AlfalfaGEDB | MS.gene049653

M. sativa cultivar XinJiangDaYe / MS.gene049653

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049653.t1	XP_003600586.1	98.6	71	1	0	1	71	1	71	2.80E-30	140.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049653.t1	G7J780	98.6	71	1	0	1	71	1	71	2.1e-30	140.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049424	MS.gene049653	0.823376	1.43E-53	-1.69E-46
MS.gene049650	MS.gene049653	0.805521	1.28E-49	-1.69E-46
MS.gene049653	MS.gene049951	0.809656	1.70E-50	-1.69E-46
MS.gene049653	MS.gene050277	0.820246	7.59E-53	-1.69E-46
MS.gene049653	MS.gene050689	0.813642	2.31E-51	-1.69E-46
MS.gene049653	MS.gene050752	0.801128	1.04E-48	-1.69E-46
MS.gene049653	MS.gene051243	0.801775	7.68E-49	-1.69E-46
MS.gene049653	MS.gene052286	0.825224	5.27E-54	-1.69E-46
MS.gene049653	MS.gene052954	0.812944	3.28E-51	-1.69E-46
MS.gene049653	MS.gene053006	0.812349	4.43E-51	-1.69E-46
MS.gene049653	MS.gene053590	0.814068	1.86E-51	-1.69E-46
MS.gene049653	MS.gene055841	0.822323	2.52E-53	-1.69E-46
MS.gene049653	MS.gene056760	0.805794	1.12E-49	-1.69E-46
MS.gene049653	MS.gene057140	0.800144	1.65E-48	-1.69E-46
MS.gene049653	MS.gene057234	0.804282	2.33E-49	-1.69E-46
MS.gene049653	MS.gene057392	0.837159	6.13E-57	-1.69E-46
MS.gene049653	MS.gene057705	0.805919	1.06E-49	-1.69E-46
MS.gene049653	MS.gene05795	0.820158	7.95E-53	-1.69E-46
MS.gene049653	MS.gene058008	0.816306	5.92E-52	-1.69E-46
MS.gene049653	MS.gene058009	0.817355	3.44E-52	-1.69E-46
MS.gene049653	MS.gene058055	0.804324	2.28E-49	-1.69E-46
MS.gene049653	MS.gene058238	0.800329	1.52E-48	-1.69E-46
MS.gene049653	MS.gene059317	0.828681	7.85E-55	-1.69E-46
MS.gene049653	MS.gene062501	0.830864	2.31E-55	-1.69E-46
MS.gene049653	MS.gene063288	0.815869	7.41E-52	-1.69E-46
MS.gene049653	MS.gene064937	0.813742	2.19E-51	-1.69E-46
MS.gene049653	MS.gene066067	0.842173	3.04E-58	-1.69E-46
MS.gene049653	MS.gene06667	0.827831	1.26E-54	-1.69E-46
MS.gene049653	MS.gene067635	0.82605	3.36E-54	-1.69E-46
MS.gene049653	MS.gene06797	0.800745	1.25E-48	-1.69E-46
MS.gene049653	MS.gene068166	0.8317	1.44E-55	-1.69E-46
MS.gene049653	MS.gene06836	0.806608	7.57E-50	-1.69E-46
MS.gene049653	MS.gene06883	0.817522	3.16E-52	-1.69E-46
MS.gene049653	MS.gene069828	0.801546	8.56E-49	-1.69E-46
MS.gene049653	MS.gene06989	0.819479	1.14E-52	-1.69E-46
MS.gene049653	MS.gene07090	0.821966	3.05E-53	-1.69E-46
MS.gene049653	MS.gene07100	0.814195	1.74E-51	-1.69E-46
MS.gene049653	MS.gene071074	0.816145	6.43E-52	-1.69E-46
MS.gene049653	MS.gene071076	0.806329	8.67E-50	-1.69E-46
MS.gene049653	MS.gene071975	0.827438	1.56E-54	-1.69E-46
MS.gene049653	MS.gene07217	0.816395	5.65E-52	-1.69E-46
MS.gene049653	MS.gene073003	0.826785	2.24E-54	-1.69E-46
MS.gene049653	MS.gene073564	0.807184	5.72E-50	-1.69E-46
MS.gene049653	MS.gene073674	0.829961	3.84E-55	-1.69E-46
MS.gene049653	MS.gene073954	0.817394	3.37E-52	-1.69E-46
MS.gene049653	MS.gene08649	0.808389	3.17E-50	-1.69E-46
MS.gene049653	MS.gene08724	0.805954	1.04E-49	-1.69E-46
MS.gene049653	MS.gene09061	0.80423	2.39E-49	-1.69E-46
MS.gene049653	MS.gene09850	0.811914	5.52E-51	-1.69E-46
MS.gene049653	MS.gene20251	0.805703	1.17E-49	-1.69E-46
MS.gene049653	MS.gene20430	0.813369	2.65E-51	-1.69E-46
MS.gene049653	MS.gene21490	0.803144	4.01E-49	-1.69E-46
MS.gene049653	MS.gene22314	0.810496	1.12E-50	-1.69E-46
MS.gene049653	MS.gene22968	0.819288	1.26E-52	-1.69E-46
MS.gene049653	MS.gene23954	0.802133	6.48E-49	-1.69E-46
MS.gene049653	MS.gene25475	0.818917	1.53E-52	-1.69E-46
MS.gene049653	MS.gene26552	0.809072	2.27E-50	-1.69E-46
MS.gene049653	MS.gene28943	0.823616	1.26E-53	-1.69E-46
MS.gene049653	MS.gene29492	0.830078	3.60E-55	-1.69E-46
MS.gene049653	MS.gene31503	0.803122	4.05E-49	-1.69E-46
MS.gene049653	MS.gene31802	0.803215	3.88E-49	-1.69E-46
MS.gene049653	MS.gene35214	0.801924	7.16E-49	-1.69E-46
MS.gene049653	MS.gene35826	0.818038	2.42E-52	-1.69E-46
MS.gene049653	MS.gene39882	0.818448	1.95E-52	-1.69E-46
MS.gene049653	MS.gene42302	0.809757	1.62E-50	-1.69E-46
MS.gene049653	MS.gene43491	0.812956	3.27E-51	-1.69E-46
MS.gene049653	MS.gene43602	0.811192	7.92E-51	-1.69E-46
MS.gene049653	MS.gene45416	0.806721	7.17E-50	-1.69E-46
MS.gene049653	MS.gene45652	0.822918	1.83E-53	-1.69E-46
MS.gene049653	MS.gene46355	0.804538	2.06E-49	-1.69E-46
MS.gene049653	MS.gene47674	0.807932	3.97E-50	-1.69E-46
MS.gene049653	MS.gene47995	0.801494	8.77E-49	-1.69E-46
MS.gene049653	MS.gene49486	0.800663	1.30E-48	-1.69E-46
MS.gene049653	MS.gene50528	0.802551	5.32E-49	-1.69E-46
MS.gene049653	MS.gene50775	0.803481	3.41E-49	-1.69E-46
MS.gene049653	MS.gene52075	0.806943	6.44E-50	-1.69E-46
MS.gene049653	MS.gene53391	0.816291	5.97E-52	-1.69E-46
MS.gene049653	MS.gene53935	0.828631	8.08E-55	-1.69E-46
MS.gene049653	MS.gene55699	0.816663	4.92E-52	-1.69E-46
MS.gene049653	MS.gene55707	0.817797	2.74E-52	-1.69E-46
MS.gene049653	MS.gene56451	0.820735	5.86E-53	-1.69E-46
MS.gene049653	MS.gene56656	0.81858	1.82E-52	-1.69E-46
MS.gene049653	MS.gene57765	0.813078	3.07E-51	-1.69E-46
MS.gene049653	MS.gene57766	0.814005	1.92E-51	-1.69E-46
MS.gene049653	MS.gene57768	0.823885	1.09E-53	-1.69E-46
MS.gene049653	MS.gene57894	0.820083	8.27E-53	-1.69E-46
MS.gene049653	MS.gene59215	0.806089	9.74E-50	-1.69E-46
MS.gene049653	MS.gene59505	0.807648	4.56E-50	-1.69E-46
MS.gene049653	MS.gene59616	0.800022	1.75E-48	-1.69E-46
MS.gene049653	MS.gene60399	0.811694	6.16E-51	-1.69E-46
MS.gene049653	MS.gene61239	0.805353	1.39E-49	-1.69E-46
MS.gene049653	MS.gene61543	0.801408	9.13E-49	-1.69E-46
MS.gene049653	MS.gene63673	0.851791	7.05E-61	-1.69E-46
MS.gene049653	MS.gene63701	0.810322	1.22E-50	-1.69E-46
MS.gene049653	MS.gene64243	0.813848	2.08E-51	-1.69E-46
MS.gene049653	MS.gene64674	0.826735	2.30E-54	-1.69E-46
MS.gene049653	MS.gene64806	0.807411	5.12E-50	-1.69E-46
MS.gene049653	MS.gene67346	0.805236	1.47E-49	-1.69E-46
MS.gene049653	MS.gene68136	0.820093	8.23E-53	-1.69E-46
MS.gene049653	MS.gene68735	0.813549	2.42E-51	-1.69E-46
MS.gene049653	MS.gene69477	0.815981	6.99E-52	-1.69E-46
MS.gene049653	MS.gene69854	0.819577	1.08E-52	-1.69E-46
MS.gene049653	MS.gene71994	0.802744	4.85E-49	-1.69E-46
MS.gene049653	MS.gene73270	0.821556	3.79E-53	-1.69E-46
MS.gene049653	MS.gene75680	0.848559	5.67E-60	-1.69E-46
MS.gene049653	MS.gene75923	0.8268	2.22E-54	-1.69E-46
MS.gene049653	MS.gene76494	0.800219	1.60E-48	-1.69E-46
MS.gene049653	MS.gene79572	0.801512	8.69E-49	-1.69E-46
MS.gene049653	MS.gene79884	0.806754	7.05E-50	-1.69E-46
MS.gene049653	MS.gene82881	0.817544	3.12E-52	-1.69E-46
MS.gene049653	MS.gene85109	0.802571	5.27E-49	-1.69E-46
MS.gene049653	MS.gene85663	0.805821	1.11E-49	-1.69E-46
MS.gene049653	MS.gene86392	0.80383	2.89E-49	-1.69E-46
MS.gene049653	MS.gene88148	0.823814	1.13E-53	-1.69E-46
MS.gene049653	MS.gene89511	0.800639	1.31E-48	-1.69E-46
MS.gene049653	MS.gene89677	0.800166	1.64E-48	-1.69E-46
MS.gene049653	MS.gene90981	0.814109	1.82E-51	-1.69E-46
MS.gene049653	MS.gene92135	0.803133	4.03E-49	-1.69E-46
MS.gene049653	MS.gene92557	0.80608	9.78E-50	-1.69E-46
MS.gene049653	MS.gene93875	0.805147	1.54E-49	-1.69E-46
MS.gene049653	MS.gene95039	0.810006	1.43E-50	-1.69E-46
MS.gene049653	MS.gene95486	0.816581	5.14E-52	-1.69E-46
MS.gene049653	MS.gene99313	0.818564	1.84E-52	-1.69E-46
MS.gene049653	MS.gene99465	0.825562	4.38E-54	-1.69E-46
MS.gene049653	MS.gene99509	0.81686	4.45E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049653.t1	MTR_3g063970	98.592	71	1	0	1	71	1	71	2.86e-46	142
MS.gene049653.t1	MTR_3g063970	97.917	48	1	0	1	48	1	48	6.38e-16	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049653.t1	AT3G14430	65.079	63	21	1	9	71	15	76	1.03e-24	87.8

Find 15 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAACATCAATTCCTTTAAA+AGG	0.150864	3.2:-59546635	None:intergenic
ATTTCTTACGAAGGCGAAAA+TGG	0.322719	3.2:-59545631	None:intergenic
ACAAATACTTGATTGTATTC+AGG	0.336136	3.2:-59546580	None:intergenic
CTGGTGGTGGACCAGAGCTT+TGG	0.389186	3.2:+59546611	MS.gene049653:CDS
CTTTAAAAGGGCCAAAGCTC+TGG	0.402112	3.2:-59546622	None:intergenic
AAACATCAATTCCTTTAAAA+GGG	0.443719	3.2:-59546634	None:intergenic
CAAAGCTCTGGTCCACCACC+AGG	0.488950	3.2:-59546610	None:intergenic
CAAGTATTTGTGAAGAAGCC+TGG	0.508253	3.2:+59546592	MS.gene049653:CDS
TGAAGAAGAAAAGAAAGAAG+AGG	0.565354	3.2:-59545668	None:intergenic
AGAGAACAGCGATGGCTAGA+CGG	0.584999	3.2:+59545562	None:intergenic
CTAGACGGCGAGTCGAGACT+CGG	0.585126	3.2:+59545577	MS.gene049653:CDS
GGAACGAGTCGTTATTGACT+CGG	0.604165	3.2:+59545598	MS.gene049653:CDS
TTTGTGAAGAAGCCTGGTGG+TGG	0.604245	3.2:+59546598	MS.gene049653:CDS
AAACTCAGCATTTCTTACGA+AGG	0.638252	3.2:-59545640	None:intergenic
GTATTTGTGAAGAAGCCTGG+TGG	0.787083	3.2:+59546595	MS.gene049653:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGGATAATTATATCAAAA+AGG	+	chr3.2:59545904-59545923	MS.gene049653:intron	15.0%
!!!	TTTTGATAAGTGAATATTTT+TGG	+	chr3.2:59546437-59546456	MS.gene049653:intron	15.0%
!!	ACAAAAAAAAAAAAAGAGAG+AGG	-	chr3.2:59545731-59545750	None:intergenic	20.0%
!!	ATAATTCATTCATATATGGT+TGG	+	chr3.2:59546414-59546433	MS.gene049653:intron	20.0%
!!	CTTCATAATTCATTCATATA+TGG	+	chr3.2:59546410-59546429	MS.gene049653:intron	20.0%
!!	TTAGTTACTAAATTGAAAAC+TGG	+	chr3.2:59545870-59545889	MS.gene049653:intron	20.0%
!!	TTCACAAATTTATAGTATGT+CGG	+	chr3.2:59546143-59546162	MS.gene049653:intron	20.0%
!!!	CTTTTAATGTTTGAGAAATT+AGG	+	chr3.2:59545820-59545839	MS.gene049653:intron	20.0%
!	AACTATATACCAATTCCAAT+TGG	-	chr3.2:59545944-59545963	None:intergenic	25.0%
!	ACAAATACTTGATTGTATTC+AGG	-	chr3.2:59546583-59546602	None:intergenic	25.0%
!	ATTTGAACAAATGATGCAAA+TGG	-	chr3.2:59545767-59545786	None:intergenic	25.0%
!	TTAAATTACAATCAGCATGT+TGG	+	chr3.2:59546210-59546229	MS.gene049653:intron	25.0%
!	TTCAATGTAATCATATGTGT+CGG	+	chr3.2:59546335-59546354	MS.gene049653:intron	25.0%
!	TTTGAACAAATGATGCAAAT+GGG	-	chr3.2:59545766-59545785	None:intergenic	25.0%
!!	TTGTCTTGTAATTTCACTTT+TGG	+	chr3.2:59546512-59546531	MS.gene049653:intron	25.0%
!!!	AATTTTCGTTCTTTTTCATC+TGG	+	chr3.2:59545784-59545803	MS.gene049653:intron	25.0%
!!!	ATTTTCGTTCTTTTTCATCT+GGG	+	chr3.2:59545785-59545804	MS.gene049653:intron	25.0%
	ACTGATGAAAACAACTTACA+AGG	-	chr3.2:59546110-59546129	None:intergenic	30.0%
	ATTCTGTTTCCAATTGGAAT+TGG	+	chr3.2:59545932-59545951	MS.gene049653:intron	30.0%
	TGAAATTACAAGACAAACAG+AGG	-	chr3.2:59546508-59546527	None:intergenic	30.0%
	TGAAGAAGAAAAGAAAGAAG+AGG	-	chr3.2:59545671-59545690	None:intergenic	30.0%
	AAACTCAGCATTTCTTACGA+AGG	-	chr3.2:59545643-59545662	None:intergenic	35.0%
	AATTACAAGACAAACAGAGG+AGG	-	chr3.2:59546505-59546524	None:intergenic	35.0%
	ATTACAAGACAAACAGAGGA+GGG	-	chr3.2:59546504-59546523	None:intergenic	35.0%
	ATTTCTTACGAAGGCGAAAA+TGG	-	chr3.2:59545634-59545653	None:intergenic	35.0%
	GATTACGAGTCTGTTTGAAT+TGG	-	chr3.2:59546187-59546206	None:intergenic	35.0%
	GTAGAGATTCTGTTTCCAAT+TGG	+	chr3.2:59545926-59545945	MS.gene049653:intron	35.0%
!!	AAACTGGTTTGACGATTGAT+TGG	+	chr3.2:59545886-59545905	MS.gene049653:intron	35.0%
	CAAGTATTTGTGAAGAAGCC+TGG	+	chr3.2:59546592-59546611	MS.gene049653:CDS	40.0%
	TGGTGTATCATGTGTGAACA+CGG	+	chr3.2:59546237-59546256	MS.gene049653:intron	40.0%
	TTCTCAAATTAGGTGTGTCC+CGG	-	chr3.2:59546381-59546400	None:intergenic	40.0%
!!!	ACAATCAGCATGTTGGCTTT+TGG	+	chr3.2:59546217-59546236	MS.gene049653:intron	40.0%
	GCACGCACACTTCTCAAATT+AGG	-	chr3.2:59546391-59546410	None:intergenic	45.0%
	GTATTTGTGAAGAAGCCTGG+TGG	+	chr3.2:59546595-59546614	MS.gene049653:CDS	45.0%
	TGCAGACATCAGACACGAAA+CGG	+	chr3.2:59546267-59546286	MS.gene049653:intron	45.0%
!	GGAACGAGTCGTTATTGACT+CGG	+	chr3.2:59545598-59545617	MS.gene049653:CDS	45.0%
!!!	TTATTTTATCAAATTATTAT+TGG	-	chr3.2:59546310-59546329	None:intergenic	5.0%
	AAGACAAACAGAGGAGGGAG+CGG	-	chr3.2:59546499-59546518	None:intergenic	50.0%
	AATTAGGTGTGTCCCGGTGT+CGG	-	chr3.2:59546375-59546394	None:intergenic	50.0%
	ATCATGTGTGAACACGGACG+CGG	+	chr3.2:59546243-59546262	MS.gene049653:intron	50.0%
	CATCAGACACGAAACGGACA+TGG	+	chr3.2:59546273-59546292	MS.gene049653:intron	50.0%
	TTTGTGAAGAAGCCTGGTGG+TGG	+	chr3.2:59546598-59546617	MS.gene049653:CDS	50.0%
!!	CATATGTGTCGGTGTCGTGT+TGG	+	chr3.2:59546346-59546365	MS.gene049653:intron	50.0%
	AGACAAACAGAGGAGGGAGC+GGG	-	chr3.2:59546498-59546517	None:intergenic	55.0%
	ACAAACAGAGGAGGGAGCGG+GGG	-	chr3.2:59546496-59546515	None:intergenic	60.0%
	CTAGACGGCGAGTCGAGACT+CGG	+	chr3.2:59545577-59545596	MS.gene049653:CDS	60.0%
	GACAAACAGAGGAGGGAGCG+GGG	-	chr3.2:59546497-59546516	None:intergenic	60.0%
!	CAAAGCTCTGGTCCACCACC+AGG	-	chr3.2:59546613-59546632	None:intergenic	60.0%
!	GTCGTGTTGGTGTCCGACAC+CGG	+	chr3.2:59546359-59546378	MS.gene049653:intron	60.0%
!	TCGTGTTGGTGTCCGACACC+GGG	+	chr3.2:59546360-59546379	MS.gene049653:intron	60.0%
!!	CTGGTGGTGGACCAGAGCTT+TGG	+	chr3.2:59546611-59546630	MS.gene049653:CDS	60.0%
	AACAGAGGAGGGAGCGGGGG+TGG	-	chr3.2:59546493-59546512	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	59545573	59546642	59545573	ID=MS.gene049653
chr3.2	mRNA	59545573	59546642	59545573	ID=MS.gene049653.t1;Parent=MS.gene049653
chr3.2	exon	59545573	59545716	59545573	ID=MS.gene049653.t1.exon1;Parent=MS.gene049653.t1
chr3.2	CDS	59545573	59545716	59545573	ID=cds.MS.gene049653.t1;Parent=MS.gene049653.t1
chr3.2	exon	59546571	59546642	59546571	ID=MS.gene049653.t1.exon2;Parent=MS.gene049653.t1
chr3.2	CDS	59546571	59546642	59546571	ID=cds.MS.gene049653.t1;Parent=MS.gene049653.t1

Gene Sequence

>MS.gene049653

ATGGCTAGACGGCGAGTCGAGACTCGGAACGAGTCGTTATTGACTCGGATTGTTGATTCCATTTTCGCCTTCGTAAGAAATGCTGAGTTTGAGATCCTCTTCTTTCTTTTCTTCTTCATCGCTTATATTCTCTTCAAAGACATCGTAAGCTTCTTCCTCTCTCTTTTTTTTTTTTTGTGATTCATGAGCACCCATTTGCATCATTTGTTCAAATTTTCGTTCTTTTTCATCTGGGCATCTTGTTTCTCTTTTAATGTTTGAGAAATTAGGCTAAAATTTTACATTTTTCGTACTTGATTAGTTACTAAATTGAAAACTGGTTTGACGATTGATTGGATAATTATATCAAAAAGGTAGAGATTCTGTTTCCAATTGGAATTGGTATATAGTTTTGATAAGCAGCTTAATTAAAGCGCTTATAGTATAATTGCTTTTGTATAAGCTGTTTCTATAACATAATATGAAATAAAGTCAAACTATTTTCTTGTAAGCTATAAGTTGTTTTTGTAAGTTATCTAGAAGAGTATATAAACACCTTGTAAGTTGTTTTCATCAGTTCTTTCAATCAGATTCACAAATTTATAGTATGTCGGTAGCTAGACTTAAATAAGCCAATTCAAACAGACTCGTAATCATGTTAAATTACAATCAGCATGTTGGCTTTTGGTGTATCATGTGTGAACACGGACGCGGATGCAGACATCAGACACGAAACGGACATGGACACGCTGACACCAATAATAATTTGATAAAATAACATAATTCAATGTAATCATATGTGTCGGTGTCGTGTTGGTGTCCGACACCGGGACACACCTAATTTGAGAAGTGTGCGTGCTTCATAATTCATTCATATATGGTTGGTTTTGATAAGTGAATATTTTTGGCAACATGTCACATGTTCTGCATCACAACTTCCACCCCCGCTCCCTCCTCTGTTTGTCTTGTAATTTCACTTTTGGCAATGAGTTTCAATGACTGTTTTGCATAAAATGCAGACATCAAGACCTGAATACAATCAAGTATTTGTGAAGAAGCCTGGTGGTGGACCAGAGCTTTGGCCCTTTTAA

Protein sequence

>MS.gene049653.t1

MARRRVETRNESLLTRIVDSIFAFVRNAEFEILFFLFFFIAYILFKDITSRPEYNQVFVKKPGGGPELWPF