Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52850.t1 | XP_003598062.2 | 97.9 | 573 | 12 | 0 | 1 | 573 | 134 | 706 | 0.00E+00 | 1109.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52850.t1 | Q9ZRV4 | 66.1 | 573 | 190 | 1 | 1 | 573 | 156 | 724 | 1.1e-218 | 760.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52850.t1 | G7J070 | 97.9 | 573 | 12 | 0 | 1 | 573 | 134 | 706 | 0.0e+00 | 1109.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058794 | MS.gene52850 | 0.974501 | 2.88E-138 | -1.69E-46 |
| MS.gene058795 | MS.gene52850 | 0.856941 | 2.29E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044567 | MS.gene52850 | PPI |
| MS.gene04418 | MS.gene52850 | PPI |
| MS.gene20720 | MS.gene52850 | PPI |
| MS.gene050408 | MS.gene52850 | PPI |
| MS.gene86545 | MS.gene52850 | PPI |
| MS.gene05640 | MS.gene52850 | PPI |
| MS.gene52850 | MS.gene96949 | PPI |
| MS.gene049266 | MS.gene52850 | PPI |
| MS.gene07047 | MS.gene52850 | PPI |
| MS.gene035194 | MS.gene52850 | PPI |
| MS.gene07203 | MS.gene52850 | PPI |
| MS.gene038750 | MS.gene52850 | PPI |
| MS.gene08109 | MS.gene52850 | PPI |
| MS.gene04079 | MS.gene52850 | PPI |
| MS.gene060573 | MS.gene52850 | PPI |
| MS.gene005611 | MS.gene52850 | PPI |
| MS.gene06380 | MS.gene52850 | PPI |
| MS.gene018651 | MS.gene52850 | PPI |
| MS.gene072703 | MS.gene52850 | PPI |
| MS.gene64888 | MS.gene52850 | PPI |
| MS.gene28806 | MS.gene52850 | PPI |
| MS.gene65331 | MS.gene52850 | PPI |
| MS.gene23437 | MS.gene52850 | PPI |
| MS.gene72658 | MS.gene52850 | PPI |
| MS.gene013296 | MS.gene52850 | PPI |
| MS.gene40912 | MS.gene52850 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52850.t1 | MTR_3g005810 | 97.906 | 573 | 12 | 0 | 1 | 573 | 134 | 706 | 0.0 | 1176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52850.t1 | AT4G09140 | 66.143 | 573 | 190 | 1 | 1 | 573 | 156 | 724 | 0.0 | 791 |
| MS.gene52850.t1 | AT4G09140 | 65.209 | 549 | 187 | 1 | 25 | 573 | 1 | 545 | 0.0 | 743 |
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGAGTTTGTGCTTAGTTT+AGG | 0.245169 | 3.3:-1515554 | MS.gene52850:CDS |
| CTGAGTTTGTGCTTAGTTTA+GGG | 0.247718 | 3.3:-1515553 | MS.gene52850:CDS |
| GTTCTAACCAACTTATTTAC+TGG | 0.258224 | 3.3:+1518957 | None:intergenic |
| TTTCAAACCACCTCCTTCTA+TGG | 0.261404 | 3.3:-1513600 | MS.gene52850:CDS |
| ACAATTGAAATATATATAAA+AGG | 0.283295 | 3.3:+1520584 | None:intergenic |
| GGTATGCTGGAGGAGTATTT+TGG | 0.292415 | 3.3:-1515656 | MS.gene52850:CDS |
| GATGCCATCAGAACAGTTTA+TGG | 0.295437 | 3.3:-1521308 | MS.gene52850:CDS |
| CGTGAAAGACTTGATTATAT+TGG | 0.303621 | 3.3:-1516698 | MS.gene52850:CDS |
| AGGCCCTGTGCTGCTGTAAA+AGG | 0.310412 | 3.3:-1522145 | MS.gene52850:CDS |
| ATAAAAGGCTTAGATGCTTT+AGG | 0.329580 | 3.3:+1520599 | None:intergenic |
| TGAGAATCTATTCTATAATA+TGG | 0.337500 | 3.3:-1521619 | MS.gene52850:CDS |
| TGGCATTCATATTGATGATC+AGG | 0.346186 | 3.3:-1515636 | MS.gene52850:CDS |
| TCAAACAGTTTCAGCTGCTC+TGG | 0.349043 | 3.3:-1515137 | MS.gene52850:CDS |
| GTTCGTACCTCTCTCTTTGT+TGG | 0.351550 | 3.3:+1520527 | None:intergenic |
| CAGCAAGTTCTCAGCCGTTT+TGG | 0.360552 | 3.3:-1516757 | MS.gene52850:CDS |
| TGTAAAAGGAACACAGATAA+TGG | 0.367128 | 3.3:-1522131 | MS.gene52850:intron |
| TTGAAATGTACGTGCATCAT+TGG | 0.368233 | 3.3:+1519928 | None:intergenic |
| TGATAACTTCCTGATTTAAG+AGG | 0.384335 | 3.3:+1519993 | None:intergenic |
| TGAGTTCTATAGAATCTTGT+AGG | 0.400669 | 3.3:+1517830 | None:intergenic |
| CCTCCTTCTATGGCTACTAA+TGG | 0.417391 | 3.3:-1513590 | MS.gene52850:CDS |
| ATGCCATCAGAACAGTTTAT+GGG | 0.417980 | 3.3:-1521307 | MS.gene52850:CDS |
| ATAGGTGAGCCTCTTAAATC+AGG | 0.431743 | 3.3:-1520002 | MS.gene52850:intron |
| TACTAATGGAACCTTTGTTA+AGG | 0.435371 | 3.3:-1513576 | MS.gene52850:CDS |
| CAATCCCTCACCAGATGGAT+TGG | 0.436586 | 3.3:+1515081 | None:intergenic |
| TTTATGTTTCTCAGATAGAC+TGG | 0.439450 | 3.3:-1520675 | MS.gene52850:intron |
| TTTACAGCAGCACAGGGCCT+AGG | 0.449578 | 3.3:+1522148 | None:intergenic |
| GAAAACAAAGGCTGGTATGC+TGG | 0.453883 | 3.3:-1515669 | MS.gene52850:CDS |
| GAAGACACTACAAAATTCTT+CGG | 0.456465 | 3.3:-1521586 | MS.gene52850:CDS |
| GTTCCATTAGTAGCCATAGA+AGG | 0.456923 | 3.3:+1513587 | None:intergenic |
| TTGCATGGGCCCAGCGTGAA+TGG | 0.464113 | 3.3:-1513661 | MS.gene52850:CDS |
| CATCTATCCTGTAGGAATGA+TGG | 0.469466 | 3.3:-1517114 | MS.gene52850:intron |
| TTTCTGAAGCTGAGATTGCA+TGG | 0.470561 | 3.3:-1513676 | MS.gene52850:CDS |
| TTAGCTGAATGGCATTGAAA+TGG | 0.472900 | 3.3:+1516734 | None:intergenic |
| CCGATGTTGCCCAATCCATC+TGG | 0.474634 | 3.3:-1515091 | MS.gene52850:CDS |
| TTCCCATACCCGGTGGGGTA+GGG | 0.475091 | 3.3:+1519087 | None:intergenic |
| GGCATTCATATTGATGATCA+GGG | 0.480584 | 3.3:-1515635 | MS.gene52850:CDS |
| AATATAATCAAGTCTTTCAC+GGG | 0.482818 | 3.3:+1516700 | None:intergenic |
| TCAGGGATGCGATCCATGTC+CGG | 0.485690 | 3.3:+1515572 | None:intergenic |
| TACCCGGTGGGGTAGGGCTG+AGG | 0.486411 | 3.3:+1519093 | None:intergenic |
| TTCTGAAGCTGAGATTGCAT+GGG | 0.487761 | 3.3:-1513675 | MS.gene52850:CDS |
| CCTAAACTAAGCACAAACTC+AGG | 0.490379 | 3.3:+1515554 | None:intergenic |
| CTTGATTATATTGGCACTGA+AGG | 0.492658 | 3.3:-1516689 | MS.gene52850:CDS |
| TCATACTTGTGGCAGACAGC+TGG | 0.499733 | 3.3:-1519158 | MS.gene52850:intron |
| TGGCATTGAAATGGCCAAAA+CGG | 0.505388 | 3.3:+1516743 | None:intergenic |
| GAGAATGCCTGTGATAATAC+TGG | 0.506862 | 3.3:-1515016 | MS.gene52850:intron |
| TAGAGATGGTGTCATGGAGC+AGG | 0.507145 | 3.3:-1522173 | None:intergenic |
| CAATATAATCAAGTCTTTCA+CGG | 0.507914 | 3.3:+1516699 | None:intergenic |
| CAAACAGTTTCAGCTGCTCT+GGG | 0.509172 | 3.3:-1515136 | MS.gene52850:CDS |
| TGTCAATTTCTCTTGCAGAA+AGG | 0.511158 | 3.3:-1521508 | MS.gene52850:intron |
| GGCTGGATCACTTAGCTGAA+TGG | 0.514732 | 3.3:+1516723 | None:intergenic |
| AATCCCTCACCAGATGGATT+GGG | 0.516217 | 3.3:+1515082 | None:intergenic |
| ATTGACATTGGTATGATGTA+TGG | 0.519238 | 3.3:+1521524 | None:intergenic |
| GGATGATTACTCAAAGATAG+TGG | 0.524793 | 3.3:-1521565 | MS.gene52850:CDS |
| GAAAGTGCCAGTAAATAAGT+TGG | 0.524800 | 3.3:-1518964 | MS.gene52850:CDS |
| TTGCAAATGTTGTAAACTTG+AGG | 0.530370 | 3.3:-1517013 | MS.gene52850:intron |
| TTTCCCATACCCGGTGGGGT+AGG | 0.533882 | 3.3:+1519086 | None:intergenic |
| GATCCATGTCCGGAGTATAC+TGG | 0.534406 | 3.3:+1515582 | None:intergenic |
| ATCAATAACAACTATGACCC+TGG | 0.540120 | 3.3:-1517781 | MS.gene52850:intron |
| TAACCTCAGCCCTACCCCAC+CGG | 0.548727 | 3.3:-1519096 | MS.gene52850:CDS |
| TTGTATTGACCATTCACGCT+GGG | 0.551512 | 3.3:+1513652 | None:intergenic |
| TATATTGGCACTGAAGGAAG+AGG | 0.554059 | 3.3:-1516683 | MS.gene52850:CDS |
| TAGGCGTGTAATCTGCCTGC+AGG | 0.557560 | 3.3:+1518921 | None:intergenic |
| ACAGATTCATTAGATCCTGC+AGG | 0.558475 | 3.3:-1518936 | MS.gene52850:CDS |
| TATCATTGAGAAGATACAAT+CGG | 0.558745 | 3.3:-1519975 | MS.gene52850:CDS |
| CGCAAACATTACCTTAACAA+AGG | 0.567048 | 3.3:+1513565 | None:intergenic |
| GATCTGCTGAAAACAAAGGC+TGG | 0.567731 | 3.3:-1515677 | MS.gene52850:CDS |
| ACATTGACATCAATATTCTC+AGG | 0.568147 | 3.3:+1520554 | None:intergenic |
| AAAAGCAATCCCTCACCAGA+TGG | 0.569149 | 3.3:+1515076 | None:intergenic |
| CCAGATGGATTGGGCAACAT+CGG | 0.571394 | 3.3:+1515091 | None:intergenic |
| GTAAAGCACTGCACATATGT+TGG | 0.572709 | 3.3:-1517086 | MS.gene52850:CDS |
| TCTGCAAGAGAAATTGACAT+TGG | 0.573051 | 3.3:+1521512 | None:intergenic |
| GACCCCATAAACTGTTCTGA+TGG | 0.573196 | 3.3:+1521304 | None:intergenic |
| GTCCATACCAGTATTATCAC+AGG | 0.577956 | 3.3:+1515009 | None:intergenic |
| TCTCAGATAGACTGGTTGAG+TGG | 0.579246 | 3.3:-1520667 | MS.gene52850:intron |
| GTATACTGGTCAAGTATCAC+AGG | 0.580775 | 3.3:+1515596 | None:intergenic |
| AAACATTTCCCATACCCGGT+GGG | 0.580830 | 3.3:+1519081 | None:intergenic |
| AACCTCAGCCCTACCCCACC+GGG | 0.583112 | 3.3:-1519095 | MS.gene52850:intron |
| GTGTCACTTTGAATGCAGTA+AGG | 0.584850 | 3.3:-1518863 | MS.gene52850:intron |
| GGCATCAAGCCTTGATGATG+TGG | 0.587629 | 3.3:+1521325 | None:intergenic |
| CGGTGATTGAATCAACATTG+AGG | 0.588119 | 3.3:-1519955 | MS.gene52850:CDS |
| GATGGATTGGGCAACATCGG+TGG | 0.588877 | 3.3:+1515094 | None:intergenic |
| CTCTCGTACTAATAAGAGCA+AGG | 0.589552 | 3.3:-1519123 | MS.gene52850:CDS |
| GCACTGCACATATGTTGGAA+TGG | 0.589828 | 3.3:-1517081 | MS.gene52850:CDS |
| AACATTTCCCATACCCGGTG+GGG | 0.591924 | 3.3:+1519082 | None:intergenic |
| ATTATCACAGGCATTCTCCA+TGG | 0.601535 | 3.3:+1515021 | None:intergenic |
| GTGTCATGGAGCAGGAACCT+AGG | 0.602402 | 3.3:-1522165 | None:intergenic |
| TTAGTAGCCATAGAAGGAGG+TGG | 0.606651 | 3.3:+1513593 | None:intergenic |
| CTAAACTAAGCACAAACTCA+GGG | 0.607686 | 3.3:+1515555 | None:intergenic |
| TTGCCCAATCCATCTGGTGA+GGG | 0.615762 | 3.3:-1515085 | MS.gene52850:CDS |
| AGGTTGATTGGGATGATGAA+AGG | 0.616203 | 3.3:-1515168 | MS.gene52850:CDS |
| ATACAGTAGCCACATCATCA+AGG | 0.617880 | 3.3:-1521334 | MS.gene52850:CDS |
| GCCTACATGCAAGTTATACC+TGG | 0.618767 | 3.3:-1518903 | MS.gene52850:CDS |
| GTTGCCCAATCCATCTGGTG+AGG | 0.621707 | 3.3:-1515086 | MS.gene52850:CDS |
| CTTGCTCTTATTAGTACGAG+AGG | 0.624813 | 3.3:+1519124 | None:intergenic |
| TATTCTATAATATGGCTGCA+AGG | 0.627616 | 3.3:-1521611 | MS.gene52850:CDS |
| GTTGTATTGACCATTCACGC+TGG | 0.629559 | 3.3:+1513651 | None:intergenic |
| TGACCAGTATACTCCGGACA+TGG | 0.629857 | 3.3:-1515585 | MS.gene52850:CDS |
| AGATAGTGGATGTTGTCAGT+CGG | 0.631946 | 3.3:-1521551 | MS.gene52850:CDS |
| CACAGATCTGCTGAAAACAA+AGG | 0.634034 | 3.3:-1515681 | MS.gene52850:CDS |
| TGCCATCAGAACAGTTTATG+GGG | 0.642839 | 3.3:-1521306 | MS.gene52850:CDS |
| TACATGCAAGTTATACCTGG+TGG | 0.650299 | 3.3:-1518900 | MS.gene52850:CDS |
| ACCAGGTATAACTTGCATGT+AGG | 0.658521 | 3.3:+1518902 | None:intergenic |
| GCTCCTTCGTTGTCTAACTG+AGG | 0.662697 | 3.3:+1517852 | None:intergenic |
| AACAAAGGCTGGTATGCTGG+AGG | 0.667613 | 3.3:-1515666 | MS.gene52850:CDS |
| CCATTAGTAGCCATAGAAGG+AGG | 0.673543 | 3.3:+1513590 | None:intergenic |
| GATACTTGACCAGTATACTC+CGG | 0.679952 | 3.3:-1515591 | MS.gene52850:CDS |
| TGTGCATCCAACAAAGAGAG+AGG | 0.681638 | 3.3:-1520534 | MS.gene52850:intron |
| TTGACATCAATATTCTCAGG+AGG | 0.686791 | 3.3:+1520557 | None:intergenic |
| ATATAATCAAGTCTTTCACG+GGG | 0.688244 | 3.3:+1516701 | None:intergenic |
| GATCCTCAGTTAGACAACGA+AGG | 0.703074 | 3.3:-1517855 | MS.gene52850:CDS |
| TGCTGCCAAGAAAATCACGA+TGG | 0.717164 | 3.3:-1521186 | MS.gene52850:CDS |
| TCGTACTAATAAGAGCAAGG+AGG | 0.754469 | 3.3:-1519120 | MS.gene52850:CDS |
| TATAATCAAGTCTTTCACGG+GGG | 0.793097 | 3.3:+1516702 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 1513562 | 1522182 | 1513562 | ID=MS.gene52850 |
| chr3.3 | mRNA | 1513562 | 1522182 | 1513562 | ID=MS.gene52850.t1;Parent=MS.gene52850 |
| chr3.3 | exon | 1522132 | 1522182 | 1522132 | ID=MS.gene52850.t1.exon1;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1522132 | 1522182 | 1522132 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1521509 | 1521643 | 1521509 | ID=MS.gene52850.t1.exon2;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1521509 | 1521643 | 1521509 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1521171 | 1521381 | 1521171 | ID=MS.gene52850.t1.exon3;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1521171 | 1521381 | 1521171 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1520535 | 1520683 | 1520535 | ID=MS.gene52850.t1.exon4;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1520535 | 1520683 | 1520535 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1519922 | 1520020 | 1519922 | ID=MS.gene52850.t1.exon5;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1519922 | 1520020 | 1519922 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1519096 | 1519165 | 1519096 | ID=MS.gene52850.t1.exon6;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1519096 | 1519165 | 1519096 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1518864 | 1518999 | 1518864 | ID=MS.gene52850.t1.exon7;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1518864 | 1518999 | 1518864 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1517782 | 1517876 | 1517782 | ID=MS.gene52850.t1.exon8;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1517782 | 1517876 | 1517782 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1517014 | 1517122 | 1517014 | ID=MS.gene52850.t1.exon9;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1517014 | 1517122 | 1517014 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1516630 | 1516795 | 1516630 | ID=MS.gene52850.t1.exon10;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1516630 | 1516795 | 1516630 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1515547 | 1515708 | 1515547 | ID=MS.gene52850.t1.exon11;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1515547 | 1515708 | 1515547 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1515017 | 1515188 | 1515017 | ID=MS.gene52850.t1.exon12;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1515017 | 1515188 | 1515017 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| chr3.3 | exon | 1513562 | 1513737 | 1513562 | ID=MS.gene52850.t1.exon13;Parent=MS.gene52850.t1 |
| chr3.3 | CDS | 1513562 | 1513737 | 1513562 | ID=cds.MS.gene52850.t1;Parent=MS.gene52850.t1 |
| Gene Sequence |
| Protein sequence |
