AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054798


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054798.t1 MTR_3g099570 85.714 168 24 0 1 168 17 184 4.62e-93 268
MS.gene054798.t1 MTR_3g099580 70.760 171 46 2 1 168 17 186 2.56e-63 193
MS.gene054798.t1 MTR_3g099680 60.000 205 40 4 1 164 17 220 2.99e-62 191
MS.gene054798.t1 MTR_3g099980 60.591 203 40 4 1 164 17 218 3.65e-62 191
MS.gene054798.t1 MTR_3g099540 61.818 110 42 0 1 110 18 127 2.38e-42 138
MS.gene054798.t1 MTR_8g007020 42.771 166 91 3 1 162 14 179 5.06e-31 110
MS.gene054798.t1 MTR_8g007035 53.271 107 47 2 1 104 14 120 5.16e-31 111
MS.gene054798.t1 MTR_1g090190 34.375 160 105 0 7 166 20 179 3.44e-30 108
MS.gene054798.t1 MTR_4g114870 45.690 116 58 2 8 118 27 142 9.44e-27 103
MS.gene054798.t1 MTR_4g114870 45.545 101 50 2 9 104 190 290 1.94e-22 92.4
MS.gene054798.t1 MTR_2g088990 41.176 136 71 3 6 132 21 156 2.84e-25 97.4
MS.gene054798.t1 MTR_6g083240 36.709 158 90 4 5 155 22 176 1.87e-22 88.6
MS.gene054798.t1 MTR_8g099220 43.689 103 54 2 7 105 26 128 3.09e-22 86.7
MS.gene054798.t1 MTR_5g006040 39.167 120 70 2 7 123 23 142 4.08e-22 87.8
MS.gene054798.t1 MTR_6g080660 41.414 99 58 0 10 108 28 126 7.21e-21 85.5
MS.gene054798.t1 MTR_7g086140 44.554 101 54 2 6 104 22 122 3.30e-20 82.4
MS.gene054798.t1 MTR_7g086190 44.554 101 54 2 6 104 22 122 3.30e-20 82.4
MS.gene054798.t1 MTR_2g043730 43.137 102 54 3 5 105 27 125 5.63e-20 80.9
MS.gene054798.t1 MTR_6g023760 40.196 102 60 1 4 104 20 121 1.05e-19 81.6
MS.gene054798.t1 MTR_0334s0010 40.777 103 58 1 5 104 23 125 1.67e-19 81.6
MS.gene054798.t1 MTR_1g112700 34.328 134 76 3 5 126 28 161 4.04e-19 80.1
MS.gene054798.t1 MTR_7g086160 43.000 100 55 2 7 104 24 123 8.64e-19 79.0
MS.gene054798.t1 MTR_7g086090 40.196 102 60 1 6 106 22 123 8.70e-19 80.5
MS.gene054798.t1 MTR_1g090420 33.088 136 83 4 7 138 25 156 9.41e-19 80.1
MS.gene054798.t1 MTR_1g105130 41.584 101 57 2 6 104 21 121 1.31e-18 78.2
MS.gene054798.t1 MTR_1g105120 41.584 101 57 2 6 104 21 121 1.31e-18 78.2
MS.gene054798.t1 MTR_4g067200 42.017 119 62 4 1 112 19 137 1.72e-18 78.2
MS.gene054798.t1 MTR_4g066110 42.017 119 62 4 1 112 19 137 1.72e-18 78.2
MS.gene054798.t1 MTR_2g025580 35.043 117 74 2 1 115 18 134 5.90e-18 76.6
MS.gene054798.t1 MTR_1g104800 38.614 101 59 3 7 104 22 122 1.88e-17 75.9
MS.gene054798.t1 MTR_8g463180 36.667 120 72 3 5 121 19 137 2.96e-17 77.4
MS.gene054798.t1 MTR_7g086220 40.659 91 53 1 6 95 21 111 3.00e-17 74.7
MS.gene054798.t1 MTR_7g086100 37.500 104 64 1 6 108 22 125 9.68e-17 74.7
MS.gene054798.t1 MTR_2g043790 42.424 99 53 3 5 102 2 97 2.73e-16 70.5
MS.gene054798.t1 MTR_7g086280 36.538 104 65 1 6 108 22 125 3.17e-16 72.4
MS.gene054798.t1 MTR_4g007250 39.423 104 61 2 4 106 23 125 3.36e-16 72.4
MS.gene054798.t1 MTR_7g086230 36.538 104 65 1 6 108 22 125 3.38e-16 72.4
MS.gene054798.t1 MTR_2g101300 37.600 125 67 4 7 122 24 146 3.45e-16 72.8
MS.gene054798.t1 MTR_4g081100 36.697 109 64 2 1 104 23 131 2.13e-14 68.6
MS.gene054798.t1 MTR_3g089035 40.206 97 54 3 7 102 30 123 8.64e-14 64.7
MS.gene054798.t1 MTR_2g083250 37.143 105 59 2 7 104 23 127 1.14e-13 67.0
MS.gene054798.t1 MTR_4g114830 36.170 94 53 3 13 99 32 125 1.24e-13 65.1
MS.gene054798.t1 MTR_4g130800 40.741 81 47 1 25 104 49 129 4.65e-13 65.5
MS.gene054798.t1 MTR_4g130800 40.741 81 47 1 25 104 48 128 5.63e-13 65.1
MS.gene054798.t1 MTR_4g077787 28.676 136 87 4 12 139 34 167 6.00e-13 63.9
MS.gene054798.t1 MTR_6g022170 35.922 103 60 2 7 104 31 132 7.09e-13 63.2
MS.gene054798.t1 MTR_6g013420 31.902 163 89 6 7 166 35 178 1.11e-12 63.2
MS.gene054798.t1 MTR_2g090580 31.944 144 83 4 12 142 29 170 2.00e-12 62.8
MS.gene054798.t1 MTR_4g130780 36.145 83 52 1 24 105 48 130 2.87e-12 62.0
MS.gene054798.t1 MTR_4g111640 29.104 134 87 4 12 138 34 166 6.81e-12 60.8
MS.gene054798.t1 MTR_2g090575 36.458 96 58 2 12 104 27 122 8.44e-12 62.4
MS.gene054798.t1 MTR_4g112310 29.310 116 80 2 24 138 2 116 1.67e-11 58.9
MS.gene054798.t1 MTR_8g088830 36.735 98 57 4 7 102 25 119 1.76e-11 58.5
MS.gene054798.t1 MTR_3g089005 35.922 103 62 3 1 102 24 123 2.85e-11 58.2
MS.gene054798.t1 MTR_8g089110 36.082 97 58 3 7 102 25 118 3.03e-11 58.2
MS.gene054798.t1 MTR_4g124280 33.010 103 64 2 7 104 22 124 3.16e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054798.t1 AT1G72230 48.966 145 63 2 18 157 31 169 9.66e-39 130
MS.gene054798.t1 AT1G22480 58.511 94 39 0 16 109 25 118 1.63e-30 109
MS.gene054798.t1 AT2G32300 43.966 116 64 1 5 119 21 136 2.24e-26 100
MS.gene054798.t1 AT2G32300 43.966 116 64 1 5 119 51 166 5.03e-26 100
MS.gene054798.t1 AT2G31050 35.849 159 85 3 7 148 26 184 6.73e-25 95.5
MS.gene054798.t1 AT5G07475 39.161 143 76 3 7 145 27 162 1.06e-24 95.1
MS.gene054798.t1 AT3G60270 46.000 100 54 0 5 104 21 120 1.33e-24 94.7
MS.gene054798.t1 AT2G26720 42.478 113 63 2 7 117 26 138 4.26e-24 93.6
MS.gene054798.t1 AT3G27200 41.000 100 58 1 6 104 22 121 1.12e-22 89.4
MS.gene054798.t1 AT2G44790 48.421 95 47 1 18 110 36 130 1.14e-21 87.4
MS.gene054798.t1 AT3G17675 39.252 107 64 1 1 106 23 129 2.46e-21 84.3
MS.gene054798.t1 AT5G20230 36.585 164 85 5 10 154 25 188 2.38e-18 78.6
MS.gene054798.t1 AT5G26330 38.614 101 59 2 7 104 21 121 3.38e-16 72.8
MS.gene054798.t1 AT5G53870 30.612 147 94 3 12 150 32 178 1.77e-14 70.1
MS.gene054798.t1 AT3G60280 39.316 117 71 0 6 122 20 136 1.99e-14 68.6
MS.gene054798.t1 AT5G53870 30.612 147 94 3 12 150 32 178 2.23e-14 70.1
MS.gene054798.t1 AT2G25060 35.849 106 62 3 18 117 40 145 1.30e-12 63.2
MS.gene054798.t1 AT4G28365 31.707 123 73 3 12 123 31 153 1.22e-11 60.8
MS.gene054798.t1 AT1G45063 35.455 110 63 3 12 115 151 258 2.93e-11 60.5
MS.gene054798.t1 AT1G45063 35.455 110 63 3 12 115 151 258 3.48e-11 60.8
MS.gene054798.t1 AT5G15350 33.036 112 71 3 7 115 26 136 4.00e-11 58.9
MS.gene054798.t1 AT3G18590 34.615 104 61 2 7 104 24 126 4.13e-11 58.9
MS.gene054798.t1 AT1G79800 33.333 117 64 3 12 115 36 151 9.23e-11 58.2

Find 44 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTATGGCATTGCCGACTA+TGG 0.299229 3.3:-81972446 None:intergenic
ATTCCTAGACATTGCACATT+TGG 0.319782 3.3:-81972072 MS.gene054798:CDS
CACATTGTTGGAGATGGTTT+AGG 0.350314 3.3:-81972415 MS.gene054798:CDS
TGGCATGAAACTCTCTGTTA+AGG 0.390798 3.3:-81972052 MS.gene054798:CDS
GATCTCTATCCGTCATATAT+TGG 0.416236 3.3:-81971860 MS.gene054798:CDS
GATGGTTTAGGTTGGACAAT+TGG 0.425937 3.3:-81972403 MS.gene054798:CDS
CTAGCCCATGTAGTGTAATC+AGG 0.453147 3.3:+81972379 None:intergenic
ACAGTGTTCAACTATGTAGC+TGG 0.464587 3.3:-81972225 MS.gene054798:intron
TATATGACGGATAGAGATCC+AGG 0.466675 3.3:+81971864 None:intergenic
TTCCAGCTGTGCATAATGTA+TGG 0.484126 3.3:+81972172 None:intergenic
ATGGCAACAACCCACATTGT+TGG 0.499388 3.3:-81972427 MS.gene054798:CDS
ATGCCAAATGTGCAATGTCT+AGG 0.500016 3.3:+81972069 None:intergenic
GACCATACATTATGCACAGC+TGG 0.503333 3.3:-81972174 MS.gene054798:CDS
TTGGTCCTGATTACACTACA+TGG 0.504799 3.3:-81972384 MS.gene054798:CDS
ATCGGAAGGTGAACCTTTCG+TGG 0.505016 3.3:+81971987 None:intergenic
GCAGAAGGAGCGGCAGAAGA+AGG 0.505108 3.3:+81972024 None:intergenic
GGTGGTGGTTGTTGCGGCAG+CGG 0.517819 3.3:+81971960 None:intergenic
AACAATTGGTGAGATATTAG+TGG 0.520976 3.3:+81971906 None:intergenic
TGTTGCGGCAGCGGTGCGAT+CGG 0.526820 3.3:+81971969 None:intergenic
GCGGCAGCGGTGCGATCGGA+AGG 0.527471 3.3:+81971973 None:intergenic
ACAACCCACATTGTTGGAGA+TGG 0.532220 3.3:-81972421 MS.gene054798:CDS
GAAGGTGAACCTTTCGTGGA+TGG 0.547718 3.3:+81971991 None:intergenic
TTTGCAGTTGGTGACAGTCT+CGG 0.554211 3.3:-81972346 MS.gene054798:intron
TGTGGTAGGAGTAGTGGTGG+TGG 0.559960 3.3:+81971945 None:intergenic
ATGTGGGTTGTTGCCATAGT+CGG 0.565131 3.3:+81972433 None:intergenic
GAAGATGCACTTTGTGTGGT+AGG 0.585213 3.3:+81971931 None:intergenic
TTGTTGGAGATGGTTTAGGT+TGG 0.586690 3.3:-81972411 MS.gene054798:CDS
AGTGACAAAACCTTTGCAGT+TGG 0.590211 3.3:-81972358 MS.gene054798:CDS
GAACCTTTCGTGGATGGTGA+TGG 0.595492 3.3:+81971997 None:intergenic
AGTAGTGGTGGTGGTTGTTG+CGG 0.603984 3.3:+81971954 None:intergenic
CAGTGTTCAACTATGTAGCT+GGG 0.604419 3.3:-81972224 MS.gene054798:CDS
GATGGTGATGGTGTTGCAGA+AGG 0.618024 3.3:+81972009 None:intergenic
ACTTTGTGTGGTAGGAGTAG+TGG 0.618884 3.3:+81971939 None:intergenic
GAGACTGTCACCAACTGCAA+AGG 0.629029 3.3:+81972348 None:intergenic
TGGTCCTGATTACACTACAT+GGG 0.630389 3.3:-81972383 MS.gene054798:CDS
TAAACCATCTCCAACAATGT+GGG 0.636144 3.3:+81972417 None:intergenic
ACACCATCACCATCCACGAA+AGG 0.636152 3.3:-81972000 MS.gene054798:CDS
GGCTGAAGATGCACTTTGTG+TGG 0.645311 3.3:+81971927 None:intergenic
TATCTCACACCAATATATGA+CGG 0.646155 3.3:+81971851 None:intergenic
TTGTGTGGTAGGAGTAGTGG+TGG 0.649594 3.3:+81971942 None:intergenic
TCAATTAATACAGACAGCAG+TGG 0.671855 3.3:-81972147 MS.gene054798:CDS
CTAAACCATCTCCAACAATG+TGG 0.675206 3.3:+81972416 None:intergenic
TGATGGTGTTGCAGAAGGAG+CGG 0.712627 3.3:+81972014 None:intergenic
CTATGTAGCTGGGCACACAG+TGG 0.727318 3.3:-81972214 MS.gene054798:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACAATTGGTGAGATATTAG+TGG + chr3.3:81972394-81972413 None:intergenic 30.0%
GTGAAAAAGAAAGCAACAAT+TGG + chr3.3:81972408-81972427 None:intergenic 30.0%
!!! AATGTGTTCCTGCTTTTTTT+AGG + chr3.3:81972191-81972210 None:intergenic 30.0%
!!! ATGTGTTCCTGCTTTTTTTA+GGG + chr3.3:81972190-81972209 None:intergenic 30.0%
ATTCCTAGACATTGCACATT+TGG - chr3.3:81972225-81972244 MS.gene054798:intron 35.0%
GATCTCTATCCGTCATATAT+TGG - chr3.3:81972437-81972456 MS.gene054798:CDS 35.0%
TAAACCATCTCCAACAATGT+GGG + chr3.3:81971883-81971902 None:intergenic 35.0%
TCAATTAATACAGACAGCAG+TGG - chr3.3:81972150-81972169 MS.gene054798:CDS 35.0%
!!! TGTGTTCCTGCTTTTTTTAG+GGG + chr3.3:81972189-81972208 None:intergenic 35.0%
!!! TTTTTTTAGGGGAATGGTTG+TGG + chr3.3:81972178-81972197 None:intergenic 35.0%
ACAGTGTTCAACTATGTAGC+TGG - chr3.3:81972072-81972091 MS.gene054798:CDS 40.0%
ACCATTCCCCTAAAAAAAGC+AGG - chr3.3:81972180-81972199 MS.gene054798:CDS 40.0%
AGTGACAAAACCTTTGCAGT+TGG - chr3.3:81971939-81971958 MS.gene054798:CDS 40.0%
ATGCCAAATGTGCAATGTCT+AGG + chr3.3:81972231-81972250 None:intergenic 40.0%
CACATTGTTGGAGATGGTTT+AGG - chr3.3:81971882-81971901 MS.gene054798:CDS 40.0%
CAGTGTTCAACTATGTAGCT+GGG - chr3.3:81972073-81972092 MS.gene054798:CDS 40.0%
CTAAACCATCTCCAACAATG+TGG + chr3.3:81971884-81971903 None:intergenic 40.0%
TGGCATGAAACTCTCTGTTA+AGG - chr3.3:81972245-81972264 MS.gene054798:intron 40.0%
TGGTCCTGATTACACTACAT+GGG - chr3.3:81971914-81971933 MS.gene054798:CDS 40.0%
TTCCAGCTGTGCATAATGTA+TGG + chr3.3:81972128-81972147 None:intergenic 40.0%
TTGGTCCTGATTACACTACA+TGG - chr3.3:81971913-81971932 MS.gene054798:CDS 40.0%
TTGTTGGAGATGGTTTAGGT+TGG - chr3.3:81971886-81971905 MS.gene054798:CDS 40.0%
!! CTTTCTTTTTCACTGCTTCC+TGG - chr3.3:81972415-81972434 MS.gene054798:CDS 40.0%
!! GATGGTTTAGGTTGGACAAT+TGG - chr3.3:81971894-81971913 MS.gene054798:CDS 40.0%
!! TATATGACGGATAGAGATCC+AGG + chr3.3:81972436-81972455 None:intergenic 40.0%
!!! TCCTGCTTTTTTTAGGGGAA+TGG + chr3.3:81972184-81972203 None:intergenic 40.0%
ACAACCCACATTGTTGGAGA+TGG - chr3.3:81971876-81971895 MS.gene054798:CDS 45.0%
ACTTTGTGTGGTAGGAGTAG+TGG + chr3.3:81972361-81972380 None:intergenic 45.0%
ATGGCAACAACCCACATTGT+TGG - chr3.3:81971870-81971889 MS.gene054798:CDS 45.0%
CTAGCCCATGTAGTGTAATC+AGG + chr3.3:81971921-81971940 None:intergenic 45.0%
GAAGATGCACTTTGTGTGGT+AGG + chr3.3:81972369-81972388 None:intergenic 45.0%
GACCATACATTATGCACAGC+TGG - chr3.3:81972123-81972142 MS.gene054798:CDS 45.0%
! ATGTGGGTTGTTGCCATAGT+CGG + chr3.3:81971867-81971886 None:intergenic 45.0%
!! TTTGCAGTTGGTGACAGTCT+CGG - chr3.3:81971951-81971970 MS.gene054798:CDS 45.0%
ACACCATCACCATCCACGAA+AGG - chr3.3:81972297-81972316 MS.gene054798:intron 50.0%
ATCGGAAGGTGAACCTTTCG+TGG + chr3.3:81972313-81972332 None:intergenic 50.0%
GAACCTTTCGTGGATGGTGA+TGG + chr3.3:81972303-81972322 None:intergenic 50.0%
GAAGGTGAACCTTTCGTGGA+TGG + chr3.3:81972309-81972328 None:intergenic 50.0%
GAGACTGTCACCAACTGCAA+AGG + chr3.3:81971952-81971971 None:intergenic 50.0%
GGCTGAAGATGCACTTTGTG+TGG + chr3.3:81972373-81972392 None:intergenic 50.0%
TTGTGTGGTAGGAGTAGTGG+TGG + chr3.3:81972358-81972377 None:intergenic 50.0%
!! AGTAGTGGTGGTGGTTGTTG+CGG + chr3.3:81972346-81972365 None:intergenic 50.0%
!! GATGGTGATGGTGTTGCAGA+AGG + chr3.3:81972291-81972310 None:intergenic 50.0%
!! TGATGGTGTTGCAGAAGGAG+CGG + chr3.3:81972286-81972305 None:intergenic 50.0%
CTATGTAGCTGGGCACACAG+TGG - chr3.3:81972083-81972102 MS.gene054798:CDS 55.0%
TGTGGTAGGAGTAGTGGTGG+TGG + chr3.3:81972355-81972374 None:intergenic 55.0%
GCAGAAGGAGCGGCAGAAGA+AGG + chr3.3:81972276-81972295 None:intergenic 60.0%
TGTTGCGGCAGCGGTGCGAT+CGG + chr3.3:81972331-81972350 None:intergenic 65.0%
! GGTGGTGGTTGTTGCGGCAG+CGG + chr3.3:81972340-81972359 None:intergenic 65.0%
GCGGCAGCGGTGCGATCGGA+AGG + chr3.3:81972327-81972346 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 81971855 81972464 81971855 ID=MS.gene054798
chr3.3 mRNA 81971855 81972464 81971855 ID=MS.gene054798.t1;Parent=MS.gene054798
chr3.3 exon 81972347 81972464 81972347 ID=MS.gene054798.t1.exon1;Parent=MS.gene054798.t1
chr3.3 CDS 81972347 81972464 81972347 ID=cds.MS.gene054798.t1;Parent=MS.gene054798.t1
chr3.3 exon 81971855 81972243 81971855 ID=MS.gene054798.t1.exon2;Parent=MS.gene054798.t1
chr3.3 CDS 81971855 81972243 81971855 ID=cds.MS.gene054798.t1;Parent=MS.gene054798.t1
Gene Sequence

>MS.gene054798

ATGGCATTGCCGACTATGGCAACAACCCACATTGTTGGAGATGGTTTAGGTTGGACAATTGGTCCTGATTACACTACATGGGCTAGTGACAAAACCTTTGCAGTTGGTGACAGTCTCGGTAAGTATAATAATTTAACGAACTTTTATCAATTTCATTTTTTTTTAGTTTTAAATTGCAATCACAATTTACGAAACTAATGTATGTAACTTTACTATGACAGTGTTCAACTATGTAGCTGGGCACACAGTGGATGAAGTTAAAGAAAGTGACCATACATTATGCACAGCTGGAAATTCAATTAATACAGACAGCAGTGGTGCCACAACCATTCCCCTAAAAAAAGCAGGAACACATTACTTCATATGTGCTATTCCTAGACATTGCACATTTGGCATGAAACTCTCTGTTAAGGTTAATCCTTCTTCTGCCGCTCCTTCTGCAACACCATCACCATCCACGAAAGGTTCACCTTCCGATCGCACCGCTGCCGCAACAACCACCACCACTACTCCTACCACACAAAGTGCATCTTCAGCCACTAATATCTCACCAATTGTTGCTTTCTTTTTCACTGCTTCCTGGATCTCTATCCGTCATATATTGGTGTGA

Protein sequence

>MS.gene054798.t1

MALPTMATTHIVGDGLGWTIGPDYTTWASDKTFAVGDSLVFNYVAGHTVDEVKESDHTLCTAGNSINTDSSGATTIPLKKAGTHYFICAIPRHCTFGMKLSVKVNPSSAAPSATPSPSTKGSPSDRTAAATTTTTTPTTQSASSATNISPIVAFFFTASWISIRHILV