Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050149.t1 | XP_024640239.1 | 86.5 | 207 | 25 | 2 | 2 | 206 | 1 | 206 | 3.00E-96 | 361.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050149.t1 | Q9SKK8 | 33.1 | 175 | 99 | 8 | 32 | 194 | 43 | 211 | 4.6e-13 | 76.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050149.t1 | G7JXF0 | 86.5 | 207 | 25 | 2 | 2 | 206 | 1 | 206 | 2.2e-96 | 361.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050148 | MS.gene050149 | 0.969114 | 1.19E-129 | -1.69E-46 |
| MS.gene050149 | MS.gene050161 | 0.834036 | 3.78E-56 | -1.69E-46 |
| MS.gene050149 | MS.gene050961 | 0.815644 | 8.32E-52 | -1.69E-46 |
| MS.gene050149 | MS.gene05861 | 0.817831 | 2.69E-52 | -1.69E-46 |
| MS.gene050149 | MS.gene05862 | 0.800796 | 1.22E-48 | -1.69E-46 |
| MS.gene050149 | MS.gene05969 | 0.819625 | 1.05E-52 | -1.69E-46 |
| MS.gene050149 | MS.gene063690 | 0.803845 | 2.87E-49 | -1.69E-46 |
| MS.gene050149 | MS.gene067567 | 0.803327 | 3.68E-49 | -1.69E-46 |
| MS.gene050149 | MS.gene072560 | 0.812034 | 5.19E-51 | -1.69E-46 |
| MS.gene050149 | MS.gene09747 | 0.974423 | 3.95E-138 | -1.69E-46 |
| MS.gene050149 | MS.gene21214 | 0.962462 | 6.61E-121 | -1.69E-46 |
| MS.gene050149 | MS.gene55126 | 0.802758 | 4.82E-49 | -1.69E-46 |
| MS.gene050149 | MS.gene56502 | 0.828184 | 1.04E-54 | -1.69E-46 |
| MS.gene050149 | MS.gene65297 | 0.804255 | 2.36E-49 | -1.69E-46 |
| MS.gene050149 | MS.gene69852 | 0.80678 | 6.97E-50 | -1.69E-46 |
| MS.gene050149 | MS.gene82340 | 0.801405 | 9.15E-49 | -1.69E-46 |
| MS.gene050149 | MS.gene83143 | 0.804287 | 2.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050149.t1 | MTR_5g013030 | 88.889 | 207 | 20 | 2 | 2 | 206 | 1 | 206 | 1.58e-117 | 334 |
| MS.gene050149.t1 | MTR_8g027745 | 30.488 | 164 | 99 | 5 | 30 | 185 | 33 | 189 | 5.54e-15 | 72.8 |
| MS.gene050149.t1 | MTR_3g091200 | 32.044 | 181 | 106 | 8 | 28 | 199 | 43 | 215 | 6.50e-15 | 72.8 |
| MS.gene050149.t1 | MTR_4g073230 | 32.593 | 135 | 77 | 4 | 1 | 124 | 8 | 139 | 1.52e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050149.t1 | AT5G53110 | 32.338 | 201 | 108 | 8 | 27 | 204 | 39 | 234 | 6.59e-18 | 81.3 |
| MS.gene050149.t1 | AT2G25410 | 32.955 | 176 | 100 | 8 | 31 | 194 | 42 | 211 | 2.13e-16 | 77.0 |
| MS.gene050149.t1 | AT2G46495 | 28.655 | 171 | 102 | 8 | 29 | 185 | 35 | 199 | 1.52e-13 | 68.6 |
| MS.gene050149.t1 | AT2G46495 | 28.655 | 171 | 102 | 8 | 29 | 185 | 35 | 199 | 1.57e-13 | 68.6 |
| MS.gene050149.t1 | AT5G36001 | 29.560 | 159 | 94 | 8 | 29 | 185 | 41 | 183 | 4.68e-12 | 63.2 |
| MS.gene050149.t1 | AT5G36001 | 29.560 | 159 | 94 | 8 | 29 | 185 | 41 | 183 | 2.10e-11 | 62.4 |
Find 35 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTTCTCACCCTCTTCT+TGG | 0.138462 | 5.4:+6617902 | MS.gene050149:CDS |
| TATGATGATACCACTCTTTC+TGG | 0.178660 | 5.4:+6618023 | MS.gene050149:CDS |
| TCACTATCCATTTCACATAA+AGG | 0.295402 | 5.4:+6617977 | MS.gene050149:CDS |
| AGACTGTTGTCTGAAATATA+AGG | 0.324770 | 5.4:-6618398 | None:intergenic |
| CACTATCCATTTCACATAAA+GGG | 0.339521 | 5.4:+6617978 | MS.gene050149:CDS |
| GAACTAGGCATAGGAACTTC+AGG | 0.345941 | 5.4:-6618332 | None:intergenic |
| AAGAAGAGTGAATTCATCAT+TGG | 0.384353 | 5.4:-6617885 | None:intergenic |
| GTTAACTGCAACGGTATTGC+TGG | 0.410536 | 5.4:+6617945 | MS.gene050149:CDS |
| TGTTGACCCTTTATGTGAAA+TGG | 0.413795 | 5.4:-6617984 | None:intergenic |
| CGTTGCAGTTAACAGAGGTT+TGG | 0.417106 | 5.4:-6617935 | None:intergenic |
| GACTGTTGTCTGAAATATAA+GGG | 0.447474 | 5.4:-6618397 | None:intergenic |
| TATATTTCAGACAACAGTCT+TGG | 0.476999 | 5.4:+6618401 | MS.gene050149:CDS |
| ACTATCAAATCACCATATGA+TGG | 0.503242 | 5.4:-6618089 | None:intergenic |
| TGAAATGGATAGTGAATGTC+AGG | 0.523321 | 5.4:-6617969 | None:intergenic |
| AACTAGGCATAGGAACTTCA+GGG | 0.534743 | 5.4:-6618331 | None:intergenic |
| TCTCACACAGCACGCAATAC+AGG | 0.540563 | 5.4:+6618487 | MS.gene050149:CDS |
| ACGGTGTAAAGGTGAGGTTA+AGG | 0.543625 | 5.4:-6618187 | None:intergenic |
| GAATCTGAGAAAGCAAAGAA+TGG | 0.549126 | 5.4:+6618449 | MS.gene050149:CDS |
| ATAGTATTGGAACGGTGTAA+AGG | 0.555489 | 5.4:-6618198 | None:intergenic |
| ACCATATGATGGAAAATGAA+TGG | 0.555827 | 5.4:-6618078 | None:intergenic |
| ATATAAGGGCTATATTTGAA+AGG | 0.590983 | 5.4:-6618383 | None:intergenic |
| GAAGATGAAAACCAAGAAGA+GGG | 0.591458 | 5.4:-6617913 | None:intergenic |
| AAGCTCAAAACCAGAAAGAG+TGG | 0.602365 | 5.4:-6618033 | None:intergenic |
| AGAACGTAATAGTATTGGAA+CGG | 0.607440 | 5.4:-6618206 | None:intergenic |
| GGAAGATGAAAACCAAGAAG+AGG | 0.611027 | 5.4:-6617914 | None:intergenic |
| CAAACCTCTGTTAACTGCAA+CGG | 0.615436 | 5.4:+6617936 | MS.gene050149:CDS |
| ATATTTGAAAGGAATAGCAA+TGG | 0.622554 | 5.4:-6618372 | None:intergenic |
| TAACTTGAATCACTTAAACA+TGG | 0.633744 | 5.4:-6618290 | None:intergenic |
| GTCTTGGTCTTAGATTAACA+TGG | 0.638466 | 5.4:+6618417 | MS.gene050149:CDS |
| AAAGTCTTGTGGAGCAATTG+AGG | 0.638737 | 5.4:-6618244 | None:intergenic |
| ATTGGAACGGTGTAAAGGTG+AGG | 0.642041 | 5.4:-6618193 | None:intergenic |
| TCTTGGTCTTAGATTAACAT+GGG | 0.645510 | 5.4:+6618418 | MS.gene050149:CDS |
| AATACCGTTGCAGTTAACAG+AGG | 0.663218 | 5.4:-6617940 | None:intergenic |
| TAAGGTTGAGAAAGACTCTG+TGG | 0.692883 | 5.4:-6618169 | None:intergenic |
| ATATGATGGAAAATGAATGG+TGG | 0.729816 | 5.4:-6618075 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAGAACGTAATAGTAT+TGG | - | chr5.4:6618214-6618233 | None:intergenic | 20.0% |
| ! | ATATAAGGGCTATATTTGAA+AGG | - | chr5.4:6618386-6618405 | None:intergenic | 25.0% |
| ! | ATATTTGAAAGGAATAGCAA+TGG | - | chr5.4:6618375-6618394 | None:intergenic | 25.0% |
| ! | TAACTTGAATCACTTAAACA+TGG | - | chr5.4:6618293-6618312 | None:intergenic | 25.0% |
| !! | ATCAAGAAGATCAATCTTTT+TGG | - | chr5.4:6618135-6618154 | None:intergenic | 25.0% |
| !!! | TTGACTTTTTTACATGAACT+AGG | - | chr5.4:6618350-6618369 | None:intergenic | 25.0% |
| ACTATCAAATCACCATATGA+TGG | - | chr5.4:6618092-6618111 | None:intergenic | 30.0% | |
| CACTATCCATTTCACATAAA+GGG | + | chr5.4:6617978-6617997 | MS.gene050149:CDS | 30.0% | |
| TATATTTCAGACAACAGTCT+TGG | + | chr5.4:6618401-6618420 | MS.gene050149:CDS | 30.0% | |
| TCACTATCCATTTCACATAA+AGG | + | chr5.4:6617977-6617996 | MS.gene050149:CDS | 30.0% | |
| ! | ACCATATGATGGAAAATGAA+TGG | - | chr5.4:6618081-6618100 | None:intergenic | 30.0% |
| ! | ACCATTCATTTTCCATCATA+TGG | + | chr5.4:6618077-6618096 | MS.gene050149:CDS | 30.0% |
| ! | AGAACGTAATAGTATTGGAA+CGG | - | chr5.4:6618209-6618228 | None:intergenic | 30.0% |
| ! | AGACTGTTGTCTGAAATATA+AGG | - | chr5.4:6618401-6618420 | None:intergenic | 30.0% |
| ! | ATATGATGGAAAATGAATGG+TGG | - | chr5.4:6618078-6618097 | None:intergenic | 30.0% |
| ! | GACTGTTGTCTGAAATATAA+GGG | - | chr5.4:6618400-6618419 | None:intergenic | 30.0% |
| ! | TTTTTACATGAACTAGGCAT+AGG | - | chr5.4:6618344-6618363 | None:intergenic | 30.0% |
| !! | TCTTGGTCTTAGATTAACAT+GGG | + | chr5.4:6618418-6618437 | MS.gene050149:CDS | 30.0% |
| !! | TTTTGTTTGTGAAAGTCTTG+TGG | - | chr5.4:6618258-6618277 | None:intergenic | 30.0% |
| !!! | ATCAATCTTTTTGGTGTCAT+AGG | - | chr5.4:6618126-6618145 | None:intergenic | 30.0% |
| GAAGATGAAAACCAAGAAGA+GGG | - | chr5.4:6617916-6617935 | None:intergenic | 35.0% | |
| GAATCTGAGAAAGCAAAGAA+TGG | + | chr5.4:6618449-6618468 | MS.gene050149:CDS | 35.0% | |
| TATGATGATACCACTCTTTC+TGG | + | chr5.4:6618023-6618042 | MS.gene050149:CDS | 35.0% | |
| TGAAATGGATAGTGAATGTC+AGG | - | chr5.4:6617972-6617991 | None:intergenic | 35.0% | |
| TGTTGACCCTTTATGTGAAA+TGG | - | chr5.4:6617987-6618006 | None:intergenic | 35.0% | |
| ! | ATAGTATTGGAACGGTGTAA+AGG | - | chr5.4:6618201-6618220 | None:intergenic | 35.0% |
| !! | GTCTTGGTCTTAGATTAACA+TGG | + | chr5.4:6618417-6618436 | MS.gene050149:CDS | 35.0% |
| !!! | CTTTTTGGTGTCATAGGAAA+TGG | - | chr5.4:6618120-6618139 | None:intergenic | 35.0% |
| AACTAGGCATAGGAACTTCA+GGG | - | chr5.4:6618334-6618353 | None:intergenic | 40.0% | |
| AAGCTCAAAACCAGAAAGAG+TGG | - | chr5.4:6618036-6618055 | None:intergenic | 40.0% | |
| AATACCGTTGCAGTTAACAG+AGG | - | chr5.4:6617943-6617962 | None:intergenic | 40.0% | |
| CAAACCTCTGTTAACTGCAA+CGG | + | chr5.4:6617936-6617955 | MS.gene050149:CDS | 40.0% | |
| GGAAGATGAAAACCAAGAAG+AGG | - | chr5.4:6617917-6617936 | None:intergenic | 40.0% | |
| TAAGGTTGAGAAAGACTCTG+TGG | - | chr5.4:6618172-6618191 | None:intergenic | 40.0% | |
| ! | AAAGTCTTGTGGAGCAATTG+AGG | - | chr5.4:6618247-6618266 | None:intergenic | 40.0% |
| !!! | ACTCTGTGGACACAGTTTTT+TGG | - | chr5.4:6618158-6618177 | None:intergenic | 40.0% |
| ACGGTGTAAAGGTGAGGTTA+AGG | - | chr5.4:6618190-6618209 | None:intergenic | 45.0% | |
| ATTGGAACGGTGTAAAGGTG+AGG | - | chr5.4:6618196-6618215 | None:intergenic | 45.0% | |
| CGTTGCAGTTAACAGAGGTT+TGG | - | chr5.4:6617938-6617957 | None:intergenic | 45.0% | |
| CTTCTTTCTCACCCTCTTCT+TGG | + | chr5.4:6617902-6617921 | MS.gene050149:CDS | 45.0% | |
| GAACTAGGCATAGGAACTTC+AGG | - | chr5.4:6618335-6618354 | None:intergenic | 45.0% | |
| GTTAACTGCAACGGTATTGC+TGG | + | chr5.4:6617945-6617964 | MS.gene050149:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 6617888 | 6618508 | 6617888 | ID=MS.gene050149 |
| chr5.4 | mRNA | 6617888 | 6618508 | 6617888 | ID=MS.gene050149.t1;Parent=MS.gene050149 |
| chr5.4 | exon | 6617888 | 6618508 | 6617888 | ID=MS.gene050149.t1.exon1;Parent=MS.gene050149.t1 |
| chr5.4 | CDS | 6617888 | 6618508 | 6617888 | ID=cds.MS.gene050149.t1;Parent=MS.gene050149.t1 |
| Gene Sequence |
| Protein sequence |
