AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34211


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34211.t1 MTR_8g465910 44.269 253 102 11 10 250 19 244 1.29e-61 194
MS.gene34211.t1 MTR_2g090295 40.496 242 107 8 10 238 18 235 2.88e-51 167
MS.gene34211.t1 MTR_4g088875 38.793 232 116 7 11 236 6 217 8.69e-45 150
MS.gene34211.t1 MTR_4g088870 35.772 246 100 6 11 250 6 199 1.45e-42 144
MS.gene34211.t1 MTR_2g013550 42.466 219 114 7 21 236 13 222 2.26e-42 144
MS.gene34211.t1 MTR_2g090330 60.674 89 33 1 32 120 30 116 3.17e-33 119
MS.gene34211.t1 MTR_2g090330 60.674 89 33 1 32 120 27 113 3.97e-33 118
MS.gene34211.t1 MTR_2g090325 48.760 121 39 4 35 145 18 125 7.84e-30 110
MS.gene34211.t1 MTR_1g021895 55.814 86 31 2 35 120 30 108 4.90e-29 109
MS.gene34211.t1 MTR_5g019300 51.648 91 37 2 30 120 30 113 2.64e-27 105
MS.gene34211.t1 MTR_1g054280 41.593 113 60 2 8 120 49 155 1.06e-23 96.7
MS.gene34211.t1 MTR_7g093390 43.810 105 48 3 16 120 153 246 2.38e-22 95.5
MS.gene34211.t1 MTR_1g054210 35.556 135 77 4 8 139 93 220 5.33e-21 88.6
MS.gene34211.t1 MTR_4g029440 38.776 98 54 1 23 120 120 211 6.48e-21 91.3
MS.gene34211.t1 MTR_4g029440 38.776 98 54 1 23 120 114 205 6.57e-21 91.3
MS.gene34211.t1 MTR_1g054260 37.500 128 66 4 19 137 65 187 1.07e-20 87.0
MS.gene34211.t1 MTR_1g054465 35.398 113 70 2 11 120 31 143 1.65e-19 84.0
MS.gene34211.t1 MTR_2g061230 40.909 88 46 1 33 120 105 186 2.46e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34211.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene34211.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene34211.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene34211.t1 AT4G03510 43.860 228 100 6 31 250 42 249 3.27e-59 188
MS.gene34211.t1 AT4G27470 49.123 228 90 10 15 236 22 229 2.47e-53 172
MS.gene34211.t1 AT4G28270 35.685 241 94 8 19 250 5 193 6.89e-40 137
MS.gene34211.t1 AT2G23780 34.733 262 111 11 9 248 3 226 2.67e-31 115
MS.gene34211.t1 AT2G23780 34.733 262 111 11 9 248 3 226 2.67e-31 115
MS.gene34211.t1 AT2G23780 34.733 262 111 11 9 248 3 226 2.67e-31 115
MS.gene34211.t1 AT1G19310 58.140 86 29 2 35 120 21 99 1.07e-29 111
MS.gene34211.t1 AT1G74990 53.061 98 36 3 26 120 5 95 2.13e-28 105
MS.gene34211.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene34211.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene34211.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene34211.t1 AT3G58030 35.766 137 73 5 24 155 125 251 1.44e-22 95.9
MS.gene34211.t1 AT2G42030 44.444 99 41 3 35 131 139 225 1.85e-20 90.1
MS.gene34211.t1 AT2G44410 40.000 90 40 2 35 120 123 202 6.14e-18 82.8

Find 66 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACCAACCTGTAGTTTAT+TGG 0.128251 8.4:-48154250 None:intergenic
ATTTCCTGCCTTCTCATTCT+AGG 0.172452 8.4:-48154585 None:intergenic
CACTACATCCCTCACCTTTA+TGG 0.179900 8.4:+48154405 MS.gene34211:CDS
AACATAGCATTTAAGTTATA+AGG 0.203842 8.4:-48154294 None:intergenic
ATCCTGTGATGGGTTTGTTT+GGG 0.307382 8.4:+48154487 MS.gene34211:CDS
ACTTCATTAGTCCCACTTTA+CGG 0.312481 8.4:+48154198 MS.gene34211:CDS
GATGGGTTTGTTTGGGGAGA+TGG 0.320135 8.4:+48154494 MS.gene34211:CDS
AAAGTGGGACTAATGAAGTA+TGG 0.333058 8.4:-48154194 None:intergenic
AATCCTGTGATGGGTTTGTT+TGG 0.347652 8.4:+48154486 MS.gene34211:CDS
TCAATGGAATCTACATGCTT+TGG 0.371719 8.4:+48153928 None:intergenic
AACCTGTAGTTTATTGGATT+CGG 0.384366 8.4:-48154244 None:intergenic
TCCTCGTGATGTAGATTTGA+TGG 0.387604 8.4:-48154330 None:intergenic
CCAAAGCTCTTCTGTTGAAC+TGG 0.390019 8.4:+48154131 MS.gene34211:CDS
GCCAATGGGTTACATTATCT+TGG 0.409333 8.4:+48154438 MS.gene34211:CDS
CCAGTTCAACAGAAGAGCTT+TGG 0.409537 8.4:-48154131 None:intergenic
ATATGCATGGCCAGCAATAC+AGG 0.414858 8.4:-48154092 None:intergenic
TCTGTGGTCACCTGTATTGC+TGG 0.419618 8.4:+48154082 MS.gene34211:CDS
CTTTGATTGCAATATTTGCT+TGG 0.432126 8.4:+48154032 MS.gene34211:CDS
CTGCCTTCTCATTCTAGGAC+TGG 0.437502 8.4:-48154580 None:intergenic
GGACTGGTACTTCCGTTATG+AGG 0.441104 8.4:-48154564 None:intergenic
TGGCACTATGACCTCCATAA+AGG 0.443573 8.4:-48154419 None:intergenic
GAGATCACGGCTTTACAAAC+CGG 0.457250 8.4:-48154171 None:intergenic
GAATTGGTCGCGATTGTGAC+TGG 0.479790 8.4:-48154374 None:intergenic
TAGTTTCGTCAATCCTGTGA+TGG 0.480852 8.4:+48154476 MS.gene34211:CDS
ATGGTGTCGACAAGGATGTT+TGG 0.510014 8.4:+48154513 MS.gene34211:CDS
GATTTCCCTCGGCCGTAAAG+TGG 0.513776 8.4:-48154210 None:intergenic
TATGGAGGTCATAGTGCCAA+TGG 0.516603 8.4:+48154423 MS.gene34211:CDS
AACCGAATCCAATAAACTAC+AGG 0.526244 8.4:+48154242 MS.gene34211:CDS
AGTTTCGTCAATCCTGTGAT+GGG 0.527399 8.4:+48154477 MS.gene34211:CDS
GATTGTGACTGGAAATAGTT+CGG 0.527943 8.4:-48154363 None:intergenic
GATTGTTGTATCTTCTGTAG+TGG 0.527970 8.4:-48153989 None:intergenic
ATGGAGGTCATAGTGCCAAT+GGG 0.535195 8.4:+48154424 MS.gene34211:CDS
GCATTTAAGTTATAAGGAGG+TGG 0.545819 8.4:-48154288 None:intergenic
TTTCTGAATTAGATTTCCCT+CGG 0.545966 8.4:-48154221 None:intergenic
TTAGTCCCACTTTACGGCCG+AGG 0.552806 8.4:+48154204 MS.gene34211:CDS
GCTGGCCATGCATATATAAG+TGG 0.555073 8.4:+48154100 MS.gene34211:CDS
AAGTGGGACTAATGAAGTAT+GGG 0.558591 8.4:-48154193 None:intergenic
TCCTGTGATGGGTTTGTTTG+GGG 0.558787 8.4:+48154488 MS.gene34211:CDS
TTGTTTGCGTACCCTCATAA+CGG 0.562898 8.4:+48154552 MS.gene34211:CDS
TCCCCAAACAAACCCATCAC+AGG 0.565091 8.4:-48154489 None:intergenic
GCTTTACAAACCGGGCATGT+TGG 0.566623 8.4:-48154162 None:intergenic
ATGTAGTGCTGATAATGAAT+TGG 0.567201 8.4:-48154390 None:intergenic
AATGGGTTACATTATCTTGG+TGG 0.568227 8.4:+48154441 MS.gene34211:CDS
TAGTCCCACTTTACGGCCGA+GGG 0.568488 8.4:+48154205 MS.gene34211:CDS
ATTTCCCTCGGCCGTAAAGT+GGG 0.571345 8.4:-48154209 None:intergenic
GGATACGCAGCCAACATGCC+CGG 0.580139 8.4:+48154152 MS.gene34211:CDS
GACTGGTACTTCCGTTATGA+GGG 0.589526 8.4:-48154563 None:intergenic
GAATCCAATAAACTACAGGT+TGG 0.591477 8.4:+48154246 MS.gene34211:CDS
TAATGAAGTATGGGAGATCA+CGG 0.595278 8.4:-48154184 None:intergenic
CCTCGTGATGTAGATTTGAT+GGG 0.597079 8.4:-48154329 None:intergenic
TTAAGCCACTTATATATGCA+TGG 0.597413 8.4:-48154105 None:intergenic
CAGTCCTAGAATGAGAAGGC+AGG 0.602401 8.4:+48154581 MS.gene34211:CDS
ACCAAGATAATGTAACCCAT+TGG 0.617703 8.4:-48154439 None:intergenic
CCCATCAAATCTACATCACG+AGG 0.619297 8.4:+48154329 MS.gene34211:CDS
ATAGCATTTAAGTTATAAGG+AGG 0.631590 8.4:-48154291 None:intergenic
TATGACCTCCATAAAGGTGA+GGG 0.631991 8.4:-48154413 None:intergenic
CTATGACCTCCATAAAGGTG+AGG 0.632142 8.4:-48154414 None:intergenic
GTACCAGTCCTAGAATGAGA+AGG 0.633102 8.4:+48154577 MS.gene34211:CDS
GAAGATACAACAATCTCTAG+TGG 0.636864 8.4:+48153997 MS.gene34211:CDS
TTATAAGGAGGTGGTCGATG+AGG 0.637282 8.4:-48154279 None:intergenic
AGATCACGGCTTTACAAACC+GGG 0.639253 8.4:-48154170 None:intergenic
GGAATCAGCAAATGATCCTG+TGG 0.647430 8.4:+48154053 MS.gene34211:CDS
TGACCACAGAGTGTAACCAC+AGG 0.655445 8.4:-48154069 None:intergenic
TTGGGGAGATGGTGTCGACA+AGG 0.666430 8.4:+48154505 MS.gene34211:CDS
TACATCCCTCACCTTTATGG+AGG 0.669481 8.4:+48154408 MS.gene34211:CDS
GATCCTGTGGTTACACTCTG+TGG 0.698699 8.4:+48154066 MS.gene34211:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATAGCATTTAAGTTATA+AGG - chr8.4:48154297-48154316 None:intergenic 20.0%
!! ATAGCATTTAAGTTATAAGG+AGG - chr8.4:48154294-48154313 None:intergenic 25.0%
AACCTGTAGTTTATTGGATT+CGG - chr8.4:48154247-48154266 None:intergenic 30.0%
AAGTCTCACTAAAGCAAAAA+TGG + chr8.4:48153962-48153981 MS.gene34211:CDS 30.0%
CTTTGATTGCAATATTTGCT+TGG + chr8.4:48154032-48154051 MS.gene34211:CDS 30.0%
TAAACCAACCTGTAGTTTAT+TGG - chr8.4:48154253-48154272 None:intergenic 30.0%
TTAAGCCACTTATATATGCA+TGG - chr8.4:48154108-48154127 None:intergenic 30.0%
TTTCTGAATTAGATTTCCCT+CGG - chr8.4:48154224-48154243 None:intergenic 30.0%
!! ATGTAGTGCTGATAATGAAT+TGG - chr8.4:48154393-48154412 None:intergenic 30.0%
AAAGTGGGACTAATGAAGTA+TGG - chr8.4:48154197-48154216 None:intergenic 35.0%
AACCGAATCCAATAAACTAC+AGG + chr8.4:48154242-48154261 MS.gene34211:CDS 35.0%
AAGTGGGACTAATGAAGTAT+GGG - chr8.4:48154196-48154215 None:intergenic 35.0%
ACCAAGATAATGTAACCCAT+TGG - chr8.4:48154442-48154461 None:intergenic 35.0%
ACTTCATTAGTCCCACTTTA+CGG + chr8.4:48154198-48154217 MS.gene34211:CDS 35.0%
GAAGATACAACAATCTCTAG+TGG + chr8.4:48153997-48154016 MS.gene34211:CDS 35.0%
GAATCCAATAAACTACAGGT+TGG + chr8.4:48154246-48154265 MS.gene34211:CDS 35.0%
GATTGTGACTGGAAATAGTT+CGG - chr8.4:48154366-48154385 None:intergenic 35.0%
GATTGTTGTATCTTCTGTAG+TGG - chr8.4:48153992-48154011 None:intergenic 35.0%
TAATGAAGTATGGGAGATCA+CGG - chr8.4:48154187-48154206 None:intergenic 35.0%
! GCATTTAAGTTATAAGGAGG+TGG - chr8.4:48154291-48154310 None:intergenic 35.0%
!! AATGGGTTACATTATCTTGG+TGG + chr8.4:48154441-48154460 MS.gene34211:CDS 35.0%
AGTTTCGTCAATCCTGTGAT+GGG + chr8.4:48154477-48154496 MS.gene34211:CDS 40.0%
ATTTCCTGCCTTCTCATTCT+AGG - chr8.4:48154588-48154607 None:intergenic 40.0%
CCTCGTGATGTAGATTTGAT+GGG - chr8.4:48154332-48154351 None:intergenic 40.0%
TAGTTTCGTCAATCCTGTGA+TGG + chr8.4:48154476-48154495 MS.gene34211:CDS 40.0%
TATGACCTCCATAAAGGTGA+GGG - chr8.4:48154416-48154435 None:intergenic 40.0%
TCCTCGTGATGTAGATTTGA+TGG - chr8.4:48154333-48154352 None:intergenic 40.0%
TTGTTTGCGTACCCTCATAA+CGG + chr8.4:48154552-48154571 MS.gene34211:CDS 40.0%
!! AATCCTGTGATGGGTTTGTT+TGG + chr8.4:48154486-48154505 MS.gene34211:CDS 40.0%
!! ATCCTGTGATGGGTTTGTTT+GGG + chr8.4:48154487-48154506 MS.gene34211:CDS 40.0%
!! GCCAATGGGTTACATTATCT+TGG + chr8.4:48154438-48154457 MS.gene34211:CDS 40.0%
AGATCACGGCTTTACAAACC+GGG - chr8.4:48154173-48154192 None:intergenic 45.0%
ATATGCATGGCCAGCAATAC+AGG - chr8.4:48154095-48154114 None:intergenic 45.0%
ATGGAGGTCATAGTGCCAAT+GGG + chr8.4:48154424-48154443 MS.gene34211:CDS 45.0%
CACTACATCCCTCACCTTTA+TGG + chr8.4:48154405-48154424 MS.gene34211:CDS 45.0%
CCAAAGCTCTTCTGTTGAAC+TGG + chr8.4:48154131-48154150 MS.gene34211:CDS 45.0%
CCAGTTCAACAGAAGAGCTT+TGG - chr8.4:48154134-48154153 None:intergenic 45.0%
CCCATCAAATCTACATCACG+AGG + chr8.4:48154329-48154348 MS.gene34211:CDS 45.0%
CTATGACCTCCATAAAGGTG+AGG - chr8.4:48154417-48154436 None:intergenic 45.0%
GACTGGTACTTCCGTTATGA+GGG - chr8.4:48154566-48154585 None:intergenic 45.0%
GAGATCACGGCTTTACAAAC+CGG - chr8.4:48154174-48154193 None:intergenic 45.0%
GCTGGCCATGCATATATAAG+TGG + chr8.4:48154100-48154119 MS.gene34211:CDS 45.0%
GGAATCAGCAAATGATCCTG+TGG + chr8.4:48154053-48154072 MS.gene34211:CDS 45.0%
GTACCAGTCCTAGAATGAGA+AGG + chr8.4:48154577-48154596 MS.gene34211:CDS 45.0%
TACATCCCTCACCTTTATGG+AGG + chr8.4:48154408-48154427 MS.gene34211:CDS 45.0%
TATGGAGGTCATAGTGCCAA+TGG + chr8.4:48154423-48154442 MS.gene34211:CDS 45.0%
TGGCACTATGACCTCCATAA+AGG - chr8.4:48154422-48154441 None:intergenic 45.0%
TTATAAGGAGGTGGTCGATG+AGG - chr8.4:48154282-48154301 None:intergenic 45.0%
!! ATGGTGTCGACAAGGATGTT+TGG + chr8.4:48154513-48154532 MS.gene34211:CDS 45.0%
!! TCCTGTGATGGGTTTGTTTG+GGG + chr8.4:48154488-48154507 MS.gene34211:CDS 45.0%
ATTTCCCTCGGCCGTAAAGT+GGG - chr8.4:48154212-48154231 None:intergenic 50.0%
CAGTCCTAGAATGAGAAGGC+AGG + chr8.4:48154581-48154600 MS.gene34211:CDS 50.0%
GAATTGGTCGCGATTGTGAC+TGG - chr8.4:48154377-48154396 None:intergenic 50.0%
GATCCTGTGGTTACACTCTG+TGG + chr8.4:48154066-48154085 MS.gene34211:CDS 50.0%
GCTTTACAAACCGGGCATGT+TGG - chr8.4:48154165-48154184 None:intergenic 50.0%
TCCCCAAACAAACCCATCAC+AGG - chr8.4:48154492-48154511 None:intergenic 50.0%
TCTGTGGTCACCTGTATTGC+TGG + chr8.4:48154082-48154101 MS.gene34211:CDS 50.0%
TGACCACAGAGTGTAACCAC+AGG - chr8.4:48154072-48154091 None:intergenic 50.0%
! GATGGGTTTGTTTGGGGAGA+TGG + chr8.4:48154494-48154513 MS.gene34211:CDS 50.0%
! GGACTGGTACTTCCGTTATG+AGG - chr8.4:48154567-48154586 None:intergenic 50.0%
!! CTGCCTTCTCATTCTAGGAC+TGG - chr8.4:48154583-48154602 None:intergenic 50.0%
GATTTCCCTCGGCCGTAAAG+TGG - chr8.4:48154213-48154232 None:intergenic 55.0%
TAGTCCCACTTTACGGCCGA+GGG + chr8.4:48154205-48154224 MS.gene34211:CDS 55.0%
TTAGTCCCACTTTACGGCCG+AGG + chr8.4:48154204-48154223 MS.gene34211:CDS 55.0%
!! TTGGGGAGATGGTGTCGACA+AGG + chr8.4:48154505-48154524 MS.gene34211:CDS 55.0%
GGATACGCAGCCAACATGCC+CGG + chr8.4:48154152-48154171 MS.gene34211:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 48153931 48154683 48153931 ID=MS.gene34211
chr8.4 mRNA 48153931 48154683 48153931 ID=MS.gene34211.t1;Parent=MS.gene34211
chr8.4 exon 48153931 48154683 48153931 ID=MS.gene34211.t1.exon1;Parent=MS.gene34211.t1
chr8.4 CDS 48153931 48154683 48153931 ID=cds.MS.gene34211.t1;Parent=MS.gene34211.t1
Gene Sequence

>MS.gene34211

ATGGAATCTACATGCTTTGGTTCTAATGAAGAAGTCTCACTAAAGCAAAAATGGAAATCCACTACAGAAGATACAACAATCTCTAGTGGTGAAAATAACTGCTTTGATTGCAATATTTGCTTGGAATCAGCAAATGATCCTGTGGTTACACTCTGTGGTCACCTGTATTGCTGGCCATGCATATATAAGTGGCTTAATGTCCAAAGCTCTTCTGTTGAACTGGATACGCAGCCAACATGCCCGGTTTGTAAAGCCGTGATCTCCCATACTTCATTAGTCCCACTTTACGGCCGAGGGAAATCTAATTCAGAAACCGAATCCAATAAACTACAGGTTGGTTTAAGTATACCTCATCGACCACCTCCTTATAACTTAAATGCTATGTTAACTTCAAATCGCCCATCAAATCTACATCACGAGGAGCAACTTCATCCGAACTATTTCCAGTCACAATCGCGACCAATTCATTATCAGCACTACATCCCTCACCTTTATGGAGGTCATAGTGCCAATGGGTTACATTATCTTGGTGGTGCTGCTATGACTAGTTTCGTCAATCCTGTGATGGGTTTGTTTGGGGAGATGGTGTCGACAAGGATGTTTGGTGTATCTGATGCAAATTTGTTTGCGTACCCTCATAACGGAAGTACCAGTCCTAGAATGAGAAGGCAGGAAATGCAGATTGACAAGTCTTTGAACAGAGTTTCAATCTTTCTTCTTTGTTGTTTCATTTTGTGTCTTCTCTTGTTTTGA

Protein sequence

>MS.gene34211.t1

MESTCFGSNEEVSLKQKWKSTTEDTTISSGENNCFDCNICLESANDPVVTLCGHLYCWPCIYKWLNVQSSSVELDTQPTCPVCKAVISHTSLVPLYGRGKSNSETESNKLQVGLSIPHRPPPYNLNAMLTSNRPSNLHHEEQLHPNYFQSQSRPIHYQHYIPHLYGGHSANGLHYLGGAAMTSFVNPVMGLFGEMVSTRMFGVSDANLFAYPHNGSTSPRMRRQEMQIDKSLNRVSIFLLCCFILCLLLF