Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050803.t1 | XP_003626129.2 | 86 | 449 | 59 | 2 | 1 | 446 | 1 | 448 | 5.60E-209 | 736.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050803.t1 | G7KVK4 | 86.0 | 449 | 59 | 2 | 1 | 446 | 1 | 448 | 4.1e-209 | 736.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050803 | MS.gene050966 | 0.985981 | 2.74E-165 | -1.69E-46 |
| MS.gene050803 | MS.gene072604 | 0.848739 | 5.06E-60 | -1.69E-46 |
| MS.gene050803 | MS.gene072605 | 0.853416 | 2.42E-61 | -1.69E-46 |
| MS.gene050803 | MS.gene22786 | 0.854381 | 1.28E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050803.t1 | MTR_7g111320 | 91.295 | 448 | 35 | 2 | 1 | 445 | 1 | 447 | 0.0 | 828 |
| MS.gene050803.t1 | MTR_1g084980 | 47.336 | 488 | 204 | 11 | 2 | 446 | 1 | 478 | 3.43e-126 | 377 |
| MS.gene050803.t1 | MTR_1g084980 | 47.336 | 488 | 204 | 11 | 2 | 446 | 1 | 478 | 8.37e-125 | 379 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050803.t1 | AT1G09530 | 25.664 | 452 | 230 | 13 | 2 | 446 | 1 | 353 | 2.50e-14 | 75.5 |
| MS.gene050803.t1 | AT1G09530 | 25.664 | 452 | 230 | 13 | 2 | 446 | 1 | 353 | 2.50e-14 | 75.5 |
| MS.gene050803.t1 | AT1G09530 | 25.664 | 452 | 230 | 13 | 2 | 446 | 1 | 353 | 2.50e-14 | 75.5 |
| MS.gene050803.t1 | AT1G09530 | 25.664 | 452 | 230 | 13 | 2 | 446 | 1 | 353 | 2.50e-14 | 75.5 |
| MS.gene050803.t1 | AT1G09530 | 25.664 | 452 | 230 | 13 | 2 | 446 | 1 | 353 | 2.50e-14 | 75.5 |
| MS.gene050803.t1 | AT1G09530 | 25.664 | 452 | 230 | 13 | 2 | 446 | 1 | 353 | 2.50e-14 | 75.5 |
Find 0 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAATCTTTTAAAAAATCTA+CGG | + | 50606:16768-16787 | MS.gene050803:CDS | 15.0% |
| !! | AAGCAAAGAAAATTTATTTG+TGG | - | 50606:16122-16141 | MS.gene050803:intergenic | 20.0% |
| !! | GTTTGAATGAAATTATTACA+AGG | + | 50606:15957-15976 | MS.gene050803:CDS | 20.0% |
| !!! | AAATCTAATTGATTCAAATG+AGG | - | 50606:16302-16321 | MS.gene050803:intergenic | 20.0% |
| !!! | AATCTAATTGATTCAAATGA+GGG | - | 50606:16301-16320 | MS.gene050803:intergenic | 20.0% |
| !!! | CAATTTTTTTGTTAACTTGT+AGG | + | 50606:16993-17012 | MS.gene050803:intron | 20.0% |
| ! | AAAATGGGTGAAATTCATTA+CGG | - | 50606:16416-16435 | MS.gene050803:intergenic | 25.0% |
| ! | AAGTTTGAGGATTTAGATAA+CGG | + | 50606:15935-15954 | MS.gene050803:CDS | 25.0% |
| ! | AATGAAACAAATACAAGGAT+AGG | + | 50606:15911-15930 | MS.gene050803:CDS | 25.0% |
| ! | ACACAATTTCTAACACTAAT+GGG | - | 50606:15677-15696 | MS.gene050803:intergenic | 25.0% |
| ! | ATGCTAATGAAACAAATACA+AGG | + | 50606:15906-15925 | MS.gene050803:CDS | 25.0% |
| ! | CACAAGATAATTCAACAAAT+AGG | - | 50606:16940-16959 | MS.gene050803:intergenic | 25.0% |
| ! | TACACAATTTCTAACACTAA+TGG | - | 50606:15678-15697 | MS.gene050803:intergenic | 25.0% |
| ! | TCAATTAGATTTAGAGAATC+AGG | + | 50606:16310-16329 | MS.gene050803:CDS | 25.0% |
| ! | TTAGGTGTGAATAATACAAA+AGG | + | 50606:16334-16353 | MS.gene050803:CDS | 25.0% |
| !! | AAATTTGAAGCGAAAAAATC+TGG | + | 50606:16861-16880 | MS.gene050803:CDS | 25.0% |
| !! | TCATCAAAAGTTGATTTAAG+TGG | + | 50606:16556-16575 | MS.gene050803:CDS | 25.0% |
| !!! | GATTTTGAAGATGAATCAAT+TGG | + | 50606:17015-17034 | MS.gene050803:CDS | 25.0% |
| AAAGCTAATCTTCAGAACAT+TGG | + | 50606:16457-16476 | MS.gene050803:CDS | 30.0% | |
| AACTGAAACAAATCGTTTCT+GGG | - | 50606:15776-15795 | MS.gene050803:intergenic | 30.0% | |
| AAGGGTCATGATAAATATTC+AGG | + | 50606:15878-15897 | MS.gene050803:CDS | 30.0% | |
| AGATTTAGAGAATCAGGTTT+AGG | + | 50606:16316-16335 | MS.gene050803:CDS | 30.0% | |
| ATATGAGAGATTTCTTCACA+AGG | - | 50606:16379-16398 | MS.gene050803:intergenic | 30.0% | |
| ATTGGAAGTTTGATCAACTT+TGG | - | 50606:16716-16735 | MS.gene050803:intergenic | 30.0% | |
| TTTAGTGACTCTCATAAACA+AGG | + | 50606:16169-16188 | MS.gene050803:CDS | 30.0% | |
| ! | GTGTTTTTCTCAAGAGATTT+CGG | - | 50606:16658-16677 | MS.gene050803:intergenic | 30.0% |
| !! | AGTTGAAGATTTTGATACCT+CGG | + | 50606:16219-16238 | MS.gene050803:CDS | 30.0% |
| !! | CAACTTTTTCTTTAGACATG+TGG | - | 50606:15593-15612 | MS.gene050803:intergenic | 30.0% |
| AAGTGCACAATCTATCTGAA+AGG | + | 50606:17097-17116 | MS.gene050803:CDS | 35.0% | |
| ACTTACACAGATGATTGATC+TGG | - | 50606:15645-15664 | MS.gene050803:intergenic | 35.0% | |
| AGTCCATCTCATACTCTTAA+GGG | + | 50606:15860-15879 | MS.gene050803:CDS | 35.0% | |
| ATTGACCATGCACTAAAATC+TGG | - | 50606:15814-15833 | MS.gene050803:intergenic | 35.0% | |
| CAAACTTCCAATCAAGTTCT+TGG | + | 50606:16724-16743 | MS.gene050803:CDS | 35.0% | |
| CAACTGAAACAAATCGTTTC+TGG | - | 50606:15777-15796 | MS.gene050803:intergenic | 35.0% | |
| CACTATTTACATCGTAGTTC+TGG | + | 50606:16052-16071 | MS.gene050803:CDS | 35.0% | |
| CGACGGCATATTTAATTGAT+TGG | - | 50606:16260-16279 | MS.gene050803:intergenic | 35.0% | |
| CTTACACAGATGATTGATCT+GGG | - | 50606:15644-15663 | MS.gene050803:intergenic | 35.0% | |
| GGTGTTAAAAGAACTGATCA+TGG | + | 50606:17036-17055 | MS.gene050803:CDS | 35.0% | |
| GGTTTCTTACATGAACTATC+TGG | + | 50606:16073-16092 | MS.gene050803:CDS | 35.0% | |
| GTTGAATTATCTTGTGAGTG+TGG | + | 50606:16944-16963 | MS.gene050803:intron | 35.0% | |
| TACAAGGATAGGAAAGTTTG+AGG | + | 50606:15922-15941 | MS.gene050803:CDS | 35.0% | |
| TTACACAGATGATTGATCTG+GGG | - | 50606:15643-15662 | MS.gene050803:intergenic | 35.0% | |
| TTCAGATAGATTGTGCACTT+CGG | - | 50606:17097-17116 | MS.gene050803:intergenic | 35.0% | |
| ! | AAACAAGGTATCCTTTCCAA+GGG | + | 50606:16184-16203 | MS.gene050803:CDS | 35.0% |
| ! | ACGATTTGTTTCAGTTGGTT+TGG | + | 50606:15780-15799 | MS.gene050803:CDS | 35.0% |
| ! | CAATGTATGGCTGAACTTTT+TGG | - | 50606:16589-16608 | MS.gene050803:intergenic | 35.0% |
| ! | CAGAAACGATTTGTTTCAGT+TGG | + | 50606:15775-15794 | MS.gene050803:CDS | 35.0% |
| ! | TAAACAAGGTATCCTTTCCA+AGG | + | 50606:16183-16202 | MS.gene050803:CDS | 35.0% |
| ! | TCATCTTGACTTGAAGAAAC+AGG | - | 50606:15983-16002 | MS.gene050803:intergenic | 35.0% |
| !! | AATGACCAGATTTTAGTGCA+TGG | + | 50606:15806-15825 | MS.gene050803:CDS | 35.0% |
| !! | GTTGAAGATTTTGATACCTC+GGG | + | 50606:16220-16239 | MS.gene050803:CDS | 35.0% |
| !! | TCAACTTTTGATGATTCGCA+AGG | - | 50606:16550-16569 | MS.gene050803:intergenic | 35.0% |
| AAAAGAACTGATCATGGACG+AGG | + | 50606:17042-17061 | MS.gene050803:CDS | 40.0% | |
| AACAAAGATGCAGCTGTGAT+AGG | + | 50606:16517-16536 | MS.gene050803:CDS | 40.0% | |
| AACTGCAATTGAAGAACCCT+TGG | - | 50606:16203-16222 | MS.gene050803:intergenic | 40.0% | |
| ACTCTCACCAAGAACTTGAT+TGG | - | 50606:16734-16753 | MS.gene050803:intergenic | 40.0% | |
| CAAAGGTTTCAAATCAACCC+TGG | - | 50606:16632-16651 | MS.gene050803:intergenic | 40.0% | |
| CAATTGAAGAACCCTTGGAA+AGG | - | 50606:16198-16217 | MS.gene050803:intergenic | 40.0% | |
| CATACATTGTCATCAGTCAG+TGG | + | 50606:16600-16619 | MS.gene050803:CDS | 40.0% | |
| GAGTCCATCTCATACTCTTA+AGG | + | 50606:15859-15878 | MS.gene050803:CDS | 40.0% | |
| GATACATCTTCTTTGCAAGC+AGG | - | 50606:16694-16713 | MS.gene050803:intergenic | 40.0% | |
| GCGAAAAAATCTGGACTATG+AGG | + | 50606:16870-16889 | MS.gene050803:CDS | 40.0% | |
| GGTGAAATTCATTACGGTTG+AGG | - | 50606:16410-16429 | MS.gene050803:intergenic | 40.0% | |
| TCATTACGGTTGAGGAAGAT+GGG | - | 50606:16402-16421 | MS.gene050803:intergenic | 40.0% | |
| TCCATTGCACAATTCAACCA+AGG | - | 50606:16025-16044 | MS.gene050803:intergenic | 40.0% | |
| TGACATTGATGCAACAACGA+CGG | - | 50606:16277-16296 | MS.gene050803:intergenic | 40.0% | |
| TTCATTACGGTTGAGGAAGA+TGG | - | 50606:16403-16422 | MS.gene050803:intergenic | 40.0% | |
| ! | CACTGACTGATGACAATGTA+TGG | - | 50606:16602-16621 | MS.gene050803:intergenic | 40.0% |
| ! | TGACCCTTAAGAGTATGAGA+TGG | - | 50606:15866-15885 | MS.gene050803:intergenic | 40.0% |
| !! | ATTTGATGACGATGATGCCT+TGG | + | 50606:16005-16024 | MS.gene050803:CDS | 40.0% |
| !! | CAGGGTTGATTTGAAACCTT+TGG | + | 50606:16630-16649 | MS.gene050803:CDS | 40.0% |
| !!! | TGGACTCGTTTTAGCTCTAT+TGG | - | 50606:15846-15865 | MS.gene050803:intergenic | 40.0% |
| ATGTGGCATAACTCATGCAG+AGG | - | 50606:15576-15595 | MS.gene050803:intergenic | 45.0% | |
| GCCTTGGTTGAATTGTGCAA+TGG | + | 50606:16021-16040 | MS.gene050803:CDS | 45.0% | |
| GGCAGCAAAGTCATTTGTAG+TGG | - | 50606:16101-16120 | MS.gene050803:intergenic | 45.0% | |
| GGTCTGCATTGAATCACACA+AGG | - | 50606:15755-15774 | MS.gene050803:intergenic | 45.0% | |
| GGTTTCAAATCAACCCTGGA+TGG | - | 50606:16628-16647 | MS.gene050803:intergenic | 45.0% | |
| TCAAGAGATTTCGGCTCCAA+AGG | - | 50606:16649-16668 | MS.gene050803:intergenic | 45.0% | |
| TCATCAAGACCAGAACGTGT+GGG | + | 50606:16487-16506 | MS.gene050803:CDS | 45.0% | |
| TCCTCTGTTTGCTCTGACAA+TGG | + | 50606:16808-16827 | MS.gene050803:CDS | 45.0% | |
| TGGACTATGAGGATTCTGAG+TGG | + | 50606:16881-16900 | MS.gene050803:CDS | 45.0% | |
| TTCATCAAGACCAGAACGTG+TGG | + | 50606:16486-16505 | MS.gene050803:CDS | 45.0% | |
| TTTCTTCACAAGGTGCATGC+TGG | - | 50606:16369-16388 | MS.gene050803:intergenic | 45.0% | |
| ! | AGCCGCAGGTTTTGCAAAAT+GGG | - | 50606:16431-16450 | MS.gene050803:intergenic | 45.0% |
| ! | CACCCATTTTGCAAAACCTG+CGG | + | 50606:16426-16445 | MS.gene050803:CDS | 45.0% |
| ! | TAGCCGCAGGTTTTGCAAAA+TGG | - | 50606:16432-16451 | MS.gene050803:intergenic | 45.0% |
| ! | TTAGCTTTCACAATAGCCGC+AGG | - | 50606:16445-16464 | MS.gene050803:intergenic | 45.0% |
| CTTGGTGAGAGTCGCAGAAA+AGG | + | 50606:16742-16761 | MS.gene050803:CDS | 50.0% | |
| GCCATTGTCAGAGCAAACAG+AGG | - | 50606:16812-16831 | MS.gene050803:intergenic | 50.0% | |
| TTCTTTGCTCCCACACGTTC+TGG | - | 50606:16499-16518 | MS.gene050803:intergenic | 50.0% | |
| ! | GATCATGGACGAGGCGTTAT+GGG | + | 50606:17051-17070 | MS.gene050803:CDS | 50.0% |
| ! | TGATCATGGACGAGGCGTTA+TGG | + | 50606:17050-17069 | MS.gene050803:CDS | 50.0% |
| !!! | GGTGCTAGTTTTTAGTCCCG+AGG | - | 50606:16239-16258 | MS.gene050803:intergenic | 50.0% |
| ATCAGTCAGTGGAGCCATCC+AGG | + | 50606:16611-16630 | MS.gene050803:CDS | 55.0% | |
| TCAGTCAGTGGAGCCATCCA+GGG | + | 50606:16612-16631 | MS.gene050803:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50606 | gene | 15567 | 17119 | 15567 | ID=MS.gene050803 |
| 50606 | mRNA | 15567 | 17119 | 15567 | ID=MS.gene050803.t1;Parent=MS.gene050803 |
| 50606 | exon | 15567 | 15658 | 15567 | ID=MS.gene050803.t1.exon1;Parent=MS.gene050803.t1 |
| 50606 | CDS | 15567 | 15658 | 15567 | ID=cds.MS.gene050803.t1;Parent=MS.gene050803.t1 |
| 50606 | exon | 15772 | 16912 | 15772 | ID=MS.gene050803.t1.exon2;Parent=MS.gene050803.t1 |
| 50606 | CDS | 15772 | 16912 | 15772 | ID=cds.MS.gene050803.t1;Parent=MS.gene050803.t1 |
| 50606 | exon | 17015 | 17119 | 17015 | ID=MS.gene050803.t1.exon3;Parent=MS.gene050803.t1 |
| 50606 | CDS | 17015 | 17119 | 17015 | ID=cds.MS.gene050803.t1;Parent=MS.gene050803.t1 |
| Gene Sequence |
| Protein sequence |
