AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49193


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49193.t1 MTR_5g072780 95.853 217 8 1 1 216 1 217 1.11e-146 407
MS.gene49193.t1 MTR_7g032240 39.850 133 41 3 122 215 219 351 2.26e-20 88.2
MS.gene49193.t1 MTR_1g110870 44.144 111 42 3 123 215 316 424 1.54e-19 86.3
MS.gene49193.t1 MTR_4g127420 53.488 86 36 2 128 209 293 378 2.56e-19 85.5
MS.gene49193.t1 MTR_8g104190 41.727 139 53 4 93 215 274 400 3.51e-18 82.4
MS.gene49193.t1 MTR_1g013450 36.441 118 62 3 105 215 186 297 2.19e-13 68.2
MS.gene49193.t1 MTR_3g105710 67.442 43 14 0 173 215 232 274 1.95e-11 62.4
MS.gene49193.t1 MTR_4g128930 67.442 43 14 0 173 215 298 340 4.26e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49193.t1 AT1G07050 67.000 100 26 3 118 215 99 193 2.36e-37 129
MS.gene49193.t1 AT1G07050 64.894 94 26 3 118 209 99 187 2.42e-32 116
MS.gene49193.t1 AT5G14370 53.211 109 36 3 122 215 228 336 2.53e-28 109
MS.gene49193.t1 AT4G25990 53.922 102 39 3 118 215 286 383 1.07e-23 97.8
MS.gene49193.t1 AT5G57180 53.846 91 40 2 125 215 337 425 5.68e-23 95.9
MS.gene49193.t1 AT1G73870 42.553 141 57 5 93 215 253 387 5.07e-21 90.1
MS.gene49193.t1 AT4G25990 47.009 117 39 4 118 215 286 398 6.10e-20 87.4
MS.gene49193.t1 AT4G25990 52.083 96 38 3 118 209 286 377 1.42e-19 86.3
MS.gene49193.t1 AT1G49130 52.747 91 34 3 127 215 237 320 2.02e-19 85.1
MS.gene49193.t1 AT1G49130 52.747 91 34 3 127 215 230 313 2.15e-19 85.1
MS.gene49193.t1 AT1G25440 46.429 112 42 4 122 215 292 403 4.27e-19 85.1
MS.gene49193.t1 AT5G57180 51.765 85 39 2 125 209 337 419 8.65e-19 84.3
MS.gene49193.t1 AT1G68520 41.525 118 51 3 116 215 282 399 1.10e-18 84.0
MS.gene49193.t1 AT2G24790 61.111 54 21 0 162 215 218 271 3.77e-13 67.4
MS.gene49193.t1 AT5G24930 53.226 62 29 0 154 215 276 337 5.54e-12 64.3
MS.gene49193.t1 AT5G57660 69.767 43 13 0 173 215 285 327 1.22e-11 63.5

Find 33 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCATGTTCTTCTTTCTT+TGG 0.183818 5.4:+61429624 None:intergenic
CTCATAATTGCATTTGCTTA+TGG 0.208005 5.4:+61429380 None:intergenic
GGTCTGACCGTGGCTCTCTT+TGG 0.244079 5.4:-61429245 MS.gene49193:CDS
GCTCTCTCTTCTTCCAATAT+TGG 0.250193 5.4:+61428442 None:intergenic
TTTGAATTATCAAGAGGTTT+TGG 0.259887 5.4:-61429274 MS.gene49193:CDS
AAAAGTGTGACCTTGATTCT+TGG 0.281388 5.4:+61428316 None:intergenic
GAAGAGAAGCAAGTGTTCTT+AGG 0.336563 5.4:-61428417 MS.gene49193:CDS
GTCTGACCGTGGCTCTCTTT+GGG 0.337400 5.4:-61429244 MS.gene49193:CDS
GATGGGAGAAGTGCCAATAT+TGG 0.355037 5.4:-61428455 MS.gene49193:CDS
AGTGTAAGATTTGTTGAAGT+TGG 0.374346 5.4:+61429575 None:intergenic
GGTATTTGAGTGGTAGCTTC+TGG 0.391867 5.4:+61429600 None:intergenic
ATAGTAGTAATGAGCTTCAT+TGG 0.401933 5.4:-61429452 MS.gene49193:CDS
AAGAGAGAAAATTACAATGA+TGG 0.429279 5.4:-61429342 MS.gene49193:CDS
TGAGCTTCATTGGGATATCA+TGG 0.472007 5.4:-61429442 MS.gene49193:CDS
CATAAGCAAATGCAATTATG+AGG 0.478747 5.4:-61429379 MS.gene49193:CDS
GAGGGATTTCCCTTTGATGA+AGG 0.484035 5.4:-61429414 MS.gene49193:CDS
ACTAACATAGGAGCCACTAT+TGG 0.497136 5.4:+61429191 None:intergenic
TAGTAGTAATGAGCTTCATT+GGG 0.508412 5.4:-61429451 MS.gene49193:CDS
AGGGATTTCCCTTTGATGAA+GGG 0.520507 5.4:-61429413 MS.gene49193:CDS
TCATTGGGATATCATGGAAT+GGG 0.534580 5.4:-61429436 MS.gene49193:CDS
TTCATTGGGATATCATGGAA+TGG 0.539426 5.4:-61429437 MS.gene49193:CDS
TTTGAATTTGAATTATCAAG+AGG 0.541289 5.4:-61429280 MS.gene49193:CDS
AACATAGGAGCCACTATTGG+AGG 0.541652 5.4:+61429194 None:intergenic
CGCTTTGTGAAGAGACATTG+AGG 0.551371 5.4:-61428186 None:intergenic
TAAGATTTGTTGAAGTTGGA+TGG 0.551571 5.4:+61429579 None:intergenic
TGATAAAAGACCAAGAATCA+AGG 0.578251 5.4:-61428326 MS.gene49193:intron
ATAGGAGCCACTATTGGAGG+AGG 0.606351 5.4:+61429197 None:intergenic
TTGGGATATCATGGAATGGG+AGG 0.609318 5.4:-61429433 MS.gene49193:CDS
GAAGTTGGATGGTATTTGAG+TGG 0.615620 5.4:+61429590 None:intergenic
TGGGATATCATGGAATGGGA+GGG 0.659236 5.4:-61429432 MS.gene49193:CDS
CATTAGCCCAAAGAGAGCCA+CGG 0.677020 5.4:+61429238 None:intergenic
AGTGGATGATGAAAAGAGTG+TGG 0.691548 5.4:-61429310 MS.gene49193:CDS
TTGGATGCATGGTCTGACCG+TGG 0.694015 5.4:-61429255 MS.gene49193:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATAGTTGTTATA+TGG + chr5.4:61428802-61428821 None:intergenic 10.0%
!!! TAATTTTAAATAGTAACAAA+AGG + chr5.4:61428834-61428853 None:intergenic 10.0%
!!! TTCTTTTTTTTTTTTTCATT+GGG - chr5.4:61429569-61429588 MS.gene49193:CDS 10.0%
!!! TTTCTTTTTTTTTTTTTCAT+TGG - chr5.4:61429568-61429587 MS.gene49193:CDS 10.0%
!! TAAACAAAAAAGAAATTACT+AGG + chr5.4:61429364-61429383 None:intergenic 15.0%
!!! AATTTCTTTTTTGTTTAGAT+GGG - chr5.4:61429367-61429386 MS.gene49193:CDS 15.0%
!!! TAATTTCTTTTTTGTTTAGA+TGG - chr5.4:61429366-61429385 MS.gene49193:CDS 15.0%
!!! TATCAATTCTTATTCTTTTA+AGG - chr5.4:61428751-61428770 MS.gene49193:intron 15.0%
!! ATATAACAACTTATCCAAAA+AGG - chr5.4:61428776-61428795 MS.gene49193:intron 20.0%
!! TTTGAATTTGAATTATCAAG+AGG - chr5.4:61428559-61428578 MS.gene49193:intron 20.0%
!!! AAATTACAATGATGGTTTTT+TGG - chr5.4:61428505-61428524 MS.gene49193:intron 20.0%
!!! ATAACTAAATTTTTGGTGAT+AGG - chr5.4:61429115-61429134 MS.gene49193:intron 20.0%
!!! GTAACATATTTAGTTCATTT+TGG - chr5.4:61429211-61429230 MS.gene49193:CDS 20.0%
!!! TTTGTCGATAACTAAATTTT+TGG - chr5.4:61429108-61429127 MS.gene49193:intron 20.0%
!!! TTTGTGATATTTTTGATGTA+GGG - chr5.4:61429629-61429648 MS.gene49193:CDS 20.0%
!!! TTTTGTGATATTTTTGATGT+AGG - chr5.4:61429628-61429647 MS.gene49193:CDS 20.0%
!!! TTTTTGGATAAGTTGTTATA+TGG + chr5.4:61428777-61428796 None:intergenic 20.0%
! AAGAGAGAAAATTACAATGA+TGG - chr5.4:61428497-61428516 MS.gene49193:intron 25.0%
! ACATATATGAAAGTGAAGAT+TGG + chr5.4:61428683-61428702 None:intergenic 25.0%
! TATTACTTAATTGACACCAA+CGG + chr5.4:61428978-61428997 None:intergenic 25.0%
! TGGATAATGAATACTAACAT+AGG + chr5.4:61428663-61428682 None:intergenic 25.0%
! TTATTCGATCTATTCAATTG+TGG - chr5.4:61429079-61429098 MS.gene49193:intron 25.0%
!! TTTGAATTATCAAGAGGTTT+TGG - chr5.4:61428565-61428584 MS.gene49193:intron 25.0%
!!! TATGTTGACTTTTGTTTAGA+TGG - chr5.4:61428996-61429015 MS.gene49193:intron 25.0%
AAAACCTATTATCCAGAAAG+CGG + chr5.4:61429040-61429059 None:intergenic 30.0%
ACTTCATGTTCTTCTTTCTT+TGG + chr5.4:61428218-61428237 None:intergenic 30.0%
AGAAGAAGAAGAAGAACAAA+AGG - chr5.4:61428340-61428359 MS.gene49193:CDS 30.0%
ATAGTAGTAATGAGCTTCAT+TGG - chr5.4:61428387-61428406 MS.gene49193:CDS 30.0%
ATTACTTAATTGACACCAAC+GGG + chr5.4:61428977-61428996 None:intergenic 30.0%
CATAAGCAAATGCAATTATG+AGG - chr5.4:61428460-61428479 MS.gene49193:intron 30.0%
CTCATAATTGCATTTGCTTA+TGG + chr5.4:61428462-61428481 None:intergenic 30.0%
TAGTAGTAATGAGCTTCATT+GGG - chr5.4:61428388-61428407 MS.gene49193:CDS 30.0%
TGATAAAAGACCAAGAATCA+AGG - chr5.4:61429513-61429532 MS.gene49193:CDS 30.0%
! CTACTATTACAAGTGTTTTG+TGG + chr5.4:61428374-61428393 None:intergenic 30.0%
! GCAAGAATAGTCATCAATTT+TGG + chr5.4:61428891-61428910 None:intergenic 30.0%
! TAGTTGTTATATGGCCTTTT+TGG + chr5.4:61428793-61428812 None:intergenic 30.0%
!! AGTGTAAGATTTGTTGAAGT+TGG + chr5.4:61428267-61428286 None:intergenic 30.0%
!! TAAGATTTGTTGAAGTTGGA+TGG + chr5.4:61428263-61428282 None:intergenic 30.0%
!! TCATTTTTCCCTTCATCAAA+GGG + chr5.4:61428437-61428456 None:intergenic 30.0%
GATAGGTAATCTTTGATGCT+TGG - chr5.4:61429132-61429151 MS.gene49193:intron 35.0%
TCATTGGGATATCATGGAAT+GGG - chr5.4:61428403-61428422 MS.gene49193:CDS 35.0%
TTCATTGGGATATCATGGAA+TGG - chr5.4:61428402-61428421 MS.gene49193:CDS 35.0%
! AAAAGTGTGACCTTGATTCT+TGG + chr5.4:61429526-61429545 None:intergenic 35.0%
! CACATATAGATTTTACCCGT+TGG - chr5.4:61428959-61428978 MS.gene49193:intron 35.0%
!! CAGCGTTTAATTTTCGAACT+TGG + chr5.4:61429495-61429514 None:intergenic 35.0%
!! CTCATTTTTCCCTTCATCAA+AGG + chr5.4:61428438-61428457 None:intergenic 35.0%
!!! CAATGATGGTTTTTTGGAAG+TGG - chr5.4:61428511-61428530 MS.gene49193:intron 35.0%
ACTAACATAGGAGCCACTAT+TGG + chr5.4:61428651-61428670 None:intergenic 40.0%
AGGGATTTCCCTTTGATGAA+GGG - chr5.4:61428426-61428445 MS.gene49193:CDS 40.0%
AGTGGATGATGAAAAGAGTG+TGG - chr5.4:61428529-61428548 MS.gene49193:intron 40.0%
GAAGAGAAGCAAGTGTTCTT+AGG - chr5.4:61429422-61429441 MS.gene49193:CDS 40.0%
GAAGTTGGATGGTATTTGAG+TGG + chr5.4:61428252-61428271 None:intergenic 40.0%
GCTCTCTCTTCTTCCAATAT+TGG + chr5.4:61429400-61429419 None:intergenic 40.0%
TGAGCTTCATTGGGATATCA+TGG - chr5.4:61428397-61428416 MS.gene49193:CDS 40.0%
TTGACCGCTTTCTGGATAAT+AGG - chr5.4:61429033-61429052 MS.gene49193:intron 40.0%
! TCATTTTCCTCCTCCAATAG+TGG - chr5.4:61428635-61428654 MS.gene49193:intron 40.0%
!!! ATCAAGAGGTTTTGGATGCA+TGG - chr5.4:61428573-61428592 MS.gene49193:intron 40.0%
AACATAGGAGCCACTATTGG+AGG + chr5.4:61428648-61428667 None:intergenic 45.0%
AACTCTTGTTGACCGCTTTC+TGG - chr5.4:61429025-61429044 MS.gene49193:intron 45.0%
GAGGGATTTCCCTTTGATGA+AGG - chr5.4:61428425-61428444 MS.gene49193:CDS 45.0%
GGTATTTGAGTGGTAGCTTC+TGG + chr5.4:61428242-61428261 None:intergenic 45.0%
TGGGATATCATGGAATGGGA+GGG - chr5.4:61428407-61428426 MS.gene49193:CDS 45.0%
TTGGGATATCATGGAATGGG+AGG - chr5.4:61428406-61428425 MS.gene49193:CDS 45.0%
!! GATGGGAGAAGTGCCAATAT+TGG - chr5.4:61429384-61429403 MS.gene49193:CDS 45.0%
ATAGGAGCCACTATTGGAGG+AGG + chr5.4:61428645-61428664 None:intergenic 50.0%
CATTAGCCCAAAGAGAGCCA+CGG + chr5.4:61428604-61428623 None:intergenic 50.0%
GTCTGACCGTGGCTCTCTTT+GGG - chr5.4:61428595-61428614 MS.gene49193:intron 55.0%
TTGGATGCATGGTCTGACCG+TGG - chr5.4:61428584-61428603 MS.gene49193:intron 55.0%
GGTCTGACCGTGGCTCTCTT+TGG - chr5.4:61428594-61428613 MS.gene49193:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 61428188 61429673 61428188 ID=MS.gene49193
chr5.4 mRNA 61428188 61429673 61428188 ID=MS.gene49193.t1;Parent=MS.gene49193
chr5.4 exon 61429197 61429673 61429197 ID=MS.gene49193.t1.exon1;Parent=MS.gene49193.t1
chr5.4 CDS 61429197 61429673 61429197 ID=cds.MS.gene49193.t1;Parent=MS.gene49193.t1
chr5.4 exon 61428327 61428476 61428327 ID=MS.gene49193.t1.exon2;Parent=MS.gene49193.t1
chr5.4 CDS 61428327 61428476 61428327 ID=cds.MS.gene49193.t1;Parent=MS.gene49193.t1
chr5.4 exon 61428188 61428211 61428188 ID=MS.gene49193.t1.exon3;Parent=MS.gene49193.t1
chr5.4 CDS 61428188 61428211 61428188 ID=cds.MS.gene49193.t1;Parent=MS.gene49193.t1
Gene Sequence

>MS.gene49193

ATGAGATACACTTCATCTCTTAGAAGTCCAAAGAAAGAAGAACATGAAGTACCAGAAGCTACCACTCAAATACCATCCAACTTCAACAAATCTTACACTAATGAAGATTTCTATCTTTTCGACGAACTCGAAGAGTACTTGAACATTGAAGAAGAAGAAGAAGAAGAACAAAAGGTATCATCACCACAAAACACTTGTAATAGTAGTAATGAGCTTCATTGGGATATCATGGAATGGGAGGGATTTCCCTTTGATGAAGGGAAAAATGAGACCATAAGCAAATGCAATTATGAGGAGAAGAAAATAATAAAGAGAGAAAATTACAATGATGGTTTTTTGGAAGTGGATGATGAAAAGAGTGTGGCTTTGAATTTGAATTTGAATTATCAAGAGGTTTTGGATGCATGGTCTGACCGTGGCTCTCTTTGGGCTAATGATTGTTCTCTTTCATTTTCCTCCTCCAATAGTGGCTCCTATGTTAGTATTCATTATCCAATCTTCACTTTCATATATGTGTTTTATATTATTTTCTAATGTTTAATGCACATGTTTGCACTTCTAACTATCAATTCTTATTCTTTTAAGGCCATATAACAACTTATCCAAAAAGGCCATATAACAACTATATTATTATATTAGTCTTCCTTTTGTTACTATTTAAAATTAGCGACTATATTTGTAGAAAAAGTTGAATTATCGACCAAAATTGATGACTATTCTTGCTAATAGTGTATATTGCACACTCTTTGTTATTAATATCGAGTCGTCAAGCACATATAGATTTTACCCGTTGGTGTCAATTAAGTAATATGTTGACTTTTGTTTAGATGGTGTGCGAACTCTTGTTGACCGCTTTCTGGATAATAGGTTTTATGTTATGACAATTTGAACTTATTCGATCTATTCAATTGTGGTTAATTTTTGTCGATAACTAAATTTTTGGTGATAGGTAATCTTTGATGCTTGGAGAACTTGATTATGTAATAATAAAGAAATAGTTAAGACTTAAGAGTGATTGATAATGTAACATATTTAGTTCATTTTGGATATAACTTGAAAGTTATTTATGTAGTTATTTTATTTGCACTCTCACTTGAAAATGAATTTGCTAGAACTAATATATTTGATTTTGAATCTCATTAACATTAGATATGAATTATTATGATATATAAACCTAGTAATTTCTTTTTTGTTTAGATGGGAGAAGTGCCAATATTGGAAGAAGAGAGAGCAAGAAGAGAAGCAAGTGTTCTTAGGTACAAAGAAAAACGTCAGAACAGATTGTTCTCAAAGAAGATAAGATACCAAGTTCGAAAATTAAACGCTGATAAAAGACCAAGAATCAAGGTCACACTTTTTGAATATTATTATTACTTTTTGTTTCTTTTTTTTTTTTTCATTGGGTTTTTGTAACATTTTGTTTCGTAATGAAAACATCTTTTTTGTGATATTTTTGATGTAGGGTCGCTTTGTGAAGAGACATTGA

Protein sequence

>MS.gene49193.t1

MRYTSSLRSPKKEEHEVPEATTQIPSNFNKSYTNEDFYLFDELEEYLNIEEEEEEEQKVSSPQNTCNSSNELHWDIMEWEGFPFDEGKNETISKCNYEEKKIIKRENYNDGFLEVDDEKSVALNLNLNYQEVLDAWSDRGSLWANDCSLSFSSSNSGSYMGEVPILEEERARREASVLRYKEKRQNRLFSKKIRYQVRKLNADKRPRIKGRFVKRH