AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074439

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074439.t1 MTR_8g021190 99.359 156 1 0 1 156 1 156 9.65e-113 316
MS.gene074439.t1 MTR_3g105340 51.613 124 49 4 30 152 11 124 3.65e-37 124
MS.gene074439.t1 MTR_4g125030 53.509 114 52 1 10 122 1 114 1.17e-36 122
MS.gene074439.t1 MTR_4g125010 52.991 117 51 3 10 122 1 117 9.46e-35 119
MS.gene074439.t1 MTR_8g013990 46.970 132 56 5 29 154 61 184 4.34e-32 113
MS.gene074439.t1 MTR_5g006430 50.000 104 44 2 30 133 2 97 2.39e-31 108
MS.gene074439.t1 MTR_1g014930 45.600 125 55 5 34 153 20 136 2.10e-29 105
MS.gene074439.t1 MTR_2g101140 42.969 128 69 3 29 154 61 186 5.67e-26 97.8
MS.gene074439.t1 MTR_1g103550 45.556 90 43 2 30 118 2 86 7.32e-26 95.5
MS.gene074439.t1 MTR_3g109860 43.519 108 59 2 30 136 36 142 2.94e-25 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074439.t1 AT1G72510 53.939 165 65 4 1 154 1 165 8.54e-57 175
MS.gene074439.t1 AT1G72510 53.939 165 65 4 1 154 1 165 8.54e-57 175
MS.gene074439.t1 AT2G09970 48.193 166 74 5 1 154 1 166 4.18e-47 150
MS.gene074439.t1 AT5G20670 44.079 152 64 5 16 155 11 153 3.95e-31 109
MS.gene074439.t1 AT3G22540 44.860 107 54 2 30 136 2 103 1.89e-28 101
MS.gene074439.t1 AT4G14819 50.495 101 40 3 30 130 2 92 2.26e-28 101
MS.gene074439.t1 AT1G54095 41.727 139 67 4 29 156 14 149 7.40e-28 101
MS.gene074439.t1 AT5G25840 41.135 141 70 5 19 154 31 163 2.88e-24 92.8
MS.gene074439.t1 AT1G79770 44.828 116 58 3 6 121 31 140 2.13e-23 90.5
MS.gene074439.t1 AT2G25780 36.207 116 63 4 36 149 41 147 1.16e-18 77.8

Find 27 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGCTTCTCTACTCTTTC+TGG 0.087534 8.2:+73851609 MS.gene074439:CDS
GAGAAAGGTATCAGGGAAAA+TGG 0.208570 8.2:+73851328 MS.gene074439:CDS
TCTCTAGTAGTCTTATTAAT+TGG 0.222630 8.2:-73851555 None:intergenic
CTACTCTTTCTGGTTCTTGA+TGG 0.251674 8.2:+73851619 MS.gene074439:CDS
CAGCTATGAGACAGATTCTT+AGG 0.252337 8.2:+73851502 MS.gene074439:CDS
AAGAATCAGAGAAAGGTATC+AGG 0.301816 8.2:+73851320 MS.gene074439:CDS
ATCAAATGAACTGCTGGATT+TGG 0.317985 8.2:-73851477 None:intergenic
TCTTGTGTTGATATAATCAT+TGG 0.343229 8.2:-73851192 None:intergenic
ACAGATTCTTAGGAGGAGTT+TGG 0.368015 8.2:+73851512 MS.gene074439:CDS
CTGATTCTTTCTATGTATGC+TGG 0.380458 8.2:-73851306 None:intergenic
ACTAGAGAAATTCATGGTTC+TGG 0.385021 8.2:+73851570 MS.gene074439:CDS
GTCTTATTAATTGGACTACT+TGG 0.397180 8.2:-73851546 None:intergenic
GCTGAAATCAAATGAACTGC+TGG 0.441735 8.2:-73851483 None:intergenic
AAGACTACTAGAGAAATTCA+TGG 0.460424 8.2:+73851564 MS.gene074439:CDS
AAATGAACTGCTGGATTTGG+AGG 0.460663 8.2:-73851474 None:intergenic
AGAAAGGTATCAGGGAAAAT+GGG 0.460947 8.2:+73851329 MS.gene074439:CDS
TTCTTAGGAGGAGTTTGGTT+AGG 0.460993 8.2:+73851517 MS.gene074439:CDS
TATCAGGGAAAATGGGTTTG+TGG 0.499942 8.2:+73851336 MS.gene074439:CDS
AAATGGGTTTGTGGATTATG+TGG 0.518267 8.2:+73851345 MS.gene074439:CDS
CTGTTAAAGATGAAATTGTA+AGG 0.538879 8.2:+73851373 MS.gene074439:CDS
AGAATCAGAGAAAGGTATCA+GGG 0.545546 8.2:+73851321 MS.gene074439:CDS
ATTAATTGGACTACTTGGTG+TGG 0.561690 8.2:-73851541 None:intergenic
ACATAGAAAGAATCAGAGAA+AGG 0.563326 8.2:+73851313 MS.gene074439:CDS
TGAACTGCTGGATTTGGAGG+AGG 0.575747 8.2:-73851471 None:intergenic
CTATGAGACAGATTCTTAGG+AGG 0.602915 8.2:+73851505 MS.gene074439:CDS
TTCATGGTTCTGGACTTGCT+AGG 0.647756 8.2:+73851580 MS.gene074439:CDS
TTTGCAATATGTGAATGCTG+TGG 0.702632 8.2:+73851264 MS.gene074439:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTGTTAAAGATGAAATTGTA+AGG + chr8.2:73851373-73851392 MS.gene074439:CDS 25.0%
! TGCAAAAAATTCAATACTTC+AGG + chr8.2:73851447-73851466 MS.gene074439:CDS 25.0%
!! TCTTGTGTTGATATAATCAT+TGG - chr8.2:73851195-73851214 None:intergenic 25.0%
!!! TCTCTAGTAGTCTTATTAAT+TGG - chr8.2:73851558-73851577 None:intergenic 25.0%
AAGACTACTAGAGAAATTCA+TGG + chr8.2:73851564-73851583 MS.gene074439:CDS 30.0%
ACATAGAAAGAATCAGAGAA+AGG + chr8.2:73851313-73851332 MS.gene074439:CDS 30.0%
! GTCTTATTAATTGGACTACT+TGG - chr8.2:73851549-73851568 None:intergenic 30.0%
ACTAGAGAAATTCATGGTTC+TGG + chr8.2:73851570-73851589 MS.gene074439:CDS 35.0%
ATCAAATGAACTGCTGGATT+TGG - chr8.2:73851480-73851499 None:intergenic 35.0%
CTGATTCTTTCTATGTATGC+TGG - chr8.2:73851309-73851328 None:intergenic 35.0%
TTTGCAATATGTGAATGCTG+TGG + chr8.2:73851264-73851283 MS.gene074439:CDS 35.0%
! AAATGGGTTTGTGGATTATG+TGG + chr8.2:73851345-73851364 MS.gene074439:CDS 35.0%
! ATTAATTGGACTACTTGGTG+TGG - chr8.2:73851544-73851563 None:intergenic 35.0%
!! AAGAATCAGAGAAAGGTATC+AGG + chr8.2:73851320-73851339 MS.gene074439:CDS 35.0%
!! AGAAAGGTATCAGGGAAAAT+GGG + chr8.2:73851329-73851348 MS.gene074439:CDS 35.0%
!! AGAATCAGAGAAAGGTATCA+GGG + chr8.2:73851321-73851340 MS.gene074439:CDS 35.0%
AAATGAACTGCTGGATTTGG+AGG - chr8.2:73851477-73851496 None:intergenic 40.0%
ACAGATTCTTAGGAGGAGTT+TGG + chr8.2:73851512-73851531 MS.gene074439:CDS 40.0%
CAGCTATGAGACAGATTCTT+AGG + chr8.2:73851502-73851521 MS.gene074439:CDS 40.0%
CTATGAGACAGATTCTTAGG+AGG + chr8.2:73851505-73851524 MS.gene074439:CDS 40.0%
GCTGAAATCAAATGAACTGC+TGG - chr8.2:73851486-73851505 None:intergenic 40.0%
TTCTTAGGAGGAGTTTGGTT+AGG + chr8.2:73851517-73851536 MS.gene074439:CDS 40.0%
! TATCAGGGAAAATGGGTTTG+TGG + chr8.2:73851336-73851355 MS.gene074439:CDS 40.0%
!! GAGAAAGGTATCAGGGAAAA+TGG + chr8.2:73851328-73851347 MS.gene074439:CDS 40.0%
AGCTGCTTCTCTACTCTTTC+TGG + chr8.2:73851609-73851628 MS.gene074439:CDS 45.0%
!! TTCATGGTTCTGGACTTGCT+AGG + chr8.2:73851580-73851599 MS.gene074439:CDS 45.0%
TGAACTGCTGGATTTGGAGG+AGG - chr8.2:73851474-73851493 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 73851168 73851638 73851168 ID=MS.gene074439
chr8.2 mRNA 73851168 73851638 73851168 ID=MS.gene074439.t1;Parent=MS.gene074439
chr8.2 exon 73851168 73851638 73851168 ID=MS.gene074439.t1.exon1;Parent=MS.gene074439.t1
chr8.2 CDS 73851168 73851638 73851168 ID=cds.MS.gene074439.t1;Parent=MS.gene074439.t1
Gene Sequence

>MS.gene074439

ATGTCTGCAACAATGATAAGTGATCCAATGATTATATCAACACAAGAAACTCAATCAACAACTAGTAGCATACTAGTTGCACAGAGTGAAGTTGAATTTGCAATATGTGAATGCTGTGGACTAACAGAAGAATGCACACCAGCATACATAGAAAGAATCAGAGAAAGGTATCAGGGAAAATGGGTTTGTGGATTATGTGGTGAAGCTGTTAAAGATGAAATTGTAAGGTCAGAAAGACTTGTAAGCACAGAAGAAGCAATGACAAAACATATGAACTTTTGCAAAAAATTCAATACTTCAGGTCCTCCTCCAAATCCAGCAGTTCATTTGATTTCAGCTATGAGACAGATTCTTAGGAGGAGTTTGGTTAGGTCCACACCAAGTAGTCCAATTAATAAGACTACTAGAGAAATTCATGGTTCTGGACTTGCTAGGTCTGAAAGCTGCTTCTCTACTCTTTCTGGTTCTTGA
Protein sequence

>MS.gene074439.t1

MSATMISDPMIISTQETQSTTSSILVAQSEVEFAICECCGLTEECTPAYIERIRERYQGKWVCGLCGEAVKDEIVRSERLVSTEEAMTKHMNFCKKFNTSGPPPNPAVHLISAMRQILRRSLVRSTPSSPINKTTREIHGSGLARSESCFSTLSGS