Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053748.t1 | XP_013453992.2 | 98.7 | 774 | 10 | 0 | 1 | 774 | 1 | 774 | 0.00E+00 | 1419.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053748.t1 | Q8L751 | 70.3 | 814 | 202 | 5 | 1 | 774 | 1 | 814 | 2.1e-300 | 1032.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053748.t1 | A0A396HTZ2 | 98.7 | 774 | 10 | 0 | 1 | 774 | 1 | 774 | 0.0e+00 | 1419.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene94096 | MS.gene053748 | PPI |
| MS.gene73924 | MS.gene053748 | PPI |
| MS.gene045181 | MS.gene053748 | PPI |
| MS.gene031848 | MS.gene053748 | PPI |
| MS.gene30772 | MS.gene053748 | PPI |
| MS.gene35664 | MS.gene053748 | PPI |
| MS.gene067071 | MS.gene053748 | PPI |
| MS.gene29058 | MS.gene053748 | PPI |
| MS.gene053748 | MS.gene75538 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053748.t1 | MTR_5g459350 | 98.541 | 754 | 11 | 0 | 21 | 774 | 1 | 754 | 0.0 | 1551 |
| MS.gene053748.t1 | MTR_3g073040 | 77.204 | 794 | 153 | 7 | 1 | 777 | 1 | 783 | 0.0 | 1248 |
| MS.gene053748.t1 | MTR_3g073040 | 77.078 | 794 | 153 | 8 | 1 | 777 | 1 | 782 | 0.0 | 1242 |
| MS.gene053748.t1 | MTR_3g097270 | 61.235 | 761 | 267 | 8 | 11 | 769 | 15 | 749 | 0.0 | 953 |
| MS.gene053748.t1 | MTR_3g073040 | 76.490 | 621 | 118 | 7 | 1 | 604 | 1 | 610 | 0.0 | 945 |
| MS.gene053748.t1 | MTR_3g086340 | 56.473 | 811 | 298 | 15 | 1 | 765 | 1 | 802 | 0.0 | 886 |
| MS.gene053748.t1 | MTR_4g101410 | 54.315 | 788 | 317 | 12 | 1 | 773 | 6 | 765 | 0.0 | 830 |
| MS.gene053748.t1 | MTR_3g086340 | 54.824 | 653 | 245 | 11 | 1 | 609 | 1 | 647 | 0.0 | 688 |
| MS.gene053748.t1 | MTR_4g101410 | 61.338 | 538 | 198 | 5 | 242 | 773 | 1 | 534 | 0.0 | 673 |
| MS.gene053748.t1 | MTR_7g061830 | 34.565 | 379 | 220 | 13 | 397 | 759 | 73 | 439 | 2.84e-48 | 177 |
| MS.gene053748.t1 | MTR_4g064110 | 32.517 | 449 | 242 | 21 | 334 | 751 | 9 | 427 | 9.87e-46 | 170 |
| MS.gene053748.t1 | MTR_4g115490 | 44.199 | 181 | 26 | 4 | 304 | 482 | 43 | 150 | 8.91e-28 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053748.t1 | AT4G08180 | 69.353 | 819 | 201 | 8 | 1 | 774 | 1 | 814 | 0.0 | 1122 |
| MS.gene053748.t1 | AT4G08180 | 69.353 | 819 | 201 | 8 | 1 | 774 | 1 | 814 | 0.0 | 1122 |
| MS.gene053748.t1 | AT4G08180 | 69.231 | 819 | 201 | 9 | 1 | 774 | 1 | 813 | 0.0 | 1115 |
| MS.gene053748.t1 | AT4G08180 | 68.864 | 819 | 204 | 9 | 1 | 774 | 1 | 813 | 0.0 | 1110 |
| MS.gene053748.t1 | AT2G31020 | 68.256 | 797 | 198 | 12 | 1 | 766 | 1 | 773 | 0.0 | 1045 |
| MS.gene053748.t1 | AT1G13170 | 58.333 | 804 | 288 | 13 | 1 | 763 | 1 | 798 | 0.0 | 917 |
| MS.gene053748.t1 | AT1G13170 | 56.848 | 825 | 288 | 14 | 1 | 763 | 1 | 819 | 0.0 | 904 |
| MS.gene053748.t1 | AT4G22540 | 51.943 | 772 | 307 | 10 | 1 | 769 | 6 | 716 | 0.0 | 806 |
| MS.gene053748.t1 | AT4G22540 | 51.943 | 772 | 307 | 10 | 1 | 769 | 6 | 716 | 0.0 | 806 |
| MS.gene053748.t1 | AT4G12460 | 53.939 | 749 | 270 | 13 | 32 | 769 | 4 | 688 | 0.0 | 805 |
| MS.gene053748.t1 | AT4G12460 | 53.939 | 749 | 270 | 13 | 32 | 769 | 4 | 688 | 0.0 | 805 |
| MS.gene053748.t1 | AT4G12460 | 53.867 | 750 | 270 | 14 | 32 | 769 | 4 | 689 | 0.0 | 800 |
| MS.gene053748.t1 | AT2G31030 | 77.037 | 405 | 86 | 1 | 362 | 766 | 91 | 488 | 0.0 | 680 |
| MS.gene053748.t1 | AT4G12460 | 52.121 | 660 | 241 | 13 | 32 | 680 | 4 | 599 | 0.0 | 675 |
| MS.gene053748.t1 | AT4G12460 | 52.042 | 661 | 241 | 14 | 32 | 680 | 4 | 600 | 0.0 | 671 |
| MS.gene053748.t1 | AT4G12460 | 58.704 | 540 | 183 | 5 | 230 | 769 | 1 | 500 | 0.0 | 657 |
| MS.gene053748.t1 | AT4G12460 | 58.704 | 540 | 183 | 5 | 230 | 769 | 1 | 500 | 0.0 | 657 |
| MS.gene053748.t1 | AT4G12460 | 58.704 | 540 | 183 | 5 | 230 | 769 | 1 | 500 | 0.0 | 657 |
| MS.gene053748.t1 | AT4G22540 | 57.963 | 540 | 192 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene053748.t1 | AT4G22540 | 57.963 | 540 | 192 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene053748.t1 | AT4G22540 | 57.963 | 540 | 192 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene053748.t1 | AT4G22540 | 57.963 | 540 | 192 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene053748.t1 | AT4G12460 | 70.190 | 369 | 101 | 2 | 401 | 769 | 1 | 360 | 0.0 | 564 |
| MS.gene053748.t1 | AT1G77730 | 48.462 | 260 | 118 | 1 | 36 | 279 | 2 | 261 | 9.43e-77 | 249 |
| MS.gene053748.t1 | AT3G09300 | 36.658 | 371 | 203 | 16 | 399 | 751 | 75 | 431 | 4.92e-51 | 185 |
| MS.gene053748.t1 | AT5G02100 | 34.884 | 387 | 218 | 14 | 355 | 732 | 11 | 372 | 9.62e-49 | 178 |
| MS.gene053748.t1 | AT5G02100 | 34.884 | 387 | 218 | 14 | 355 | 732 | 38 | 399 | 1.46e-48 | 178 |
| MS.gene053748.t1 | AT5G59420 | 34.946 | 372 | 212 | 14 | 397 | 751 | 68 | 426 | 7.88e-46 | 171 |
| MS.gene053748.t1 | AT5G02100 | 33.798 | 287 | 168 | 9 | 355 | 637 | 38 | 306 | 2.04e-34 | 134 |
Find 220 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAATATACAATATCATTT+TGG | 0.118580 | 5.1:+45987769 | MS.gene053748:CDS |
| TTGAAACCTTTCTCAGTTTC+AGG | 0.130758 | 5.1:-45983713 | None:intergenic |
| GCAACTTAAAGAGCAAATTT+TGG | 0.166489 | 5.1:+45987569 | MS.gene053748:CDS |
| GTTGGCCATTCCATTGTCTT+TGG | 0.203387 | 5.1:-45985994 | None:intergenic |
| GAGAAAGGTTTCAAGGTTAT+TGG | 0.213209 | 5.1:+45983722 | MS.gene053748:CDS |
| AGGGTACAGGTTGGAAATTT+TGG | 0.226618 | 5.1:+45987539 | MS.gene053748:CDS |
| TTCCATAGCATTTGCGCTTC+TGG | 0.238422 | 5.1:-45988149 | None:intergenic |
| CAGTCTTTGGTCATTGATTA+AGG | 0.238789 | 5.1:+45986493 | MS.gene053748:CDS |
| GATCAGAGGTATTTGGAAAA+TGG | 0.239645 | 5.1:+45989379 | MS.gene053748:CDS |
| GAAAACATTGGCTACATTAA+TGG | 0.241182 | 5.1:+45988055 | MS.gene053748:CDS |
| ATTCTCATCACTCTCAAAAT+TGG | 0.248288 | 5.1:+45983780 | MS.gene053748:CDS |
| TCAGTTTACATGAGTATTCT+TGG | 0.255621 | 5.1:-45987838 | None:intergenic |
| TGTCTTTGTCCCGGTGAAAA+TGG | 0.258915 | 5.1:-45984626 | None:intergenic |
| TGAACTTACCCCGGGATTAA+AGG | 0.268592 | 5.1:+45988235 | MS.gene053748:CDS |
| CTTCAGAAATGGGTGAATTA+TGG | 0.278794 | 5.1:+45983602 | MS.gene053748:CDS |
| GACAGCATGTATTATGTTAA+TGG | 0.279631 | 5.1:+45988089 | MS.gene053748:CDS |
| AAGACCAGATCAGAGGTATT+TGG | 0.284475 | 5.1:+45989372 | MS.gene053748:CDS |
| CTTTCTTATTACAAGATTCA+TGG | 0.296312 | 5.1:+45983668 | MS.gene053748:CDS |
| TATCATCATCCAGTTCTGTT+AGG | 0.301667 | 5.1:+45986321 | MS.gene053748:CDS |
| TTATGGTACAATGCGCATTC+AGG | 0.302819 | 5.1:+45987811 | MS.gene053748:CDS |
| AGAGGTTCATTAAAATAAAC+AGG | 0.322549 | 5.1:-45986551 | None:intergenic |
| AAAGCTAAGGCATCGGCTTA+TGG | 0.329463 | 5.1:+45984783 | MS.gene053748:CDS |
| ATGAAACGAAAACCTTTATC+TGG | 0.331208 | 5.1:-45986875 | None:intergenic |
| AATCGACAATTTACGCCATT+TGG | 0.332700 | 5.1:+45984915 | MS.gene053748:CDS |
| TCATTGATTAAGGATAATAT+TGG | 0.334852 | 5.1:+45986503 | MS.gene053748:CDS |
| AAGCTGTATTTGGATCATTA+TGG | 0.351321 | 5.1:+45987794 | MS.gene053748:CDS |
| CAGTGACATGCAACTATCAT+AGG | 0.356777 | 5.1:-45987514 | None:intergenic |
| TGTTAGTGAAGAAGCTATTC+AGG | 0.357117 | 5.1:+45984813 | MS.gene053748:CDS |
| GGATTGATTACAATCTTATC+AGG | 0.360001 | 5.1:-45983692 | None:intergenic |
| ACCGCTACGTAGGAGGATAT+TGG | 0.362491 | 5.1:+45990985 | MS.gene053748:CDS |
| AAAACATTGGCTACATTAAT+GGG | 0.370877 | 5.1:+45988056 | MS.gene053748:CDS |
| AAGAGAAAGGTGTCAGTCTT+TGG | 0.374372 | 5.1:+45986480 | MS.gene053748:CDS |
| TATCTGGAATACTTCAGAAA+TGG | 0.374717 | 5.1:+45983591 | MS.gene053748:CDS |
| TAAACCGTTTAATCCATTAC+TGG | 0.378853 | 5.1:+45986829 | MS.gene053748:CDS |
| ATACTATTATGAATCTCATT+CGG | 0.380210 | 5.1:-45991172 | None:intergenic |
| TCAAAGATGCTGTGAAGAAA+TGG | 0.381841 | 5.1:+45986583 | MS.gene053748:CDS |
| CAAAATTGGCGTAAGCCTTT+CGG | 0.382485 | 5.1:+45983794 | MS.gene053748:CDS |
| GGGAACCACGATGGTTTGCC+AGG | 0.382501 | 5.1:+45990943 | MS.gene053748:CDS |
| ATCAGAGGTATTTGGAAAAT+GGG | 0.383734 | 5.1:+45989380 | MS.gene053748:CDS |
| CTCGTTGGGAATGGAATATC+TGG | 0.384420 | 5.1:+45983575 | MS.gene053748:CDS |
| TGTCTGTCTTTGCAGGTTCA+AGG | 0.396316 | 5.1:+45988011 | MS.gene053748:intron |
| TACTATTATGAATCTCATTC+GGG | 0.397116 | 5.1:-45991171 | None:intergenic |
| GATCCTTCTATTAGATCTGT+TGG | 0.397837 | 5.1:+45986404 | MS.gene053748:CDS |
| GGTACTGTTCAGGATAGAAA+TGG | 0.403022 | 5.1:+45988032 | MS.gene053748:CDS |
| TTCATACGGCGGTTGACGTT+AGG | 0.413163 | 5.1:+45983543 | MS.gene053748:CDS |
| GATTGGTACGGAGGAAGATT+CGG | 0.417845 | 5.1:-45983471 | None:intergenic |
| CCGCTACGTAGGAGGATATT+GGG | 0.418285 | 5.1:+45990986 | MS.gene053748:CDS |
| GAGGCTGAATATCCAGATAA+AGG | 0.418986 | 5.1:+45986863 | MS.gene053748:CDS |
| TTAGGATCAACGACCTCGTT+GGG | 0.419990 | 5.1:+45983561 | MS.gene053748:CDS |
| AGCTGCCACCTACAGATTCC+AGG | 0.420280 | 5.1:+45989347 | MS.gene053748:CDS |
| GCAGGTTCAAGGTACTGTTC+AGG | 0.421855 | 5.1:+45988022 | MS.gene053748:intron |
| GGAGGAAGGAAGAGGAGGCA+TGG | 0.425480 | 5.1:-45983435 | None:intergenic |
| ATCATCATCCAGTTCTGTTA+GGG | 0.428400 | 5.1:+45986322 | MS.gene053748:CDS |
| CATTGATTAAGGATAATATT+GGG | 0.435505 | 5.1:+45986504 | MS.gene053748:CDS |
| TCGCCCAGTAATGGATTAAA+CGG | 0.435757 | 5.1:-45986833 | None:intergenic |
| CCCTTCATCATCTGAAGAAA+AGG | 0.437910 | 5.1:-45986351 | None:intergenic |
| CAACATTGAGAATCCTCATA+AGG | 0.438158 | 5.1:-45986760 | None:intergenic |
| ATCTGGAATACTTCAGAAAT+GGG | 0.440833 | 5.1:+45983592 | MS.gene053748:CDS |
| AATGGAGACTATGGTGGAAA+GGG | 0.442329 | 5.1:+45988107 | MS.gene053748:CDS |
| TATTATGTTAATGGAGACTA+TGG | 0.443377 | 5.1:+45988098 | MS.gene053748:CDS |
| TTAAATATACCTTGCTCCAC+TGG | 0.443931 | 5.1:-45987657 | None:intergenic |
| CATCCTCCGTTAGCGGAGAT+TGG | 0.447673 | 5.1:-45983488 | None:intergenic |
| GGATGAAGCCCTAACAGAAC+TGG | 0.451204 | 5.1:-45986330 | None:intergenic |
| ATTCCAACTTCCTTGCTGTC+GGG | 0.451772 | 5.1:-45991012 | None:intergenic |
| TGAAACTGAGAAAGGTTTCA+AGG | 0.454468 | 5.1:+45983715 | MS.gene053748:CDS |
| GGAGGCATGGAGAGGTGAGC+GGG | 0.455669 | 5.1:-45983422 | None:intergenic |
| TCCTTGACCGAGCTTATGAA+TGG | 0.464437 | 5.1:+45986618 | MS.gene053748:CDS |
| GATGAGGTGGAGGAAGGAAG+AGG | 0.468545 | 5.1:-45983443 | None:intergenic |
| TCTAATGTCAAAATACCAAC+AGG | 0.470049 | 5.1:-45987613 | None:intergenic |
| GTCCCAACAGATCTAATAGA+AGG | 0.474628 | 5.1:-45986407 | None:intergenic |
| TCATTAAATGTACCTCCAAA+TGG | 0.475571 | 5.1:-45984930 | None:intergenic |
| TCAGCTCGCGGTGGTTCATA+CGG | 0.475668 | 5.1:+45983529 | MS.gene053748:CDS |
| AAGACAATGGAATGGCCAAC+AGG | 0.476443 | 5.1:+45985997 | MS.gene053748:CDS |
| ATCCTTCTATTAGATCTGTT+GGG | 0.481357 | 5.1:+45986405 | MS.gene053748:CDS |
| GTTATATCTAGTAGGAAACT+TGG | 0.484791 | 5.1:-45988186 | None:intergenic |
| TATGGTACAATGCGCATTCA+GGG | 0.485321 | 5.1:+45987812 | MS.gene053748:CDS |
| CCTCTGATCTGGTCTTAACC+TGG | 0.487702 | 5.1:-45989365 | None:intergenic |
| CGACAATTTACGCCATTTGG+AGG | 0.488709 | 5.1:+45984918 | MS.gene053748:CDS |
| TTCAGGGCAATACCCTTATG+AGG | 0.490577 | 5.1:+45986747 | MS.gene053748:intron |
| CCTTGACCGAGCTTATGAAT+GGG | 0.490785 | 5.1:+45986619 | MS.gene053748:CDS |
| CGAGTTAAGTTATATCTAGT+AGG | 0.495813 | 5.1:-45988194 | None:intergenic |
| GGAAGATGCAAGAGAGTGGT+TGG | 0.497056 | 5.1:+45990922 | MS.gene053748:CDS |
| ATCTCTGTAGTTGGATCCGA+GGG | 0.497057 | 5.1:+45986374 | MS.gene053748:CDS |
| AACATTGAGAATCCTCATAA+GGG | 0.497664 | 5.1:-45986759 | None:intergenic |
| GATGAAGGGATCTCTGTAGT+TGG | 0.498961 | 5.1:+45986365 | MS.gene053748:CDS |
| TGCCAGAAGCGCAAATGCTA+TGG | 0.501284 | 5.1:+45988147 | MS.gene053748:CDS |
| AAGTACAGACCTTTAATCCC+GGG | 0.503491 | 5.1:-45988244 | None:intergenic |
| AATTCCAACTTCCTTGCTGT+CGG | 0.506969 | 5.1:-45991013 | None:intergenic |
| TCATATGTCTCGCCCAGTAA+TGG | 0.509403 | 5.1:-45986842 | None:intergenic |
| GGCTTCTTCCATGATGAGGC+AGG | 0.510248 | 5.1:+45986018 | MS.gene053748:CDS |
| AAATGGGTGAATTATGGTAA+AGG | 0.510547 | 5.1:+45983608 | MS.gene053748:CDS |
| TCTGCTTAAGTAGCAAAAGC+TGG | 0.512576 | 5.1:-45984879 | None:intergenic |
| GGAAAATGGGGAGTATGAGA+TGG | 0.513993 | 5.1:+45989393 | MS.gene053748:CDS |
| TGAGAGAAATGATGCAGTTG+AGG | 0.516891 | 5.1:+45986250 | MS.gene053748:CDS |
| GACTATGGTGGAAAGGGGAA+AGG | 0.516943 | 5.1:+45988113 | MS.gene053748:CDS |
| TCTGCCTATGCTTCAACTGA+TGG | 0.517519 | 5.1:+45986797 | MS.gene053748:CDS |
| AGAGCAGATCCGATGATAAA+CGG | 0.522425 | 5.1:+45984599 | MS.gene053748:CDS |
| ATCAACGACCTCGTTGGGAA+TGG | 0.522674 | 5.1:+45983566 | MS.gene053748:CDS |
| TTGACATTAGAATTTGATGA+TGG | 0.523339 | 5.1:+45987625 | MS.gene053748:CDS |
| CTCATGTAAACTGAAATTCA+AGG | 0.524169 | 5.1:+45987847 | MS.gene053748:CDS |
| TTCGGAGCGAGATGAGGTGG+AGG | 0.524235 | 5.1:-45983453 | None:intergenic |
| GGTAAGTTCATTTAATGTGA+TGG | 0.526479 | 5.1:-45988222 | None:intergenic |
| ATTACTGGGCGAGACATATG+AGG | 0.531058 | 5.1:+45986844 | MS.gene053748:CDS |
| CCCATTCATAAGCTCGGTCA+AGG | 0.532735 | 5.1:-45986619 | None:intergenic |
| ATCAATCCTGAAACTGAGAA+AGG | 0.532894 | 5.1:+45983707 | MS.gene053748:CDS |
| GTACTGTTCAGGATAGAAAT+GGG | 0.533845 | 5.1:+45988033 | MS.gene053748:CDS |
| ATGAGGCAGGAAAAGTTTAG+TGG | 0.534064 | 5.1:+45986031 | MS.gene053748:CDS |
| GATGTCCATCAGTGAGCTGA+TGG | 0.534460 | 5.1:+45984729 | MS.gene053748:CDS |
| ACATTAAATGAACTTACCCC+GGG | 0.536443 | 5.1:+45988227 | MS.gene053748:CDS |
| AATCCAGAGGTTAGTGCAAG+TGG | 0.537993 | 5.1:+45986212 | MS.gene053748:intron |
| GAAGATGCAAGAGAGTGGTT+GGG | 0.539315 | 5.1:+45990923 | MS.gene053748:CDS |
| GGGTGAATTATGGTAAAGGA+TGG | 0.539666 | 5.1:+45983612 | MS.gene053748:CDS |
| GAAAATGGGGAGTATGAGAT+GGG | 0.539759 | 5.1:+45989394 | MS.gene053748:CDS |
| TTCACAGCATCTTTGAAGAG+AGG | 0.539933 | 5.1:-45986576 | None:intergenic |
| AAAGTACAGACCTTTAATCC+CGG | 0.540120 | 5.1:-45988245 | None:intergenic |
| GAGGTGGAGGAAGGAAGAGG+AGG | 0.544566 | 5.1:-45983440 | None:intergenic |
| GTTACGATTGATAATACGCA+TGG | 0.544599 | 5.1:-45983753 | None:intergenic |
| TCAATTCAACTTGATCCTGT+TGG | 0.546151 | 5.1:+45987598 | MS.gene053748:CDS |
| TGGCTACATTAATGGGAAAG+TGG | 0.548273 | 5.1:+45988063 | MS.gene053748:CDS |
| GTTAGGATCAACGACCTCGT+TGG | 0.548507 | 5.1:+45983560 | MS.gene053748:CDS |
| CACATTAAATGAACTTACCC+CGG | 0.549152 | 5.1:+45988226 | MS.gene053748:CDS |
| AGATTCGGAGCGAGATGAGG+TGG | 0.549375 | 5.1:-45983456 | None:intergenic |
| TCTCCCATCAGTTGAAGCAT+AGG | 0.549881 | 5.1:-45986801 | None:intergenic |
| AAGCCCGACAGCAAGGAAGT+TGG | 0.550357 | 5.1:+45991009 | MS.gene053748:CDS |
| GCTACTTAAGCAGAAGCAGT+TGG | 0.551688 | 5.1:+45984888 | MS.gene053748:CDS |
| TTTCGGTGAAATTCACCTCA+AGG | 0.552799 | 5.1:+45983811 | MS.gene053748:CDS |
| AAACCGTTTAATCCATTACT+GGG | 0.553076 | 5.1:+45986830 | MS.gene053748:CDS |
| CAAGTGCAGTGTCTTTGTCC+CGG | 0.553859 | 5.1:-45984635 | None:intergenic |
| GATGGCTCCAGTGGACAATA+CGG | 0.554147 | 5.1:+45984747 | MS.gene053748:CDS |
| GATTGATCGCAATCCTCACC+AGG | 0.554267 | 5.1:+45987880 | MS.gene053748:CDS |
| TCGTCTAAAAGATGCTCAGA+AGG | 0.554380 | 5.1:-45991062 | None:intergenic |
| AAGCGCAAATGCTATGGAAG+CGG | 0.554969 | 5.1:+45988153 | MS.gene053748:CDS |
| GCATGTCACTGTGAGGGTAC+AGG | 0.555198 | 5.1:+45987526 | MS.gene053748:CDS |
| CTGCCTATGCTTCAACTGAT+GGG | 0.557550 | 5.1:+45986798 | MS.gene053748:CDS |
| GAGCGAGATGAGGTGGAGGA+AGG | 0.563441 | 5.1:-45983449 | None:intergenic |
| GGATGATGATATAAAATCAC+GGG | 0.563992 | 5.1:-45986309 | None:intergenic |
| AAGGAAGAGGAGGCATGGAG+AGG | 0.564352 | 5.1:-45983430 | None:intergenic |
| AGGATAGTGAGCAAATTATG+AGG | 0.566818 | 5.1:+45984833 | MS.gene053748:CDS |
| TGGAGCCATCAGCTCACTGA+TGG | 0.567673 | 5.1:-45984734 | None:intergenic |
| TAATGGAGACTATGGTGGAA+AGG | 0.568198 | 5.1:+45988106 | MS.gene053748:CDS |
| GGTACCTACCGCTACGTAGG+AGG | 0.568840 | 5.1:+45990978 | MS.gene053748:CDS |
| TCGTGATGATCGAGTGGCAT+GGG | 0.570755 | 5.1:+45984669 | MS.gene053748:CDS |
| ATGGAGACTATGGTGGAAAG+GGG | 0.573178 | 5.1:+45988108 | MS.gene053748:CDS |
| CAGGATCAAGTTGAATTGAT+CGG | 0.574504 | 5.1:-45987594 | None:intergenic |
| GCAATCACATCCTCCGTTAG+CGG | 0.575281 | 5.1:-45983495 | None:intergenic |
| AATGGAAAACCGTTTATCAT+CGG | 0.575297 | 5.1:-45984608 | None:intergenic |
| CAGTGAGCTGATGGCTCCAG+TGG | 0.576173 | 5.1:+45984738 | MS.gene053748:CDS |
| CGGTCAAAGATATGTTTCCT+CGG | 0.576214 | 5.1:+45984707 | MS.gene053748:CDS |
| TTATATCTAGTAGGAAACTT+GGG | 0.576474 | 5.1:-45988185 | None:intergenic |
| AGGCATCGGCTTATGGAAGA+AGG | 0.576568 | 5.1:+45984790 | MS.gene053748:CDS |
| TCCGTACCAATCTCCGCTAA+CGG | 0.578210 | 5.1:+45983482 | MS.gene053748:CDS |
| GTAAAGGATGGAGGCATCGA+TGG | 0.579108 | 5.1:+45983624 | MS.gene053748:CDS |
| TATGTTAATGGAGACTATGG+TGG | 0.579388 | 5.1:+45988101 | MS.gene053748:CDS |
| GAAACGGCCGTATTGTCCAC+TGG | 0.581171 | 5.1:-45984754 | None:intergenic |
| CCGTTTCAACTGAAAAGCTA+AGG | 0.584451 | 5.1:+45984770 | MS.gene053748:CDS |
| GCTAGAGCAGCGACAACGGC+AGG | 0.585865 | 5.1:+45989435 | MS.gene053748:CDS |
| ACTGCGGAGATGCAGCAGAA+AGG | 0.588268 | 5.1:-45983383 | None:intergenic |
| CAACTGAAAAGCTAAGGCAT+CGG | 0.590488 | 5.1:+45984776 | MS.gene053748:CDS |
| TTGAGGTGAATTTCACCGAA+AGG | 0.590833 | 5.1:-45983809 | None:intergenic |
| TGGATGATGATATAAAATCA+CGG | 0.592514 | 5.1:-45986310 | None:intergenic |
| AGGAGGCATGGAGAGGTGAG+CGG | 0.594704 | 5.1:-45983423 | None:intergenic |
| CGATGGTTCGTGTTGCAAGA+TGG | 0.594774 | 5.1:+45983641 | MS.gene053748:CDS |
| GGTTAGTGCAAGTGGGTCTG+AGG | 0.595559 | 5.1:+45986220 | MS.gene053748:CDS |
| GGATAGAAATGGGAAAACAT+TGG | 0.596727 | 5.1:+45988043 | MS.gene053748:CDS |
| GTAGACAGAGAAGGTTGACC+TGG | 0.597103 | 5.1:+45985949 | MS.gene053748:intron |
| TTGATCATTGTAGACAGAGA+AGG | 0.597497 | 5.1:+45985940 | MS.gene053748:intron |
| TGAATTATGGTAAAGGATGG+AGG | 0.598506 | 5.1:+45983615 | MS.gene053748:CDS |
| TACGGCTAGAGCAGCGACAA+CGG | 0.603059 | 5.1:+45989431 | MS.gene053748:CDS |
| ACATATAAAAGCACCTGGTG+AGG | 0.604072 | 5.1:-45987893 | None:intergenic |
| CTCGTGATGATCGAGTGGCA+TGG | 0.604319 | 5.1:+45984668 | MS.gene053748:CDS |
| GGAGGATGTGATTGCAGCCG+CGG | 0.604714 | 5.1:+45983503 | MS.gene053748:CDS |
| GGAAGATTCGGAGCGAGATG+AGG | 0.607636 | 5.1:-45983459 | None:intergenic |
| TTGATGAGAGCCAAAGACAA+TGG | 0.608013 | 5.1:+45985984 | MS.gene053748:CDS |
| GATTAAGGATAATATTGGGA+AGG | 0.610369 | 5.1:+45986508 | MS.gene053748:CDS |
| TCATCATGGAAGAAGCCTGT+TGG | 0.611229 | 5.1:-45986012 | None:intergenic |
| AGTGACATGCAACTATCATA+GGG | 0.611714 | 5.1:-45987513 | None:intergenic |
| AACAGGCTTCTTCCATGATG+AGG | 0.612253 | 5.1:+45986014 | MS.gene053748:CDS |
| TCCGTTAGCGGAGATTGGTA+CGG | 0.613315 | 5.1:-45983483 | None:intergenic |
| TGAAATATTCCAGTGGAGCA+AGG | 0.613705 | 5.1:+45987648 | MS.gene053748:CDS |
| CATCTTTGAAGAGAGGAGAG+AGG | 0.617333 | 5.1:-45986569 | None:intergenic |
| GGTCTTAACCTGGAATCTGT+AGG | 0.619295 | 5.1:-45989355 | None:intergenic |
| CCACCGCGAGCTGACTTCCG+CGG | 0.622520 | 5.1:-45983520 | None:intergenic |
| CAGCCGCGGAAGTCAGCTCG+CGG | 0.624216 | 5.1:+45983517 | MS.gene053748:CDS |
| GTCACTGTGAGGGTACAGGT+TGG | 0.625184 | 5.1:+45987530 | MS.gene053748:CDS |
| CCGCGGAAGTCAGCTCGCGG+TGG | 0.625889 | 5.1:+45983520 | MS.gene053748:CDS |
| CAGATATTCCATTCCCAACG+AGG | 0.628322 | 5.1:-45983574 | None:intergenic |
| TATTGGGAAGCCCGACAGCA+AGG | 0.630226 | 5.1:+45991002 | MS.gene053748:CDS |
| GAGAGCCAAAGACAATGGAA+TGG | 0.630637 | 5.1:+45985989 | MS.gene053748:CDS |
| GTGAGCGGGAGATTGATGAT+CGG | 0.634010 | 5.1:-45983408 | None:intergenic |
| GGAACCACGATGGTTTGCCA+GGG | 0.636801 | 5.1:+45990944 | MS.gene053748:CDS |
| TCAGAGGTATTTGGAAAATG+GGG | 0.638243 | 5.1:+45989381 | MS.gene053748:CDS |
| ATCCAGAGGTTAGTGCAAGT+GGG | 0.638560 | 5.1:+45986213 | MS.gene053748:intron |
| TGATGATCGAGTGGCATGGG+TGG | 0.639610 | 5.1:+45984672 | MS.gene053748:CDS |
| TGAGACTCGTGATGATCGAG+TGG | 0.643755 | 5.1:+45984663 | MS.gene053748:CDS |
| CTTAACCTGGAATCTGTAGG+TGG | 0.645465 | 5.1:-45989352 | None:intergenic |
| CTTGACCGAGCTTATGAATG+GGG | 0.646861 | 5.1:+45986620 | MS.gene053748:CDS |
| GATCTCTGTAGTTGGATCCG+AGG | 0.648525 | 5.1:+45986373 | MS.gene053748:CDS |
| ATATCCTCCTACGTAGCGGT+AGG | 0.649744 | 5.1:-45990982 | None:intergenic |
| GCTCGGAAGATGCAAGAGAG+TGG | 0.652263 | 5.1:+45990918 | MS.gene053748:CDS |
| CCCAATATCCTCCTACGTAG+CGG | 0.655345 | 5.1:-45990986 | None:intergenic |
| TATCTAGTAGGAAACTTGGG+AGG | 0.655367 | 5.1:-45988182 | None:intergenic |
| GTACCAATCTCCGCTAACGG+AGG | 0.655566 | 5.1:+45983485 | MS.gene053748:CDS |
| CTCTGTAGTTGGATCCGAGG+GGG | 0.656234 | 5.1:+45986376 | MS.gene053748:CDS |
| CCAGGTTAAGACCAGATCAG+AGG | 0.659521 | 5.1:+45989365 | MS.gene053748:CDS |
| AGAGTGGTTGGGAACCACGA+TGG | 0.662475 | 5.1:+45990934 | MS.gene053748:CDS |
| AGTACAGACCTTTAATCCCG+GGG | 0.676889 | 5.1:-45988243 | None:intergenic |
| GTTAGCGGAGATTGGTACGG+AGG | 0.678256 | 5.1:-45983480 | None:intergenic |
| AGCTCACTGATGGACATCCG+AGG | 0.683633 | 5.1:-45984724 | None:intergenic |
| AGATTGATGATCGGAGACTG+CGG | 0.689607 | 5.1:-45983399 | None:intergenic |
| AGCGGTACCTACCGCTACGT+AGG | 0.696997 | 5.1:+45990975 | MS.gene053748:CDS |
| GACATGCAACTATCATAGGG+TGG | 0.699648 | 5.1:-45987510 | None:intergenic |
| TCTCTGTAGTTGGATCCGAG+GGG | 0.707988 | 5.1:+45986375 | MS.gene053748:CDS |
| ATGATGGTGAAATATTCCAG+TGG | 0.713311 | 5.1:+45987641 | MS.gene053748:CDS |
| GCTCGCGGTGGTTCATACGG+CGG | 0.713933 | 5.1:+45983532 | MS.gene053748:CDS |
| GAGGTTCATTAAAATAAACA+GGG | 0.715478 | 5.1:-45986550 | None:intergenic |
| GATAGTTGCATGTCACTGTG+AGG | 0.719382 | 5.1:+45987519 | MS.gene053748:CDS |
| TTATCCCTGGCAAACCATCG+TGG | 0.720845 | 5.1:-45990948 | None:intergenic |
| ATAGTTGCATGTCACTGTGA+GGG | 0.729407 | 5.1:+45987520 | MS.gene053748:CDS |
| ATGGGTGGAAGCTTTGTATG+CGG | 0.739186 | 5.1:+45984687 | MS.gene053748:CDS |
| TTTGCCAGGGATAAAACTAG+CGG | 0.762542 | 5.1:+45990957 | MS.gene053748:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 45983377 | 45991179 | 45983377 | ID=MS.gene053748 |
| chr5.1 | mRNA | 45983377 | 45991179 | 45983377 | ID=MS.gene053748.t1;Parent=MS.gene053748 |
| chr5.1 | exon | 45983377 | 45983832 | 45983377 | ID=MS.gene053748.t1.exon1;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45983377 | 45983832 | 45983377 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45984583 | 45984939 | 45984583 | ID=MS.gene053748.t1.exon2;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45984583 | 45984939 | 45984583 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45985953 | 45986052 | 45985953 | ID=MS.gene053748.t1.exon3;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45985953 | 45986052 | 45985953 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45986219 | 45986649 | 45986219 | ID=MS.gene053748.t1.exon4;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45986219 | 45986649 | 45986219 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45986752 | 45986907 | 45986752 | ID=MS.gene053748.t1.exon5;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45986752 | 45986907 | 45986752 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45987502 | 45987669 | 45987502 | ID=MS.gene053748.t1.exon6;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45987502 | 45987669 | 45987502 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45987761 | 45987901 | 45987761 | ID=MS.gene053748.t1.exon7;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45987761 | 45987901 | 45987761 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45988026 | 45988256 | 45988026 | ID=MS.gene053748.t1.exon8;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45988026 | 45988256 | 45988026 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45989343 | 45989456 | 45989343 | ID=MS.gene053748.t1.exon9;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45989343 | 45989456 | 45989343 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45990918 | 45991086 | 45990918 | ID=MS.gene053748.t1.exon10;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45990918 | 45991086 | 45990918 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| chr5.1 | exon | 45991163 | 45991179 | 45991163 | ID=MS.gene053748.t1.exon11;Parent=MS.gene053748.t1 |
| chr5.1 | CDS | 45991163 | 45991179 | 45991163 | ID=cds.MS.gene053748.t1;Parent=MS.gene053748.t1 |
| Gene Sequence |
| Protein sequence |
