Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051622.t1 | XP_003589022.1 | 96.9 | 350 | 11 | 0 | 1 | 350 | 1 | 350 | 3.80E-192 | 680.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051622.t1 | Q944K3 | 77.8 | 338 | 75 | 0 | 6 | 343 | 47 | 384 | 8.0e-151 | 534.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051622.t1 | G7IB89 | 96.9 | 350 | 11 | 0 | 1 | 350 | 1 | 350 | 2.7e-192 | 680.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051622 | MS.gene24549 | 0.946546 | 3.75E-105 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051622 | MS.gene00617 | PPI |
| MS.gene051622 | MS.gene047200 | PPI |
| MS.gene051622 | MS.gene072058 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051622.t1 | MTR_1g016440 | 96.857 | 350 | 11 | 0 | 1 | 350 | 1 | 350 | 0.0 | 695 |
| MS.gene051622.t1 | MTR_1g016440 | 94.139 | 273 | 13 | 1 | 1 | 273 | 1 | 270 | 0.0 | 526 |
| MS.gene051622.t1 | MTR_4g109500 | 25.948 | 343 | 212 | 12 | 32 | 344 | 18 | 348 | 1.74e-20 | 93.2 |
| MS.gene051622.t1 | MTR_4g109500 | 25.948 | 343 | 212 | 12 | 32 | 344 | 18 | 348 | 2.00e-20 | 92.8 |
| MS.gene051622.t1 | MTR_5g004640 | 26.354 | 277 | 177 | 9 | 52 | 311 | 40 | 306 | 8.24e-16 | 78.6 |
| MS.gene051622.t1 | MTR_3g077160 | 24.843 | 318 | 190 | 10 | 52 | 344 | 45 | 338 | 8.58e-16 | 78.6 |
| MS.gene051622.t1 | MTR_4g055080 | 26.263 | 297 | 181 | 9 | 52 | 325 | 24 | 305 | 1.17e-15 | 78.2 |
| MS.gene051622.t1 | MTR_3g118535 | 26.897 | 290 | 192 | 8 | 52 | 327 | 37 | 320 | 2.54e-15 | 77.4 |
| MS.gene051622.t1 | MTR_4g055080 | 26.871 | 294 | 174 | 10 | 58 | 325 | 2 | 280 | 3.56e-15 | 76.6 |
| MS.gene051622.t1 | MTR_8g093800 | 25.587 | 383 | 226 | 16 | 3 | 335 | 10 | 383 | 9.46e-14 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051622.t1 | AT5G26040 | 77.419 | 341 | 77 | 0 | 3 | 343 | 44 | 384 | 0.0 | 548 |
| MS.gene051622.t1 | AT5G26040 | 71.006 | 338 | 70 | 1 | 6 | 343 | 47 | 356 | 7.56e-173 | 484 |
| MS.gene051622.t1 | AT5G63110 | 31.579 | 228 | 129 | 9 | 128 | 344 | 118 | 329 | 2.76e-18 | 86.3 |
| MS.gene051622.t1 | AT4G38130 | 26.374 | 273 | 182 | 6 | 52 | 311 | 36 | 302 | 4.49e-17 | 82.4 |
| MS.gene051622.t1 | AT4G38130 | 26.374 | 273 | 182 | 6 | 52 | 311 | 36 | 302 | 6.49e-17 | 82.0 |
| MS.gene051622.t1 | AT5G61060 | 26.452 | 310 | 203 | 8 | 32 | 320 | 26 | 331 | 1.87e-16 | 80.9 |
| MS.gene051622.t1 | AT1G08460 | 22.977 | 309 | 211 | 7 | 36 | 325 | 23 | 323 | 5.22e-15 | 75.9 |
| MS.gene051622.t1 | AT5G61060 | 26.433 | 314 | 202 | 9 | 32 | 320 | 26 | 335 | 5.43e-15 | 76.6 |
| MS.gene051622.t1 | AT5G61070 | 25.175 | 286 | 177 | 10 | 62 | 320 | 89 | 364 | 3.68e-13 | 70.9 |
| MS.gene051622.t1 | AT4G33470 | 25.806 | 186 | 129 | 3 | 159 | 335 | 201 | 386 | 2.39e-12 | 68.2 |
| MS.gene051622.t1 | AT1G08460 | 22.394 | 259 | 177 | 5 | 36 | 277 | 23 | 274 | 1.39e-11 | 65.1 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCGTTTGATTCATCTAAA+TGG | 0.178374 | 1.3:-5806839 | MS.gene051622:CDS |
| CATCACTGTTCTGCAGAAAA+TGG | 0.209362 | 1.3:-5805121 | MS.gene051622:CDS |
| TGTAAGTTTCTTAATTCTTT+TGG | 0.262598 | 1.3:-5806807 | MS.gene051622:CDS |
| AAAACCTCCTCCCACATTAA+TGG | 0.273424 | 1.3:+5805144 | None:intergenic |
| CCTATTGTCATGCTCACTTC+AGG | 0.309644 | 1.3:-5790576 | MS.gene051622:intron |
| GATCACTGTTATGCAGTCTT+TGG | 0.318259 | 1.3:+5789864 | None:intergenic |
| CTGATGTGTTTCAAGGTTGC+TGG | 0.321961 | 1.3:-5790935 | MS.gene051622:intron |
| TTCCTAATGGATCACCTTCT+AGG | 0.332310 | 1.3:+5790864 | None:intergenic |
| ATTACCTTCAACCTTCCTAA+TGG | 0.340809 | 1.3:+5790851 | None:intergenic |
| GGAATTGGTTGTTACTTTAG+AGG | 0.343735 | 1.3:+5807080 | None:intergenic |
| TCCGTTTGATTCATCTAAAT+GGG | 0.360447 | 1.3:-5806838 | MS.gene051622:CDS |
| TTTACCAAAGGATATATTCC+AGG | 0.381434 | 1.3:+5804762 | None:intergenic |
| TCTTATGACATATCATTTCT+CGG | 0.392913 | 1.3:-5806962 | MS.gene051622:CDS |
| AGAGTTTGGTAGAGAGGATA+CGG | 0.410858 | 1.3:+5807118 | None:intergenic |
| TCAAGGAAATGGTCATGAAA+TGG | 0.415584 | 1.3:-5804913 | MS.gene051622:CDS |
| GCAGGTTGGAGGAACTATAT+TGG | 0.416403 | 1.3:-5805197 | MS.gene051622:intron |
| CGAAAGTACCTCAATTATCT+TGG | 0.426478 | 1.3:+5805668 | None:intergenic |
| GATTTACCTGTTTCCTGAAT+GGG | 0.426483 | 1.3:+5805282 | None:intergenic |
| GGATTTACCTGTTTCCTGAA+TGG | 0.439631 | 1.3:+5805281 | None:intergenic |
| CTTGATGCACATCAAGGAAA+TGG | 0.443894 | 1.3:-5804924 | MS.gene051622:CDS |
| CCTGAAGTGAGCATGACAAT+AGG | 0.446956 | 1.3:+5790576 | None:intergenic |
| CTCAATTATCTTGGCAACAT+TGG | 0.449351 | 1.3:+5805677 | None:intergenic |
| TGTTCTATATTAGGATTATG+AGG | 0.464505 | 1.3:-5791510 | MS.gene051622:intron |
| AGGTACGTCGAAGTAGAGTT+TGG | 0.467538 | 1.3:+5807104 | None:intergenic |
| CATGAGAGTGTCGTCGGAAG+CGG | 0.470829 | 1.3:+5807146 | None:intergenic |
| TAACCCTGGAATATATCCTT+TGG | 0.472285 | 1.3:-5804766 | MS.gene051622:intron |
| GGCAGACCGAGTCTACATTC+TGG | 0.475079 | 1.3:-5804796 | MS.gene051622:intron |
| GAGTTGTTAGTTTATAATGC+TGG | 0.477061 | 1.3:-5790896 | MS.gene051622:CDS |
| TTTACGTTGCACAAGACCAT+TGG | 0.481724 | 1.3:+5805313 | None:intergenic |
| GAATTGGTTGTTACTTTAGA+GGG | 0.487635 | 1.3:+5807081 | None:intergenic |
| TTGTTCAGTTGAATATATCA+AGG | 0.488156 | 1.3:-5805048 | MS.gene051622:intron |
| TTACGTTGCACAAGACCATT+GGG | 0.493432 | 1.3:+5805314 | None:intergenic |
| AAATGATATGTCATAAGAAT+CGG | 0.499520 | 1.3:+5806967 | None:intergenic |
| GAATCAGAAAGTTGAAGTAA+AGG | 0.500918 | 1.3:-5791474 | MS.gene051622:intron |
| ATTGATCTTGATGCACATCA+AGG | 0.501233 | 1.3:-5804930 | MS.gene051622:CDS |
| ATCCTAGAAGGTGATCCATT+AGG | 0.507010 | 1.3:-5790866 | MS.gene051622:CDS |
| GTTTCAAGGTTGCTGGGCGA+AGG | 0.512547 | 1.3:-5790928 | MS.gene051622:intron |
| CCTGTGGCATTGTTTCCCAA+TGG | 0.518965 | 1.3:-5805329 | MS.gene051622:CDS |
| AACTTGCGAAGGAGAGAGGA+TGG | 0.521425 | 1.3:-5805168 | MS.gene051622:CDS |
| CAATGTTGCCAAGATAATTG+AGG | 0.525266 | 1.3:-5805676 | MS.gene051622:intron |
| GATACATGAAGTCCAGTGCT+AGG | 0.527724 | 1.3:-5789947 | MS.gene051622:CDS |
| GCTGGAACTGACATCCTAGA+AGG | 0.531175 | 1.3:-5790878 | MS.gene051622:CDS |
| CGAAGTAGAGTTTGGTAGAG+AGG | 0.537218 | 1.3:+5807112 | None:intergenic |
| TGATCCATTAGGAAGGTTGA+AGG | 0.537244 | 1.3:-5790855 | MS.gene051622:intron |
| TCTATTGGCCATACTATCAT+CGG | 0.540934 | 1.3:+5804884 | None:intergenic |
| TTATACTAATGGCAGGTTGG+AGG | 0.547276 | 1.3:-5805208 | MS.gene051622:intron |
| GAGAGGATGGGCCATTAATG+TGG | 0.551476 | 1.3:-5805155 | MS.gene051622:CDS |
| TCTCTAAGAGCAATTCCATC+GGG | 0.554567 | 1.3:+5790636 | None:intergenic |
| ATCTCTAAGAGCAATTCCAT+CGG | 0.557639 | 1.3:+5790635 | None:intergenic |
| GCGAAACTTGCGAAGGAGAG+AGG | 0.559522 | 1.3:-5805172 | MS.gene051622:CDS |
| CCCCATTTAGATGAATCAAA+CGG | 0.560065 | 1.3:+5806837 | None:intergenic |
| GCATTATAAACTAACAACTC+AGG | 0.561437 | 1.3:+5790899 | None:intergenic |
| ATCATACTTGAATAGTCTGA+AGG | 0.568219 | 1.3:-5805706 | MS.gene051622:CDS |
| ATCTGCGATGACCCTAGCAC+TGG | 0.569570 | 1.3:+5789935 | None:intergenic |
| TTACCAAAGGATATATTCCA+GGG | 0.571772 | 1.3:+5804763 | None:intergenic |
| TGATGTGTTTCAAGGTTGCT+GGG | 0.586550 | 1.3:-5790934 | MS.gene051622:intron |
| TGGGCCATTAATGTGGGAGG+AGG | 0.588432 | 1.3:-5805148 | MS.gene051622:CDS |
| AGAGGATGGGCCATTAATGT+GGG | 0.590581 | 1.3:-5805154 | MS.gene051622:CDS |
| TGCATGCAGATTAGTCCCGA+TGG | 0.591142 | 1.3:-5790651 | MS.gene051622:intron |
| TGTTCTGCAGAAAATGGTGG+TGG | 0.609323 | 1.3:-5805115 | MS.gene051622:CDS |
| TCATCTTTAGAAGCTTCAAG+AGG | 0.609813 | 1.3:+5806756 | None:intergenic |
| TTGGGAAACAATGCCACAGG+AGG | 0.609991 | 1.3:+5805332 | None:intergenic |
| TCGTCGGAAGCGGAACAAGA+AGG | 0.614373 | 1.3:+5807156 | None:intergenic |
| TTGGTTGTTACTTTAGAGGG+AGG | 0.615819 | 1.3:+5807084 | None:intergenic |
| CCATTGGGAAACAATGCCAC+AGG | 0.616536 | 1.3:+5805329 | None:intergenic |
| AAATACTTGATAGAAACAAG+TGG | 0.617582 | 1.3:-5789891 | MS.gene051622:CDS |
| CATTATAAACTAACAACTCA+GGG | 0.617843 | 1.3:+5790900 | None:intergenic |
| ATACATGAAGTCCAGTGCTA+GGG | 0.620185 | 1.3:-5789946 | MS.gene051622:CDS |
| TTCTAAAGATGATCTTCTAG+TGG | 0.620264 | 1.3:-5806745 | MS.gene051622:intron |
| TAGAAGGTGATCCATTAGGA+AGG | 0.638906 | 1.3:-5790862 | MS.gene051622:CDS |
| CCGTTTGATTCATCTAAATG+GGG | 0.639673 | 1.3:-5806837 | MS.gene051622:CDS |
| GTTGCGCATGAGAGTGTCGT+CGG | 0.640150 | 1.3:+5807140 | None:intergenic |
| CAAGAAGGAGGCGAAGACAT+CGG | 0.642374 | 1.3:+5807171 | None:intergenic |
| ACTTGCGAAGGAGAGAGGAT+GGG | 0.644649 | 1.3:-5805167 | MS.gene051622:CDS |
| ATTCTGGATATGTATAACCC+TGG | 0.648113 | 1.3:-5804780 | MS.gene051622:CDS |
| GAAATTAGATGAAGCACTCG+AGG | 0.655013 | 1.3:-5791328 | MS.gene051622:intron |
| TAAGAATCGGAGTAGATAAG+CGG | 0.660736 | 1.3:+5806980 | None:intergenic |
| ACATATCCAGAATGTAGACT+CGG | 0.660904 | 1.3:+5804790 | None:intergenic |
| GGATGGGCCATTAATGTGGG+AGG | 0.673443 | 1.3:-5805151 | MS.gene051622:CDS |
| TGGGAAACAATGCCACAGGA+GGG | 0.674322 | 1.3:+5805333 | None:intergenic |
| TCGGAAGCGGAACAAGAAGG+AGG | 0.682976 | 1.3:+5807159 | None:intergenic |
| CACTGTTCTGCAGAAAATGG+TGG | 0.684154 | 1.3:-5805118 | MS.gene051622:CDS |
| ATTGGCTGCGAAACTTGCGA+AGG | 0.694927 | 1.3:-5805179 | MS.gene051622:CDS |
| TCAACTGAACAAAAGCAAAG+CGG | 0.698918 | 1.3:+5805059 | None:intergenic |
| ATAAATATAGGTCCCTCCTG+TGG | 0.699886 | 1.3:-5805345 | MS.gene051622:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 5789872 | 5807190 | 5789872 | ID=MS.gene051622 |
| chr1.3 | mRNA | 5789872 | 5807190 | 5789872 | ID=MS.gene051622.t1;Parent=MS.gene051622 |
| chr1.3 | exon | 5807095 | 5807190 | 5807095 | ID=MS.gene051622.t1.exon1;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5807095 | 5807190 | 5807095 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5806950 | 5807005 | 5806950 | ID=MS.gene051622.t1.exon2;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5806950 | 5807005 | 5806950 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5806746 | 5806863 | 5806746 | ID=MS.gene051622.t1.exon3;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5806746 | 5806863 | 5806746 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5805677 | 5805739 | 5805677 | ID=MS.gene051622.t1.exon4;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5805677 | 5805739 | 5805677 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5805289 | 5805357 | 5805289 | ID=MS.gene051622.t1.exon5;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5805289 | 5805357 | 5805289 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5805049 | 5805215 | 5805049 | ID=MS.gene051622.t1.exon6;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5805049 | 5805215 | 5805049 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5804898 | 5804962 | 5804898 | ID=MS.gene051622.t1.exon7;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5804898 | 5804962 | 5804898 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5804767 | 5804813 | 5804767 | ID=MS.gene051622.t1.exon8;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5804767 | 5804813 | 5804767 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5791475 | 5791519 | 5791475 | ID=MS.gene051622.t1.exon9;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5791475 | 5791519 | 5791475 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5791329 | 5791379 | 5791329 | ID=MS.gene051622.t1.exon10;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5791329 | 5791379 | 5791329 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5790856 | 5790942 | 5790856 | ID=MS.gene051622.t1.exon11;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5790856 | 5790942 | 5790856 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5790577 | 5790664 | 5790577 | ID=MS.gene051622.t1.exon12;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5790577 | 5790664 | 5790577 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| chr1.3 | exon | 5789872 | 5789972 | 5789872 | ID=MS.gene051622.t1.exon13;Parent=MS.gene051622.t1 |
| chr1.3 | CDS | 5789872 | 5789972 | 5789872 | ID=cds.MS.gene051622.t1;Parent=MS.gene051622.t1 |
| Gene Sequence |
| Protein sequence |
