AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73924

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73924.t1 MTR_5g459350 98.155 759 14 0 21 779 1 759 0.0 1561
MS.gene73924.t1 MTR_3g073040 76.604 795 158 7 1 778 1 784 0.0 1243
MS.gene73924.t1 MTR_3g073040 76.478 795 158 8 1 778 1 783 0.0 1237
MS.gene73924.t1 MTR_3g097270 60.578 761 272 8 11 769 15 749 0.0 949
MS.gene73924.t1 MTR_3g073040 76.006 621 121 7 1 604 1 610 0.0 944
MS.gene73924.t1 MTR_3g086340 56.350 811 299 15 1 765 1 802 0.0 890
MS.gene73924.t1 MTR_4g101410 54.177 790 315 13 1 773 6 765 0.0 830
MS.gene73924.t1 MTR_3g086340 54.824 653 245 11 1 609 1 647 0.0 692
MS.gene73924.t1 MTR_4g101410 61.338 538 198 5 242 773 1 534 0.0 675
MS.gene73924.t1 MTR_7g061830 34.565 379 220 13 397 759 73 439 6.16e-49 179
MS.gene73924.t1 MTR_4g064110 32.739 449 241 21 334 751 9 427 2.76e-46 172
MS.gene73924.t1 MTR_4g115490 44.199 181 26 4 304 482 43 150 6.89e-28 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73924.t1 AT4G08180 69.353 819 201 8 1 774 1 814 0.0 1125
MS.gene73924.t1 AT4G08180 69.353 819 201 8 1 774 1 814 0.0 1125
MS.gene73924.t1 AT4G08180 69.231 819 201 9 1 774 1 813 0.0 1118
MS.gene73924.t1 AT4G08180 68.864 819 204 9 1 774 1 813 0.0 1112
MS.gene73924.t1 AT2G31020 68.593 796 197 10 1 766 1 773 0.0 1051
MS.gene73924.t1 AT1G13170 57.836 804 292 12 1 763 1 798 0.0 919
MS.gene73924.t1 AT1G13170 56.364 825 292 13 1 763 1 819 0.0 906
MS.gene73924.t1 AT4G22540 51.813 772 308 9 1 769 6 716 0.0 808
MS.gene73924.t1 AT4G22540 51.813 772 308 9 1 769 6 716 0.0 808
MS.gene73924.t1 AT4G12460 53.805 749 271 13 32 769 4 688 0.0 803
MS.gene73924.t1 AT4G12460 53.805 749 271 13 32 769 4 688 0.0 803
MS.gene73924.t1 AT4G12460 53.733 750 271 14 32 769 4 689 0.0 798
MS.gene73924.t1 AT2G31030 77.284 405 85 1 362 766 91 488 0.0 681
MS.gene73924.t1 AT4G12460 51.970 660 242 13 32 680 4 599 0.0 674
MS.gene73924.t1 AT4G12460 51.891 661 242 14 32 680 4 600 0.0 669
MS.gene73924.t1 AT4G22540 57.963 540 192 4 230 769 1 505 0.0 657
MS.gene73924.t1 AT4G22540 57.963 540 192 4 230 769 1 505 0.0 657
MS.gene73924.t1 AT4G22540 57.963 540 192 4 230 769 1 505 0.0 657
MS.gene73924.t1 AT4G22540 57.963 540 192 4 230 769 1 505 0.0 657
MS.gene73924.t1 AT4G12460 58.704 540 183 5 230 769 1 500 0.0 656
MS.gene73924.t1 AT4G12460 58.704 540 183 5 230 769 1 500 0.0 656
MS.gene73924.t1 AT4G12460 58.704 540 183 5 230 769 1 500 0.0 656
MS.gene73924.t1 AT4G12460 70.190 369 101 2 401 769 1 360 0.0 563
MS.gene73924.t1 AT1G77730 48.462 260 118 1 36 279 2 261 1.25e-76 249
MS.gene73924.t1 AT3G09300 36.658 371 203 16 399 751 75 431 1.66e-51 187
MS.gene73924.t1 AT5G02100 34.884 387 218 14 355 732 11 372 2.22e-49 180
MS.gene73924.t1 AT5G02100 34.884 387 218 14 355 732 38 399 3.31e-49 180
MS.gene73924.t1 AT5G59420 34.677 372 213 14 397 751 68 426 5.13e-46 171
MS.gene73924.t1 AT5G02100 33.445 299 172 10 355 649 38 313 1.59e-34 135

Find 217 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATATACAATATCATTT+TGG 0.118580 5.2:+50422384 MS.gene73924:CDS
TTGAATCCTTTCTCAGTTTC+AGG 0.126035 5.2:-50418309 None:intergenic
GCAACTTAAAGAGCAAATTT+TGG 0.166489 5.2:+50422184 MS.gene73924:CDS
GTTGGCCATTCCATTGTCTT+TGG 0.203387 5.2:-50420608 None:intergenic
GATCAAAGGTATTTGGAAAA+TGG 0.210286 5.2:+50423167 MS.gene73924:CDS
AGGGTACAGGTTGGAAATTT+TGG 0.226618 5.2:+50422154 MS.gene73924:CDS
GAGAAAGGATTCAAGGTTAT+TGG 0.235224 5.2:+50418318 MS.gene73924:CDS
CAGTCTTTGGTCATTGATTA+AGG 0.238789 5.2:+50421108 MS.gene73924:CDS
GAAAACATTGGCTACATTAA+TGG 0.241182 5.2:+50422669 MS.gene73924:CDS
ATTCTCATCACTCTCAAAAT+TGG 0.245623 5.2:+50418376 MS.gene73924:CDS
AAGACCAGATCAAAGGTATT+TGG 0.252435 5.2:+50423160 MS.gene73924:CDS
TCAGTTTACATGAGTATTCT+TGG 0.255621 5.2:-50422453 None:intergenic
TGTCTTTGTCCCGGTGAAAA+TGG 0.258915 5.2:-50419257 None:intergenic
CTTCAGAAATGGGTGAATTA+TGG 0.278794 5.2:+50418198 MS.gene73924:CDS
GACAGCATGTATTATGTTAA+TGG 0.279631 5.2:+50422703 MS.gene73924:CDS
TTCCATAGTATTTGCGCTTC+TGG 0.281518 5.2:-50422763 None:intergenic
TGAACTTACCCCAGGATTAA+AGG 0.295249 5.2:+50422849 MS.gene73924:CDS
CTTTCTTATTACAAGATTCA+TGG 0.296312 5.2:+50418264 MS.gene73924:CDS
TATCATCATCCAGTTCTGTT+AGG 0.301667 5.2:+50420936 MS.gene73924:CDS
TTATGGTACAATGCGCATTC+AGG 0.302819 5.2:+50422426 MS.gene73924:CDS
AGAGGTTCATTAAAATAAAC+AGG 0.322549 5.2:-50421166 None:intergenic
AAAGCTAAGGCATCGGCTTA+TGG 0.329463 5.2:+50419414 MS.gene73924:CDS
ATGAAACGAAAACCTTTATC+TGG 0.331208 5.2:-50421490 None:intergenic
ATCAAAGGTATTTGGAAAAT+GGG 0.332133 5.2:+50423168 MS.gene73924:CDS
TCATTGATTAAGGATAATAT+TGG 0.334852 5.2:+50421118 MS.gene73924:CDS
AATCGACAATTTACGCCATT+TGG 0.342761 5.2:+50419546 MS.gene73924:CDS
CAGTGACATGCAATTATCAT+AGG 0.348790 5.2:-50422129 None:intergenic
AAGCTGTATTTGGATCATTA+TGG 0.351321 5.2:+50422409 MS.gene73924:CDS
TGCCAGAAGCGCAAATACTA+TGG 0.356257 5.2:+50422761 MS.gene73924:CDS
TGTTAGTGAAGAAGCTATTC+AGG 0.357117 5.2:+50419444 MS.gene73924:CDS
GGATTGATTACAATCTTATC+AGG 0.360001 5.2:-50418288 None:intergenic
AGCTGCCACCTACGGATTCC+AGG 0.367017 5.2:+50423135 MS.gene73924:CDS
CGAGTTAAGTTATATTTAGT+AGG 0.367844 5.2:-50422808 None:intergenic
ACCGCTACATAGGAGGATAT+TGG 0.370319 5.2:+50424991 MS.gene73924:CDS
AAAACATTGGCTACATTAAT+GGG 0.370877 5.2:+50422670 MS.gene73924:CDS
AAGAGAAAGGTGTCAGTCTT+TGG 0.374372 5.2:+50421095 MS.gene73924:CDS
TATCTGGAATACTTCAGAAA+TGG 0.374717 5.2:+50418187 MS.gene73924:CDS
CAAAATTGGCGTAAGCCTTT+CGG 0.375159 5.2:+50418390 MS.gene73924:CDS
TAAACCGTTTAATCCATTAC+TGG 0.378853 5.2:+50421444 MS.gene73924:CDS
TCAAAGATGCTGTGAAGAAA+TGG 0.381841 5.2:+50421198 MS.gene73924:CDS
TGTCTGTCTTTGCAGGTTCA+AGG 0.396316 5.2:+50422625 MS.gene73924:intron
GATCCTTCTATTAGATCTGT+TGG 0.397837 5.2:+50421019 MS.gene73924:CDS
GGTACTGTTCAGGATAGAAA+TGG 0.403022 5.2:+50422646 MS.gene73924:CDS
CTCGTCGGGAATGGAATATC+TGG 0.403120 5.2:+50418171 MS.gene73924:CDS
AGTTCCAACTTCCTTGTTGT+CGG 0.412476 5.2:-50425019 None:intergenic
CCGCTACATAGGAGGATATT+GGG 0.412728 5.2:+50424992 MS.gene73924:CDS
GATTGGTACGGAGGAAGATT+CGG 0.417845 5.2:-50418067 None:intergenic
GAGGCTGAATATCCAGATAA+AGG 0.418986 5.2:+50421478 MS.gene73924:CDS
GCAGGTTCAAGGTACTGTTC+AGG 0.421855 5.2:+50422636 MS.gene73924:intron
GGAGGAAGGAAGAGGAGGCA+TGG 0.425480 5.2:-50418031 None:intergenic
CTCATACGGCGGTTGACGTT+AGG 0.426577 5.2:+50418139 MS.gene73924:CDS
ATCATCATCCAGTTCTGTTA+GGG 0.428400 5.2:+50420937 MS.gene73924:CDS
GTTATATTTAGTAGGAAACT+TGG 0.430624 5.2:-50422800 None:intergenic
GTTCCAACTTCCTTGTTGTC+GGG 0.431307 5.2:-50425018 None:intergenic
TTAGGATCAACGACCTCGTC+GGG 0.434385 5.2:+50418157 MS.gene73924:CDS
CATTGATTAAGGATAATATT+GGG 0.435505 5.2:+50421119 MS.gene73924:CDS
TCGCCCAGTAATGGATTAAA+CGG 0.435757 5.2:-50421448 None:intergenic
CCCTTCATCATCTGAAGAAA+AGG 0.437910 5.2:-50420966 None:intergenic
ATCTGGAATACTTCAGAAAT+GGG 0.440833 5.2:+50418188 MS.gene73924:CDS
AATGGAGACTATGGTGGAAA+GGG 0.442329 5.2:+50422721 MS.gene73924:CDS
TATTATGTTAATGGAGACTA+TGG 0.443377 5.2:+50422712 MS.gene73924:CDS
TTAAATGTACCTTGCTCCAC+TGG 0.443931 5.2:-50422272 None:intergenic
TCAGCTCGCGGTGGCTCATA+CGG 0.450923 5.2:+50418125 MS.gene73924:CDS
GGATGAAGCCCTAACAGAAC+TGG 0.451204 5.2:-50420945 None:intergenic
CCTCCTCCGTTAGCGGAGAT+TGG 0.453401 5.2:-50418084 None:intergenic
ATCAACGACCTCGTCGGGAA+TGG 0.455551 5.2:+50418162 MS.gene73924:CDS
AAAGTACAGACCTTTAATCC+TGG 0.460351 5.2:-50422859 None:intergenic
TCCTTGACCGAGCTTATGAA+TGG 0.464437 5.2:+50421233 MS.gene73924:CDS
GATGAGGTGGAGGAAGGAAG+AGG 0.468545 5.2:-50418039 None:intergenic
CCTTTGATCTGGTCTTAACC+TGG 0.468806 5.2:-50423153 None:intergenic
TCTAATGTCAAAATACCAAC+AGG 0.470049 5.2:-50422228 None:intergenic
AAGTACAGACCTTTAATCCT+GGG 0.472066 5.2:-50422858 None:intergenic
GTCCCAACAGATCTAATAGA+AGG 0.474628 5.2:-50421022 None:intergenic
TCATTAAATGTACCTCCAAA+TGG 0.475571 5.2:-50419561 None:intergenic
AAGACAATGGAATGGCCAAC+AGG 0.476443 5.2:+50420611 MS.gene73924:CDS
TGAAACTGAGAAAGGATTCA+AGG 0.478956 5.2:+50418311 MS.gene73924:CDS
GGGAACCACGATGGTTTGCT+AGG 0.480681 5.2:+50424949 MS.gene73924:CDS
ATCCTTCTATTAGATCTGTT+GGG 0.481357 5.2:+50421020 MS.gene73924:CDS
TATGGTACAATGCGCATTCA+GGG 0.485321 5.2:+50422427 MS.gene73924:CDS
CGACAATTTACGCCATTTGG+AGG 0.488709 5.2:+50419549 MS.gene73924:CDS
CCTTGACCGAGCTTATGAAT+GGG 0.490785 5.2:+50421234 MS.gene73924:CDS
TTCAGGGCAATACGCTTATG+AGG 0.492963 5.2:+50421362 MS.gene73924:intron
GGAAGATGCAAGAGAGTGGT+TGG 0.497056 5.2:+50424928 MS.gene73924:CDS
ATCTCTGTAGTTGGATCCGA+GGG 0.497057 5.2:+50420989 MS.gene73924:CDS
GATGAAGGGATCTCTGTAGT+TGG 0.498961 5.2:+50420980 MS.gene73924:CDS
TGAGAGAAATGATGCAGTAG+AGG 0.500636 5.2:+50420865 MS.gene73924:CDS
GATGTCCATCAGCGAGCTGA+TGG 0.501415 5.2:+50419360 MS.gene73924:CDS
GGAGGCATGGAGAGATGAGC+GGG 0.507571 5.2:-50418018 None:intergenic
TCATATGTCTCGCCCAGTAA+TGG 0.509403 5.2:-50421457 None:intergenic
GGCTTCTTCCATGATGAGGC+AGG 0.510248 5.2:+50420632 MS.gene73924:CDS
AAATGGGTGAATTATGGTAA+AGG 0.510547 5.2:+50418204 MS.gene73924:CDS
TTATATTTAGTAGGAAACTT+GGG 0.511351 5.2:-50422799 None:intergenic
TCTGCTTAAGTAGCAAAAGC+TGG 0.512576 5.2:-50419510 None:intergenic
GGAAAATGGGGAGTATGAGA+TGG 0.513993 5.2:+50423181 MS.gene73924:CDS
GACTATGGTGGAAAGGGGAA+AGG 0.516943 5.2:+50422727 MS.gene73924:CDS
TCTGCCTATGCTTCAACTGA+TGG 0.517519 5.2:+50421412 MS.gene73924:CDS
AGAGCAGATCCGATGATAAA+CGG 0.522425 5.2:+50419230 MS.gene73924:CDS
TTGACATTAGAATTTGATGA+TGG 0.523339 5.2:+50422240 MS.gene73924:CDS
CTCATGTAAACTGAAATTCA+AGG 0.524169 5.2:+50422462 MS.gene73924:CDS
TTCGGAGCGAGATGAGGTGG+AGG 0.524235 5.2:-50418049 None:intergenic
ATCAATCCTGAAACTGAGAA+AGG 0.524425 5.2:+50418303 MS.gene73924:CDS
CCAATCTCCGCTAACGGAGG+AGG 0.524579 5.2:+50418084 MS.gene73924:CDS
GGTAAGTTCATTTAATGTGA+TGG 0.529011 5.2:-50422836 None:intergenic
ATTACTGGGCGAGACATATG+AGG 0.531058 5.2:+50421459 MS.gene73924:CDS
CCCATTCATAAGCTCGGTCA+AGG 0.532735 5.2:-50421234 None:intergenic
GTACTGTTCAGGATAGAAAT+GGG 0.533845 5.2:+50422647 MS.gene73924:CDS
ATGAGGCAGGAAAAGTTTAG+TGG 0.534064 5.2:+50420645 MS.gene73924:CDS
AAGCCCGACAACAAGGAAGT+TGG 0.535725 5.2:+50425015 MS.gene73924:CDS
AATCCAGAGGTTAGTGCAAG+TGG 0.537993 5.2:+50420827 MS.gene73924:intron
GAAGATGCAAGAGAGTGGTT+GGG 0.539315 5.2:+50424929 MS.gene73924:CDS
TGATCGAGTGGCATGGGTGG+AGG 0.539487 5.2:+50419306 MS.gene73924:CDS
GGGTGAATTATGGTAAAGGA+TGG 0.539666 5.2:+50418208 MS.gene73924:CDS
GAAAATGGGGAGTATGAGAT+GGG 0.539759 5.2:+50423182 MS.gene73924:CDS
TTCACAGCATCTTTGAAGAG+AGG 0.539933 5.2:-50421191 None:intergenic
GCAATCACCTCCTCCGTTAG+CGG 0.541642 5.2:-50418091 None:intergenic
TCGTCTAAAATATGCTCAGA+AGG 0.543749 5.2:-50425068 None:intergenic
TGGAGCCATCAGCTCGCTGA+TGG 0.543750 5.2:-50419365 None:intergenic
GAGGTGGAGGAAGGAAGAGG+AGG 0.544566 5.2:-50418036 None:intergenic
TCAATTCAACTTGATCCTGT+TGG 0.546151 5.2:+50422213 MS.gene73924:CDS
TGGCTACATTAATGGGAAAG+TGG 0.548273 5.2:+50422677 MS.gene73924:CDS
AGATTCGGAGCGAGATGAGG+TGG 0.549375 5.2:-50418052 None:intergenic
TCTCCCATCAGTTGAAGCAT+AGG 0.549881 5.2:-50421416 None:intergenic
GCTCGCGGTGGCTCATACGG+CGG 0.551876 5.2:+50418128 MS.gene73924:CDS
TTTCGGAGAAATTCACCTCA+AGG 0.552799 5.2:+50418407 MS.gene73924:CDS
AAACCGTTTAATCCATTACT+GGG 0.553076 5.2:+50421445 MS.gene73924:CDS
CAAGTGCAGTGTCTTTGTCC+CGG 0.553859 5.2:-50419266 None:intergenic
GATGGCTCCAGTGGACAATA+CGG 0.554147 5.2:+50419378 MS.gene73924:CDS
ATAGGAAAAGCTGCCACCTA+CGG 0.557288 5.2:+50423127 MS.gene73924:intron
ACATTAAATGAACTTACCCC+AGG 0.557542 5.2:+50422841 MS.gene73924:CDS
CTGCCTATGCTTCAACTGAT+GGG 0.557550 5.2:+50421413 MS.gene73924:CDS
TTGAGGTGAATTTCTCCGAA+AGG 0.558414 5.2:-50418405 None:intergenic
CAGATATTCCATTCCCGACG+AGG 0.562399 5.2:-50418170 None:intergenic
GAGCGAGATGAGGTGGAGGA+AGG 0.563441 5.2:-50418045 None:intergenic
GGATGATGATATAAAATCAC+GGG 0.563992 5.2:-50420924 None:intergenic
AGGATAGCGAGCAAATTATG+AGG 0.565324 5.2:+50419464 MS.gene73924:CDS
TAATGGAGACTATGGTGGAA+AGG 0.568198 5.2:+50422720 MS.gene73924:CDS
GATTGATCGCAATCCTCACC+AGG 0.570708 5.2:+50422495 MS.gene73924:CDS
TCGTGATGATCGAGTGGCAT+GGG 0.570755 5.2:+50419300 MS.gene73924:CDS
AAGCGCAAATACTATGGAAG+CGG 0.571537 5.2:+50422767 MS.gene73924:CDS
ATGGAGACTATGGTGGAAAG+GGG 0.573178 5.2:+50422722 MS.gene73924:CDS
TCCGTTAGCGGAGATTGGTA+CGG 0.574083 5.2:-50418079 None:intergenic
CAGGATCAAGTTGAATTGAT+CGG 0.574504 5.2:-50422209 None:intergenic
GCTACTTAAGCAGAAGCAGT+TGG 0.575074 5.2:+50419519 MS.gene73924:CDS
AATGGAAAACCGTTTATCAT+CGG 0.575297 5.2:-50419239 None:intergenic
GCATGTCACTGTGAGGGTAC+AGG 0.576096 5.2:+50422141 MS.gene73924:CDS
CGGTCAAAGATATGTTTCCT+CGG 0.576214 5.2:+50419338 MS.gene73924:CDS
AGGCATCGGCTTATGGAAGA+AGG 0.576568 5.2:+50419421 MS.gene73924:CDS
TCCGTACCAATCTCCGCTAA+CGG 0.578210 5.2:+50418078 MS.gene73924:CDS
GTAAAGGATGGAGGCATCGA+TGG 0.579108 5.2:+50418220 MS.gene73924:CDS
TATGTTAATGGAGACTATGG+TGG 0.579388 5.2:+50422715 MS.gene73924:CDS
GAAACGGCCGTATTGTCCAC+TGG 0.581171 5.2:-50419385 None:intergenic
CAGCGAGCTGATGGCTCCAG+TGG 0.583165 5.2:+50419369 MS.gene73924:CDS
CCGTTTCAACTGAAAAGCTA+AGG 0.584451 5.2:+50419401 MS.gene73924:CDS
GGAGGAGGTGATTGCAGCCG+CGG 0.584807 5.2:+50418099 MS.gene73924:CDS
GCTAGAGCAGCGACAACGGC+AGG 0.585865 5.2:+50423223 MS.gene73924:CDS
ACTGCGGAGATGCAGCAGAA+AGG 0.588268 5.2:-50417979 None:intergenic
CAACTGAAAAGCTAAGGCAT+CGG 0.590488 5.2:+50419407 MS.gene73924:CDS
GTTAGGATCAACGACCTCGT+CGG 0.592124 5.2:+50418156 MS.gene73924:CDS
TGGATGATGATATAAAATCA+CGG 0.592514 5.2:-50420925 None:intergenic
CGATGGTTCGTGTTGCAAGA+TGG 0.594774 5.2:+50418237 MS.gene73924:CDS
TTAATCATTGTAGACAGAGA+AGG 0.595043 5.2:+50420554 MS.gene73924:intron
GGTTAGTGCAAGTGGGTCTG+AGG 0.595559 5.2:+50420835 MS.gene73924:CDS
GGAACCACGATGGTTTGCTA+GGG 0.596524 5.2:+50424950 MS.gene73924:CDS
GGATAGAAATGGGAAAACAT+TGG 0.596727 5.2:+50422657 MS.gene73924:CDS
GTAGACAGAGAAGGTTGACC+TGG 0.597103 5.2:+50420563 MS.gene73924:intron
TGAAATATTCCAGTGGAGCA+AGG 0.598119 5.2:+50422263 MS.gene73924:CDS
TGAATTATGGTAAAGGATGG+AGG 0.598506 5.2:+50418211 MS.gene73924:CDS
AGTGACATGCAATTATCATA+GGG 0.599296 5.2:-50422128 None:intergenic
TACGGCTAGAGCAGCGACAA+CGG 0.603059 5.2:+50423219 MS.gene73924:CDS
CTCGTGATGATCGAGTGGCA+TGG 0.604319 5.2:+50419299 MS.gene73924:CDS
GTTACGATTGATGATACGCA+TGG 0.604717 5.2:-50418349 None:intergenic
CCGCGGAAGTCAGCTCGCGG+TGG 0.607570 5.2:+50418116 MS.gene73924:CDS
GGAAGATTCGGAGCGAGATG+AGG 0.607636 5.2:-50418055 None:intergenic
TTGATGAGAGCCAAAGACAA+TGG 0.608013 5.2:+50420598 MS.gene73924:CDS
GATTAAGGATAATATTGGGA+AGG 0.610369 5.2:+50421123 MS.gene73924:CDS
CCAGGTTAAGACCAGATCAA+AGG 0.610809 5.2:+50423153 MS.gene73924:CDS
TCATCATGGAAGAAGCCTGT+TGG 0.611229 5.2:-50420626 None:intergenic
AACAGGCTTCTTCCATGATG+AGG 0.612253 5.2:+50420628 MS.gene73924:CDS
AGCGGTACCTACCGCTACAT+AGG 0.612506 5.2:+50424981 MS.gene73924:CDS
TCAAAGGTATTTGGAAAATG+GGG 0.616103 5.2:+50423169 MS.gene73924:CDS
CCCAATATCCTCCTATGTAG+CGG 0.617010 5.2:-50424992 None:intergenic
CATCTTTGAAGAGAGGAGAG+AGG 0.617333 5.2:-50421184 None:intergenic
GGTACCTACCGCTACATAGG+AGG 0.618361 5.2:+50424984 MS.gene73924:CDS
ACATATAAAAGTACCTGGTG+AGG 0.620103 5.2:-50422508 None:intergenic
ATATCCTCCTATGTAGCGGT+AGG 0.621540 5.2:-50424988 None:intergenic
CAGCCGCGGAAGTCAGCTCG+CGG 0.624216 5.2:+50418113 MS.gene73924:CDS
GTCACTGTGAGGGTACAGGT+TGG 0.625184 5.2:+50422145 MS.gene73924:CDS
TATTGGGAAGCCCGACAACA+AGG 0.628212 5.2:+50425008 MS.gene73924:CDS
ATGAGCGGGAGATTGATGAT+CGG 0.628453 5.2:-50418004 None:intergenic
TGATGATCGAGTGGCATGGG+TGG 0.629154 5.2:+50419303 MS.gene73924:CDS
GAGAGCCAAAGACAATGGAA+TGG 0.630637 5.2:+50420603 MS.gene73924:CDS
ATCCAGAGGTTAGTGCAAGT+GGG 0.638560 5.2:+50420828 MS.gene73924:intron
TATTTAGTAGGAAACTTGGG+AGG 0.640925 5.2:-50422796 None:intergenic
GGTCTTAACCTGGAATCCGT+AGG 0.642698 5.2:-50423143 None:intergenic
CTTAACCTGGAATCCGTAGG+TGG 0.643105 5.2:-50423140 None:intergenic
TGAGACTCGTGATGATCGAG+TGG 0.643755 5.2:+50419294 MS.gene73924:CDS
CTTGACCGAGCTTATGAATG+GGG 0.646861 5.2:+50421235 MS.gene73924:CDS
CCACCGCGAGCTGACTTCCG+CGG 0.647447 5.2:-50418116 None:intergenic
GATCTCTGTAGTTGGATCCG+AGG 0.648525 5.2:+50420988 MS.gene73924:CDS
GCTCGGAAGATGCAAGAGAG+TGG 0.652263 5.2:+50424924 MS.gene73924:CDS
ATGGGTGGAGGCTTTGTATG+CGG 0.654208 5.2:+50419318 MS.gene73924:CDS
AGGAGGCATGGAGAGATGAG+CGG 0.654451 5.2:-50418019 None:intergenic
CTCTGTAGTTGGATCCGAGG+GGG 0.656234 5.2:+50420991 MS.gene73924:CDS
GACATGCAATTATCATAGGG+TGG 0.660760 5.2:-50422125 None:intergenic
AGAGTGGTTGGGAACCACGA+TGG 0.662475 5.2:+50424940 MS.gene73924:CDS
AGTACAGACCTTTAATCCTG+GGG 0.674406 5.2:-50422857 None:intergenic
ATAATTGCATGTCACTGTGA+GGG 0.675175 5.2:+50422135 MS.gene73924:CDS
GTTAGCGGAGATTGGTACGG+AGG 0.678256 5.2:-50418076 None:intergenic
AGCTCGCTGATGGACATCCG+AGG 0.682171 5.2:-50419355 None:intergenic
AGATTGATGATCGGAGACTG+CGG 0.689607 5.2:-50417995 None:intergenic
GTACCAATCTCCGCTAACGG+AGG 0.697880 5.2:+50418081 MS.gene73924:CDS
TCTCTGTAGTTGGATCCGAG+GGG 0.707988 5.2:+50420990 MS.gene73924:CDS
ATGATGGTGAAATATTCCAG+TGG 0.713311 5.2:+50422256 MS.gene73924:CDS
GAGGTTCATTAAAATAAACA+GGG 0.715478 5.2:-50421165 None:intergenic
GATAATTGCATGTCACTGTG+AGG 0.715730 5.2:+50422134 MS.gene73924:CDS
TTTGCTAGGGATAAAACAAG+CGG 0.731198 5.2:+50424963 MS.gene73924:CDS
TTATCCCTAGCAAACCATCG+TGG 0.748499 5.2:-50424954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.2 gene 50417973 50425109 50417973 ID=MS.gene73924
chr5.2 mRNA 50417973 50425109 50417973 ID=MS.gene73924.t1;Parent=MS.gene73924
chr5.2 exon 50417973 50418428 50417973 ID=MS.gene73924.t1.exon1;Parent=MS.gene73924.t1
chr5.2 CDS 50417973 50418428 50417973 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50419214 50419570 50419214 ID=MS.gene73924.t1.exon2;Parent=MS.gene73924.t1
chr5.2 CDS 50419214 50419570 50419214 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50420567 50420666 50420567 ID=MS.gene73924.t1.exon3;Parent=MS.gene73924.t1
chr5.2 CDS 50420567 50420666 50420567 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50420834 50421264 50420834 ID=MS.gene73924.t1.exon4;Parent=MS.gene73924.t1
chr5.2 CDS 50420834 50421264 50420834 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50421367 50421522 50421367 ID=MS.gene73924.t1.exon5;Parent=MS.gene73924.t1
chr5.2 CDS 50421367 50421522 50421367 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50422117 50422284 50422117 ID=MS.gene73924.t1.exon6;Parent=MS.gene73924.t1
chr5.2 CDS 50422117 50422284 50422117 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50422376 50422516 50422376 ID=MS.gene73924.t1.exon7;Parent=MS.gene73924.t1
chr5.2 CDS 50422376 50422516 50422376 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50422640 50422870 50422640 ID=MS.gene73924.t1.exon8;Parent=MS.gene73924.t1
chr5.2 CDS 50422640 50422870 50422640 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50423131 50423244 50423131 ID=MS.gene73924.t1.exon9;Parent=MS.gene73924.t1
chr5.2 CDS 50423131 50423244 50423131 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
chr5.2 exon 50424924 50425109 50424924 ID=MS.gene73924.t1.exon10;Parent=MS.gene73924.t1
chr5.2 CDS 50424924 50425109 50424924 ID=cds.MS.gene73924.t1;Parent=MS.gene73924.t1
Gene Sequence

>MS.gene73924

ATGCATCCTTTCTGCTGCATCTCCGCAGTCTCCGATCATCAATCTCCCGCTCATCTCTCCATGCCTCCTCTTCCTTCCTCCACCTCATCTCGCTCCGAATCTTCCTCCGTACCAATCTCCGCTAACGGAGGAGGTGATTGCAGCCGCGGAAGTCAGCTCGCGGTGGCTCATACGGCGGTTGACGTTAGGATCAACGACCTCGTCGGGAATGGAATATCTGGAATACTTCAGAAATGGGTGAATTATGGTAAAGGATGGAGGCATCGATGGTTCGTGTTGCAAGATGGTGTTCTTTCTTATTACAAGATTCATGGACCTGATAAGATTGTAATCAATCCTGAAACTGAGAAAGGATTCAAGGTTATTGGTGATGAATCCATGCGTATCATCAATCGTAACAAAAATTCTCATCACTCTCAAAATTGGCGTAAGCCTTTCGGAGAAATTCACCTCAAGGTTCATGCATTAACCGTTGTTCTTGATTCGTTTTTAGTTTTAATTCTGTTGTTGTTGTTGTTGTTGTTTGCAAGAGGAATTTGAGTTTGGATGTTGAATTAATTAATTAATCAAGCTTCAATTGTATCTGAACCTGAATAAATGAGTTATCTACTTTTGATTTTGATGGAAGGCGTGTCCGGTGTCTGACACATGTGATTACATTCAATTAAGGGTCTGTTTGGATAAAACAGCTTATTTGCAGCTTGTAGCACAGGCGCCTATGTATAGGCTTACATAAGCTATGTTTATAGTAAAAGATTAAATAAAGTTAAATTGTTTTTGTAAACGCTATAAGCTATTTTTATAAGTTATGTCGGAGAACTTATGAAAATAAATTGAATGAAGGAAGCATATAAAAATTGTCATGAGCTGTTTTCAGATGTTCTCCTTATGCCAGTTGATAAAGCTCAAATAAGCCAATCCAAACCTAATTCATTTTTTTTTTCCAACTTTTTACAATGTGTCTTGTGTCATATCTGGTGTCGGTGTTGTGTCATATCCGGTGTCCGTGTCTATGTCAGTGCTTCATTGATCTTAGGTTGTTGATTTCTTCTTCTTCTTCTTCTGTGTCCTGATTTCAAGATGCAATTAGCTATTAATGAATCATATGAGGATGTGATATTAATATATGGCATGATATATAATTGATGTGGCTCTGAAATTAGGGAATCCAAATTTTATTTTCATAAGATAATATCAATATTTATGTGTAGTATTTTACGGGAAGAAAGGTTTGATTGTAGGTTTCAACTATTCGCGAGAGCAGATCCGATGATAAACGGTTTTCCATTTTCACCGGGACAAAGACACTGCACTTGAGAGCTGAGACTCGTGATGATCGAGTGGCATGGGTGGAGGCTTTGTATGCGGTCAAAGATATGTTTCCTCGGATGTCCATCAGCGAGCTGATGGCTCCAGTGGACAATACGGCCGTTTCAACTGAAAAGCTAAGGCATCGGCTTATGGAAGAAGGTGTTAGTGAAGAAGCTATTCAGGATAGCGAGCAAATTATGAGGAATGAGTATGCTGCTCTACAAAACCAGCTTTTGCTACTTAAGCAGAAGCAGTTGGCACTAATCGACAATTTACGCCATTTGGAGGTACATTTAATGAAAAGTAGAACACTTCATGCCAATCAAACTGGCTAATTTTGTTTGAATTCTGCTCCGCGGAATGCTGGTCTTAAATACCGTATATAATTATCAGCAAGAAGGGGGGTATGCTGCCTTTTTTTTTTCATTTAAAAGAAAGTAGAGAATCGTATATTAGCCTGTACATTTGTTAACTATAGTTTTTCCCCATTTTCTGAGCTGTACAATTGTATGTTCGCAATTAAAAGCCAAAAACTAATGGCTTTAATTAATAATATTCAGTATTCTTTGGTCAATTTCTCCCCCCTTTACTTTCCCATTGGTTTTAGCAAACAATTAGTCCTATAGCCTGTATTCAGAATCACTTCTTTACTAATTAGATCATCCTTCAAATAGGGTTTCGGTGTCCCTAAGATATAAATTTTAAATCAGAAAAATGAAGCTCTAGTTAGTGTGTTTATACTAAGTCTAAATGATCTTTTTATTTTTTCATTTAATCCTTATGATAATTTTTGAACATAACAGCAGCCAAATACTATTTGGAAATTATTCCGTCAACTTATTATGAACAAATTAATGTTAAGTGTCGTTATAAAAATTTGTATAAGGCTGAAAAGAGACACTGCTGAGATATATTTATGGGTTTCGTTTGTTTATCTTAACGGAGAAGTCTTTCAGCAATTGGCAAAATAATAAAATACTATACCTAAATTTGAGTTATCTGATATTAATTTTAGCCAAAAATTTTGTGCGCTTTCAGATAGCCCATTATGAATTAATGTGTTTTGCAAATGCAATTCCTTACCTTGCTGACCACTTCCAATTACTGTCTTAATATTTCTTTTTAAGCAAAAAATGGTTTTCCCTCATTAAAATTCTAGGAGTGGGTTTTTGTTTGATCCAGTTTTTTTTGTTGCTTCTTTATATTTATTGACATATTGATTAAGGCTAATATTTTGTTTTCCTAATGTAGTACTAAAATTTATTTGTACTTAATCATTGTAGACAGAGAAGGTTGACCTGGAAAACACTGTTGTTGATGAGAGCCAAAGACAATGGAATGGCCAACAGGCTTCTTCCATGATGAGGCAGGAAAAGTTTAGTGGTAAGTGGTTGAATATTCAGCCATTCATAAATTTAAGTACTTTTAGCTGCTCATTTTTTGTTTTGCTTATGCATTTTCTTAAAAAACTAGAAGTACTTCCATGCCAGCTATTATATTTAATACACTGATTTTCTATGTACTGTTGCGATTTTCAATTCTTAATCCAGAGGTTAGTGCAAGTGGGTCTGAGGATGATAATGAGAGAAATGATGCAGTAGAGGAAGAAACAGATGATGATGATACCACCTTTTTTGACACCCGTGATTTTATATCATCATCCAGTTCTGTTAGGGCTTCATCCTTTTCTTCAGATGATGAAGGGATCTCTGTAGTTGGATCCGAGGGGGATATAGATCCTTCTATTAGATCTGTTGGGACCAACTACCCCCACGTCAAGCGGCGGAAAAAATTGCCTGAACCTGTTGAAAAAGAGAAAGGTGTCAGTCTTTGGTCATTGATTAAGGATAATATTGGGAAGGATCTAACTAAAGTATGTCTCCCTGTTTATTTTAATGAACCTCTCTCCTCTCTTCAAAGATGCTGTGAAGAAATGGAGTACTCATATCTCCTTGACCGAGCTTATGAATGGGGAAAAAGAGTAAGTCTGGATTTAGTACCCAAGTTTCACAATGTTTATTGCTTCAGTGTATTGCTGAAAACAATGTTTCAATTTTTTAAAACCTTTGATTATGTTGTTCAGGGCAATACGCTTATGAGGATTCTCAATGTTGCTGCTTTTGCTGTATCTGCCTATGCTTCAACTGATGGGAGAAATTGTAAACCGTTTAATCCATTACTGGGCGAGACATATGAGGCTGAATATCCAGATAAAGGTTTTCGTTTCATCTCAGAGAAGGTCTAATCTTAGTCTACACAATGATATTATTAATACACAATCTTTCGTTGTGATTATGTTGATTGTATGTGTTAACGATGTACTATTTGACACCTTAGAACAATTTCATTAGAAGTGAAAGTTTCTTATTGTTGTTTTGGGGAGGGGAGGTCGCCATGTTTCAGGGATAGGTAATGCCACAAGCCTTACTATGTCTTTAGCATTATACTTTATAATAACCTGTCATTGATGGACTACCAGACAGAATTGAAAAGGTGTGAGCATGTGACTCTGTTATTCGTGGTAATTATATGATGTATGTATTTAAGTTTTTATTTGTTGAAATTCCCATGCATAAGTAGTTATGTTTATTTGAGTTCCTTAGAACTTCTTGCTCCAGGATGAAAAGGAAACAGTTTTGAGGAAGAACTGAAGTAGAATGGTAGATACCCAACTTAATTTGATGAATAGAAATTCATGTGGTTGTGTGCTTAAGTTCCATAGAACTCTATAAAATACGATAAATCCTGATTTGTATTTAACAAGTGATATGATTGTTTATGCAAACACTATTTGCATTATGAATTTAAAATACTTATTTCTGCTTCCTCTCAGGTCAGTCACCACCCTATGATAATTGCATGTCACTGTGAGGGTACAGGTTGGAAATTTTGGGCAGATAGCAACTTAAAGAGCAAATTTTGGGGCCGATCAATTCAACTTGATCCTGTTGGTATTTTGACATTAGAATTTGATGATGGTGAAATATTCCAGTGGAGCAAGGTACATTTAACTTCTTGCACTATTATGTGTTGAATGCCACAAAGAAAATGCTATACCCTGATTTGGTTCTAAATTGGAACTGTTCTTTCAGGTTACTACATCAATATACAATATCATTTTGGGAAAGCTGTATTTGGATCATTATGGTACAATGCGCATTCAGGGAAACCAAGAATACTCATGTAAACTGAAATTCAAGGAACAATCTATGATTGATCGCAATCCTCACCAGGTACTTTTATATGTTTTACAGATTATGTCTTTGCAACAATTAACTTTTGTGGAATATAGTACTCGACATGTGATCCTTTATTGTATATGATAGTTTCTAATTGCAGTTTGTCTGTCTTTGCAGGTTCAAGGTACTGTTCAGGATAGAAATGGGAAAACATTGGCTACATTAATGGGAAAGTGGAATGACAGCATGTATTATGTTAATGGAGACTATGGTGGAAAGGGGAAAGGTTATGAATCTCTGCCAGAAGCGCAAATACTATGGAAGCGGAGCAAGCCTCCCAAGTTTCCTACTAAATATAACTTAACTCGTTTTGCCATCACATTAAATGAACTTACCCCAGGATTAAAGGTCTGTACTTTTAACTGATCCTTTATTGTGTTTCCAATCTGCGTGTTTAGTTTTTACTTTTTATATTTATTTTTTAAAGGAAAAATTTGCTATGTTTTTCACTGTCATCTTTTACCACATGAGAAAATTGTTACTTGCATTAGGACAGGAACCTTTTGACTATCTCTATAACATGGCAGAGCTTTAAAGTATAAGATATTGTGCATGTATCTCATTACCTGGAATTGGACATTGATTATCAATTTTATTTGAGCATATAGGAAAAGCTGCCACCTACGGATTCCAGGTTAAGACCAGATCAAAGGTATTTGGAAAATGGGGAGTATGAGATGGGAAATTCAGAAAAATTACGGCTAGAGCAGCGACAACGGCAGGTAGATATTTCATTTTATGTTATGCATTTCATTTGTTTGTTTCACTTGTAGGGTAGCCTTAAGTGTGCGTTTGGTCAACATGCTAGAAGGCCAAAAAACAAATGAGAAAGGCGTGAGATTTAATAATTGGTACACTTTGGTTCCATGAAAATGGATAAGAAATGAAGAATATACCAAACCTTTTAGTCTCATTCTATCTGATTTTATTTTGCTCACGAACCAAGCTCTATATGAGAGAGGGGAGCCTAGTGGGTGAGTTACTTAGAAAATTCATATCATTTTAGATTTCCTTAAATCTTACTGCGATAAGATTTAAGACTAGGAAACATATTTAGCACTGAGCCCTTCCCATAAGCAAGACCTTATTGTCTGACCTTTTAACAGATATTTTTGACGAAGTTTCCCACAATGTGGTGTTTCATCTACTCATTAGAACGTATGAATTTAACATGCCATTCCTTGTAGACCAAGCAATACAAGTCTACATAAAATTATGAAGTAGTAAATAGGAAATATCCATAAATGCTGCTTATTACATGGCTAGAGAAGTTTTAAACTTTCAAAACATTTTATACGTTCTCCTGATTCTCAATGTTCTTATTTTGATTGTTTATTCAAATTCTAGTATTTCCACCATCCATGCGAAAAGAGAAATGAAAGATGTATTTCAGATATCAGCATTGTATGTCCTTTGTCTGTTCTGCTGGAAATCTACATTTTATTTTTAACTGATGTAAATATTTTTTGGTCCACTAAAATAAGTTTATTATTGCCTAGTCTAAAAAATTTAGTCAGCTTTTGGTCCCTCTTTTTTGAGTGAGAGAGACCAATTGTGATCATTTCCACAAAGAGTAACCAAAAGGTAACAATTTTTTGCATGGAGATGTAAACAATAGTCACTGTATTTTGAATTACTAAAAGCATCTTTATGGCTTTTTTAATTTGCTGCAACACAGCAAAAAATTTCTTAGTTTGAATTACTGAACTAAGATATGCTGAAATGTTCCCCTGTTACTTAACACCAAGTACTCTTGTGAACAAAATATTAAGAGAAAAACTTAACAGAGGAACACTTTACAATATCTCTGTTTTAACTGTAATGCCTTGATCTCTAGCCTTGAGTACATTTCATATCTGTAGATACGACATTGTAGGGGATCTGATCTTTTTAATAATTATGCACTCAAACTGTAAATTGACTACATGTCTGTTCTAATGCTACCTACTAAAATATCTAAAATTAATCAACATGATTTTTTTTTTTTTTTTGATAGAGATATTTGCATCTATGTAAAACATTTTTACACCATCAATATGTAGTAATTTAATTATTATCGCGGAAGTAAAATATATCTGGGTTAAGTATATCTGTACACTCAAAATATGAAGTTACTGTTGTTTTAATCCTATTCAAATTTTATTTGATTTTAGTTGTTCCATATAAAACAAAAATTGTTACATATAGCAAAAATGATACTATATTTCTGACAAAAAAAAAAAAGGTACTATATTTTAGTCCTAATTAGCGTTTGAAAACATGCTTTCTATAGTTTTTCATAGTAATAAGTAAAATTTAAAACAAAATCATAATCCAAATTTGCTAATTTGATACAACTACAACATATTTAATCCATGTTTCTTGTCCTTGCTGATTAAACACTTGTTTCATTTTGATTTAGGCTCGGAAGATGCAAGAGAGTGGTTGGGAACCACGATGGTTTGCTAGGGATAAAACAAGCGGTACCTACCGCTACATAGGAGGATATTGGGAAGCCCGACAACAAGGAAGTTGGAACTCTTGTCCTAATATTTTTGGTCAAATTCCTTCTGAGCATATTTTAGACGAAGGTTCAACTACATTGTAG
Protein sequence

>MS.gene73924.t1

MHPFCCISAVSDHQSPAHLSMPPLPSSTSSRSESSSVPISANGGGDCSRGSQLAVAHTAVDVRINDLVGNGISGILQKWVNYGKGWRHRWFVLQDGVLSYYKIHGPDKIVINPETEKGFKVIGDESMRIINRNKNSHHSQNWRKPFGEIHLKVSTIRESRSDDKRFSIFTGTKTLHLRAETRDDRVAWVEALYAVKDMFPRMSISELMAPVDNTAVSTEKLRHRLMEEGVSEEAIQDSEQIMRNEYAALQNQLLLLKQKQLALIDNLRHLETEKVDLENTVVDESQRQWNGQQASSMMRQEKFSEVSASGSEDDNERNDAVEEETDDDDTTFFDTRDFISSSSSVRASSFSSDDEGISVVGSEGDIDPSIRSVGTNYPHVKRRKKLPEPVEKEKGVSLWSLIKDNIGKDLTKVCLPVYFNEPLSSLQRCCEEMEYSYLLDRAYEWGKRGNTLMRILNVAAFAVSAYASTDGRNCKPFNPLLGETYEAEYPDKGFRFISEKVSHHPMIIACHCEGTGWKFWADSNLKSKFWGRSIQLDPVGILTLEFDDGEIFQWSKVTTSIYNIILGKLYLDHYGTMRIQGNQEYSCKLKFKEQSMIDRNPHQVQGTVQDRNGKTLATLMGKWNDSMYYVNGDYGGKGKGYESLPEAQILWKRSKPPKFPTKYNLTRFAITLNELTPGLKEKLPPTDSRLRPDQRYLENGEYEMGNSEKLRLEQRQRQARKMQESGWEPRWFARDKTSGTYRYIGGYWEARQQGSWNSCPNIFGQIPSEHILDEGSTTL