AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36072

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36072.t1 MTR_8g465910 44.269 253 102 11 10 250 19 244 3.62e-61 192
MS.gene36072.t1 MTR_2g090295 40.496 242 107 8 10 238 18 235 2.40e-51 167
MS.gene36072.t1 MTR_4g088875 39.224 232 115 7 11 236 6 217 1.22e-45 152
MS.gene36072.t1 MTR_4g088870 36.179 246 99 6 11 250 6 199 1.21e-43 146
MS.gene36072.t1 MTR_2g013550 42.466 219 114 6 21 236 13 222 1.57e-42 145
MS.gene36072.t1 MTR_2g090330 60.674 89 33 1 32 120 30 116 3.10e-33 119
MS.gene36072.t1 MTR_2g090330 60.674 89 33 1 32 120 27 113 3.57e-33 118
MS.gene36072.t1 MTR_2g090325 48.361 122 40 4 35 146 18 126 1.05e-29 110
MS.gene36072.t1 MTR_1g021895 56.977 86 30 2 35 120 30 108 1.13e-29 111
MS.gene36072.t1 MTR_5g019300 51.648 91 37 2 30 120 30 113 2.43e-27 105
MS.gene36072.t1 MTR_1g054280 41.593 113 60 2 8 120 49 155 9.89e-24 96.7
MS.gene36072.t1 MTR_7g093390 43.810 105 48 3 16 120 153 246 2.23e-22 95.5
MS.gene36072.t1 MTR_1g054210 35.556 135 77 4 8 139 93 220 4.33e-21 89.0
MS.gene36072.t1 MTR_4g029440 38.776 98 54 1 23 120 114 205 5.56e-21 91.3
MS.gene36072.t1 MTR_4g029440 38.776 98 54 1 23 120 120 211 5.99e-21 91.3
MS.gene36072.t1 MTR_1g054260 37.500 128 66 4 19 137 65 187 6.82e-21 87.8
MS.gene36072.t1 MTR_1g054465 35.398 113 70 2 11 120 31 143 9.34e-20 84.3
MS.gene36072.t1 MTR_2g061230 40.909 88 46 1 33 120 105 186 2.37e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36072.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.13e-59 188
MS.gene36072.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.13e-59 188
MS.gene36072.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.13e-59 188
MS.gene36072.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.13e-59 188
MS.gene36072.t1 AT4G27470 49.123 228 90 10 15 236 22 229 1.01e-52 171
MS.gene36072.t1 AT4G28270 35.685 241 94 8 19 250 5 193 8.19e-40 136
MS.gene36072.t1 AT2G23780 33.333 264 112 11 9 248 3 226 2.37e-30 113
MS.gene36072.t1 AT2G23780 33.333 264 112 11 9 248 3 226 2.37e-30 113
MS.gene36072.t1 AT2G23780 33.333 264 112 11 9 248 3 226 2.37e-30 113
MS.gene36072.t1 AT1G19310 56.977 86 30 2 35 120 21 99 4.36e-29 109
MS.gene36072.t1 AT1G74990 53.061 98 36 3 26 120 5 95 1.61e-28 105
MS.gene36072.t1 AT3G58030 42.857 98 45 3 24 120 125 212 4.12e-22 94.7
MS.gene36072.t1 AT3G58030 42.857 98 45 3 24 120 125 212 4.12e-22 94.7
MS.gene36072.t1 AT3G58030 42.857 98 45 3 24 120 125 212 4.12e-22 94.7
MS.gene36072.t1 AT3G58030 42.857 98 45 3 24 120 125 212 4.12e-22 94.7
MS.gene36072.t1 AT2G42030 44.444 99 41 3 35 131 139 225 1.35e-20 90.5
MS.gene36072.t1 AT2G44410 40.000 90 40 2 35 120 123 202 5.47e-18 82.8

Find 67 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACCAACCTGTAGTTTAT+TGG 0.128251 8.1:-53024505 None:intergenic
ATTTCCTGCCTTCTCATTCT+AGG 0.172452 8.1:-53024840 None:intergenic
CACTACATCCCTCACCTTTA+TGG 0.179900 8.1:+53024660 MS.gene36072:CDS
AACATAGCATTTAAGTTATA+AGG 0.203842 8.1:-53024549 None:intergenic
ATCCTGTGATGGGTTTGTTT+GGG 0.307382 8.1:+53024742 MS.gene36072:CDS
ACTTCATTAGTCCCACTTTA+CGG 0.312481 8.1:+53024453 MS.gene36072:CDS
GATGGGTTTGTTTGGGGAGA+TGG 0.320135 8.1:+53024749 MS.gene36072:CDS
AAAGTGGGACTAATGAAGTA+TGG 0.333058 8.1:-53024449 None:intergenic
AATCCTGTGATGGGTTTGTT+TGG 0.347652 8.1:+53024741 MS.gene36072:CDS
TCAATGGAATCTACATGCTT+TGG 0.371719 8.1:+53024183 None:intergenic
GGGCATGTCGGCTGCGTATC+CGG 0.380505 8.1:-53024405 None:intergenic
AACCTGTAGTTTATTGGATT+CGG 0.384366 8.1:-53024499 None:intergenic
GCCAATGGGTTACATTATCT+TGG 0.409333 8.1:+53024693 MS.gene36072:CDS
CCGGTTCAACAGAAGAGCTT+TGG 0.411833 8.1:-53024386 None:intergenic
ATATGCATGGCCAGCAATAC+AGG 0.414858 8.1:-53024347 None:intergenic
TCTGTGGTCACCTGTATTGC+TGG 0.419618 8.1:+53024337 MS.gene36072:CDS
CCAAAGCTCTTCTGTTGAAC+CGG 0.431128 8.1:+53024386 MS.gene36072:CDS
CTTTGATTGCAATATTTGCT+TGG 0.432126 8.1:+53024287 MS.gene36072:CDS
TCGTCGTGATGTAGATTTGA+TGG 0.432516 8.1:-53024585 None:intergenic
CTGCCTTCTCATTCTAGGAC+TGG 0.437502 8.1:-53024835 None:intergenic
GGACTGGTACTTCCGTTATG+AGG 0.441104 8.1:-53024819 None:intergenic
TGGCACTATGACCTCCATAA+AGG 0.443573 8.1:-53024674 None:intergenic
GAGATCATGGCTTTACAAAC+CGG 0.451599 8.1:-53024426 None:intergenic
GAATTGGTCGCGATTGTGAC+TGG 0.479790 8.1:-53024629 None:intergenic
TAGTTTCGTCAATCCTGTGA+TGG 0.480852 8.1:+53024731 MS.gene36072:CDS
TAATGAAGTATGGGAGATCA+TGG 0.500952 8.1:-53024439 None:intergenic
ATGGTGTCGACAAGGATGTT+TGG 0.510014 8.1:+53024768 MS.gene36072:CDS
GATTTCCCTCGGCCGTAAAG+TGG 0.513776 8.1:-53024465 None:intergenic
TATGGAGGTCATAGTGCCAA+TGG 0.516603 8.1:+53024678 MS.gene36072:CDS
AACCGAATCCAATAAACTAC+AGG 0.526244 8.1:+53024497 MS.gene36072:CDS
AGTTTCGTCAATCCTGTGAT+GGG 0.527399 8.1:+53024732 MS.gene36072:CDS
GATTGTGACTGGAAATAGTT+CGG 0.527943 8.1:-53024618 None:intergenic
GATTGTTGTATCTTCTGTAG+TGG 0.527970 8.1:-53024244 None:intergenic
ATGGAGGTCATAGTGCCAAT+GGG 0.535195 8.1:+53024679 MS.gene36072:CDS
GCATTTAAGTTATAAGGAGG+TGG 0.545819 8.1:-53024543 None:intergenic
TTTCTGAATTAGATTTCCCT+CGG 0.545966 8.1:-53024476 None:intergenic
TTAGTCCCACTTTACGGCCG+AGG 0.552806 8.1:+53024459 MS.gene36072:CDS
GCTGGCCATGCATATATAAG+TGG 0.555073 8.1:+53024355 MS.gene36072:CDS
GGATACGCAGCCGACATGCC+CGG 0.556594 8.1:+53024407 MS.gene36072:CDS
AAGTGGGACTAATGAAGTAT+GGG 0.558591 8.1:-53024448 None:intergenic
TCCTGTGATGGGTTTGTTTG+GGG 0.558787 8.1:+53024743 MS.gene36072:CDS
TTGTTTGCGTACCCTCATAA+CGG 0.562898 8.1:+53024807 MS.gene36072:CDS
TCCCCAAACAAACCCATCAC+AGG 0.565091 8.1:-53024744 None:intergenic
ATGTAGTGCTGATAATGAAT+TGG 0.567201 8.1:-53024645 None:intergenic
AATGGGTTACATTATCTTGG+TGG 0.568227 8.1:+53024696 MS.gene36072:CDS
TAGTCCCACTTTACGGCCGA+GGG 0.568488 8.1:+53024460 MS.gene36072:CDS
ATTTCCCTCGGCCGTAAAGT+GGG 0.571345 8.1:-53024464 None:intergenic
GACTGGTACTTCCGTTATGA+GGG 0.589526 8.1:-53024818 None:intergenic
GAATCCAATAAACTACAGGT+TGG 0.591477 8.1:+53024501 MS.gene36072:CDS
TTAAGCCACTTATATATGCA+TGG 0.597413 8.1:-53024360 None:intergenic
CAGTCCTAGAATGAGAAGGC+AGG 0.602401 8.1:+53024836 MS.gene36072:CDS
GCTTTACAAACCGGGCATGT+CGG 0.604579 8.1:-53024417 None:intergenic
ACCAAGATAATGTAACCCAT+TGG 0.617703 8.1:-53024694 None:intergenic
AGATCATGGCTTTACAAACC+GGG 0.622380 8.1:-53024425 None:intergenic
CGTCGTGATGTAGATTTGAT+GGG 0.623072 8.1:-53024584 None:intergenic
ATAGCATTTAAGTTATAAGG+AGG 0.631590 8.1:-53024546 None:intergenic
TATGACCTCCATAAAGGTGA+GGG 0.631991 8.1:-53024668 None:intergenic
CTATGACCTCCATAAAGGTG+AGG 0.632142 8.1:-53024669 None:intergenic
GTACCAGTCCTAGAATGAGA+AGG 0.633102 8.1:+53024832 MS.gene36072:CDS
GAAGATACAACAATCTCTAG+TGG 0.636864 8.1:+53024252 MS.gene36072:CDS
TTATAAGGAGGTGGTCGATG+AGG 0.637282 8.1:-53024534 None:intergenic
GGAATCAGCAAATGATCCTG+TGG 0.647430 8.1:+53024308 MS.gene36072:CDS
TGACCACAGAGTGTAACCAC+AGG 0.655445 8.1:-53024324 None:intergenic
TTGGGGAGATGGTGTCGACA+AGG 0.666430 8.1:+53024760 MS.gene36072:CDS
TACATCCCTCACCTTTATGG+AGG 0.669481 8.1:+53024663 MS.gene36072:CDS
TACATGCTTTGGTTCTAACG+AGG 0.683462 8.1:+53024194 MS.gene36072:CDS
GATCCTGTGGTTACACTCTG+TGG 0.698699 8.1:+53024321 MS.gene36072:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATAGCATTTAAGTTATA+AGG - chr8.1:53024552-53024571 None:intergenic 20.0%
!! ATAGCATTTAAGTTATAAGG+AGG - chr8.1:53024549-53024568 None:intergenic 25.0%
AACCTGTAGTTTATTGGATT+CGG - chr8.1:53024502-53024521 None:intergenic 30.0%
AAGTCTCACTAAAGCAAAAA+TGG + chr8.1:53024217-53024236 MS.gene36072:CDS 30.0%
CTTTGATTGCAATATTTGCT+TGG + chr8.1:53024287-53024306 MS.gene36072:CDS 30.0%
TAAACCAACCTGTAGTTTAT+TGG - chr8.1:53024508-53024527 None:intergenic 30.0%
TTAAGCCACTTATATATGCA+TGG - chr8.1:53024363-53024382 None:intergenic 30.0%
TTTCTGAATTAGATTTCCCT+CGG - chr8.1:53024479-53024498 None:intergenic 30.0%
!! ATGTAGTGCTGATAATGAAT+TGG - chr8.1:53024648-53024667 None:intergenic 30.0%
AAAGTGGGACTAATGAAGTA+TGG - chr8.1:53024452-53024471 None:intergenic 35.0%
AACCGAATCCAATAAACTAC+AGG + chr8.1:53024497-53024516 MS.gene36072:CDS 35.0%
AAGTGGGACTAATGAAGTAT+GGG - chr8.1:53024451-53024470 None:intergenic 35.0%
ACCAAGATAATGTAACCCAT+TGG - chr8.1:53024697-53024716 None:intergenic 35.0%
ACTTCATTAGTCCCACTTTA+CGG + chr8.1:53024453-53024472 MS.gene36072:CDS 35.0%
GAAGATACAACAATCTCTAG+TGG + chr8.1:53024252-53024271 MS.gene36072:CDS 35.0%
GAATCCAATAAACTACAGGT+TGG + chr8.1:53024501-53024520 MS.gene36072:CDS 35.0%
GATTGTGACTGGAAATAGTT+CGG - chr8.1:53024621-53024640 None:intergenic 35.0%
GATTGTTGTATCTTCTGTAG+TGG - chr8.1:53024247-53024266 None:intergenic 35.0%
TAATGAAGTATGGGAGATCA+TGG - chr8.1:53024442-53024461 None:intergenic 35.0%
! GCATTTAAGTTATAAGGAGG+TGG - chr8.1:53024546-53024565 None:intergenic 35.0%
!! AATGGGTTACATTATCTTGG+TGG + chr8.1:53024696-53024715 MS.gene36072:CDS 35.0%
AGTTTCGTCAATCCTGTGAT+GGG + chr8.1:53024732-53024751 MS.gene36072:CDS 40.0%
ATTTCCTGCCTTCTCATTCT+AGG - chr8.1:53024843-53024862 None:intergenic 40.0%
CGTCGTGATGTAGATTTGAT+GGG - chr8.1:53024587-53024606 None:intergenic 40.0%
TAGTTTCGTCAATCCTGTGA+TGG + chr8.1:53024731-53024750 MS.gene36072:CDS 40.0%
TATGACCTCCATAAAGGTGA+GGG - chr8.1:53024671-53024690 None:intergenic 40.0%
TCGTCGTGATGTAGATTTGA+TGG - chr8.1:53024588-53024607 None:intergenic 40.0%
TTGTTTGCGTACCCTCATAA+CGG + chr8.1:53024807-53024826 MS.gene36072:CDS 40.0%
! AGATCATGGCTTTACAAACC+GGG - chr8.1:53024428-53024447 None:intergenic 40.0%
! GAGATCATGGCTTTACAAAC+CGG - chr8.1:53024429-53024448 None:intergenic 40.0%
!! AATCCTGTGATGGGTTTGTT+TGG + chr8.1:53024741-53024760 MS.gene36072:CDS 40.0%
!! ATCCTGTGATGGGTTTGTTT+GGG + chr8.1:53024742-53024761 MS.gene36072:CDS 40.0%
!! GCCAATGGGTTACATTATCT+TGG + chr8.1:53024693-53024712 MS.gene36072:CDS 40.0%
!! TACATGCTTTGGTTCTAACG+AGG + chr8.1:53024194-53024213 MS.gene36072:CDS 40.0%
ATATGCATGGCCAGCAATAC+AGG - chr8.1:53024350-53024369 None:intergenic 45.0%
ATGGAGGTCATAGTGCCAAT+GGG + chr8.1:53024679-53024698 MS.gene36072:CDS 45.0%
CACTACATCCCTCACCTTTA+TGG + chr8.1:53024660-53024679 MS.gene36072:CDS 45.0%
CCAAAGCTCTTCTGTTGAAC+CGG + chr8.1:53024386-53024405 MS.gene36072:CDS 45.0%
CTATGACCTCCATAAAGGTG+AGG - chr8.1:53024672-53024691 None:intergenic 45.0%
GACTGGTACTTCCGTTATGA+GGG - chr8.1:53024821-53024840 None:intergenic 45.0%
GCTGGCCATGCATATATAAG+TGG + chr8.1:53024355-53024374 MS.gene36072:CDS 45.0%
GGAATCAGCAAATGATCCTG+TGG + chr8.1:53024308-53024327 MS.gene36072:CDS 45.0%
GTACCAGTCCTAGAATGAGA+AGG + chr8.1:53024832-53024851 MS.gene36072:CDS 45.0%
TACATCCCTCACCTTTATGG+AGG + chr8.1:53024663-53024682 MS.gene36072:CDS 45.0%
TATGGAGGTCATAGTGCCAA+TGG + chr8.1:53024678-53024697 MS.gene36072:CDS 45.0%
TGGCACTATGACCTCCATAA+AGG - chr8.1:53024677-53024696 None:intergenic 45.0%
TTATAAGGAGGTGGTCGATG+AGG - chr8.1:53024537-53024556 None:intergenic 45.0%
!! ATGGTGTCGACAAGGATGTT+TGG + chr8.1:53024768-53024787 MS.gene36072:CDS 45.0%
!! TCCTGTGATGGGTTTGTTTG+GGG + chr8.1:53024743-53024762 MS.gene36072:CDS 45.0%
ATTTCCCTCGGCCGTAAAGT+GGG - chr8.1:53024467-53024486 None:intergenic 50.0%
CAGTCCTAGAATGAGAAGGC+AGG + chr8.1:53024836-53024855 MS.gene36072:CDS 50.0%
CCGGTTCAACAGAAGAGCTT+TGG - chr8.1:53024389-53024408 None:intergenic 50.0%
GAATTGGTCGCGATTGTGAC+TGG - chr8.1:53024632-53024651 None:intergenic 50.0%
GATCCTGTGGTTACACTCTG+TGG + chr8.1:53024321-53024340 MS.gene36072:CDS 50.0%
GCTTTACAAACCGGGCATGT+CGG - chr8.1:53024420-53024439 None:intergenic 50.0%
TCCCCAAACAAACCCATCAC+AGG - chr8.1:53024747-53024766 None:intergenic 50.0%
TCTGTGGTCACCTGTATTGC+TGG + chr8.1:53024337-53024356 MS.gene36072:CDS 50.0%
TGACCACAGAGTGTAACCAC+AGG - chr8.1:53024327-53024346 None:intergenic 50.0%
! GATGGGTTTGTTTGGGGAGA+TGG + chr8.1:53024749-53024768 MS.gene36072:CDS 50.0%
! GGACTGGTACTTCCGTTATG+AGG - chr8.1:53024822-53024841 None:intergenic 50.0%
!! CTGCCTTCTCATTCTAGGAC+TGG - chr8.1:53024838-53024857 None:intergenic 50.0%
GATTTCCCTCGGCCGTAAAG+TGG - chr8.1:53024468-53024487 None:intergenic 55.0%
TAGTCCCACTTTACGGCCGA+GGG + chr8.1:53024460-53024479 MS.gene36072:CDS 55.0%
TTAGTCCCACTTTACGGCCG+AGG + chr8.1:53024459-53024478 MS.gene36072:CDS 55.0%
!! TTGGGGAGATGGTGTCGACA+AGG + chr8.1:53024760-53024779 MS.gene36072:CDS 55.0%
GGATACGCAGCCGACATGCC+CGG + chr8.1:53024407-53024426 MS.gene36072:CDS 65.0%
! GGGCATGTCGGCTGCGTATC+CGG - chr8.1:53024408-53024427 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 53024186 53024938 53024186 ID=MS.gene36072
chr8.1 mRNA 53024186 53024938 53024186 ID=MS.gene36072.t1;Parent=MS.gene36072
chr8.1 exon 53024186 53024938 53024186 ID=MS.gene36072.t1.exon1;Parent=MS.gene36072.t1
chr8.1 CDS 53024186 53024938 53024186 ID=cds.MS.gene36072.t1;Parent=MS.gene36072.t1
Gene Sequence

>MS.gene36072

ATGGAATCTACATGCTTTGGTTCTAACGAGGAAGTCTCACTAAAGCAAAAATGGAAATCCACTACAGAAGATACAACAATCTCTAGTGGTGAAAATAACTGCTTTGATTGCAATATTTGCTTGGAATCAGCAAATGATCCTGTGGTTACACTCTGTGGTCACCTGTATTGCTGGCCATGCATATATAAGTGGCTTAATGTCCAAAGCTCTTCTGTTGAACCGGATACGCAGCCGACATGCCCGGTTTGTAAAGCCATGATCTCCCATACTTCATTAGTCCCACTTTACGGCCGAGGGAAATCTAATTCAGAAACCGAATCCAATAAACTACAGGTTGGTTTAAGTATACCTCATCGACCACCTCCTTATAACTTAAATGCTATGTTAACTTCAAATCGCCCATCAAATCTACATCACGACGAGCAACTTCATCCGAACTATTTCCAGTCACAATCGCGACCAATTCATTATCAGCACTACATCCCTCACCTTTATGGAGGTCATAGTGCCAATGGGTTACATTATCTTGGTGGTGCTGCTATGACTAGTTTCGTCAATCCTGTGATGGGTTTGTTTGGGGAGATGGTGTCGACAAGGATGTTTGGTGTATCTGATGCAAATTTGTTTGCGTACCCTCATAACGGAAGTACCAGTCCTAGAATGAGAAGGCAGGAAATGCAGATTGACAAGTCTTTGAACAGAGTTTCAATCTTTCTTCTTTGTTGTTTCATTTTGTGTCTTCTCTTGTTTTGA
Protein sequence

>MS.gene36072.t1

MESTCFGSNEEVSLKQKWKSTTEDTTISSGENNCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQSSSVEPDTQPTCPVCKAMISHTSLVPLYGRGKSNSETESNKLQVGLSIPHRPPPYNLNAMLTSNRPSNLHHDEQLHPNYFQSQSRPIHYQHYIPHLYGGHSANGLHYLGGAAMTSFVNPVMGLFGEMVSTRMFGVSDANLFAYPHNGSTSPRMRRQEMQIDKSLNRVSIFLLCCFILCLLLF