Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058842.t1 | XP_003629659.1 | 95.3 | 170 | 8 | 0 | 1 | 170 | 1 | 170 | 1.20E-79 | 305.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058842.t1 | G7LHU1 | 95.3 | 170 | 8 | 0 | 1 | 170 | 1 | 170 | 8.8e-80 | 305.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058842 | MS.gene27824 | 0.842459 | 2.55E-58 | -1.69E-46 |
| MS.gene058842 | MS.gene33123 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene058842 | MS.gene47586 | 0.818379 | 2.02E-52 | -1.69E-46 |
| MS.gene058842 | MS.gene47587 | 0.817906 | 2.59E-52 | -1.69E-46 |
| MS.gene058842 | MS.gene84657 | 0.802418 | 5.66E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058842.t1 | MTR_8g085150 | 95.294 | 170 | 8 | 0 | 1 | 170 | 1 | 170 | 1.77e-102 | 291 |
| MS.gene058842.t1 | MTR_8g087020 | 71.351 | 185 | 38 | 3 | 1 | 170 | 1 | 185 | 2.41e-83 | 244 |
| MS.gene058842.t1 | MTR_3g466150 | 28.662 | 157 | 102 | 3 | 1 | 156 | 1 | 148 | 5.45e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058842.t1 | AT1G29270 | 38.994 | 159 | 87 | 4 | 1 | 156 | 1 | 152 | 1.26e-26 | 99.0 |
| MS.gene058842.t1 | AT1G29270 | 41.860 | 129 | 66 | 3 | 30 | 156 | 7 | 128 | 2.02e-24 | 92.4 |
| MS.gene058842.t1 | AT2G40435 | 36.306 | 157 | 85 | 8 | 1 | 152 | 1 | 147 | 1.45e-13 | 65.1 |
| MS.gene058842.t1 | AT3G56220 | 32.919 | 161 | 94 | 7 | 1 | 156 | 1 | 152 | 7.25e-12 | 60.5 |
| MS.gene058842.t1 | AT2G40435 | 35.821 | 134 | 73 | 6 | 1 | 131 | 1 | 124 | 4.33e-11 | 57.8 |
| MS.gene058842.t1 | AT2G40435 | 35.821 | 134 | 73 | 6 | 1 | 131 | 1 | 124 | 4.65e-11 | 57.8 |
| MS.gene058842.t1 | AT3G56220 | 35.338 | 133 | 75 | 6 | 1 | 131 | 1 | 124 | 6.81e-11 | 57.8 |
Find 26 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAAAAGCCTCTAAAAT+TGG | 0.235711 | 4.3:+13760110 | None:intergenic |
| TAGAAAAGTACTCTTGTTAA+TGG | 0.243551 | 4.3:+13760441 | None:intergenic |
| GCAAGAGTTTCATGTGAAAA+TGG | 0.245398 | 4.3:-13760065 | MS.gene058842:CDS |
| ATGTTAAGATAGAGAAAATA+AGG | 0.252575 | 4.3:-13760187 | MS.gene058842:CDS |
| ACTCTCTTCTTGTGGCTATC+AGG | 0.266661 | 4.3:+13760365 | None:intergenic |
| GACATTATTCAATAAGCTAA+TGG | 0.324384 | 4.3:+13760342 | None:intergenic |
| GAAGCACTTCTAAAAGCTAT+TGG | 0.338458 | 4.3:-13759981 | MS.gene058842:CDS |
| AATATTAGAGATGGCTTGTA+AGG | 0.412524 | 4.3:-13760721 | None:intergenic |
| TGTTAAGATAGAGAAAATAA+GGG | 0.418872 | 4.3:-13760186 | MS.gene058842:CDS |
| CTTGTTAATGGAATTTCTGT+TGG | 0.425108 | 4.3:+13760453 | None:intergenic |
| TGGCTTGTAAGGTGCAGAAA+AGG | 0.441174 | 4.3:-13760710 | MS.gene058842:CDS |
| CATTATTGTTGGAGTTGGTA+AGG | 0.458218 | 4.3:+13760652 | None:intergenic |
| TGGAAAGCAGAGTGGTGAAA+AGG | 0.477572 | 4.3:-13759961 | MS.gene058842:CDS |
| GTAAAGGTCACATGTGAGAA+AGG | 0.513294 | 4.3:-13760149 | MS.gene058842:CDS |
| GGAAAGCAGAGTGGTGAAAA+GGG | 0.519153 | 4.3:-13759960 | MS.gene058842:CDS |
| TTCTATGGAAGCTATTATTG+TGG | 0.540454 | 4.3:-13760039 | MS.gene058842:CDS |
| GGAAGCTATTATTGTGGCTG+AGG | 0.546345 | 4.3:-13760033 | MS.gene058842:CDS |
| GGCTTGTAAGGTGCAGAAAA+GGG | 0.547566 | 4.3:-13760709 | MS.gene058842:CDS |
| TGAGAAAGGAGGTGACAAGT+TGG | 0.586233 | 4.3:-13760135 | MS.gene058842:CDS |
| GAATAATGTCAATGATAACA+AGG | 0.589484 | 4.3:-13760329 | MS.gene058842:intron |
| GCTAATGGACTCTCTTCTTG+TGG | 0.603658 | 4.3:+13760357 | None:intergenic |
| GTTAAGATAGAGAAAATAAG+GGG | 0.609952 | 4.3:-13760185 | MS.gene058842:CDS |
| AAGATAGAGAAAATAAGGGG+AGG | 0.627338 | 4.3:-13760182 | MS.gene058842:CDS |
| AGAGATGTGTGTGAATGTTG+AGG | 0.643020 | 4.3:-13760090 | MS.gene058842:CDS |
| AAAGCTATTGGAAAGCAGAG+TGG | 0.675693 | 4.3:-13759969 | MS.gene058842:CDS |
| AAGGTCACATGTGAGAAAGG+AGG | 0.686761 | 4.3:-13760146 | MS.gene058842:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTTTTTTTTATTATAAC+AGG | - | chr4.3:13760427-13760446 | MS.gene058842:CDS | 10.0% |
| !! | GAATGTAAAAAAAGAAAAAA+AGG | + | chr4.3:13760028-13760047 | None:intergenic | 15.0% |
| !!! | TGTTAATTTTACATTATTGT+TGG | + | chr4.3:13759999-13760018 | None:intergenic | 15.0% |
| !! | ATGTTAAGATAGAGAAAATA+AGG | - | chr4.3:13760450-13760469 | MS.gene058842:CDS | 20.0% |
| !! | TAATATTTATATATAGGCCT+TGG | + | chr4.3:13760384-13760403 | None:intergenic | 20.0% |
| !! | TCAGTGGAAAAAAATAATAA+TGG | + | chr4.3:13760059-13760078 | None:intergenic | 20.0% |
| !! | TGTTAAGATAGAGAAAATAA+GGG | - | chr4.3:13760451-13760470 | MS.gene058842:CDS | 20.0% |
| !!! | AAAAACATGTTATTTTTGCT+TGG | + | chr4.3:13760414-13760433 | None:intergenic | 20.0% |
| ! | GAATAATGTCAATGATAACA+AGG | - | chr4.3:13760308-13760327 | MS.gene058842:intron | 25.0% |
| ! | GACATTATTCAATAAGCTAA+TGG | + | chr4.3:13760298-13760317 | None:intergenic | 25.0% |
| ! | GATTAAATTTGTGAAATCAG+TGG | + | chr4.3:13760075-13760094 | None:intergenic | 25.0% |
| ! | GTTAAGATAGAGAAAATAAG+GGG | - | chr4.3:13760452-13760471 | MS.gene058842:CDS | 25.0% |
| ! | TATAGAAAAACACAAAACCA+AGG | - | chr4.3:13760364-13760383 | MS.gene058842:CDS | 25.0% |
| !! | TTTTACATTATTGTTGGAGT+TGG | + | chr4.3:13759993-13760012 | None:intergenic | 25.0% |
| !!! | ATGTGAAAATGGATTTTCTA+TGG | - | chr4.3:13760583-13760602 | MS.gene058842:intron | 25.0% |
| !!! | TAGAAAAGTACTCTTGTTAA+TGG | + | chr4.3:13760199-13760218 | None:intergenic | 25.0% |
| !!! | TTGTTACTTGTTTGTTTTGA+AGG | - | chr4.3:13760161-13760180 | MS.gene058842:CDS | 25.0% |
| CTTGTTAATGGAATTTCTGT+TGG | + | chr4.3:13760187-13760206 | None:intergenic | 30.0% | |
| TTCTATGGAAGCTATTATTG+TGG | - | chr4.3:13760598-13760617 | MS.gene058842:intron | 30.0% | |
| !! | TCTTCAAAAGCCTCTAAAAT+TGG | + | chr4.3:13760530-13760549 | None:intergenic | 30.0% |
| AAGATAGAGAAAATAAGGGG+AGG | - | chr4.3:13760455-13760474 | MS.gene058842:CDS | 35.0% | |
| GCAAGAGTTTCATGTGAAAA+TGG | - | chr4.3:13760572-13760591 | MS.gene058842:intron | 35.0% | |
| ! | AATAAGGGGAGGAACTTTTA+TGG | - | chr4.3:13760466-13760485 | MS.gene058842:intron | 35.0% |
| ! | GAAGCACTTCTAAAAGCTAT+TGG | - | chr4.3:13760656-13760675 | MS.gene058842:CDS | 35.0% |
| !! | CATTATTGTTGGAGTTGGTA+AGG | + | chr4.3:13759988-13760007 | None:intergenic | 35.0% |
| !!! | CAAGTTGGTTCCAATTTTAG+AGG | - | chr4.3:13760517-13760536 | MS.gene058842:intron | 35.0% |
| AAAGCTATTGGAAAGCAGAG+TGG | - | chr4.3:13760668-13760687 | MS.gene058842:CDS | 40.0% | |
| AGAGATGTGTGTGAATGTTG+AGG | - | chr4.3:13760547-13760566 | MS.gene058842:intron | 40.0% | |
| GTAAAGGTCACATGTGAGAA+AGG | - | chr4.3:13760488-13760507 | MS.gene058842:intron | 40.0% | |
| ! | GGGAGGAACTTTTATGGTAA+AGG | - | chr4.3:13760472-13760491 | MS.gene058842:intron | 40.0% |
| AAGGTCACATGTGAGAAAGG+AGG | - | chr4.3:13760491-13760510 | MS.gene058842:intron | 45.0% | |
| ACTCTCTTCTTGTGGCTATC+AGG | + | chr4.3:13760275-13760294 | None:intergenic | 45.0% | |
| GGAAAGCAGAGTGGTGAAAA+GGG | - | chr4.3:13760677-13760696 | MS.gene058842:CDS | 45.0% | |
| GGAAGCTATTATTGTGGCTG+AGG | - | chr4.3:13760604-13760623 | MS.gene058842:intron | 45.0% | |
| GGCTTGTAAGGTGCAGAAAA+GGG | - | chr4.3:13759928-13759947 | MS.gene058842:CDS | 45.0% | |
| TGAGAAAGGAGGTGACAAGT+TGG | - | chr4.3:13760502-13760521 | MS.gene058842:intron | 45.0% | |
| TGGAAAGCAGAGTGGTGAAA+AGG | - | chr4.3:13760676-13760695 | MS.gene058842:CDS | 45.0% | |
| ! | TGGCTTGTAAGGTGCAGAAA+AGG | - | chr4.3:13759927-13759946 | MS.gene058842:CDS | 45.0% |
| !! | GCTAATGGACTCTCTTCTTG+TGG | + | chr4.3:13760283-13760302 | None:intergenic | 45.0% |
| !!! | TTTTAGTAATATTTATATAT+AGG | + | chr4.3:13760390-13760409 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 13759926 | 13760733 | 13759926 | ID=MS.gene058842 |
| chr4.3 | mRNA | 13759926 | 13760733 | 13759926 | ID=MS.gene058842.t1;Parent=MS.gene058842 |
| chr4.3 | exon | 13760653 | 13760733 | 13760653 | ID=MS.gene058842.t1.exon1;Parent=MS.gene058842.t1 |
| chr4.3 | CDS | 13760653 | 13760733 | 13760653 | ID=cds.MS.gene058842.t1;Parent=MS.gene058842.t1 |
| chr4.3 | exon | 13760330 | 13760476 | 13760330 | ID=MS.gene058842.t1.exon2;Parent=MS.gene058842.t1 |
| chr4.3 | CDS | 13760330 | 13760476 | 13760330 | ID=cds.MS.gene058842.t1;Parent=MS.gene058842.t1 |
| chr4.3 | exon | 13759926 | 13760210 | 13759926 | ID=MS.gene058842.t1.exon3;Parent=MS.gene058842.t1 |
| chr4.3 | CDS | 13759926 | 13760210 | 13759926 | ID=cds.MS.gene058842.t1;Parent=MS.gene058842.t1 |
| Gene Sequence |
| Protein sequence |
