Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052865.t1 | XP_013445629.1 | 93.3 | 240 | 13 | 1 | 1 | 240 | 1 | 237 | 2.20E-127 | 464.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052865.t1 | Q6R567 | 46.2 | 247 | 114 | 7 | 1 | 237 | 5 | 242 | 1.5e-52 | 207.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052865.t1 | A0A072U1G6 | 93.3 | 240 | 13 | 1 | 1 | 240 | 1 | 237 | 1.6e-127 | 464.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052865 | MS.gene53923 | 0.948351 | 1.12E-106 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene032302 | MS.gene052865 | PPI |
| MS.gene36072 | MS.gene052865 | PPI |
| MS.gene34211 | MS.gene052865 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052865.t1 | MTR_8g465910 | 92.946 | 241 | 14 | 1 | 1 | 241 | 1 | 238 | 3.40e-170 | 469 |
| MS.gene052865.t1 | MTR_2g090295 | 54.878 | 246 | 89 | 7 | 1 | 235 | 1 | 235 | 2.27e-91 | 269 |
| MS.gene052865.t1 | MTR_2g090330 | 60.000 | 110 | 40 | 1 | 36 | 145 | 25 | 130 | 3.86e-45 | 149 |
| MS.gene052865.t1 | MTR_2g090330 | 60.000 | 110 | 40 | 1 | 36 | 145 | 22 | 127 | 5.61e-45 | 148 |
| MS.gene052865.t1 | MTR_2g013550 | 41.589 | 214 | 99 | 7 | 36 | 233 | 19 | 222 | 4.50e-41 | 140 |
| MS.gene052865.t1 | MTR_2g090325 | 51.370 | 146 | 44 | 5 | 36 | 168 | 10 | 141 | 1.46e-40 | 138 |
| MS.gene052865.t1 | MTR_4g088875 | 38.462 | 221 | 111 | 8 | 24 | 233 | 11 | 217 | 3.06e-38 | 133 |
| MS.gene052865.t1 | MTR_4g088870 | 45.536 | 112 | 55 | 3 | 24 | 131 | 11 | 120 | 9.41e-31 | 113 |
| MS.gene052865.t1 | MTR_1g021895 | 48.421 | 95 | 40 | 2 | 41 | 135 | 27 | 112 | 1.65e-27 | 105 |
| MS.gene052865.t1 | MTR_5g019300 | 48.913 | 92 | 38 | 2 | 44 | 135 | 35 | 117 | 5.13e-27 | 104 |
| MS.gene052865.t1 | MTR_1g054260 | 38.525 | 122 | 71 | 3 | 22 | 139 | 62 | 183 | 1.78e-23 | 94.4 |
| MS.gene052865.t1 | MTR_4g029440 | 46.739 | 92 | 41 | 2 | 44 | 135 | 126 | 209 | 2.80e-23 | 97.4 |
| MS.gene052865.t1 | MTR_4g029440 | 46.739 | 92 | 41 | 2 | 44 | 135 | 132 | 215 | 2.81e-23 | 97.4 |
| MS.gene052865.t1 | MTR_1g054465 | 38.835 | 103 | 61 | 2 | 33 | 133 | 43 | 145 | 2.87e-23 | 93.6 |
| MS.gene052865.t1 | MTR_7g093390 | 34.722 | 144 | 70 | 5 | 5 | 135 | 118 | 250 | 1.52e-22 | 95.9 |
| MS.gene052865.t1 | MTR_1g054280 | 40.909 | 110 | 51 | 3 | 42 | 151 | 74 | 169 | 1.86e-22 | 92.8 |
| MS.gene052865.t1 | MTR_1g054210 | 44.565 | 92 | 43 | 3 | 44 | 135 | 122 | 205 | 4.67e-22 | 91.3 |
| MS.gene052865.t1 | MTR_2g061230 | 38.889 | 108 | 55 | 3 | 28 | 135 | 94 | 190 | 1.40e-19 | 84.7 |
| MS.gene052865.t1 | MTR_1g015390 | 38.667 | 75 | 34 | 2 | 34 | 107 | 353 | 416 | 1.88e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052865.t1 | AT4G03510 | 42.387 | 243 | 126 | 6 | 1 | 235 | 1 | 237 | 5.93e-57 | 182 |
| MS.gene052865.t1 | AT4G03510 | 42.387 | 243 | 126 | 6 | 1 | 235 | 1 | 237 | 5.93e-57 | 182 |
| MS.gene052865.t1 | AT4G03510 | 42.387 | 243 | 126 | 6 | 1 | 235 | 1 | 237 | 5.93e-57 | 182 |
| MS.gene052865.t1 | AT4G03510 | 42.387 | 243 | 126 | 6 | 1 | 235 | 1 | 237 | 5.93e-57 | 182 |
| MS.gene052865.t1 | AT4G27470 | 37.555 | 229 | 109 | 8 | 18 | 233 | 22 | 229 | 7.98e-39 | 135 |
| MS.gene052865.t1 | AT4G28270 | 37.688 | 199 | 85 | 6 | 39 | 232 | 14 | 178 | 1.32e-36 | 128 |
| MS.gene052865.t1 | AT1G19310 | 51.087 | 92 | 36 | 2 | 44 | 135 | 21 | 103 | 1.82e-27 | 105 |
| MS.gene052865.t1 | AT1G74990 | 37.903 | 124 | 64 | 3 | 29 | 152 | 2 | 112 | 2.64e-27 | 102 |
| MS.gene052865.t1 | AT2G23780 | 45.098 | 102 | 46 | 3 | 35 | 135 | 16 | 108 | 2.20e-25 | 100 |
| MS.gene052865.t1 | AT2G23780 | 45.098 | 102 | 46 | 3 | 35 | 135 | 16 | 108 | 2.20e-25 | 100 |
| MS.gene052865.t1 | AT2G23780 | 45.098 | 102 | 46 | 3 | 35 | 135 | 16 | 108 | 2.20e-25 | 100 |
| MS.gene052865.t1 | AT3G58030 | 42.593 | 108 | 47 | 3 | 31 | 135 | 121 | 216 | 8.19e-23 | 96.3 |
| MS.gene052865.t1 | AT3G58030 | 42.593 | 108 | 47 | 3 | 31 | 135 | 121 | 216 | 8.19e-23 | 96.3 |
| MS.gene052865.t1 | AT3G58030 | 42.593 | 108 | 47 | 3 | 31 | 135 | 121 | 216 | 8.19e-23 | 96.3 |
| MS.gene052865.t1 | AT3G58030 | 42.593 | 108 | 47 | 3 | 31 | 135 | 121 | 216 | 8.19e-23 | 96.3 |
| MS.gene052865.t1 | AT2G42030 | 40.404 | 99 | 45 | 3 | 44 | 141 | 139 | 224 | 4.68e-21 | 91.3 |
| MS.gene052865.t1 | AT2G44410 | 32.609 | 138 | 79 | 3 | 4 | 135 | 77 | 206 | 4.30e-19 | 85.9 |
| MS.gene052865.t1 | AT2G26350 | 42.169 | 83 | 31 | 4 | 25 | 107 | 312 | 377 | 2.10e-11 | 63.2 |
| MS.gene052865.t1 | AT2G26350 | 42.169 | 83 | 31 | 4 | 25 | 107 | 241 | 306 | 2.70e-11 | 62.8 |
Find 0 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACTGAATATAATCTATACA+AGG | - | 31140:12378-12397 | None:intergenic | 20.0% |
| !!! | ATGTATAAATGTTTTCTATC+TGG | - | 31140:12182-12201 | None:intergenic | 20.0% |
| !!! | CTAATTCTTATAGTTTTCAA+GGG | + | 31140:12205-12224 | MS.gene052865:CDS | 20.0% |
| ! | ATCTTTGGTGAAATGATATA+TGG | + | 31140:12141-12160 | MS.gene052865:CDS | 25.0% |
| ! | CCTTGAAAACTATAAGAATT+AGG | - | 31140:12207-12226 | None:intergenic | 25.0% |
| !! | CCTAATTCTTATAGTTTTCA+AGG | + | 31140:12204-12223 | MS.gene052865:CDS | 25.0% |
| !!! | TGTTGATAACTTTGATAAGT+AGG | - | 31140:12027-12046 | None:intergenic | 25.0% |
| ATGATATATGGAAGGATGTT+TGG | + | 31140:12153-12172 | MS.gene052865:CDS | 30.0% | |
| ATTAAATGTCTTCTCATCCT+TGG | - | 31140:12240-12259 | None:intergenic | 30.0% | |
| GTTGTAGCATTAAATGATCT+TGG | - | 31140:11994-12013 | None:intergenic | 30.0% | |
| TTGGTGAAATGATATATGGA+AGG | + | 31140:12145-12164 | MS.gene052865:CDS | 30.0% | |
| ! | ATAAGCCATCTTTGAAAAAC+TGG | + | 31140:11647-11666 | MS.gene052865:CDS | 30.0% |
| !! | AAAGGTATCAATTTGAGAGA+CGG | - | 31140:11623-11642 | None:intergenic | 30.0% |
| !! | GTTTTCCAGTTTTTCAAAGA+TGG | - | 31140:11655-11674 | None:intergenic | 30.0% |
| AAATGGCTTGACATACAAAG+TGG | + | 31140:11805-11824 | MS.gene052865:CDS | 35.0% | |
| ACAAAACTAAGCTGCAAAAG+AGG | - | 31140:12299-12318 | None:intergenic | 35.0% | |
| ATCAGAATCTGCAATCACAT+CGG | - | 31140:11683-11702 | None:intergenic | 35.0% | |
| CAAAGATGGCTTATCTTCAA+AGG | - | 31140:11641-11660 | None:intergenic | 35.0% | |
| GAACCACTTGTACTAAACAT+CGG | - | 31140:12099-12118 | None:intergenic | 35.0% | |
| GATAACACATACGGAATCTT+TGG | + | 31140:12126-12145 | MS.gene052865:CDS | 35.0% | |
| GATGTTTAGTACAAGTGGTT+CGG | + | 31140:12098-12117 | MS.gene052865:CDS | 35.0% | |
| TCAAGCCATTTGTAAATGCA+AGG | - | 31140:11796-11815 | None:intergenic | 35.0% | |
| TCTGATAGAAATGCATCTAG+TGG | + | 31140:11697-11716 | MS.gene052865:CDS | 35.0% | |
| TCTTCTCATCCTTGGATTAT+TGG | - | 31140:12232-12251 | None:intergenic | 35.0% | |
| TTGTACTAAACATCGGTGAA+GGG | - | 31140:12092-12111 | None:intergenic | 35.0% | |
| ATCAACATCCTCATCATCCT+CGG | + | 31140:12040-12059 | MS.gene052865:CDS | 40.0% | |
| CTTGTACTAAACATCGGTGA+AGG | - | 31140:12093-12112 | None:intergenic | 40.0% | |
| TCACCGATGTTTAGTACAAG+TGG | + | 31140:12093-12112 | MS.gene052865:CDS | 40.0% | |
| TCCTCGCTAGTTCCATTATA+TGG | + | 31140:11892-11911 | MS.gene052865:CDS | 40.0% | |
| TCGGCACTAGATAACACATA+CGG | + | 31140:12117-12136 | MS.gene052865:CDS | 40.0% | |
| TGACCACAAAAAGTGACTAC+CGG | - | 31140:11760-11779 | None:intergenic | 40.0% | |
| TTAAATGATCTTGGACCCGT+AGG | - | 31140:11985-12004 | None:intergenic | 40.0% | |
| TTCAAGGGACCAATAATCCA+AGG | + | 31140:12220-12239 | MS.gene052865:CDS | 40.0% | |
| TTTGTGGTCATCTTTACTGC+TGG | + | 31140:11770-11789 | MS.gene052865:CDS | 40.0% | |
| ! | ATCGTCCATGTACTGATCTA+AGG | - | 31140:11599-11618 | None:intergenic | 40.0% |
| ! | GTGAGTTCTGATTTGCATAC+CGG | - | 31140:11868-11887 | None:intergenic | 40.0% |
| AGAATCTGCAATCACATCGG+TGG | - | 31140:11680-11699 | None:intergenic | 45.0% | |
| CAAACCATGACTGTGTCTGA+AGG | + | 31140:11922-11941 | MS.gene052865:CDS | 45.0% | |
| CTAGTTCCATTATATGGCCG+TGG | + | 31140:11898-11917 | MS.gene052865:CDS | 45.0% | |
| CTTCAGACACAGTCATGGTT+TGG | - | 31140:11924-11943 | None:intergenic | 45.0% | |
| CTTTGACTGTAACATCTGCC+TGG | + | 31140:11720-11739 | MS.gene052865:CDS | 45.0% | |
| GACCACAAAAAGTGACTACC+GGG | - | 31140:11759-11778 | None:intergenic | 45.0% | |
| GATTTGCATACCGGACATTG+TGG | - | 31140:11859-11878 | None:intergenic | 45.0% | |
| TTGAATTGCCGAGGATGATG+AGG | - | 31140:12051-12070 | None:intergenic | 45.0% | |
| ! | GCCATATAATGGAACTAGCG+AGG | - | 31140:11896-11915 | None:intergenic | 45.0% |
| ! | TGGCTTCTGCTTCTCGTTTT+CGG | - | 31140:11839-11858 | None:intergenic | 45.0% |
| !! | GCTGGCCTTGCATTTACAAA+TGG | + | 31140:11788-11807 | MS.gene052865:CDS | 45.0% |
| !! | GGAATTGAGTTGAATTGCCG+AGG | - | 31140:12060-12079 | None:intergenic | 45.0% |
| AAGGCAAGGCTCAGCAAGTA+GGG | + | 31140:11941-11960 | MS.gene052865:CDS | 50.0% | |
| ATCGGTGAAGGGTAACTGCT+CGG | - | 31140:12081-12100 | None:intergenic | 50.0% | |
| ATCTGCAATCACATCGGTGG+AGG | - | 31140:11677-11696 | None:intergenic | 50.0% | |
| CATGACTGTGTCTGAAGGCA+AGG | + | 31140:11927-11946 | MS.gene052865:CDS | 50.0% | |
| CTTGCCTTCAGACACAGTCA+TGG | - | 31140:11929-11948 | None:intergenic | 50.0% | |
| GAAGCAGAAGCCACAATGTC+CGG | + | 31140:11846-11865 | MS.gene052865:CDS | 50.0% | |
| GTTTGGCCACGGCCATATAA+TGG | - | 31140:11907-11926 | None:intergenic | 50.0% | |
| ! | ACACAGTCATGGTTTGGCCA+CGG | - | 31140:11918-11937 | None:intergenic | 50.0% |
| !!! | GACCCGGTAGTCACTTTTTG+TGG | + | 31140:11754-11773 | MS.gene052865:CDS | 50.0% |
| CCTGGATTGTGTGCAAGACC+CGG | + | 31140:11738-11757 | MS.gene052865:CDS | 55.0% | |
| GAAGGCAAGGCTCAGCAAGT+AGG | + | 31140:11940-11959 | MS.gene052865:CDS | 55.0% | |
| TGTCGTGCCTCGAAGACCTA+CGG | + | 31140:11966-11985 | MS.gene052865:CDS | 55.0% | |
| CCGGGTCTTGCACACAATCC+AGG | - | 31140:11741-11760 | None:intergenic | 60.0% | |
| CTTGGACCCGTAGGTCTTCG+AGG | - | 31140:11976-11995 | None:intergenic | 60.0% | |
| GTCGTGCCTCGAAGACCTAC+GGG | + | 31140:11967-11986 | MS.gene052865:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 31140 | gene | 11592 | 12413 | 11592 | ID=MS.gene052865 |
| 31140 | mRNA | 11592 | 12413 | 11592 | ID=MS.gene052865.t1;Parent=MS.gene052865 |
| 31140 | exon | 11592 | 12288 | 11592 | ID=MS.gene052865.t1.exon1;Parent=MS.gene052865.t1 |
| 31140 | CDS | 11592 | 12288 | 11592 | ID=cds.MS.gene052865.t1;Parent=MS.gene052865.t1 |
| 31140 | exon | 12385 | 12413 | 12385 | ID=MS.gene052865.t1.exon2;Parent=MS.gene052865.t1 |
| 31140 | CDS | 12385 | 12413 | 12385 | ID=cds.MS.gene052865.t1;Parent=MS.gene052865.t1 |
| Gene Sequence |
| Protein sequence |
