Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055316.t1 | XP_024632434.1 | 98.9 | 90 | 1 | 0 | 84 | 173 | 387 | 476 | 8.40E-44 | 186.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055316.t1 | D2XNQ9 | 90.2 | 92 | 9 | 0 | 82 | 173 | 389 | 480 | 3.2e-44 | 179.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055316.t1 | A0A072VR23 | 98.9 | 87 | 1 | 0 | 87 | 173 | 387 | 473 | 5.7e-42 | 180.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055316 | MS.gene32110 | 0.826889 | 2.12E-54 | -1.69E-46 |
| MS.gene055316 | MS.gene40041 | 0.808392 | 3.17E-50 | -1.69E-46 |
| MS.gene055316 | MS.gene40045 | 0.814127 | 1.80E-51 | -1.69E-46 |
| MS.gene055316 | MS.gene55257 | 0.806613 | 7.55E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055316.t1 | MTR_1g071130 | 95.266 | 169 | 5 | 1 | 5 | 173 | 284 | 449 | 4.88e-113 | 328 |
| MS.gene055316.t1 | MTR_1g115145 | 94.611 | 167 | 9 | 0 | 7 | 173 | 307 | 473 | 6.11e-112 | 327 |
| MS.gene055316.t1 | MTR_1g073400 | 94.083 | 169 | 6 | 1 | 5 | 173 | 310 | 474 | 2.05e-110 | 323 |
| MS.gene055316.t1 | MTR_3g106480 | 89.941 | 169 | 17 | 0 | 5 | 173 | 310 | 478 | 1.39e-108 | 318 |
| MS.gene055316.t1 | MTR_3g106420 | 89.349 | 169 | 18 | 0 | 5 | 173 | 312 | 480 | 1.63e-107 | 315 |
| MS.gene055316.t1 | MTR_3g106470 | 89.286 | 168 | 18 | 0 | 5 | 172 | 285 | 452 | 2.68e-105 | 309 |
| MS.gene055316.t1 | MTR_3g106485 | 89.349 | 169 | 14 | 2 | 5 | 173 | 310 | 474 | 2.60e-104 | 307 |
| MS.gene055316.t1 | MTR_3g106430 | 88.095 | 168 | 16 | 2 | 5 | 172 | 310 | 473 | 3.80e-101 | 299 |
| MS.gene055316.t1 | MTR_1g083190 | 85.207 | 169 | 17 | 1 | 5 | 173 | 312 | 472 | 1.02e-98 | 293 |
| MS.gene055316.t1 | MTR_8g063830 | 85.897 | 156 | 21 | 1 | 4 | 159 | 271 | 425 | 8.77e-91 | 271 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055316.t1 | AT5G25260 | 62.424 | 165 | 50 | 2 | 5 | 169 | 310 | 462 | 2.09e-67 | 212 |
| MS.gene055316.t1 | AT5G25250 | 60.366 | 164 | 51 | 2 | 5 | 168 | 310 | 459 | 3.55e-60 | 194 |
| MS.gene055316.t1 | AT5G64870 | 56.098 | 164 | 61 | 1 | 5 | 168 | 311 | 463 | 7.11e-55 | 180 |
Find 0 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGTTTATTTTTTAAGTAC+TGG | - | 11199:19238-19257 | MS.gene055316:CDS | 15.0% |
| ! | AAGATTAGTATTTGGACTAA+TGG | - | 11199:19612-19631 | MS.gene055316:CDS | 25.0% |
| ! | TTAGTCCAAATACTAATCTT+AGG | + | 11199:19612-19631 | MS.gene055316:intergenic | 25.0% |
| ! | TTGAACCTAAGATTAGTATT+TGG | - | 11199:19604-19623 | MS.gene055316:CDS | 25.0% |
| !!! | TTAAGTACTGGTTGAAATTT+TGG | - | 11199:19250-19269 | MS.gene055316:CDS | 25.0% |
| ATTAGTATTTGGACTAATGG+AGG | - | 11199:19615-19634 | MS.gene055316:CDS | 30.0% | |
| ! | AGGGATTACTTGATGATAAA+TGG | - | 11199:19531-19550 | MS.gene055316:CDS | 30.0% |
| ! | GATTACTTGATGATAAATGG+TGG | - | 11199:19534-19553 | MS.gene055316:CDS | 30.0% |
| ! | GTTGAAATTTTGGATGGTTT+AGG | - | 11199:19260-19279 | MS.gene055316:CDS | 30.0% |
| AATACACTTCTCAATGCACT+TGG | - | 11199:19489-19508 | MS.gene055316:CDS | 35.0% | |
| AGCAATTCTGTATGTGAAGA+AGG | - | 11199:19329-19348 | MS.gene055316:CDS | 35.0% | |
| CAAAGAAAAAAGAGGCAGAA+GGG | - | 11199:19430-19449 | MS.gene055316:CDS | 35.0% | |
| GCTATACGCAAAGAAAAAAG+AGG | - | 11199:19422-19441 | MS.gene055316:CDS | 35.0% | |
| ! | TTAAGAGCTTTTGTCGGATA+AGG | + | 11199:19772-19791 | MS.gene055316:intergenic | 35.0% |
| !!! | GTACTGGTTGAAATTTTGGA+TGG | - | 11199:19254-19273 | MS.gene055316:CDS | 35.0% |
| GCAAAGAAAAAAGAGGCAGA+AGG | - | 11199:19429-19448 | MS.gene055316:CDS | 40.0% | |
| TCATGGACAGTCTTAAACAG+AGG | + | 11199:19714-19733 | MS.gene055316:intergenic | 40.0% | |
| TCTGTATGTGAAGAAGGTAG+AGG | - | 11199:19335-19354 | MS.gene055316:CDS | 40.0% | |
| TTAGGTACAAGAGGCTAACT+GGG | - | 11199:19278-19297 | MS.gene055316:CDS | 40.0% | |
| TTGGATGGTTTAGGTACAAG+AGG | - | 11199:19269-19288 | MS.gene055316:CDS | 40.0% | |
| TTTAGGTACAAGAGGCTAAC+TGG | - | 11199:19277-19296 | MS.gene055316:CDS | 40.0% | |
| ! | AAACGACTACACAGCTCTAA+GGG | - | 11199:19512-19531 | MS.gene055316:CDS | 40.0% |
| ! | GGAATAACGGAAGGTTCTAT+GGG | - | 11199:19651-19670 | MS.gene055316:CDS | 40.0% |
| AAGACTGTCCATGAGCAAAC+AGG | - | 11199:19720-19739 | MS.gene055316:intron | 45.0% | |
| ACTAATGGAGGTGACAATGG+AGG | - | 11199:19627-19646 | MS.gene055316:CDS | 45.0% | |
| AGGTTCTATGGGCATGAAAG+AGG | - | 11199:19662-19681 | MS.gene055316:CDS | 45.0% | |
| CAGAAGGGATTGTGACACTT+GGG | - | 11199:19445-19464 | MS.gene055316:CDS | 45.0% | |
| GAAACGACTACACAGCTCTA+AGG | - | 11199:19511-19530 | MS.gene055316:CDS | 45.0% | |
| GATGGCTAAGATCAATGCTG+AGG | - | 11199:19569-19588 | MS.gene055316:CDS | 45.0% | |
| TGACAATGGAGGCGGAATAA+CGG | - | 11199:19638-19657 | MS.gene055316:CDS | 45.0% | |
| TGGACAGTCTTAAACAGAGG+TGG | + | 11199:19711-19730 | MS.gene055316:intergenic | 45.0% | |
| TGGACTAATGGAGGTGACAA+TGG | - | 11199:19624-19643 | MS.gene055316:CDS | 45.0% | |
| TGGTGGCATGTTTCAAGAGA+TGG | - | 11199:19551-19570 | MS.gene055316:CDS | 45.0% | |
| ! | CGGAATAACGGAAGGTTCTA+TGG | - | 11199:19650-19669 | MS.gene055316:CDS | 45.0% |
| AATGGAGGTGACAATGGAGG+CGG | - | 11199:19630-19649 | MS.gene055316:CDS | 50.0% | |
| ATCAATGCTGAGGCAGTACG+TGG | - | 11199:19579-19598 | MS.gene055316:CDS | 50.0% | |
| ATGGGCATGAAAGAGGTTGC+TGG | - | 11199:19669-19688 | MS.gene055316:CDS | 50.0% | |
| ATGTTGCCTCCTGCTTGGAT+GGG | - | 11199:19744-19763 | MS.gene055316:intron | 50.0% | |
| GATAAGGTTCCCATCCAAGC+AGG | + | 11199:19756-19775 | MS.gene055316:intergenic | 50.0% | |
| GCAACATGCCTGTTTGCTCA+TGG | + | 11199:19731-19750 | MS.gene055316:intergenic | 50.0% | |
| GCAGAAGGGATTGTGACACT+TGG | - | 11199:19444-19463 | MS.gene055316:CDS | 50.0% | |
| GTGACACTTGGGAATGCTCA+AGG | - | 11199:19456-19475 | MS.gene055316:CDS | 50.0% | |
| ! | AATGGAGGCGGAATAACGGA+AGG | - | 11199:19642-19661 | MS.gene055316:CDS | 50.0% |
| AAGGTTCCCATCCAAGCAGG+AGG | + | 11199:19753-19772 | MS.gene055316:intergenic | 55.0% | |
| CATGTTGCCTCCTGCTTGGA+TGG | - | 11199:19743-19762 | MS.gene055316:intron | 55.0% | |
| CAGGCATGTTGCCTCCTGCT+TGG | - | 11199:19739-19758 | MS.gene055316:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11199 | gene | 19229 | 19791 | 19229 | ID=MS.gene055316 |
| 11199 | mRNA | 19229 | 19791 | 19229 | ID=MS.gene055316.t1;Parent=MS.gene055316 |
| 11199 | exon | 19780 | 19791 | 19780 | ID=MS.gene055316.t1.exon1;Parent=MS.gene055316.t1 |
| 11199 | CDS | 19780 | 19791 | 19780 | ID=cds.MS.gene055316.t1;Parent=MS.gene055316.t1 |
| 11199 | exon | 19229 | 19738 | 19229 | ID=MS.gene055316.t1.exon2;Parent=MS.gene055316.t1 |
| 11199 | CDS | 19229 | 19738 | 19229 | ID=cds.MS.gene055316.t1;Parent=MS.gene055316.t1 |
| Gene Sequence |
| Protein sequence |
