Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058794.t1 | XP_003598062.2 | 98 | 606 | 12 | 0 | 1 | 606 | 101 | 706 | 0.00E+00 | 1173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058794.t1 | Q9ZRV4 | 67.8 | 606 | 191 | 1 | 1 | 606 | 123 | 724 | 8.8e-238 | 824.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058794.t1 | G7J070 | 98.0 | 606 | 12 | 0 | 1 | 606 | 101 | 706 | 0.0e+00 | 1173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058794 | MS.gene058795 | 0.843425 | 1.41E-58 | -1.69E-46 |
| MS.gene058794 | MS.gene52850 | 0.974501 | 2.88E-138 | -1.69E-46 |
| MS.gene058794 | MS.gene96593 | 0.853282 | 2.65E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 121 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGAGTTTGTGCTTAGTTT+AGG | 0.245169 | 3.3:-1482048 | MS.gene058794:CDS |
| CTGAGTTTGTGCTTAGTTTA+GGG | 0.247718 | 3.3:-1482047 | MS.gene058794:CDS |
| GTTCTAACCAACTTATTTAC+TGG | 0.258224 | 3.3:+1485451 | None:intergenic |
| TTTCAAACCACCTCCTTCTA+TGG | 0.261404 | 3.3:-1480094 | MS.gene058794:CDS |
| ACAATTGAAATATATATAAA+AGG | 0.283295 | 3.3:+1487078 | None:intergenic |
| GGTATGCTGGAGGAGTATTT+TGG | 0.292415 | 3.3:-1482150 | MS.gene058794:CDS |
| GATGCCATCAGAACAGTTTA+TGG | 0.295437 | 3.3:-1487802 | MS.gene058794:CDS |
| CGTGAAAGACTTGATTATAT+TGG | 0.303621 | 3.3:-1483192 | MS.gene058794:CDS |
| AAGGCCAATTGCATGGTTAC+AGG | 0.305312 | 3.3:-1490283 | MS.gene058794:intron |
| AGGCCCTGTGCTGCTGTAAA+AGG | 0.310412 | 3.3:-1488638 | MS.gene058794:CDS |
| ATAAAAGGCTTAGATGCTTT+AGG | 0.329580 | 3.3:+1487093 | None:intergenic |
| TGAGAATCTATTCTATAATA+TGG | 0.337500 | 3.3:-1488112 | MS.gene058794:CDS |
| TGGCATTCATATTGATGATC+AGG | 0.346186 | 3.3:-1482130 | MS.gene058794:CDS |
| TCAAACAGTTTCAGCTGCTC+TGG | 0.349043 | 3.3:-1481631 | MS.gene058794:CDS |
| GTTCGTACCTCTCTCTTTGT+TGG | 0.351550 | 3.3:+1487021 | None:intergenic |
| CAGCAAGTTCTCAGCCGTTT+TGG | 0.360552 | 3.3:-1483251 | MS.gene058794:CDS |
| TGTAAAAGGAACACAGATAA+TGG | 0.367128 | 3.3:-1488624 | MS.gene058794:intron |
| TTGAAATGTACGTGCATCAT+TGG | 0.368233 | 3.3:+1486422 | None:intergenic |
| TGATAACTTCCTGATTTAAG+AGG | 0.384335 | 3.3:+1486487 | None:intergenic |
| TGAGTTCTATAGAATCTTGT+AGG | 0.400669 | 3.3:+1484324 | None:intergenic |
| CCTCCTTCTATGGCTACTAA+TGG | 0.417391 | 3.3:-1480084 | MS.gene058794:CDS |
| ATGCCATCAGAACAGTTTAT+GGG | 0.417980 | 3.3:-1487801 | MS.gene058794:CDS |
| CTAACCTGTAACCATGCAAT+TGG | 0.429908 | 3.3:+1490279 | None:intergenic |
| CTCCATGACACCATCTCTAT+AGG | 0.431084 | 3.3:+1488670 | None:intergenic |
| ATAGGTGAGCCTCTTAAATC+AGG | 0.431743 | 3.3:-1486496 | MS.gene058794:intron |
| TACTAATGGAACCTTTGTTA+AGG | 0.435371 | 3.3:-1480070 | MS.gene058794:CDS |
| CAATCCCTCACCAGATGGAT+TGG | 0.436586 | 3.3:+1481575 | None:intergenic |
| TTTATGTTTCTCAGATAGAC+TGG | 0.439450 | 3.3:-1487169 | MS.gene058794:intron |
| TTTACAGCAGCACAGGGCCT+AGG | 0.449578 | 3.3:+1488641 | None:intergenic |
| GAAAACAAAGGCTGGTATGC+TGG | 0.453883 | 3.3:-1482163 | MS.gene058794:CDS |
| GAAGACACTACAAAATTCTT+CGG | 0.456465 | 3.3:-1488079 | MS.gene058794:CDS |
| GTTCCATTAGTAGCCATAGA+AGG | 0.456923 | 3.3:+1480081 | None:intergenic |
| TTGCATGGGCCCAGCGTGAA+TGG | 0.464113 | 3.3:-1480155 | MS.gene058794:CDS |
| CATCTATCCTGTAGGAATGA+TGG | 0.469466 | 3.3:-1483608 | MS.gene058794:intron |
| TTTCTGAAGCTGAGATTGCA+TGG | 0.470561 | 3.3:-1480170 | MS.gene058794:CDS |
| TTAGCTGAATGGCATTGAAA+TGG | 0.472900 | 3.3:+1483228 | None:intergenic |
| CCGATGTTGCCCAATCCATC+TGG | 0.474634 | 3.3:-1481585 | MS.gene058794:CDS |
| TTCCCATACCCGGTGGGGTA+GGG | 0.475091 | 3.3:+1485581 | None:intergenic |
| GGCATTCATATTGATGATCA+GGG | 0.480584 | 3.3:-1482129 | MS.gene058794:CDS |
| AATATAATCAAGTCTTTCAC+GGG | 0.482818 | 3.3:+1483194 | None:intergenic |
| TCAGGGATGCGATCCATGTC+CGG | 0.485690 | 3.3:+1482066 | None:intergenic |
| TACCCGGTGGGGTAGGGCTG+AGG | 0.486411 | 3.3:+1485587 | None:intergenic |
| TTCTGAAGCTGAGATTGCAT+GGG | 0.487761 | 3.3:-1480169 | MS.gene058794:CDS |
| CCTAAACTAAGCACAAACTC+AGG | 0.490379 | 3.3:+1482048 | None:intergenic |
| CTTGATTATATTGGCACTGA+AGG | 0.492658 | 3.3:-1483183 | MS.gene058794:CDS |
| TCATACTTGTGGCAGACAGC+TGG | 0.499733 | 3.3:-1485652 | MS.gene058794:intron |
| TGGCATTGAAATGGCCAAAA+CGG | 0.505388 | 3.3:+1483237 | None:intergenic |
| GAGAATGCCTGTGATAATAC+TGG | 0.506862 | 3.3:-1481510 | MS.gene058794:intron |
| TAGAGATGGTGTCATGGAGC+AGG | 0.507145 | 3.3:-1488666 | MS.gene058794:CDS |
| CAATATAATCAAGTCTTTCA+CGG | 0.507914 | 3.3:+1483193 | None:intergenic |
| CAAACAGTTTCAGCTGCTCT+GGG | 0.509172 | 3.3:-1481630 | MS.gene058794:CDS |
| TGTCAATTTCTCTTGCAGAA+AGG | 0.511158 | 3.3:-1488001 | MS.gene058794:intron |
| GGCTGGATCACTTAGCTGAA+TGG | 0.514732 | 3.3:+1483217 | None:intergenic |
| ACTGTTACTACTATTACTAA+AGG | 0.515263 | 3.3:-1490302 | MS.gene058794:CDS |
| AATCCCTCACCAGATGGATT+GGG | 0.516217 | 3.3:+1481576 | None:intergenic |
| ATTGACATTGGTATGATGTA+TGG | 0.519238 | 3.3:+1488017 | None:intergenic |
| GGATGATTACTCAAAGATAG+TGG | 0.524793 | 3.3:-1488058 | MS.gene058794:CDS |
| GAAAGTGCCAGTAAATAAGT+TGG | 0.524800 | 3.3:-1485458 | MS.gene058794:CDS |
| TTGCAAATGTTGTAAACTTG+AGG | 0.530370 | 3.3:-1483507 | MS.gene058794:intron |
| TTTCCCATACCCGGTGGGGT+AGG | 0.533882 | 3.3:+1485580 | None:intergenic |
| GATCCATGTCCGGAGTATAC+TGG | 0.534406 | 3.3:+1482076 | None:intergenic |
| ATCAATAACAACTATGACCC+TGG | 0.540120 | 3.3:-1484275 | MS.gene058794:intron |
| TAACCTCAGCCCTACCCCAC+CGG | 0.548727 | 3.3:-1485590 | MS.gene058794:CDS |
| TTGTATTGACCATTCACGCT+GGG | 0.551512 | 3.3:+1480146 | None:intergenic |
| TATATTGGCACTGAAGGAAG+AGG | 0.554059 | 3.3:-1483177 | MS.gene058794:CDS |
| TAGGCGTGTAATCTGCCTGC+AGG | 0.557560 | 3.3:+1485415 | None:intergenic |
| ACAGATTCATTAGATCCTGC+AGG | 0.558475 | 3.3:-1485430 | MS.gene058794:CDS |
| TATCATTGAGAAGATACAAT+CGG | 0.558745 | 3.3:-1486469 | MS.gene058794:CDS |
| CGCAAACATTACCTTAACAA+AGG | 0.567048 | 3.3:+1480059 | None:intergenic |
| GATCTGCTGAAAACAAAGGC+TGG | 0.567731 | 3.3:-1482171 | MS.gene058794:CDS |
| ACATTGACATCAATATTCTC+AGG | 0.568147 | 3.3:+1487048 | None:intergenic |
| AAAAGCAATCCCTCACCAGA+TGG | 0.569149 | 3.3:+1481570 | None:intergenic |
| CCAGATGGATTGGGCAACAT+CGG | 0.571394 | 3.3:+1481585 | None:intergenic |
| GTAAAGCACTGCACATATGT+TGG | 0.572709 | 3.3:-1483580 | MS.gene058794:CDS |
| TCTGCAAGAGAAATTGACAT+TGG | 0.573051 | 3.3:+1488005 | None:intergenic |
| GACCCCATAAACTGTTCTGA+TGG | 0.573196 | 3.3:+1487798 | None:intergenic |
| ATTACTAAAGGCCAATTGCA+TGG | 0.575626 | 3.3:-1490290 | MS.gene058794:CDS |
| GTCCATACCAGTATTATCAC+AGG | 0.577956 | 3.3:+1481503 | None:intergenic |
| TCTCAGATAGACTGGTTGAG+TGG | 0.579246 | 3.3:-1487161 | MS.gene058794:intron |
| GTATACTGGTCAAGTATCAC+AGG | 0.580775 | 3.3:+1482090 | None:intergenic |
| AAACATTTCCCATACCCGGT+GGG | 0.580830 | 3.3:+1485575 | None:intergenic |
| AACCTCAGCCCTACCCCACC+GGG | 0.583112 | 3.3:-1485589 | MS.gene058794:intron |
| GTGTCACTTTGAATGCAGTA+AGG | 0.584850 | 3.3:-1485357 | MS.gene058794:intron |
| GGCATCAAGCCTTGATGATG+TGG | 0.587629 | 3.3:+1487819 | None:intergenic |
| CGGTGATTGAATCAACATTG+AGG | 0.588119 | 3.3:-1486449 | MS.gene058794:CDS |
| GATGGATTGGGCAACATCGG+TGG | 0.588877 | 3.3:+1481588 | None:intergenic |
| CTCTCGTACTAATAAGAGCA+AGG | 0.589552 | 3.3:-1485617 | MS.gene058794:CDS |
| GCACTGCACATATGTTGGAA+TGG | 0.589828 | 3.3:-1483575 | MS.gene058794:CDS |
| AACATTTCCCATACCCGGTG+GGG | 0.591924 | 3.3:+1485576 | None:intergenic |
| GCTTGCTAGTATGACTTATG+TGG | 0.595892 | 3.3:-1490333 | MS.gene058794:CDS |
| ATTATCACAGGCATTCTCCA+TGG | 0.601535 | 3.3:+1481515 | None:intergenic |
| GTGTCATGGAGCAGGAACCT+AGG | 0.602402 | 3.3:-1488658 | MS.gene058794:CDS |
| TTAGTAGCCATAGAAGGAGG+TGG | 0.606651 | 3.3:+1480087 | None:intergenic |
| CTAAACTAAGCACAAACTCA+GGG | 0.607686 | 3.3:+1482049 | None:intergenic |
| ATCCTATAGAGATGGTGTCA+TGG | 0.613728 | 3.3:-1488672 | MS.gene058794:CDS |
| TTGCCCAATCCATCTGGTGA+GGG | 0.615762 | 3.3:-1481579 | MS.gene058794:CDS |
| AGGTTGATTGGGATGATGAA+AGG | 0.616203 | 3.3:-1481662 | MS.gene058794:CDS |
| ATACAGTAGCCACATCATCA+AGG | 0.617880 | 3.3:-1487828 | MS.gene058794:CDS |
| GCCTACATGCAAGTTATACC+TGG | 0.618767 | 3.3:-1485397 | MS.gene058794:CDS |
| TTTAGGGTATCCTATAGAGA+TGG | 0.620969 | 3.3:-1488680 | MS.gene058794:intron |
| GTTGCCCAATCCATCTGGTG+AGG | 0.621707 | 3.3:-1481580 | MS.gene058794:CDS |
| CTTGCTCTTATTAGTACGAG+AGG | 0.624813 | 3.3:+1485618 | None:intergenic |
| TATTCTATAATATGGCTGCA+AGG | 0.627616 | 3.3:-1488104 | MS.gene058794:CDS |
| GTTGTATTGACCATTCACGC+TGG | 0.629559 | 3.3:+1480145 | None:intergenic |
| TGACCAGTATACTCCGGACA+TGG | 0.629857 | 3.3:-1482079 | MS.gene058794:CDS |
| AGATAGTGGATGTTGTCAGT+CGG | 0.631946 | 3.3:-1488044 | MS.gene058794:CDS |
| CACAGATCTGCTGAAAACAA+AGG | 0.634034 | 3.3:-1482175 | MS.gene058794:CDS |
| TGCCATCAGAACAGTTTATG+GGG | 0.642839 | 3.3:-1487800 | MS.gene058794:CDS |
| TACATGCAAGTTATACCTGG+TGG | 0.650299 | 3.3:-1485394 | MS.gene058794:CDS |
| ACCAGGTATAACTTGCATGT+AGG | 0.658521 | 3.3:+1485396 | None:intergenic |
| GCTCCTTCGTTGTCTAACTG+AGG | 0.662697 | 3.3:+1484346 | None:intergenic |
| AACAAAGGCTGGTATGCTGG+AGG | 0.667613 | 3.3:-1482160 | MS.gene058794:CDS |
| CCATTAGTAGCCATAGAAGG+AGG | 0.673543 | 3.3:+1480084 | None:intergenic |
| GATACTTGACCAGTATACTC+CGG | 0.679952 | 3.3:-1482085 | MS.gene058794:CDS |
| TGTGCATCCAACAAAGAGAG+AGG | 0.681638 | 3.3:-1487028 | MS.gene058794:intron |
| TTGACATCAATATTCTCAGG+AGG | 0.686791 | 3.3:+1487051 | None:intergenic |
| ATATAATCAAGTCTTTCACG+GGG | 0.688244 | 3.3:+1483195 | None:intergenic |
| GATCCTCAGTTAGACAACGA+AGG | 0.703074 | 3.3:-1484349 | MS.gene058794:CDS |
| TGCTGCCAAGAAAATCACGA+TGG | 0.717164 | 3.3:-1487680 | MS.gene058794:CDS |
| TCGTACTAATAAGAGCAAGG+AGG | 0.754469 | 3.3:-1485614 | MS.gene058794:CDS |
| TATAATCAAGTCTTTCACGG+GGG | 0.793097 | 3.3:+1483196 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 1480056 | 1490362 | 1480056 | ID=MS.gene058794 |
| chr3.3 | mRNA | 1480056 | 1490362 | 1480056 | ID=MS.gene058794.t1;Parent=MS.gene058794 |
| chr3.3 | exon | 1490284 | 1490362 | 1490284 | ID=MS.gene058794.t1.exon1;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1490284 | 1490362 | 1490284 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1488625 | 1488697 | 1488625 | ID=MS.gene058794.t1.exon2;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1488625 | 1488697 | 1488625 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1488002 | 1488136 | 1488002 | ID=MS.gene058794.t1.exon3;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1488002 | 1488136 | 1488002 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1487665 | 1487875 | 1487665 | ID=MS.gene058794.t1.exon4;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1487665 | 1487875 | 1487665 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1487029 | 1487177 | 1487029 | ID=MS.gene058794.t1.exon5;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1487029 | 1487177 | 1487029 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1486416 | 1486514 | 1486416 | ID=MS.gene058794.t1.exon6;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1486416 | 1486514 | 1486416 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1485590 | 1485659 | 1485590 | ID=MS.gene058794.t1.exon7;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1485590 | 1485659 | 1485590 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1485358 | 1485493 | 1485358 | ID=MS.gene058794.t1.exon8;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1485358 | 1485493 | 1485358 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1484276 | 1484370 | 1484276 | ID=MS.gene058794.t1.exon9;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1484276 | 1484370 | 1484276 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1483508 | 1483616 | 1483508 | ID=MS.gene058794.t1.exon10;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1483508 | 1483616 | 1483508 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1483124 | 1483289 | 1483124 | ID=MS.gene058794.t1.exon11;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1483124 | 1483289 | 1483124 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1482041 | 1482202 | 1482041 | ID=MS.gene058794.t1.exon12;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1482041 | 1482202 | 1482041 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1481511 | 1481682 | 1481511 | ID=MS.gene058794.t1.exon13;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1481511 | 1481682 | 1481511 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| chr3.3 | exon | 1480056 | 1480231 | 1480056 | ID=MS.gene058794.t1.exon14;Parent=MS.gene058794.t1 |
| chr3.3 | CDS | 1480056 | 1480231 | 1480056 | ID=cds.MS.gene058794.t1;Parent=MS.gene058794.t1 |
| Gene Sequence |
| Protein sequence |
