AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053631

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053631.t1 MTR_4g092700 95.806 310 13 0 1 310 24 333 0.0 618
MS.gene053631.t1 MTR_2g010450 53.582 349 118 9 4 310 7 353 6.49e-112 328
MS.gene053631.t1 MTR_5g014600 47.347 245 94 7 88 306 98 333 1.53e-57 188
MS.gene053631.t1 MTR_4g097940 38.209 335 175 9 1 310 1 328 4.64e-56 184
MS.gene053631.t1 MTR_4g097920 37.612 335 177 9 1 310 1 328 1.13e-53 178
MS.gene053631.t1 MTR_4g097920 42.912 261 138 6 54 310 60 313 1.28e-52 175
MS.gene053631.t1 MTR_5g014520 49.198 187 93 2 118 303 140 325 6.04e-51 171
MS.gene053631.t1 MTR_4g097950 48.673 226 89 9 94 303 31 245 2.44e-45 154
MS.gene053631.t1 MTR_5g014640 32.927 328 178 11 1 306 15 322 2.30e-44 154
MS.gene053631.t1 MTR_8g009020 38.095 252 121 6 72 303 82 318 4.76e-44 153
MS.gene053631.t1 MTR_0250s0040 44.809 183 89 3 134 310 87 263 6.89e-44 151
MS.gene053631.t1 MTR_2g104550 46.857 175 88 2 135 306 138 310 1.20e-42 149
MS.gene053631.t1 MTR_6g047570 46.784 171 89 2 134 303 158 327 1.86e-42 149
MS.gene053631.t1 MTR_0246s0020 44.724 199 100 5 119 310 182 377 3.68e-42 150
MS.gene053631.t1 MTR_5g014560 43.931 173 95 2 134 305 130 301 2.53e-41 145
MS.gene053631.t1 MTR_0011s0210 41.808 177 103 0 134 310 182 358 7.25e-40 143
MS.gene053631.t1 MTR_0011s0260 43.529 170 96 0 134 303 182 351 1.28e-39 142
MS.gene053631.t1 MTR_4g009540 43.820 178 94 1 135 306 152 329 1.86e-39 141
MS.gene053631.t1 MTR_4g066460 40.000 215 107 5 107 309 122 326 2.18e-39 141
MS.gene053631.t1 MTR_4g009110 44.571 175 94 2 135 306 174 348 5.27e-38 138
MS.gene053631.t1 MTR_8g009010 41.011 178 68 2 134 310 133 274 2.65e-37 135
MS.gene053631.t1 MTR_0246s0020 46.591 176 84 5 119 287 182 354 4.71e-37 136
MS.gene053631.t1 MTR_4g067010 40.741 189 98 4 127 310 140 319 6.30e-37 134
MS.gene053631.t1 MTR_2g104490 43.103 174 97 1 135 308 137 308 1.60e-36 133
MS.gene053631.t1 MTR_4g066380 37.915 211 118 4 107 310 120 324 2.91e-36 133
MS.gene053631.t1 MTR_7g080780 43.158 190 96 5 115 303 133 311 5.44e-36 132
MS.gene053631.t1 MTR_7g080780 43.158 190 96 5 115 303 146 324 6.49e-36 132
MS.gene053631.t1 MTR_0246s0050 40.449 178 97 3 134 310 150 319 2.38e-35 130
MS.gene053631.t1 MTR_4g098035 40.449 178 97 3 134 310 171 340 2.81e-35 130
MS.gene053631.t1 MTR_2g104560 38.636 176 94 4 134 303 53 220 3.25e-34 125
MS.gene053631.t1 MTR_4g009540 44.025 159 83 1 135 287 152 310 5.18e-34 126
MS.gene053631.t1 MTR_2g104490 41.615 161 92 1 135 295 137 295 1.32e-32 122
MS.gene053631.t1 MTR_2g104590 38.418 177 83 4 135 307 69 223 3.46e-30 114
MS.gene053631.t1 MTR_2g104530 35.838 173 107 2 136 308 106 274 5.25e-30 115
MS.gene053631.t1 MTR_8g069740 38.674 181 101 4 110 286 91 265 1.64e-29 113
MS.gene053631.t1 MTR_2g104650 38.119 202 115 5 110 307 73 268 2.44e-28 110
MS.gene053631.t1 MTR_2g104500 34.911 169 105 1 135 303 104 267 8.63e-28 109
MS.gene053631.t1 MTR_4g009510 37.430 179 94 5 133 308 42 205 5.37e-24 97.4
MS.gene053631.t1 MTR_7g451650 36.601 153 87 2 156 308 55 197 7.63e-24 96.7
MS.gene053631.t1 MTR_5g014550 41.732 127 72 2 165 290 54 179 3.99e-23 94.7
MS.gene053631.t1 MTR_4g009420 35.294 153 81 2 156 308 63 197 8.18e-22 91.3
MS.gene053631.t1 MTR_7g050350 36.054 147 80 2 164 308 1 135 1.82e-21 88.6
MS.gene053631.t1 MTR_7g451650 36.054 147 80 2 164 308 1 135 1.82e-21 88.6
MS.gene053631.t1 MTR_6g047550 64.286 70 24 1 134 203 172 240 5.31e-21 90.9
MS.gene053631.t1 MTR_7g451650 36.765 136 76 2 156 291 55 180 3.07e-20 87.0
MS.gene053631.t1 MTR_4g009420 35.075 134 69 2 158 291 65 180 4.93e-18 81.3
MS.gene053631.t1 MTR_8g027495 38.053 113 62 3 133 245 456 560 3.84e-16 79.3
MS.gene053631.t1 MTR_7g096350 53.125 64 30 0 133 196 353 416 4.05e-16 79.0
MS.gene053631.t1 MTR_1g072320 29.310 174 113 3 103 266 436 609 7.12e-16 78.6
MS.gene053631.t1 MTR_7g117670 34.058 138 81 3 133 269 400 528 7.27e-16 78.2
MS.gene053631.t1 MTR_8g067280 30.380 158 81 3 133 270 460 608 6.55e-15 75.5
MS.gene053631.t1 MTR_8g024790 29.091 165 98 4 120 273 274 430 9.05e-15 74.7
MS.gene053631.t1 MTR_3g498695 28.221 163 107 3 129 281 292 454 2.96e-14 73.2
MS.gene053631.t1 MTR_3g498695 28.221 163 107 3 129 281 292 454 3.04e-14 73.2
MS.gene053631.t1 MTR_3g030700 26.804 194 115 4 106 286 173 352 8.20e-14 71.6
MS.gene053631.t1 MTR_7g083900 29.012 162 103 4 132 281 283 444 3.14e-13 70.1
MS.gene053631.t1 MTR_7g083900 29.012 162 103 4 132 281 283 444 3.50e-13 70.1
MS.gene053631.t1 MTR_5g030430 28.188 149 96 2 133 270 491 639 3.87e-13 70.1
MS.gene053631.t1 MTR_8g098275 40.476 84 45 1 113 191 217 300 4.60e-13 69.7
MS.gene053631.t1 MTR_8g098275 46.269 67 36 0 125 191 298 364 5.98e-13 69.3
MS.gene053631.t1 MTR_8g064700 30.968 155 98 3 137 284 246 398 6.88e-13 68.9
MS.gene053631.t1 MTR_8g098275 46.269 67 36 0 125 191 391 457 7.29e-13 69.3
MS.gene053631.t1 MTR_3g104100 26.627 169 94 4 133 281 193 351 2.59e-12 67.0
MS.gene053631.t1 MTR_8g024770 26.630 184 117 4 120 291 262 439 5.10e-12 66.6
MS.gene053631.t1 MTR_2g038040 26.829 164 103 4 136 284 53 214 6.49e-12 64.7
MS.gene053631.t1 MTR_2g084280 30.216 139 91 4 137 273 135 269 7.87e-12 65.5
MS.gene053631.t1 MTR_1g112680 49.091 55 28 0 136 190 318 372 2.51e-11 64.7
MS.gene053631.t1 MTR_2g081040 26.627 169 94 4 133 281 175 333 4.06e-11 63.5
MS.gene053631.t1 MTR_4g057270 27.711 166 103 5 137 297 151 304 7.91e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053631.t1 AT4G37850 55.556 180 75 3 134 310 151 328 3.27e-55 182
MS.gene053631.t1 AT4G37850 55.556 180 75 3 134 310 151 328 3.27e-55 182
MS.gene053631.t1 AT2G22760 47.727 176 88 2 132 303 116 291 2.24e-48 164
MS.gene053631.t1 AT2G22750 50.000 214 93 7 96 302 91 297 1.37e-44 154
MS.gene053631.t1 AT2G22750 50.000 214 93 7 96 302 119 325 1.87e-44 154
MS.gene053631.t1 AT2G22750 50.000 214 93 7 96 302 115 321 2.03e-44 154
MS.gene053631.t1 AT2G22750 50.000 214 92 8 96 302 91 296 4.13e-43 150
MS.gene053631.t1 AT2G22750 50.000 214 92 8 96 302 119 324 5.33e-43 150
MS.gene053631.t1 AT2G22770 43.850 187 90 2 132 303 129 315 6.11e-41 145
MS.gene053631.t1 AT2G22760 45.283 159 84 1 132 287 116 274 2.62e-40 142
MS.gene053631.t1 AT2G46510 41.441 111 47 1 133 243 393 485 4.54e-18 85.1
MS.gene053631.t1 AT1G32640 35.664 143 85 2 133 271 450 589 2.24e-16 80.1
MS.gene053631.t1 AT1G01260 45.455 77 40 1 117 191 413 489 2.29e-14 73.9
MS.gene053631.t1 AT1G01260 45.455 77 40 1 117 191 413 489 2.29e-14 73.9
MS.gene053631.t1 AT1G01260 45.455 77 40 1 117 191 413 489 2.29e-14 73.9
MS.gene053631.t1 AT4G17880 30.556 144 90 3 133 271 414 552 4.16e-14 73.2
MS.gene053631.t1 AT2G16910 29.897 194 100 7 136 301 315 500 4.91e-14 72.8
MS.gene053631.t1 AT5G46760 31.469 143 94 2 133 271 413 555 5.10e-14 72.8
MS.gene053631.t1 AT4G29930 30.357 168 103 5 124 290 46 200 5.51e-14 70.5
MS.gene053631.t1 AT3G26744 29.375 160 103 3 132 281 304 463 5.93e-14 72.4
MS.gene053631.t1 AT3G26744 29.375 160 103 3 132 281 304 463 6.76e-14 72.4
MS.gene053631.t1 AT3G26744 29.375 160 103 3 132 281 304 463 6.76e-14 72.4
MS.gene053631.t1 AT3G26744 29.375 160 103 3 132 281 304 463 6.76e-14 72.4
MS.gene053631.t1 AT4G29930 28.492 179 95 5 124 290 46 203 6.94e-14 70.1
MS.gene053631.t1 AT4G16430 48.438 64 33 0 135 198 320 383 1.54e-13 71.2
MS.gene053631.t1 AT4G29930 29.697 165 102 5 124 287 46 197 3.57e-13 68.2
MS.gene053631.t1 AT1G12860 29.114 158 102 5 132 281 264 419 4.83e-13 69.7
MS.gene053631.t1 AT5G57150 31.410 156 84 4 136 275 55 203 4.84e-13 67.8
MS.gene053631.t1 AT5G57150 31.410 156 84 4 136 275 56 204 5.36e-13 67.4
MS.gene053631.t1 AT5G57150 31.410 156 84 4 136 275 56 204 5.77e-13 67.4
MS.gene053631.t1 AT4G09820 30.282 142 70 4 132 267 357 475 6.23e-13 69.3
MS.gene053631.t1 AT1G12860 29.114 158 102 5 132 281 264 419 6.43e-13 69.3
MS.gene053631.t1 AT5G57150 30.769 156 85 3 136 275 55 203 1.01e-12 67.4
MS.gene053631.t1 AT1G63650 38.554 83 51 0 135 217 405 487 1.09e-12 68.9
MS.gene053631.t1 AT1G63650 38.554 83 51 0 135 217 405 487 1.09e-12 68.9
MS.gene053631.t1 AT1G63650 38.554 83 51 0 135 217 405 487 1.09e-12 68.9
MS.gene053631.t1 AT5G57150 31.410 156 84 4 136 275 56 204 1.40e-12 67.0
MS.gene053631.t1 AT4G29930 28.191 188 110 6 124 290 46 229 6.35e-12 65.1
MS.gene053631.t1 AT5G41315 35.789 95 53 2 102 188 405 499 4.05e-11 64.3
MS.gene053631.t1 AT5G41315 35.789 95 53 2 102 188 408 502 4.41e-11 63.9
MS.gene053631.t1 AT5G41315 35.789 95 53 2 102 188 400 494 4.50e-11 63.9
MS.gene053631.t1 AT4G29930 44.776 67 34 1 124 190 46 109 4.60e-11 61.2
MS.gene053631.t1 AT4G29930 27.568 185 109 7 124 287 46 226 4.84e-11 62.4
MS.gene053631.t1 AT4G29930 27.568 185 109 7 124 287 46 226 4.84e-11 62.4
MS.gene053631.t1 AT3G61950 30.556 144 92 4 135 273 128 268 6.87e-11 62.4

Find 55 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCTTGCTTCAATTTC+TGG 0.046048 4.4:-21737264 None:intergenic
GCAGTTTCCATTGAAGTTCT+AGG 0.252953 4.4:-21736480 None:intergenic
ATGGACAAAGTTACTGTTCT+TGG 0.276006 4.4:+21737072 MS.gene053631:CDS
AATAGCAGTTTCATGACATT+TGG 0.296146 4.4:+21737384 MS.gene053631:CDS
GCTTTCTTTCAGATATTATA+TGG 0.308934 4.4:-21736710 None:intergenic
CTGCCAATAGGTACTGCTTT+TGG 0.314519 4.4:+21736414 MS.gene053631:CDS
GCTCTATCTTCCCTTGTTCC+CGG 0.327620 4.4:+21736762 MS.gene053631:CDS
GTGCAATTTGCAGATGGATA+TGG 0.328392 4.4:+21737615 MS.gene053631:intron
GATTGAGTTTCTCAATTTCA+TGG 0.328907 4.4:-21737350 None:intergenic
GAAAATTCTTCTGCAGAATC+TGG 0.357591 4.4:+21737219 MS.gene053631:CDS
AGGAACCTTTGAGGCCAAAA+AGG 0.379217 4.4:+21736653 MS.gene053631:CDS
TCGACAAATGAAAGAAGATT+CGG 0.392423 4.4:-21736534 None:intergenic
GAAGTTCTAGGGTTGAAGTT+TGG 0.405401 4.4:-21736468 None:intergenic
TATCAGAGGGTTTCCTGAGC+AGG 0.415708 4.4:+21736206 MS.gene053631:CDS
ATAGCAGTTTCATGACATTT+GGG 0.420095 4.4:+21737385 MS.gene053631:CDS
TTTGAATCAGTTAAATCAGT+TGG 0.433019 4.4:+21736557 MS.gene053631:CDS
CCTTGTTCCCGGACTACAAA+AGG 0.436297 4.4:+21736773 MS.gene053631:CDS
TCTCACAGTGAACTCTTATG+AGG 0.440028 4.4:-21737299 None:intergenic
TCTTGAGGAAGAGAGAGTTT+AGG 0.440922 4.4:-21736687 None:intergenic
CTTCAACCCTAGAACTTCAA+TGG 0.444938 4.4:+21736473 MS.gene053631:CDS
CAGTTTCCATTGAAGTTCTA+GGG 0.451054 4.4:-21736479 None:intergenic
TTGAATCAGTTAAATCAGTT+GGG 0.451883 4.4:+21736558 MS.gene053631:CDS
ACTATATATCTTACCTGCTC+AGG 0.457618 4.4:-21736219 None:intergenic
GAGTCTAACTCACTGCCAAT+AGG 0.462111 4.4:+21736402 MS.gene053631:CDS
GAACCAATCATCTCATCCTT+AGG 0.467424 4.4:-21736591 None:intergenic
TCTCCAAAAGCAGTACCTAT+TGG 0.467684 4.4:-21736417 None:intergenic
GAGAATATTTCATTTATCAG+AGG 0.468225 4.4:+21736192 MS.gene053631:CDS
CGACAAATGAAAGAAGATTC+GGG 0.471965 4.4:-21736533 None:intergenic
TTTGATCTTGCAGGAAATGA+TGG 0.481839 4.4:+21736332 MS.gene053631:intron
GATTTCTCAAGGAACCTTTG+AGG 0.485480 4.4:+21736644 MS.gene053631:CDS
AAATGTCATGAAACTGCTAT+TGG 0.489746 4.4:-21737382 None:intergenic
TTCTGGCAGTTCCTCGTCGA+AGG 0.494903 4.4:-21737247 None:intergenic
CTTCATTTCTTCATCATCAG+TGG 0.507118 4.4:+21736361 MS.gene053631:CDS
AAGCCTAAGGATGAGATGAT+TGG 0.509779 4.4:+21736588 MS.gene053631:CDS
TGATATAACAATTATTGCAC+AGG 0.531379 4.4:+21737419 MS.gene053631:CDS
AGAATATTTCATTTATCAGA+GGG 0.537343 4.4:+21736193 MS.gene053631:CDS
TGAGAAAATCAAAGGAGTTG+TGG 0.539482 4.4:+21737317 MS.gene053631:CDS
CACTGATGATGAAGAAATGA+AGG 0.551145 4.4:-21736360 None:intergenic
TCTTCCGACATGATTTCTCA+AGG 0.551521 4.4:+21736633 MS.gene053631:CDS
TCAGATATTATATGGTCTTG+AGG 0.555208 4.4:-21736702 None:intergenic
AAGATAGAGCAATGAAGCGC+TGG 0.560225 4.4:-21736749 None:intergenic
GTTCACTGTGAGAAAATCAA+AGG 0.561408 4.4:+21737309 MS.gene053631:CDS
AGTTTGGATAAACGTATGAA+TGG 0.568981 4.4:-21736452 None:intergenic
AGAGAAGGTGAAGGTTCTTG+AGG 0.571434 4.4:+21737125 MS.gene053631:CDS
TCTGGCAGTTCCTCGTCGAA+GGG 0.571994 4.4:-21737246 None:intergenic
CTTGAAGAAATTGCAAGAGA+AGG 0.585063 4.4:+21737110 MS.gene053631:CDS
GTTGGGATTAGTGAAGCCTA+AGG 0.603362 4.4:+21736575 MS.gene053631:CDS
GAAGGTGAAGGTTCTTGAGG+AGG 0.607165 4.4:+21737128 MS.gene053631:CDS
ATATAATATCTGAAAGAAAG+CGG 0.617975 4.4:+21736712 MS.gene053631:CDS
AATGAAAGAAGATTCGGGTA+CGG 0.630607 4.4:-21736528 None:intergenic
GATCTTGCAGGAAATGATGG+AGG 0.636854 4.4:+21736335 MS.gene053631:intron
GAAATTGCAAGAGAAGGTGA+AGG 0.642638 4.4:+21737116 MS.gene053631:CDS
AATTCTTCTGCAGAATCTGG+TGG 0.642920 4.4:+21737222 MS.gene053631:CDS
ATCTGGTGGTCCCTTCGACG+AGG 0.665673 4.4:+21737236 MS.gene053631:CDS
GGTTCCTTGAGAAATCATGT+CGG 0.702500 4.4:-21736637 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAAGAATGTGTTTG+TGG + chr4.4:21736995-21737014 MS.gene053631:intron 20.0%
!! ACAAATTTGACAAATTTGTA+AGG + chr4.4:21736855-21736874 MS.gene053631:intron 20.0%
!! AGAATATTTCATTTATCAGA+GGG + chr4.4:21736193-21736212 MS.gene053631:CDS 20.0%
!! ATATAATATCTGAAAGAAAG+CGG + chr4.4:21736712-21736731 MS.gene053631:CDS 20.0%
!! ATTTAGTGTTTACTATTAGT+AGG + chr4.4:21736962-21736981 MS.gene053631:intron 20.0%
! ACTACAATTGTCTATCAATA+TGG + chr4.4:21737583-21737602 MS.gene053631:intron 25.0%
! CTTACTATACATTCATACTT+TGG + chr4.4:21736266-21736285 MS.gene053631:intron 25.0%
! GAGAATATTTCATTTATCAG+AGG + chr4.4:21736192-21736211 MS.gene053631:CDS 25.0%
! GCTTTCTTTCAGATATTATA+TGG - chr4.4:21736713-21736732 None:intergenic 25.0%
! TGATATAACAATTATTGCAC+AGG + chr4.4:21737419-21737438 MS.gene053631:CDS 25.0%
! TTGAATCAGTTAAATCAGTT+GGG + chr4.4:21736558-21736577 MS.gene053631:CDS 25.0%
! TTTGAATCAGTTAAATCAGT+TGG + chr4.4:21736557-21736576 MS.gene053631:CDS 25.0%
!! AGAAGAATTTTCAACATCAT+TGG - chr4.4:21737211-21737230 None:intergenic 25.0%
!! TTTTTATACCTTTTGTAGTC+CGG - chr4.4:21736784-21736803 None:intergenic 25.0%
AAATGTCATGAAACTGCTAT+TGG - chr4.4:21737385-21737404 None:intergenic 30.0%
AATAGCAGTTTCATGACATT+TGG + chr4.4:21737384-21737403 MS.gene053631:CDS 30.0%
AGTTTGGATAAACGTATGAA+TGG - chr4.4:21736455-21736474 None:intergenic 30.0%
ATAGCAGTTTCATGACATTT+GGG + chr4.4:21737385-21737404 MS.gene053631:CDS 30.0%
CAAAATCTTGCTTCAATTTC+TGG - chr4.4:21737267-21737286 None:intergenic 30.0%
GACAATTGTAGTAATTTCGT+TGG - chr4.4:21737575-21737594 None:intergenic 30.0%
GATTGAGTTTCTCAATTTCA+TGG - chr4.4:21737353-21737372 None:intergenic 30.0%
TATCTATGTTGAACACATGT+TGG + chr4.4:21736936-21736955 MS.gene053631:intron 30.0%
TCGACAAATGAAAGAAGATT+CGG - chr4.4:21736537-21736556 None:intergenic 30.0%
TTTACTATTAGTAGGACACA+CGG + chr4.4:21736970-21736989 MS.gene053631:intron 30.0%
! CATGTTTTCACAATGTTAGA+TGG - chr4.4:21736310-21736329 None:intergenic 30.0%
! TCAGATATTATATGGTCTTG+AGG - chr4.4:21736705-21736724 None:intergenic 30.0%
! TTTTATACCTTTTGTAGTCC+GGG - chr4.4:21736783-21736802 None:intergenic 30.0%
AAACATGTGTTTGATCTTGC+AGG + chr4.4:21736323-21736342 MS.gene053631:intron 35.0%
AATGAAAGAAGATTCGGGTA+CGG - chr4.4:21736531-21736550 None:intergenic 35.0%
ACAGAACATGAAGAAAAACG+TGG + chr4.4:21737152-21737171 MS.gene053631:CDS 35.0%
ACTATATATCTTACCTGCTC+AGG - chr4.4:21736222-21736241 None:intergenic 35.0%
ATGGACAAAGTTACTGTTCT+TGG + chr4.4:21737072-21737091 MS.gene053631:CDS 35.0%
CAAATTTGTAGAGTTGAGTC+AGG - chr4.4:21736844-21736863 None:intergenic 35.0%
CAGTTTCCATTGAAGTTCTA+GGG - chr4.4:21736482-21736501 None:intergenic 35.0%
CGACAAATGAAAGAAGATTC+GGG - chr4.4:21736536-21736555 None:intergenic 35.0%
CTATTTGTGCAATTTGCAGA+TGG + chr4.4:21737609-21737628 MS.gene053631:intron 35.0%
CTTCATTTCTTCATCATCAG+TGG + chr4.4:21736361-21736380 MS.gene053631:CDS 35.0%
CTTGAAGAAATTGCAAGAGA+AGG + chr4.4:21737110-21737129 MS.gene053631:CDS 35.0%
GAAAATTCTTCTGCAGAATC+TGG + chr4.4:21737219-21737238 MS.gene053631:CDS 35.0%
GAAGAAAAACGTGGAATTTG+TGG + chr4.4:21737161-21737180 MS.gene053631:CDS 35.0%
GTTCACTGTGAGAAAATCAA+AGG + chr4.4:21737309-21737328 MS.gene053631:CDS 35.0%
TGAGAAAATCAAAGGAGTTG+TGG + chr4.4:21737317-21737336 MS.gene053631:CDS 35.0%
TTGTAAGGTGAAGATTGCAT+AGG + chr4.4:21736870-21736889 MS.gene053631:intron 35.0%
TTTGATCTTGCAGGAAATGA+TGG + chr4.4:21736332-21736351 MS.gene053631:intron 35.0%
! ACTTTGGCAGTGACTAATTT+TGG + chr4.4:21736282-21736301 MS.gene053631:intron 35.0%
! ATTGTGCTACTACTAACAGA+TGG + chr4.4:21737053-21737072 MS.gene053631:intron 35.0%
! CACTGATGATGAAGAAATGA+AGG - chr4.4:21736363-21736382 None:intergenic 35.0%
! GGATATGGAATTTTGCATGA+CGG + chr4.4:21737630-21737649 MS.gene053631:CDS 35.0%
AAAAACTTAAGTCCCGCGTT+AGG - chr4.4:21736910-21736929 None:intergenic 40.0%
AAGACGTAAAAACTGAGCGT+AGG - chr4.4:21737675-21737694 None:intergenic 40.0%
AAGCCTAAGGATGAGATGAT+TGG + chr4.4:21736588-21736607 MS.gene053631:CDS 40.0%
AATTCTTCTGCAGAATCTGG+TGG + chr4.4:21737222-21737241 MS.gene053631:CDS 40.0%
CTTCAACCCTAGAACTTCAA+TGG + chr4.4:21736473-21736492 MS.gene053631:CDS 40.0%
GAAAAACGTGGAATTTGTGG+TGG + chr4.4:21737164-21737183 MS.gene053631:CDS 40.0%
GAAATTGCAAGAGAAGGTGA+AGG + chr4.4:21737116-21737135 MS.gene053631:CDS 40.0%
GAACCAATCATCTCATCCTT+AGG - chr4.4:21736594-21736613 None:intergenic 40.0%
GATTTCTCAAGGAACCTTTG+AGG + chr4.4:21736644-21736663 MS.gene053631:CDS 40.0%
GCAGTTTCCATTGAAGTTCT+AGG - chr4.4:21736483-21736502 None:intergenic 40.0%
GGTTCCTTGAGAAATCATGT+CGG - chr4.4:21736640-21736659 None:intergenic 40.0%
GTGCAATTTGCAGATGGATA+TGG + chr4.4:21737615-21737634 MS.gene053631:intron 40.0%
TCTCACAGTGAACTCTTATG+AGG - chr4.4:21737302-21737321 None:intergenic 40.0%
TCTCCAAAAGCAGTACCTAT+TGG - chr4.4:21736420-21736439 None:intergenic 40.0%
TCTTCCGACATGATTTCTCA+AGG + chr4.4:21736633-21736652 MS.gene053631:CDS 40.0%
TCTTGAGGAAGAGAGAGTTT+AGG - chr4.4:21736690-21736709 None:intergenic 40.0%
TTTATGATCTCTGCCTAACG+CGG + chr4.4:21736894-21736913 MS.gene053631:intron 40.0%
!! GAAGTTCTAGGGTTGAAGTT+TGG - chr4.4:21736471-21736490 None:intergenic 40.0%
AAGATAGAGCAATGAAGCGC+TGG - chr4.4:21736752-21736771 None:intergenic 45.0%
AGAGAAGGTGAAGGTTCTTG+AGG + chr4.4:21737125-21737144 MS.gene053631:CDS 45.0%
GAGTCTAACTCACTGCCAAT+AGG + chr4.4:21736402-21736421 MS.gene053631:CDS 45.0%
GATCTTGCAGGAAATGATGG+AGG + chr4.4:21736335-21736354 MS.gene053631:intron 45.0%
TTATGATCTCTGCCTAACGC+GGG + chr4.4:21736895-21736914 MS.gene053631:intron 45.0%
! CTTTTGTAGTCCGGGAACAA+GGG - chr4.4:21736775-21736794 None:intergenic 45.0%
! GGAATTTTGCATGACGGTGA+AGG + chr4.4:21737636-21737655 MS.gene053631:CDS 45.0%
! GTTGGGATTAGTGAAGCCTA+AGG + chr4.4:21736575-21736594 MS.gene053631:CDS 45.0%
!! AGGAACCTTTGAGGCCAAAA+AGG + chr4.4:21736653-21736672 MS.gene053631:CDS 45.0%
!! AGGACGTGTTGCTACCTTTT+TGG - chr4.4:21736670-21736689 None:intergenic 45.0%
!! CTGCCAATAGGTACTGCTTT+TGG + chr4.4:21736414-21736433 MS.gene053631:CDS 45.0%
!! TGCTACCTTTTTGGCCTCAA+AGG - chr4.4:21736661-21736680 None:intergenic 45.0%
CCTTGTTCCCGGACTACAAA+AGG + chr4.4:21736773-21736792 MS.gene053631:CDS 50.0%
GAAGGTGAAGGTTCTTGAGG+AGG + chr4.4:21737128-21737147 MS.gene053631:CDS 50.0%
GCTCTATCTTCCCTTGTTCC+CGG + chr4.4:21736762-21736781 MS.gene053631:CDS 50.0%
TATCAGAGGGTTTCCTGAGC+AGG + chr4.4:21736206-21736225 MS.gene053631:CDS 50.0%
! CCTTTTGTAGTCCGGGAACA+AGG - chr4.4:21736776-21736795 None:intergenic 50.0%
TCTGGCAGTTCCTCGTCGAA+GGG - chr4.4:21737249-21737268 None:intergenic 55.0%
TTCTGGCAGTTCCTCGTCGA+AGG - chr4.4:21737250-21737269 None:intergenic 55.0%
ATCTGGTGGTCCCTTCGACG+AGG + chr4.4:21737236-21737255 MS.gene053631:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 21736189 21737702 21736189 ID=MS.gene053631
chr4.4 mRNA 21736189 21737702 21736189 ID=MS.gene053631.t1;Parent=MS.gene053631
chr4.4 exon 21736189 21736227 21736189 ID=MS.gene053631.t1.exon1;Parent=MS.gene053631.t1
chr4.4 CDS 21736189 21736227 21736189 ID=cds.MS.gene053631.t1;Parent=MS.gene053631.t1
chr4.4 exon 21736345 21736794 21736345 ID=MS.gene053631.t1.exon2;Parent=MS.gene053631.t1
chr4.4 CDS 21736345 21736794 21736345 ID=cds.MS.gene053631.t1;Parent=MS.gene053631.t1
chr4.4 exon 21737072 21737440 21737072 ID=MS.gene053631.t1.exon3;Parent=MS.gene053631.t1
chr4.4 CDS 21737072 21737440 21737072 ID=cds.MS.gene053631.t1;Parent=MS.gene053631.t1
chr4.4 exon 21737628 21737702 21737628 ID=MS.gene053631.t1.exon4;Parent=MS.gene053631.t1
chr4.4 CDS 21737628 21737702 21737628 ID=cds.MS.gene053631.t1;Parent=MS.gene053631.t1
Gene Sequence

>MS.gene053631

ATGGAGAATATTTCATTTATCAGAGGGTTTCCTGAGCAGGTAAGATATATAGTTCTTGCAAATAGTTTTTCTCTGAACTTACTATACATTCATACTTTGGCAGTGACTAATTTTGGAACCATCTAACATTGTGAAAACATGTGTTTGATCTTGCAGGAAATGATGGAGGATCCTTCATTTCTTCATCATCAGTGGCATTTGAGCTCTATCAATGAGTCTAACTCACTGCCAATAGGTACTGCTTTTGGAGACACTTCACAACACCATTCATACGTTTATCCAAACTTCAACCCTAGAACTTCAATGGAAACTGCTCAGACACTAGAGACTCAATTTGTTCCGTACCCGAATCTTCTTTCATTTGTCGATTTGAATCAGTTAAATCAGTTGGGATTAGTGAAGCCTAAGGATGAGATGATTGGTTCTCAAAACAACAACACAACTTCTTCCGACATGATTTCTCAAGGAACCTTTGAGGCCAAAAAGGTAGCAACACGTCCTAAACTCTCTCTTCCTCAAGACCATATAATATCTGAAAGAAAGCGGCGTGAGAAGCTTAGCCAGCGCTTCATTGCTCTATCTTCCCTTGTTCCCGGACTACAAAAGGTATAAAAAATTCGACTATCACGAATTTTTTACATTTTATTTTGAACCTGACTCAACTCTACAAATTTGACAAATTTGTAAGGTGAAGATTGCATAGGCTTTATGATCTCTGCCTAACGCGGGACTTAAGTTTTTTCTCGATATCTATGTTGAACACATGTTGGATCATTTAGTGTTTACTATTAGTAGGACACACGGTGAATATATAAGAATGTGTTTGTGGTACTATCTTTAACAATTTTTGTCGTCTCGTTTTTCATTGTGCTACTACTAACAGATGGACAAAGTTACTGTTCTTGGAGATGCTATCAAGTACTTGAAGAAATTGCAAGAGAAGGTGAAGGTTCTTGAGGAGGAACAGAACATGAAGAAAAACGTGGAATTTGTGGTGGTTGTGAAGAAATATCACTTATCCAATGATGTTGAAAATTCTTCTGCAGAATCTGGTGGTCCCTTCGACGAGGAACTGCCAGAAATTGAAGCAAGATTTTGTGATAGAAATGTCCTCATAAGAGTTCACTGTGAGAAAATCAAAGGAGTTGTGGAAAAAACAATCCATGAAATTGAGAAACTCAATCTAAAAGTCACCAATAGCAGTTTCATGACATTTGGGAGTTGTGCACTTGATATAACAATTATTGCACAGGTAATCTTTGTTTCAACAAAAGTATTGAATTAAGTTTCAGTAGTTATATTTTAAGACTTTTGACTACTTTAATGAAAAAACTTGCTTATTTACTGTATATCTATCTATGCATAATTTTCTATTATTTTGTTCCAACGAAATTACTACAATTGTCTATCAATATGGAAACTATTTGTGCAATTTGCAGATGGATATGGAATTTTGCATGACGGTGAAGGATCTTGTGAGAAACCTACGCTCAGTTTTTACGTCTTTCATCTAA
Protein sequence

>MS.gene053631.t1

MENISFIRGFPEQEMMEDPSFLHHQWHLSSINESNSLPIGTAFGDTSQHHSYVYPNFNPRTSMETAQTLETQFVPYPNLLSFVDLNQLNQLGLVKPKDEMIGSQNNNTTSSDMISQGTFEAKKVATRPKLSLPQDHIISERKRREKLSQRFIALSSLVPGLQKMDKVTVLGDAIKYLKKLQEKVKVLEEEQNMKKNVEFVVVVKKYHLSNDVENSSAESGGPFDEELPEIEARFCDRNVLIRVHCEKIKGVVEKTIHEIEKLNLKVTNSSFMTFGSCALDITIIAQMDMEFCMTVKDLVRNLRSVFTSFI