Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056425.t1 | XP_003628947.1 | 78.8 | 312 | 59 | 2 | 1 | 311 | 1 | 306 | 8.00E-100 | 374.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056425.t1 | Q9LR33 | 82.4 | 148 | 24 | 1 | 340 | 487 | 1 | 146 | 1.9e-49 | 198.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056425.t1 | G7LJC7 | 78.8 | 312 | 59 | 2 | 1 | 311 | 1 | 306 | 5.8e-100 | 374.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056425 | MS.gene062932 | 0.80461 | 1.99E-49 | -1.69E-46 |
| MS.gene056425 | MS.gene23359 | 0.808493 | 3.01E-50 | -1.69E-46 |
| MS.gene056425 | MS.gene35909 | 0.805042 | 1.62E-49 | -1.69E-46 |
| MS.gene056425 | MS.gene35910 | 0.811649 | 6.30E-51 | -1.69E-46 |
| MS.gene056425 | MS.gene49193 | 0.803749 | 3.00E-49 | -1.69E-46 |
| MS.gene056425 | MS.gene68819 | 0.823786 | 1.15E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056425.t1 | MTR_8g069450 | 88.344 | 326 | 31 | 2 | 1 | 325 | 1 | 320 | 0.0 | 551 |
| MS.gene056425.t1 | MTR_8g069450 | 90.805 | 261 | 17 | 2 | 1 | 260 | 1 | 255 | 2.51e-155 | 442 |
| MS.gene056425.t1 | MTR_8g069450 | 90.805 | 261 | 17 | 2 | 1 | 260 | 1 | 255 | 4.61e-155 | 441 |
| MS.gene056425.t1 | MTR_8g069460 | 99.324 | 148 | 1 | 0 | 340 | 487 | 1 | 148 | 3.30e-103 | 305 |
| MS.gene056425.t1 | MTR_5g033090 | 84.459 | 148 | 22 | 1 | 340 | 487 | 1 | 147 | 1.34e-82 | 252 |
| MS.gene056425.t1 | MTR_3g077050 | 83.784 | 148 | 23 | 1 | 340 | 487 | 1 | 147 | 4.07e-82 | 251 |
| MS.gene056425.t1 | MTR_4g119380 | 42.282 | 149 | 31 | 4 | 339 | 487 | 99 | 192 | 8.11e-21 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056425.t1 | AT3G26580 | 54.286 | 350 | 134 | 5 | 1 | 325 | 1 | 349 | 8.16e-119 | 352 |
| MS.gene056425.t1 | AT1G23290 | 83.108 | 148 | 23 | 1 | 340 | 487 | 1 | 146 | 6.31e-84 | 256 |
| MS.gene056425.t1 | AT1G70600 | 82.432 | 148 | 24 | 1 | 340 | 487 | 1 | 146 | 2.65e-83 | 254 |
| MS.gene056425.t1 | AT1G12960 | 51.701 | 147 | 24 | 2 | 340 | 486 | 1 | 100 | 1.59e-35 | 128 |
Find 131 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACCAAGATCTACATTATT+TGG | 0.191918 | 8.2:-33643791 | MS.gene056425:CDS |
| GCAATCCTTATGGCGATTAT+TGG | 0.215703 | 8.2:+33643605 | None:intergenic |
| GAAGAAAGCTGAAGAGTATT+TGG | 0.224413 | 8.2:-33645200 | MS.gene056425:CDS |
| AGTGATGAGGAGGGAGGGTT+AGG | 0.246902 | 8.2:-33645292 | MS.gene056425:CDS |
| TACGCAGTTTGGGTTCTTTA+AGG | 0.253757 | 8.2:-33638113 | MS.gene056425:CDS |
| TTTGGGTTCTTTAAGGTTCT+TGG | 0.257988 | 8.2:-33638106 | MS.gene056425:CDS |
| TGCAGAACTTCGTTACATTT+TGG | 0.260230 | 8.2:-33643523 | MS.gene056425:CDS |
| TGAAGAGTATTTGGAGAGTA+AGG | 0.278180 | 8.2:-33645191 | MS.gene056425:CDS |
| GTGAATTTCAGATACAAATA+TGG | 0.289294 | 8.2:-33643647 | MS.gene056425:intron |
| AGCTCAGTTGATGTATGATT+TGG | 0.292561 | 8.2:-33643882 | MS.gene056425:CDS |
| TTACGTGGCCGCGCTTCTTC+CGG | 0.302987 | 8.2:+33638395 | None:intergenic |
| TGGAGACCTCCTGCTGGATT+AGG | 0.310419 | 8.2:-33640632 | MS.gene056425:CDS |
| GTGATGAGGAGGGAGGGTTA+GGG | 0.320217 | 8.2:-33645291 | MS.gene056425:CDS |
| GATTGCTGAGAAGAAGATTA+AGG | 0.341259 | 8.2:-33638026 | MS.gene056425:CDS |
| ATCAAATGACCTGTCATAAC+TGG | 0.345451 | 8.2:+33643443 | None:intergenic |
| AAGAGAAGAGGATGAGAGTT+AGG | 0.351749 | 8.2:-33645141 | MS.gene056425:CDS |
| GTATAGTCACCATCTCTTCT+TGG | 0.352541 | 8.2:+33643478 | None:intergenic |
| ACAACAACACTCTGATTCTT+CGG | 0.371380 | 8.2:+33638070 | None:intergenic |
| CCGGCGTTTCCTCGGCCTCC+CGG | 0.376390 | 8.2:+33638334 | None:intergenic |
| GTGATTGATGTTACGCAGTT+TGG | 0.378054 | 8.2:-33638124 | MS.gene056425:CDS |
| CTCGGCCTCCCGGGTGTTTG+CGG | 0.382750 | 8.2:+33638344 | None:intergenic |
| CGCAAACACCCGGGAGGCCG+AGG | 0.385063 | 8.2:-33638343 | MS.gene056425:CDS |
| TCTTGGGTTATCTTGAGCTT+TGG | 0.386466 | 8.2:+33643495 | None:intergenic |
| TTGGGTTCTTTAAGGTTCTT+GGG | 0.390951 | 8.2:-33638105 | MS.gene056425:CDS |
| TGAAGATATGTCGAGGAGTA+TGG | 0.391046 | 8.2:+33645501 | None:intergenic |
| GGAGACCTCCTGCTGGATTA+GGG | 0.406684 | 8.2:-33640631 | MS.gene056425:CDS |
| CGGCGTTTCCTCGGCCTCCC+GGG | 0.408309 | 8.2:+33638335 | None:intergenic |
| TATAGTCACCATCTCTTCTT+GGG | 0.408773 | 8.2:+33643479 | None:intergenic |
| GGCCACGTAAGCGCCGGCCA+CGG | 0.410940 | 8.2:-33638382 | MS.gene056425:CDS |
| TTCTTTAAGGTTCTTGGGAA+AGG | 0.411341 | 8.2:-33638100 | MS.gene056425:CDS |
| CGTGCTGTTGAGTTTCTTGA+AGG | 0.415604 | 8.2:-33643827 | MS.gene056425:CDS |
| TTATCTTTAGATGATGGAAA+TGG | 0.418345 | 8.2:+33640674 | None:intergenic |
| CTGTCGAGAGTTAAGCTTCG+TGG | 0.419384 | 8.2:+33645478 | None:intergenic |
| CTTGTGTGGAGACCTCCTGC+TGG | 0.426709 | 8.2:-33640638 | MS.gene056425:CDS |
| GGAGGCACGTGGACGAGTGG+TGG | 0.426998 | 8.2:-33645368 | MS.gene056425:CDS |
| TGACGTTTGATGCTAATACT+AGG | 0.431805 | 8.2:+33643549 | None:intergenic |
| GCTCAGTTGATGTATGATTT+GGG | 0.432267 | 8.2:-33643881 | MS.gene056425:CDS |
| AAAGAGCAATGCAATCCTTA+TGG | 0.434951 | 8.2:+33643595 | None:intergenic |
| GGTTAGATTTCCTAAGCTTA+CGG | 0.437078 | 8.2:+33645522 | None:intergenic |
| CCACCAAATAATGTAGATCT+TGG | 0.439479 | 8.2:+33643788 | None:intergenic |
| AAGTTGAATTACAGCAGAAC+AGG | 0.440363 | 8.2:+33640227 | None:intergenic |
| TGATTGATGTTACGCAGTTT+GGG | 0.440404 | 8.2:-33638123 | MS.gene056425:CDS |
| AGTTAGGAAAGAGCTTGAAA+AGG | 0.442833 | 8.2:-33645125 | MS.gene056425:intron |
| GTTTGCGGTGTTTGCCGATA+CGG | 0.448429 | 8.2:+33638359 | None:intergenic |
| ATGGCTGGCTATGGCTTATG+AGG | 0.453962 | 8.2:-33643628 | MS.gene056425:CDS |
| GGACGAGTGGTGGTGGCTCG+TGG | 0.454291 | 8.2:-33645358 | MS.gene056425:CDS |
| GTCATAACTGGAACCAATCA+AGG | 0.454630 | 8.2:+33643455 | None:intergenic |
| GATTTGGGTAAGAAAGCTTA+CGG | 0.463969 | 8.2:-33643866 | MS.gene056425:CDS |
| TTTGCCGATACGGCCGTGGC+CGG | 0.467283 | 8.2:+33638369 | None:intergenic |
| TCCACCATGGCCGTAAGCTT+AGG | 0.469409 | 8.2:-33645532 | None:intergenic |
| GCGGTGGTGGTGCATTCCTC+CGG | 0.471622 | 8.2:+33638315 | None:intergenic |
| ATGGTGACTATACCCTTGAT+TGG | 0.471938 | 8.2:-33643468 | MS.gene056425:CDS |
| CCCACTTTGCCGAAGTAACC+AGG | 0.472874 | 8.2:+33638271 | None:intergenic |
| GGCACGTGGACGAGTGGTGG+TGG | 0.473510 | 8.2:-33645365 | MS.gene056425:CDS |
| GAGGAGGGAGGGTTAGGGGA+AGG | 0.478148 | 8.2:-33645286 | MS.gene056425:CDS |
| GCATTCCTCCGGCGTTTCCT+CGG | 0.478936 | 8.2:+33638326 | None:intergenic |
| ATTTGGGTAAGAAAGCTTAC+GGG | 0.482396 | 8.2:-33643865 | MS.gene056425:CDS |
| TGATGAGGAGGGAGGGTTAG+GGG | 0.489025 | 8.2:-33645290 | MS.gene056425:CDS |
| TGTTGTTGTTAAAGCGAAGC+TGG | 0.489814 | 8.2:-33638056 | MS.gene056425:CDS |
| GGTAAGAAAGCTTACGGGAG+AGG | 0.493146 | 8.2:-33643860 | MS.gene056425:CDS |
| TTGGAGAGTAAGGGTGATGA+TGG | 0.493963 | 8.2:-33645181 | MS.gene056425:CDS |
| GCCTTGTCCTTCACTTCTTG+AGG | 0.494689 | 8.2:+33638172 | None:intergenic |
| GACGTTTGATGCTAATACTA+GGG | 0.495643 | 8.2:+33643550 | None:intergenic |
| AGCGCCGGCCACGGCCGTAT+CGG | 0.496756 | 8.2:-33638373 | MS.gene056425:CDS |
| ATTCAACTTCAACCCTCCTC+AGG | 0.496898 | 8.2:-33640213 | MS.gene056425:intron |
| ATTTCCTAAGCTTACGGCCA+TGG | 0.497052 | 8.2:+33645528 | None:intergenic |
| ATCTACATTATTTGGTGGTG+AGG | 0.501114 | 8.2:-33643783 | MS.gene056425:intron |
| GGCCGTGGCCGGCGCTTACG+TGG | 0.503432 | 8.2:+33638380 | None:intergenic |
| CAGAGAGTGATGAGGAGGGA+GGG | 0.507705 | 8.2:-33645297 | MS.gene056425:CDS |
| AAGCGCGGCCACGTAAGCGC+CGG | 0.513198 | 8.2:-33638388 | MS.gene056425:CDS |
| CCGGGAGGCCGAGGAAACGC+CGG | 0.514148 | 8.2:-33638334 | MS.gene056425:CDS |
| CGGCAAACACCGCAAACACC+CGG | 0.519441 | 8.2:-33638353 | MS.gene056425:CDS |
| TGATTGGTTCCAGTTATGAC+AGG | 0.524132 | 8.2:-33643452 | MS.gene056425:intron |
| GAATCAGGTGGCTAAGGAGC+AGG | 0.526095 | 8.2:-33643921 | MS.gene056425:intron |
| CCAAGATCTACATTATTTGG+TGG | 0.527272 | 8.2:-33643788 | MS.gene056425:CDS |
| TCAGAGAGTGATGAGGAGGG+AGG | 0.531390 | 8.2:-33645298 | MS.gene056425:CDS |
| AAGAGAGTGAGGAAGAGAAG+AGG | 0.534892 | 8.2:-33645153 | MS.gene056425:CDS |
| CCGTGAACATCGATACTCTC+TGG | 0.537090 | 8.2:-33638204 | MS.gene056425:CDS |
| TAAGGAGCAGGCAGAGAGGA+GGG | 0.539752 | 8.2:-33643909 | MS.gene056425:CDS |
| TGTCGAAGAGGATGCGGTGG+TGG | 0.540799 | 8.2:+33638302 | None:intergenic |
| CCAGAGAGTATCGATGTTCA+CGG | 0.544302 | 8.2:+33638204 | None:intergenic |
| CCCTGGTTACTTCGGCAAAG+TGG | 0.546405 | 8.2:-33638272 | MS.gene056425:CDS |
| TGAGGCCAATAATCGCCATA+AGG | 0.547038 | 8.2:-33643610 | MS.gene056425:CDS |
| TGATTCAGAGAGTGATGAGG+AGG | 0.547421 | 8.2:-33645302 | MS.gene056425:CDS |
| GACGAGTGGTGGTGGCTCGT+GGG | 0.547546 | 8.2:-33645357 | MS.gene056425:CDS |
| CCTGGTTACTTCGGCAAAGT+GGG | 0.551584 | 8.2:-33638271 | MS.gene056425:CDS |
| TAATGTAGATCTTGGTATGA+TGG | 0.552370 | 8.2:+33643796 | None:intergenic |
| ATTTCAGATACAAATATGGC+TGG | 0.553752 | 8.2:-33643643 | MS.gene056425:intron |
| GAAGAGTATTTGGAGAGTAA+GGG | 0.556657 | 8.2:-33645190 | MS.gene056425:CDS |
| AGCATAACTACAACAGCAAG+AGG | 0.558274 | 8.2:+33645399 | None:intergenic |
| AAAGATAAGGAGGAGAGTGA+AGG | 0.561760 | 8.2:-33645248 | MS.gene056425:CDS |
| TGATGGTGAGGAAGAGAGTG+AGG | 0.565673 | 8.2:-33645164 | MS.gene056425:CDS |
| AAGTATCACCCTGGTTACTT+CGG | 0.566184 | 8.2:-33638280 | MS.gene056425:CDS |
| GATACAAATATGGCTGGCTA+TGG | 0.569208 | 8.2:-33643637 | MS.gene056425:CDS |
| TCTGTAGTTATGCGGGAACA+TGG | 0.570481 | 8.2:-33640739 | MS.gene056425:intron |
| GAGTGAAGGAGTTTGAAGAG+AGG | 0.570724 | 8.2:-33645234 | MS.gene056425:CDS |
| GATGAAGAGTCTCTCTTGAG+AGG | 0.573372 | 8.2:+33645436 | None:intergenic |
| AAGTCAAACTCGGAGCAGAA+TGG | 0.574039 | 8.2:-33645334 | MS.gene056425:CDS |
| CGATGTTCACGGTGGGACAG+TGG | 0.575870 | 8.2:+33638215 | None:intergenic |
| GAGAAGAAGATTAAGGAAGC+TGG | 0.577905 | 8.2:-33638019 | MS.gene056425:CDS |
| CAAGATAACCCAAGAAGAGA+TGG | 0.579385 | 8.2:-33643487 | MS.gene056425:CDS |
| GATACTTGTCGAAGAGGATG+CGG | 0.579398 | 8.2:+33638296 | None:intergenic |
| GCTTACGGGAGAGGCACGTA+CGG | 0.581433 | 8.2:-33643851 | MS.gene056425:CDS |
| GGCTCGTGGGAAGTCAAACT+CGG | 0.583210 | 8.2:-33645344 | MS.gene056425:CDS |
| GATTCAGAGAGTGATGAGGA+GGG | 0.583330 | 8.2:-33645301 | MS.gene056425:CDS |
| CTAAGGAGCAGGCAGAGAGG+AGG | 0.584864 | 8.2:-33643910 | MS.gene056425:CDS |
| GGTGTTTGCCGATACGGCCG+TGG | 0.585895 | 8.2:+33638365 | None:intergenic |
| TGGGACAGTGGAATTTGTTG+CGG | 0.598138 | 8.2:+33638227 | None:intergenic |
| TTTGACGAATCAGGTGGCTA+AGG | 0.599329 | 8.2:-33643927 | MS.gene056425:intron |
| TTGAGAGGTAACGTGAGAAG+AGG | 0.599921 | 8.2:+33645451 | None:intergenic |
| TCTTCTACCTCAAGAAGTGA+AGG | 0.601537 | 8.2:-33638179 | MS.gene056425:CDS |
| CAGGGTGATACTTGTCGAAG+AGG | 0.610306 | 8.2:+33638290 | None:intergenic |
| GGAGGCCGAGGAAACGCCGG+AGG | 0.613959 | 8.2:-33638331 | MS.gene056425:CDS |
| GGACAAGGCATCAAAGTCGA+AGG | 0.615129 | 8.2:-33638158 | MS.gene056425:CDS |
| GAGTAAGGGTGATGATGGTG+AGG | 0.618593 | 8.2:-33645176 | MS.gene056425:CDS |
| GGCAAACACCGCAAACACCC+GGG | 0.623758 | 8.2:-33638352 | MS.gene056425:CDS |
| AACTGCGTAACATCAATCAC+AGG | 0.626930 | 8.2:+33638127 | None:intergenic |
| TCATAACTGGAACCAATCAA+GGG | 0.627632 | 8.2:+33643456 | None:intergenic |
| AGAGTATCGATGTTCACGGT+GGG | 0.631980 | 8.2:+33638208 | None:intergenic |
| CTTTGATTCAGAGAGTGATG+AGG | 0.635451 | 8.2:-33645305 | MS.gene056425:CDS |
| CTCTTCGACAAGTATCACCC+TGG | 0.650081 | 8.2:-33638289 | MS.gene056425:CDS |
| ATCATACATCAACTGAGCTG+TGG | 0.650968 | 8.2:+33643886 | None:intergenic |
| AAACTCGGAGCAGAATGGAG+TGG | 0.664807 | 8.2:-33645329 | MS.gene056425:CDS |
| TGGCTAAGGAGCAGGCAGAG+AGG | 0.675876 | 8.2:-33643913 | MS.gene056425:CDS |
| TTCGTGGTGAAGATATGTCG+AGG | 0.678789 | 8.2:+33645494 | None:intergenic |
| CCACTTTGCCGAAGTAACCA+GGG | 0.680564 | 8.2:+33638272 | None:intergenic |
| AAACACCGCAAACACCCGGG+AGG | 0.687508 | 8.2:-33638349 | MS.gene056425:CDS |
| AAGAAGATTAAGGAAGCTGG+TGG | 0.688253 | 8.2:-33638016 | MS.gene056425:CDS |
| ACTTGTCGAAGAGGATGCGG+TGG | 0.693301 | 8.2:+33638299 | None:intergenic |
| ACCTCAAGAAGTGAAGGACA+AGG | 0.699258 | 8.2:-33638173 | MS.gene056425:CDS |
| GAGAGTATCGATGTTCACGG+TGG | 0.722248 | 8.2:+33638207 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 33637994 | 33645548 | 33637994 | ID=MS.gene056425 |
| chr8.2 | mRNA | 33637994 | 33645548 | 33637994 | ID=MS.gene056425.t1;Parent=MS.gene056425 |
| chr8.2 | exon | 33645126 | 33645548 | 33645126 | ID=MS.gene056425.t1.exon1;Parent=MS.gene056425.t1 |
| chr8.2 | CDS | 33645126 | 33645548 | 33645126 | ID=cds.MS.gene056425.t1;Parent=MS.gene056425.t1 |
| chr8.2 | exon | 33643784 | 33643936 | 33643784 | ID=MS.gene056425.t1.exon2;Parent=MS.gene056425.t1 |
| chr8.2 | CDS | 33643784 | 33643936 | 33643784 | ID=cds.MS.gene056425.t1;Parent=MS.gene056425.t1 |
| chr8.2 | exon | 33643453 | 33643658 | 33643453 | ID=MS.gene056425.t1.exon3;Parent=MS.gene056425.t1 |
| chr8.2 | CDS | 33643453 | 33643658 | 33643453 | ID=cds.MS.gene056425.t1;Parent=MS.gene056425.t1 |
| chr8.2 | exon | 33640609 | 33640754 | 33640609 | ID=MS.gene056425.t1.exon4;Parent=MS.gene056425.t1 |
| chr8.2 | CDS | 33640609 | 33640754 | 33640609 | ID=cds.MS.gene056425.t1;Parent=MS.gene056425.t1 |
| chr8.2 | exon | 33640214 | 33640270 | 33640214 | ID=MS.gene056425.t1.exon5;Parent=MS.gene056425.t1 |
| chr8.2 | CDS | 33640214 | 33640270 | 33640214 | ID=cds.MS.gene056425.t1;Parent=MS.gene056425.t1 |
| chr8.2 | exon | 33637994 | 33638472 | 33637994 | ID=MS.gene056425.t1.exon6;Parent=MS.gene056425.t1 |
| chr8.2 | CDS | 33637994 | 33638472 | 33637994 | ID=cds.MS.gene056425.t1;Parent=MS.gene056425.t1 |
| Gene Sequence |
| Protein sequence |
