Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049650.t1 | XP_003600586.1 | 97.2 | 71 | 2 | 0 | 1 | 71 | 1 | 71 | 8.30E-30 | 139 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049650.t1 | G7J780 | 97.2 | 71 | 2 | 0 | 1 | 71 | 1 | 71 | 6.0e-30 | 139.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049650 | MS.gene049653 | 0.805521 | 1.28E-49 | -1.69E-46 |
| MS.gene049650 | MS.gene067635 | 0.813058 | 3.10E-51 | -1.69E-46 |
| MS.gene049650 | MS.gene071975 | 0.800538 | 1.37E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049650.t1 | MTR_3g063970 | 97.183 | 71 | 2 | 0 | 1 | 71 | 1 | 71 | 1.17e-45 | 140 |
| MS.gene049650.t1 | MTR_3g063970 | 95.833 | 48 | 2 | 0 | 1 | 48 | 1 | 48 | 2.71e-15 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049650.t1 | AT3G14430 | 66.667 | 63 | 20 | 1 | 9 | 71 | 15 | 76 | 2.46e-25 | 89.4 |
Find 17 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGCATCAATTCCTTTAAA+AGG | 0.176489 | 3.2:-59513993 | None:intergenic |
| CTTTAAAAGGGCCAAAGTTC+TGG | 0.296338 | 3.2:-59513980 | None:intergenic |
| ATTTCTTACGAAGGCGAAAA+TGG | 0.322719 | 3.2:-59512986 | None:intergenic |
| ACAAATACTTGATTGTATTC+TGG | 0.329433 | 3.2:-59513938 | None:intergenic |
| AAGCATCAATTCCTTTAAAA+GGG | 0.454386 | 3.2:-59513992 | None:intergenic |
| CTGGTGGTGGACCAGAACTT+TGG | 0.486503 | 3.2:+59513969 | MS.gene049650:CDS |
| CAAAGTTCTGGTCCACCACC+AGG | 0.502762 | 3.2:-59513968 | None:intergenic |
| CAAGTATTTGTGAAGAAGCC+TGG | 0.508253 | 3.2:+59513950 | MS.gene049650:CDS |
| TGAAGAAGAAAAGAAAGAAG+AGG | 0.526246 | 3.2:-59513023 | None:intergenic |
| ACCGATGGCTAGGCGGCGAG+TGG | 0.540079 | 3.2:+59512924 | None:intergenic |
| GAGAGAGAACACCGATGGCT+AGG | 0.547993 | 3.2:+59512914 | None:intergenic |
| CTAGGCGGCGAGTGGAGACT+CGG | 0.556687 | 3.2:+59512932 | MS.gene049650:CDS |
| TTTGTGAAGAAGCCTGGTGG+TGG | 0.604245 | 3.2:+59513956 | MS.gene049650:CDS |
| GGAACGAGTCGTTATTAACT+CGG | 0.605723 | 3.2:+59512953 | MS.gene049650:CDS |
| AAACTCAGCATTTCTTACGA+AGG | 0.638252 | 3.2:-59512995 | None:intergenic |
| AGAGAACACCGATGGCTAGG+CGG | 0.664882 | 3.2:+59512917 | None:intergenic |
| GTATTTGTGAAGAAGCCTGG+TGG | 0.787083 | 3.2:+59513953 | MS.gene049650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCATTTTATCAAATTATTAT+TGG | - | chr3.2:59513660-59513679 | None:intergenic | 10.0% |
| !! | ATAATTCATTCATATATGGT+TGG | + | chr3.2:59513781-59513800 | MS.gene049650:intron | 20.0% |
| !! | ATTTGAACAAATGATGTAAA+TGG | - | chr3.2:59513127-59513146 | None:intergenic | 20.0% |
| !! | CTTCATAATTCATTCATATA+TGG | + | chr3.2:59513777-59513796 | MS.gene049650:intron | 20.0% |
| !! | GATTGGATAATTATATCAAA+AGG | + | chr3.2:59513274-59513293 | MS.gene049650:intron | 20.0% |
| !! | TTTGAACAAATGATGTAAAT+GGG | - | chr3.2:59513126-59513145 | None:intergenic | 20.0% |
| !!! | AATTTTCGTTCTTTTTTATG+TGG | + | chr3.2:59513144-59513163 | MS.gene049650:intron | 20.0% |
| !!! | ATATTCACAAGTTTTTGTAT+CGG | + | chr3.2:59513492-59513511 | MS.gene049650:intron | 20.0% |
| !!! | TTTTGATAAGTGGAATTTTT+TGG | + | chr3.2:59513804-59513823 | MS.gene049650:intron | 20.0% |
| ! | AAGAAAAAGAAAAAAGAGAG+AGG | - | chr3.2:59513086-59513105 | None:intergenic | 25.0% |
| ! | ACAAATACTTGATTGTATTC+TGG | - | chr3.2:59513941-59513960 | None:intergenic | 25.0% |
| ! | ACATAATTCAGTGTAATCAT+AGG | + | chr3.2:59513679-59513698 | MS.gene049650:intron | 25.0% |
| ! | CAAAATTACAAGACAAACAA+AGG | - | chr3.2:59513880-59513899 | None:intergenic | 25.0% |
| ! | TAAATTGAAAACTGCATCAT+TGG | + | chr3.2:59513241-59513260 | MS.gene049650:intron | 25.0% |
| ! | TATCAAATTATTATTGGCGT+CGG | - | chr3.2:59513654-59513673 | None:intergenic | 25.0% |
| ! | TATGAATGAATTATGAAGCA+TGG | - | chr3.2:59513775-59513794 | None:intergenic | 25.0% |
| !! | TTGTCTTGTAATTTTGCTTT+TGG | + | chr3.2:59513884-59513903 | MS.gene049650:intron | 25.0% |
| AATTACAAGACAAACAAAGG+AGG | - | chr3.2:59513877-59513896 | None:intergenic | 30.0% | |
| ATTACAAGACAAACAAAGGA+GGG | - | chr3.2:59513876-59513895 | None:intergenic | 30.0% | |
| TGAAGAAGAAAAGAAAGAAG+AGG | - | chr3.2:59513026-59513045 | None:intergenic | 30.0% | |
| !! | TCATTGGTTTGATGATTGAT+TGG | + | chr3.2:59513257-59513276 | MS.gene049650:intron | 30.0% |
| !!! | ATATGGTTGGTTTTGATAAG+TGG | + | chr3.2:59513794-59513813 | MS.gene049650:intron | 30.0% |
| AAACTCAGCATTTCTTACGA+AGG | - | chr3.2:59512998-59513017 | None:intergenic | 35.0% | |
| AACTATATACCAGTTCCAAC+TGG | - | chr3.2:59513314-59513333 | None:intergenic | 35.0% | |
| ACATGAAAAGAGAAACACGA+TGG | - | chr3.2:59513170-59513189 | None:intergenic | 35.0% | |
| ACTGATGAAAACAACTTACG+AGG | - | chr3.2:59513462-59513481 | None:intergenic | 35.0% | |
| ATTTCTTACGAAGGCGAAAA+TGG | - | chr3.2:59512989-59513008 | None:intergenic | 35.0% | |
| CATGTATCATGTATGAAGCA+CGG | + | chr3.2:59513587-59513606 | MS.gene049650:intron | 35.0% | |
| TTCAGTGTAATCATAGGTGT+CGG | + | chr3.2:59513685-59513704 | MS.gene049650:intron | 35.0% | |
| ATTCTGATTCCAGTTGGAAC+TGG | + | chr3.2:59513302-59513321 | MS.gene049650:intron | 40.0% | |
| CAAGTATTTGTGAAGAAGCC+TGG | + | chr3.2:59513950-59513969 | MS.gene049650:CDS | 40.0% | |
| CGTGCTTCATACATGATACA+TGG | - | chr3.2:59513589-59513608 | None:intergenic | 40.0% | |
| GGAACGAGTCGTTATTAACT+CGG | + | chr3.2:59512953-59512972 | MS.gene049650:CDS | 40.0% | |
| GTAGAGATTCTGATTCCAGT+TGG | + | chr3.2:59513296-59513315 | MS.gene049650:intron | 40.0% | |
| TTCTCAGATTAGGTGTGTCA+TGG | - | chr3.2:59513748-59513767 | None:intergenic | 40.0% | |
| ACAAACAAAGGAGGGAGTAG+GGG | - | chr3.2:59513868-59513887 | None:intergenic | 45.0% | |
| AGACAAACAAAGGAGGGAGT+AGG | - | chr3.2:59513870-59513889 | None:intergenic | 45.0% | |
| GACAAACAAAGGAGGGAGTA+GGG | - | chr3.2:59513869-59513888 | None:intergenic | 45.0% | |
| GCATGGACACTTCTCAGATT+AGG | - | chr3.2:59513758-59513777 | None:intergenic | 45.0% | |
| GTATTTGTGAAGAAGCCTGG+TGG | + | chr3.2:59513953-59513972 | MS.gene049650:CDS | 45.0% | |
| !! | ATTCTGGTCTTGATGTCTGC+AGG | - | chr3.2:59513925-59513944 | None:intergenic | 45.0% |
| CAAACAAAGGAGGGAGTAGG+GGG | - | chr3.2:59513867-59513886 | None:intergenic | 50.0% | |
| CATGGACATCAGACACGACA+CGG | + | chr3.2:59513617-59513636 | MS.gene049650:intron | 50.0% | |
| TCATGTATGAAGCACGGACG+CGG | + | chr3.2:59513593-59513612 | MS.gene049650:intron | 50.0% | |
| TTTGTGAAGAAGCCTGGTGG+TGG | + | chr3.2:59513956-59513975 | MS.gene049650:CDS | 50.0% | |
| !! | CATAGGTGTCGGTGTCGTTT+CGG | + | chr3.2:59513696-59513715 | MS.gene049650:intron | 50.0% |
| !! | TCATGGTGTCGTACACGCAT+TGG | - | chr3.2:59513731-59513750 | None:intergenic | 50.0% |
| ! | CAAAGTTCTGGTCCACCACC+AGG | - | chr3.2:59513971-59513990 | None:intergenic | 55.0% |
| !! | CTGGTGGTGGACCAGAACTT+TGG | + | chr3.2:59513969-59513988 | MS.gene049650:CDS | 55.0% |
| !! | TGTCGGTGTCGTTTCGGTGT+CGG | + | chr3.2:59513702-59513721 | MS.gene049650:intron | 55.0% |
| ACAAAGGAGGGAGTAGGGGG+TGG | - | chr3.2:59513864-59513883 | None:intergenic | 60.0% | |
| ATGAAGCACGGACGCGGACA+TGG | + | chr3.2:59513599-59513618 | MS.gene049650:intron | 60.0% | |
| AGGAGGGAGTAGGGGGTGGA+AGG | - | chr3.2:59513860-59513879 | None:intergenic | 65.0% | |
| CTAGGCGGCGAGTGGAGACT+CGG | + | chr3.2:59512932-59512951 | MS.gene049650:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 59512928 | 59514000 | 59512928 | ID=MS.gene049650 |
| chr3.2 | mRNA | 59512928 | 59514000 | 59512928 | ID=MS.gene049650.t1;Parent=MS.gene049650 |
| chr3.2 | exon | 59512928 | 59513071 | 59512928 | ID=MS.gene049650.t1.exon1;Parent=MS.gene049650.t1 |
| chr3.2 | CDS | 59512928 | 59513071 | 59512928 | ID=cds.MS.gene049650.t1;Parent=MS.gene049650.t1 |
| chr3.2 | exon | 59513929 | 59514000 | 59513929 | ID=MS.gene049650.t1.exon2;Parent=MS.gene049650.t1 |
| chr3.2 | CDS | 59513929 | 59514000 | 59513929 | ID=cds.MS.gene049650.t1;Parent=MS.gene049650.t1 |
| Gene Sequence |
| Protein sequence |
