AlfalfaGEDB | MS.gene051097

M. sativa cultivar XinJiangDaYe / MS.gene051097

KEGG: -
GO: -
PFAM: PF07911.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051097.t1	XP_003627329.2	99.4	156	1	0	1	156	1	156	3.00E-80	307.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051097.t1	G7L7L9	99.4	156	1	0	1	156	1	156	2.1e-80	307.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051097	MS.gene074439	0.922268	1.23E-88	-1.69E-46
MS.gene051097	MS.gene25103	0.949205	2.04E-107	-1.69E-46
MS.gene051097	MS.gene28530	0.816695	4.84E-52	-1.69E-46
MS.gene051097	MS.gene30398	0.803613	3.21E-49	-1.69E-46
MS.gene051097	MS.gene30423	0.928776	1.79E-92	-1.69E-46
MS.gene051097	MS.gene35858	0.807459	5.00E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene074439	MS.gene051097	PPI
MS.gene051097	MS.gene30423	PPI
MS.gene25103	MS.gene051097	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051097.t1	MTR_8g021190	99.359	156	1	0	1	156	1	156	9.65e-113	316
MS.gene051097.t1	MTR_3g105340	51.613	124	49	4	30	152	11	124	3.65e-37	124
MS.gene051097.t1	MTR_4g125030	53.509	114	52	1	10	122	1	114	1.17e-36	122
MS.gene051097.t1	MTR_4g125010	52.991	117	51	3	10	122	1	117	9.46e-35	119
MS.gene051097.t1	MTR_8g013990	46.970	132	56	5	29	154	61	184	4.34e-32	113
MS.gene051097.t1	MTR_5g006430	50.000	104	44	2	30	133	2	97	2.39e-31	108
MS.gene051097.t1	MTR_1g014930	45.600	125	55	5	34	153	20	136	2.10e-29	105
MS.gene051097.t1	MTR_2g101140	42.969	128	69	3	29	154	61	186	5.67e-26	97.8
MS.gene051097.t1	MTR_1g103550	45.556	90	43	2	30	118	2	86	7.32e-26	95.5
MS.gene051097.t1	MTR_3g109860	43.519	108	59	2	30	136	36	142	2.94e-25	94.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051097.t1	AT1G72510	53.939	165	65	4	1	154	1	165	8.54e-57	175
MS.gene051097.t1	AT1G72510	53.939	165	65	4	1	154	1	165	8.54e-57	175
MS.gene051097.t1	AT2G09970	48.193	166	74	5	1	154	1	166	4.18e-47	150
MS.gene051097.t1	AT5G20670	44.079	152	64	5	16	155	11	153	3.95e-31	109
MS.gene051097.t1	AT3G22540	44.860	107	54	2	30	136	2	103	1.89e-28	101
MS.gene051097.t1	AT4G14819	50.495	101	40	3	30	130	2	92	2.26e-28	101
MS.gene051097.t1	AT1G54095	41.727	139	67	4	29	156	14	149	7.40e-28	101
MS.gene051097.t1	AT5G25840	41.135	141	70	5	19	154	31	163	2.88e-24	92.8
MS.gene051097.t1	AT1G79770	44.828	116	58	3	6	121	31	140	2.13e-23	90.5
MS.gene051097.t1	AT2G25780	36.207	116	63	4	36	149	41	147	1.16e-18	77.8

Find 27 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTGCTTCTCTACTCTTTC+TGG	0.087534	8.3:+70058854	MS.gene051097:CDS
GAGAAAGGTATCAGGGAAAA+TGG	0.208570	8.3:+70058573	MS.gene051097:CDS
TCTCTAGTAGTCTTATTAAT+TGG	0.222630	8.3:-70058800	None:intergenic
CTACTCTTTCTGGTTCTTGA+TGG	0.251674	8.3:+70058864	MS.gene051097:CDS
CAGCTATGAGACAGATTCTT+AGG	0.252337	8.3:+70058747	MS.gene051097:CDS
AAGAATCAGAGAAAGGTATC+AGG	0.301816	8.3:+70058565	MS.gene051097:CDS
ATCAAATGAACTGCTGGATT+TGG	0.317985	8.3:-70058722	None:intergenic
TCTTGTGTTGATATAATCAT+TGG	0.343229	8.3:-70058437	None:intergenic
ACAGATTCTTAGGAGGAGTT+TGG	0.368015	8.3:+70058757	MS.gene051097:CDS
CTGATTCTTTCTATGTATGC+TGG	0.380458	8.3:-70058551	None:intergenic
ACTAGAGAAATTCATGGTTC+TGG	0.385021	8.3:+70058815	MS.gene051097:CDS
GTCTTATTAATTGGACTACT+TGG	0.397180	8.3:-70058791	None:intergenic
GCTGAAATCAAATGAACTGC+TGG	0.441735	8.3:-70058728	None:intergenic
AAGACTACTAGAGAAATTCA+TGG	0.460424	8.3:+70058809	MS.gene051097:CDS
AAATGAACTGCTGGATTTGG+AGG	0.460663	8.3:-70058719	None:intergenic
AGAAAGGTATCAGGGAAAAT+GGG	0.460947	8.3:+70058574	MS.gene051097:CDS
TTCTTAGGAGGAGTTTGGTT+AGG	0.460993	8.3:+70058762	MS.gene051097:CDS
TATCAGGGAAAATGGGTTTG+TGG	0.499942	8.3:+70058581	MS.gene051097:CDS
AAATGGGTTTGTGGATTATG+TGG	0.518267	8.3:+70058590	MS.gene051097:CDS
CTGTTAAAGATGAAATTGTA+AGG	0.538879	8.3:+70058618	MS.gene051097:CDS
AGAATCAGAGAAAGGTATCA+GGG	0.545546	8.3:+70058566	MS.gene051097:CDS
ATTAATTGGACTACTTGGTG+TGG	0.561690	8.3:-70058786	None:intergenic
ACATAGAAAGAATCAGAGAA+AGG	0.563326	8.3:+70058558	MS.gene051097:CDS
TGAACTGCTGGATTTGGAGG+AGG	0.575747	8.3:-70058716	None:intergenic
CTATGAGACAGATTCTTAGG+AGG	0.602915	8.3:+70058750	MS.gene051097:CDS
TTCATGGTTCTGGACTTGCT+AGG	0.647756	8.3:+70058825	MS.gene051097:CDS
TTTGCAATATGTGAATGCTG+TGG	0.702632	8.3:+70058509	MS.gene051097:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTGTTAAAGATGAAATTGTA+AGG	+	chr8.3:70058618-70058637	MS.gene051097:CDS	25.0%
!	TGCAAAAAATTCAATACTTC+AGG	+	chr8.3:70058692-70058711	MS.gene051097:CDS	25.0%
!!	TCTTGTGTTGATATAATCAT+TGG	-	chr8.3:70058440-70058459	None:intergenic	25.0%
!!!	TCTCTAGTAGTCTTATTAAT+TGG	-	chr8.3:70058803-70058822	None:intergenic	25.0%
	AAGACTACTAGAGAAATTCA+TGG	+	chr8.3:70058809-70058828	MS.gene051097:CDS	30.0%
	ACATAGAAAGAATCAGAGAA+AGG	+	chr8.3:70058558-70058577	MS.gene051097:CDS	30.0%
!	GTCTTATTAATTGGACTACT+TGG	-	chr8.3:70058794-70058813	None:intergenic	30.0%
	ACTAGAGAAATTCATGGTTC+TGG	+	chr8.3:70058815-70058834	MS.gene051097:CDS	35.0%
	ATCAAATGAACTGCTGGATT+TGG	-	chr8.3:70058725-70058744	None:intergenic	35.0%
	CTGATTCTTTCTATGTATGC+TGG	-	chr8.3:70058554-70058573	None:intergenic	35.0%
	TTTGCAATATGTGAATGCTG+TGG	+	chr8.3:70058509-70058528	MS.gene051097:CDS	35.0%
!	AAATGGGTTTGTGGATTATG+TGG	+	chr8.3:70058590-70058609	MS.gene051097:CDS	35.0%
!	ATTAATTGGACTACTTGGTG+TGG	-	chr8.3:70058789-70058808	None:intergenic	35.0%
!!	AAGAATCAGAGAAAGGTATC+AGG	+	chr8.3:70058565-70058584	MS.gene051097:CDS	35.0%
!!	AGAAAGGTATCAGGGAAAAT+GGG	+	chr8.3:70058574-70058593	MS.gene051097:CDS	35.0%
!!	AGAATCAGAGAAAGGTATCA+GGG	+	chr8.3:70058566-70058585	MS.gene051097:CDS	35.0%
	AAATGAACTGCTGGATTTGG+AGG	-	chr8.3:70058722-70058741	None:intergenic	40.0%
	ACAGATTCTTAGGAGGAGTT+TGG	+	chr8.3:70058757-70058776	MS.gene051097:CDS	40.0%
	CAGCTATGAGACAGATTCTT+AGG	+	chr8.3:70058747-70058766	MS.gene051097:CDS	40.0%
	CTATGAGACAGATTCTTAGG+AGG	+	chr8.3:70058750-70058769	MS.gene051097:CDS	40.0%
	GCTGAAATCAAATGAACTGC+TGG	-	chr8.3:70058731-70058750	None:intergenic	40.0%
	TTCTTAGGAGGAGTTTGGTT+AGG	+	chr8.3:70058762-70058781	MS.gene051097:CDS	40.0%
!	TATCAGGGAAAATGGGTTTG+TGG	+	chr8.3:70058581-70058600	MS.gene051097:CDS	40.0%
!!	GAGAAAGGTATCAGGGAAAA+TGG	+	chr8.3:70058573-70058592	MS.gene051097:CDS	40.0%
	AGCTGCTTCTCTACTCTTTC+TGG	+	chr8.3:70058854-70058873	MS.gene051097:CDS	45.0%
!!	TTCATGGTTCTGGACTTGCT+AGG	+	chr8.3:70058825-70058844	MS.gene051097:CDS	45.0%
	TGAACTGCTGGATTTGGAGG+AGG	-	chr8.3:70058719-70058738	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	70058413	70058883	70058413	ID=MS.gene051097
chr8.3	mRNA	70058413	70058883	70058413	ID=MS.gene051097.t1;Parent=MS.gene051097
chr8.3	exon	70058413	70058883	70058413	ID=MS.gene051097.t1.exon1;Parent=MS.gene051097.t1
chr8.3	CDS	70058413	70058883	70058413	ID=cds.MS.gene051097.t1;Parent=MS.gene051097.t1

Gene Sequence

>MS.gene051097

ATGTCTGCAACAATGATAAGTGATCCAATGATTATATCAACACAAGAAACTCAATCAACAACTAGTAGCATACTAGTTGCACAGAGTGAAGTTGAATTTGCAATATGTGAATGCTGTGGACTAACAGAAGAGTGCACACCAGCATACATAGAAAGAATCAGAGAAAGGTATCAGGGAAAATGGGTTTGTGGATTATGTGGTGAAGCTGTTAAAGATGAAATTGTAAGGTCAGAAAGACTTGTAAGCACAGAAGAAGCAATGACAAAACATATGAACTTTTGCAAAAAATTCAATACTTCAGGTCCTCCTCCAAATCCAGCAGTTCATTTGATTTCAGCTATGAGACAGATTCTTAGGAGGAGTTTGGTTAGGTCCACACCAAGTAGTCCAATTAATAAGACTACTAGAGAAATTCATGGTTCTGGACTTGCTAGGTCTGAAAGCTGCTTCTCTACTCTTTCTGGTTCTTGA

Protein sequence

>MS.gene051097.t1

MSATMISDPMIISTQETQSTTSSILVAQSEVEFAICECCGLTEECTPAYIERIRERYQGKWVCGLCGEAVKDEIVRSERLVSTEEAMTKHMNFCKKFNTSGPPPNPAVHLISAMRQILRRSLVRSTPSSPINKTTREIHGSGLARSESCFSTLSGS