MS.gene049639.t1
chr3.2 CDS 59403017 59403124 59403017 ID=cds.MS.gene049639.t1;Parent=MS.gene049639.t1
Gene Sequence

>MS.gene049639

ATGTCTCGAGAACCCTACGACATCGAAAGTCACGACACCTTCGCAGTTGCGCCGGTGTCGAAAGATGAACAACGTGAGGAGATTTCTGTGGAAAAAGGGTTTGAAGGAACATATGTTCCAATGTGGCAGAAACAGGTTACCGTTAGGGCAATATTTGTGAGTCTCATGTTGTCGGTGATGTTCACGTTCATCGTGATGAAGTTGAATCTCACAACTGGGATTATGCCTTCGTTGAATGTTTCTGCTGGGTTGTTAGGGTTTTTCTTTGTAAAGACATGGACCAAGCTGCTTGAGAAAGCTGGTTGGCTTAATCAACCTTTTACCAGACAAGAGAATACTGTTATTCAGACTTGTGTTGTTGCCGCTAGTGGAATTGCATTCAGCGGTAGTTTTTCTTACCTAGTTGTAGATGTTACTTTACTTCAATTTAATACTTGACTCTGAGTTGAATAAATATTATATGCCCTAATAGTTTTGTGAACAAGTTTATGCTCTAATAGTTAAATGCAGTTCTGATACAGTGTCTTTGTTTGTCGTTGATAAATCTTATTGTTTGTGTAGGTGGATTTTGTAGCAACTTGTTTGGAATGAGTCCAACTATTGCAAAACAAATTCCAGAATTTGACACTGCCAATGATGTTAAGAGTCTTGGTTTAGGATGGATGATTGCATTTCTATTTGTTGTTAGCTTTCTTGGTCTCTTTTCAGTGGTTCCACTCCGAAAGGTACACTAACTTATTACACCTACCTTAATAAAATGTAACCTTCAACACTAGAATATAGGCCTTTTTAATTATTTGAATTTTGCATTGATATACTCCCTCCGTTCCTTTTTAAATGTTTTTTTTAGAAAATTAACTCCAAAATAGCAATGTGTATTTTTGCACAGTTTCTTCTATTATACCCTCATTTTAATCCACCAATAAATTCCTATTATTTTGCACACACTTTATCTTTCCAATAAATTTAGTTATATTTCTCTCGTAACAAACAATTGTCAGTTATTATCTTTTTCTCCAACAAATTCCTATTTTTTTTTGCGCGCTCTTTCTCTCGTAACAAACAAGTATCAATTTTCTAAATATTGTGCACATTTTTATAATTATTTTAAGTCAATATAAAAATGATTCCAACTTCTTCTTCTTCATTGTCATCTTGCTCTTGTTCTTGTATCTTATGAAATGAGTTTTCAACTATTTCTCCCTCTTCATCTCTCATCAGCAGTTCCAATGAATCTTCTACTTTTTCTTCCTTTTAAAAATTTATGGAATCTTTCATGGTTCTTTTACTTTGCAATTGAAATTTGTAAAATTTGTTACTCATATTATTTATAGATTTTTAACAGTTCTTGGAACCATTGTTTGTAACAAAATTTTGTTTAAATTTGAAGATAACAAGAAAAAAAACTGTTTTTTTCTTAAAAAACAAAAAAAAAACAAATTGTTTAAAAAAAACAAACTTTAGTTTTCCAAATAGATAACATTAATTAGTTTTATTGAAAAAATAAGAGTAATTGACAAAGGGTATAATTGACAAATTGTACCATTAAAATACCAAAAAGACGGTTAAAAAAAAACTGAGGAAGTATTTGCTTGCATTGCATGACATATATCTTTTCTATGAATGAACTATATTTAAAAAATTGACTAATGACTTTCTACAAGAAAACTGCCATATACCTACAGAGGTTTATAGTGACAGCTACTGAAACCGTAGGTATTGAAAATATATACCCACCGAAAATGCATTGACGTAGCTATAGACATAAAAATAAAAAAACAAAAGGTTCTGAATAATTTCCCCAAAAAAAAGGTTCTGAATAATTTTGCTTCAGATTTTGATTATTGCTTCATTTTAATAATTGTTGTGTTAATTTGAATCTATTAGTTGTGTTATGTTAATTTGAATTGCAGATTATATGAGTTGTGTTGTGTTAATTTTGTTTCTATGAGATTCTGGAGAACCCTAGGTTACCCAACTATTCTAGAACTTTGCATGTTAACTTGTCAATAATTACAAGTTAAATTGTTTGAATGTGACTTAATTAAATTAATATCACTGGGTTGATACAAGTTGGGTTATGTGTATTCTCTATTAAGCCCAAACACTTCCTTCCACTGGACATGACACACACCAACATAATCTAGGACACTGGGAAACTGCATGTGTGTCCTTGTATATATGAGTCCCATGTTATGTATTCTCTAGTGCTGTTGTTGCCTTGGACTAGTTTCATAAGTTTTTTTTTTTTTTTTTTTTTTGTAATAAGTAGGTGTAGGTATTTTGGTGCTATACCTACGGAAAATGACATGTACCTACGGTTTTACGCTGTCAATAAAAGTCAATTTTCTTGCAGTGTGGATACCTCGTTGCAGCGATATTTGAATCAAGTTTTAAAACACGGGATGGGAATGACTCCAAACATTTAGTTTTAATTTAATGTTTCCTTGTTGTAGATTATGATTGTTGACTTCAAATTGACATATCCAAGTGGTACCGCAACAGCTCATCTAATCAACAGTTTCCATACATCTGAAGGTGCCAAACTAGCAAAGAAGCAAGTACAAGCACTTGGAAAGTTCTTCTCCTTTAGCTTCTTGTGGGGTCTCTTCCAATGGTTTTTCACTGCTGGTGATGCTTGTGGATTTGCAAACTTCCCCACATTTGGTCTGGAAGCCTATCAACACAAGTATGCGAATCGCCTTTATTTTATTAATTAATGAGAGAATTCAAAAAGGGATTATTAACTCTGTTACTTGGATTATAAACTACACAATATAAAAGCTGATCGAAACTGAAACCTCTATGTTAATTATGGTGATAGCATGATGAGTTGCCAACTTATTTAGATAATATTGATGTGATAAAATAGATTAATAATATGTATTTTAGTTCCTTTTCTTTTAGCTAACCTTTATTATTTAATGTACATAACAGGTTCTACTTTGATTTTTCTGCAACCTATGTGGGGGTTGGGATGATATGTCCATATATAATCAATATTTCGCTGTTGATTGGTGGAATTCTTTCATGGGGTGTCATGTGGCCTCTCATTGGTGCTAAAAAAGGAGATTGGTTCCCTGCTGATCTCAAACAAAGCAGCTTTCATGGTCTTCAAGCTTACAAAGTAAGTTGTCTTTCTACATTTCAATTTTTTATTTATTTAAATTGTTGGCGAAAATCTACATTTTTGACAATACTCCACTTTTTGTAAGTATACTTTGGAATTGGTATAGAAAAATAGTAGTTTATCAATGGATATCAACTTAAAATACAGTTAATTGGATTGAAACAAGTACAAAACTGGAATTTGGACATACTCTGATGCAATAGATCTTTTTGAATGTGACTGGTTGATAATGTATTCTTTTGGCAGTTTTCTAATTGTATTATTTTTAAATCACAGGTTTTCATAGCCATAGCAATGATCCTTGGTGATGGTCTATACAACTTTGTGAAAGTTCTTGGCACAACTCTTTTAGGTTTGTATAAACAATGGAAGAAAAAAGATAAGAGAGCACAACCTGATGATCCAAATGCTCCTCTCCCACCTACAATTTCATTCGACAACAAGCGCCGAACCGAGATGTTCCTCAAAGATCAAATCCCATCATGGTTCGCAATCGCAGGCTATGTCATAATTGCAATAATCTCAACCATTACTATCCCCCACATTTTCCACCAGCTAAAGTGGTATCACGTCATTTGCATTTACATCATTGCACCTGCACTAGCCTTTTGCAATGCCTATGGTTGTGGACTAACGGATTGGTCCCTTGCAGGGACTTACGGAAAATTAGCCATCTTTGCTATTGGTGCTTGGGCACGTGAAGCTAACGGTGGTGTCCTAGCCGGTCTAGCAGCATGTGGTGTCATGATGAACATTGTTTCAACAGCTTCTGATCTCATGCAAGACTTCAAAACAGGTTACATGACATTGGCTTCTCCGAAATCAATGTTTGTCAGCCAAGTTATTGGAACTGCCATGGGGTGTGTTATATCGCCGTGTGTTTTCTGGATATTCTACAATGCTTTCGGTACTCTTGGTCAACCTGGTACAGCATACCCTGCACCGTACGCACTTGTTTATCGAAACATTGCAATACTAGGTGTGAACGGATTCTCGGCTTTTCCCAAAAACTGCCTCGTACTTTGCATCGTGTTTTTTATTGGTGCCATGATTATTAATGCTGTTCGCGACTGGGCCGGAAAGAAATACGTCAACTTTATTCCTGTTCCTATGGCTATGGCTATACCATTTTACATTGGAAGCTACTTTGCTATTGATATGTGTGTTGGAAGCTTGATATTGTTTATTTGGCAGAAGGTTGATAGGGCTAAGGCTGATGCATTTGGATCAGCTGTTGCGTCCGGTTTGATTTGTGGAGATGGAATTTGGTCACTTCCTAGCTCTTTTCTTGCTCTTGCTGGAGTGCAGCCACCTATTTGCATGAAATTCTTGTCAAGAGCAGCAAATGCTAAGGTTGATAAGTTCTTAGGATCTTGAAGTTGACTTTATGGATCTCAGACACAACAACCCGCAACAACTTCACAATCTGCTGCTTCTCAGATTCAAACAATGGAAAACAATTCACAACTTGGTGCACATTCAACATCTAGGAGGCAGCAAGCTCACAGAAGAAACAATCTTCAACCAGCTCATATATAGGCACAATGTAATTTAGAACCTTGTATTCTTTTATACCTTTTGATTGATGAATGTTTATGTAAATGAAAACCTATGTTATATACCTTTTGAATTGGTTTTGAGTCATGACTATGTTTTATAACCTTCTGAGTAATGATTATATCTTATAACATTTTGATGATATAACTTATATGAGATACGATTACAGTTGTGTTAAAATTATGCAAATTGTATCAACAAAGTGACAAACATAGGTAACACATTCACATTTGCAGTCATATAAACATTGATCTCATCTATTCAATAATTCATAGTCAAATGAATAGAGTAAAATGAGCATTGCTATTTGTCCCGATACACTTTATCCGAAACTGCTCCCCCGTAAAGGTTTATAACCAACTCAATGTCATCCAAACCGGAAAACAAGGAAAGTCGTGTCGTGTGTATCATTTAAGAATAATATTTGGCATATATACGGTGGTGAAATCTTCGCATCATAATCTCTCGCGATATCCAGCAATTCCCTTAAAACATATTGGTACATATATTTTGGACCAACTCAATTTCATCTTAAATAAACCCGGTTCAAACTTAGTCATAGGTTATCGGGTTTATCAACGGATATCAGTTAACATATCACACAAACTCACAGACACACTAAACCACAGAGAGAGTTGAGAGGATGGGAACAAATAATGCTATAACCAACTATCTAACAATGTAAGGAAAGAATTTGATTAAGAGAAATGACTTGACTGACAACAATAAGACAAGTATAAGAAGCCAGAGTTATGATAAGTTTTGCAATGAAAACTCCATGGTATGATATCATTTTTTTTTTTTTTAGATTTTTATTGTGCTTCAATTTCTCACGATGTTCCACATTGACAAAAAAAAAAGTTACTATGAATTAAATTTCAACTATTGAGCAAGTTATTTCATAATTTTGTTCCAAATAATAGTTATATAGAGCTTTAGCCATTAACCAACCAATAGTTTTCTGCAGTTTGATGTGAGAAAGAGTTCTGAAGAAGCAGATAAATCCGAAACAGAAAAATTTCTTAGTGGGTCTAACAAGTACAAAGCGGTACAGTATGATCTATTTATCTGTGGTTTTCTATATCTTAGCCATAAGAAATAGAAAAGATCAAACAAAAAAATTGCTCTATGATAGTGAACATTTAAGAAGATGCAGCGTTAGAATTCTATGAATTTCAGTTTGGAAGCTTTACTTGGTGAATTTCAGAAACCTTACGCCCCTGCTCTTTGCATGTTCGTCGGAAATGAGTATTTTTCTTTCAAAGATTCTGTATATCCTCCTCCGCATTTGATTTTCAATGCTTTTAACACTACTCCTTTTCATTCTGTTAAGGCTGTGATCCTTGGCCAGGTTTGTGGATTTCTGTTTGAATTTCAGTTTTCACTCTCTTTCTTTTAGTTCACTGATTTTGATTTTGGTTCTTTTAGGAACCTTATTCTGGACCTGGTGAAGCAATGGGGCTATCATTCTCGGCTCCCAAGGGAGTCAGATTTCCATCCATTTTGAAGAATGTACTTAAGGAGGTTAAGGATGATATTGGCTATAAGATTCTACGTCATGACAATCTTGAAAAATGGGCTCTGCAGGTAACCATTGATTAAAGCTTCTATAGAAATTGGTTATTTTGATATTGTGATCATTCTTTAAAGAATCTTGTCCAAACATTTCATTCTTAAAAATATTTATTTTATGCAAAAAGAGTGATTTCTTTGAATGGCCTCTCAATTTTTTGTAAACAAGAATCCATAACAAAATATACTAAAATGACGGATTTCAGACTAGTCATTCGCAATTAGAGCATACAGGGGTAGGGAACGCATTTGTGAACGCATTTATTTTATGTTTCCTTTTCTGTTATTCACTAATTTGTGCAGATGGTTTAACATTTGACTTAAGTAGTAGATTTTGTTTTTCAAAAAATATTCTCTGACTACAACTTTAACTTAATTTCAGGGCGTTCTCTTGCTGAATGTTGTTCTCACAGGTTAGATATTTTACATTTTGGTGAACTTCTAATCAAGAGTATTGGTGACTTAAAGTCATAAACTGTTTGTGCATGGTGATCTAGTACGAAAACAACCCTTACTATGTTTGTGTTTGGTGTATTATTGTTAATAACTAATAAGTATATATATTTATTCAAAAAAAAAAAAAAACTAATAAGTATATATATGTTATCATTAGGAATAAATTAATGAGGACACTAGGGAATGGTAGATGAAATAAAAAAATTATTTATTTAGATTTATTGAGGTTAGTGTACAAAAATAATTACTTATTTATTTAGATTTGTTGAGGAGGGTTGGATTATAGGCTAGTATAGAAATCTTGAGAGAAGATTGTTGTTGTGGTGAAAATAAATTGAGTTAACTATTTTAAGATGATGAAATCGATAGTGATTTTGTTCATTTATGATTTTTAAAATGATTTAGAATTCTACTCCTGTAGAAATGGCTCTGGTCAACAATTTTCTGACCAACTATCTGTTTTTCTTGACGTGGTCAGCTTGGTATTTTCTATCTTCAGGTTGTTGCATTTGAAGTTCAAATGACAAATCAAACATTAGAAGACACATTTGCTATGGTTGGACACACAACTTTCAAATACATATTTCCAGGAAGCTTAAAAGCTTAA
Protein sequence

>MS.gene049639.t1

MSREPYDIESHDTFAVAPVSKDEQREEISVEKGFEGTYVPMWQKQVTVRAIFVSLMLSVMFTFIVMKLNLTTGIMPSLNVSAGLLGFFFVKTWTKLLEKAGWLNQPFTRQENTVIQTCVVAASGIAFSGGFCSNLFGMSPTIAKQIPEFDTANDVKSLGLGWMIAFLFVVSFLGLFSVVPLRKIMIVDFKLTYPSGTATAHLINSFHTSEALGKFFSFSFLWGLFQWFFTAGDACGFANFPTFGLEAYQHKFYFDFSATYVGVGMICPYIINISLLIGGILSWGVMWPLIGAKKGDWFPADLKQSSFHGLQAYKVFIAIAMILGDGLYNFVKVLGTTLLGLYKQWKKKDKRAQPDDPNAPLPPTISFDNKRRTEMFLKDQIPSWFAIAGYVIIAIISTITIPHIFHQLKWYHVICIYIIAPALAFCNAYGCGLTDWSLAGTYGKLAIFAIGAWAREANGGVLAGLAACGVMMNIVSTASDLMQDFKTGYMTLASPKSMFVSQVIGTAMGCVISPCVFWIFYNAFGTLGQPGTAYPAPYALVYRNIAILGVNGFSAFPKNCLVLCIVFFIGAMIINAVRDWAGKKYVNFIPVPMAMAIPFYIGSYFAIDMCVGSLILFIWQKVDRAKADAFGSAVASGLICGDGIWSLPSSFLALAGVQPPICMKFLSRAANAKFDVRKSSEEADKSETEKFLSGSNKYKAKPYAPALCMFVGNEYFSFKDSVYPPPHLIFNAFNTTPFHSVKAVILGQEPYSGPGEAMGLSFSAPKGVRFPSILKNVLKEVKDDIGYKILRHDNLEKWALQVVAFEVQMTNQTLEDTFAMVGHTTFKYIFPGSLKA