AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050769


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050769.t1 MTR_2g097410 87.368 95 12 0 26 120 336 430 2.23e-50 174
MS.gene050769.t1 MTR_4g064150 62.667 75 28 0 40 114 340 414 6.92e-28 110
MS.gene050769.t1 MTR_3g087300 92.727 55 4 0 26 80 154 208 7.66e-28 105
MS.gene050769.t1 MTR_4g064160 56.180 89 39 0 30 118 285 373 4.50e-27 108
MS.gene050769.t1 MTR_4g064120 49.505 101 48 2 21 119 215 314 6.69e-25 102
MS.gene050769.t1 MTR_7g062120 59.211 76 31 0 43 118 354 429 1.08e-24 101
MS.gene050769.t1 MTR_4g064180 60.000 70 28 0 42 111 235 304 6.37e-23 96.7
MS.gene050769.t1 MTR_2g097390 49.425 87 44 0 32 118 246 332 5.68e-21 90.9
MS.gene050769.t1 MTR_2g097350 52.778 72 34 0 47 118 260 331 5.52e-20 88.2
MS.gene050769.t1 MTR_2g097310 54.930 71 29 1 51 118 259 329 5.20e-19 85.5
MS.gene050769.t1 MTR_2g097463 56.522 69 28 1 41 107 268 336 8.65e-17 79.0
MS.gene050769.t1 MTR_4g064200 56.923 65 27 1 56 120 279 342 1.32e-15 75.5
MS.gene050769.t1 MTR_2g097467 41.322 121 58 6 15 124 236 354 2.03e-15 74.7
MS.gene050769.t1 MTR_2g097473 41.964 112 55 4 15 118 236 345 1.68e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050769.t1 AT1G07530 54.651 86 32 1 40 118 368 453 4.16e-23 97.4
MS.gene050769.t1 AT1G07520 50.549 91 39 2 25 113 284 370 5.98e-20 88.2
MS.gene050769.t1 AT1G07520 50.549 91 39 2 25 113 285 371 5.98e-20 88.2
MS.gene050769.t1 AT2G29060 51.282 78 38 0 41 118 301 378 9.27e-20 87.4
MS.gene050769.t1 AT1G07520 49.474 95 36 3 24 113 119 206 1.42e-19 87.0
MS.gene050769.t1 AT1G07520 49.474 95 36 3 24 113 119 206 1.42e-19 87.0
MS.gene050769.t1 AT2G29060 51.282 78 38 0 41 118 264 341 3.19e-19 85.9
MS.gene050769.t1 AT2G37650 55.714 70 31 0 49 118 335 404 1.91e-18 84.0
MS.gene050769.t1 AT2G37650 55.714 70 31 0 49 118 323 392 2.25e-18 83.6
MS.gene050769.t1 AT2G29065 57.627 59 25 0 55 113 246 304 6.80e-14 70.5
MS.gene050769.t1 AT3G46600 38.144 97 59 1 42 138 161 256 1.30e-13 69.7
MS.gene050769.t1 AT3G46600 38.144 97 59 1 42 138 193 288 1.60e-13 69.3
MS.gene050769.t1 AT5G59450 45.333 75 38 1 41 112 204 278 3.52e-13 68.2
MS.gene050769.t1 AT3G46600 39.506 81 48 1 58 138 1 80 4.63e-11 62.0

Find 0 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATACAAAAAAAAAATGAA+TGG - 51772:1389-1408 MS.gene050769:intron 15.0%
!! TGAACATAAATAATTATAAC+TGG + 51772:1899-1918 None:intergenic 15.0%
!! ATCAAGTTTGTAAACTATAA+TGG - 51772:499-518 MS.gene050769:intron 20.0%
!! ATCCTTAATCAATATCAAAA+GGG + 51772:2163-2182 None:intergenic 20.0%
!!! AACATTTTTAGCAAATTTTC+AGG + 51772:644-663 None:intergenic 20.0%
!!! TAATGTTAAAACTTTTTAGC+AGG + 51772:384-403 None:intergenic 20.0%
!!! TACTATGTTTTCCTTAATTT+TGG - 51772:1774-1793 MS.gene050769:intron 20.0%
!!! TTAAAATGTTCTTTGAGTTT+TGG + 51772:338-357 None:intergenic 20.0%
!!! TTGTATAGAATATTTTGTGT+TGG - 51772:1630-1649 MS.gene050769:CDS 20.0%
! CATCCTTAATCAATATCAAA+AGG + 51772:2164-2183 None:intergenic 25.0%
! GCTAATAAAGTTATTGCATT+TGG - 51772:197-216 MS.gene050769:CDS 25.0%
! GTACAGAAATTTGAATTCAT+TGG - 51772:700-719 MS.gene050769:intron 25.0%
! GTCTTATAAGCTAACTTTAT+TGG - 51772:1201-1220 MS.gene050769:intron 25.0%
! TGCATTCAACTTAAGATATT+TGG - 51772:1585-1604 MS.gene050769:CDS 25.0%
! TTTATGCATGTCAATATGAA+TGG - 51772:2015-2034 MS.gene050769:intron 25.0%
!! AATTTGGATGCTTTATTAAC+AGG - 51772:1673-1692 MS.gene050769:intron 25.0%
!! ACATCAGTTTTTAACAACTT+AGG + 51772:434-453 None:intergenic 25.0%
!! GTTAATAAAGCATCCAAATT+TGG + 51772:1673-1692 None:intergenic 25.0%
!! GTTAGTTTAGACCAAAATTA+AGG + 51772:1788-1807 None:intergenic 25.0%
!! TTTTCTTTTTGTAAGTTGCA+TGG + 51772:726-745 None:intergenic 25.0%
!!! ATAGTCTTGGTTAACTTTTT+TGG - 51772:598-617 MS.gene050769:CDS 25.0%
!!! CAACCATTTTTTTTATAAGC+AGG + 51772:1613-1632 None:intergenic 25.0%
!!! TAATTTTGGTCTAAACTAAC+AGG - 51772:1788-1807 MS.gene050769:intron 25.0%
!!! TACCCTTTTGATATTGATTA+AGG - 51772:2158-2177 MS.gene050769:intron 25.0%
!!! TAGAATATTTTGTGTTGGTT+TGG - 51772:1635-1654 MS.gene050769:CDS 25.0%
!!! TGCAGGAATTTTGATAATAT+TGG - 51772:1263-1282 MS.gene050769:intron 25.0%
!!! TTAATTGTTTTTCAGACTCA+TGG - 51772:2184-2203 MS.gene050769:intron 25.0%
!!! TTTGTGATTTTGATTGCAAA+AGG - 51772:1106-1125 MS.gene050769:CDS 25.0%
AAGTCACAAGTAACAACAAT+AGG - 51772:1947-1966 MS.gene050769:intron 30.0%
ACATAGTAAATAAGTCAGCA+AGG + 51772:1762-1781 None:intergenic 30.0%
ACTTCTATCAAAATTAGCAG+TGG - 51772:1314-1333 MS.gene050769:intron 30.0%
ATGAATTGTTGAAGCAGATT+AGG - 51772:928-947 MS.gene050769:intron 30.0%
CAAGTAACAACAATAGGTTA+TGG - 51772:1953-1972 MS.gene050769:intron 30.0%
GAGCCTGCTTATAAAAAAAA+TGG - 51772:1607-1626 MS.gene050769:CDS 30.0%
GCTGATTAGAATACCAAATT+TGG - 51772:1657-1676 MS.gene050769:CDS 30.0%
GGCTCATTGTTACAATATAT+TGG - 51772:1525-1544 MS.gene050769:CDS 30.0%
TAGTAGTTAAAGAGAAGCTT+TGG - 51772:280-299 MS.gene050769:intron 30.0%
TCTGATGTTTAAGAATTTGC+TGG - 51772:574-593 MS.gene050769:intron 30.0%
TGAACTTTAACAACCTCTTA+TGG - 51772:1504-1523 MS.gene050769:CDS 30.0%
TTGCTGATCAATTGAATAAC+AGG - 51772:1424-1443 MS.gene050769:CDS 30.0%
! AATCAGCACTAAACATCTTT+TGG + 51772:1067-1086 None:intergenic 30.0%
! CTGATGTAATGATAGTTTGT+TGG - 51772:447-466 MS.gene050769:intron 30.0%
! GGAATAGAAGTTTTACATAG+AGG - 51772:1445-1464 MS.gene050769:CDS 30.0%
! TGGTAGAGTCATGATTATTT+TGG - 51772:467-486 MS.gene050769:intron 30.0%
AGTAGCTAGAATGAAAGTTG+AGG - 51772:1724-1743 MS.gene050769:intron 35.0%
GTTATTGCATTTGGCTGTAT+TGG - 51772:206-225 MS.gene050769:CDS 35.0%
TTGTAACAATGAGCCATAAG+AGG + 51772:1520-1539 None:intergenic 35.0%
! TAGTTAAAGAGAAGCTTTGG+AGG - 51772:283-302 MS.gene050769:intron 35.0%
! TTTGATAATATTGGCAGCGT+TGG - 51772:1272-1291 MS.gene050769:CDS 35.0%
!! AGAATTTGCTGGTATAGTCT+TGG - 51772:585-604 MS.gene050769:CDS 35.0%
!! GTAAACTATAATGGTGCCAT+TGG - 51772:508-527 MS.gene050769:intron 35.0%
!!! AGTGCTGATTTTGATTGTGT+AGG - 51772:1077-1096 MS.gene050769:CDS 35.0%
AAGGTATGTTAGCATGGGTT+CGG - 51772:1367-1386 MS.gene050769:intron 40.0%
ACCTTTCAGCATCATAGAGA+TGG + 51772:1171-1190 None:intergenic 40.0%
ATGCAACCAAAGTGATCCAA+TGG + 51772:527-546 None:intergenic 40.0%
CAATAGGTTATGGTCAACAG+TGG - 51772:1963-1982 MS.gene050769:intron 40.0%
CTGTGTCTGCTAATGACAAT+AGG - 51772:898-917 MS.gene050769:intron 40.0%
TCAAGCCAATCAAACTACAG+TGG - 51772:1834-1853 MS.gene050769:intron 40.0%
TCCATCTCTATGATGCTGAA+AGG - 51772:1167-1186 MS.gene050769:CDS 40.0%
TGAACAAAAGGATGGATCAG+TGG - 51772:761-780 MS.gene050769:intron 40.0%
TTGGATCACTTTGGTTGCAT+AGG - 51772:527-546 MS.gene050769:intron 40.0%
TTGTGCACACAGAACCAAAA+GGG + 51772:877-896 None:intergenic 40.0%
TTGTGTGCTGAACAAAAGGA+TGG - 51772:753-772 MS.gene050769:intron 40.0%
! GCTGAAAGGTATGTTAGCAT+GGG - 51772:1362-1381 MS.gene050769:intron 40.0%
! TCCTTTTGTTCAGCACACAA+CGG + 51772:753-772 None:intergenic 40.0%
! TGCTGAAAGGTATGTTAGCA+TGG - 51772:1361-1380 MS.gene050769:intron 40.0%
AAATGCGATTGAAGCGCACA+TGG - 51772:1004-1023 MS.gene050769:intron 45.0%
ACAAAAGGATGGATCAGTGG+TGG - 51772:764-783 MS.gene050769:intron 45.0%
ACCTTTCAGCACTACAACTG+AGG + 51772:1352-1371 None:intergenic 45.0%
AGAGGGATGTTGTGGATTTG+AGG - 51772:850-869 MS.gene050769:intron 45.0%
AGCCAATCAAACTACAGTGG+TGG - 51772:1837-1856 MS.gene050769:intron 45.0%
AGGTGATGCATCAGAGAGAA+TGG - 51772:968-987 MS.gene050769:intron 45.0%
CACCACCACTGTAGTTTGAT+TGG + 51772:1842-1861 None:intergenic 45.0%
CTTGTGCACACAGAACCAAA+AGG + 51772:878-897 None:intergenic 45.0%
GAAGAAACAAGGGAGGAAGA+GGG - 51772:833-852 MS.gene050769:intron 45.0%
GGGTACATTGCACAAAGACA+CGG + 51772:2143-2162 None:intergenic 45.0%
GGTACATTGCACAAAGACAC+GGG + 51772:2142-2161 None:intergenic 45.0%
GTATGAATGTACGACTGTGC+AGG - 51772:1246-1265 MS.gene050769:intron 45.0%
TAGGTTATGGTCAACAGTGG+AGG - 51772:1966-1985 MS.gene050769:intron 45.0%
TATGGTCAACAGTGGAGGTA+GGG - 51772:1971-1990 MS.gene050769:intron 45.0%
TGGAGGTAGGGCTAAAGATT+AGG - 51772:1983-2002 MS.gene050769:intron 45.0%
TTATGGTCAACAGTGGAGGT+AGG - 51772:1970-1989 MS.gene050769:intron 45.0%
! AGTTACTTTCTTGCCTCAGG+GGG - 51772:1130-1149 MS.gene050769:CDS 45.0%
! CAGTTACTTTCTTGCCTCAG+GGG - 51772:1129-1148 MS.gene050769:CDS 45.0%
! GATGTTTTTCCTCTTGCAGC+TGG + 51772:1816-1835 None:intergenic 45.0%
! GCAGTTACTTTCTTGCCTCA+GGG - 51772:1128-1147 MS.gene050769:CDS 45.0%
! TCCTCAGTTGTAGTGCTGAA+AGG - 51772:1348-1367 MS.gene050769:intron 45.0%
! TGTGGATTTGAGGACCCTTT+TGG - 51772:860-879 MS.gene050769:intron 45.0%
!! ACACAGGTTGGTGAACTTGA+TGG - 51772:795-814 MS.gene050769:intron 45.0%
!! AGGAAGCATTCTTGTCCTTC+AGG - 51772:948-967 MS.gene050769:intron 45.0%
!! AGGTTGGTGAACTTGATGGA+GGG - 51772:799-818 MS.gene050769:intron 45.0%
!! ATGGTGCCATTGGATCACTT+TGG - 51772:518-537 MS.gene050769:intron 45.0%
!! TGGCAGCGTTGGTAATCATT+CGG - 51772:1283-1302 MS.gene050769:CDS 45.0%
AAGGCGCGTTCGAAGAAACA+AGG - 51772:822-841 MS.gene050769:intron 50.0%
AGGCGCGTTCGAAGAAACAA+GGG - 51772:823-842 MS.gene050769:intron 50.0%
CGAAGAAACAAGGGAGGAAG+AGG - 51772:832-851 MS.gene050769:intron 50.0%
CTCTCTGATGCATCACCTGA+AGG + 51772:966-985 None:intergenic 50.0%
CTTGCAATCACAGCTCCCAA+AGG + 51772:2244-2263 None:intergenic 50.0%
GCCGTTGTGTGCTGAACAAA+AGG - 51772:749-768 MS.gene050769:intron 50.0%
GTGGAGAGTAGAACACAGGT+TGG - 51772:783-802 MS.gene050769:intron 50.0%
TAACAGGAACCAGCTGCAAG+AGG - 51772:1804-1823 MS.gene050769:intron 50.0%
TGGTGAACTTGATGGAGGGA+AGG - 51772:803-822 MS.gene050769:intron 50.0%
! AGTGCTGAATGCAGTGAGCT+GGG - 51772:2207-2226 MS.gene050769:CDS 50.0%
! AGTGGTGGAGAGTAGAACAC+AGG - 51772:779-798 MS.gene050769:intron 50.0%
! GGCAGTTACTTTCTTGCCTC+AGG - 51772:1127-1146 MS.gene050769:CDS 50.0%
!! CACATGGTTGGTGCTGGTAA+CGG - 51772:1020-1039 MS.gene050769:intron 50.0%
!! CAGGTTGGTGAACTTGATGG+AGG - 51772:798-817 MS.gene050769:intron 50.0%
AGGGAGGAAGAGGGATGTTG+TGG - 51772:842-861 MS.gene050769:intron 55.0%
CATACAATCTCGTCCCCCTG+AGG + 51772:1146-1165 None:intergenic 55.0%
CGCGTTCGAAGAAACAAGGG+AGG - 51772:826-845 MS.gene050769:intron 55.0%
GCGATTGAAGCGCACATGGT+TGG - 51772:1008-1027 MS.gene050769:intron 55.0%
GTGCTGAATGCAGTGAGCTG+GGG - 51772:2208-2227 MS.gene050769:CDS 55.0%
TGCTGAATGCAGTGAGCTGG+GGG - 51772:2209-2228 MS.gene050769:CDS 55.0%
TGGGGGACCTTGATGCCTTT+GGG - 51772:2226-2245 MS.gene050769:CDS 55.0%
!! CACAGCTCCCAAAGGCATCA+AGG + 51772:2236-2255 None:intergenic 55.0%
!! GAGTGCTGAATGCAGTGAGC+TGG - 51772:2206-2225 MS.gene050769:CDS 55.0%
CTGGGGGACCTTGATGCCTT+TGG - 51772:2225-2244 MS.gene050769:CDS 60.0%
! GAAGCGCACATGGTTGGTGC+TGG - 51772:1014-1033 MS.gene050769:intron 60.0%


Chromosome Type Strat End Strand Name
51772 gene 195 2263 195 ID=MS.gene050769
51772 mRNA 195 2263 195 ID=MS.gene050769.t1;Parent=MS.gene050769
51772 exon 2189 2263 2189 ID=MS.gene050769.t1.exon1;Parent=MS.gene050769.t1
51772 CDS 2189 2263 2189 ID=cds.MS.gene050769.t1;Parent=MS.gene050769.t1
51772 exon 1417 1687 1417 ID=MS.gene050769.t1.exon2;Parent=MS.gene050769.t1
51772 CDS 1417 1687 1417 ID=cds.MS.gene050769.t1;Parent=MS.gene050769.t1
51772 exon 1270 1330 1270 ID=MS.gene050769.t1.exon3;Parent=MS.gene050769.t1
51772 CDS 1270 1330 1270 ID=cds.MS.gene050769.t1;Parent=MS.gene050769.t1
51772 exon 1070 1190 1070 ID=MS.gene050769.t1.exon4;Parent=MS.gene050769.t1
51772 CDS 1070 1190 1070 ID=cds.MS.gene050769.t1;Parent=MS.gene050769.t1
51772 exon 583 648 583 ID=MS.gene050769.t1.exon5;Parent=MS.gene050769.t1
51772 CDS 583 648 583 ID=cds.MS.gene050769.t1;Parent=MS.gene050769.t1
51772 exon 195 259 195 ID=MS.gene050769.t1.exon6;Parent=MS.gene050769.t1
51772 CDS 195 259 195 ID=cds.MS.gene050769.t1;Parent=MS.gene050769.t1
Gene Sequence

>MS.gene050769

ATGCTAATAAAGTTATTGCATTTGGCTGTATTGGTGACATATATCAGTGATGAATTTGTTGCTCTATATCACTGTGTAAGAAGTTTAGTAGTTAAAGAGAAGCTTTGGAGGAAGTTTAGCAGAAAGTAATTTTGTTAGTGCCAAAACTCAAAGAACATTTTAAAGATACTTAGATGCATGATTTTACCTGCTAAAAAGTTTTAACATTATATTTGAAAGTGACAGATGCAGTTGTGCCTAAGTTGTTAAAAACTGATGTAATGATAGTTTGTTGGTAGAGTCATGATTATTTTGGTTAAAATCAATCAAGTTTGTAAACTATAATGGTGCCATTGGATCACTTTGGTTGCATAGGATTGATTGATTTGAATTGTTAGAATCTGATGTTTAAGAATTTGCTGGTATAGTCTTGGTTAACTTTTTTGGATACATTAAAGTAATTGTTACCTGAAAATTTGCTAAAAATGTTAAGTTCTTGTGATCTGATTGCTGAGTTTGAGAGCATGTACAGAAATTTGAATTCATTGGCCATGCAACTTACAAAAAGAAAATGTGCCGTTGTGTGCTGAACAAAAGGATGGATCAGTGGTGGAGAGTAGAACACAGGTTGGTGAACTTGATGGAGGGAAGGCGCGTTCGAAGAAACAAGGGAGGAAGAGGGATGTTGTGGATTTGAGGACCCTTTTGGTTCTGTGTGCACAAGCTGTGTCTGCTAATGACAATAGGACTGATAATGAATTGTTGAAGCAGATTAGGAAGCATTCTTGTCCTTCAGGTGATGCATCAGAGAGAATGGCTCATTACTTTGCAAATGCGATTGAAGCGCACATGGTTGGTGCTGGTAACGGTACACAAATATTATATATGAGCCAAAAGATGTTTAGTGCTGATTTTGATTGTGTAGGTATGAATTTGTGATTTTGATTGCAAAAGGCAGTTACTTTCTTGCCTCAGGGGGACGAGATTGTATGATCCATCTCTATGATGCTGAAAGGTGTAGTTATCAGTCTTATAAGCTAACTTTATTGGCTGCTTGTGCATTTTGATGACTGTATGAATGTACGACTGTGCAGGAATTTTGATAATATTGGCAGCGTTGGTAATCATTCGGCTGCAGTGACTTCTATCAAAATTAGCAGTGGCAGTTGCAAGATCCTCAGTTGTAGTGCTGAAAGGTATGTTAGCATGGGTTCGGTATACAAAAAAAAAATGAATGGACTGTGTATGTTTTGCTGATCAATTGAATAACAGGAATAGAAGTTTTACATAGAGGTTCTGACTTTTTTGTGTAAAAAACTACAAATGAATTTGAACTTTAACAACCTCTTATGGCTCATTGTTACAATATATTGGTTTAGTTTTTTCATATGTACATGTGTTACAATGATTGTGCATTCAACTTAAGATATTTGGAGCCTGCTTATAAAAAAAATGGTTGTATAGAATATTTTGTGTTGGTTTGGCTGATTAGAATACCAAATTTGGATGCTTTATTAACAGGAAATTCATGATATGCTAAACTTTGAGAAAGTAGCTAGAATGAAAGTTGAGGACATAACTTATTCCTTGCTGACTTATTTACTATGTTTTCCTTAATTTTGGTCTAAACTAACAGGAACCAGCTGCAAGAGGAAAAACATCAAGCCAATCAAACTACAGTGGTGGTGCATTTTGCACAACAGTGCGTTTATTAATCAAACACCAGTTATAATTATTTATGTTCATGAAACTGATTGCTTTTTGAAAATTTAGAAGTCACAAGTAACAACAATAGGTTATGGTCAACAGTGGAGGTAGGGCTAAAGATTAGGAGTACAATATTTATGCATGTCAATATGAATGGTACACAAAAGTATTTGAGAAAGTTCTAAATTTATATTCAGAATCATAAAATCATATGATATTTTAATTTTAATGAATGACGATGCATAGATTTTAAAATCTCCCGTGTCTTTGTGCAATGTACCCTTTTGATATTGATTAAGGATGTTAATTGTTTTTCAGACTCATGGAGTGCTGAATGCAGTGAGCTGGGGGACCTTGATGCCTTTGGGAGCTGTGATTGCAAG

Protein sequence

>MS.gene050769.t1

MLIKLLHLAVLVTYISDEFVALYHCDGSVVESRTQVGELDGGKARSKKQGRKRDVVDLRTLLVLCAQAVSANDNRTDNELLKQIRKHSCPSGDASERMAHYFANAIEAHMVGAGNGSYFLASGGRDCMIHLYDAERNFDNIGSVGNHSAAVTSIKISSGSCKILSCSAERYVSMGSEPAARGKTSSQSNYSGGAFCTTTHGVLNAVSWGTLMPLGAVIA