Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051080.t1 | AFK46039.1 | 92.5 | 146 | 7 | 1 | 1 | 142 | 1 | 146 | 6.90E-06 | 60.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051080.t1 | G7L8A6 | 92.5 | 146 | 7 | 1 | 1 | 142 | 1 | 146 | 4.9e-06 | 60.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051080 | MS.gene30964 | -0.820656 | 6.11E-53 | -1.69E-46 |
| MS.gene051080 | MS.gene44135 | 0.803721 | 3.05E-49 | -1.69E-46 |
| MS.gene051080 | MS.gene45498 | -0.81991 | 9.06E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051080.t1 | MTR_8g022020 | 84.659 | 176 | 19 | 3 | 1 | 170 | 1 | 174 | 2.01e-56 | 175 |
| MS.gene051080.t1 | MTR_5g011490 | 75.294 | 85 | 17 | 2 | 38 | 120 | 15 | 97 | 2.91e-20 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051080.t1 | AT4G24370 | 36.364 | 165 | 56 | 8 | 1 | 139 | 1 | 142 | 3.00e-12 | 61.6 |
Find 45 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCAGATCGAGATCAAATT+CGG | 0.357799 | 8.3:-69805445 | None:intergenic |
| TTAGGATTGGGATTGGGATT+GGG | 0.359755 | 8.3:-69805408 | None:intergenic |
| TCGGGATCAGGATTAGTGTT+AGG | 0.362624 | 8.3:-69805426 | None:intergenic |
| TTGGGAATTGTTGAGGTAGA+AGG | 0.374094 | 8.3:-69805390 | None:intergenic |
| GTTAGGATTGGGATTGGGAT+TGG | 0.384111 | 8.3:-69805409 | None:intergenic |
| GGCGGCGGCAGGATTGGATT+TGG | 0.384673 | 8.3:-69805547 | None:intergenic |
| AGATTCGGAAATTGATTGAA+TGG | 0.391935 | 8.3:+69805628 | MS.gene051080:CDS |
| TCAGGATTAGTGTTAGGATT+GGG | 0.396732 | 8.3:-69805420 | None:intergenic |
| GACAGTGGCGGCGGCAGGAT+TGG | 0.411627 | 8.3:-69805553 | None:intergenic |
| ATTAGTGTTAGGATTGGGAT+TGG | 0.412835 | 8.3:-69805415 | None:intergenic |
| AGGTGAAGGACGAGATAGAT+GGG | 0.418413 | 8.3:-69805370 | None:intergenic |
| GATTCGGAAATTGATTGAAT+GGG | 0.423037 | 8.3:+69805629 | MS.gene051080:CDS |
| CAGAAAGCAAAAGAAAGAAC+CGG | 0.426272 | 8.3:+69805287 | MS.gene051080:CDS |
| TCGAGATCAAATTCGGGATC+AGG | 0.440122 | 8.3:-69805438 | None:intergenic |
| ATTTGTTCAACTTCATCTTC+CGG | 0.442682 | 8.3:-69805306 | None:intergenic |
| TTAGTGTTAGGATTGGGATT+GGG | 0.460564 | 8.3:-69805414 | None:intergenic |
| TCCAGATCGAGATCAAATTC+GGG | 0.478855 | 8.3:-69805444 | None:intergenic |
| TCCCGAATTTGATCTCGATC+TGG | 0.483403 | 8.3:+69805443 | MS.gene051080:CDS |
| ATCAGGATTAGTGTTAGGAT+TGG | 0.485333 | 8.3:-69805421 | None:intergenic |
| GTAAAGACAGTGGCGGCGGC+AGG | 0.485493 | 8.3:-69805558 | None:intergenic |
| AAGGTGAAGGACGAGATAGA+TGG | 0.487598 | 8.3:-69805371 | None:intergenic |
| AAGATGAAGAGGTTCAGATT+CGG | 0.499865 | 8.3:+69805613 | MS.gene051080:CDS |
| TGAAGAGGAAGAAGATGAAG+AGG | 0.505054 | 8.3:+69805602 | MS.gene051080:CDS |
| ATCAAAATCTCAACAACCGT+TGG | 0.524381 | 8.3:+69805494 | MS.gene051080:CDS |
| ACAGACGGCGACGGAGGAGA+AGG | 0.542208 | 8.3:-69805675 | None:intergenic |
| GTCGTCAGATACATCGGAAG+AGG | 0.549618 | 8.3:-69805724 | None:intergenic |
| CGATGTAAAGACAGTGGCGG+CGG | 0.564832 | 8.3:-69805562 | None:intergenic |
| GATGGGAATTGAGAGAGAGG+AGG | 0.567647 | 8.3:-69805353 | None:intergenic |
| ATAGATGGGAATTGAGAGAG+AGG | 0.569615 | 8.3:-69805356 | None:intergenic |
| ATTGGGATTGGGAATTGTTG+AGG | 0.596942 | 8.3:-69805397 | None:intergenic |
| ATTGTTGAGGTAGAAGGTGA+AGG | 0.614493 | 8.3:-69805384 | None:intergenic |
| AACTCAGTATGAAAGTGAAG+AGG | 0.616466 | 8.3:+69805587 | MS.gene051080:CDS |
| ATCGTCGTCGTCAGATACAT+CGG | 0.621613 | 8.3:-69805730 | None:intergenic |
| GAGGAGGATTTCGTCTTGTG+CGG | 0.625478 | 8.3:-69805337 | None:intergenic |
| TCGTCAGATACATCGGAAGA+GGG | 0.627438 | 8.3:-69805723 | None:intergenic |
| AGGATCGTGATCTTCATCAT+CGG | 0.635603 | 8.3:-69805700 | None:intergenic |
| CGCCGCCACTGTCTTTACAT+CGG | 0.646346 | 8.3:+69805563 | MS.gene051080:CDS |
| TCGGATACAGACGGCGACGG+AGG | 0.652745 | 8.3:-69805681 | None:intergenic |
| TCATCGGATACAGACGGCGA+CGG | 0.666925 | 8.3:-69805684 | None:intergenic |
| AATCTGGCGTTGATATCCAA+CGG | 0.669753 | 8.3:-69805510 | None:intergenic |
| TCAGATACATCGGAAGAGGG+AGG | 0.674537 | 8.3:-69805720 | None:intergenic |
| GCATCTGTGAAAATGAGTCG+AGG | 0.689675 | 8.3:+69805261 | None:intergenic |
| TCTTCATCATCGGATACAGA+CGG | 0.706044 | 8.3:-69805690 | None:intergenic |
| TTCCGATGTAAAGACAGTGG+CGG | 0.709410 | 8.3:-69805565 | None:intergenic |
| AGTTTCCGATGTAAAGACAG+TGG | 0.712727 | 8.3:-69805568 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATTCGGAAATTGATTGAA+TGG | + | chr8.3:69805628-69805647 | MS.gene051080:CDS | 30.0% | |
| ATTTGTTCAACTTCATCTTC+CGG | - | chr8.3:69805309-69805328 | None:intergenic | 30.0% | |
| GATTCGGAAATTGATTGAAT+GGG | + | chr8.3:69805629-69805648 | MS.gene051080:CDS | 30.0% | |
| AACTCAGTATGAAAGTGAAG+AGG | + | chr8.3:69805587-69805606 | MS.gene051080:CDS | 35.0% | |
| AAGATGAAGAGGTTCAGATT+CGG | + | chr8.3:69805613-69805632 | MS.gene051080:CDS | 35.0% | |
| ATCAAAATCTCAACAACCGT+TGG | + | chr8.3:69805494-69805513 | MS.gene051080:CDS | 35.0% | |
| ATTAGTGTTAGGATTGGGAT+TGG | - | chr8.3:69805418-69805437 | None:intergenic | 35.0% | |
| CAGAAAGCAAAAGAAAGAAC+CGG | + | chr8.3:69805287-69805306 | MS.gene051080:CDS | 35.0% | |
| TTAGTGTTAGGATTGGGATT+GGG | - | chr8.3:69805417-69805436 | None:intergenic | 35.0% | |
| TTCCAGATCGAGATCAAATT+CGG | - | chr8.3:69805448-69805467 | None:intergenic | 35.0% | |
| ! | ATCAGGATTAGTGTTAGGAT+TGG | - | chr8.3:69805424-69805443 | None:intergenic | 35.0% |
| ! | TCAGGATTAGTGTTAGGATT+GGG | - | chr8.3:69805423-69805442 | None:intergenic | 35.0% |
| AGGATCGTGATCTTCATCAT+CGG | - | chr8.3:69805703-69805722 | None:intergenic | 40.0% | |
| AGTTTCCGATGTAAAGACAG+TGG | - | chr8.3:69805571-69805590 | None:intergenic | 40.0% | |
| ATAGATGGGAATTGAGAGAG+AGG | - | chr8.3:69805359-69805378 | None:intergenic | 40.0% | |
| ATTGGGATTGGGAATTGTTG+AGG | - | chr8.3:69805400-69805419 | None:intergenic | 40.0% | |
| ATTGTTGAGGTAGAAGGTGA+AGG | - | chr8.3:69805387-69805406 | None:intergenic | 40.0% | |
| TCCAGATCGAGATCAAATTC+GGG | - | chr8.3:69805447-69805466 | None:intergenic | 40.0% | |
| TCTTCATCATCGGATACAGA+CGG | - | chr8.3:69805693-69805712 | None:intergenic | 40.0% | |
| TGAAGAGGAAGAAGATGAAG+AGG | + | chr8.3:69805602-69805621 | MS.gene051080:CDS | 40.0% | |
| TTAGGATTGGGATTGGGATT+GGG | - | chr8.3:69805411-69805430 | None:intergenic | 40.0% | |
| TTGGGAATTGTTGAGGTAGA+AGG | - | chr8.3:69805393-69805412 | None:intergenic | 40.0% | |
| !! | AATCTGGCGTTGATATCCAA+CGG | - | chr8.3:69805513-69805532 | None:intergenic | 40.0% |
| !!! | ATTTTTTGACTGGTTTGCGG+CGG | - | chr8.3:69805764-69805783 | None:intergenic | 40.0% |
| !!! | GGTTTTGAGAGAATCGAATC+TGG | - | chr8.3:69805529-69805548 | None:intergenic | 40.0% |
| AAGGTGAAGGACGAGATAGA+TGG | - | chr8.3:69805374-69805393 | None:intergenic | 45.0% | |
| AGGTGAAGGACGAGATAGAT+GGG | - | chr8.3:69805373-69805392 | None:intergenic | 45.0% | |
| ATCGTCGTCGTCAGATACAT+CGG | - | chr8.3:69805733-69805752 | None:intergenic | 45.0% | |
| GTTAGGATTGGGATTGGGAT+TGG | - | chr8.3:69805412-69805431 | None:intergenic | 45.0% | |
| TCCCGAATTTGATCTCGATC+TGG | + | chr8.3:69805443-69805462 | MS.gene051080:CDS | 45.0% | |
| TCGAGATCAAATTCGGGATC+AGG | - | chr8.3:69805441-69805460 | None:intergenic | 45.0% | |
| TCGTCAGATACATCGGAAGA+GGG | - | chr8.3:69805726-69805745 | None:intergenic | 45.0% | |
| TTCCGATGTAAAGACAGTGG+CGG | - | chr8.3:69805568-69805587 | None:intergenic | 45.0% | |
| ! | TCGGGATCAGGATTAGTGTT+AGG | - | chr8.3:69805429-69805448 | None:intergenic | 45.0% |
| GAGGAGGATTTCGTCTTGTG+CGG | - | chr8.3:69805340-69805359 | None:intergenic | 50.0% | |
| GATGGGAATTGAGAGAGAGG+AGG | - | chr8.3:69805356-69805375 | None:intergenic | 50.0% | |
| GTCGTCAGATACATCGGAAG+AGG | - | chr8.3:69805727-69805746 | None:intergenic | 50.0% | |
| TCAGATACATCGGAAGAGGG+AGG | - | chr8.3:69805723-69805742 | None:intergenic | 50.0% | |
| CGATGTAAAGACAGTGGCGG+CGG | - | chr8.3:69805565-69805584 | None:intergenic | 55.0% | |
| CGCCGCCACTGTCTTTACAT+CGG | + | chr8.3:69805563-69805582 | MS.gene051080:CDS | 55.0% | |
| TCATCGGATACAGACGGCGA+CGG | - | chr8.3:69805687-69805706 | None:intergenic | 55.0% | |
| ACAGACGGCGACGGAGGAGA+AGG | - | chr8.3:69805678-69805697 | None:intergenic | 65.0% | |
| GGCGGCGGCAGGATTGGATT+TGG | - | chr8.3:69805550-69805569 | None:intergenic | 65.0% | |
| GTAAAGACAGTGGCGGCGGC+AGG | - | chr8.3:69805561-69805580 | None:intergenic | 65.0% | |
| TCGGATACAGACGGCGACGG+AGG | - | chr8.3:69805684-69805703 | None:intergenic | 65.0% | |
| GACAGTGGCGGCGGCAGGAT+TGG | - | chr8.3:69805556-69805575 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 69805273 | 69805785 | 69805273 | ID=MS.gene051080 |
| chr8.3 | mRNA | 69805273 | 69805785 | 69805273 | ID=MS.gene051080.t1;Parent=MS.gene051080 |
| chr8.3 | exon | 69805273 | 69805785 | 69805273 | ID=MS.gene051080.t1.exon1;Parent=MS.gene051080.t1 |
| chr8.3 | CDS | 69805273 | 69805785 | 69805273 | ID=cds.MS.gene051080.t1;Parent=MS.gene051080.t1 |
| Gene Sequence |
| Protein sequence |
