AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051827

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051827.t1 MTR_4g125700 98.819 508 6 0 1 508 1 508 0.0 1039
MS.gene051827.t1 MTR_2g099510 83.441 465 76 1 45 508 66 530 0.0 817
MS.gene051827.t1 MTR_8g052550 46.721 122 18 4 1 122 1 75 1.12e-22 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051827.t1 AT4G29840 77.099 524 97 7 1 504 1 521 0.0 811
MS.gene051827.t1 AT1G72810 81.545 466 81 3 41 504 51 513 0.0 792

Find 142 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAATGTGTAAAGATTT+AGG 0.134689 8.2:-6956596 MS.gene051827:CDS
TTCTGAATTGCCGATATATT+TGG 0.177867 8.2:-6956759 MS.gene051827:CDS
AGTAAGATTGATTATCATTC+TGG 0.199505 8.2:-6956194 MS.gene051827:CDS
CTCAGGAATAATGGGGTTAT+TGG 0.245982 8.2:-6956272 MS.gene051827:CDS
ATTGTACCTGGTGGAAATTT+AGG 0.246107 8.2:-6956644 MS.gene051827:CDS
ATATCATCAGGGTGGATTTC+TGG 0.247855 8.2:+6957169 None:intergenic
TTGATTAAACTCAGGAATAA+TGG 0.260884 8.2:-6956281 MS.gene051827:CDS
GTGAAGTTGTTGGCGGTGGC+AGG 0.266465 8.2:+6957418 None:intergenic
TCGTCGAGGGAGTAGGATTC+AGG 0.266996 8.2:+6957358 None:intergenic
GTGTCTGTTAAGGCTGATTT+TGG 0.270510 8.2:-6956134 MS.gene051827:CDS
TCAAAATGTGTAAAGATTTA+GGG 0.279636 8.2:-6956595 MS.gene051827:CDS
GGAGTTGAATGGGACGTATT+TGG 0.279906 8.2:+6957393 None:intergenic
ATGGTTGTATGAAGCTGATT+AGG 0.316364 8.2:-6956790 MS.gene051827:CDS
ACCACTTGGCCTTACGGTTC+AGG 0.317729 8.2:-6957220 MS.gene051827:CDS
TTGTAGGCATGAACGATCTT+TGG 0.318466 8.2:-6957087 MS.gene051827:CDS
TGATTAAACTCAGGAATAAT+GGG 0.322866 8.2:-6956280 MS.gene051827:CDS
GGCCTTACGGTTCAGGTGTA+TGG 0.326197 8.2:-6957213 MS.gene051827:CDS
TTGTACCTGGTGGAAATTTA+GGG 0.337501 8.2:-6956643 MS.gene051827:CDS
TTCAATTCGTAAACTATTCA+AGG 0.352361 8.2:+6956730 None:intergenic
CGGCGGTTATGTGTGGGTTT+AGG 0.354555 8.2:+6957532 None:intergenic
CACTGTGGTAATAGCCATAC+TGG 0.361097 8.2:-6957058 MS.gene051827:CDS
GATCTTTGAAGCTTCCAGTA+TGG 0.386403 8.2:+6957044 None:intergenic
CGAAGTTCGACGGCGCGTAC+TGG 0.391694 8.2:-6957273 MS.gene051827:CDS
ACTGGAAGCTTCAAAGATCT+TGG 0.393842 8.2:-6957040 MS.gene051827:CDS
TGTAGGCATGAACGATCTTT+GGG 0.399165 8.2:-6957086 MS.gene051827:CDS
TTGTATTTGTATTATAAGAA+TGG 0.406563 8.2:-6956518 MS.gene051827:CDS
TCTGCTTATTGCGCTTCAGC+TGG 0.409965 8.2:-6956917 MS.gene051827:CDS
TATCATTCTGGGAATATTGC+TGG 0.410252 8.2:-6956182 MS.gene051827:CDS
AAGGAATTCGCCAAATATAT+CGG 0.411303 8.2:+6956749 None:intergenic
ATGTTTATTTGTCCTCATAC+TGG 0.412669 8.2:-6956320 MS.gene051827:CDS
ATCCATCAATTCCTCTTCAC+TGG 0.414822 8.2:+6956370 None:intergenic
TTGAATAGTTTACGAATTGA+AGG 0.416111 8.2:-6956728 MS.gene051827:CDS
TCTGGGAATATTGCTGGTAT+TGG 0.416558 8.2:-6956176 MS.gene051827:CDS
ATGGCACTTGCTGATTCAAC+TGG 0.418295 8.2:-6956344 MS.gene051827:CDS
TTTGCTTCTGCTATTCAGAT+TGG 0.423112 8.2:-6956458 MS.gene051827:CDS
AGCCAGTGAAGAGGAATTGA+TGG 0.425665 8.2:-6956372 MS.gene051827:CDS
AATGACTGTGCTTGTTAGTC+AGG 0.431806 8.2:-6957017 MS.gene051827:CDS
GGGAAAACCACTTGGCCTTA+CGG 0.436987 8.2:-6957226 MS.gene051827:CDS
TTCAGGTGTATGGAGCAAAA+AGG 0.437179 8.2:-6957203 MS.gene051827:CDS
AAAGCGCTAACGATATCATC+AGG 0.438146 8.2:+6957157 None:intergenic
TTTGCAGCAGTTTGATTGGC+AGG 0.441607 8.2:-6956681 MS.gene051827:CDS
TGGTTGTATGAAGCTGATTA+GGG 0.444344 8.2:-6956789 MS.gene051827:CDS
TAATCCACCTGTGTCTGTTA+AGG 0.451369 8.2:-6956144 MS.gene051827:CDS
GGCGATGTCTTTGATGTTGT+CGG 0.451433 8.2:+6957450 None:intergenic
GAGTCGAAGGTGGAGTTGAA+TGG 0.451902 8.2:+6957382 None:intergenic
GTATTTGGCGGTGAAGTTGT+TGG 0.463189 8.2:+6957408 None:intergenic
ACAGCTCTATCAATTGAAAC+AGG 0.468555 8.2:+6956431 None:intergenic
GTAAGATTGATTATCATTCT+GGG 0.468628 8.2:-6956193 MS.gene051827:CDS
ATTTGTATTATAAGAATGGT+TGG 0.468821 8.2:-6956514 MS.gene051827:CDS
TGTTGGCGGTGGCAGGGTGA+CGG 0.469152 8.2:+6957425 None:intergenic
AGTCGAAGGTGGAGTTGAAT+GGG 0.469459 8.2:+6957383 None:intergenic
ATCGTCTACCGCAGCAACTC+CGG 0.471571 8.2:-6957334 MS.gene051827:CDS
GGCGGTGGCAGGGTGACGGC+GGG 0.472151 8.2:+6957429 None:intergenic
AACCTCTTCGACTCGCGTGT+GGG 0.484266 8.2:-6957247 MS.gene051827:CDS
CCAGGTACAATCACCCAATC+AGG 0.486304 8.2:+6956656 None:intergenic
AGAGTGGTTGTTGGTGGTAG+CGG 0.486794 8.2:+6957492 None:intergenic
GGAGTTGGTTGTGCTTCCAC+CGG 0.488070 8.2:-6956959 MS.gene051827:CDS
AACCGTCCGTTGGTCGGAGT+TGG 0.488563 8.2:-6956974 MS.gene051827:CDS
TTGATTGGCAGGTACCTGAT+TGG 0.489621 8.2:-6956670 MS.gene051827:CDS
CGCGTGTGGGGAAAACCACT+TGG 0.493520 8.2:-6957234 MS.gene051827:CDS
CCTGATTGGGTGATTGTACC+TGG 0.494816 8.2:-6956656 MS.gene051827:CDS
ATGTTGTCGGTGGCGAGATC+CGG 0.497930 8.2:+6957463 None:intergenic
TGATTGGCAGGTACCTGATT+GGG 0.499461 8.2:-6956669 MS.gene051827:CDS
TGGAGCCAGTGAGAGGGTTG+TGG 0.505753 8.2:-6956252 MS.gene051827:CDS
CAACCTCTTCGACTCGCGTG+TGG 0.509904 8.2:-6957248 MS.gene051827:CDS
TTGATTCAACCGGTATCAAA+TGG 0.513680 8.2:-6956845 MS.gene051827:CDS
GTGGTTGTTAGTACTGCTCA+TGG 0.517105 8.2:-6956233 MS.gene051827:CDS
GGCGGTGAAGTTGTTGGCGG+TGG 0.517738 8.2:+6957414 None:intergenic
ACCAATGCACCATTTGATAC+CGG 0.522774 8.2:+6956836 None:intergenic
CGGTGATGGAGAGTGGTTGT+TGG 0.523963 8.2:+6957483 None:intergenic
CGTCGAGGGAGTAGGATTCA+GGG 0.524958 8.2:+6957359 None:intergenic
TGAAGTTGTTGGCGGTGGCA+GGG 0.527974 8.2:+6957419 None:intergenic
TCGCGAGTGCTTCGATGTCA+TGG 0.529208 8.2:+6957293 None:intergenic
AACCGCGCAGTTGATTCAAC+CGG 0.530203 8.2:-6956855 MS.gene051827:CDS
GTTAAAGCCACACCAGTATG+AGG 0.530207 8.2:+6956308 None:intergenic
ACCGGTATCAAATGGTGCAT+TGG 0.530776 8.2:-6956837 MS.gene051827:CDS
TGGCGGTGGCAGGGTGACGG+CGG 0.530809 8.2:+6957428 None:intergenic
TGTTGATAAGATACCAAGGC+TGG 0.532452 8.2:-6956570 MS.gene051827:CDS
GTTGGTGGTAGCGGTGGCGA+CGG 0.534404 8.2:+6957501 None:intergenic
TAACTGCTTTGATTAAACTC+AGG 0.539179 8.2:-6956289 MS.gene051827:CDS
GACGATTTCGTCGAGGGAGT+AGG 0.540791 8.2:+6957351 None:intergenic
AACAACCACAACCCTCTCAC+TGG 0.543564 8.2:+6956247 None:intergenic
AAAACGATGGTGGAGAATGA+AGG 0.547108 8.2:+6957562 None:intergenic
ACCTCTTCGACTCGCGTGTG+GGG 0.547841 8.2:-6957246 MS.gene051827:CDS
GCTTGTGCACAAACCAGCCT+TGG 0.551031 8.2:+6956557 None:intergenic
TGCTTCCACCGGTGATACAT+CGG 0.553305 8.2:-6956948 MS.gene051827:CDS
TTAGTCAGGTAAATCGCCTC+CGG 0.556393 8.2:-6957003 MS.gene051827:CDS
TATGCATTGAAGAATTCTGA+TGG 0.567257 8.2:-6956407 MS.gene051827:CDS
GCGGTAGACGATTTCGTCGA+GGG 0.570561 8.2:+6957345 None:intergenic
GTGGACGTCGAGAAGGCCGC+CGG 0.570710 8.2:+6957315 None:intergenic
CAGCGCAGCCGATGTATCAC+CGG 0.572600 8.2:+6956940 None:intergenic
TCCCCACACGCGAGTCGAAG+AGG 0.573166 8.2:+6957245 None:intergenic
GGATTCAGGGGAGTCGAAGG+TGG 0.574210 8.2:+6957372 None:intergenic
ATGCATTGAAGAATTCTGAT+GGG 0.574216 8.2:-6956406 MS.gene051827:CDS
TATTTGTCCTCATACTGGTG+TGG 0.574702 8.2:-6956315 MS.gene051827:CDS
CAACAACCACTCTCCATCAC+CGG 0.575317 8.2:-6957482 MS.gene051827:CDS
GAAGCACTCGCGAAGTTCGA+CGG 0.577029 8.2:-6957283 MS.gene051827:CDS
GGGTTATTGGAGCCAGTGAG+AGG 0.577743 8.2:-6956259 MS.gene051827:CDS
GATTAAACTCAGGAATAATG+GGG 0.580133 8.2:-6956279 MS.gene051827:CDS
GAGTGTGCGGCGGTTATGTG+TGG 0.580993 8.2:+6957525 None:intergenic
GTAGGATTCAGGGGAGTCGA+AGG 0.587464 8.2:+6957369 None:intergenic
CCTTGGACTACAAACTACTT+CGG 0.589244 8.2:+6956068 None:intergenic
AAAATGAACCGTCCGTTGGT+CGG 0.591612 8.2:-6956980 MS.gene051827:CDS
CGGTGGCGACGGTGAGTGTG+CGG 0.594405 8.2:+6957512 None:intergenic
TTCTTATAATACAAATACAA+AGG 0.596623 8.2:+6956521 None:intergenic
CTCCATACACCTGAACCGTA+AGG 0.596883 8.2:+6957211 None:intergenic
CGAGATCCGGTGATGGAGAG+TGG 0.597957 8.2:+6957476 None:intergenic
TGATGGAGAGTGGTTGTTGG+TGG 0.599110 8.2:+6957486 None:intergenic
GCACAACCAACTCCGACCAA+CGG 0.599617 8.2:+6956968 None:intergenic
CGCAGCCGATGTATCACCGG+TGG 0.601314 8.2:+6956943 None:intergenic
TGTCATGGTGGACGTCGAGA+AGG 0.606780 8.2:+6957308 None:intergenic
TCGGTGGCGAGATCCGGTGA+TGG 0.607772 8.2:+6957469 None:intergenic
TGTTGAGGAAGCCAGTGAAG+AGG 0.612541 8.2:-6956381 MS.gene051827:CDS
CCGGAAAATGAACCGTCCGT+TGG 0.613505 8.2:-6956984 MS.gene051827:CDS
GAAGGCCGCCGGAGTTGCTG+CGG 0.613672 8.2:+6957326 None:intergenic
AACCAACTCCGACCAACGGA+CGG 0.618009 8.2:+6956972 None:intergenic
AGTGTGCGGCGGTTATGTGT+GGG 0.619041 8.2:+6957526 None:intergenic
GTGGTTGTTGGTGGTAGCGG+TGG 0.620617 8.2:+6957495 None:intergenic
GAATTCTGATGGGATTGTTG+AGG 0.621403 8.2:-6956396 MS.gene051827:CDS
AAATCAGCCTTAACAGACAC+AGG 0.623027 8.2:+6956137 None:intergenic
GTCGAGGGAGTAGGATTCAG+GGG 0.625447 8.2:+6957360 None:intergenic
TTTGGCGGTGAAGTTGTTGG+CGG 0.626032 8.2:+6957411 None:intergenic
GTTGAATGGGACGTATTTGG+CGG 0.632315 8.2:+6957396 None:intergenic
CATCAGGGTGGATTTCTGGT+AGG 0.634205 8.2:+6957173 None:intergenic
GGTTATTGGAGCCAGTGAGA+GGG 0.634474 8.2:-6956258 MS.gene051827:CDS
TGCGGTAGACGATTTCGTCG+AGG 0.635297 8.2:+6957344 None:intergenic
ACCTGAACCGTAAGGCCAAG+TGG 0.644246 8.2:+6957219 None:intergenic
AGAGTTTAAAGCGATTAAAG+CGG 0.650024 8.2:-6956489 MS.gene051827:CDS
TGGCGACGGTGAGTGTGCGG+CGG 0.651305 8.2:+6957515 None:intergenic
CGCTAACGATATCATCAGGG+TGG 0.654724 8.2:+6957161 None:intergenic
ATATTCCCTAAATTTCCACC+AGG 0.655231 8.2:+6956638 None:intergenic
AGAGGAATTGATGGATGCAA+TGG 0.657723 8.2:-6956363 MS.gene051827:CDS
GGCTTGTTGATAAGATACCA+AGG 0.657922 8.2:-6956574 MS.gene051827:CDS
GCTGGTAGGAAAACAATCGA+AGG 0.673402 8.2:+6956890 None:intergenic
GATGTCTTTGATGTTGTCGG+TGG 0.677706 8.2:+6957453 None:intergenic
GTCTACCGCAGCAACTCCGG+CGG 0.686741 8.2:-6957331 MS.gene051827:CDS
AAGCGCTAACGATATCATCA+GGG 0.689325 8.2:+6957158 None:intergenic
TCAGCCTTAACAGACACAGG+TGG 0.717631 8.2:+6956140 None:intergenic
CGAGTGCTTCGATGTCATGG+TGG 0.720420 8.2:+6957296 None:intergenic
GATCTTTGGGTTAAACACTG+TGG 0.724956 8.2:-6957073 MS.gene051827:CDS
TACCGGTTGAATCAACTGCG+CGG 0.770329 8.2:+6956853 None:intergenic
GATTGGGTGATTGTACCTGG+TGG 0.786775 8.2:-6956653 MS.gene051827:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTATAATACAAATACAA+AGG + chr8.2:6957150-6957169 None:intergenic 15.0%
!! TTGTATTTGTATTATAAGAA+TGG - chr8.2:6957150-6957169 MS.gene051827:CDS 15.0%
!!! AATATTTATGCATTTTACAA+AGG - chr8.2:6957048-6957067 MS.gene051827:CDS 15.0%
!! ATTTGTATTATAAGAATGGT+TGG - chr8.2:6957154-6957173 MS.gene051827:CDS 20.0%
!! TCAAAATGTGTAAAGATTTA+GGG - chr8.2:6957073-6957092 MS.gene051827:CDS 20.0%
!! TTCAAAATGTGTAAAGATTT+AGG - chr8.2:6957072-6957091 MS.gene051827:CDS 20.0%
! AGTAAGATTGATTATCATTC+TGG - chr8.2:6957474-6957493 MS.gene051827:CDS 25.0%
! GTAAGATTGATTATCATTCT+GGG - chr8.2:6957475-6957494 MS.gene051827:CDS 25.0%
! TGATTAAACTCAGGAATAAT+GGG - chr8.2:6957388-6957407 MS.gene051827:CDS 25.0%
! TTCAATTCGTAAACTATTCA+AGG + chr8.2:6956941-6956960 None:intergenic 25.0%
! TTGAATAGTTTACGAATTGA+AGG - chr8.2:6956940-6956959 MS.gene051827:CDS 25.0%
! TTGATTAAACTCAGGAATAA+TGG - chr8.2:6957387-6957406 MS.gene051827:CDS 25.0%
!!! AGTATTGATACTGATTTTGA+TGG - chr8.2:6956859-6956878 MS.gene051827:CDS 25.0%
AAGGAATTCGCCAAATATAT+CGG + chr8.2:6956922-6956941 None:intergenic 30.0%
ATGCATTGAAGAATTCTGAT+GGG - chr8.2:6957262-6957281 MS.gene051827:CDS 30.0%
ATGTTTATTTGTCCTCATAC+TGG - chr8.2:6957348-6957367 MS.gene051827:CDS 30.0%
GATTAAACTCAGGAATAATG+GGG - chr8.2:6957389-6957408 MS.gene051827:CDS 30.0%
TAACTGCTTTGATTAAACTC+AGG - chr8.2:6957379-6957398 MS.gene051827:CDS 30.0%
TATGCATTGAAGAATTCTGA+TGG - chr8.2:6957261-6957280 MS.gene051827:CDS 30.0%
TTCTGAATTGCCGATATATT+TGG - chr8.2:6956909-6956928 MS.gene051827:CDS 30.0%
! AAGGTAACTCTAATCTTTTC+TGG - chr8.2:6956536-6956555 MS.gene051827:CDS 30.0%
! AGAGTTTAAAGCGATTAAAG+CGG - chr8.2:6957179-6957198 MS.gene051827:CDS 30.0%
! AGGTAACTCTAATCTTTTCT+GGG - chr8.2:6956537-6956556 MS.gene051827:CDS 30.0%
ACAGCTCTATCAATTGAAAC+AGG + chr8.2:6957240-6957259 None:intergenic 35.0%
ATATTCCCTAAATTTCCACC+AGG + chr8.2:6957033-6957052 None:intergenic 35.0%
ATGGTTGTATGAAGCTGATT+AGG - chr8.2:6956878-6956897 MS.gene051827:CDS 35.0%
ATTGTACCTGGTGGAAATTT+AGG - chr8.2:6957024-6957043 MS.gene051827:CDS 35.0%
TGGTTGTATGAAGCTGATTA+GGG - chr8.2:6956879-6956898 MS.gene051827:CDS 35.0%
TGTATGGAGCAAAAAGGAAT+GGG - chr8.2:6956471-6956490 MS.gene051827:CDS 35.0%
TTGATTCAACCGGTATCAAA+TGG - chr8.2:6956823-6956842 MS.gene051827:CDS 35.0%
TTGTACCTGGTGGAAATTTA+GGG - chr8.2:6957025-6957044 MS.gene051827:CDS 35.0%
TTTGCTTCTGCTATTCAGAT+TGG - chr8.2:6957210-6957229 MS.gene051827:CDS 35.0%
! AGATTTTGCAGCAGTTTGAT+TGG - chr8.2:6956983-6957002 MS.gene051827:CDS 35.0%
! TATCATTCTGGGAATATTGC+TGG - chr8.2:6957486-6957505 MS.gene051827:CDS 35.0%
! TTGAGATTTTATTCGCTGGT+AGG + chr8.2:6956795-6956814 None:intergenic 35.0%
!! TTTAGGAGCGTTAAAAACGA+TGG + chr8.2:6956122-6956141 None:intergenic 35.0%
AAATCAGCCTTAACAGACAC+AGG + chr8.2:6957534-6957553 None:intergenic 40.0%
AAGCGCTAACGATATCATCA+GGG + chr8.2:6956513-6956532 None:intergenic 40.0%
ACCAATGCACCATTTGATAC+CGG + chr8.2:6956835-6956854 None:intergenic 40.0%
ACTGGAAGCTTCAAAGATCT+TGG - chr8.2:6956628-6956647 MS.gene051827:CDS 40.0%
ATCCATCAATTCCTCTTCAC+TGG + chr8.2:6957301-6957320 None:intergenic 40.0%
GAATTCTGATGGGATTGTTG+AGG - chr8.2:6957272-6957291 MS.gene051827:CDS 40.0%
GTGTATGGAGCAAAAAGGAA+TGG - chr8.2:6956470-6956489 MS.gene051827:CDS 40.0%
TAATCCACCTGTGTCTGTTA+AGG - chr8.2:6957524-6957543 MS.gene051827:CDS 40.0%
TATTTGTCCTCATACTGGTG+TGG - chr8.2:6957353-6957372 MS.gene051827:CDS 40.0%
TCTGGGAATATTGCTGGTAT+TGG - chr8.2:6957492-6957511 MS.gene051827:CDS 40.0%
TGTAGGCATGAACGATCTTT+GGG - chr8.2:6956582-6956601 MS.gene051827:CDS 40.0%
TTCAGGTGTATGGAGCAAAA+AGG - chr8.2:6956465-6956484 MS.gene051827:CDS 40.0%
TTGTAGGCATGAACGATCTT+TGG - chr8.2:6956581-6956600 MS.gene051827:CDS 40.0%
! AAAGCGCTAACGATATCATC+AGG + chr8.2:6956514-6956533 None:intergenic 40.0%
! AATGACTGTGCTTGTTAGTC+AGG - chr8.2:6956651-6956670 MS.gene051827:CDS 40.0%
! AGAGGAATTGATGGATGCAA+TGG - chr8.2:6957305-6957324 MS.gene051827:CDS 40.0%
! ATATCATCAGGGTGGATTTC+TGG + chr8.2:6956502-6956521 None:intergenic 40.0%
! CTCAGGAATAATGGGGTTAT+TGG - chr8.2:6957396-6957415 MS.gene051827:CDS 40.0%
! GATATCGTTAGCGCTTTTGA+AGG - chr8.2:6956517-6956536 MS.gene051827:CDS 40.0%
! GATCTTTGGGTTAAACACTG+TGG - chr8.2:6956595-6956614 MS.gene051827:CDS 40.0%
! TGTTGATAAGATACCAAGGC+TGG - chr8.2:6957098-6957117 MS.gene051827:CDS 40.0%
!! AAAACGATGGTGGAGAATGA+AGG + chr8.2:6956109-6956128 None:intergenic 40.0%
!! CGTTTTGGCAAACAGTTTGT+AGG - chr8.2:6956565-6956584 MS.gene051827:CDS 40.0%
!! GATCTTTGAAGCTTCCAGTA+TGG + chr8.2:6956627-6956646 None:intergenic 40.0%
!! GGCTTGTTGATAAGATACCA+AGG - chr8.2:6957094-6957113 MS.gene051827:CDS 40.0%
!! GTGTCTGTTAAGGCTGATTT+TGG - chr8.2:6957534-6957553 MS.gene051827:CDS 40.0%
!!! GGCTGATTTTGGTTCTGTTA+TGG - chr8.2:6957545-6957564 MS.gene051827:CDS 40.0%
AAAATGAACCGTCCGTTGGT+CGG - chr8.2:6956688-6956707 MS.gene051827:CDS 45.0%
ACCGGTATCAAATGGTGCAT+TGG - chr8.2:6956831-6956850 MS.gene051827:CDS 45.0%
AGCCAGTGAAGAGGAATTGA+TGG - chr8.2:6957296-6957315 MS.gene051827:CDS 45.0%
AGTCGAAGGTGGAGTTGAAT+GGG + chr8.2:6956288-6956307 None:intergenic 45.0%
CACTGTGGTAATAGCCATAC+TGG - chr8.2:6956610-6956629 MS.gene051827:CDS 45.0%
GATGTCTTTGATGTTGTCGG+TGG + chr8.2:6956218-6956237 None:intergenic 45.0%
GCTGGTAGGAAAACAATCGA+AGG + chr8.2:6956781-6956800 None:intergenic 45.0%
GGAGTTGAATGGGACGTATT+TGG + chr8.2:6956278-6956297 None:intergenic 45.0%
GGCGATGTCTTTGATGTTGT+CGG + chr8.2:6956221-6956240 None:intergenic 45.0%
GTATTTGGCGGTGAAGTTGT+TGG + chr8.2:6956263-6956282 None:intergenic 45.0%
GTGGTTGTTAGTACTGCTCA+TGG - chr8.2:6957435-6957454 MS.gene051827:CDS 45.0%
GTTAAAGCCACACCAGTATG+AGG + chr8.2:6957363-6957382 None:intergenic 45.0%
GTTGAATGGGACGTATTTGG+CGG + chr8.2:6956275-6956294 None:intergenic 45.0%
TGATTGGCAGGTACCTGATT+GGG - chr8.2:6956999-6957018 MS.gene051827:CDS 45.0%
TTAGTCAGGTAAATCGCCTC+CGG - chr8.2:6956665-6956684 MS.gene051827:CDS 45.0%
TTGATTGGCAGGTACCTGAT+TGG - chr8.2:6956998-6957017 MS.gene051827:CDS 45.0%
TTTGCAGCAGTTTGATTGGC+AGG - chr8.2:6956987-6957006 MS.gene051827:CDS 45.0%
! CTTTTCTGGGCTGAACGTTT+TGG - chr8.2:6956550-6956569 MS.gene051827:CDS 45.0%
! GCGGTTGAGATTTTATTCGC+TGG + chr8.2:6956799-6956818 None:intergenic 45.0%
!! AGGAGCGTTAAAAACGATGG+TGG + chr8.2:6956119-6956138 None:intergenic 45.0%
!! ATGGCACTTGCTGATTCAAC+TGG - chr8.2:6957324-6957343 MS.gene051827:CDS 45.0%
AACAACCACAACCCTCTCAC+TGG + chr8.2:6957424-6957443 None:intergenic 50.0%
CAACAACCACTCTCCATCAC+CGG - chr8.2:6956186-6956205 MS.gene051827:CDS 50.0%
CCAGGTACAATCACCCAATC+AGG + chr8.2:6957015-6957034 None:intergenic 50.0%
CCTGATTGGGTGATTGTACC+TGG - chr8.2:6957012-6957031 MS.gene051827:CDS 50.0%
CGCTAACGATATCATCAGGG+TGG + chr8.2:6956510-6956529 None:intergenic 50.0%
CTCCATACACCTGAACCGTA+AGG + chr8.2:6956460-6956479 None:intergenic 50.0%
GAGTCGAAGGTGGAGTTGAA+TGG + chr8.2:6956289-6956308 None:intergenic 50.0%
GATTGGGTGATTGTACCTGG+TGG - chr8.2:6957015-6957034 MS.gene051827:CDS 50.0%
GGTTATTGGAGCCAGTGAGA+GGG - chr8.2:6957410-6957429 MS.gene051827:CDS 50.0%
TCAGCCTTAACAGACACAGG+TGG + chr8.2:6957531-6957550 None:intergenic 50.0%
TCTGCTTATTGCGCTTCAGC+TGG - chr8.2:6956751-6956770 MS.gene051827:CDS 50.0%
TGATGGAGAGTGGTTGTTGG+TGG + chr8.2:6956185-6956204 None:intergenic 50.0%
TGCTTCCACCGGTGATACAT+CGG - chr8.2:6956720-6956739 MS.gene051827:CDS 50.0%
TGTTGAGGAAGCCAGTGAAG+AGG - chr8.2:6957287-6957306 MS.gene051827:CDS 50.0%
TTTGGCGGTGAAGTTGTTGG+CGG + chr8.2:6956260-6956279 None:intergenic 50.0%
! AACCGCGCAGTTGATTCAAC+CGG - chr8.2:6956813-6956832 MS.gene051827:CDS 50.0%
! CCAACGGACGGTTCATTTTC+CGG + chr8.2:6956687-6956706 None:intergenic 50.0%
! TACCGGTTGAATCAACTGCG+CGG + chr8.2:6956818-6956837 None:intergenic 50.0%
!! AGAGTGGTTGTTGGTGGTAG+CGG + chr8.2:6956179-6956198 None:intergenic 50.0%
!! CATCAGGGTGGATTTCTGGT+AGG + chr8.2:6956498-6956517 None:intergenic 50.0%
!! GGGAAAACCACTTGGCCTTA+CGG - chr8.2:6956442-6956461 MS.gene051827:CDS 50.0%
AACCAACTCCGACCAACGGA+CGG + chr8.2:6956699-6956718 None:intergenic 55.0%
ACCTGAACCGTAAGGCCAAG+TGG + chr8.2:6956452-6956471 None:intergenic 55.0%
AGTGTGCGGCGGTTATGTGT+GGG + chr8.2:6956145-6956164 None:intergenic 55.0%
ATCGTCTACCGCAGCAACTC+CGG - chr8.2:6956334-6956353 MS.gene051827:CDS 55.0%
ATGTTGTCGGTGGCGAGATC+CGG + chr8.2:6956208-6956227 None:intergenic 55.0%
CCGGAAAATGAACCGTCCGT+TGG - chr8.2:6956684-6956703 MS.gene051827:CDS 55.0%
CGAGTGCTTCGATGTCATGG+TGG + chr8.2:6956375-6956394 None:intergenic 55.0%
CGGCGGTTATGTGTGGGTTT+AGG + chr8.2:6956139-6956158 None:intergenic 55.0%
CGTCGAGGGAGTAGGATTCA+GGG + chr8.2:6956312-6956331 None:intergenic 55.0%
GACGATTTCGTCGAGGGAGT+AGG + chr8.2:6956320-6956339 None:intergenic 55.0%
GCACAACCAACTCCGACCAA+CGG + chr8.2:6956703-6956722 None:intergenic 55.0%
GCGGTAGACGATTTCGTCGA+GGG + chr8.2:6956326-6956345 None:intergenic 55.0%
GCTTGTGCACAAACCAGCCT+TGG + chr8.2:6957114-6957133 None:intergenic 55.0%
GGCCTTACGGTTCAGGTGTA+TGG - chr8.2:6956455-6956474 MS.gene051827:CDS 55.0%
GGGTTATTGGAGCCAGTGAG+AGG - chr8.2:6957409-6957428 MS.gene051827:CDS 55.0%
GTAGGATTCAGGGGAGTCGA+AGG + chr8.2:6956302-6956321 None:intergenic 55.0%
GTCGAGGGAGTAGGATTCAG+GGG + chr8.2:6956311-6956330 None:intergenic 55.0%
TCGCGAGTGCTTCGATGTCA+TGG + chr8.2:6956378-6956397 None:intergenic 55.0%
TCGTCGAGGGAGTAGGATTC+AGG + chr8.2:6956313-6956332 None:intergenic 55.0%
TGAAGTTGTTGGCGGTGGCA+GGG + chr8.2:6956252-6956271 None:intergenic 55.0%
TGCGGTAGACGATTTCGTCG+AGG + chr8.2:6956327-6956346 None:intergenic 55.0%
! AACCTCTTCGACTCGCGTGT+GGG - chr8.2:6956421-6956440 MS.gene051827:CDS 55.0%
! ACGGACGGTTCATTTTCCGG+AGG + chr8.2:6956684-6956703 None:intergenic 55.0%
! GAAGCACTCGCGAAGTTCGA+CGG - chr8.2:6956385-6956404 MS.gene051827:CDS 55.0%
! GGAGTTGGTTGTGCTTCCAC+CGG - chr8.2:6956709-6956728 MS.gene051827:CDS 55.0%
!! ACCACTTGGCCTTACGGTTC+AGG - chr8.2:6956448-6956467 MS.gene051827:CDS 55.0%
!! CGGTGATGGAGAGTGGTTGT+TGG + chr8.2:6956188-6956207 None:intergenic 55.0%
!! TGTCATGGTGGACGTCGAGA+AGG + chr8.2:6956363-6956382 None:intergenic 55.0%
AACCGTCCGTTGGTCGGAGT+TGG - chr8.2:6956694-6956713 MS.gene051827:CDS 60.0%
CAGCGCAGCCGATGTATCAC+CGG + chr8.2:6956731-6956750 None:intergenic 60.0%
CGCGTGTGGGGAAAACCACT+TGG - chr8.2:6956434-6956453 MS.gene051827:CDS 60.0%
GAGTGTGCGGCGGTTATGTG+TGG + chr8.2:6956146-6956165 None:intergenic 60.0%
GGATTCAGGGGAGTCGAAGG+TGG + chr8.2:6956299-6956318 None:intergenic 60.0%
GTGAAGTTGTTGGCGGTGGC+AGG + chr8.2:6956253-6956272 None:intergenic 60.0%
TGGAGCCAGTGAGAGGGTTG+TGG - chr8.2:6957416-6957435 MS.gene051827:CDS 60.0%
! ACCTCTTCGACTCGCGTGTG+GGG - chr8.2:6956422-6956441 MS.gene051827:CDS 60.0%
! CAACCTCTTCGACTCGCGTG+TGG - chr8.2:6956420-6956439 MS.gene051827:CDS 60.0%
!! CGAGATCCGGTGATGGAGAG+TGG + chr8.2:6956195-6956214 None:intergenic 60.0%
!! GTGGTTGTTGGTGGTAGCGG+TGG + chr8.2:6956176-6956195 None:intergenic 60.0%
CGAAGTTCGACGGCGCGTAC+TGG - chr8.2:6956395-6956414 MS.gene051827:CDS 65.0%
CGCAGCCGATGTATCACCGG+TGG + chr8.2:6956728-6956747 None:intergenic 65.0%
GGCGGTGAAGTTGTTGGCGG+TGG + chr8.2:6956257-6956276 None:intergenic 65.0%
GTCTACCGCAGCAACTCCGG+CGG - chr8.2:6956337-6956356 MS.gene051827:CDS 65.0%
TCCCCACACGCGAGTCGAAG+AGG + chr8.2:6956426-6956445 None:intergenic 65.0%
TCGGTGGCGAGATCCGGTGA+TGG + chr8.2:6956202-6956221 None:intergenic 65.0%
TGTTGGCGGTGGCAGGGTGA+CGG + chr8.2:6956246-6956265 None:intergenic 65.0%
!! GTTGGTGGTAGCGGTGGCGA+CGG + chr8.2:6956170-6956189 None:intergenic 65.0%
CGGTGGCGACGGTGAGTGTG+CGG + chr8.2:6956159-6956178 None:intergenic 70.0%
GAAGGCCGCCGGAGTTGCTG+CGG + chr8.2:6956345-6956364 None:intergenic 70.0%
GTGGACGTCGAGAAGGCCGC+CGG + chr8.2:6956356-6956375 None:intergenic 70.0%
TGGCGACGGTGAGTGTGCGG+CGG + chr8.2:6956156-6956175 None:intergenic 70.0%
! TGGCGGTGGCAGGGTGACGG+CGG + chr8.2:6956243-6956262 None:intergenic 75.0%
!! GGCGGTGGCAGGGTGACGGC+GGG + chr8.2:6956242-6956261 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 6956082 6957608 6956082 ID=MS.gene051827
chr8.2 mRNA 6956082 6957608 6956082 ID=MS.gene051827.t1;Parent=MS.gene051827
chr8.2 exon 6956082 6957608 6956082 ID=MS.gene051827.t1.exon1;Parent=MS.gene051827.t1
chr8.2 CDS 6956082 6957608 6956082 ID=cds.MS.gene051827.t1;Parent=MS.gene051827.t1
Gene Sequence

>MS.gene051827

ATGGTTGTGTCTTCTCTGTTTCATCCTTCATTCTCCACCATCGTTTTTAACGCTCCTAAACCCACACATAACCGCCGCACACTCACCGTCGCCACCGCTACCACCAACAACCACTCTCCATCACCGGATCTCGCCACCGACAACATCAAAGACATCGCCCGCCGTCACCCTGCCACCGCCAACAACTTCACCGCCAAATACGTCCCATTCAACTCCACCTTCGACTCCCCTGAATCCTACTCCCTCGACGAAATCGTCTACCGCAGCAACTCCGGCGGCCTTCTCGACGTCCACCATGACATCGAAGCACTCGCGAAGTTCGACGGCGCGTACTGGCGCAACCTCTTCGACTCGCGTGTGGGGAAAACCACTTGGCCTTACGGTTCAGGTGTATGGAGCAAAAAGGAATGGGTCCTACCAGAAATCCACCCTGATGATATCGTTAGCGCTTTTGAAGGTAACTCTAATCTTTTCTGGGCTGAACGTTTTGGCAAACAGTTTGTAGGCATGAACGATCTTTGGGTTAAACACTGTGGTAATAGCCATACTGGAAGCTTCAAAGATCTTGGAATGACTGTGCTTGTTAGTCAGGTAAATCGCCTCCGGAAAATGAACCGTCCGTTGGTCGGAGTTGGTTGTGCTTCCACCGGTGATACATCGGCTGCGCTGTCTGCTTATTGCGCTTCAGCTGGAATTCCTTCGATTGTTTTCCTACCAGCGAATAAAATCTCAACCGCGCAGTTGATTCAACCGGTATCAAATGGTGCATTGGTGTTGAGTATTGATACTGATTTTGATGGTTGTATGAAGCTGATTAGGGAAATTACTTCTGAATTGCCGATATATTTGGCGAATTCCTTGAATAGTTTACGAATTGAAGGACAGAAAACTGCTGCTATTGAGATTTTGCAGCAGTTTGATTGGCAGGTACCTGATTGGGTGATTGTACCTGGTGGAAATTTAGGGAATATTTATGCATTTTACAAAGGTTTCAAAATGTGTAAAGATTTAGGGCTTGTTGATAAGATACCAAGGCTGGTTTGTGCACAAGCTGCAAATGCAAATCCTTTGTATTTGTATTATAAGAATGGTTGGAAAGAGTTTAAAGCGATTAAAGCGGAAACAACTTTTGCTTCTGCTATTCAGATTGGTGATCCTGTTTCAATTGATAGAGCTGTTTATGCATTGAAGAATTCTGATGGGATTGTTGAGGAAGCCAGTGAAGAGGAATTGATGGATGCAATGGCACTTGCTGATTCAACTGGAATGTTTATTTGTCCTCATACTGGTGTGGCTTTAACTGCTTTGATTAAACTCAGGAATAATGGGGTTATTGGAGCCAGTGAGAGGGTTGTGGTTGTTAGTACTGCTCATGGATTGAAATTTGCAGATAGTAAGATTGATTATCATTCTGGGAATATTGCTGGTATTGGTCGTTTTGCTAATCCACCTGTGTCTGTTAAGGCTGATTTTGGTTCTGTTATGGATGTGTTGAAAGATTTTCTGTTGAGTAAAGCACCGAAGTAG
Protein sequence

>MS.gene051827.t1

MVVSSLFHPSFSTIVFNAPKPTHNRRTLTVATATTNNHSPSPDLATDNIKDIARRHPATANNFTAKYVPFNSTFDSPESYSLDEIVYRSNSGGLLDVHHDIEALAKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIHPDDIVSAFEGNSNLFWAERFGKQFVGMNDLWVKHCGNSHTGSFKDLGMTVLVSQVNRLRKMNRPLVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISTAQLIQPVSNGALVLSIDTDFDGCMKLIREITSELPIYLANSLNSLRIEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCKDLGLVDKIPRLVCAQAANANPLYLYYKNGWKEFKAIKAETTFASAIQIGDPVSIDRAVYALKNSDGIVEEASEEELMDAMALADSTGMFICPHTGVALTALIKLRNNGVIGASERVVVVSTAHGLKFADSKIDYHSGNIAGIGRFANPPVSVKADFGSVMDVLKDFLLSKAPK