AlfalfaGEDB | MS.gene055622

M. sativa cultivar XinJiangDaYe / MS.gene055622

KEGG: K07904
GO: GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00071.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055622.t1	Q40193.1	100	55	0	0	1	55	1	55	1.30E-22	114.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055622.t1	Q40193	100.0	55	0	0	1	55	1	55	3.1e-25	114.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055622.t1	A0A445A9Q0	100.0	55	0	0	1	55	1	55	9.3e-23	114.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055622	MS.gene20629	0.807003	6.25E-50	-1.69E-46
MS.gene055622	MS.gene41117	0.850502	1.63E-60	-1.69E-46
MS.gene055622	MS.gene54678	0.820569	6.40E-53	-1.69E-46
MS.gene055622	MS.gene58509	0.804554	2.04E-49	-1.69E-46
MS.gene055622	MS.gene91292	0.807708	4.43E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055622.t1	MTR_4g064897	100.000	55	0	0	1	55	1	55	2.96e-35	117
MS.gene055622.t1	MTR_3g069510	92.727	55	4	0	1	55	1	55	1.29e-32	111
MS.gene055622.t1	MTR_2g005510	92.000	50	4	0	6	55	9	58	1.35e-28	100
MS.gene055622.t1	MTR_2g014990	79.630	54	11	0	2	55	3	56	1.54e-26	95.5
MS.gene055622.t1	MTR_2g075950	83.019	53	9	0	3	55	4	56	1.60e-26	95.5
MS.gene055622.t1	MTR_4g088090	79.630	54	11	0	2	55	3	56	2.20e-26	95.1
MS.gene055622.t1	MTR_4g079350	79.630	54	11	0	2	55	3	56	2.73e-26	95.1
MS.gene055622.t1	MTR_2g083860	84.615	52	8	0	3	54	4	55	2.95e-26	94.7
MS.gene055622.t1	MTR_2g090865	86.275	51	7	0	5	55	4	54	6.19e-26	94.0
MS.gene055622.t1	MTR_8g089985	73.469	49	13	0	6	54	11	59	1.10e-19	78.2
MS.gene055622.t1	MTR_8g027220	63.462	52	19	0	3	54	17	68	2.24e-19	77.4
MS.gene055622.t1	MTR_7g117910	64.151	53	19	0	2	54	10	62	3.23e-19	76.6
MS.gene055622.t1	MTR_6g079550	63.636	55	20	0	1	55	1	55	8.72e-19	75.5
MS.gene055622.t1	MTR_8g107070	72.000	50	14	0	6	55	8	57	9.65e-19	76.3
MS.gene055622.t1	MTR_8g036220	68.627	51	15	1	5	54	8	58	1.29e-18	75.5
MS.gene055622.t1	MTR_3g086980	67.347	49	16	0	6	54	10	58	1.40e-18	75.1
MS.gene055622.t1	MTR_1g078200	71.739	46	13	0	10	55	12	57	2.68e-17	71.6
MS.gene055622.t1	MTR_7g405790	71.111	45	13	0	11	55	14	58	3.50e-17	71.2
MS.gene055622.t1	MTR_3g053480	60.784	51	19	1	5	54	8	58	1.24e-16	66.2
MS.gene055622.t1	MTR_0262s0050	68.889	45	14	0	10	54	13	57	1.27e-16	70.1
MS.gene055622.t1	MTR_1g052475	61.224	49	19	0	6	54	9	57	1.28e-16	70.1
MS.gene055622.t1	MTR_7g051940	73.333	45	12	0	11	55	12	56	2.00e-16	69.3
MS.gene055622.t1	MTR_8g099250	63.265	49	17	1	5	52	8	56	7.47e-16	64.3
MS.gene055622.t1	MTR_8g006970	54.902	51	23	0	5	55	1	51	1.08e-12	58.9
MS.gene055622.t1	MTR_7g081700	58.333	48	20	0	8	55	4	51	1.34e-12	58.9
MS.gene055622.t1	MTR_1g068730	50.000	52	26	0	4	55	7	58	1.14e-11	56.6
MS.gene055622.t1	MTR_1g068740	50.000	52	26	0	4	55	7	58	1.17e-11	56.6
MS.gene055622.t1	MTR_1g068730	50.000	52	26	0	4	55	7	58	1.51e-11	55.8
MS.gene055622.t1	MTR_1g068740	50.000	52	26	0	4	55	7	58	2.52e-11	55.5
MS.gene055622.t1	MTR_1g115325	52.083	48	23	0	8	55	11	58	5.22e-11	55.1
MS.gene055622.t1	MTR_2g005500	53.061	49	23	0	7	55	4	52	5.74e-11	54.3
MS.gene055622.t1	MTR_7g100020	52.083	48	23	0	8	55	11	58	8.14e-11	54.3
MS.gene055622.t1	MTR_4g063160	52.083	48	23	0	8	55	11	58	8.22e-11	54.3
MS.gene055622.t1	MTR_8g006970	56.522	46	20	0	10	55	20	65	9.99e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055622.t1	AT1G07410	96.364	55	2	1	55	1	55	5.78e-34	114
MS.gene055622.t1	AT3G46830	94.545	55	3	1	55	1	55	2.37e-32	110
MS.gene055622.t1	AT3G46830	94.545	55	3	1	55	1	55	2.37e-32	110
MS.gene055622.t1	AT5G59150	92.727	55	4	1	55	1	55	2.98e-32	110
MS.gene055622.t1	AT1G09630	90.909	55	5	1	55	1	55	9.79e-32	108
MS.gene055622.t1	AT5G60860	83.333	54	9	2	55	3	56	2.65e-27	97.4
MS.gene055622.t1	AT5G45750	83.019	53	9	3	55	4	56	9.04e-27	96.3
MS.gene055622.t1	AT4G18800	83.019	53	9	3	55	4	56	1.25e-26	95.9
MS.gene055622.t1	AT1G16920	81.481	54	10	2	55	3	56	1.84e-26	95.5
MS.gene055622.t1	AT4G18430	79.630	54	11	2	55	3	56	5.12e-26	94.4
MS.gene055622.t1	AT3G15060	77.778	54	12	2	55	3	56	1.13e-25	93.6
MS.gene055622.t1	AT1G06400	79.630	54	11	2	55	3	56	1.38e-25	93.2
MS.gene055622.t1	AT1G28550	70.370	54	16	2	55	3	56	4.03e-23	87.0
MS.gene055622.t1	AT2G33870	69.811	53	16	3	55	4	56	1.80e-22	85.1
MS.gene055622.t1	AT5G47960	68.000	50	16	6	55	9	58	1.32e-19	77.8
MS.gene055622.t1	AT1G01200	73.333	45	12	10	54	26	70	1.39e-18	75.5
MS.gene055622.t1	AT1G18200	70.000	50	15	6	55	7	56	4.37e-18	73.9
MS.gene055622.t1	AT5G65270	73.333	45	12	10	54	15	59	6.94e-18	73.6
MS.gene055622.t1	AT3G12160	63.265	49	18	6	54	9	57	1.47e-17	72.4
MS.gene055622.t1	AT5G47520	69.388	49	15	6	54	8	56	1.97e-17	72.0
MS.gene055622.t1	AT4G39990	71.111	45	13	10	54	15	59	2.06e-17	72.0
MS.gene055622.t1	AT1G05810	67.391	46	15	10	55	10	55	5.84e-17	70.9
MS.gene055622.t1	AT3G07410	61.818	55	21	1	55	1	55	7.03e-17	70.5
MS.gene055622.t1	AT1G05810	67.391	46	15	10	55	56	101	9.87e-17	70.9
MS.gene055622.t1	AT1G73640	67.347	49	16	6	54	7	55	1.24e-16	70.1
MS.gene055622.t1	AT2G31680	67.391	46	15	10	55	10	55	2.11e-16	69.3
MS.gene055622.t1	AT2G43130	66.667	45	15	10	54	10	54	6.73e-16	68.2
MS.gene055622.t1	AT1G02130	58.333	48	20	8	55	4	51	3.64e-13	60.5
MS.gene055622.t1	AT5G47200	58.333	48	20	8	55	4	51	9.63e-13	59.3
MS.gene055622.t1	AT4G17530	58.333	48	20	8	55	4	51	1.06e-12	59.3
MS.gene055622.t1	AT3G11730	52.941	51	24	5	55	1	51	1.08e-11	56.6
MS.gene055622.t1	AT5G59840	52.083	48	23	8	55	11	58	8.26e-11	54.7
MS.gene055622.t1	AT3G53610	52.083	48	23	8	55	11	58	8.35e-11	54.7
MS.gene055622.t1	AT3G53610	52.083	48	23	8	55	11	58	8.35e-11	54.7
MS.gene055622.t1	AT3G53610	52.083	48	23	8	55	11	58	8.35e-11	54.7
MS.gene055622.t1	AT3G46060	52.083	48	23	8	55	11	58	8.43e-11	54.3
MS.gene055622.t1	AT3G46060	52.083	48	23	8	55	11	58	8.43e-11	54.3
MS.gene055622.t1	AT3G46060	52.083	48	23	8	55	11	58	8.43e-11	54.3

Find 11 sgRNAs with CRISPR-Local

Find 7 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGAAGTACCTGAAGGGTTC+TGG	0.372210	4.4:-41442476	None:intergenic
TTGGAGTCTAAATCTACTAT+TGG	0.376344	4.4:+41442439	MS.gene055622:CDS
ATTCGCCACCAGAACCCTTC+AGG	0.400128	4.4:+41442468	MS.gene055622:CDS
GTAAATCCAACATTCTCTCT+AGG	0.462112	4.4:+41442395	MS.gene055622:CDS
TTGATCGGAGATTCTGGTGT+TGG	0.486858	4.4:+41442373	MS.gene055622:CDS
GAGAAGATCTATGGCGCATA+GGG	0.496739	4.4:+41442315	None:intergenic
TGTAAACCTAGAGAGAATGT+TGG	0.541295	4.4:-41442401	None:intergenic
AAGATCTATGGCGCATAGGG+TGG	0.554795	4.4:+41442318	None:intergenic
GGAGAAGATCTATGGCGCAT+AGG	0.558633	4.4:+41442314	None:intergenic
AGAAATTGGAAGTACCTGAA+GGG	0.628923	4.4:-41442482	None:intergenic
AAGTACCTGAAGGGTTCTGG+TGG	0.681997	4.4:-41442473	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TACAAGAAATGAGTTTTGTT+TGG	+	chr4.4:41442420-41442439	MS.gene055622:CDS	25.0%
	TTGGAGTCTAAATCTACTAT+TGG	+	chr4.4:41442439-41442458	MS.gene055622:CDS	30.0%
!!	TTGTTCAAGATCGTTTTGAT+CGG	+	chr4.4:41442358-41442377	MS.gene055622:CDS	30.0%
	GTAAATCCAACATTCTCTCT+AGG	+	chr4.4:41442395-41442414	MS.gene055622:CDS	35.0%
	TGTAAACCTAGAGAGAATGT+TGG	-	chr4.4:41442404-41442423	None:intergenic	35.0%
!!	ATCGTTTTGATCGGAGATTC+TGG	+	chr4.4:41442367-41442386	MS.gene055622:CDS	40.0%
	TTGATCGGAGATTCTGGTGT+TGG	+	chr4.4:41442373-41442392	MS.gene055622:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	41442325	41442489	41442325	ID=MS.gene055622
chr4.4	mRNA	41442325	41442489	41442325	ID=MS.gene055622.t1;Parent=MS.gene055622
chr4.4	exon	41442325	41442489	41442325	ID=MS.gene055622.t1.exon1;Parent=MS.gene055622.t1
chr4.4	CDS	41442325	41442489	41442325	ID=cds.MS.gene055622.t1;Parent=MS.gene055622.t1

Gene Sequence	>MS.gene055622 ATGGCGCATAGGGTGGATCATGAGTATGATTACTTGTTCAAGATCGTTTTGATCGGAGATTCTGGTGTTGGTAAATCCAACATTCTCTCTAGGTTTACAAGAAATGAGTTTTGTTTGGAGTCTAAATCTACTATTGGTGTTGAATTCGCCACCAGAACCCTTCAG
Protein sequence	>MS.gene055622.t1 MAHRVDHEYDYLFKIVLIGDSGVGKSNILSRFTRNEFCLESKSTIGVEFATRTLQ