Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053537.t1 | XP_003607927.1 | 99.4 | 181 | 1 | 0 | 2 | 182 | 67 | 247 | 7.40E-99 | 369.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053537.t1 | Q40313 | 99.4 | 181 | 1 | 0 | 2 | 182 | 67 | 247 | 1.8e-101 | 369.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053537.t1 | G7JK14 | 99.4 | 181 | 1 | 0 | 2 | 182 | 67 | 247 | 5.3e-99 | 369.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053535 | MS.gene053537 | 0.964691 | 1.20E-123 | -1.69E-46 |
| MS.gene053537 | MS.gene053539 | 0.923225 | 3.52E-89 | -1.69E-46 |
| MS.gene053537 | MS.gene059423 | 0.967116 | 7.78E-127 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053537.t1 | MTR_4g085590 | 99.448 | 181 | 1 | 0 | 2 | 182 | 67 | 247 | 5.09e-132 | 370 |
| MS.gene053537.t1 | MTR_4g094815 | 71.823 | 181 | 51 | 0 | 2 | 182 | 68 | 248 | 4.38e-98 | 284 |
| MS.gene053537.t1 | MTR_4g094925 | 68.333 | 180 | 57 | 0 | 2 | 181 | 32 | 211 | 3.44e-95 | 275 |
| MS.gene053537.t1 | MTR_4g094925 | 68.333 | 180 | 57 | 0 | 2 | 181 | 63 | 242 | 1.21e-94 | 275 |
| MS.gene053537.t1 | MTR_4g094898 | 70.718 | 181 | 53 | 0 | 2 | 182 | 7 | 187 | 6.40e-91 | 263 |
| MS.gene053537.t1 | MTR_4g094898 | 70.718 | 181 | 53 | 0 | 2 | 182 | 68 | 248 | 3.46e-90 | 264 |
| MS.gene053537.t1 | MTR_4g094812 | 70.000 | 180 | 54 | 0 | 2 | 181 | 66 | 245 | 6.76e-87 | 255 |
| MS.gene053537.t1 | MTR_8g021225 | 59.551 | 178 | 72 | 0 | 3 | 180 | 56 | 233 | 2.45e-74 | 223 |
| MS.gene053537.t1 | MTR_8g101900 | 57.542 | 179 | 75 | 1 | 3 | 181 | 57 | 234 | 2.99e-66 | 202 |
| MS.gene053537.t1 | MTR_6g091680 | 57.542 | 179 | 75 | 1 | 3 | 181 | 60 | 237 | 8.94e-66 | 201 |
| MS.gene053537.t1 | MTR_4g094368 | 52.809 | 178 | 83 | 1 | 2 | 179 | 51 | 227 | 2.28e-63 | 195 |
| MS.gene053537.t1 | MTR_2g070410 | 38.889 | 180 | 101 | 3 | 2 | 180 | 126 | 297 | 9.43e-39 | 134 |
| MS.gene053537.t1 | MTR_2g070410 | 40.299 | 134 | 79 | 1 | 2 | 135 | 126 | 258 | 8.87e-30 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053537.t1 | AT4G34050 | 90.000 | 180 | 18 | 0 | 2 | 181 | 106 | 285 | 1.58e-123 | 350 |
| MS.gene053537.t1 | AT4G34050 | 89.503 | 181 | 19 | 0 | 2 | 182 | 79 | 259 | 2.91e-123 | 348 |
| MS.gene053537.t1 | AT4G34050 | 87.903 | 124 | 15 | 0 | 59 | 182 | 25 | 148 | 2.95e-80 | 235 |
| MS.gene053537.t1 | AT4G26220 | 59.777 | 179 | 71 | 1 | 3 | 181 | 54 | 231 | 3.78e-77 | 230 |
| MS.gene053537.t1 | AT1G67980 | 56.667 | 180 | 74 | 2 | 2 | 181 | 56 | 231 | 3.62e-72 | 218 |
| MS.gene053537.t1 | AT1G67990 | 54.802 | 177 | 77 | 2 | 5 | 181 | 59 | 232 | 3.00e-66 | 202 |
| MS.gene053537.t1 | AT1G24735 | 54.444 | 180 | 80 | 2 | 2 | 181 | 17 | 194 | 2.90e-65 | 199 |
| MS.gene053537.t1 | AT1G24735 | 54.444 | 180 | 80 | 2 | 2 | 181 | 62 | 239 | 9.61e-65 | 199 |
| MS.gene053537.t1 | AT1G24735 | 54.444 | 180 | 80 | 2 | 2 | 181 | 113 | 290 | 4.45e-64 | 199 |
| MS.gene053537.t1 | AT1G67980 | 45.181 | 166 | 87 | 2 | 16 | 181 | 2 | 163 | 1.89e-48 | 155 |
| MS.gene053537.t1 | AT3G62000 | 42.458 | 179 | 96 | 3 | 2 | 180 | 106 | 277 | 7.58e-42 | 142 |
| MS.gene053537.t1 | AT3G61990 | 42.458 | 179 | 96 | 3 | 2 | 180 | 118 | 289 | 1.05e-39 | 136 |
| MS.gene053537.t1 | AT3G62000 | 40.698 | 172 | 91 | 4 | 2 | 171 | 106 | 268 | 9.89e-33 | 120 |
Find 47 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTAGCATTGATAAGTTTA+AGG | 0.154244 | 4.3:-27251667 | None:intergenic |
| CAAGATCGATTAACCTCTTA+TGG | 0.268687 | 4.3:-27252293 | None:intergenic |
| ATAAATACCTTTCCATCTTC+AGG | 0.276991 | 4.3:-27251749 | None:intergenic |
| GCTAAGAATACCATGGAAAT+TGG | 0.278001 | 4.3:+27251683 | MS.gene053537:CDS |
| TAACAAAGAAAATTACGAAT+TGG | 0.303369 | 4.3:+27252017 | MS.gene053537:CDS |
| TCGGCTACGACAACACCTTA+TGG | 0.326245 | 4.3:+27252334 | MS.gene053537:CDS |
| TACTTCCTCAATGGAGCATC+AGG | 0.355841 | 4.3:-27252378 | None:intergenic |
| TTTGGAGCTTAACAAGGCTT+TGG | 0.370464 | 4.3:+27252425 | MS.gene053537:CDS |
| GTTAATCGATCTTGTTAAAG+TGG | 0.393377 | 4.3:+27252302 | MS.gene053537:CDS |
| AACAAAGAAAATTACGAATT+GGG | 0.394736 | 4.3:+27252018 | MS.gene053537:CDS |
| AGCATACATATTTCAATCCT+AGG | 0.403073 | 4.3:-27252456 | None:intergenic |
| CTCCATTGAGGAAGTATGTT+AGG | 0.425117 | 4.3:+27252385 | MS.gene053537:CDS |
| TTGATCATTTCATCAAGAAC+TGG | 0.432518 | 4.3:-27252105 | None:intergenic |
| GCCCTAGCTATTCCTGAAGA+TGG | 0.453375 | 4.3:+27251737 | MS.gene053537:CDS |
| ATTACCTCAACTACCATAAG+AGG | 0.454646 | 4.3:+27252280 | MS.gene053537:CDS |
| CACAAAATTGATTTCAGAGA+AGG | 0.464239 | 4.3:+27252072 | MS.gene053537:CDS |
| GCTTAACAAGGCTTTGGCTG+TGG | 0.469408 | 4.3:+27252431 | MS.gene053537:CDS |
| AGCTAGGGCAGTGGCAAGGA+GGG | 0.490733 | 4.3:-27251723 | None:intergenic |
| TCATCAAGAACTGGAAGAGC+TGG | 0.494231 | 4.3:-27252096 | None:intergenic |
| AGGCTTTGGCTGTGGACCCT+AGG | 0.500768 | 4.3:+27252439 | MS.gene053537:CDS |
| TACCTAACATACTTCCTCAA+TGG | 0.510094 | 4.3:-27252387 | None:intergenic |
| TTCCATCTTCAGGAATAGCT+AGG | 0.514348 | 4.3:-27251739 | None:intergenic |
| ATAGTGATTCCATCACCAAC+AGG | 0.521355 | 4.3:-27252480 | None:intergenic |
| GTTAAAGTGGGAGGTGTGAT+CGG | 0.548322 | 4.3:+27252315 | MS.gene053537:CDS |
| AACCACAGATCCATTCCATA+AGG | 0.552846 | 4.3:-27252349 | None:intergenic |
| TTAACCTCTTATGGTAGTTG+AGG | 0.561488 | 4.3:-27252284 | None:intergenic |
| TACGACAACACCTTATGGAA+TGG | 0.561597 | 4.3:+27252339 | MS.gene053537:CDS |
| AGCTATTCCTGAAGATGGAA+AGG | 0.565418 | 4.3:+27251742 | MS.gene053537:CDS |
| ATCGATCTTGTTAAAGTGGG+AGG | 0.565493 | 4.3:+27252306 | MS.gene053537:CDS |
| ATGGAAATTGGTGTCTACAC+TGG | 0.567113 | 4.3:+27251695 | MS.gene053537:CDS |
| AAATCAATTACTTGATCCTA+CGG | 0.569584 | 4.3:-27252506 | None:intergenic |
| TCCATCTTCAGGAATAGCTA+GGG | 0.570121 | 4.3:-27251738 | None:intergenic |
| TTAATCGATCTTGTTAAAGT+GGG | 0.572330 | 4.3:+27252303 | MS.gene053537:CDS |
| GAAATATGTATGCTTCCTGT+TGG | 0.574969 | 4.3:+27252465 | MS.gene053537:CDS |
| ACTTCCTCAATGGAGCATCA+GGG | 0.579332 | 4.3:-27252377 | None:intergenic |
| TGTATGCTTCCTGTTGGTGA+TGG | 0.580796 | 4.3:+27252471 | MS.gene053537:CDS |
| TAGCTAGGGCAGTGGCAAGG+AGG | 0.584857 | 4.3:-27251724 | None:intergenic |
| CTTCCTCAATGGAGCATCAG+GGG | 0.588342 | 4.3:-27252376 | None:intergenic |
| GCATACATATTTCAATCCTA+GGG | 0.589825 | 4.3:-27252455 | None:intergenic |
| TTCAGGAATAGCTAGGGCAG+TGG | 0.598779 | 4.3:-27251732 | None:intergenic |
| TGACAACCTCTGCAGATGAA+GGG | 0.611925 | 4.3:+27251626 | None:intergenic |
| AGTGTAGACACCAATTTCCA+TGG | 0.614822 | 4.3:-27251693 | None:intergenic |
| TATCAATGCTAAGAATACCA+TGG | 0.621099 | 4.3:+27251676 | MS.gene053537:CDS |
| CACCTTATGGAATGGATCTG+TGG | 0.632572 | 4.3:+27252347 | MS.gene053537:CDS |
| ATGGAATCACTATCTGCCGT+AGG | 0.633911 | 4.3:+27252490 | MS.gene053537:CDS |
| GAATAGCTAGGGCAGTGGCA+AGG | 0.635775 | 4.3:-27251727 | None:intergenic |
| TTCCTCAATGGAGCATCAGG+GGG | 0.651997 | 4.3:-27252375 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATCTAATTGATTATATAA+TGG | - | chr4.3:27251815-27251834 | None:intergenic | 10.0% |
| !!! | TGATTTTTTGTTAATTTTTT+AGG | + | chr4.3:27252206-27252225 | MS.gene053537:intron | 10.0% |
| !! | AACAAAGAAAATTACGAATT+GGG | + | chr4.3:27252018-27252037 | MS.gene053537:CDS | 20.0% |
| !! | TAACAAAGAAAATTACGAAT+TGG | + | chr4.3:27252017-27252036 | MS.gene053537:CDS | 20.0% |
| !!! | ATGAAAAAAGTTCTAACAAA+AGG | - | chr4.3:27251867-27251886 | None:intergenic | 20.0% |
| !!! | ATTTTTTAGGAAAAGAATCA+TGG | + | chr4.3:27252219-27252238 | MS.gene053537:intron | 20.0% |
| !!! | TTTTTCTTCATAGAAAAAGA+TGG | - | chr4.3:27252145-27252164 | None:intergenic | 20.0% |
| ! | GAGATATCATGTAAAATCAA+GGG | + | chr4.3:27251941-27251960 | MS.gene053537:intron | 25.0% |
| ! | TGAGATATCATGTAAAATCA+AGG | + | chr4.3:27251940-27251959 | MS.gene053537:intron | 25.0% |
| ! | TTAATCGATCTTGTTAAAGT+GGG | + | chr4.3:27252303-27252322 | MS.gene053537:CDS | 25.0% |
| !! | CTTTTGTGTTTAGTAAAGTA+AGG | + | chr4.3:27251888-27251907 | MS.gene053537:intron | 25.0% |
| !! | GGTCTAATACTTAATTTTTC+TGG | + | chr4.3:27251962-27251981 | MS.gene053537:intron | 25.0% |
| !! | GTTGTAATTGTTACAGATTT+TGG | + | chr4.3:27251984-27252003 | MS.gene053537:intron | 25.0% |
| !!! | GTACTATAGAGATTTTGTTT+TGG | + | chr4.3:27252407-27252426 | MS.gene053537:CDS | 25.0% |
| !!! | TCTTAGCATTGATAAGTTTA+AGG | - | chr4.3:27251670-27251689 | None:intergenic | 25.0% |
| AGCATACATATTTCAATCCT+AGG | - | chr4.3:27252459-27252478 | None:intergenic | 30.0% | |
| ATAAATACCTTTCCATCTTC+AGG | - | chr4.3:27251752-27251771 | None:intergenic | 30.0% | |
| CTACCTGTAATTAAAAAAGC+TGG | + | chr4.3:27252042-27252061 | MS.gene053537:CDS | 30.0% | |
| GCATACATATTTCAATCCTA+GGG | - | chr4.3:27252458-27252477 | None:intergenic | 30.0% | |
| GTGTTTAGTAAAGTAAGGTT+AGG | + | chr4.3:27251893-27251912 | MS.gene053537:intron | 30.0% | |
| GTTAATCGATCTTGTTAAAG+TGG | + | chr4.3:27252302-27252321 | MS.gene053537:CDS | 30.0% | |
| TATCAATGCTAAGAATACCA+TGG | + | chr4.3:27251676-27251695 | MS.gene053537:CDS | 30.0% | |
| TTGATCATTTCATCAAGAAC+TGG | - | chr4.3:27252108-27252127 | None:intergenic | 30.0% | |
| ! | CACAAAATTGATTTCAGAGA+AGG | + | chr4.3:27252072-27252091 | MS.gene053537:CDS | 30.0% |
| !! | TAGCTACGATTTCATTTTTG+TGG | + | chr4.3:27252242-27252261 | MS.gene053537:CDS | 30.0% |
| !!! | ACACCAGCTTTTTTAATTAC+AGG | - | chr4.3:27252048-27252067 | None:intergenic | 30.0% |
| !!! | ATTGTTACAGATTTTGGCTA+TGG | + | chr4.3:27251990-27252009 | MS.gene053537:intron | 30.0% |
| !!! | TTTTGTTTTGGAGCTTAACA+AGG | + | chr4.3:27252419-27252438 | MS.gene053537:CDS | 30.0% |
| ATTACCTCAACTACCATAAG+AGG | + | chr4.3:27252280-27252299 | MS.gene053537:CDS | 35.0% | |
| CAAGATCGATTAACCTCTTA+TGG | - | chr4.3:27252296-27252315 | None:intergenic | 35.0% | |
| GAAATATGTATGCTTCCTGT+TGG | + | chr4.3:27252465-27252484 | MS.gene053537:CDS | 35.0% | |
| GCTAAGAATACCATGGAAAT+TGG | + | chr4.3:27251683-27251702 | MS.gene053537:CDS | 35.0% | |
| TACCTAACATACTTCCTCAA+TGG | - | chr4.3:27252390-27252409 | None:intergenic | 35.0% | |
| TTAACCTCTTATGGTAGTTG+AGG | - | chr4.3:27252287-27252306 | None:intergenic | 35.0% | |
| AACCACAGATCCATTCCATA+AGG | - | chr4.3:27252352-27252371 | None:intergenic | 40.0% | |
| AGCTATTCCTGAAGATGGAA+AGG | + | chr4.3:27251742-27251761 | MS.gene053537:CDS | 40.0% | |
| AGTGTAGACACCAATTTCCA+TGG | - | chr4.3:27251696-27251715 | None:intergenic | 40.0% | |
| ATAGTGATTCCATCACCAAC+AGG | - | chr4.3:27252483-27252502 | None:intergenic | 40.0% | |
| ATCGATCTTGTTAAAGTGGG+AGG | + | chr4.3:27252306-27252325 | MS.gene053537:CDS | 40.0% | |
| TACGACAACACCTTATGGAA+TGG | + | chr4.3:27252339-27252358 | MS.gene053537:CDS | 40.0% | |
| TCCATCTTCAGGAATAGCTA+GGG | - | chr4.3:27251741-27251760 | None:intergenic | 40.0% | |
| TTCCATCTTCAGGAATAGCT+AGG | - | chr4.3:27251742-27251761 | None:intergenic | 40.0% | |
| ! | ATGGAAATTGGTGTCTACAC+TGG | + | chr4.3:27251695-27251714 | MS.gene053537:CDS | 40.0% |
| ! | CTCCATTGAGGAAGTATGTT+AGG | + | chr4.3:27252385-27252404 | MS.gene053537:CDS | 40.0% |
| ! | TTTGGAGCTTAACAAGGCTT+TGG | + | chr4.3:27252425-27252444 | MS.gene053537:CDS | 40.0% |
| ATGGAATCACTATCTGCCGT+AGG | + | chr4.3:27252490-27252509 | MS.gene053537:CDS | 45.0% | |
| CACCTTATGGAATGGATCTG+TGG | + | chr4.3:27252347-27252366 | MS.gene053537:CDS | 45.0% | |
| TCATCAAGAACTGGAAGAGC+TGG | - | chr4.3:27252099-27252118 | None:intergenic | 45.0% | |
| ! | ACTTCCTCAATGGAGCATCA+GGG | - | chr4.3:27252380-27252399 | None:intergenic | 45.0% |
| ! | GTTAAAGTGGGAGGTGTGAT+CGG | + | chr4.3:27252315-27252334 | MS.gene053537:CDS | 45.0% |
| ! | TACTTCCTCAATGGAGCATC+AGG | - | chr4.3:27252381-27252400 | None:intergenic | 45.0% |
| ! | TGTATGCTTCCTGTTGGTGA+TGG | + | chr4.3:27252471-27252490 | MS.gene053537:CDS | 45.0% |
| GCCCTAGCTATTCCTGAAGA+TGG | + | chr4.3:27251737-27251756 | MS.gene053537:CDS | 50.0% | |
| TCGGCTACGACAACACCTTA+TGG | + | chr4.3:27252334-27252353 | MS.gene053537:CDS | 50.0% | |
| TTCAGGAATAGCTAGGGCAG+TGG | - | chr4.3:27251735-27251754 | None:intergenic | 50.0% | |
| ! | CTTCCTCAATGGAGCATCAG+GGG | - | chr4.3:27252379-27252398 | None:intergenic | 50.0% |
| ! | GCTTAACAAGGCTTTGGCTG+TGG | + | chr4.3:27252431-27252450 | MS.gene053537:CDS | 50.0% |
| ! | TTCCTCAATGGAGCATCAGG+GGG | - | chr4.3:27252378-27252397 | None:intergenic | 50.0% |
| GAATAGCTAGGGCAGTGGCA+AGG | - | chr4.3:27251730-27251749 | None:intergenic | 55.0% | |
| AGCTAGGGCAGTGGCAAGGA+GGG | - | chr4.3:27251726-27251745 | None:intergenic | 60.0% | |
| CACCCCCTGATGCTCCATTG+AGG | + | chr4.3:27252373-27252392 | MS.gene053537:CDS | 60.0% | |
| TAGCTAGGGCAGTGGCAAGG+AGG | - | chr4.3:27251727-27251746 | None:intergenic | 60.0% | |
| ! | AGGCTTTGGCTGTGGACCCT+AGG | + | chr4.3:27252439-27252458 | MS.gene053537:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 27251641 | 27252521 | 27251641 | ID=MS.gene053537 |
| chr4.3 | mRNA | 27251641 | 27252521 | 27251641 | ID=MS.gene053537.t1;Parent=MS.gene053537 |
| chr4.3 | exon | 27251641 | 27251763 | 27251641 | ID=MS.gene053537.t1.exon1;Parent=MS.gene053537.t1 |
| chr4.3 | CDS | 27251641 | 27251763 | 27251641 | ID=cds.MS.gene053537.t1;Parent=MS.gene053537.t1 |
| chr4.3 | exon | 27252000 | 27252131 | 27252000 | ID=MS.gene053537.t1.exon2;Parent=MS.gene053537.t1 |
| chr4.3 | CDS | 27252000 | 27252131 | 27252000 | ID=cds.MS.gene053537.t1;Parent=MS.gene053537.t1 |
| chr4.3 | exon | 27252228 | 27252521 | 27252228 | ID=MS.gene053537.t1.exon3;Parent=MS.gene053537.t1 |
| chr4.3 | CDS | 27252228 | 27252521 | 27252228 | ID=cds.MS.gene053537.t1;Parent=MS.gene053537.t1 |
| Gene Sequence |
| Protein sequence |
