AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032302


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032302.t1 MTR_8g465910 44.269 253 102 11 10 250 19 244 1.29e-61 194
MS.gene032302.t1 MTR_2g090295 40.496 242 107 8 10 238 18 235 2.88e-51 167
MS.gene032302.t1 MTR_4g088875 38.793 232 116 7 11 236 6 217 8.69e-45 150
MS.gene032302.t1 MTR_4g088870 35.772 246 100 6 11 250 6 199 1.45e-42 144
MS.gene032302.t1 MTR_2g013550 42.466 219 114 7 21 236 13 222 2.26e-42 144
MS.gene032302.t1 MTR_2g090330 60.674 89 33 1 32 120 30 116 3.17e-33 119
MS.gene032302.t1 MTR_2g090330 60.674 89 33 1 32 120 27 113 3.97e-33 118
MS.gene032302.t1 MTR_2g090325 48.760 121 39 4 35 145 18 125 7.84e-30 110
MS.gene032302.t1 MTR_1g021895 55.814 86 31 2 35 120 30 108 4.90e-29 109
MS.gene032302.t1 MTR_5g019300 51.648 91 37 2 30 120 30 113 2.64e-27 105
MS.gene032302.t1 MTR_1g054280 41.593 113 60 2 8 120 49 155 1.06e-23 96.7
MS.gene032302.t1 MTR_7g093390 43.810 105 48 3 16 120 153 246 2.38e-22 95.5
MS.gene032302.t1 MTR_1g054210 35.556 135 77 4 8 139 93 220 5.33e-21 88.6
MS.gene032302.t1 MTR_4g029440 38.776 98 54 1 23 120 120 211 6.48e-21 91.3
MS.gene032302.t1 MTR_4g029440 38.776 98 54 1 23 120 114 205 6.57e-21 91.3
MS.gene032302.t1 MTR_1g054260 37.500 128 66 4 19 137 65 187 1.07e-20 87.0
MS.gene032302.t1 MTR_1g054465 35.398 113 70 2 11 120 31 143 1.65e-19 84.0
MS.gene032302.t1 MTR_2g061230 40.909 88 46 1 33 120 105 186 2.46e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032302.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene032302.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene032302.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene032302.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene032302.t1 AT4G27470 49.123 228 90 10 15 236 22 229 2.47e-53 172
MS.gene032302.t1 AT4G28270 35.685 241 94 8 19 250 5 193 6.89e-40 137
MS.gene032302.t1 AT2G23780 34.733 262 111 11 9 248 3 226 2.67e-31 115
MS.gene032302.t1 AT2G23780 34.733 262 111 11 9 248 3 226 2.67e-31 115
MS.gene032302.t1 AT2G23780 34.733 262 111 11 9 248 3 226 2.67e-31 115
MS.gene032302.t1 AT1G19310 58.140 86 29 2 35 120 21 99 1.07e-29 111
MS.gene032302.t1 AT1G74990 53.061 98 36 3 26 120 5 95 2.13e-28 105
MS.gene032302.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene032302.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene032302.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene032302.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene032302.t1 AT2G42030 44.444 99 41 3 35 131 139 225 1.85e-20 90.1
MS.gene032302.t1 AT2G44410 40.000 90 40 2 35 120 123 202 6.14e-18 82.8

Find 66 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACCAACCTGTAGTTTAT+TGG 0.128251 8.2:-47814270 None:intergenic
ATTTCCTGCCTTCTCATTCT+AGG 0.172452 8.2:-47814605 None:intergenic
CACTACATCCCTCACCTTTA+TGG 0.179900 8.2:+47814425 MS.gene032302:CDS
AACATAGCATTTAAGTTATA+AGG 0.203842 8.2:-47814314 None:intergenic
ATCCTGTGATGGGTTTGTTT+GGG 0.307382 8.2:+47814507 MS.gene032302:CDS
ACTTCATTAGTCCCACTTTA+CGG 0.312481 8.2:+47814218 MS.gene032302:CDS
GATGGGTTTGTTTGGGGAGA+TGG 0.320135 8.2:+47814514 MS.gene032302:CDS
AAAGTGGGACTAATGAAGTA+TGG 0.333058 8.2:-47814214 None:intergenic
AATCCTGTGATGGGTTTGTT+TGG 0.347652 8.2:+47814506 MS.gene032302:CDS
TCAATGGAATCTACATGCTT+TGG 0.371719 8.2:+47813948 None:intergenic
AACCTGTAGTTTATTGGATT+CGG 0.384366 8.2:-47814264 None:intergenic
TCCTCGTGATGTAGATTTGA+TGG 0.387604 8.2:-47814350 None:intergenic
CCAAAGCTCTTCTGTTGAAC+TGG 0.390019 8.2:+47814151 MS.gene032302:CDS
GCCAATGGGTTACATTATCT+TGG 0.409333 8.2:+47814458 MS.gene032302:CDS
CCAGTTCAACAGAAGAGCTT+TGG 0.409537 8.2:-47814151 None:intergenic
ATATGCATGGCCAGCAATAC+AGG 0.414858 8.2:-47814112 None:intergenic
TCTGTGGTCACCTGTATTGC+TGG 0.419618 8.2:+47814102 MS.gene032302:CDS
CTTTGATTGCAATATTTGCT+TGG 0.432126 8.2:+47814052 MS.gene032302:CDS
CTGCCTTCTCATTCTAGGAC+TGG 0.437502 8.2:-47814600 None:intergenic
GGACTGGTACTTCCGTTATG+AGG 0.441104 8.2:-47814584 None:intergenic
TGGCACTATGACCTCCATAA+AGG 0.443573 8.2:-47814439 None:intergenic
GAGATCACGGCTTTACAAAC+CGG 0.457250 8.2:-47814191 None:intergenic
GAATTGGTCGCGATTGTGAC+TGG 0.479790 8.2:-47814394 None:intergenic
TAGTTTCGTCAATCCTGTGA+TGG 0.480852 8.2:+47814496 MS.gene032302:CDS
ATGGTGTCGACAAGGATGTT+TGG 0.510014 8.2:+47814533 MS.gene032302:CDS
GATTTCCCTCGGCCGTAAAG+TGG 0.513776 8.2:-47814230 None:intergenic
TATGGAGGTCATAGTGCCAA+TGG 0.516603 8.2:+47814443 MS.gene032302:CDS
AACCGAATCCAATAAACTAC+AGG 0.526244 8.2:+47814262 MS.gene032302:CDS
AGTTTCGTCAATCCTGTGAT+GGG 0.527399 8.2:+47814497 MS.gene032302:CDS
GATTGTGACTGGAAATAGTT+CGG 0.527943 8.2:-47814383 None:intergenic
GATTGTTGTATCTTCTGTAG+TGG 0.527970 8.2:-47814009 None:intergenic
ATGGAGGTCATAGTGCCAAT+GGG 0.535195 8.2:+47814444 MS.gene032302:CDS
GCATTTAAGTTATAAGGAGG+TGG 0.545819 8.2:-47814308 None:intergenic
TTTCTGAATTAGATTTCCCT+CGG 0.545966 8.2:-47814241 None:intergenic
TTAGTCCCACTTTACGGCCG+AGG 0.552806 8.2:+47814224 MS.gene032302:CDS
GCTGGCCATGCATATATAAG+TGG 0.555073 8.2:+47814120 MS.gene032302:CDS
AAGTGGGACTAATGAAGTAT+GGG 0.558591 8.2:-47814213 None:intergenic
TCCTGTGATGGGTTTGTTTG+GGG 0.558787 8.2:+47814508 MS.gene032302:CDS
TTGTTTGCGTACCCTCATAA+CGG 0.562898 8.2:+47814572 MS.gene032302:CDS
TCCCCAAACAAACCCATCAC+AGG 0.565091 8.2:-47814509 None:intergenic
GCTTTACAAACCGGGCATGT+TGG 0.566623 8.2:-47814182 None:intergenic
ATGTAGTGCTGATAATGAAT+TGG 0.567201 8.2:-47814410 None:intergenic
AATGGGTTACATTATCTTGG+TGG 0.568227 8.2:+47814461 MS.gene032302:CDS
TAGTCCCACTTTACGGCCGA+GGG 0.568488 8.2:+47814225 MS.gene032302:CDS
ATTTCCCTCGGCCGTAAAGT+GGG 0.571345 8.2:-47814229 None:intergenic
GGATACGCAGCCAACATGCC+CGG 0.580139 8.2:+47814172 MS.gene032302:CDS
GACTGGTACTTCCGTTATGA+GGG 0.589526 8.2:-47814583 None:intergenic
GAATCCAATAAACTACAGGT+TGG 0.591477 8.2:+47814266 MS.gene032302:CDS
TAATGAAGTATGGGAGATCA+CGG 0.595278 8.2:-47814204 None:intergenic
CCTCGTGATGTAGATTTGAT+GGG 0.597079 8.2:-47814349 None:intergenic
TTAAGCCACTTATATATGCA+TGG 0.597413 8.2:-47814125 None:intergenic
CAGTCCTAGAATGAGAAGGC+AGG 0.602401 8.2:+47814601 MS.gene032302:CDS
ACCAAGATAATGTAACCCAT+TGG 0.617703 8.2:-47814459 None:intergenic
CCCATCAAATCTACATCACG+AGG 0.619297 8.2:+47814349 MS.gene032302:CDS
ATAGCATTTAAGTTATAAGG+AGG 0.631590 8.2:-47814311 None:intergenic
TATGACCTCCATAAAGGTGA+GGG 0.631991 8.2:-47814433 None:intergenic
CTATGACCTCCATAAAGGTG+AGG 0.632142 8.2:-47814434 None:intergenic
GTACCAGTCCTAGAATGAGA+AGG 0.633102 8.2:+47814597 MS.gene032302:CDS
GAAGATACAACAATCTCTAG+TGG 0.636864 8.2:+47814017 MS.gene032302:CDS
TTATAAGGAGGTGGTCGATG+AGG 0.637282 8.2:-47814299 None:intergenic
AGATCACGGCTTTACAAACC+GGG 0.639253 8.2:-47814190 None:intergenic
GGAATCAGCAAATGATCCTG+TGG 0.647430 8.2:+47814073 MS.gene032302:CDS
TGACCACAGAGTGTAACCAC+AGG 0.655445 8.2:-47814089 None:intergenic
TTGGGGAGATGGTGTCGACA+AGG 0.666430 8.2:+47814525 MS.gene032302:CDS
TACATCCCTCACCTTTATGG+AGG 0.669481 8.2:+47814428 MS.gene032302:CDS
GATCCTGTGGTTACACTCTG+TGG 0.698699 8.2:+47814086 MS.gene032302:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATAGCATTTAAGTTATA+AGG - chr8.2:47814317-47814336 None:intergenic 20.0%
!! ATAGCATTTAAGTTATAAGG+AGG - chr8.2:47814314-47814333 None:intergenic 25.0%
AACCTGTAGTTTATTGGATT+CGG - chr8.2:47814267-47814286 None:intergenic 30.0%
AAGTCTCACTAAAGCAAAAA+TGG + chr8.2:47813982-47814001 MS.gene032302:CDS 30.0%
CTTTGATTGCAATATTTGCT+TGG + chr8.2:47814052-47814071 MS.gene032302:CDS 30.0%
TAAACCAACCTGTAGTTTAT+TGG - chr8.2:47814273-47814292 None:intergenic 30.0%
TTAAGCCACTTATATATGCA+TGG - chr8.2:47814128-47814147 None:intergenic 30.0%
TTTCTGAATTAGATTTCCCT+CGG - chr8.2:47814244-47814263 None:intergenic 30.0%
!! ATGTAGTGCTGATAATGAAT+TGG - chr8.2:47814413-47814432 None:intergenic 30.0%
AAAGTGGGACTAATGAAGTA+TGG - chr8.2:47814217-47814236 None:intergenic 35.0%
AACCGAATCCAATAAACTAC+AGG + chr8.2:47814262-47814281 MS.gene032302:CDS 35.0%
AAGTGGGACTAATGAAGTAT+GGG - chr8.2:47814216-47814235 None:intergenic 35.0%
ACCAAGATAATGTAACCCAT+TGG - chr8.2:47814462-47814481 None:intergenic 35.0%
ACTTCATTAGTCCCACTTTA+CGG + chr8.2:47814218-47814237 MS.gene032302:CDS 35.0%
GAAGATACAACAATCTCTAG+TGG + chr8.2:47814017-47814036 MS.gene032302:CDS 35.0%
GAATCCAATAAACTACAGGT+TGG + chr8.2:47814266-47814285 MS.gene032302:CDS 35.0%
GATTGTGACTGGAAATAGTT+CGG - chr8.2:47814386-47814405 None:intergenic 35.0%
GATTGTTGTATCTTCTGTAG+TGG - chr8.2:47814012-47814031 None:intergenic 35.0%
TAATGAAGTATGGGAGATCA+CGG - chr8.2:47814207-47814226 None:intergenic 35.0%
! GCATTTAAGTTATAAGGAGG+TGG - chr8.2:47814311-47814330 None:intergenic 35.0%
!! AATGGGTTACATTATCTTGG+TGG + chr8.2:47814461-47814480 MS.gene032302:CDS 35.0%
AGTTTCGTCAATCCTGTGAT+GGG + chr8.2:47814497-47814516 MS.gene032302:CDS 40.0%
ATTTCCTGCCTTCTCATTCT+AGG - chr8.2:47814608-47814627 None:intergenic 40.0%
CCTCGTGATGTAGATTTGAT+GGG - chr8.2:47814352-47814371 None:intergenic 40.0%
TAGTTTCGTCAATCCTGTGA+TGG + chr8.2:47814496-47814515 MS.gene032302:CDS 40.0%
TATGACCTCCATAAAGGTGA+GGG - chr8.2:47814436-47814455 None:intergenic 40.0%
TCCTCGTGATGTAGATTTGA+TGG - chr8.2:47814353-47814372 None:intergenic 40.0%
TTGTTTGCGTACCCTCATAA+CGG + chr8.2:47814572-47814591 MS.gene032302:CDS 40.0%
!! AATCCTGTGATGGGTTTGTT+TGG + chr8.2:47814506-47814525 MS.gene032302:CDS 40.0%
!! ATCCTGTGATGGGTTTGTTT+GGG + chr8.2:47814507-47814526 MS.gene032302:CDS 40.0%
!! GCCAATGGGTTACATTATCT+TGG + chr8.2:47814458-47814477 MS.gene032302:CDS 40.0%
AGATCACGGCTTTACAAACC+GGG - chr8.2:47814193-47814212 None:intergenic 45.0%
ATATGCATGGCCAGCAATAC+AGG - chr8.2:47814115-47814134 None:intergenic 45.0%
ATGGAGGTCATAGTGCCAAT+GGG + chr8.2:47814444-47814463 MS.gene032302:CDS 45.0%
CACTACATCCCTCACCTTTA+TGG + chr8.2:47814425-47814444 MS.gene032302:CDS 45.0%
CCAAAGCTCTTCTGTTGAAC+TGG + chr8.2:47814151-47814170 MS.gene032302:CDS 45.0%
CCAGTTCAACAGAAGAGCTT+TGG - chr8.2:47814154-47814173 None:intergenic 45.0%
CCCATCAAATCTACATCACG+AGG + chr8.2:47814349-47814368 MS.gene032302:CDS 45.0%
CTATGACCTCCATAAAGGTG+AGG - chr8.2:47814437-47814456 None:intergenic 45.0%
GACTGGTACTTCCGTTATGA+GGG - chr8.2:47814586-47814605 None:intergenic 45.0%
GAGATCACGGCTTTACAAAC+CGG - chr8.2:47814194-47814213 None:intergenic 45.0%
GCTGGCCATGCATATATAAG+TGG + chr8.2:47814120-47814139 MS.gene032302:CDS 45.0%
GGAATCAGCAAATGATCCTG+TGG + chr8.2:47814073-47814092 MS.gene032302:CDS 45.0%
GTACCAGTCCTAGAATGAGA+AGG + chr8.2:47814597-47814616 MS.gene032302:CDS 45.0%
TACATCCCTCACCTTTATGG+AGG + chr8.2:47814428-47814447 MS.gene032302:CDS 45.0%
TATGGAGGTCATAGTGCCAA+TGG + chr8.2:47814443-47814462 MS.gene032302:CDS 45.0%
TGGCACTATGACCTCCATAA+AGG - chr8.2:47814442-47814461 None:intergenic 45.0%
TTATAAGGAGGTGGTCGATG+AGG - chr8.2:47814302-47814321 None:intergenic 45.0%
!! ATGGTGTCGACAAGGATGTT+TGG + chr8.2:47814533-47814552 MS.gene032302:CDS 45.0%
!! TCCTGTGATGGGTTTGTTTG+GGG + chr8.2:47814508-47814527 MS.gene032302:CDS 45.0%
ATTTCCCTCGGCCGTAAAGT+GGG - chr8.2:47814232-47814251 None:intergenic 50.0%
CAGTCCTAGAATGAGAAGGC+AGG + chr8.2:47814601-47814620 MS.gene032302:CDS 50.0%
GAATTGGTCGCGATTGTGAC+TGG - chr8.2:47814397-47814416 None:intergenic 50.0%
GATCCTGTGGTTACACTCTG+TGG + chr8.2:47814086-47814105 MS.gene032302:CDS 50.0%
GCTTTACAAACCGGGCATGT+TGG - chr8.2:47814185-47814204 None:intergenic 50.0%
TCCCCAAACAAACCCATCAC+AGG - chr8.2:47814512-47814531 None:intergenic 50.0%
TCTGTGGTCACCTGTATTGC+TGG + chr8.2:47814102-47814121 MS.gene032302:CDS 50.0%
TGACCACAGAGTGTAACCAC+AGG - chr8.2:47814092-47814111 None:intergenic 50.0%
! GATGGGTTTGTTTGGGGAGA+TGG + chr8.2:47814514-47814533 MS.gene032302:CDS 50.0%
! GGACTGGTACTTCCGTTATG+AGG - chr8.2:47814587-47814606 None:intergenic 50.0%
!! CTGCCTTCTCATTCTAGGAC+TGG - chr8.2:47814603-47814622 None:intergenic 50.0%
GATTTCCCTCGGCCGTAAAG+TGG - chr8.2:47814233-47814252 None:intergenic 55.0%
TAGTCCCACTTTACGGCCGA+GGG + chr8.2:47814225-47814244 MS.gene032302:CDS 55.0%
TTAGTCCCACTTTACGGCCG+AGG + chr8.2:47814224-47814243 MS.gene032302:CDS 55.0%
!! TTGGGGAGATGGTGTCGACA+AGG + chr8.2:47814525-47814544 MS.gene032302:CDS 55.0%
GGATACGCAGCCAACATGCC+CGG + chr8.2:47814172-47814191 MS.gene032302:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 47813951 47814703 47813951 ID=MS.gene032302
chr8.2 mRNA 47813951 47814703 47813951 ID=MS.gene032302.t1;Parent=MS.gene032302
chr8.2 exon 47813951 47814703 47813951 ID=MS.gene032302.t1.exon1;Parent=MS.gene032302.t1
chr8.2 CDS 47813951 47814703 47813951 ID=cds.MS.gene032302.t1;Parent=MS.gene032302.t1
Gene Sequence

>MS.gene032302

ATGGAATCTACATGCTTTGGTTCTAATGAAGAAGTCTCACTAAAGCAAAAATGGAAATCCACTACAGAAGATACAACAATCTCTAGTGGTGAAAATAACTGCTTTGATTGCAATATTTGCTTGGAATCAGCAAATGATCCTGTGGTTACACTCTGTGGTCACCTGTATTGCTGGCCATGCATATATAAGTGGCTTAATGTCCAAAGCTCTTCTGTTGAACTGGATACGCAGCCAACATGCCCGGTTTGTAAAGCCGTGATCTCCCATACTTCATTAGTCCCACTTTACGGCCGAGGGAAATCTAATTCAGAAACCGAATCCAATAAACTACAGGTTGGTTTAAGTATACCTCATCGACCACCTCCTTATAACTTAAATGCTATGTTAACTTCAAATCGCCCATCAAATCTACATCACGAGGAGCAACTTCATCCGAACTATTTCCAGTCACAATCGCGACCAATTCATTATCAGCACTACATCCCTCACCTTTATGGAGGTCATAGTGCCAATGGGTTACATTATCTTGGTGGTGCTGCTATGACTAGTTTCGTCAATCCTGTGATGGGTTTGTTTGGGGAGATGGTGTCGACAAGGATGTTTGGTGTATCTGATGCAAATTTGTTTGCGTACCCTCATAACGGAAGTACCAGTCCTAGAATGAGAAGGCAGGAAATGCAGATTGACAAGTCTTTGAACAGAGTTTCAATCTTTCTTCTTTGTTGTTTCATTTTGTGTCTTCTCTTGTTTTGA

Protein sequence

>MS.gene032302.t1

MESTCFGSNEEVSLKQKWKSTTEDTTISSGENNCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQSSSVELDTQPTCPVCKAVISHTSLVPLYGRGKSNSETESNKLQVGLSIPHRPPPYNLNAMLTSNRPSNLHHEEQLHPNYFQSQSRPIHYQHYIPHLYGGHSANGLHYLGGAAMTSFVNPVMGLFGEMVSTRMFGVSDANLFAYPHNGSTSPRMRRQEMQIDKSLNRVSIFLLCCFILCLLLF