Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050148.t1 | XP_024640239.1 | 89.9 | 247 | 18 | 3 | 2 | 244 | 1 | 244 | 3.00E-119 | 438 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050148.t1 | Q9SKK8 | 31.6 | 177 | 106 | 7 | 32 | 199 | 43 | 213 | 9.3e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050148.t1 | G7JXF0 | 89.9 | 247 | 18 | 3 | 2 | 244 | 1 | 244 | 2.1e-119 | 438.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049409 | MS.gene050148 | 0.801949 | 7.07E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene050149 | 0.969114 | 1.19E-129 | -1.69E-46 |
| MS.gene050148 | MS.gene050161 | 0.855459 | 6.22E-62 | -1.69E-46 |
| MS.gene050148 | MS.gene050645 | 0.803842 | 2.87E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene050961 | 0.820051 | 8.41E-53 | -1.69E-46 |
| MS.gene050148 | MS.gene05480 | 0.80279 | 4.75E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene05861 | 0.839575 | 1.46E-57 | -1.69E-46 |
| MS.gene050148 | MS.gene05862 | 0.812836 | 3.47E-51 | -1.69E-46 |
| MS.gene050148 | MS.gene05969 | 0.840104 | 1.06E-57 | -1.69E-46 |
| MS.gene050148 | MS.gene063094 | 0.823372 | 1.44E-53 | -1.69E-46 |
| MS.gene050148 | MS.gene063690 | 0.81098 | 8.80E-51 | -1.69E-46 |
| MS.gene050148 | MS.gene06539 | 0.820866 | 5.47E-53 | -1.69E-46 |
| MS.gene050148 | MS.gene06784 | 0.820821 | 5.60E-53 | -1.69E-46 |
| MS.gene050148 | MS.gene09747 | 0.971488 | 3.05E-133 | -1.69E-46 |
| MS.gene050148 | MS.gene21214 | 0.986317 | 2.19E-166 | -1.69E-46 |
| MS.gene050148 | MS.gene25879 | 0.805869 | 1.08E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene32299 | 0.817786 | 2.75E-52 | -1.69E-46 |
| MS.gene050148 | MS.gene37404 | 0.812786 | 3.56E-51 | -1.69E-46 |
| MS.gene050148 | MS.gene38944 | 0.806896 | 6.58E-50 | -1.69E-46 |
| MS.gene050148 | MS.gene55126 | 0.816766 | 4.67E-52 | -1.69E-46 |
| MS.gene050148 | MS.gene56502 | 0.848911 | 4.53E-60 | -1.69E-46 |
| MS.gene050148 | MS.gene56725 | 0.813005 | 3.19E-51 | -1.69E-46 |
| MS.gene050148 | MS.gene57124 | 0.803871 | 2.83E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene63435 | 0.828446 | 8.95E-55 | -1.69E-46 |
| MS.gene050148 | MS.gene65297 | 0.806805 | 6.88E-50 | -1.69E-46 |
| MS.gene050148 | MS.gene65364 | 0.817804 | 2.73E-52 | -1.69E-46 |
| MS.gene050148 | MS.gene65365 | 0.803658 | 3.14E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene68141 | 0.804885 | 1.74E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene69852 | 0.813949 | 1.98E-51 | -1.69E-46 |
| MS.gene050148 | MS.gene70151 | 0.83934 | 1.68E-57 | -1.69E-46 |
| MS.gene050148 | MS.gene82340 | 0.835194 | 1.93E-56 | -1.69E-46 |
| MS.gene050148 | MS.gene83143 | 0.83886 | 2.24E-57 | -1.69E-46 |
| MS.gene050148 | MS.gene90939 | 0.807141 | 5.84E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050148.t1 | MTR_5g013030 | 91.903 | 247 | 13 | 3 | 2 | 244 | 1 | 244 | 9.41e-149 | 415 |
| MS.gene050148.t1 | MTR_3g091200 | 32.086 | 187 | 109 | 9 | 28 | 205 | 43 | 220 | 3.76e-15 | 74.3 |
| MS.gene050148.t1 | MTR_8g027745 | 31.098 | 164 | 98 | 6 | 30 | 185 | 33 | 189 | 8.70e-15 | 73.2 |
| MS.gene050148.t1 | MTR_4g073230 | 32.593 | 135 | 77 | 4 | 1 | 124 | 8 | 139 | 1.92e-12 | 66.6 |
| MS.gene050148.t1 | MTR_1g028290 | 26.667 | 225 | 148 | 7 | 22 | 235 | 37 | 255 | 4.93e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050148.t1 | AT5G53110 | 35.057 | 174 | 94 | 7 | 27 | 186 | 39 | 207 | 3.95e-17 | 80.1 |
| MS.gene050148.t1 | AT2G25410 | 28.807 | 243 | 136 | 10 | 31 | 242 | 42 | 278 | 4.06e-16 | 77.0 |
| MS.gene050148.t1 | AT2G46495 | 28.655 | 171 | 102 | 8 | 29 | 185 | 35 | 199 | 3.28e-13 | 68.6 |
| MS.gene050148.t1 | AT2G46495 | 28.655 | 171 | 102 | 8 | 29 | 185 | 35 | 199 | 3.62e-13 | 68.6 |
| MS.gene050148.t1 | AT5G36001 | 29.560 | 159 | 94 | 8 | 29 | 185 | 41 | 183 | 1.12e-11 | 62.8 |
| MS.gene050148.t1 | AT5G36001 | 29.560 | 159 | 94 | 8 | 29 | 185 | 41 | 183 | 3.90e-11 | 62.4 |
Find 42 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTTCTCACCCTCTTCT+TGG | 0.138462 | 5.4:-6609080 | MS.gene050148:CDS |
| TATGATGATACCACTCTTTC+TGG | 0.178660 | 5.4:-6608959 | MS.gene050148:CDS |
| TCACTATCCATTTCACATAA+AGG | 0.295402 | 5.4:-6609005 | MS.gene050148:CDS |
| AGACTGTTGTCTGAAATATA+AGG | 0.324770 | 5.4:+6608584 | None:intergenic |
| CACTATCCATTTCACATAAA+GGG | 0.339521 | 5.4:-6609004 | MS.gene050148:CDS |
| GAACTAGGCATAGGAACTTC+AGG | 0.345941 | 5.4:+6608650 | None:intergenic |
| CATAGCGTGGCAGATTTCTA+AGG | 0.365268 | 5.4:-6607898 | None:intergenic |
| AAGAAGAGTGAATTCATCAT+TGG | 0.384353 | 5.4:+6609097 | None:intergenic |
| GTTAACTGCAACGGTATTGC+TGG | 0.410536 | 5.4:-6609037 | MS.gene050148:CDS |
| TGTTGACCCTTTATGTGAAA+TGG | 0.413795 | 5.4:+6608998 | None:intergenic |
| CGTTGCAGTTAACAGAGGTT+TGG | 0.417106 | 5.4:+6609047 | None:intergenic |
| GGCAACACTTGTAGGCATAA+AGG | 0.435949 | 5.4:-6607956 | MS.gene050148:CDS |
| GACTGTTGTCTGAAATATAA+GGG | 0.447474 | 5.4:+6608585 | None:intergenic |
| TATATTTCAGACAACAGTCT+TGG | 0.476999 | 5.4:-6608581 | MS.gene050148:CDS |
| GCATAAAGGTTCATTTATCA+AGG | 0.478351 | 5.4:-6607942 | MS.gene050148:CDS |
| GTGGCACTGGCAACACTTGT+AGG | 0.486667 | 5.4:-6607964 | MS.gene050148:CDS |
| ACTATCAAATCACCATATGA+TGG | 0.503242 | 5.4:+6608893 | None:intergenic |
| TGAAATGGATAGTGAATGTC+AGG | 0.523321 | 5.4:+6609013 | None:intergenic |
| AATCTGAAGAAAGCAAAGAA+TGG | 0.524107 | 5.4:-6608532 | MS.gene050148:CDS |
| AACTAGGCATAGGAACTTCA+GGG | 0.534743 | 5.4:+6608651 | None:intergenic |
| CATTTATCAAGGAGGAAAGA+AGG | 0.537861 | 5.4:-6607931 | MS.gene050148:CDS |
| TCTCACACAGCACGCAATAC+AGG | 0.540563 | 5.4:-6608494 | MS.gene050148:intron |
| ACGGTGTAAAGGTGAGGTTA+AGG | 0.543625 | 5.4:+6608795 | None:intergenic |
| ATAGTATTGGAACGGTGTAA+AGG | 0.555489 | 5.4:+6608784 | None:intergenic |
| ACCATATGATGGAAAATGAA+TGG | 0.555827 | 5.4:+6608904 | None:intergenic |
| ATATAAGGGCTATATTTGAA+AGG | 0.590983 | 5.4:+6608599 | None:intergenic |
| GAAGATGAAAACCAAGAAGA+GGG | 0.591458 | 5.4:+6609069 | None:intergenic |
| AGGACAATTGTTGCATAGCG+TGG | 0.602254 | 5.4:-6607911 | MS.gene050148:CDS |
| AAGCTCAAAACCAGAAAGAG+TGG | 0.602365 | 5.4:+6608949 | None:intergenic |
| AGAACGTAATAGTATTGGAA+CGG | 0.607440 | 5.4:+6608776 | None:intergenic |
| GGAAGATGAAAACCAAGAAG+AGG | 0.611027 | 5.4:+6609068 | None:intergenic |
| CAAACCTCTGTTAACTGCAA+CGG | 0.615436 | 5.4:-6609046 | MS.gene050148:CDS |
| ATATTTGAAAGGAATAGCAA+TGG | 0.622554 | 5.4:+6608610 | None:intergenic |
| TAACTTGAATCACTTAAACA+TGG | 0.633744 | 5.4:+6608692 | None:intergenic |
| GTCTTGGTCTTAGATTAACA+TGG | 0.638466 | 5.4:-6608565 | MS.gene050148:CDS |
| AAAGTCTTGTGGAGCAATTG+AGG | 0.638737 | 5.4:+6608738 | None:intergenic |
| TAAAGGTTCATTTATCAAGG+AGG | 0.641975 | 5.4:-6607939 | MS.gene050148:CDS |
| ATTGGAACGGTGTAAAGGTG+AGG | 0.642041 | 5.4:+6608789 | None:intergenic |
| TCTTGGTCTTAGATTAACAT+GGG | 0.645510 | 5.4:-6608564 | MS.gene050148:CDS |
| AATACCGTTGCAGTTAACAG+AGG | 0.663218 | 5.4:+6609042 | None:intergenic |
| TAAGGTTGAGAAAGACTCTG+TGG | 0.692883 | 5.4:+6608813 | None:intergenic |
| ATATGATGGAAAATGAATGG+TGG | 0.729816 | 5.4:+6608907 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTACAAAAATAAAGTTCA+TGG | - | chr5.4:6608548-6608567 | MS.gene050148:CDS | 15.0% |
| !! | ATTACAAAAATAAAGTTCAT+GGG | - | chr5.4:6608549-6608568 | MS.gene050148:CDS | 15.0% |
| !! | GTATAGTACATATATATATT+GGG | - | chr5.4:6608685-6608704 | MS.gene050148:CDS | 15.0% |
| !! | TGTATAGTACATATATATAT+TGG | - | chr5.4:6608684-6608703 | MS.gene050148:CDS | 15.0% |
| !! | AGAAAATTATTAACAAAGCT+TGG | - | chr5.4:6608912-6608931 | MS.gene050148:CDS | 20.0% |
| !! | TTTGTAATTAAAGAAAGAGA+TGG | + | chr5.4:6608537-6608556 | None:intergenic | 20.0% |
| !!! | TTTTAAGAACGTAATAGTAT+TGG | + | chr5.4:6608226-6608245 | None:intergenic | 20.0% |
| ! | AGGAAATTATGCTCATTTAA+AGG | + | chr5.4:6608593-6608612 | None:intergenic | 25.0% |
| ! | AGTACATATATATATTGGGA+AGG | - | chr5.4:6608689-6608708 | MS.gene050148:CDS | 25.0% |
| ! | ATAATTTCCTTATACTAGGT+TGG | - | chr5.4:6608603-6608622 | MS.gene050148:CDS | 25.0% |
| ! | ATATAAGGGCTATATTTGAA+AGG | + | chr5.4:6608398-6608417 | None:intergenic | 25.0% |
| ! | ATATTTGAAAGGAATAGCAA+TGG | + | chr5.4:6608387-6608406 | None:intergenic | 25.0% |
| ! | ATCATTACGAAGTATATACT+AGG | - | chr5.4:6608743-6608762 | MS.gene050148:CDS | 25.0% |
| ! | TAACTTGAATCACTTAAACA+TGG | + | chr5.4:6608305-6608324 | None:intergenic | 25.0% |
| ! | TAATTTCCTTATACTAGGTT+GGG | - | chr5.4:6608604-6608623 | MS.gene050148:CDS | 25.0% |
| !! | ATCAAGAAGATCAATCTTTT+TGG | + | chr5.4:6608147-6608166 | None:intergenic | 25.0% |
| !!! | TTGACTTTTTTACATGAACT+AGG | + | chr5.4:6608362-6608381 | None:intergenic | 25.0% |
| AATCTGAAGAAAGCAAAGAA+TGG | - | chr5.4:6608462-6608481 | MS.gene050148:intron | 30.0% | |
| ACTATCAAATCACCATATGA+TGG | + | chr5.4:6608104-6608123 | None:intergenic | 30.0% | |
| CACTATCCATTTCACATAAA+GGG | - | chr5.4:6607990-6608009 | MS.gene050148:CDS | 30.0% | |
| GAGCATAATTTCCTTATACT+AGG | - | chr5.4:6608599-6608618 | MS.gene050148:CDS | 30.0% | |
| GCATAAAGGTTCATTTATCA+AGG | - | chr5.4:6609052-6609071 | MS.gene050148:CDS | 30.0% | |
| TAAAGGTTCATTTATCAAGG+AGG | - | chr5.4:6609055-6609074 | MS.gene050148:CDS | 30.0% | |
| TACGAAGTATATACTAGGTT+GGG | - | chr5.4:6608748-6608767 | MS.gene050148:CDS | 30.0% | |
| TAGTACATTATCTTTCGTGT+AGG | - | chr5.4:6608810-6608829 | MS.gene050148:CDS | 30.0% | |
| TATATTTCAGACAACAGTCT+TGG | - | chr5.4:6608413-6608432 | MS.gene050148:intron | 30.0% | |
| TCACTATCCATTTCACATAA+AGG | - | chr5.4:6607989-6608008 | MS.gene050148:CDS | 30.0% | |
| TTACGAAGTATATACTAGGT+TGG | - | chr5.4:6608747-6608766 | MS.gene050148:CDS | 30.0% | |
| ! | ACCATATGATGGAAAATGAA+TGG | + | chr5.4:6608093-6608112 | None:intergenic | 30.0% |
| ! | ACCATTCATTTTCCATCATA+TGG | - | chr5.4:6608089-6608108 | MS.gene050148:intron | 30.0% |
| ! | AGAACGTAATAGTATTGGAA+CGG | + | chr5.4:6608221-6608240 | None:intergenic | 30.0% |
| ! | AGACTGTTGTCTGAAATATA+AGG | + | chr5.4:6608413-6608432 | None:intergenic | 30.0% |
| ! | ATATGATGGAAAATGAATGG+TGG | + | chr5.4:6608090-6608109 | None:intergenic | 30.0% |
| ! | GACTGTTGTCTGAAATATAA+GGG | + | chr5.4:6608412-6608431 | None:intergenic | 30.0% |
| ! | TTTTTACATGAACTAGGCAT+AGG | + | chr5.4:6608356-6608375 | None:intergenic | 30.0% |
| !! | TCTTGGTCTTAGATTAACAT+GGG | - | chr5.4:6608430-6608449 | MS.gene050148:intron | 30.0% |
| !! | TTTTGTTTGTGAAAGTCTTG+TGG | + | chr5.4:6608270-6608289 | None:intergenic | 30.0% |
| !!! | ATCAATCTTTTTGGTGTCAT+AGG | + | chr5.4:6608138-6608157 | None:intergenic | 30.0% |
| AAACTACCCAACCTAGTATA+AGG | + | chr5.4:6608613-6608632 | None:intergenic | 35.0% | |
| CATTTATCAAGGAGGAAAGA+AGG | - | chr5.4:6609063-6609082 | MS.gene050148:CDS | 35.0% | |
| GAAGATGAAAACCAAGAAGA+GGG | + | chr5.4:6607928-6607947 | None:intergenic | 35.0% | |
| TATGATGATACCACTCTTTC+TGG | - | chr5.4:6608035-6608054 | MS.gene050148:intron | 35.0% | |
| TCTTTCGTGTAGGATTGATT+CGG | - | chr5.4:6608820-6608839 | MS.gene050148:CDS | 35.0% | |
| TGAAATGGATAGTGAATGTC+AGG | + | chr5.4:6607984-6608003 | None:intergenic | 35.0% | |
| TGTTGACCCTTTATGTGAAA+TGG | + | chr5.4:6607999-6608018 | None:intergenic | 35.0% | |
| ! | ATAGTATTGGAACGGTGTAA+AGG | + | chr5.4:6608213-6608232 | None:intergenic | 35.0% |
| ! | ATATACTAGGTTGGGTAGTT+TGG | - | chr5.4:6608756-6608775 | MS.gene050148:CDS | 35.0% |
| !! | GTCTTGGTCTTAGATTAACA+TGG | - | chr5.4:6608429-6608448 | MS.gene050148:intron | 35.0% |
| !!! | CTTTTTGGTGTCATAGGAAA+TGG | + | chr5.4:6608132-6608151 | None:intergenic | 35.0% |
| AACTAGGCATAGGAACTTCA+GGG | + | chr5.4:6608346-6608365 | None:intergenic | 40.0% | |
| AAGCTCAAAACCAGAAAGAG+TGG | + | chr5.4:6608048-6608067 | None:intergenic | 40.0% | |
| AATACCGTTGCAGTTAACAG+AGG | + | chr5.4:6607955-6607974 | None:intergenic | 40.0% | |
| CAAACCTCTGTTAACTGCAA+CGG | - | chr5.4:6607948-6607967 | MS.gene050148:CDS | 40.0% | |
| GGAAGATGAAAACCAAGAAG+AGG | + | chr5.4:6607929-6607948 | None:intergenic | 40.0% | |
| TAAGGTTGAGAAAGACTCTG+TGG | + | chr5.4:6608184-6608203 | None:intergenic | 40.0% | |
| TTCAACACATGTGCAGTTCT+TGG | - | chr5.4:6608985-6609004 | MS.gene050148:CDS | 40.0% | |
| ! | AAAGTCTTGTGGAGCAATTG+AGG | + | chr5.4:6608259-6608278 | None:intergenic | 40.0% |
| ! | TCTCAGCATCTGTATTTTCG+TGG | - | chr5.4:6609008-6609027 | MS.gene050148:CDS | 40.0% |
| !!! | ACTCTGTGGACACAGTTTTT+TGG | + | chr5.4:6608170-6608189 | None:intergenic | 40.0% |
| ACGGTGTAAAGGTGAGGTTA+AGG | + | chr5.4:6608202-6608221 | None:intergenic | 45.0% | |
| AGGACAATTGTTGCATAGCG+TGG | - | chr5.4:6609083-6609102 | MS.gene050148:CDS | 45.0% | |
| ATTGGAACGGTGTAAAGGTG+AGG | + | chr5.4:6608208-6608227 | None:intergenic | 45.0% | |
| CGTTGCAGTTAACAGAGGTT+TGG | + | chr5.4:6607950-6607969 | None:intergenic | 45.0% | |
| CTTCTTTCTCACCCTCTTCT+TGG | - | chr5.4:6607914-6607933 | MS.gene050148:CDS | 45.0% | |
| GAACTAGGCATAGGAACTTC+AGG | + | chr5.4:6608347-6608366 | None:intergenic | 45.0% | |
| GGCAACACTTGTAGGCATAA+AGG | - | chr5.4:6609038-6609057 | MS.gene050148:CDS | 45.0% | |
| GTTAACTGCAACGGTATTGC+TGG | - | chr5.4:6607957-6607976 | MS.gene050148:CDS | 45.0% | |
| ! | CAGCATCTGTATTTTCGTGG+TGG | - | chr5.4:6609011-6609030 | MS.gene050148:CDS | 45.0% |
| TCTCACACAGCACGCAATAC+AGG | - | chr5.4:6608500-6608519 | MS.gene050148:CDS | 50.0% | |
| ! | CTGTATTTTCGTGGTGGCAC+TGG | - | chr5.4:6609017-6609036 | MS.gene050148:CDS | 50.0% |
| !! | GTGGCACTGGCAACACTTGT+AGG | - | chr5.4:6609030-6609049 | MS.gene050148:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 6607900 | 6609116 | 6607900 | ID=MS.gene050148 |
| chr5.4 | mRNA | 6607900 | 6609116 | 6607900 | ID=MS.gene050148.t1;Parent=MS.gene050148 |
| chr5.4 | exon | 6608495 | 6609116 | 6608495 | ID=MS.gene050148.t1.exon1;Parent=MS.gene050148.t1 |
| chr5.4 | CDS | 6608495 | 6609116 | 6608495 | ID=cds.MS.gene050148.t1;Parent=MS.gene050148.t1 |
| chr5.4 | exon | 6607900 | 6608015 | 6607900 | ID=MS.gene050148.t1.exon2;Parent=MS.gene050148.t1 |
| chr5.4 | CDS | 6607900 | 6608015 | 6607900 | ID=cds.MS.gene050148.t1;Parent=MS.gene050148.t1 |
| Gene Sequence |
| Protein sequence |
