Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68819.t1 | GAU45956.1 | 93.2 | 146 | 9 | 1 | 1 | 145 | 7 | 152 | 1.40E-68 | 268.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68819.t1 | Q9SY29 | 61.9 | 134 | 46 | 2 | 1 | 133 | 224 | 353 | 3.2e-40 | 166.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68819.t1 | A0A2Z6NQR6 | 93.2 | 146 | 9 | 1 | 1 | 145 | 7 | 152 | 1.0e-68 | 268.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene68819 | 0.811397 | 7.15E-51 | -1.69E-46 |
| MS.gene049153 | MS.gene68819 | 0.802039 | 6.78E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene68819 | 0.821697 | 3.52E-53 | -1.69E-46 |
| MS.gene049378 | MS.gene68819 | 0.806572 | 7.71E-50 | -1.69E-46 |
| MS.gene049443 | MS.gene68819 | 0.804889 | 1.74E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene68819 | 0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene049910 | MS.gene68819 | 0.825578 | 4.34E-54 | -1.69E-46 |
| MS.gene049915 | MS.gene68819 | 0.855395 | 6.50E-62 | -1.69E-46 |
| MS.gene050168 | MS.gene68819 | 0.80747 | 4.98E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene68819 | 0.838679 | 2.49E-57 | -1.69E-46 |
| MS.gene050227 | MS.gene68819 | 0.8305 | 2.84E-55 | -1.69E-46 |
| MS.gene050228 | MS.gene68819 | 0.833096 | 6.49E-56 | -1.69E-46 |
| MS.gene050398 | MS.gene68819 | 0.839758 | 1.31E-57 | -1.69E-46 |
| MS.gene050642 | MS.gene68819 | 0.822317 | 2.53E-53 | -1.69E-46 |
| MS.gene050820 | MS.gene68819 | 0.813642 | 2.31E-51 | -1.69E-46 |
| MS.gene050987 | MS.gene68819 | 0.800603 | 1.33E-48 | -1.69E-46 |
| MS.gene051349 | MS.gene68819 | 0.835174 | 1.96E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene68819 | 0.815827 | 7.57E-52 | -1.69E-46 |
| MS.gene052176 | MS.gene68819 | 0.831066 | 2.06E-55 | -1.69E-46 |
| MS.gene052255 | MS.gene68819 | 0.843012 | 1.82E-58 | -1.69E-46 |
| MS.gene052302 | MS.gene68819 | 0.807402 | 5.14E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene68819 | 0.804397 | 2.20E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene68819 | 0.823734 | 1.18E-53 | -1.69E-46 |
| MS.gene052426 | MS.gene68819 | 0.83051 | 2.82E-55 | -1.69E-46 |
| MS.gene052902 | MS.gene68819 | 0.803881 | 2.82E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene68819 | 0.811499 | 6.79E-51 | -1.69E-46 |
| MS.gene053184 | MS.gene68819 | 0.81785 | 2.66E-52 | -1.69E-46 |
| MS.gene053494 | MS.gene68819 | 0.810054 | 1.39E-50 | -1.69E-46 |
| MS.gene053584 | MS.gene68819 | 0.801092 | 1.06E-48 | -1.69E-46 |
| MS.gene053836 | MS.gene68819 | 0.823322 | 1.47E-53 | -1.69E-46 |
| MS.gene054184 | MS.gene68819 | 0.819156 | 1.35E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene68819 | 0.825149 | 5.49E-54 | -1.69E-46 |
| MS.gene054542 | MS.gene68819 | 0.806014 | 1.01E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene68819 | 0.848129 | 7.46E-60 | -1.69E-46 |
| MS.gene05510 | MS.gene68819 | 0.828401 | 9.18E-55 | -1.69E-46 |
| MS.gene055133 | MS.gene68819 | 0.806745 | 7.09E-50 | -1.69E-46 |
| MS.gene056425 | MS.gene68819 | 0.823786 | 1.15E-53 | -1.69E-46 |
| MS.gene056506 | MS.gene68819 | 0.801342 | 9.42E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene68819 | 0.813227 | 2.85E-51 | -1.69E-46 |
| MS.gene057121 | MS.gene68819 | 0.805762 | 1.14E-49 | -1.69E-46 |
| MS.gene057750 | MS.gene68819 | 0.863757 | 1.98E-64 | -1.69E-46 |
| MS.gene057754 | MS.gene68819 | 0.889241 | 2.89E-73 | -1.69E-46 |
| MS.gene058002 | MS.gene68819 | 0.807443 | 5.04E-50 | -1.69E-46 |
| MS.gene058372 | MS.gene68819 | 0.810334 | 1.21E-50 | -1.69E-46 |
| MS.gene058749 | MS.gene68819 | 0.816019 | 6.86E-52 | -1.69E-46 |
| MS.gene058833 | MS.gene68819 | 0.812047 | 5.16E-51 | -1.69E-46 |
| MS.gene059124 | MS.gene68819 | 0.893746 | 4.71E-75 | -1.69E-46 |
| MS.gene059128 | MS.gene68819 | 0.820287 | 7.43E-53 | -1.69E-46 |
| MS.gene059285 | MS.gene68819 | 0.810388 | 1.18E-50 | -1.69E-46 |
| MS.gene059421 | MS.gene68819 | 0.829922 | 3.92E-55 | -1.69E-46 |
| MS.gene060255 | MS.gene68819 | 0.802303 | 5.98E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene68819 | 0.802693 | 4.97E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene68819 | 0.827154 | 1.83E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene68819 | 0.805308 | 1.42E-49 | -1.69E-46 |
| MS.gene061085 | MS.gene68819 | 0.808832 | 2.55E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68819.t1 | MTR_2g437590 | 94.558 | 147 | 8 | 0 | 1 | 147 | 268 | 414 | 1.42e-98 | 289 |
| MS.gene68819.t1 | MTR_2g437580 | 83.688 | 141 | 22 | 1 | 1 | 141 | 249 | 388 | 6.66e-80 | 241 |
| MS.gene68819.t1 | MTR_3g099740 | 37.903 | 124 | 76 | 1 | 1 | 124 | 232 | 354 | 6.22e-21 | 87.4 |
| MS.gene68819.t1 | MTR_5g065080 | 34.507 | 142 | 84 | 2 | 1 | 142 | 230 | 362 | 8.29e-21 | 87.0 |
| MS.gene68819.t1 | MTR_3g099750 | 35.593 | 118 | 75 | 1 | 7 | 124 | 237 | 353 | 7.50e-18 | 79.0 |
| MS.gene68819.t1 | MTR_7g080750 | 29.323 | 133 | 90 | 2 | 4 | 133 | 220 | 351 | 2.01e-17 | 77.4 |
| MS.gene68819.t1 | MTR_2g015470 | 31.818 | 132 | 84 | 2 | 7 | 138 | 252 | 377 | 5.30e-16 | 73.9 |
| MS.gene68819.t1 | MTR_6g082540 | 33.582 | 134 | 81 | 5 | 4 | 135 | 42 | 169 | 5.30e-15 | 68.6 |
| MS.gene68819.t1 | MTR_3g058660 | 34.328 | 134 | 80 | 5 | 4 | 135 | 224 | 351 | 1.22e-13 | 67.0 |
| MS.gene68819.t1 | MTR_8g024620 | 27.434 | 113 | 81 | 1 | 1 | 113 | 250 | 361 | 3.85e-11 | 60.1 |
| MS.gene68819.t1 | MTR_8g024620 | 27.434 | 113 | 81 | 1 | 1 | 113 | 251 | 362 | 3.85e-11 | 60.1 |
| MS.gene68819.t1 | MTR_8g024620 | 27.434 | 113 | 81 | 1 | 1 | 113 | 239 | 350 | 4.25e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68819.t1 | AT1G30840 | 65.789 | 114 | 38 | 1 | 1 | 113 | 224 | 337 | 9.32e-47 | 155 |
| MS.gene68819.t1 | AT1G30840 | 65.789 | 114 | 38 | 1 | 1 | 113 | 224 | 337 | 9.32e-47 | 155 |
| MS.gene68819.t1 | AT1G28230 | 32.877 | 146 | 94 | 1 | 1 | 146 | 215 | 356 | 2.16e-19 | 83.2 |
| MS.gene68819.t1 | AT1G28220 | 36.000 | 125 | 78 | 2 | 1 | 125 | 209 | 331 | 8.37e-19 | 81.3 |
| MS.gene68819.t1 | AT2G33750 | 36.290 | 124 | 76 | 3 | 1 | 124 | 211 | 331 | 4.99e-16 | 73.6 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 171 | 288 | 1.24e-15 | 72.4 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 171 | 288 | 1.24e-15 | 72.4 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 171 | 288 | 1.24e-15 | 72.4 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 171 | 288 | 1.24e-15 | 72.4 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 171 | 288 | 1.24e-15 | 72.4 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 247 | 364 | 1.49e-15 | 72.4 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 235 | 352 | 1.57e-15 | 72.4 |
| MS.gene68819.t1 | AT1G44750 | 31.092 | 119 | 81 | 1 | 4 | 122 | 235 | 352 | 1.57e-15 | 72.4 |
| MS.gene68819.t1 | AT2G33750 | 34.328 | 134 | 76 | 4 | 1 | 124 | 211 | 342 | 1.65e-13 | 66.6 |
| MS.gene68819.t1 | AT4G18190 | 31.783 | 129 | 85 | 2 | 5 | 131 | 247 | 374 | 1.75e-12 | 63.9 |
| MS.gene68819.t1 | AT4G18210 | 28.777 | 139 | 92 | 5 | 1 | 135 | 246 | 381 | 2.95e-11 | 60.5 |
| MS.gene68819.t1 | AT4G18220 | 32.759 | 116 | 73 | 4 | 3 | 116 | 248 | 360 | 5.07e-11 | 59.7 |
Find 0 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAGGAAAGTAGAGTTTAT+TGG | - | 15233:13134-13153 | MS.gene68819:CDS | 25.0% |
| ! | GTTGTTTATAGAGATGAATT+TGG | - | 15233:13292-13311 | MS.gene68819:CDS | 25.0% |
| ! | TAAGGAAAGTAGAGTTTATT+GGG | - | 15233:13135-13154 | MS.gene68819:CDS | 25.0% |
| !! | GGATTTTGTTCTTATGTTTA+TGG | - | 15233:13352-13371 | MS.gene68819:CDS | 25.0% |
| GTTTATAGAGATGAATTTGG+TGG | - | 15233:13295-13314 | MS.gene68819:CDS | 30.0% | |
| TGGTATGTATGTTAAGATGT+TGG | - | 15233:13372-13391 | MS.gene68819:CDS | 30.0% | |
| TTAAGATGTTGGAAGAGAAA+GGG | - | 15233:13383-13402 | MS.gene68819:CDS | 30.0% | |
| TTCTTGACATCTTCATTAAC+TGG | - | 15233:13220-13239 | MS.gene68819:CDS | 30.0% | |
| TTGTTGTCTATGAATGTGTT+GGG | - | 15233:13262-13281 | MS.gene68819:CDS | 30.0% | |
| ! | TTCAACTGTTTTGTGTATGT+GGG | - | 15233:13330-13349 | MS.gene68819:CDS | 30.0% |
| ! | TTTCAACTGTTTTGTGTATG+TGG | - | 15233:13329-13348 | MS.gene68819:CDS | 30.0% |
| ! | TTTGTTGTCTATGAATGTGT+TGG | - | 15233:13261-13280 | MS.gene68819:CDS | 30.0% |
| AAGTAGAGTTTATTGGGTGA+CGG | - | 15233:13141-13160 | MS.gene68819:CDS | 35.0% | |
| AGAAATGAAGAGAGAAAGTG+AGG | - | 15233:13102-13121 | MS.gene68819:CDS | 35.0% | |
| GTTAAGATGTTGGAAGAGAA+AGG | - | 15233:13382-13401 | MS.gene68819:CDS | 35.0% | |
| TGACATCTTCATTAACTGGT+GGG | - | 15233:13224-13243 | MS.gene68819:CDS | 35.0% | |
| TTGACATCTTCATTAACTGG+TGG | - | 15233:13223-13242 | MS.gene68819:CDS | 35.0% | |
| TTGAGTACTATGAGAAGAAG+TGG | - | 15233:13442-13461 | MS.gene68819:CDS | 35.0% | |
| ! | AAGTGAGGAGGTTTTTGATA+AGG | - | 15233:13117-13136 | MS.gene68819:CDS | 35.0% |
| ! | TCAACTGTTTTGTGTATGTG+GGG | - | 15233:13331-13350 | MS.gene68819:CDS | 35.0% |
| !!! | ACGTGGCAGTTTTGTTTTAT+GGG | - | 15233:13181-13200 | MS.gene68819:CDS | 35.0% |
| AAGGGAAGATCGTTAAGGAA+AGG | - | 15233:13401-13420 | MS.gene68819:CDS | 40.0% | |
| AATGAAGAGAGAAAGTGAGG+AGG | - | 15233:13105-13124 | MS.gene68819:CDS | 40.0% | |
| AGAGAAAGGGAAGATCGTTA+AGG | - | 15233:13396-13415 | MS.gene68819:CDS | 40.0% | |
| AGTACTATGAGAAGAAGTGG+AGG | - | 15233:13445-13464 | MS.gene68819:CDS | 40.0% | |
| GGAAAGGAATGATTCTTCCA+TGG | - | 15233:13417-13436 | MS.gene68819:CDS | 40.0% | |
| TCTCATAGTACTCAACTCCA+TGG | + | 15233:13437-13456 | MS.gene68819:intergenic | 40.0% | |
| TTGTCTATGAATGTGTTGGG+TGG | - | 15233:13265-13284 | MS.gene68819:CDS | 40.0% | |
| ! | AGTTTATTGGGTGACGGTTA+TGG | - | 15233:13147-13166 | MS.gene68819:CDS | 40.0% |
| ! | TATAATGGAGATTGCAGCGA+CGG | - | 15233:13039-13058 | MS.gene68819:CDS | 40.0% |
| !!! | CGTGGCAGTTTTGTTTTATG+GGG | - | 15233:13182-13201 | MS.gene68819:CDS | 40.0% |
| !!! | GACGTGGCAGTTTTGTTTTA+TGG | - | 15233:13180-13199 | MS.gene68819:CDS | 40.0% |
| !!! | TTTGTTTTATGGGGACTGCT+GGG | - | 15233:13191-13210 | MS.gene68819:CDS | 40.0% |
| !!! | TTTTGTTTTATGGGGACTGC+TGG | - | 15233:13190-13209 | MS.gene68819:CDS | 40.0% |
| TTATGGCGAATGTCGTGACG+TGG | - | 15233:13164-13183 | MS.gene68819:CDS | 50.0% | |
| ! | TTTTATGGGGACTGCTGGGA+TGG | - | 15233:13195-13214 | MS.gene68819:CDS | 50.0% |
| !! | GGTTTTAGCGACAGTGGGAA+TGG | - | 15233:13060-13079 | MS.gene68819:CDS | 50.0% |
| ! | AGAAGAAGTGGAGGTGCAGC+TGG | - | 15233:13454-13473 | MS.gene68819:CDS | 55.0% |
| !! | AGCGACGGTTTTAGCGACAG+TGG | - | 15233:13054-13073 | MS.gene68819:CDS | 55.0% |
| !! | GCGACGGTTTTAGCGACAGT+GGG | - | 15233:13055-13074 | MS.gene68819:CDS | 55.0% |
| ACAGTGGGAATGGCGTGGGA+CGG | - | 15233:13070-13089 | MS.gene68819:CDS | 60.0% | |
| AGCGACAGTGGGAATGGCGT+GGG | - | 15233:13066-13085 | MS.gene68819:CDS | 60.0% | |
| TAGCGACAGTGGGAATGGCG+TGG | - | 15233:13065-13084 | MS.gene68819:CDS | 60.0% | |
| GTGGGAATGGCGTGGGACGG+CGG | - | 15233:13073-13092 | MS.gene68819:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15233 | gene | 13037 | 13480 | 13037 | ID=MS.gene68819 |
| 15233 | mRNA | 13037 | 13480 | 13037 | ID=MS.gene68819.t1;Parent=MS.gene68819 |
| 15233 | exon | 13037 | 13480 | 13037 | ID=MS.gene68819.t1.exon1;Parent=MS.gene68819.t1 |
| 15233 | CDS | 13037 | 13480 | 13037 | ID=cds.MS.gene68819.t1;Parent=MS.gene68819.t1 |
| Gene Sequence |
| Protein sequence |
