Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27824.t1 | XP_003629659.1 | 94.1 | 170 | 10 | 0 | 1 | 170 | 1 | 170 | 3.90E-78 | 300.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27824.t1 | G7LHU1 | 94.1 | 170 | 10 | 0 | 1 | 170 | 1 | 170 | 2.8e-78 | 300.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050901 | MS.gene27824 | 0.800225 | 1.59E-48 | -1.69E-46 |
| MS.gene051648 | MS.gene27824 | 0.824801 | 6.63E-54 | -1.69E-46 |
| MS.gene053144 | MS.gene27824 | 0.814678 | 1.36E-51 | -1.69E-46 |
| MS.gene053536 | MS.gene27824 | 0.8038 | 2.93E-49 | -1.69E-46 |
| MS.gene053538 | MS.gene27824 | 0.807764 | 4.31E-50 | -1.69E-46 |
| MS.gene058842 | MS.gene27824 | 0.842459 | 2.55E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27824.t1 | MTR_8g085150 | 94.118 | 170 | 10 | 0 | 1 | 170 | 1 | 170 | 1.63e-100 | 286 |
| MS.gene27824.t1 | MTR_8g087020 | 70.270 | 185 | 40 | 3 | 1 | 170 | 1 | 185 | 1.77e-81 | 239 |
| MS.gene27824.t1 | MTR_5g083810 | 35.669 | 157 | 86 | 7 | 1 | 154 | 25 | 169 | 3.91e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27824.t1 | AT1G29270 | 38.365 | 159 | 88 | 4 | 1 | 156 | 1 | 152 | 5.40e-26 | 97.4 |
| MS.gene27824.t1 | AT1G29270 | 41.085 | 129 | 67 | 3 | 30 | 156 | 7 | 128 | 6.91e-24 | 91.3 |
| MS.gene27824.t1 | AT2G40435 | 35.669 | 157 | 86 | 8 | 1 | 152 | 1 | 147 | 1.06e-12 | 62.8 |
Find 27 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAAAAGCCTCTAAAAT+TGG | 0.235711 | 4.3:-13628654 | None:intergenic |
| TAGAAAAGTACTCTTGTTAA+TGG | 0.243551 | 4.3:-13628325 | None:intergenic |
| GCAAGAGTTTCATGTGAAAA+TGG | 0.245398 | 4.3:+13628699 | MS.gene27824:CDS |
| ATGTTAAGATAGAGAAAATA+AGG | 0.252575 | 4.3:+13628577 | MS.gene27824:CDS |
| ACTCTCTTCTTGCGGCTATC+AGG | 0.318826 | 4.3:-13628401 | None:intergenic |
| GAAGCACTTCTAAAAGCTAT+TGG | 0.338458 | 4.3:+13628783 | MS.gene27824:CDS |
| GACATTATTCAATAAGCTAA+TGG | 0.375889 | 4.3:-13628424 | None:intergenic |
| AATATTAGAGATGGCTTGTA+AGG | 0.412524 | 4.3:+13628041 | None:intergenic |
| TGTTAAGATAGAGAAAATAA+GGG | 0.418872 | 4.3:+13628578 | MS.gene27824:CDS |
| CTTGTTAATGGAATTTCTGT+TGG | 0.425108 | 4.3:-13628313 | None:intergenic |
| TGGCTTGTAAGGTGCAGAAA+AGG | 0.441174 | 4.3:+13628052 | MS.gene27824:CDS |
| CATTATTGTTGGAGTTGGTA+AGG | 0.458218 | 4.3:-13628110 | None:intergenic |
| TGGAAAGCAGAGTGGTGAAA+AGG | 0.477572 | 4.3:+13628803 | MS.gene27824:CDS |
| GTAAAGGTCACATGTGAGAA+AGG | 0.513294 | 4.3:+13628615 | MS.gene27824:CDS |
| GGAAAGCAGAGTGGTGAAAA+GGG | 0.519153 | 4.3:+13628804 | MS.gene27824:CDS |
| GAGGATGAAAATCTTGATGT+TGG | 0.539252 | 4.3:+13628750 | MS.gene27824:CDS |
| TTCTATGGAAGCTATTATTG+TGG | 0.540454 | 4.3:+13628725 | MS.gene27824:CDS |
| GGAAGCTATTATTGTGGCTG+AGG | 0.546345 | 4.3:+13628731 | MS.gene27824:CDS |
| GGCTTGTAAGGTGCAGAAAA+GGG | 0.547566 | 4.3:+13628053 | MS.gene27824:CDS |
| TGAGAAAGGAGGTGACAAGT+TGG | 0.586233 | 4.3:+13628629 | MS.gene27824:CDS |
| GAATAATGTCTATGATAACA+AGG | 0.596304 | 4.3:+13628437 | MS.gene27824:CDS |
| GTTAAGATAGAGAAAATAAG+GGG | 0.609952 | 4.3:+13628579 | MS.gene27824:CDS |
| GCTAATGGACTCTCTTCTTG+CGG | 0.625040 | 4.3:-13628409 | None:intergenic |
| AAGATAGAGAAAATAAGGGG+AGG | 0.627338 | 4.3:+13628582 | MS.gene27824:CDS |
| AGAGATGTGTGTGAATGTTG+AGG | 0.643020 | 4.3:+13628674 | MS.gene27824:CDS |
| AAAGCTATTGGAAAGCAGAG+TGG | 0.675693 | 4.3:+13628795 | MS.gene27824:CDS |
| AAGGTCACATGTGAGAAAGG+AGG | 0.686761 | 4.3:+13628618 | MS.gene27824:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTTTTTTTTTATTATAAC+AGG | + | chr4.3:13628554-13628573 | MS.gene27824:intron | 10.0% |
| !! | GAATGTAAAAAAAGAAAAAA+AGG | - | chr4.3:13628153-13628172 | None:intergenic | 15.0% |
| !!! | TGTTAATTTTACATTATTGT+TGG | - | chr4.3:13628124-13628143 | None:intergenic | 15.0% |
| !! | ATGTTAAGATAGAGAAAATA+AGG | + | chr4.3:13628577-13628596 | MS.gene27824:CDS | 20.0% |
| !! | TAATATTTATATATAGGCCT+TGG | - | chr4.3:13628512-13628531 | None:intergenic | 20.0% |
| !! | TCAGTGGAAAAAAATAATAA+TGG | - | chr4.3:13628184-13628203 | None:intergenic | 20.0% |
| !! | TGTTAAGATAGAGAAAATAA+GGG | + | chr4.3:13628578-13628597 | MS.gene27824:CDS | 20.0% |
| !!! | AAAAACATGTTATTTTTGCT+TGG | - | chr4.3:13628542-13628561 | None:intergenic | 20.0% |
| ! | GAATAATGTCTATGATAACA+AGG | + | chr4.3:13628437-13628456 | MS.gene27824:CDS | 25.0% |
| ! | GACATTATTCAATAAGCTAA+TGG | - | chr4.3:13628427-13628446 | None:intergenic | 25.0% |
| ! | GATTAAATTTGTGAAATCAG+TGG | - | chr4.3:13628200-13628219 | None:intergenic | 25.0% |
| ! | GTTAAGATAGAGAAAATAAG+GGG | + | chr4.3:13628579-13628598 | MS.gene27824:CDS | 25.0% |
| ! | TATAGAAAAACACAAAACCA+AGG | + | chr4.3:13628492-13628511 | MS.gene27824:intron | 25.0% |
| !! | TTTTACATTATTGTTGGAGT+TGG | - | chr4.3:13628118-13628137 | None:intergenic | 25.0% |
| !!! | ATGTGAAAATGGATTTTCTA+TGG | + | chr4.3:13628710-13628729 | MS.gene27824:CDS | 25.0% |
| !!! | TAGAAAAGTACTCTTGTTAA+TGG | - | chr4.3:13628328-13628347 | None:intergenic | 25.0% |
| !!! | TTGTTACTTGTTTGTTTTGA+AGG | + | chr4.3:13628290-13628309 | MS.gene27824:intron | 25.0% |
| CTTGTTAATGGAATTTCTGT+TGG | - | chr4.3:13628316-13628335 | None:intergenic | 30.0% | |
| TTCTATGGAAGCTATTATTG+TGG | + | chr4.3:13628725-13628744 | MS.gene27824:CDS | 30.0% | |
| !! | TCTTCAAAAGCCTCTAAAAT+TGG | - | chr4.3:13628657-13628676 | None:intergenic | 30.0% |
| AAGATAGAGAAAATAAGGGG+AGG | + | chr4.3:13628582-13628601 | MS.gene27824:CDS | 35.0% | |
| GAGGATGAAAATCTTGATGT+TGG | + | chr4.3:13628750-13628769 | MS.gene27824:CDS | 35.0% | |
| GCAAGAGTTTCATGTGAAAA+TGG | + | chr4.3:13628699-13628718 | MS.gene27824:CDS | 35.0% | |
| ! | AATAAGGGGAGGAACTTTTA+TGG | + | chr4.3:13628593-13628612 | MS.gene27824:CDS | 35.0% |
| ! | GAAGCACTTCTAAAAGCTAT+TGG | + | chr4.3:13628783-13628802 | MS.gene27824:CDS | 35.0% |
| !! | CATTATTGTTGGAGTTGGTA+AGG | - | chr4.3:13628113-13628132 | None:intergenic | 35.0% |
| !!! | CAAGTTGGTTCCAATTTTAG+AGG | + | chr4.3:13628644-13628663 | MS.gene27824:CDS | 35.0% |
| AAAGCTATTGGAAAGCAGAG+TGG | + | chr4.3:13628795-13628814 | MS.gene27824:CDS | 40.0% | |
| AGAGATGTGTGTGAATGTTG+AGG | + | chr4.3:13628674-13628693 | MS.gene27824:CDS | 40.0% | |
| GTAAAGGTCACATGTGAGAA+AGG | + | chr4.3:13628615-13628634 | MS.gene27824:CDS | 40.0% | |
| ! | GGGAGGAACTTTTATGGTAA+AGG | + | chr4.3:13628599-13628618 | MS.gene27824:CDS | 40.0% |
| AAGGTCACATGTGAGAAAGG+AGG | + | chr4.3:13628618-13628637 | MS.gene27824:CDS | 45.0% | |
| GGAAAGCAGAGTGGTGAAAA+GGG | + | chr4.3:13628804-13628823 | MS.gene27824:CDS | 45.0% | |
| GGAAGCTATTATTGTGGCTG+AGG | + | chr4.3:13628731-13628750 | MS.gene27824:CDS | 45.0% | |
| GGCTTGTAAGGTGCAGAAAA+GGG | + | chr4.3:13628053-13628072 | MS.gene27824:CDS | 45.0% | |
| TGAGAAAGGAGGTGACAAGT+TGG | + | chr4.3:13628629-13628648 | MS.gene27824:CDS | 45.0% | |
| TGGAAAGCAGAGTGGTGAAA+AGG | + | chr4.3:13628803-13628822 | MS.gene27824:CDS | 45.0% | |
| ! | TGGCTTGTAAGGTGCAGAAA+AGG | + | chr4.3:13628052-13628071 | MS.gene27824:CDS | 45.0% |
| !! | GCTAATGGACTCTCTTCTTG+CGG | - | chr4.3:13628412-13628431 | None:intergenic | 45.0% |
| !!! | TTTTAGTAATATTTATATAT+AGG | - | chr4.3:13628518-13628537 | None:intergenic | 5.0% |
| ACTCTCTTCTTGCGGCTATC+AGG | - | chr4.3:13628404-13628423 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 13628051 | 13628860 | 13628051 | ID=MS.gene27824 |
| chr4.3 | mRNA | 13628051 | 13628860 | 13628051 | ID=MS.gene27824.t1;Parent=MS.gene27824 |
| chr4.3 | exon | 13628051 | 13628131 | 13628051 | ID=MS.gene27824.t1.exon1;Parent=MS.gene27824.t1 |
| chr4.3 | CDS | 13628051 | 13628131 | 13628051 | ID=cds.MS.gene27824.t1;Parent=MS.gene27824.t1 |
| chr4.3 | exon | 13628312 | 13628458 | 13628312 | ID=MS.gene27824.t1.exon2;Parent=MS.gene27824.t1 |
| chr4.3 | CDS | 13628312 | 13628458 | 13628312 | ID=cds.MS.gene27824.t1;Parent=MS.gene27824.t1 |
| chr4.3 | exon | 13628576 | 13628860 | 13628576 | ID=MS.gene27824.t1.exon3;Parent=MS.gene27824.t1 |
| chr4.3 | CDS | 13628576 | 13628860 | 13628576 | ID=cds.MS.gene27824.t1;Parent=MS.gene27824.t1 |
| Gene Sequence |
| Protein sequence |
