Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25103.t1 | XP_003627329.2 | 100 | 156 | 0 | 0 | 1 | 156 | 1 | 156 | 2.70E-81 | 311.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25103.t1 | G7L7L9 | 100.0 | 156 | 0 | 0 | 1 | 156 | 1 | 156 | 1.9e-81 | 311.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051097 | MS.gene25103 | 0.949205 | 2.04E-107 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene25103 | MS.gene051097 | PPI |
| MS.gene25103 | MS.gene30423 | PPI |
| MS.gene074439 | MS.gene25103 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25103.t1 | MTR_8g021190 | 100.000 | 156 | 0 | 0 | 1 | 156 | 1 | 156 | 6.34e-114 | 319 |
| MS.gene25103.t1 | MTR_3g105340 | 51.613 | 124 | 49 | 4 | 30 | 152 | 11 | 124 | 4.45e-37 | 124 |
| MS.gene25103.t1 | MTR_4g125030 | 53.509 | 114 | 52 | 1 | 10 | 122 | 1 | 114 | 1.41e-36 | 122 |
| MS.gene25103.t1 | MTR_4g125010 | 52.991 | 117 | 51 | 3 | 10 | 122 | 1 | 117 | 8.49e-35 | 119 |
| MS.gene25103.t1 | MTR_8g013990 | 46.970 | 132 | 56 | 5 | 29 | 154 | 61 | 184 | 4.78e-32 | 113 |
| MS.gene25103.t1 | MTR_5g006430 | 50.000 | 104 | 44 | 2 | 30 | 133 | 2 | 97 | 2.58e-31 | 108 |
| MS.gene25103.t1 | MTR_1g014930 | 45.600 | 125 | 55 | 5 | 34 | 153 | 20 | 136 | 1.95e-29 | 105 |
| MS.gene25103.t1 | MTR_2g101140 | 42.969 | 128 | 69 | 3 | 29 | 154 | 61 | 186 | 4.68e-26 | 98.2 |
| MS.gene25103.t1 | MTR_1g103550 | 45.556 | 90 | 43 | 2 | 30 | 118 | 2 | 86 | 6.86e-26 | 95.5 |
| MS.gene25103.t1 | MTR_3g109860 | 43.519 | 108 | 59 | 2 | 30 | 136 | 36 | 142 | 2.78e-25 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25103.t1 | AT1G72510 | 54.545 | 165 | 64 | 4 | 1 | 154 | 1 | 165 | 6.54e-58 | 178 |
| MS.gene25103.t1 | AT1G72510 | 54.545 | 165 | 64 | 4 | 1 | 154 | 1 | 165 | 6.54e-58 | 178 |
| MS.gene25103.t1 | AT2G09970 | 48.795 | 166 | 73 | 5 | 1 | 154 | 1 | 166 | 1.82e-48 | 154 |
| MS.gene25103.t1 | AT5G20670 | 44.079 | 152 | 64 | 5 | 16 | 155 | 11 | 153 | 5.65e-31 | 109 |
| MS.gene25103.t1 | AT3G22540 | 44.860 | 107 | 54 | 2 | 30 | 136 | 2 | 103 | 1.79e-28 | 101 |
| MS.gene25103.t1 | AT4G14819 | 50.495 | 101 | 40 | 3 | 30 | 130 | 2 | 92 | 2.31e-28 | 101 |
| MS.gene25103.t1 | AT1G54095 | 41.727 | 139 | 67 | 4 | 29 | 156 | 14 | 149 | 9.29e-28 | 101 |
| MS.gene25103.t1 | AT5G25840 | 40.411 | 146 | 74 | 5 | 14 | 154 | 26 | 163 | 5.99e-25 | 94.7 |
| MS.gene25103.t1 | AT1G79770 | 52.174 | 92 | 42 | 2 | 30 | 121 | 51 | 140 | 2.98e-23 | 90.1 |
| MS.gene25103.t1 | AT2G25780 | 36.207 | 116 | 63 | 4 | 36 | 149 | 41 | 147 | 1.33e-18 | 77.8 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGTTGTTGATTGAGTTTC+TGG | 0.004073 | 8.4:-72517881 | None:intergenic |
| AGCTGCTTCTCTACTCTTTC+TGG | 0.087534 | 8.4:+72518280 | MS.gene25103:CDS |
| GAGAAAGGTATCAAGGAAAA+TGG | 0.211571 | 8.4:+72517999 | MS.gene25103:CDS |
| TCTCTAGTAGTCTTATTAAT+TGG | 0.222630 | 8.4:-72518226 | None:intergenic |
| CTACTCTTTCTGGTTCTTGA+TGG | 0.251674 | 8.4:+72518290 | MS.gene25103:CDS |
| CAGCTATGAGACAGATTCTT+AGG | 0.252337 | 8.4:+72518173 | MS.gene25103:CDS |
| ATCAAATGAACTGCTGGATT+TGG | 0.317985 | 8.4:-72518148 | None:intergenic |
| TCTGGTGTTGATATAATCAT+TGG | 0.339933 | 8.4:-72517863 | None:intergenic |
| ACAGATTCTTAGGAGGAGTT+TGG | 0.368015 | 8.4:+72518183 | MS.gene25103:CDS |
| CTGATTCTTTCTATGTATGC+TGG | 0.380458 | 8.4:-72517977 | None:intergenic |
| ACTAGAGAAATTCATGGTTC+TGG | 0.385021 | 8.4:+72518241 | MS.gene25103:CDS |
| GTCTTATTAATTGGACTACT+TGG | 0.397180 | 8.4:-72518217 | None:intergenic |
| GCTGAAATCAAATGAACTGC+TGG | 0.441735 | 8.4:-72518154 | None:intergenic |
| AAGACTACTAGAGAAATTCA+TGG | 0.460424 | 8.4:+72518235 | MS.gene25103:CDS |
| AAATGAACTGCTGGATTTGG+AGG | 0.460663 | 8.4:-72518145 | None:intergenic |
| TTCTTAGGAGGAGTTTGGTT+AGG | 0.460993 | 8.4:+72518188 | MS.gene25103:CDS |
| TATCAAGGAAAATGGGTTTG+TGG | 0.478219 | 8.4:+72518007 | MS.gene25103:CDS |
| AGAAAGGTATCAAGGAAAAT+GGG | 0.478410 | 8.4:+72518000 | MS.gene25103:CDS |
| AGAATCAGAGAAAGGTATCA+AGG | 0.494211 | 8.4:+72517992 | MS.gene25103:CDS |
| CTGTTAAAGATGAAATTGTA+AGG | 0.538879 | 8.4:+72518044 | MS.gene25103:CDS |
| ATTAATTGGACTACTTGGTG+TGG | 0.561690 | 8.4:-72518212 | None:intergenic |
| ACATAGAAAGAATCAGAGAA+AGG | 0.563326 | 8.4:+72517984 | MS.gene25103:CDS |
| AAATGGGTTTGTGGATTATG+TGG | 0.572200 | 8.4:+72518016 | MS.gene25103:CDS |
| TGAACTGCTGGATTTGGAGG+AGG | 0.575747 | 8.4:-72518142 | None:intergenic |
| CTATGAGACAGATTCTTAGG+AGG | 0.602915 | 8.4:+72518176 | MS.gene25103:CDS |
| TTCATGGTTCTGGACTTGCT+AGG | 0.647756 | 8.4:+72518251 | MS.gene25103:CDS |
| TTTGCAATATGTGAATGCTG+TGG | 0.702632 | 8.4:+72517935 | MS.gene25103:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTGTTAAAGATGAAATTGTA+AGG | + | chr8.4:72518044-72518063 | MS.gene25103:CDS | 25.0% |
| ! | TGCAAAAAATTCAATACTTC+AGG | + | chr8.4:72518118-72518137 | MS.gene25103:CDS | 25.0% |
| !!! | TCTCTAGTAGTCTTATTAAT+TGG | - | chr8.4:72518229-72518248 | None:intergenic | 25.0% |
| AAGACTACTAGAGAAATTCA+TGG | + | chr8.4:72518235-72518254 | MS.gene25103:CDS | 30.0% | |
| ACATAGAAAGAATCAGAGAA+AGG | + | chr8.4:72517984-72518003 | MS.gene25103:CDS | 30.0% | |
| ! | GTCTTATTAATTGGACTACT+TGG | - | chr8.4:72518220-72518239 | None:intergenic | 30.0% |
| !! | AGAAAGGTATCAAGGAAAAT+GGG | + | chr8.4:72518000-72518019 | MS.gene25103:CDS | 30.0% |
| !! | TCTGGTGTTGATATAATCAT+TGG | - | chr8.4:72517866-72517885 | None:intergenic | 30.0% |
| ACTAGAGAAATTCATGGTTC+TGG | + | chr8.4:72518241-72518260 | MS.gene25103:CDS | 35.0% | |
| ATCAAATGAACTGCTGGATT+TGG | - | chr8.4:72518151-72518170 | None:intergenic | 35.0% | |
| CTGATTCTTTCTATGTATGC+TGG | - | chr8.4:72517980-72517999 | None:intergenic | 35.0% | |
| TTTGCAATATGTGAATGCTG+TGG | + | chr8.4:72517935-72517954 | MS.gene25103:CDS | 35.0% | |
| ! | AAATGGGTTTGTGGATTATG+TGG | + | chr8.4:72518016-72518035 | MS.gene25103:CDS | 35.0% |
| ! | ATTAATTGGACTACTTGGTG+TGG | - | chr8.4:72518215-72518234 | None:intergenic | 35.0% |
| ! | CTAGTTGTTGATTGAGTTTC+TGG | - | chr8.4:72517884-72517903 | None:intergenic | 35.0% |
| ! | TATCAAGGAAAATGGGTTTG+TGG | + | chr8.4:72518007-72518026 | MS.gene25103:CDS | 35.0% |
| !! | AGAATCAGAGAAAGGTATCA+AGG | + | chr8.4:72517992-72518011 | MS.gene25103:CDS | 35.0% |
| !! | GAGAAAGGTATCAAGGAAAA+TGG | + | chr8.4:72517999-72518018 | MS.gene25103:CDS | 35.0% |
| AAATGAACTGCTGGATTTGG+AGG | - | chr8.4:72518148-72518167 | None:intergenic | 40.0% | |
| ACAGATTCTTAGGAGGAGTT+TGG | + | chr8.4:72518183-72518202 | MS.gene25103:CDS | 40.0% | |
| CAGCTATGAGACAGATTCTT+AGG | + | chr8.4:72518173-72518192 | MS.gene25103:CDS | 40.0% | |
| CTATGAGACAGATTCTTAGG+AGG | + | chr8.4:72518176-72518195 | MS.gene25103:CDS | 40.0% | |
| GCTGAAATCAAATGAACTGC+TGG | - | chr8.4:72518157-72518176 | None:intergenic | 40.0% | |
| TTCTTAGGAGGAGTTTGGTT+AGG | + | chr8.4:72518188-72518207 | MS.gene25103:CDS | 40.0% | |
| AGCTGCTTCTCTACTCTTTC+TGG | + | chr8.4:72518280-72518299 | MS.gene25103:CDS | 45.0% | |
| !! | TTCATGGTTCTGGACTTGCT+AGG | + | chr8.4:72518251-72518270 | MS.gene25103:CDS | 45.0% |
| TGAACTGCTGGATTTGGAGG+AGG | - | chr8.4:72518145-72518164 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 72517839 | 72518309 | 72517839 | ID=MS.gene25103 |
| chr8.4 | mRNA | 72517839 | 72518309 | 72517839 | ID=MS.gene25103.t1;Parent=MS.gene25103 |
| chr8.4 | exon | 72517839 | 72518309 | 72517839 | ID=MS.gene25103.t1.exon1;Parent=MS.gene25103.t1 |
| chr8.4 | CDS | 72517839 | 72518309 | 72517839 | ID=cds.MS.gene25103.t1;Parent=MS.gene25103.t1 |
| Gene Sequence |
| Protein sequence |
