Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21214.t1 | XP_024640239.1 | 89.9 | 247 | 18 | 3 | 1 | 243 | 1 | 244 | 2.30E-119 | 438.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21214.t1 | Q9SKK8 | 31.6 | 177 | 106 | 7 | 31 | 198 | 43 | 213 | 9.3e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21214.t1 | G7JXF0 | 89.9 | 247 | 18 | 3 | 1 | 243 | 1 | 244 | 1.6e-119 | 438.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049409 | MS.gene21214 | 0.805358 | 1.39E-49 | -1.69E-46 |
| MS.gene050148 | MS.gene21214 | 0.986317 | 2.19E-166 | -1.69E-46 |
| MS.gene050149 | MS.gene21214 | 0.962462 | 6.61E-121 | -1.69E-46 |
| MS.gene050161 | MS.gene21214 | 0.834237 | 3.37E-56 | -1.69E-46 |
| MS.gene050645 | MS.gene21214 | 0.805212 | 1.49E-49 | -1.69E-46 |
| MS.gene050961 | MS.gene21214 | 0.823514 | 1.33E-53 | -1.69E-46 |
| MS.gene05480 | MS.gene21214 | 0.802565 | 5.28E-49 | -1.69E-46 |
| MS.gene05861 | MS.gene21214 | 0.836182 | 1.09E-56 | -1.69E-46 |
| MS.gene05862 | MS.gene21214 | 0.818581 | 1.82E-52 | -1.69E-46 |
| MS.gene05969 | MS.gene21214 | 0.836625 | 8.38E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21214.t1 | MTR_5g013030 | 91.903 | 247 | 13 | 3 | 1 | 243 | 1 | 244 | 1.75e-148 | 414 |
| MS.gene21214.t1 | MTR_3g091200 | 32.086 | 187 | 109 | 9 | 27 | 204 | 43 | 220 | 3.58e-15 | 74.3 |
| MS.gene21214.t1 | MTR_8g027745 | 29.878 | 164 | 100 | 5 | 29 | 184 | 33 | 189 | 8.45e-15 | 73.2 |
| MS.gene21214.t1 | MTR_4g073230 | 35.514 | 107 | 63 | 3 | 20 | 123 | 36 | 139 | 2.71e-12 | 66.2 |
| MS.gene21214.t1 | MTR_1g028290 | 26.667 | 225 | 148 | 7 | 21 | 234 | 37 | 255 | 5.42e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21214.t1 | AT5G53110 | 35.057 | 174 | 94 | 7 | 26 | 185 | 39 | 207 | 3.62e-17 | 80.1 |
| MS.gene21214.t1 | AT2G25410 | 28.807 | 243 | 136 | 10 | 30 | 241 | 42 | 278 | 4.13e-16 | 77.0 |
| MS.gene21214.t1 | AT2G46495 | 28.655 | 171 | 102 | 8 | 28 | 184 | 35 | 199 | 2.97e-13 | 68.9 |
| MS.gene21214.t1 | AT2G46495 | 28.655 | 171 | 102 | 8 | 28 | 184 | 35 | 199 | 3.32e-13 | 68.6 |
| MS.gene21214.t1 | AT5G36001 | 29.560 | 159 | 94 | 8 | 28 | 184 | 41 | 183 | 1.26e-11 | 62.8 |
| MS.gene21214.t1 | AT5G36001 | 29.560 | 159 | 94 | 8 | 28 | 184 | 41 | 183 | 4.03e-11 | 62.4 |
Find 41 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTTCTCACCCTCTTCT+TGG | 0.138462 | 5.1:-6109499 | MS.gene21214:CDS |
| TATGATGATACCACTCTTTC+TGG | 0.178660 | 5.1:-6109378 | MS.gene21214:CDS |
| TCACTATCCATTTCACATAA+AGG | 0.295402 | 5.1:-6109424 | MS.gene21214:CDS |
| AGACTGTTGTCTGAAATATA+AGG | 0.324770 | 5.1:+6109003 | None:intergenic |
| CACTATCCATTTCACATAAA+GGG | 0.339521 | 5.1:-6109423 | MS.gene21214:CDS |
| GAACTAGGCATAGGAACTTC+AGG | 0.345941 | 5.1:+6109069 | None:intergenic |
| CATAGCGTGGCAGATTTCTA+AGG | 0.365268 | 5.1:-6108317 | None:intergenic |
| GTTAACTGCAACGGTATTGC+TGG | 0.410536 | 5.1:-6109456 | MS.gene21214:CDS |
| TGTTGACCCTTTATGTGAAA+TGG | 0.413795 | 5.1:+6109417 | None:intergenic |
| CGTTGCAGTTAACAGAGGTT+TGG | 0.417106 | 5.1:+6109466 | None:intergenic |
| GGCAACACTTGTAGGCATAA+AGG | 0.435949 | 5.1:-6108375 | MS.gene21214:CDS |
| GACTGTTGTCTGAAATATAA+GGG | 0.447474 | 5.1:+6109004 | None:intergenic |
| TATATTTCAGACAACAGTCT+TGG | 0.476999 | 5.1:-6109000 | MS.gene21214:CDS |
| GCATAAAGGTTCATTTATCA+AGG | 0.478351 | 5.1:-6108361 | MS.gene21214:CDS |
| GTGGCACTGGCAACACTTGT+AGG | 0.486667 | 5.1:-6108383 | MS.gene21214:CDS |
| ACTATCAAATCACCATATGA+TGG | 0.503242 | 5.1:+6109312 | None:intergenic |
| TGAAATGGATAGTGAATGTC+AGG | 0.523321 | 5.1:+6109432 | None:intergenic |
| AATCTGAAGAAAGCAAAGAA+TGG | 0.524107 | 5.1:-6108951 | MS.gene21214:CDS |
| AACTAGGCATAGGAACTTCA+GGG | 0.534743 | 5.1:+6109070 | None:intergenic |
| CATTTATCAAGGAGGAAAGA+AGG | 0.537861 | 5.1:-6108350 | MS.gene21214:CDS |
| TCTCACACAGCACGCAATAC+AGG | 0.540563 | 5.1:-6108913 | MS.gene21214:intron |
| ACGGTGTAAAGGTGAGGTTA+AGG | 0.543625 | 5.1:+6109214 | None:intergenic |
| ATAGTATTGGAACGGTGTAA+AGG | 0.555489 | 5.1:+6109203 | None:intergenic |
| ACCATATGATGGAAAATGAA+TGG | 0.555827 | 5.1:+6109323 | None:intergenic |
| ATATAAGGGCTATATTTGAA+AGG | 0.590983 | 5.1:+6109018 | None:intergenic |
| GAAGATGAAAACCAAGAAGA+GGG | 0.591458 | 5.1:+6109488 | None:intergenic |
| AGGACAATTGTTGCATAGCG+TGG | 0.602254 | 5.1:-6108330 | MS.gene21214:CDS |
| AAGCTCAAAACCAGAAAGAG+TGG | 0.602365 | 5.1:+6109368 | None:intergenic |
| AGAACGTAATAGTATTGGAA+CGG | 0.607440 | 5.1:+6109195 | None:intergenic |
| GGAAGATGAAAACCAAGAAG+AGG | 0.611027 | 5.1:+6109487 | None:intergenic |
| CAAACCTCTGTTAACTGCAA+CGG | 0.615436 | 5.1:-6109465 | MS.gene21214:CDS |
| ATATTTGAAAGGAATAGCAA+TGG | 0.622554 | 5.1:+6109029 | None:intergenic |
| TAACTTGAATCACTTAAACA+TGG | 0.633744 | 5.1:+6109111 | None:intergenic |
| GTCTTGGTCTTAGATTAACA+TGG | 0.638466 | 5.1:-6108984 | MS.gene21214:CDS |
| AAAGTCTTGTGGAGCAATTG+AGG | 0.638737 | 5.1:+6109157 | None:intergenic |
| TAAAGGTTCATTTATCAAGG+AGG | 0.641975 | 5.1:-6108358 | MS.gene21214:CDS |
| ATTGGAACGGTGTAAAGGTG+AGG | 0.642041 | 5.1:+6109208 | None:intergenic |
| TCTTGGTCTTAGATTAACAT+GGG | 0.645510 | 5.1:-6108983 | MS.gene21214:CDS |
| AATACCGTTGCAGTTAACAG+AGG | 0.663218 | 5.1:+6109461 | None:intergenic |
| TAAGGTTGAGAAAGACTCTG+TGG | 0.692883 | 5.1:+6109232 | None:intergenic |
| ATATGATGGAAAATGAATGG+TGG | 0.729816 | 5.1:+6109326 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTACAAAAATAAAGTTCA+TGG | - | chr5.1:6108964-6108983 | MS.gene21214:CDS | 15.0% |
| !! | ATTACAAAAATAAAGTTCAT+GGG | - | chr5.1:6108965-6108984 | MS.gene21214:CDS | 15.0% |
| !! | GTATAGTACATATATATATT+GGG | - | chr5.1:6109101-6109120 | MS.gene21214:CDS | 15.0% |
| !! | TGTATAGTACATATATATAT+TGG | - | chr5.1:6109100-6109119 | MS.gene21214:CDS | 15.0% |
| !! | AGAAAATTATTAACAAAGCT+TGG | - | chr5.1:6109328-6109347 | MS.gene21214:CDS | 20.0% |
| !! | TTTGTAATTAAAGAAAGAGA+TGG | + | chr5.1:6108953-6108972 | None:intergenic | 20.0% |
| !!! | TTTTAAGAACGTAATAGTAT+TGG | + | chr5.1:6108642-6108661 | None:intergenic | 20.0% |
| ! | AGGAAATTATGCTCATTTAA+AGG | + | chr5.1:6109009-6109028 | None:intergenic | 25.0% |
| ! | AGTACATATATATATTGGGA+AGG | - | chr5.1:6109105-6109124 | MS.gene21214:CDS | 25.0% |
| ! | ATAATTTCCTTATACTAGGT+TGG | - | chr5.1:6109019-6109038 | MS.gene21214:CDS | 25.0% |
| ! | ATATAAGGGCTATATTTGAA+AGG | + | chr5.1:6108814-6108833 | None:intergenic | 25.0% |
| ! | ATATTTGAAAGGAATAGCAA+TGG | + | chr5.1:6108803-6108822 | None:intergenic | 25.0% |
| ! | ATCATTACGAAGTATATACT+AGG | - | chr5.1:6109159-6109178 | MS.gene21214:CDS | 25.0% |
| ! | TAACTTGAATCACTTAAACA+TGG | + | chr5.1:6108721-6108740 | None:intergenic | 25.0% |
| ! | TAATTTCCTTATACTAGGTT+GGG | - | chr5.1:6109020-6109039 | MS.gene21214:CDS | 25.0% |
| !! | ATCAAGAAGATCAATCTTTT+TGG | + | chr5.1:6108563-6108582 | None:intergenic | 25.0% |
| !!! | TTGACTTTTTTACATGAACT+AGG | + | chr5.1:6108778-6108797 | None:intergenic | 25.0% |
| AATCTGAAGAAAGCAAAGAA+TGG | - | chr5.1:6108878-6108897 | MS.gene21214:intron | 30.0% | |
| ACTATCAAATCACCATATGA+TGG | + | chr5.1:6108520-6108539 | None:intergenic | 30.0% | |
| CACTATCCATTTCACATAAA+GGG | - | chr5.1:6108406-6108425 | MS.gene21214:CDS | 30.0% | |
| GAGCATAATTTCCTTATACT+AGG | - | chr5.1:6109015-6109034 | MS.gene21214:CDS | 30.0% | |
| GCATAAAGGTTCATTTATCA+AGG | - | chr5.1:6109468-6109487 | MS.gene21214:CDS | 30.0% | |
| TAAAGGTTCATTTATCAAGG+AGG | - | chr5.1:6109471-6109490 | MS.gene21214:CDS | 30.0% | |
| TACGAAGTATATACTAGGTT+GGG | - | chr5.1:6109164-6109183 | MS.gene21214:CDS | 30.0% | |
| TAGTACATTATCTTTCGTGT+AGG | - | chr5.1:6109226-6109245 | MS.gene21214:CDS | 30.0% | |
| TATATTTCAGACAACAGTCT+TGG | - | chr5.1:6108829-6108848 | MS.gene21214:intron | 30.0% | |
| TCACTATCCATTTCACATAA+AGG | - | chr5.1:6108405-6108424 | MS.gene21214:CDS | 30.0% | |
| TTACGAAGTATATACTAGGT+TGG | - | chr5.1:6109163-6109182 | MS.gene21214:CDS | 30.0% | |
| ! | ACCATATGATGGAAAATGAA+TGG | + | chr5.1:6108509-6108528 | None:intergenic | 30.0% |
| ! | ACCATTCATTTTCCATCATA+TGG | - | chr5.1:6108505-6108524 | MS.gene21214:intron | 30.0% |
| ! | AGAACGTAATAGTATTGGAA+CGG | + | chr5.1:6108637-6108656 | None:intergenic | 30.0% |
| ! | AGACTGTTGTCTGAAATATA+AGG | + | chr5.1:6108829-6108848 | None:intergenic | 30.0% |
| ! | ATATGATGGAAAATGAATGG+TGG | + | chr5.1:6108506-6108525 | None:intergenic | 30.0% |
| ! | GACTGTTGTCTGAAATATAA+GGG | + | chr5.1:6108828-6108847 | None:intergenic | 30.0% |
| ! | TTTTTACATGAACTAGGCAT+AGG | + | chr5.1:6108772-6108791 | None:intergenic | 30.0% |
| !! | TCTTGGTCTTAGATTAACAT+GGG | - | chr5.1:6108846-6108865 | MS.gene21214:intron | 30.0% |
| !! | TTTTGTTTGTGAAAGTCTTG+TGG | + | chr5.1:6108686-6108705 | None:intergenic | 30.0% |
| !!! | ATCAATCTTTTTGGTGTCAT+AGG | + | chr5.1:6108554-6108573 | None:intergenic | 30.0% |
| AAACTACCCAACCTAGTATA+AGG | + | chr5.1:6109029-6109048 | None:intergenic | 35.0% | |
| CATTTATCAAGGAGGAAAGA+AGG | - | chr5.1:6109479-6109498 | MS.gene21214:CDS | 35.0% | |
| GAAGATGAAAACCAAGAAGA+GGG | + | chr5.1:6108344-6108363 | None:intergenic | 35.0% | |
| TATGATGATACCACTCTTTC+TGG | - | chr5.1:6108451-6108470 | MS.gene21214:intron | 35.0% | |
| TCTTTCGTGTAGGATTGATT+CGG | - | chr5.1:6109236-6109255 | MS.gene21214:CDS | 35.0% | |
| TGAAATGGATAGTGAATGTC+AGG | + | chr5.1:6108400-6108419 | None:intergenic | 35.0% | |
| TGTTGACCCTTTATGTGAAA+TGG | + | chr5.1:6108415-6108434 | None:intergenic | 35.0% | |
| ! | ATAGTATTGGAACGGTGTAA+AGG | + | chr5.1:6108629-6108648 | None:intergenic | 35.0% |
| ! | ATATACTAGGTTGGGTAGTT+TGG | - | chr5.1:6109172-6109191 | MS.gene21214:CDS | 35.0% |
| !! | GTCTTGGTCTTAGATTAACA+TGG | - | chr5.1:6108845-6108864 | MS.gene21214:intron | 35.0% |
| !!! | CTTTTTGGTGTCATAGGAAA+TGG | + | chr5.1:6108548-6108567 | None:intergenic | 35.0% |
| AACTAGGCATAGGAACTTCA+GGG | + | chr5.1:6108762-6108781 | None:intergenic | 40.0% | |
| AAGCTCAAAACCAGAAAGAG+TGG | + | chr5.1:6108464-6108483 | None:intergenic | 40.0% | |
| AATACCGTTGCAGTTAACAG+AGG | + | chr5.1:6108371-6108390 | None:intergenic | 40.0% | |
| CAAACCTCTGTTAACTGCAA+CGG | - | chr5.1:6108364-6108383 | MS.gene21214:CDS | 40.0% | |
| GGAAGATGAAAACCAAGAAG+AGG | + | chr5.1:6108345-6108364 | None:intergenic | 40.0% | |
| TAAGGTTGAGAAAGACTCTG+TGG | + | chr5.1:6108600-6108619 | None:intergenic | 40.0% | |
| TTCAACACATGTGCAGTTCT+TGG | - | chr5.1:6109401-6109420 | MS.gene21214:CDS | 40.0% | |
| ! | AAAGTCTTGTGGAGCAATTG+AGG | + | chr5.1:6108675-6108694 | None:intergenic | 40.0% |
| ! | TCTCAGCATCTGTATTTTCG+TGG | - | chr5.1:6109424-6109443 | MS.gene21214:CDS | 40.0% |
| !!! | ACTCTGTGGACACAGTTTTT+TGG | + | chr5.1:6108586-6108605 | None:intergenic | 40.0% |
| ACGGTGTAAAGGTGAGGTTA+AGG | + | chr5.1:6108618-6108637 | None:intergenic | 45.0% | |
| AGGACAATTGTTGCATAGCG+TGG | - | chr5.1:6109499-6109518 | MS.gene21214:CDS | 45.0% | |
| ATTGGAACGGTGTAAAGGTG+AGG | + | chr5.1:6108624-6108643 | None:intergenic | 45.0% | |
| CGTTGCAGTTAACAGAGGTT+TGG | + | chr5.1:6108366-6108385 | None:intergenic | 45.0% | |
| CTTCTTTCTCACCCTCTTCT+TGG | - | chr5.1:6108330-6108349 | MS.gene21214:CDS | 45.0% | |
| GAACTAGGCATAGGAACTTC+AGG | + | chr5.1:6108763-6108782 | None:intergenic | 45.0% | |
| GGCAACACTTGTAGGCATAA+AGG | - | chr5.1:6109454-6109473 | MS.gene21214:CDS | 45.0% | |
| GTTAACTGCAACGGTATTGC+TGG | - | chr5.1:6108373-6108392 | MS.gene21214:CDS | 45.0% | |
| ! | CAGCATCTGTATTTTCGTGG+TGG | - | chr5.1:6109427-6109446 | MS.gene21214:CDS | 45.0% |
| TCTCACACAGCACGCAATAC+AGG | - | chr5.1:6108916-6108935 | MS.gene21214:CDS | 50.0% | |
| ! | CTGTATTTTCGTGGTGGCAC+TGG | - | chr5.1:6109433-6109452 | MS.gene21214:CDS | 50.0% |
| !! | GTGGCACTGGCAACACTTGT+AGG | - | chr5.1:6109446-6109465 | MS.gene21214:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 6108319 | 6109532 | 6108319 | ID=MS.gene21214 |
| chr5.1 | mRNA | 6108319 | 6109532 | 6108319 | ID=MS.gene21214.t1;Parent=MS.gene21214 |
| chr5.1 | exon | 6108914 | 6109532 | 6108914 | ID=MS.gene21214.t1.exon1;Parent=MS.gene21214.t1 |
| chr5.1 | CDS | 6108914 | 6109532 | 6108914 | ID=cds.MS.gene21214.t1;Parent=MS.gene21214.t1 |
| chr5.1 | exon | 6108319 | 6108434 | 6108319 | ID=MS.gene21214.t1.exon2;Parent=MS.gene21214.t1 |
| chr5.1 | CDS | 6108319 | 6108434 | 6108319 | ID=cds.MS.gene21214.t1;Parent=MS.gene21214.t1 |
| Gene Sequence |
| Protein sequence |
