Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94096.t1 | XP_013453992.2 | 99.1 | 779 | 7 | 0 | 1 | 779 | 1 | 779 | 0.00E+00 | 1434.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94096.t1 | Q8L751 | 70.5 | 814 | 200 | 7 | 1 | 774 | 1 | 814 | 7.2e-301 | 1034.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94096.t1 | A0A396HTZ2 | 99.1 | 779 | 7 | 0 | 1 | 779 | 1 | 779 | 0.0e+00 | 1434.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067071 | MS.gene94096 | PPI |
| MS.gene30772 | MS.gene94096 | PPI |
| MS.gene94096 | MS.gene053748 | PPI |
| MS.gene94096 | MS.gene75538 | PPI |
| MS.gene29058 | MS.gene94096 | PPI |
| MS.gene94096 | MS.gene045181 | PPI |
| MS.gene94096 | MS.gene031848 | PPI |
| MS.gene94096 | MS.gene73924 | PPI |
| MS.gene94096 | MS.gene35664 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94096.t1 | MTR_5g459350 | 98.946 | 759 | 8 | 0 | 21 | 779 | 1 | 759 | 0.0 | 1567 |
| MS.gene94096.t1 | MTR_3g073040 | 76.981 | 795 | 155 | 7 | 1 | 778 | 1 | 784 | 0.0 | 1246 |
| MS.gene94096.t1 | MTR_3g073040 | 76.855 | 795 | 155 | 8 | 1 | 778 | 1 | 783 | 0.0 | 1239 |
| MS.gene94096.t1 | MTR_3g097270 | 61.104 | 761 | 268 | 8 | 11 | 769 | 15 | 749 | 0.0 | 952 |
| MS.gene94096.t1 | MTR_3g073040 | 76.329 | 621 | 119 | 7 | 1 | 604 | 1 | 610 | 0.0 | 945 |
| MS.gene94096.t1 | MTR_3g086340 | 56.473 | 811 | 298 | 15 | 1 | 765 | 1 | 802 | 0.0 | 891 |
| MS.gene94096.t1 | MTR_4g101410 | 54.304 | 790 | 314 | 13 | 1 | 773 | 6 | 765 | 0.0 | 830 |
| MS.gene94096.t1 | MTR_3g086340 | 54.977 | 653 | 244 | 11 | 1 | 609 | 1 | 647 | 0.0 | 692 |
| MS.gene94096.t1 | MTR_4g101410 | 61.338 | 538 | 198 | 5 | 242 | 773 | 1 | 534 | 0.0 | 674 |
| MS.gene94096.t1 | MTR_7g061830 | 34.037 | 379 | 222 | 13 | 397 | 759 | 73 | 439 | 4.54e-48 | 177 |
| MS.gene94096.t1 | MTR_4g064110 | 32.294 | 449 | 243 | 21 | 334 | 751 | 9 | 427 | 1.75e-45 | 169 |
| MS.gene94096.t1 | MTR_4g115490 | 44.199 | 181 | 26 | 4 | 304 | 482 | 43 | 150 | 7.09e-28 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94096.t1 | AT4G08180 | 69.475 | 819 | 200 | 9 | 1 | 774 | 1 | 814 | 0.0 | 1123 |
| MS.gene94096.t1 | AT4G08180 | 69.475 | 819 | 200 | 9 | 1 | 774 | 1 | 814 | 0.0 | 1123 |
| MS.gene94096.t1 | AT4G08180 | 69.353 | 819 | 200 | 10 | 1 | 774 | 1 | 813 | 0.0 | 1117 |
| MS.gene94096.t1 | AT4G08180 | 68.987 | 819 | 203 | 10 | 1 | 774 | 1 | 813 | 0.0 | 1110 |
| MS.gene94096.t1 | AT2G31020 | 68.216 | 796 | 200 | 10 | 1 | 766 | 1 | 773 | 0.0 | 1047 |
| MS.gene94096.t1 | AT1G13170 | 58.209 | 804 | 289 | 13 | 1 | 763 | 1 | 798 | 0.0 | 919 |
| MS.gene94096.t1 | AT1G13170 | 56.727 | 825 | 289 | 14 | 1 | 763 | 1 | 819 | 0.0 | 907 |
| MS.gene94096.t1 | AT4G22540 | 51.684 | 772 | 309 | 9 | 1 | 769 | 6 | 716 | 0.0 | 808 |
| MS.gene94096.t1 | AT4G22540 | 51.684 | 772 | 309 | 9 | 1 | 769 | 6 | 716 | 0.0 | 808 |
| MS.gene94096.t1 | AT4G12460 | 53.805 | 749 | 271 | 13 | 32 | 769 | 4 | 688 | 0.0 | 803 |
| MS.gene94096.t1 | AT4G12460 | 53.805 | 749 | 271 | 13 | 32 | 769 | 4 | 688 | 0.0 | 803 |
| MS.gene94096.t1 | AT4G12460 | 53.733 | 750 | 271 | 14 | 32 | 769 | 4 | 689 | 0.0 | 799 |
| MS.gene94096.t1 | AT2G31030 | 77.531 | 405 | 84 | 2 | 362 | 766 | 91 | 488 | 0.0 | 681 |
| MS.gene94096.t1 | AT4G12460 | 51.970 | 660 | 242 | 13 | 32 | 680 | 4 | 599 | 0.0 | 674 |
| MS.gene94096.t1 | AT4G12460 | 51.891 | 661 | 242 | 14 | 32 | 680 | 4 | 600 | 0.0 | 669 |
| MS.gene94096.t1 | AT4G22540 | 57.778 | 540 | 193 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene94096.t1 | AT4G22540 | 57.778 | 540 | 193 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene94096.t1 | AT4G22540 | 57.778 | 540 | 193 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene94096.t1 | AT4G22540 | 57.778 | 540 | 193 | 4 | 230 | 769 | 1 | 505 | 0.0 | 657 |
| MS.gene94096.t1 | AT4G12460 | 58.519 | 540 | 184 | 5 | 230 | 769 | 1 | 500 | 0.0 | 655 |
| MS.gene94096.t1 | AT4G12460 | 58.519 | 540 | 184 | 5 | 230 | 769 | 1 | 500 | 0.0 | 655 |
| MS.gene94096.t1 | AT4G12460 | 58.519 | 540 | 184 | 5 | 230 | 769 | 1 | 500 | 0.0 | 655 |
| MS.gene94096.t1 | AT4G12460 | 69.919 | 369 | 102 | 2 | 401 | 769 | 1 | 360 | 0.0 | 563 |
| MS.gene94096.t1 | AT1G77730 | 48.462 | 260 | 118 | 1 | 36 | 279 | 2 | 261 | 1.45e-76 | 248 |
| MS.gene94096.t1 | AT3G09300 | 36.119 | 371 | 205 | 16 | 399 | 751 | 75 | 431 | 1.09e-50 | 184 |
| MS.gene94096.t1 | AT5G02100 | 34.367 | 387 | 220 | 14 | 355 | 732 | 11 | 372 | 1.59e-48 | 177 |
| MS.gene94096.t1 | AT5G02100 | 34.367 | 387 | 220 | 14 | 355 | 732 | 38 | 399 | 2.47e-48 | 177 |
| MS.gene94096.t1 | AT5G59420 | 34.409 | 372 | 214 | 14 | 397 | 751 | 68 | 426 | 1.53e-45 | 170 |
| MS.gene94096.t1 | AT5G02100 | 34.050 | 279 | 162 | 9 | 355 | 629 | 38 | 298 | 1.80e-34 | 134 |
Find 219 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAATATACAATATCATTT+TGG | 0.118580 | 5.4:+46481959 | MS.gene94096:CDS |
| TTGAATCCTTTCTCAGTTTC+AGG | 0.126035 | 5.4:-46477903 | None:intergenic |
| GCAACTTAAAGAGCAAATTT+TGG | 0.166489 | 5.4:+46481759 | MS.gene94096:CDS |
| GTTGGCCATTCCATTGTCTT+TGG | 0.203387 | 5.4:-46480186 | None:intergenic |
| AGGGTACAGGTTGGAAATTT+TGG | 0.226618 | 5.4:+46481729 | MS.gene94096:CDS |
| GAGAAAGGATTCAAGGTTAT+TGG | 0.235224 | 5.4:+46477912 | MS.gene94096:CDS |
| CAGTCTTTGGTCATTGATTA+AGG | 0.238789 | 5.4:+46480687 | MS.gene94096:CDS |
| GATCAGAGGTATTTGGAAAA+TGG | 0.239645 | 5.4:+46482735 | MS.gene94096:CDS |
| GAAAACATTGGCTACATTAA+TGG | 0.241182 | 5.4:+46482245 | MS.gene94096:CDS |
| ATTCTCATCACTCTCAAAAT+TGG | 0.248288 | 5.4:+46477970 | MS.gene94096:CDS |
| TCAGTTTACATGAGTATTCT+TGG | 0.255621 | 5.4:-46482028 | None:intergenic |
| CTTCAGAAATGGGTGAATTA+TGG | 0.278794 | 5.4:+46477792 | MS.gene94096:CDS |
| GACAGCATGTATTATGTTAA+TGG | 0.279631 | 5.4:+46482279 | MS.gene94096:CDS |
| AAGACCAGATCAGAGGTATT+TGG | 0.284475 | 5.4:+46482728 | MS.gene94096:CDS |
| TGAACTTACCCCAGGATTAA+AGG | 0.295249 | 5.4:+46482425 | MS.gene94096:CDS |
| CTTTCTTATTACAAGATTCA+TGG | 0.296312 | 5.4:+46477858 | MS.gene94096:CDS |
| TGTCTTTGTCCCAGTGAAAA+TGG | 0.299157 | 5.4:-46478819 | None:intergenic |
| TATCATCATCCAGTTCTGTT+AGG | 0.301667 | 5.4:+46480515 | MS.gene94096:CDS |
| TTCCATAGTATTTGTGCTTC+TGG | 0.306780 | 5.4:-46482339 | None:intergenic |
| AGAGGTTCATTAAAATAAAC+AGG | 0.322549 | 5.4:-46480745 | None:intergenic |
| AAAGCTAAGGCATCGGCTTA+TGG | 0.329463 | 5.4:+46478976 | MS.gene94096:CDS |
| ATGAAACGAAAACCTTTATC+TGG | 0.331208 | 5.4:-46481069 | None:intergenic |
| TCATTGATTAAGGATAATAT+TGG | 0.334852 | 5.4:+46480697 | MS.gene94096:CDS |
| AATCGACAATTTACGCCATT+TGG | 0.342761 | 5.4:+46479108 | MS.gene94096:CDS |
| TGTTAGTGAAGAAGCAATTC+AGG | 0.350937 | 5.4:+46479006 | MS.gene94096:CDS |
| AAGCTGTATTTGGATCATTA+TGG | 0.351321 | 5.4:+46481984 | MS.gene94096:CDS |
| CAGTGACATGCAACTATCAT+AGG | 0.356777 | 5.4:-46481704 | None:intergenic |
| GGATTGATTACAATCTTATC+AGG | 0.360001 | 5.4:-46477882 | None:intergenic |
| ACCGCTACGTAGGAGGATAT+TGG | 0.362491 | 5.4:+46484331 | MS.gene94096:CDS |
| TATTATGTTAATGGAGAATA+TGG | 0.368929 | 5.4:+46482288 | MS.gene94096:CDS |
| AAAACATTGGCTACATTAAT+GGG | 0.370877 | 5.4:+46482246 | MS.gene94096:CDS |
| TGCCAGAAGCACAAATACTA+TGG | 0.373666 | 5.4:+46482337 | MS.gene94096:CDS |
| AAGAGAAAGGTGTCAGTCTT+TGG | 0.374372 | 5.4:+46480674 | MS.gene94096:CDS |
| TATCTGGAATACTTCAGAAA+TGG | 0.374717 | 5.4:+46477781 | MS.gene94096:CDS |
| TAAACCGTTTAATCCATTAC+TGG | 0.378853 | 5.4:+46481023 | MS.gene94096:CDS |
| TCAAAGATGCTGTGAAGAAA+TGG | 0.381841 | 5.4:+46480777 | MS.gene94096:CDS |
| CAAAATTGGCGTAAGCCTTT+CGG | 0.382485 | 5.4:+46477984 | MS.gene94096:CDS |
| ATCAGAGGTATTTGGAAAAT+GGG | 0.383734 | 5.4:+46482736 | MS.gene94096:CDS |
| CTCGTTGGGAATGGAATATC+TGG | 0.384420 | 5.4:+46477765 | MS.gene94096:CDS |
| ATGAGTATTCTTGGTTTCCC+TGG | 0.384839 | 5.4:-46482019 | None:intergenic |
| TGTCTGTCTTTGCAGGTTCA+AGG | 0.396316 | 5.4:+46482201 | MS.gene94096:intron |
| GATCCTTCTATTAGATCTGT+TGG | 0.397837 | 5.4:+46480598 | MS.gene94096:CDS |
| GGTACTGTTCAGGATAGAAA+TGG | 0.403022 | 5.4:+46482222 | MS.gene94096:CDS |
| TCAGGATCAACGACCTCGTT+GGG | 0.404469 | 5.4:+46477751 | MS.gene94096:CDS |
| GATTGGTACGGAGGAAGATT+CGG | 0.417845 | 5.4:-46477661 | None:intergenic |
| CCGCTACGTAGGAGGATATT+GGG | 0.418285 | 5.4:+46484332 | MS.gene94096:CDS |
| GAGGCTGAATATCCAGATAA+AGG | 0.418986 | 5.4:+46481057 | MS.gene94096:CDS |
| AGCTGCCACCTACAGATTCC+AGG | 0.420280 | 5.4:+46482703 | MS.gene94096:CDS |
| GCAGGTTCAAGGTACTGTTC+AGG | 0.421855 | 5.4:+46482212 | MS.gene94096:intron |
| GGAGGAAGGAAGAGGAGGCA+TGG | 0.425480 | 5.4:-46477625 | None:intergenic |
| ATCATCATCCAGTTCTGTTA+GGG | 0.428400 | 5.4:+46480516 | MS.gene94096:CDS |
| CATTGATTAAGGATAATATT+GGG | 0.435505 | 5.4:+46480698 | MS.gene94096:CDS |
| TCGCCCAGTAATGGATTAAA+CGG | 0.435757 | 5.4:-46481027 | None:intergenic |
| CCCTTCATCATCTGAAGAAA+AGG | 0.437910 | 5.4:-46480545 | None:intergenic |
| CAACATTGAGAATCCTCATA+AGG | 0.438158 | 5.4:-46480954 | None:intergenic |
| ATCTGGAATACTTCAGAAAT+GGG | 0.440833 | 5.4:+46477782 | MS.gene94096:CDS |
| CTCATACGGCGGTTGACGTC+AGG | 0.441790 | 5.4:+46477733 | MS.gene94096:CDS |
| TTAAATGTACCTTGCTCCAC+TGG | 0.443931 | 5.4:-46481847 | None:intergenic |
| TCAGCTCGCGGTGGCTCATA+CGG | 0.450923 | 5.4:+46477719 | MS.gene94096:CDS |
| GGATGAAGCCCTAACAGAAC+TGG | 0.451204 | 5.4:-46480524 | None:intergenic |
| ATTCCAACTTCCTTGCTGTC+GGG | 0.451772 | 5.4:-46484358 | None:intergenic |
| CCTCCTCCGTTAGCGGAGAT+TGG | 0.453401 | 5.4:-46477678 | None:intergenic |
| AATGGAGAATATGGTGGAAA+GGG | 0.457611 | 5.4:+46482297 | MS.gene94096:CDS |
| TCCTTGACCGAGCTTATGAA+TGG | 0.464437 | 5.4:+46480812 | MS.gene94096:CDS |
| GATGAGGTGGAGGAAGGAAG+AGG | 0.468545 | 5.4:-46477633 | None:intergenic |
| TCTAATGTCAAAATACCAAC+AGG | 0.470049 | 5.4:-46481803 | None:intergenic |
| GTCCCAACAGATCTAATAGA+AGG | 0.474628 | 5.4:-46480601 | None:intergenic |
| TCATTAAATGTACCTCCAAA+TGG | 0.475571 | 5.4:-46479123 | None:intergenic |
| AAGACAATGGAATGGCCAAC+AGG | 0.476443 | 5.4:+46480189 | MS.gene94096:CDS |
| TGAAACTGAGAAAGGATTCA+AGG | 0.478956 | 5.4:+46477905 | MS.gene94096:CDS |
| GGGAACCACGATGGTTTGCT+AGG | 0.480681 | 5.4:+46484289 | MS.gene94096:CDS |
| ATCCTTCTATTAGATCTGTT+GGG | 0.481357 | 5.4:+46480599 | MS.gene94096:CDS |
| GTTATATCTAGTAGGAAACT+TGG | 0.484791 | 5.4:-46482376 | None:intergenic |
| TTATGGTACAATGCGCATCC+AGG | 0.485935 | 5.4:+46482001 | MS.gene94096:CDS |
| CCTCTGATCTGGTCTTAACC+TGG | 0.487702 | 5.4:-46482721 | None:intergenic |
| CGACAATTTACGCCATTTGG+AGG | 0.488709 | 5.4:+46479111 | MS.gene94096:CDS |
| TTCAGGGCAATACCCTTATG+AGG | 0.490577 | 5.4:+46480941 | MS.gene94096:intron |
| CCTTGACCGAGCTTATGAAT+GGG | 0.490785 | 5.4:+46480813 | MS.gene94096:CDS |
| AAAGCACAGACCTTTAATCC+TGG | 0.493056 | 5.4:-46482435 | None:intergenic |
| CGAGTTAAGTTATATCTAGT+AGG | 0.495813 | 5.4:-46482384 | None:intergenic |
| GGAAGATGCAAGAGAGTGGT+TGG | 0.497056 | 5.4:+46484268 | MS.gene94096:CDS |
| ATCTCTGTAGTTGGATCCGA+GGG | 0.497057 | 5.4:+46480568 | MS.gene94096:CDS |
| AACATTGAGAATCCTCATAA+GGG | 0.497664 | 5.4:-46480953 | None:intergenic |
| GATGAAGGGATCTCTGTAGT+TGG | 0.498961 | 5.4:+46480559 | MS.gene94096:CDS |
| TGAGAGAAATGATGCAGTAG+AGG | 0.500636 | 5.4:+46480444 | MS.gene94096:CDS |
| AAGCACAGACCTTTAATCCT+GGG | 0.501026 | 5.4:-46482434 | None:intergenic |
| AATTCCAACTTCCTTGCTGT+CGG | 0.506969 | 5.4:-46484359 | None:intergenic |
| GGAGGCATGGAGAGATGAGC+GGG | 0.507571 | 5.4:-46477612 | None:intergenic |
| TCATATGTCTCGCCCAGTAA+TGG | 0.509403 | 5.4:-46481036 | None:intergenic |
| GGCTTCTTCCATGATGAGGC+AGG | 0.510248 | 5.4:+46480210 | MS.gene94096:CDS |
| AAATGGGTGAATTATGGTAA+AGG | 0.510547 | 5.4:+46477798 | MS.gene94096:CDS |
| TCTGCTTAAGTAGCAAAAGC+TGG | 0.512576 | 5.4:-46479072 | None:intergenic |
| GGAAAATGGGGAGTATGAGA+TGG | 0.513993 | 5.4:+46482749 | MS.gene94096:CDS |
| TCTGCCTATGCTTCAACTGA+TGG | 0.517519 | 5.4:+46480991 | MS.gene94096:CDS |
| ATCAACGACCTCGTTGGGAA+TGG | 0.517936 | 5.4:+46477756 | MS.gene94096:CDS |
| TTGACATTAGAATTTGATGA+TGG | 0.523339 | 5.4:+46481815 | MS.gene94096:CDS |
| CTCATGTAAACTGAAATTCA+AGG | 0.524169 | 5.4:+46482037 | MS.gene94096:CDS |
| TTCGGAGCGAGATGAGGTGG+AGG | 0.524235 | 5.4:-46477643 | None:intergenic |
| ATCAATCCTGAAACTGAGAA+AGG | 0.524425 | 5.4:+46477897 | MS.gene94096:CDS |
| CCAATCTCCGCTAACGGAGG+AGG | 0.524579 | 5.4:+46477678 | MS.gene94096:CDS |
| GGTAAGTTCATTTAATGTGA+TGG | 0.529011 | 5.4:-46482412 | None:intergenic |
| ATTACTGGGCGAGACATATG+AGG | 0.531058 | 5.4:+46481038 | MS.gene94096:CDS |
| CCCATTCATAAGCTCGGTCA+AGG | 0.532735 | 5.4:-46480813 | None:intergenic |
| GTACTGTTCAGGATAGAAAT+GGG | 0.533845 | 5.4:+46482223 | MS.gene94096:CDS |
| ATGAGGCAGGAAAAGTTTAG+TGG | 0.534064 | 5.4:+46480223 | MS.gene94096:CDS |
| GATGTCCATCAGTGAGCTGA+TGG | 0.534460 | 5.4:+46478922 | MS.gene94096:CDS |
| AGAGCCGATCCGATGATAAA+CGG | 0.535121 | 5.4:+46478792 | MS.gene94096:CDS |
| AATCCAGAGGTTAGTGCAAG+TGG | 0.537993 | 5.4:+46480406 | MS.gene94096:intron |
| GAAGATGCAAGAGAGTGGTT+GGG | 0.539315 | 5.4:+46484269 | MS.gene94096:CDS |
| TGATCGAGTGGCATGGGTGG+AGG | 0.539487 | 5.4:+46478868 | MS.gene94096:CDS |
| GGGTGAATTATGGTAAAGGA+TGG | 0.539666 | 5.4:+46477802 | MS.gene94096:CDS |
| GAAAATGGGGAGTATGAGAT+GGG | 0.539759 | 5.4:+46482750 | MS.gene94096:CDS |
| TTCACAGCATCTTTGAAGAG+AGG | 0.539933 | 5.4:-46480770 | None:intergenic |
| GCAATCACCTCCTCCGTTAG+CGG | 0.541642 | 5.4:-46477685 | None:intergenic |
| GAGGTGGAGGAAGGAAGAGG+AGG | 0.544566 | 5.4:-46477630 | None:intergenic |
| GTTACGATTGATAATACGCA+TGG | 0.544599 | 5.4:-46477943 | None:intergenic |
| TCAATTCAACTTGATCCTGT+TGG | 0.546151 | 5.4:+46481788 | MS.gene94096:CDS |
| TGGCTACATTAATGGGAAAG+TGG | 0.548273 | 5.4:+46482253 | MS.gene94096:CDS |
| AGATTCGGAGCGAGATGAGG+TGG | 0.549375 | 5.4:-46477646 | None:intergenic |
| TCTCCCATCAGTTGAAGCAT+AGG | 0.549881 | 5.4:-46480995 | None:intergenic |
| AAGCCCGACAGCAAGGAAGT+TGG | 0.550357 | 5.4:+46484355 | MS.gene94096:CDS |
| TAATGGAGAATATGGTGGAA+AGG | 0.550804 | 5.4:+46482296 | MS.gene94096:CDS |
| GCTCGCGGTGGCTCATACGG+CGG | 0.551876 | 5.4:+46477722 | MS.gene94096:CDS |
| TTTCGGTGAAATTCACCTCA+AGG | 0.552799 | 5.4:+46478001 | MS.gene94096:CDS |
| GGTACCTACCGCTACGTAGG+AGG | 0.552879 | 5.4:+46484324 | MS.gene94096:CDS |
| AAACCGTTTAATCCATTACT+GGG | 0.553076 | 5.4:+46481024 | MS.gene94096:CDS |
| GATGGCTCCAGTGGACAATA+CGG | 0.554147 | 5.4:+46478940 | MS.gene94096:CDS |
| GATTGATCGCAATCCTCACC+AGG | 0.554267 | 5.4:+46482070 | MS.gene94096:CDS |
| TCGTCTAAAAGATGCTCAGA+AGG | 0.554380 | 5.4:-46484408 | None:intergenic |
| GCATGTCACTGTGAGGGTAC+AGG | 0.555198 | 5.4:+46481716 | MS.gene94096:CDS |
| ACATTAAATGAACTTACCCC+AGG | 0.557542 | 5.4:+46482417 | MS.gene94096:CDS |
| CTGCCTATGCTTCAACTGAT+GGG | 0.557550 | 5.4:+46480992 | MS.gene94096:CDS |
| TATGTTAATGGAGAATATGG+TGG | 0.561381 | 5.4:+46482291 | MS.gene94096:CDS |
| GAGCGAGATGAGGTGGAGGA+AGG | 0.563441 | 5.4:-46477639 | None:intergenic |
| GGATGATGATATAAAATCAC+GGG | 0.563992 | 5.4:-46480503 | None:intergenic |
| AGGATAGTGAGCAAATTATG+AGG | 0.566818 | 5.4:+46479026 | MS.gene94096:CDS |
| TGGAGCCATCAGCTCACTGA+TGG | 0.567673 | 5.4:-46478927 | None:intergenic |
| TCGTGATGATCGAGTGGCAT+GGG | 0.570755 | 5.4:+46478862 | MS.gene94096:CDS |
| ATGGAGAATATGGTGGAAAG+GGG | 0.573178 | 5.4:+46482298 | MS.gene94096:CDS |
| AAGCACAAATACTATGGAAG+CGG | 0.573964 | 5.4:+46482343 | MS.gene94096:CDS |
| TCCGTTAGCGGAGATTGGTA+CGG | 0.574083 | 5.4:-46477673 | None:intergenic |
| CAGGATCAAGTTGAATTGAT+CGG | 0.574504 | 5.4:-46481784 | None:intergenic |
| GCTACTTAAGCAGAAGCAGT+TGG | 0.575074 | 5.4:+46479081 | MS.gene94096:CDS |
| AATGGAAAACCGTTTATCAT+CGG | 0.575297 | 5.4:-46478801 | None:intergenic |
| CAGTGAGCTGATGGCTCCAG+TGG | 0.576173 | 5.4:+46478931 | MS.gene94096:CDS |
| CGGTCAAAGATATGTTTCCT+CGG | 0.576214 | 5.4:+46478900 | MS.gene94096:CDS |
| GAATATGGTGGAAAGGGGAA+AGG | 0.576376 | 5.4:+46482303 | MS.gene94096:CDS |
| TTATATCTAGTAGGAAACTT+GGG | 0.576474 | 5.4:-46482375 | None:intergenic |
| AGGCATCGGCTTATGGAAGA+AGG | 0.576568 | 5.4:+46478983 | MS.gene94096:CDS |
| TCCGTACCAATCTCCGCTAA+CGG | 0.578210 | 5.4:+46477672 | MS.gene94096:CDS |
| TATGGTACAATGCGCATCCA+GGG | 0.578329 | 5.4:+46482002 | MS.gene94096:CDS |
| GTAAAGGATGGAGGCATCGA+TGG | 0.579108 | 5.4:+46477814 | MS.gene94096:CDS |
| GTCAGGATCAACGACCTCGT+TGG | 0.580129 | 5.4:+46477750 | MS.gene94096:CDS |
| GAAACGGCCGTATTGTCCAC+TGG | 0.581171 | 5.4:-46478947 | None:intergenic |
| CCGTTTCAACTGAAAAGCTA+AGG | 0.584451 | 5.4:+46478963 | MS.gene94096:CDS |
| GGAGGAGGTGATTGCAGCCG+CGG | 0.584807 | 5.4:+46477693 | MS.gene94096:CDS |
| GCTAGAGCAGCGACAACGGC+AGG | 0.585865 | 5.4:+46482791 | MS.gene94096:CDS |
| ACTGCGGAGATGCAGCAGAA+AGG | 0.588268 | 5.4:-46477573 | None:intergenic |
| CAACTGAAAAGCTAAGGCAT+CGG | 0.590488 | 5.4:+46478969 | MS.gene94096:CDS |
| TTGAGGTGAATTTCACCGAA+AGG | 0.590833 | 5.4:-46477999 | None:intergenic |
| TGGATGATGATATAAAATCA+CGG | 0.592514 | 5.4:-46480504 | None:intergenic |
| CGATGGTTCGTGTTGCAAGA+TGG | 0.594774 | 5.4:+46477831 | MS.gene94096:CDS |
| TTAATCATTGTAGACAGAGA+AGG | 0.595043 | 5.4:+46480132 | MS.gene94096:intron |
| GGTTAGTGCAAGTGGGTCTG+AGG | 0.595559 | 5.4:+46480414 | MS.gene94096:CDS |
| GGAACCACGATGGTTTGCTA+GGG | 0.596524 | 5.4:+46484290 | MS.gene94096:CDS |
| GGATAGAAATGGGAAAACAT+TGG | 0.596727 | 5.4:+46482233 | MS.gene94096:CDS |
| GTAGACAGAGAAGGTTGACC+TGG | 0.597103 | 5.4:+46480141 | MS.gene94096:intron |
| TGAAATATTCCAGTGGAGCA+AGG | 0.598119 | 5.4:+46481838 | MS.gene94096:CDS |
| TGAATTATGGTAAAGGATGG+AGG | 0.598506 | 5.4:+46477805 | MS.gene94096:CDS |
| TACGGCTAGAGCAGCGACAA+CGG | 0.603059 | 5.4:+46482787 | MS.gene94096:CDS |
| ACATATAAAAGCACCTGGTG+AGG | 0.604072 | 5.4:-46482083 | None:intergenic |
| CTCGTGATGATCGAGTGGCA+TGG | 0.604319 | 5.4:+46478861 | MS.gene94096:CDS |
| CCGCGGAAGTCAGCTCGCGG+TGG | 0.607570 | 5.4:+46477710 | MS.gene94096:CDS |
| GGAAGATTCGGAGCGAGATG+AGG | 0.607636 | 5.4:-46477649 | None:intergenic |
| TTGATGAGAGCCAAAGACAA+TGG | 0.608013 | 5.4:+46480176 | MS.gene94096:CDS |
| GATTAAGGATAATATTGGGA+AGG | 0.610369 | 5.4:+46480702 | MS.gene94096:CDS |
| TCATCATGGAAGAAGCCTGT+TGG | 0.611229 | 5.4:-46480204 | None:intergenic |
| AGTGACATGCAACTATCATA+GGG | 0.611714 | 5.4:-46481703 | None:intergenic |
| AACAGGCTTCTTCCATGATG+AGG | 0.612253 | 5.4:+46480206 | MS.gene94096:CDS |
| CATCTTTGAAGAGAGGAGAG+AGG | 0.617333 | 5.4:-46480763 | None:intergenic |
| GGTCTTAACCTGGAATCTGT+AGG | 0.619295 | 5.4:-46482711 | None:intergenic |
| CAGCCGCGGAAGTCAGCTCG+CGG | 0.624216 | 5.4:+46477707 | MS.gene94096:CDS |
| GTCACTGTGAGGGTACAGGT+TGG | 0.625184 | 5.4:+46481720 | MS.gene94096:CDS |
| CAGATATTCCATTCCCAACG+AGG | 0.628322 | 5.4:-46477764 | None:intergenic |
| ATGAGCGGGAGATTGATGAT+CGG | 0.628453 | 5.4:-46477598 | None:intergenic |
| TGATGATCGAGTGGCATGGG+TGG | 0.629154 | 5.4:+46478865 | MS.gene94096:CDS |
| TATTGGGAAGCCCGACAGCA+AGG | 0.630226 | 5.4:+46484348 | MS.gene94096:CDS |
| GAGAGCCAAAGACAATGGAA+TGG | 0.630637 | 5.4:+46480181 | MS.gene94096:CDS |
| TCAGAGGTATTTGGAAAATG+GGG | 0.638243 | 5.4:+46482737 | MS.gene94096:CDS |
| ATCCAGAGGTTAGTGCAAGT+GGG | 0.638560 | 5.4:+46480407 | MS.gene94096:intron |
| AGCACAGACCTTTAATCCTG+GGG | 0.641750 | 5.4:-46482433 | None:intergenic |
| TGAGACTCGTGATGATCGAG+TGG | 0.643755 | 5.4:+46478856 | MS.gene94096:CDS |
| CTTAACCTGGAATCTGTAGG+TGG | 0.645465 | 5.4:-46482708 | None:intergenic |
| CTTGACCGAGCTTATGAATG+GGG | 0.646861 | 5.4:+46480814 | MS.gene94096:CDS |
| CCACCGCGAGCTGACTTCCG+CGG | 0.647447 | 5.4:-46477710 | None:intergenic |
| GATCTCTGTAGTTGGATCCG+AGG | 0.648525 | 5.4:+46480567 | MS.gene94096:CDS |
| ATATCCTCCTACGTAGCGGT+AGG | 0.649744 | 5.4:-46484328 | None:intergenic |
| GCTCGGAAGATGCAAGAGAG+TGG | 0.652263 | 5.4:+46484264 | MS.gene94096:CDS |
| ATGGGTGGAGGCTTTGTATG+CGG | 0.654208 | 5.4:+46478880 | MS.gene94096:CDS |
| AGGAGGCATGGAGAGATGAG+CGG | 0.654451 | 5.4:-46477613 | None:intergenic |
| CCCAATATCCTCCTACGTAG+CGG | 0.655345 | 5.4:-46484332 | None:intergenic |
| TATCTAGTAGGAAACTTGGG+AGG | 0.655367 | 5.4:-46482372 | None:intergenic |
| CTCTGTAGTTGGATCCGAGG+GGG | 0.656234 | 5.4:+46480570 | MS.gene94096:CDS |
| CCAGGTTAAGACCAGATCAG+AGG | 0.659521 | 5.4:+46482721 | MS.gene94096:CDS |
| AGAGTGGTTGGGAACCACGA+TGG | 0.662475 | 5.4:+46484280 | MS.gene94096:CDS |
| AAAACCGTTTATCATCGGAT+CGG | 0.669220 | 5.4:-46478796 | None:intergenic |
| GTTAGCGGAGATTGGTACGG+AGG | 0.678256 | 5.4:-46477670 | None:intergenic |
| AGCTCACTGATGGACATCCG+AGG | 0.683633 | 5.4:-46478917 | None:intergenic |
| AGATTGATGATCGGAGACTG+CGG | 0.689607 | 5.4:-46477589 | None:intergenic |
| GTACCAATCTCCGCTAACGG+AGG | 0.697880 | 5.4:+46477675 | MS.gene94096:CDS |
| GACATGCAACTATCATAGGG+TGG | 0.699648 | 5.4:-46481700 | None:intergenic |
| TCTCTGTAGTTGGATCCGAG+GGG | 0.707988 | 5.4:+46480569 | MS.gene94096:CDS |
| ATGATGGTGAAATATTCCAG+TGG | 0.713311 | 5.4:+46481831 | MS.gene94096:CDS |
| GAGGTTCATTAAAATAAACA+GGG | 0.715478 | 5.4:-46480744 | None:intergenic |
| GATAGTTGCATGTCACTGTG+AGG | 0.719382 | 5.4:+46481709 | MS.gene94096:CDS |
| ATAGTTGCATGTCACTGTGA+GGG | 0.729407 | 5.4:+46481710 | MS.gene94096:CDS |
| TTTGCTAGGGATAAAACAAG+CGG | 0.731198 | 5.4:+46484303 | MS.gene94096:CDS |
| AGCGGTACCTACCGCTACGT+AGG | 0.741484 | 5.4:+46484321 | MS.gene94096:CDS |
| TTATCCCTAGCAAACCATCG+TGG | 0.748499 | 5.4:-46484294 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 46477567 | 46484449 | 46477567 | ID=MS.gene94096 |
| chr5.4 | mRNA | 46477567 | 46484449 | 46477567 | ID=MS.gene94096.t1;Parent=MS.gene94096 |
| chr5.4 | exon | 46477567 | 46478022 | 46477567 | ID=MS.gene94096.t1.exon1;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46477567 | 46478022 | 46477567 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46478776 | 46479132 | 46478776 | ID=MS.gene94096.t1.exon2;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46478776 | 46479132 | 46478776 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46480145 | 46480244 | 46480145 | ID=MS.gene94096.t1.exon3;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46480145 | 46480244 | 46480145 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46480413 | 46480843 | 46480413 | ID=MS.gene94096.t1.exon4;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46480413 | 46480843 | 46480413 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46480946 | 46481101 | 46480946 | ID=MS.gene94096.t1.exon5;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46480946 | 46481101 | 46480946 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46481692 | 46481859 | 46481692 | ID=MS.gene94096.t1.exon6;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46481692 | 46481859 | 46481692 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46481951 | 46482091 | 46481951 | ID=MS.gene94096.t1.exon7;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46481951 | 46482091 | 46481951 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46482216 | 46482446 | 46482216 | ID=MS.gene94096.t1.exon8;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46482216 | 46482446 | 46482216 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46482699 | 46482812 | 46482699 | ID=MS.gene94096.t1.exon9;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46482699 | 46482812 | 46482699 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| chr5.4 | exon | 46484264 | 46484449 | 46484264 | ID=MS.gene94096.t1.exon10;Parent=MS.gene94096.t1 |
| chr5.4 | CDS | 46484264 | 46484449 | 46484264 | ID=cds.MS.gene94096.t1;Parent=MS.gene94096.t1 |
| Gene Sequence |
| Protein sequence |
