Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052676.t1 | RHN43796.1 | 91.9 | 595 | 30 | 3 | 3 | 581 | 1 | 593 | 1.40E-295 | 1025 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052676.t1 | Q9ZU97 | 80.2 | 595 | 100 | 4 | 3 | 581 | 1 | 593 | 1.0e-259 | 897.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052676.t1 | A0A396GRL9 | 91.9 | 595 | 30 | 3 | 3 | 581 | 1 | 593 | 9.8e-296 | 1025.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050817 | MS.gene052676 | 0.815089 | 1.11E-51 | -1.69E-46 |
| MS.gene051020 | MS.gene052676 | 0.809996 | 1.44E-50 | -1.69E-46 |
| MS.gene052676 | MS.gene052678 | 0.940536 | 1.97E-100 | -1.69E-46 |
| MS.gene052676 | MS.gene09818 | 0.814123 | 1.81E-51 | -1.69E-46 |
| MS.gene052676 | MS.gene26731 | 0.802239 | 6.17E-49 | -1.69E-46 |
| MS.gene052676 | MS.gene28610 | 0.81024 | 1.27E-50 | -1.69E-46 |
| MS.gene052676 | MS.gene62685 | 0.819905 | 9.08E-53 | -1.69E-46 |
| MS.gene052676 | MS.gene82037 | 0.82458 | 7.47E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 106 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGCGGTATTTATTTAAA+AGG | 0.149069 | 4.1:-1715811 | None:intergenic |
| CCAAGAATGTCTGCATAATA+TGG | 0.252323 | 4.1:-1715291 | None:intergenic |
| CCATATTATGCAGACATTCT+TGG | 0.259591 | 4.1:+1715291 | MS.gene052676:CDS |
| CCGGATAGACAATTCACTTT+TGG | 0.274932 | 4.1:+1717431 | MS.gene052676:CDS |
| GGATAAATTGATTCGTTTGC+TGG | 0.282407 | 4.1:+1718843 | MS.gene052676:CDS |
| TTTATCATGATCACCATTAC+TGG | 0.296139 | 4.1:-1712543 | None:intergenic |
| CGACGCTTTATCGGCAATTC+CGG | 0.317681 | 4.1:+1712331 | MS.gene052676:CDS |
| ATCTGATATACAAGGCAAAA+TGG | 0.336354 | 4.1:-1715316 | None:intergenic |
| AATAACCATGTATATCTTCC+AGG | 0.339284 | 4.1:-1719406 | None:intergenic |
| AACTTATACCTTCCTGTGAT+TGG | 0.339507 | 4.1:-1712617 | None:intergenic |
| CTCTTGAAGTCTGTAGATTA+TGG | 0.356154 | 4.1:+1714983 | MS.gene052676:intron |
| TGAGCAGCACTTTGTACGTT+TGG | 0.380329 | 4.1:-1714021 | None:intergenic |
| GGTAGGATGGATTACTGTAT+TGG | 0.385066 | 4.1:+1714593 | MS.gene052676:CDS |
| CTTTGCTTATCTAAGATTGC+AGG | 0.392824 | 4.1:+1718876 | MS.gene052676:CDS |
| AATAACGAACTCTGCTATCT+TGG | 0.393017 | 4.1:-1713808 | None:intergenic |
| TGCTCACCATCAAGAAAATC+AGG | 0.394167 | 4.1:-1714651 | None:intergenic |
| TGGTCGAATGGCTGAAATAC+TGG | 0.403764 | 4.1:+1715003 | MS.gene052676:CDS |
| TCTTCTGATGTTGCTTCCTC+AGG | 0.409644 | 4.1:+1719444 | MS.gene052676:CDS |
| GATACCACGTAGCGCTTCAT+TGG | 0.415642 | 4.1:-1715481 | None:intergenic |
| TGCTATGGCCGACGCTTTAT+CGG | 0.420175 | 4.1:+1712322 | MS.gene052676:CDS |
| AAACCCAGCATATCAATGTC+TGG | 0.423265 | 4.1:-1714624 | None:intergenic |
| GTTAGCCTCGTAAACTCATC+TGG | 0.432723 | 4.1:-1715043 | None:intergenic |
| CTATCCGGAAATTGTGCATC+AGG | 0.435911 | 4.1:-1717416 | None:intergenic |
| AAGTCTTTGCTTCTTAGCTC+AGG | 0.440076 | 4.1:+1718537 | MS.gene052676:CDS |
| TGTCAAATTCTTAGTACAGC+TGG | 0.448847 | 4.1:+1718822 | MS.gene052676:CDS |
| TGACTGAAAGTGATCAATTC+AGG | 0.451815 | 4.1:+1714227 | MS.gene052676:CDS |
| TGGTTATTTAAAGCACTGTA+TGG | 0.458292 | 4.1:+1719421 | MS.gene052676:CDS |
| CAATCGCTGGTAAATCCTGC+TGG | 0.458314 | 4.1:+1718457 | MS.gene052676:CDS |
| CCAAAAGTGAATTGTCTATC+CGG | 0.463988 | 4.1:-1717431 | None:intergenic |
| CACCTTGGCAGCTGATGCTA+TGG | 0.467831 | 4.1:+1712307 | None:intergenic |
| TAGTACGGTGATTCAATCTC+TGG | 0.471050 | 4.1:+1718783 | MS.gene052676:CDS |
| TGTCCCAGACATTGATATGC+TGG | 0.472510 | 4.1:+1714620 | MS.gene052676:CDS |
| AGTGCGGTATTTATTTAAAA+GGG | 0.474391 | 4.1:-1715810 | None:intergenic |
| TCAGGCAGTTATCTAGTGAA+TGG | 0.479515 | 4.1:+1715750 | MS.gene052676:intron |
| TGCATAAGGCATCAAAGATC+TGG | 0.484889 | 4.1:-1713978 | None:intergenic |
| ATCTGCAGAAAGCTTGCATA+AGG | 0.490478 | 4.1:-1713992 | None:intergenic |
| AACGAGTTTGTCAAACTTGG+CGG | 0.490637 | 4.1:+1715255 | MS.gene052676:CDS |
| TCTACATCAAATGCTTCAGC+TGG | 0.491088 | 4.1:-1715513 | None:intergenic |
| GATTCGTTTGCTGGAAACTC+CGG | 0.495786 | 4.1:+1718852 | MS.gene052676:CDS |
| AATCCATCCTCTCTATTGCC+AGG | 0.496521 | 4.1:+1715535 | MS.gene052676:CDS |
| GCGCAAGAATGCTGCTCTTG+AGG | 0.500462 | 4.1:+1712394 | MS.gene052676:CDS |
| GTAGCGCTTCATTGGTTTCA+CGG | 0.503711 | 4.1:-1715473 | None:intergenic |
| TAGCGCTTCATTGGTTTCAC+GGG | 0.507753 | 4.1:-1715472 | None:intergenic |
| CCTTCACAAGCCGATCTAAG+AGG | 0.508826 | 4.1:-1714044 | None:intergenic |
| AGATTACGAAGAACGGCTGC+CGG | 0.512307 | 4.1:-1712350 | None:intergenic |
| TGAAGCGCTACGTGGTATCA+AGG | 0.512554 | 4.1:+1715485 | MS.gene052676:CDS |
| TCTTCTTTATCTGATATACA+AGG | 0.513178 | 4.1:-1715324 | None:intergenic |
| CAAAGACTTATAATCGCCAT+TGG | 0.516247 | 4.1:-1718523 | None:intergenic |
| GATCTGAAAGTGCCTTGAGC+AGG | 0.517459 | 4.1:-1716658 | None:intergenic |
| ATGTTTCTTTGTATGCCTCA+TGG | 0.518384 | 4.1:+1718491 | MS.gene052676:CDS |
| CCTCCTGGCAATAGAGAGGA+TGG | 0.518633 | 4.1:-1715538 | None:intergenic |
| CAGGCAGTTATCTAGTGAAT+GGG | 0.520703 | 4.1:+1715751 | MS.gene052676:intron |
| CTCGAATTGAAGCTTTGCAC+TGG | 0.521202 | 4.1:+1715780 | MS.gene052676:CDS |
| GCGGGGCTTTAATAATCCGC+AGG | 0.531158 | 4.1:+1717574 | MS.gene052676:CDS |
| GTCCCAGACATTGATATGCT+GGG | 0.533041 | 4.1:+1714621 | MS.gene052676:CDS |
| ACATATAAATCCTTCCCAGC+AGG | 0.533816 | 4.1:-1718472 | None:intergenic |
| ATAAACGAGTTTGTCAAACT+TGG | 0.535432 | 4.1:+1715252 | MS.gene052676:CDS |
| CGAGGCTAACATCTATCACA+TGG | 0.544832 | 4.1:+1715056 | MS.gene052676:CDS |
| GACTTACCAACATGCTAGTA+CGG | 0.553041 | 4.1:+1718768 | MS.gene052676:intron |
| GGCTGAAATACTGGTACAGA+GGG | 0.558319 | 4.1:+1715012 | MS.gene052676:CDS |
| TCGGAGAAAGAATTTAGCAC+AGG | 0.559335 | 4.1:-1713785 | None:intergenic |
| CGGAGAAAGAATTTAGCACA+GGG | 0.562816 | 4.1:-1713784 | None:intergenic |
| TATAAGGCTACCTCATCAGA+TGG | 0.564243 | 4.1:-1716680 | None:intergenic |
| GCTGGTAAATCCTGCTGGGA+AGG | 0.564523 | 4.1:+1718462 | MS.gene052676:CDS |
| AACCCAGCATATCAATGTCT+GGG | 0.566359 | 4.1:-1714623 | None:intergenic |
| GAAATACTGGTACAGAGGGC+AGG | 0.567212 | 4.1:+1715016 | MS.gene052676:CDS |
| ACGAACTCTGCTATCTTGGT+CGG | 0.573813 | 4.1:-1713804 | None:intergenic |
| AAGAATTTATACCATTGCTG+AGG | 0.580312 | 4.1:+1714529 | MS.gene052676:CDS |
| GTGAAGCAATTAGCCAGTAA+TGG | 0.580455 | 4.1:+1712530 | MS.gene052676:CDS |
| TATTCACACATGCCAATCAC+AGG | 0.581766 | 4.1:+1712605 | MS.gene052676:CDS |
| ATCAGCAAGATTACGAAGAA+CGG | 0.585392 | 4.1:-1712357 | None:intergenic |
| AGAATTTATACCATTGCTGA+GGG | 0.587882 | 4.1:+1714530 | MS.gene052676:CDS |
| AGCATTATATAACATAGCTA+AGG | 0.590894 | 4.1:+1713841 | MS.gene052676:CDS |
| GTGCTGCTCACCTCTTAGAT+CGG | 0.592833 | 4.1:+1714034 | MS.gene052676:CDS |
| AATCGCTGGTAAATCCTGCT+GGG | 0.594476 | 4.1:+1718458 | MS.gene052676:CDS |
| GTCTGTAGATTATGGTCGAA+TGG | 0.594824 | 4.1:+1714991 | MS.gene052676:intron |
| CTCATGGTGTCATTCTCCAA+TGG | 0.600308 | 4.1:+1718507 | MS.gene052676:CDS |
| TTCATGCGCTCCCTCAGCAA+TGG | 0.600314 | 4.1:-1714540 | None:intergenic |
| CCTCTTAGATCGGCTTGTGA+AGG | 0.602157 | 4.1:+1714044 | MS.gene052676:CDS |
| CATATTCGACACCCTGCTCA+AGG | 0.604726 | 4.1:+1716646 | MS.gene052676:CDS |
| CTGCATAATATGGAACAAGC+TGG | 0.606209 | 4.1:-1715281 | None:intergenic |
| TGGAACCTGGAAGATATACA+TGG | 0.606249 | 4.1:+1719401 | MS.gene052676:CDS |
| TGGCTGAAATACTGGTACAG+AGG | 0.607464 | 4.1:+1715011 | MS.gene052676:CDS |
| GATTCAATCTCTGGCAGAGG+AGG | 0.607465 | 4.1:+1718792 | MS.gene052676:CDS |
| GGTGATTCAATCTCTGGCAG+AGG | 0.609355 | 4.1:+1718789 | MS.gene052676:CDS |
| AATCTTAGATAAGCAAAGAC+CGG | 0.610998 | 4.1:-1718871 | None:intergenic |
| GAATCACCGTACTAGCATGT+TGG | 0.633613 | 4.1:-1718774 | None:intergenic |
| GCAGTTATCTAGTGAATGGG+AGG | 0.636635 | 4.1:+1715754 | MS.gene052676:CDS |
| TATACCTCCTGGCAATAGAG+AGG | 0.639677 | 4.1:-1715542 | None:intergenic |
| TGTATTATATTCTAGGCGCG+GGG | 0.650081 | 4.1:+1717557 | MS.gene052676:intron |
| CACACATGCCAATCACAGGA+AGG | 0.652455 | 4.1:+1712609 | MS.gene052676:CDS |
| CGGAATTGCCGATAAAGCGT+CGG | 0.658159 | 4.1:-1712330 | None:intergenic |
| ACTTTCAGATCCATCTGATG+AGG | 0.663441 | 4.1:+1716670 | MS.gene052676:CDS |
| ATCTGAAAGTGCCTTGAGCA+GGG | 0.667373 | 4.1:-1716657 | None:intergenic |
| ACAAGCTGACGAAAGTGCTG+AGG | 0.681038 | 4.1:-1717387 | None:intergenic |
| TGGTGATCATGATAAAATTG+CGG | 0.681448 | 4.1:+1712550 | MS.gene052676:CDS |
| TTTAAATAAATACCGCACTG+AGG | 0.681709 | 4.1:+1715814 | MS.gene052676:CDS |
| GAGAAAGAATTTAGCACAGG+GGG | 0.683204 | 4.1:-1713782 | None:intergenic |
| GAAACCAATGAAGCGCTACG+TGG | 0.695293 | 4.1:+1715477 | MS.gene052676:CDS |
| TCAGAAGCACACAAAGCCTG+CGG | 0.698794 | 4.1:-1717590 | None:intergenic |
| AAAATTTAGTTACCTCAGTG+CGG | 0.699542 | 4.1:-1715826 | None:intergenic |
| GTTCTCCAGATGAGTTTACG+AGG | 0.707570 | 4.1:+1715038 | MS.gene052676:CDS |
| CATTGGAGAATGACACCATG+AGG | 0.708850 | 4.1:-1718506 | None:intergenic |
| GAGGCTAACATCTATCACAT+GGG | 0.709955 | 4.1:+1715057 | MS.gene052676:CDS |
| CCATCCTCTCTATTGCCAGG+AGG | 0.718696 | 4.1:+1715538 | MS.gene052676:CDS |
| GGAGAAAGAATTTAGCACAG+GGG | 0.776546 | 4.1:-1713783 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 1712318 | 1721131 | 1712318 | ID=MS.gene052676 |
| chr4.1 | mRNA | 1712318 | 1721131 | 1712318 | ID=MS.gene052676.t1;Parent=MS.gene052676 |
| chr4.1 | exon | 1712318 | 1712415 | 1712318 | ID=MS.gene052676.t1.exon1;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1712318 | 1712415 | 1712318 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1712518 | 1712636 | 1712518 | ID=MS.gene052676.t1.exon2;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1712518 | 1712636 | 1712518 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1713751 | 1713862 | 1713751 | ID=MS.gene052676.t1.exon3;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1713751 | 1713862 | 1713751 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1713946 | 1714065 | 1713946 | ID=MS.gene052676.t1.exon4;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1713946 | 1714065 | 1713946 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1714220 | 1714248 | 1714220 | ID=MS.gene052676.t1.exon5;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1714220 | 1714248 | 1714220 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1714524 | 1714666 | 1714524 | ID=MS.gene052676.t1.exon6;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1714524 | 1714666 | 1714524 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1714763 | 1714857 | 1714763 | ID=MS.gene052676.t1.exon7;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1714763 | 1714857 | 1714763 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1714992 | 1715078 | 1714992 | ID=MS.gene052676.t1.exon8;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1714992 | 1715078 | 1714992 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1715252 | 1715359 | 1715252 | ID=MS.gene052676.t1.exon9;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1715252 | 1715359 | 1715252 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1715468 | 1715559 | 1715468 | ID=MS.gene052676.t1.exon10;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1715468 | 1715559 | 1715468 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1715754 | 1715835 | 1715754 | ID=MS.gene052676.t1.exon11;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1715754 | 1715835 | 1715754 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1716626 | 1716691 | 1716626 | ID=MS.gene052676.t1.exon12;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1716626 | 1716691 | 1716626 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1717342 | 1717457 | 1717342 | ID=MS.gene052676.t1.exon13;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1717342 | 1717457 | 1717342 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1717572 | 1717630 | 1717572 | ID=MS.gene052676.t1.exon14;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1717572 | 1717630 | 1717572 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1718338 | 1718558 | 1718338 | ID=MS.gene052676.t1.exon15;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1718338 | 1718558 | 1718338 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1718769 | 1718897 | 1718769 | ID=MS.gene052676.t1.exon16;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1718769 | 1718897 | 1718769 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1719397 | 1719465 | 1719397 | ID=MS.gene052676.t1.exon17;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1719397 | 1719465 | 1719397 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| chr4.1 | exon | 1721114 | 1721131 | 1721114 | ID=MS.gene052676.t1.exon18;Parent=MS.gene052676.t1 |
| chr4.1 | CDS | 1721114 | 1721131 | 1721114 | ID=cds.MS.gene052676.t1;Parent=MS.gene052676.t1 |
| Gene Sequence |
| Protein sequence |
