Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30423.t1 | XP_003627329.2 | 99.4 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 3.00E-80 | 307.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30423.t1 | G7L7L9 | 99.4 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 2.1e-80 | 307.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene30423 | 0.805644 | 1.21E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene30423 | 0.805061 | 1.60E-49 | -1.69E-46 |
| MS.gene049807 | MS.gene30423 | 0.82798 | 1.16E-54 | -1.69E-46 |
| MS.gene051097 | MS.gene30423 | 0.928776 | 1.79E-92 | -1.69E-46 |
| MS.gene051852 | MS.gene30423 | 0.813037 | 3.13E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene30423 | 0.810443 | 1.15E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene30423 | 0.831653 | 1.48E-55 | -1.69E-46 |
| MS.gene052493 | MS.gene30423 | 0.809336 | 1.99E-50 | -1.69E-46 |
| MS.gene05350 | MS.gene30423 | 0.806937 | 6.45E-50 | -1.69E-46 |
| MS.gene05454 | MS.gene30423 | 0.801122 | 1.05E-48 | -1.69E-46 |
| MS.gene055141 | MS.gene30423 | 0.814575 | 1.44E-51 | -1.69E-46 |
| MS.gene055864 | MS.gene30423 | 0.807375 | 5.21E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene30423 | 0.831881 | 1.30E-55 | -1.69E-46 |
| MS.gene056699 | MS.gene30423 | 0.813525 | 2.45E-51 | -1.69E-46 |
| MS.gene058429 | MS.gene30423 | 0.813915 | 2.01E-51 | -1.69E-46 |
| MS.gene059774 | MS.gene30423 | 0.816515 | 5.32E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene30423 | 0.821473 | 3.96E-53 | -1.69E-46 |
| MS.gene060697 | MS.gene30423 | 0.816566 | 5.18E-52 | -1.69E-46 |
| MS.gene060785 | MS.gene30423 | 0.800905 | 1.16E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene30423 | 0.816557 | 5.20E-52 | -1.69E-46 |
| MS.gene061188 | MS.gene30423 | 0.813649 | 2.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051097 | MS.gene30423 | PPI |
| MS.gene074439 | MS.gene30423 | PPI |
| MS.gene25103 | MS.gene30423 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30423.t1 | MTR_8g021190 | 99.359 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 9.65e-113 | 316 |
| MS.gene30423.t1 | MTR_3g105340 | 51.613 | 124 | 49 | 4 | 30 | 152 | 11 | 124 | 3.65e-37 | 124 |
| MS.gene30423.t1 | MTR_4g125030 | 53.509 | 114 | 52 | 1 | 10 | 122 | 1 | 114 | 1.17e-36 | 122 |
| MS.gene30423.t1 | MTR_4g125010 | 52.991 | 117 | 51 | 3 | 10 | 122 | 1 | 117 | 9.46e-35 | 119 |
| MS.gene30423.t1 | MTR_8g013990 | 46.970 | 132 | 56 | 5 | 29 | 154 | 61 | 184 | 4.34e-32 | 113 |
| MS.gene30423.t1 | MTR_5g006430 | 50.000 | 104 | 44 | 2 | 30 | 133 | 2 | 97 | 2.39e-31 | 108 |
| MS.gene30423.t1 | MTR_1g014930 | 45.600 | 125 | 55 | 5 | 34 | 153 | 20 | 136 | 2.10e-29 | 105 |
| MS.gene30423.t1 | MTR_2g101140 | 42.969 | 128 | 69 | 3 | 29 | 154 | 61 | 186 | 5.67e-26 | 97.8 |
| MS.gene30423.t1 | MTR_1g103550 | 45.556 | 90 | 43 | 2 | 30 | 118 | 2 | 86 | 7.32e-26 | 95.5 |
| MS.gene30423.t1 | MTR_3g109860 | 43.519 | 108 | 59 | 2 | 30 | 136 | 36 | 142 | 2.94e-25 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30423.t1 | AT1G72510 | 53.939 | 165 | 65 | 4 | 1 | 154 | 1 | 165 | 8.54e-57 | 175 |
| MS.gene30423.t1 | AT1G72510 | 53.939 | 165 | 65 | 4 | 1 | 154 | 1 | 165 | 8.54e-57 | 175 |
| MS.gene30423.t1 | AT2G09970 | 48.193 | 166 | 74 | 5 | 1 | 154 | 1 | 166 | 4.18e-47 | 150 |
| MS.gene30423.t1 | AT5G20670 | 44.079 | 152 | 64 | 5 | 16 | 155 | 11 | 153 | 3.95e-31 | 109 |
| MS.gene30423.t1 | AT3G22540 | 44.860 | 107 | 54 | 2 | 30 | 136 | 2 | 103 | 1.89e-28 | 101 |
| MS.gene30423.t1 | AT4G14819 | 50.495 | 101 | 40 | 3 | 30 | 130 | 2 | 92 | 2.26e-28 | 101 |
| MS.gene30423.t1 | AT1G54095 | 41.727 | 139 | 67 | 4 | 29 | 156 | 14 | 149 | 7.40e-28 | 101 |
| MS.gene30423.t1 | AT5G25840 | 41.135 | 141 | 70 | 5 | 19 | 154 | 31 | 163 | 2.88e-24 | 92.8 |
| MS.gene30423.t1 | AT1G79770 | 44.828 | 116 | 58 | 3 | 6 | 121 | 31 | 140 | 2.13e-23 | 90.5 |
| MS.gene30423.t1 | AT2G25780 | 36.207 | 116 | 63 | 4 | 36 | 149 | 41 | 147 | 1.16e-18 | 77.8 |
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGCTTCTCTACTCTTTC+TGG | 0.087534 | 8.1:+78226999 | MS.gene30423:CDS |
| GAGAAAGGTATCAAGGAAAA+TGG | 0.211571 | 8.1:+78226718 | MS.gene30423:CDS |
| TCTCTAGTAGTCTTATTAAT+TGG | 0.222630 | 8.1:-78226945 | None:intergenic |
| CTACTCTTTCTGGTTCTTGA+TGG | 0.251674 | 8.1:+78227009 | MS.gene30423:CDS |
| CAGCTATGAGACAGATTCTT+AGG | 0.252337 | 8.1:+78226892 | MS.gene30423:CDS |
| ATCAAATGAACTGCTGGATT+TGG | 0.317985 | 8.1:-78226867 | None:intergenic |
| TCTTGTGTTGATATAATCAT+TGG | 0.343229 | 8.1:-78226582 | None:intergenic |
| ACAGATTCTTAGGAGGAGTT+TGG | 0.368015 | 8.1:+78226902 | MS.gene30423:CDS |
| CTGATTCTTTCTATGTATGC+TGG | 0.380458 | 8.1:-78226696 | None:intergenic |
| ACTAGAGAAATTCATGGTTC+TGG | 0.385021 | 8.1:+78226960 | MS.gene30423:CDS |
| GTCTTATTAATTGGACTACT+TGG | 0.397180 | 8.1:-78226936 | None:intergenic |
| GCTGAAATCAAATGAACTGC+TGG | 0.441735 | 8.1:-78226873 | None:intergenic |
| AAGACTACTAGAGAAATTCA+TGG | 0.460424 | 8.1:+78226954 | MS.gene30423:CDS |
| AAATGAACTGCTGGATTTGG+AGG | 0.460663 | 8.1:-78226864 | None:intergenic |
| TTCTTAGGAGGAGTTTGGTT+AGG | 0.460993 | 8.1:+78226907 | MS.gene30423:CDS |
| TATCAAGGAAAATGGGTTTG+TGG | 0.478219 | 8.1:+78226726 | MS.gene30423:CDS |
| AGAAAGGTATCAAGGAAAAT+GGG | 0.478410 | 8.1:+78226719 | MS.gene30423:CDS |
| AGAATCAGAGAAAGGTATCA+AGG | 0.494211 | 8.1:+78226711 | MS.gene30423:CDS |
| CTGTTAAAGATGAAATTGTA+AGG | 0.538879 | 8.1:+78226763 | MS.gene30423:CDS |
| ATTAATTGGACTACTTGGTG+TGG | 0.561690 | 8.1:-78226931 | None:intergenic |
| ACATAGAAAGAATCAGAGAA+AGG | 0.563326 | 8.1:+78226703 | MS.gene30423:CDS |
| AAATGGGTTTGTGGATTATG+TGG | 0.572200 | 8.1:+78226735 | MS.gene30423:CDS |
| TGAACTGCTGGATTTGGAGG+AGG | 0.575747 | 8.1:-78226861 | None:intergenic |
| CTATGAGACAGATTCTTAGG+AGG | 0.602915 | 8.1:+78226895 | MS.gene30423:CDS |
| TTCATGGTTCTGGACTTGCT+AGG | 0.647756 | 8.1:+78226970 | MS.gene30423:CDS |
| TTTGCAATATGTGAATGCTG+TGG | 0.702632 | 8.1:+78226654 | MS.gene30423:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTGTTAAAGATGAAATTGTA+AGG | + | chr8.1:78226763-78226782 | MS.gene30423:CDS | 25.0% |
| ! | TGCAAAAAATTCAATACTTC+AGG | + | chr8.1:78226837-78226856 | MS.gene30423:CDS | 25.0% |
| !! | TCTTGTGTTGATATAATCAT+TGG | - | chr8.1:78226585-78226604 | None:intergenic | 25.0% |
| !!! | TCTCTAGTAGTCTTATTAAT+TGG | - | chr8.1:78226948-78226967 | None:intergenic | 25.0% |
| AAGACTACTAGAGAAATTCA+TGG | + | chr8.1:78226954-78226973 | MS.gene30423:CDS | 30.0% | |
| ACATAGAAAGAATCAGAGAA+AGG | + | chr8.1:78226703-78226722 | MS.gene30423:CDS | 30.0% | |
| ! | GTCTTATTAATTGGACTACT+TGG | - | chr8.1:78226939-78226958 | None:intergenic | 30.0% |
| !! | AGAAAGGTATCAAGGAAAAT+GGG | + | chr8.1:78226719-78226738 | MS.gene30423:CDS | 30.0% |
| ACTAGAGAAATTCATGGTTC+TGG | + | chr8.1:78226960-78226979 | MS.gene30423:CDS | 35.0% | |
| ATCAAATGAACTGCTGGATT+TGG | - | chr8.1:78226870-78226889 | None:intergenic | 35.0% | |
| CTGATTCTTTCTATGTATGC+TGG | - | chr8.1:78226699-78226718 | None:intergenic | 35.0% | |
| TTTGCAATATGTGAATGCTG+TGG | + | chr8.1:78226654-78226673 | MS.gene30423:CDS | 35.0% | |
| ! | AAATGGGTTTGTGGATTATG+TGG | + | chr8.1:78226735-78226754 | MS.gene30423:CDS | 35.0% |
| ! | ATTAATTGGACTACTTGGTG+TGG | - | chr8.1:78226934-78226953 | None:intergenic | 35.0% |
| ! | TATCAAGGAAAATGGGTTTG+TGG | + | chr8.1:78226726-78226745 | MS.gene30423:CDS | 35.0% |
| !! | AGAATCAGAGAAAGGTATCA+AGG | + | chr8.1:78226711-78226730 | MS.gene30423:CDS | 35.0% |
| !! | GAGAAAGGTATCAAGGAAAA+TGG | + | chr8.1:78226718-78226737 | MS.gene30423:CDS | 35.0% |
| AAATGAACTGCTGGATTTGG+AGG | - | chr8.1:78226867-78226886 | None:intergenic | 40.0% | |
| ACAGATTCTTAGGAGGAGTT+TGG | + | chr8.1:78226902-78226921 | MS.gene30423:CDS | 40.0% | |
| CAGCTATGAGACAGATTCTT+AGG | + | chr8.1:78226892-78226911 | MS.gene30423:CDS | 40.0% | |
| CTATGAGACAGATTCTTAGG+AGG | + | chr8.1:78226895-78226914 | MS.gene30423:CDS | 40.0% | |
| GCTGAAATCAAATGAACTGC+TGG | - | chr8.1:78226876-78226895 | None:intergenic | 40.0% | |
| TTCTTAGGAGGAGTTTGGTT+AGG | + | chr8.1:78226907-78226926 | MS.gene30423:CDS | 40.0% | |
| AGCTGCTTCTCTACTCTTTC+TGG | + | chr8.1:78226999-78227018 | MS.gene30423:CDS | 45.0% | |
| !! | TTCATGGTTCTGGACTTGCT+AGG | + | chr8.1:78226970-78226989 | MS.gene30423:CDS | 45.0% |
| TGAACTGCTGGATTTGGAGG+AGG | - | chr8.1:78226864-78226883 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 78226558 | 78227028 | 78226558 | ID=MS.gene30423 |
| chr8.1 | mRNA | 78226558 | 78227028 | 78226558 | ID=MS.gene30423.t1;Parent=MS.gene30423 |
| chr8.1 | exon | 78226558 | 78227028 | 78226558 | ID=MS.gene30423.t1.exon1;Parent=MS.gene30423.t1 |
| chr8.1 | CDS | 78226558 | 78227028 | 78226558 | ID=cds.MS.gene30423.t1;Parent=MS.gene30423.t1 |
| Gene Sequence |
| Protein sequence |
