AlfalfaGEDB | MS.gene071975

M. sativa cultivar XinJiangDaYe / MS.gene071975

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071975.t1	AES63364.1	93.9	49	3	0	1	49	25	73	1.70E-18	100.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071975.t1	G7IL76	93.9	49	3	0	1	49	25	73	1.2e-18	100.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene071975	0.813029	3.15E-51	-1.69E-46
MS.gene049293	MS.gene071975	-0.824901	6.28E-54	-1.69E-46
MS.gene049419	MS.gene071975	-0.81915	1.35E-52	-1.69E-46
MS.gene049650	MS.gene071975	0.800538	1.37E-48	-1.69E-46
MS.gene049653	MS.gene071975	0.827438	1.56E-54	-1.69E-46
MS.gene049680	MS.gene071975	0.816227	6.16E-52	-1.69E-46
MS.gene049863	MS.gene071975	0.81478	1.29E-51	-1.69E-46
MS.gene049951	MS.gene071975	0.806348	8.59E-50	-1.69E-46
MS.gene050288	MS.gene071975	0.800408	1.46E-48	-1.69E-46
MS.gene050320	MS.gene071975	0.804611	1.99E-49	-1.69E-46
MS.gene050646	MS.gene071975	0.8101	1.36E-50	-1.69E-46
MS.gene050752	MS.gene071975	0.868853	4.79E-66	-1.69E-46
MS.gene050945	MS.gene071975	0.838485	2.80E-57	-1.69E-46
MS.gene050955	MS.gene071975	0.867851	1.01E-65	-1.69E-46
MS.gene051003	MS.gene071975	0.810384	1.18E-50	-1.69E-46
MS.gene051043	MS.gene071975	0.816994	4.15E-52	-1.69E-46
MS.gene051234	MS.gene071975	0.810586	1.07E-50	-1.69E-46
MS.gene051343	MS.gene071975	0.830111	3.53E-55	-1.69E-46
MS.gene051597	MS.gene071975	0.801659	8.11E-49	-1.69E-46
MS.gene051676	MS.gene071975	0.823072	1.69E-53	-1.69E-46
MS.gene051748	MS.gene071975	0.807556	4.77E-50	-1.69E-46
MS.gene051832	MS.gene071975	0.812704	3.71E-51	-1.69E-46
MS.gene05188	MS.gene071975	0.819133	1.36E-52	-1.69E-46
MS.gene051902	MS.gene071975	0.83254	8.92E-56	-1.69E-46
MS.gene051903	MS.gene071975	0.811603	6.44E-51	-1.69E-46
MS.gene051975	MS.gene071975	0.858745	6.67E-63	-1.69E-46
MS.gene052072	MS.gene071975	0.828676	7.88E-55	-1.69E-46
MS.gene052242	MS.gene071975	-0.80857	2.90E-50	-1.69E-46
MS.gene052479	MS.gene071975	0.803953	2.73E-49	-1.69E-46
MS.gene052947	MS.gene071975	0.821106	4.82E-53	-1.69E-46
MS.gene053058	MS.gene071975	0.813137	2.98E-51	-1.69E-46
MS.gene053109	MS.gene071975	0.806527	7.88E-50	-1.69E-46
MS.gene053189	MS.gene071975	0.800541	1.37E-48	-1.69E-46
MS.gene053340	MS.gene071975	0.801012	1.10E-48	-1.69E-46
MS.gene053564	MS.gene071975	0.825928	3.59E-54	-1.69E-46
MS.gene053931	MS.gene071975	0.808095	3.66E-50	-1.69E-46
MS.gene053963	MS.gene071975	0.828436	9.00E-55	-1.69E-46
MS.gene054222	MS.gene071975	0.814223	1.72E-51	-1.69E-46
MS.gene054403	MS.gene071975	0.871815	5.13E-67	-1.69E-46
MS.gene054413	MS.gene071975	0.803185	3.93E-49	-1.69E-46
MS.gene05469	MS.gene071975	0.87071	1.19E-66	-1.69E-46
MS.gene054946	MS.gene071975	-0.816801	4.59E-52	-1.69E-46
MS.gene05548	MS.gene071975	0.800566	1.36E-48	-1.69E-46
MS.gene055555	MS.gene071975	0.827089	1.90E-54	-1.69E-46
MS.gene055841	MS.gene071975	0.801136	1.04E-48	-1.69E-46
MS.gene056631	MS.gene071975	0.818242	2.17E-52	-1.69E-46
MS.gene056986	MS.gene071975	0.86312	3.12E-64	-1.69E-46
MS.gene057102	MS.gene071975	0.832863	7.41E-56	-1.69E-46
MS.gene057208	MS.gene071975	0.802106	6.57E-49	-1.69E-46
MS.gene057694	MS.gene071975	0.820856	5.50E-53	-1.69E-46
MS.gene057705	MS.gene071975	0.856311	3.51E-62	-1.69E-46
MS.gene057890	MS.gene071975	-0.80119	1.01E-48	-1.69E-46
MS.gene057999	MS.gene071975	0.859953	2.89E-63	-1.69E-46
MS.gene058074	MS.gene071975	0.831625	1.50E-55	-1.69E-46
MS.gene058238	MS.gene071975	0.827285	1.70E-54	-1.69E-46
MS.gene059146	MS.gene071975	0.818094	2.34E-52	-1.69E-46
MS.gene059317	MS.gene071975	0.80007	1.71E-48	-1.69E-46
MS.gene05985	MS.gene071975	-0.838571	2.66E-57	-1.69E-46
MS.gene06011	MS.gene071975	0.843056	1.77E-58	-1.69E-46
MS.gene060244	MS.gene071975	0.826788	2.24E-54	-1.69E-46
MS.gene060365	MS.gene071975	0.840295	9.48E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071975.t1	MTR_2g007950	93.878	49	3	0	1	49	25	73	1.92e-28	95.9

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071975.t1	AT1G25275	71.053	38	11	10	47	34	71	6.70e-14	59.7
MS.gene071975.t1	AT1G25275	71.053	38	11	10	47	34	71	1.31e-13	58.5
MS.gene071975.t1	AT1G25275	68.421	38	12	10	47	34	71	1.10e-12	56.2

Find 12 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGCTTCTGATTGCCTTGA+TGG	0.154913	2.4:+74993678	MS.gene071975:CDS
CTCTTTGAACGCAGGCAGTT+TGG	0.316423	2.4:-74993704	None:intergenic
TGCTCGATTAGATGCGGTCC+AGG	0.477134	2.4:+74993943	MS.gene071975:CDS
TGCAGATTCTATGGTGGAGA+AGG	0.494670	2.4:+74994207	MS.gene071975:intron
TCAAGGCAATCAGAAGCATC+AGG	0.516390	2.4:-74993675	None:intergenic
GTGACATGCAGCAAGGCTTA+CGG	0.519988	2.4:+74993903	MS.gene071975:intron
GCAGATTCTATGGTGGAGAA+GGG	0.551697	2.4:+74994208	MS.gene071975:intron
AGGCAGTTTGGCAACCATCA+AGG	0.564306	2.4:-74993692	None:intergenic
TTCTATGGTGGAGAAGGGCA+TGG	0.592128	2.4:+74994213	MS.gene071975:CDS
ATGCAGCAAGGCTTACGGCA+AGG	0.642528	2.4:+74993908	MS.gene071975:intron
CGCAAGTGCTCGATTAGATG+CGG	0.643345	2.4:+74993937	MS.gene071975:CDS
CTAACAGTCTCTTTGAACGC+AGG	0.676017	2.4:-74993712	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTAAGAAAAGTAATTAAA+AGG	-	chr2.4:74993758-74993777	None:intergenic	10.0%
!!!	AATAATACTATTTTAATGAA+AGG	+	chr2.4:74993983-74994002	MS.gene071975:intron	10.0%
!!!	CATTAAAATAGTATTATTTT+GGG	-	chr2.4:74993981-74994000	None:intergenic	10.0%
!!!	TCATTAAAATAGTATTATTT+TGG	-	chr2.4:74993982-74994001	None:intergenic	10.0%
!!	AAATTAATTAAAAAGCTTCA+AGG	+	chr2.4:74993857-74993876	MS.gene071975:intron	15.0%
!!!	GATTTTCTTCTTTTAATTTA+AGG	+	chr2.4:74993778-74993797	MS.gene071975:intron	15.0%
!!	CAAGAAATTTAATTAAACCT+CGG	-	chr2.4:74994051-74994070	None:intergenic	20.0%
!!!	ATTTAATTTTGCAGATTCTA+TGG	+	chr2.4:74994198-74994217	MS.gene071975:intron	20.0%
	CTTGAATAATCGTAAACAAC+AGG	+	chr2.4:74994067-74994086	MS.gene071975:intron	30.0%
	TTTGGGTAATGTTAATTACC+TGG	-	chr2.4:74993964-74993983	None:intergenic	30.0%
!	TAATTTTGCAGATTCTATGG+TGG	+	chr2.4:74994201-74994220	MS.gene071975:intron	30.0%
	ATAATCGTAAACAACAGGTC+TGG	+	chr2.4:74994072-74994091	MS.gene071975:intron	35.0%
	TAATCGTAAACAACAGGTCT+GGG	+	chr2.4:74994073-74994092	MS.gene071975:intron	35.0%
	GTATAAAATTGTCTGAGCCC+AGG	-	chr2.4:74994152-74994171	None:intergenic	40.0%
	TATGTATGTGACATGCAGCA+AGG	+	chr2.4:74993896-74993915	MS.gene071975:intron	40.0%
!	TTAGTGCTGAAAAGATACCG+AGG	+	chr2.4:74994031-74994050	MS.gene071975:intron	40.0%
	CTAACAGTCTCTTTGAACGC+AGG	-	chr2.4:74993715-74993734	None:intergenic	45.0%
	GATGCTTCTGATTGCCTTGA+TGG	+	chr2.4:74993678-74993697	MS.gene071975:CDS	45.0%
!	GCAGATTCTATGGTGGAGAA+GGG	+	chr2.4:74994208-74994227	MS.gene071975:intron	45.0%
!	TGCAGATTCTATGGTGGAGA+AGG	+	chr2.4:74994207-74994226	MS.gene071975:intron	45.0%
!!	TCAAGGCAATCAGAAGCATC+AGG	-	chr2.4:74993678-74993697	None:intergenic	45.0%
	AGGCAGTTTGGCAACCATCA+AGG	-	chr2.4:74993695-74993714	None:intergenic	50.0%
	CGCAAGTGCTCGATTAGATG+CGG	+	chr2.4:74993937-74993956	MS.gene071975:CDS	50.0%
	CTCTTTGAACGCAGGCAGTT+TGG	-	chr2.4:74993707-74993726	None:intergenic	50.0%
	GTGACATGCAGCAAGGCTTA+CGG	+	chr2.4:74993903-74993922	MS.gene071975:intron	50.0%
	TCGTAAACAACAGGTCTGGG+TGG	+	chr2.4:74994076-74994095	MS.gene071975:intron	50.0%
!	ACGCTAGTCTCACACACTAG+AGG	+	chr2.4:74994113-74994132	MS.gene071975:intron	50.0%
!	TTCTATGGTGGAGAAGGGCA+TGG	+	chr2.4:74994213-74994232	MS.gene071975:CDS	50.0%
	ACAGGTCTGGGTGGTGTAGT+CGG	+	chr2.4:74994085-74994104	MS.gene071975:intron	55.0%
	ATGCAGCAAGGCTTACGGCA+AGG	+	chr2.4:74993908-74993927	MS.gene071975:intron	55.0%
	TCTGAGCCCAGGTTCGAACT+GGG	-	chr2.4:74994141-74994160	None:intergenic	55.0%
	TGCTCGATTAGATGCGGTCC+AGG	+	chr2.4:74993943-74993962	MS.gene071975:CDS	55.0%
	AGAGGTCCCCAGTTCGAACC+TGG	+	chr2.4:74994131-74994150	MS.gene071975:intron	60.0%
	CTGAGCCCAGGTTCGAACTG+GGG	-	chr2.4:74994140-74994159	None:intergenic	60.0%
	GAGGTCCCCAGTTCGAACCT+GGG	+	chr2.4:74994132-74994151	MS.gene071975:intron	60.0%
	GTCTGAGCCCAGGTTCGAAC+TGG	-	chr2.4:74994142-74994161	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	74993660	74994240	74993660	ID=MS.gene071975
chr2.4	mRNA	74993660	74994240	74993660	ID=MS.gene071975.t1;Parent=MS.gene071975
chr2.4	exon	74993660	74993729	74993660	ID=MS.gene071975.t1.exon1;Parent=MS.gene071975.t1
chr2.4	CDS	74993660	74993729	74993660	ID=cds.MS.gene071975.t1;Parent=MS.gene071975.t1
chr2.4	exon	74993914	74993964	74993914	ID=MS.gene071975.t1.exon2;Parent=MS.gene071975.t1
chr2.4	CDS	74993914	74993964	74993914	ID=cds.MS.gene071975.t1;Parent=MS.gene071975.t1
chr2.4	exon	74994212	74994240	74994212	ID=MS.gene071975.t1.exon3;Parent=MS.gene071975.t1
chr2.4	CDS	74994212	74994240	74994212	ID=cds.MS.gene071975.t1;Parent=MS.gene071975.t1

Gene Sequence

>MS.gene071975

ATGGATAGCGTTGTTCCTGATGCTTCTGATTGCCTTGATGGTTGCCAAACTGCCTGCGTTCAAAGAGACTGTTAGTCACTCTTCTCAATCATCATCCTTTTAATTACTTTTCTTAATTGATTTTCTTCTTTTAATTTAAGGATTCATTATTGACTCTTACTTATATTGTATGCATAAAAACTTCAATGATGTTTATCAAATTAATTAAAAAGCTTCAAGGATTTGAGACTTAAATATATGTATGTGACATGCAGCAAGGCTTACGGCAAGGTGTGAGCGCAAGTGCTCGATTAGATGCGGTCCAGGTAATTAACATTACCCAAAATAATACTATTTTAATGAAAGGATTATTTCTGTCAAAAAATAATAATTTAGTGCTGAAAAGATACCGAGGTTTAATTAAATTTCTTGAATAATCGTAAACAACAGGTCTGGGTGGTGTAGTCGGTTATCACGCTAGTCTCACACACTAGAGGTCCCCAGTTCGAACCTGGGCTCAGACAATTTTATACATTTTTTTTATGTATTTTTTCTCTGTATTTAATTTTGCAGATTCTATGGTGGAGAAGGGCATGGATTGA

Protein sequence

>MS.gene071975.t1

MDSVVPDASDCLDGCQTACVQRDSRLTARCERKCSIRCGPDSMVEKGMD