AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35910.t1 MTR_5g072780 95.392 217 10 0 1 217 1 217 1.71e-148 412
MS.gene35910.t1 MTR_7g032240 39.850 133 41 3 123 216 219 351 2.72e-20 87.8
MS.gene35910.t1 MTR_1g110870 44.144 111 42 3 124 216 316 424 1.49e-19 86.3
MS.gene35910.t1 MTR_4g127420 53.488 86 36 2 129 210 293 378 3.39e-19 85.1
MS.gene35910.t1 MTR_8g104190 41.727 139 53 4 94 216 274 400 2.69e-18 82.8
MS.gene35910.t1 MTR_1g013450 36.441 118 62 3 106 216 186 297 1.30e-13 68.9
MS.gene35910.t1 MTR_3g105710 67.442 43 14 0 174 216 232 274 1.95e-11 62.4
MS.gene35910.t1 MTR_4g128930 67.442 43 14 0 174 216 298 340 3.91e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35910.t1 AT1G07050 67.000 100 26 3 119 216 99 193 3.16e-37 129
MS.gene35910.t1 AT1G07050 64.894 94 26 3 119 210 99 187 2.87e-32 116
MS.gene35910.t1 AT5G14370 53.211 109 36 3 123 216 228 336 2.95e-28 109
MS.gene35910.t1 AT4G25990 53.922 102 39 3 119 216 286 383 1.43e-23 97.4
MS.gene35910.t1 AT5G57180 53.846 91 40 2 126 216 337 425 6.70e-23 95.9
MS.gene35910.t1 AT1G73870 42.553 141 57 5 94 216 253 387 4.66e-21 90.5
MS.gene35910.t1 AT4G25990 47.009 117 39 4 119 216 286 398 8.08e-20 87.0
MS.gene35910.t1 AT4G25990 52.083 96 38 3 119 210 286 377 2.03e-19 85.9
MS.gene35910.t1 AT1G49130 52.747 91 34 3 128 216 230 313 2.04e-19 85.1
MS.gene35910.t1 AT1G49130 52.747 91 34 3 128 216 237 320 2.13e-19 85.1
MS.gene35910.t1 AT1G25440 46.429 112 42 4 123 216 292 403 4.38e-19 85.1
MS.gene35910.t1 AT1G68520 41.525 118 51 3 117 216 282 399 9.82e-19 84.0
MS.gene35910.t1 AT5G57180 51.765 85 39 2 126 210 337 419 1.11e-18 84.0
MS.gene35910.t1 AT2G24790 61.111 54 21 0 163 216 218 271 3.73e-13 67.4
MS.gene35910.t1 AT5G24930 53.226 62 29 0 155 216 276 337 5.38e-12 64.3
MS.gene35910.t1 AT5G57660 69.767 43 13 0 174 216 285 327 1.23e-11 63.5

Find 34 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCATGTTCTTCTTTCTT+TGG 0.183818 5.1:-55922196 None:intergenic
GGTCTGACCGTGGCTCTCTT+TGG 0.244079 5.1:+55922578 MS.gene35910:CDS
GCTCTCTCTTCTTCCAATAT+TGG 0.250193 5.1:-55923381 None:intergenic
TTTGAATTATCAAGAGGTTT+TGG 0.259887 5.1:+55922549 MS.gene35910:CDS
AAAAGTGTGACCTTGATTCT+TGG 0.281388 5.1:-55923507 None:intergenic
GAAGAGAAGCAAGTGTTCTT+AGG 0.336563 5.1:+55923406 MS.gene35910:CDS
GTCTGACCGTGGCTCTCTTT+GGG 0.337400 5.1:+55922579 MS.gene35910:CDS
GATGGGAGAAGTGCCAATAT+TGG 0.355037 5.1:+55923368 MS.gene35910:intron
AGTGTAAGATTTGTTGAAGT+TGG 0.374346 5.1:-55922245 None:intergenic
ATAGTAGTAATGAGCTTCAT+TGG 0.401933 5.1:+55922371 MS.gene35910:CDS
CTCATCTTTCCCTTCATCAA+AGG 0.415253 5.1:-55922419 None:intergenic
TCATCTTTCCCTTCATCAAA+GGG 0.428053 5.1:-55922418 None:intergenic
AAGAGAGAAAATTACAATGA+TGG 0.429279 5.1:+55922481 MS.gene35910:CDS
CATAAGCAAATGCAATTATG+AGG 0.471927 5.1:+55922444 MS.gene35910:CDS
TGAGCTTCATTGGGATATCA+TGG 0.472007 5.1:+55922381 MS.gene35910:CDS
GAGGGATTTCCCTTTGATGA+AGG 0.484035 5.1:+55922409 MS.gene35910:CDS
ACTAACATAGGAGCCACTAT+TGG 0.497136 5.1:-55922632 None:intergenic
TAGTAGTAATGAGCTTCATT+GGG 0.508412 5.1:+55922372 MS.gene35910:CDS
AGGGATTTCCCTTTGATGAA+GGG 0.520507 5.1:+55922410 MS.gene35910:CDS
TCATTGGGATATCATGGAAT+GGG 0.534580 5.1:+55922387 MS.gene35910:CDS
TTCATTGGGATATCATGGAA+TGG 0.539426 5.1:+55922386 MS.gene35910:CDS
GGTATTTGAGTGGTAGCGTC+TGG 0.540746 5.1:-55922220 None:intergenic
TTTGAATTTGAATTATCAAG+AGG 0.541289 5.1:+55922543 MS.gene35910:CDS
AACATAGGAGCCACTATTGG+AGG 0.541652 5.1:-55922629 None:intergenic
CGCTTTGTGAAGAGACATTG+AGG 0.551371 5.1:+55923635 MS.gene35910:CDS
TAAGATTTGTTGAAGTTGGA+TGG 0.551571 5.1:-55922241 None:intergenic
TGATAAAAGACCAAGAATCA+AGG 0.578251 5.1:+55923497 MS.gene35910:CDS
ATAGGAGCCACTATTGGAGG+AGG 0.606351 5.1:-55922626 None:intergenic
TTGGGATATCATGGAATGGG+AGG 0.609318 5.1:+55922390 MS.gene35910:CDS
GAAGTTGGATGGTATTTGAG+TGG 0.615620 5.1:-55922230 None:intergenic
TGGGATATCATGGAATGGGA+GGG 0.659236 5.1:+55922391 MS.gene35910:CDS
CATTAGCCCAAAGAGAGCCA+CGG 0.677020 5.1:-55922585 None:intergenic
TTGGATGCATGGTCTGACCG+TGG 0.694015 5.1:+55922568 MS.gene35910:CDS
AATGGATGATGAAAAGAGTG+TGG 0.704180 5.1:+55922513 MS.gene35910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATAGTTGTTATA+TGG - chr5.1:55922786-55922805 None:intergenic 10.0%
!!! TAATTTTAAATAGTAACAAA+AGG - chr5.1:55922818-55922837 None:intergenic 10.0%
!! TAAACAAAAAAGAAATTACT+AGG - chr5.1:55923348-55923367 None:intergenic 15.0%
!!! AATTTCTTTTTTGTTTAGAT+GGG + chr5.1:55923351-55923370 MS.gene35910:intron 15.0%
!!! GTTTCTTTTTTTTTTTCATT+GGG + chr5.1:55923551-55923570 MS.gene35910:intron 15.0%
!!! TAATTTCTTTTTTGTTTAGA+TGG + chr5.1:55923350-55923369 MS.gene35910:intron 15.0%
!!! TATCAATTCTTATTCTTTTA+AGG + chr5.1:55922735-55922754 MS.gene35910:intron 15.0%
!!! TGTTTCTTTTTTTTTTTCAT+TGG + chr5.1:55923550-55923569 MS.gene35910:intron 15.0%
!! ATATAACAACTTATCCAAAA+AGG + chr5.1:55922760-55922779 MS.gene35910:intron 20.0%
!! TTTGAATTTGAATTATCAAG+AGG + chr5.1:55922543-55922562 MS.gene35910:CDS 20.0%
!!! AAATTACAATGATGGTTTTT+TGG + chr5.1:55922489-55922508 MS.gene35910:CDS 20.0%
!!! ATAACTAAATTTTTGGTGAT+AGG + chr5.1:55923099-55923118 MS.gene35910:intron 20.0%
!!! GTAACATATTTAGTTCATTT+TGG + chr5.1:55923195-55923214 MS.gene35910:intron 20.0%
!!! TATGTTGATTTTTGTTTAGA+TGG + chr5.1:55922980-55922999 MS.gene35910:intron 20.0%
!!! TTTGTCGATAACTAAATTTT+TGG + chr5.1:55923092-55923111 MS.gene35910:intron 20.0%
!!! TTTGTGATATTTTTGATGTA+GGG + chr5.1:55923611-55923630 MS.gene35910:intron 20.0%
!!! TTTTGTGATATTTTTGATGT+AGG + chr5.1:55923610-55923629 MS.gene35910:intron 20.0%
!!! TTTTTGGATAAGTTGTTATA+TGG - chr5.1:55922761-55922780 None:intergenic 20.0%
! AAGAGAGAAAATTACAATGA+TGG + chr5.1:55922481-55922500 MS.gene35910:CDS 25.0%
! ACATATATGAAAGTGAAGAT+TGG - chr5.1:55922667-55922686 None:intergenic 25.0%
! TATTACTTAATTGACACCAA+CGG - chr5.1:55922962-55922981 None:intergenic 25.0%
! TGGATAATGAATACTAACAT+AGG - chr5.1:55922647-55922666 None:intergenic 25.0%
! TTATTCGATCTATTCAATTG+TGG + chr5.1:55923063-55923082 MS.gene35910:intron 25.0%
!! TTTGAATTATCAAGAGGTTT+TGG + chr5.1:55922549-55922568 MS.gene35910:CDS 25.0%
AAAACCTATTATCCAGAAAG+CGG - chr5.1:55923024-55923043 None:intergenic 30.0%
ACTTCATGTTCTTCTTTCTT+TGG - chr5.1:55922199-55922218 None:intergenic 30.0%
ATAGTAGTAATGAGCTTCAT+TGG + chr5.1:55922371-55922390 MS.gene35910:CDS 30.0%
ATTACTTAATTGACACCAAC+GGG - chr5.1:55922961-55922980 None:intergenic 30.0%
CATAAGCAAATGCAATTATG+AGG + chr5.1:55922444-55922463 MS.gene35910:CDS 30.0%
TAGTAGTAATGAGCTTCATT+GGG + chr5.1:55922372-55922391 MS.gene35910:CDS 30.0%
TGATAAAAGACCAAGAATCA+AGG + chr5.1:55923497-55923516 MS.gene35910:CDS 30.0%
! CTACTATTACAAGTGTTTTG+TGG - chr5.1:55922358-55922377 None:intergenic 30.0%
! GCAAGAATAGTCATCAATTT+TGG - chr5.1:55922875-55922894 None:intergenic 30.0%
! TAGTTGTTATATGGCCTTTT+TGG - chr5.1:55922777-55922796 None:intergenic 30.0%
!! AGTGTAAGATTTGTTGAAGT+TGG - chr5.1:55922248-55922267 None:intergenic 30.0%
!! TAAGATTTGTTGAAGTTGGA+TGG - chr5.1:55922244-55922263 None:intergenic 30.0%
!!! CAATGATGGTTTTTTGGAAA+TGG + chr5.1:55922495-55922514 MS.gene35910:CDS 30.0%
AATGGATGATGAAAAGAGTG+TGG + chr5.1:55922513-55922532 MS.gene35910:CDS 35.0%
GATAGGTAATCTTTGATGCT+TGG + chr5.1:55923116-55923135 MS.gene35910:intron 35.0%
TCATCTTTCCCTTCATCAAA+GGG - chr5.1:55922421-55922440 None:intergenic 35.0%
TCATTGGGATATCATGGAAT+GGG + chr5.1:55922387-55922406 MS.gene35910:CDS 35.0%
TTCATTGGGATATCATGGAA+TGG + chr5.1:55922386-55922405 MS.gene35910:CDS 35.0%
! AAAAGTGTGACCTTGATTCT+TGG - chr5.1:55923510-55923529 None:intergenic 35.0%
! CACATAAAGATTTTACCCGT+TGG + chr5.1:55922943-55922962 MS.gene35910:intron 35.0%
!! CAGCGTTTAATTTTCGAACT+TGG - chr5.1:55923479-55923498 None:intergenic 35.0%
ACTAACATAGGAGCCACTAT+TGG - chr5.1:55922635-55922654 None:intergenic 40.0%
AGGGATTTCCCTTTGATGAA+GGG + chr5.1:55922410-55922429 MS.gene35910:CDS 40.0%
CTCATCTTTCCCTTCATCAA+AGG - chr5.1:55922422-55922441 None:intergenic 40.0%
GAAGAGAAGCAAGTGTTCTT+AGG + chr5.1:55923406-55923425 MS.gene35910:CDS 40.0%
GAAGTTGGATGGTATTTGAG+TGG - chr5.1:55922233-55922252 None:intergenic 40.0%
GCTCTCTCTTCTTCCAATAT+TGG - chr5.1:55923384-55923403 None:intergenic 40.0%
TGAGCTTCATTGGGATATCA+TGG + chr5.1:55922381-55922400 MS.gene35910:CDS 40.0%
TTGACCGCTTTCTGGATAAT+AGG + chr5.1:55923017-55923036 MS.gene35910:intron 40.0%
! TCATTTTCCTCCTCCAATAG+TGG + chr5.1:55922619-55922638 MS.gene35910:CDS 40.0%
!!! ATCAAGAGGTTTTGGATGCA+TGG + chr5.1:55922557-55922576 MS.gene35910:CDS 40.0%
AACATAGGAGCCACTATTGG+AGG - chr5.1:55922632-55922651 None:intergenic 45.0%
AACTCTTGTTGACCGCTTTC+TGG + chr5.1:55923009-55923028 MS.gene35910:intron 45.0%
GAGGGATTTCCCTTTGATGA+AGG + chr5.1:55922409-55922428 MS.gene35910:CDS 45.0%
TGGGATATCATGGAATGGGA+GGG + chr5.1:55922391-55922410 MS.gene35910:CDS 45.0%
TTGGGATATCATGGAATGGG+AGG + chr5.1:55922390-55922409 MS.gene35910:CDS 45.0%
!! GATGGGAGAAGTGCCAATAT+TGG + chr5.1:55923368-55923387 MS.gene35910:intron 45.0%
ATAGGAGCCACTATTGGAGG+AGG - chr5.1:55922629-55922648 None:intergenic 50.0%
CATTAGCCCAAAGAGAGCCA+CGG - chr5.1:55922588-55922607 None:intergenic 50.0%
! GGTATTTGAGTGGTAGCGTC+TGG - chr5.1:55922223-55922242 None:intergenic 50.0%
GTCTGACCGTGGCTCTCTTT+GGG + chr5.1:55922579-55922598 MS.gene35910:CDS 55.0%
TTGGATGCATGGTCTGACCG+TGG + chr5.1:55922568-55922587 MS.gene35910:CDS 55.0%
GGTCTGACCGTGGCTCTCTT+TGG + chr5.1:55922578-55922597 MS.gene35910:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 55922169 55923655 55922169 ID=MS.gene35910
chr5.1 mRNA 55922169 55923655 55922169 ID=MS.gene35910.t1;Parent=MS.gene35910
chr5.1 exon 55922169 55922648 55922169 ID=MS.gene35910.t1.exon1;Parent=MS.gene35910.t1
chr5.1 CDS 55922169 55922648 55922169 ID=cds.MS.gene35910.t1;Parent=MS.gene35910.t1
chr5.1 exon 55923369 55923518 55923369 ID=MS.gene35910.t1.exon2;Parent=MS.gene35910.t1
chr5.1 CDS 55923369 55923518 55923369 ID=cds.MS.gene35910.t1;Parent=MS.gene35910.t1
chr5.1 exon 55923632 55923655 55923632 ID=MS.gene35910.t1.exon3;Parent=MS.gene35910.t1
chr5.1 CDS 55923632 55923655 55923632 ID=cds.MS.gene35910.t1;Parent=MS.gene35910.t1
Gene Sequence

>MS.gene35910

ATGAGATACACTTCATCTCTTAGAAGTCCAAAGAAAGAAGAACATGAAGTACCAGACGCTACCACTCAAATACCATCCAACTTCAACAAATCTTACACTAATGAAGATTTCTATCTTTTCGACGAACTCGAAGAGTACTTGAACATTGAAGAAGAAGAAGAAGAAGAAGAACAAAAGATATCATCACCACAAAACACTTGTAATAGTAGTAATGAGCTTCATTGGGATATCATGGAATGGGAGGGATTTCCCTTTGATGAAGGGAAAGATGAGAACATAAGCAAATGCAATTATGAGGAGAAGAAAATAATAAAGAGAGAAAATTACAATGATGGTTTTTTGGAAATGGATGATGAAAAGAGTGTGGCTTTGAATTTGAATTTGAATTATCAAGAGGTTTTGGATGCATGGTCTGACCGTGGCTCTCTTTGGGCTAATGATTGTTCTCTTTCATTTTCCTCCTCCAATAGTGGCTCCTATGTTAGTATTCATTATCCAATCTTCACTTTCATATATGTGTTTTATATTATTTTCTAATGTTTAATGCACATGTTTGCACTTCTAACTATCAATTCTTATTCTTTTAAGGCCATATAACAACTTATCCAAAAAGGCCATATAACAACTATATTATTATATTAGTCTTCCTTTTGTTACTATTTAAAATTAGCGACTATATTTGTAGAAAAAGTTGAATTATCGACCAAAATTGATGACTATTCTTGCTAATAGTGTATATTGCACACTCTTTGTTATTAATATCGAGTCGTCAAGCACATAAAGATTTTACCCGTTGGTGTCAATTAAGTAATATGTTGATTTTTGTTTAGATGGTGTGCGAACTCTTGTTGACCGCTTTCTGGATAATAGGTTTTATGTTATGACAATTTGAACTTATTCGATCTATTCAATTGTGGTTAATTTTTGTCGATAACTAAATTTTTGGTGATAGGTAATCTTTGATGCTTGGAGAACTTGATTATGTAATAATAAAGAAATAGTTAAAACTTAAGAGTGATTGATAATGTAACATATTTAGTTCATTTTGGATATAACTTGAAAGTTATTTATGTAGTTATTTTATTTGCACTCTCACTTGAAAATGAATTTGCTAGAACTAATATATTTGATTTTGAATCTCATTAACATTAGATATGAATTATTATGATATATAAACCTAGTAATTTCTTTTTTGTTTAGATGGGAGAAGTGCCAATATTGGAAGAAGAGAGAGCAAGAAGAGAAGCAAGTGTTCTTAGGTACAAAGAAAAACGTCAGAACAGATTGTTCTCAAAGAAGATAAGATACCAAGTTCGAAAATTAAACGCTGATAAAAGACCAAGAATCAAGGTCACACTTTTTGAATATTATTATTACTTTTTGTTTCTTTTTTTTTTTCATTGGGTTTTTGTAACATTTTGTTTCGTAATGAAAACATCTTTTTTGTGATATTTTTGATGTAGGGTCGCTTTGTGAAGAGACATTGA

Protein sequence

>MS.gene35910.t1

MRYTSSLRSPKKEEHEVPDATTQIPSNFNKSYTNEDFYLFDELEEYLNIEEEEEEEEQKISSPQNTCNSSNELHWDIMEWEGFPFDEGKDENISKCNYEEKKIIKRENYNDGFLEMDDEKSVALNLNLNYQEVLDAWSDRGSLWANDCSLSFSSSNSGSYMGEVPILEEERARREASVLRYKEKRQNRLFSKKIRYQVRKLNADKRPRIKGRFVKRH