Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050161.t1 | XP_024640239.1 | 74.5 | 110 | 6 | 1 | 2 | 89 | 96 | 205 | 7.40E-36 | 159.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050161.t1 | G7JXF0 | 74.5 | 110 | 6 | 1 | 2 | 89 | 96 | 205 | 5.3e-36 | 159.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050148 | MS.gene050161 | 0.855459 | 6.22E-62 | -1.69E-46 |
| MS.gene050149 | MS.gene050161 | 0.834036 | 3.78E-56 | -1.69E-46 |
| MS.gene050161 | MS.gene09747 | 0.830024 | 3.71E-55 | -1.69E-46 |
| MS.gene050161 | MS.gene21214 | 0.834237 | 3.37E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050161.t1 | MTR_5g013030 | 74.545 | 110 | 6 | 1 | 2 | 89 | 96 | 205 | 3.53e-52 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTAGGCATAGGAACTTC+AGG | 0.345941 | 5.4:+6891916 | None:intergenic |
| GGTGAGGTTAAGGTTGAGAA+AGG | 0.514949 | 5.4:+6892071 | None:intergenic |
| ATAGTATTGGAACGGTGAAA+AGG | 0.522008 | 5.4:+6892050 | None:intergenic |
| AATCTGAAGAAAGCAAAGAA+TGG | 0.524107 | 5.4:-6891799 | MS.gene050161:CDS |
| AACTAGGCATAGGAACTTCA+GGG | 0.534743 | 5.4:+6891917 | None:intergenic |
| TCTCACACAGCACGCAATAC+AGG | 0.540563 | 5.4:-6891761 | None:intergenic |
| AGTCTTGGTCTTAGATAACA+TGG | 0.556996 | 5.4:-6891832 | MS.gene050161:intron |
| ACGGTGAAAAGGTGAGGTTA+AGG | 0.560123 | 5.4:+6892061 | None:intergenic |
| AGAACGTAATAGTATTGGAA+CGG | 0.607440 | 5.4:+6892042 | None:intergenic |
| ATTGGAACGGTGAAAAGGTG+AGG | 0.632983 | 5.4:+6892055 | None:intergenic |
| TAACTTGAATCACTTAAACA+TGG | 0.633744 | 5.4:+6891958 | None:intergenic |
| AAAGTCTTGTGGAGCAATTG+AGG | 0.638737 | 5.4:+6892004 | None:intergenic |
| GTCTTGGTCTTAGATAACAT+GGG | 0.707576 | 5.4:-6891831 | MS.gene050161:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAGAACGTAATAGTAT+TGG | + | chr5.4:6891800-6891819 | None:intergenic | 20.0% |
| ! | ATATAAGGGCTATATTTGAA+AGG | + | chr5.4:6891973-6891992 | None:intergenic | 25.0% |
| ! | ATATTTGAAAGGAATAGCAA+TGG | + | chr5.4:6891962-6891981 | None:intergenic | 25.0% |
| ! | TAACTTGAATCACTTAAACA+TGG | + | chr5.4:6891879-6891898 | None:intergenic | 25.0% |
| !!! | TTGACTTTTTTACATGAACT+AGG | + | chr5.4:6891936-6891955 | None:intergenic | 25.0% |
| AAGACTGTTGTCTGAATATA+AGG | + | chr5.4:6891988-6892007 | None:intergenic | 30.0% | |
| AATCTGAAGAAAGCAAAGAA+TGG | - | chr5.4:6892035-6892054 | MS.gene050161:CDS | 30.0% | |
| AGACTGTTGTCTGAATATAA+GGG | + | chr5.4:6891987-6892006 | None:intergenic | 30.0% | |
| TTATATTCAGACAACAGTCT+TGG | - | chr5.4:6891987-6892006 | MS.gene050161:CDS | 30.0% | |
| ! | AGAACGTAATAGTATTGGAA+CGG | + | chr5.4:6891795-6891814 | None:intergenic | 30.0% |
| ! | TTTTTACATGAACTAGGCAT+AGG | + | chr5.4:6891930-6891949 | None:intergenic | 30.0% |
| !! | TTTTGTTTGTGAAAGTCTTG+TGG | + | chr5.4:6891844-6891863 | None:intergenic | 30.0% |
| ! | ATAGTATTGGAACGGTGAAA+AGG | + | chr5.4:6891787-6891806 | None:intergenic | 35.0% |
| !! | AGTCTTGGTCTTAGATAACA+TGG | - | chr5.4:6892002-6892021 | MS.gene050161:CDS | 35.0% |
| !! | GTCTTGGTCTTAGATAACAT+GGG | - | chr5.4:6892003-6892022 | MS.gene050161:CDS | 35.0% |
| AACTAGGCATAGGAACTTCA+GGG | + | chr5.4:6891920-6891939 | None:intergenic | 40.0% | |
| ! | AAAGTCTTGTGGAGCAATTG+AGG | + | chr5.4:6891833-6891852 | None:intergenic | 40.0% |
| ACGGTGAAAAGGTGAGGTTA+AGG | + | chr5.4:6891776-6891795 | None:intergenic | 45.0% | |
| ATTGGAACGGTGAAAAGGTG+AGG | + | chr5.4:6891782-6891801 | None:intergenic | 45.0% | |
| GAACTAGGCATAGGAACTTC+AGG | + | chr5.4:6891921-6891940 | None:intergenic | 45.0% | |
| GGTGAGGTTAAGGTTGAGAA+AGG | + | chr5.4:6891766-6891785 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 6891762 | 6892094 | 6891762 | ID=MS.gene050161 |
| chr5.4 | mRNA | 6891762 | 6892094 | 6891762 | ID=MS.gene050161.t1;Parent=MS.gene050161 |
| chr5.4 | exon | 6891906 | 6892094 | 6891906 | ID=MS.gene050161.t1.exon1;Parent=MS.gene050161.t1 |
| chr5.4 | CDS | 6891906 | 6892094 | 6891906 | ID=cds.MS.gene050161.t1;Parent=MS.gene050161.t1 |
| chr5.4 | exon | 6891762 | 6891840 | 6891762 | ID=MS.gene050161.t1.exon2;Parent=MS.gene050161.t1 |
| chr5.4 | CDS | 6891762 | 6891840 | 6891762 | ID=cds.MS.gene050161.t1;Parent=MS.gene050161.t1 |
| Gene Sequence |
| Protein sequence |
