Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84657.t1 | XP_013449092.1 | 96.2 | 106 | 1 | 1 | 1 | 103 | 641 | 746 | 1.40E-41 | 178.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84657.t1 | A0A072U007 | 96.2 | 106 | 1 | 1 | 1 | 103 | 641 | 746 | 9.9e-42 | 178.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene84657 | 0.812515 | 4.08E-51 | -1.69E-46 |
| MS.gene050689 | MS.gene84657 | 0.807068 | 6.06E-50 | -1.69E-46 |
| MS.gene051478 | MS.gene84657 | 0.803197 | 3.91E-49 | -1.69E-46 |
| MS.gene051781 | MS.gene84657 | 0.861671 | 8.71E-64 | -1.69E-46 |
| MS.gene051784 | MS.gene84657 | 0.844059 | 9.56E-59 | -1.69E-46 |
| MS.gene051787 | MS.gene84657 | 0.84458 | 6.92E-59 | -1.69E-46 |
| MS.gene052155 | MS.gene84657 | -0.808054 | 3.74E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene84657 | 0.800745 | 1.25E-48 | -1.69E-46 |
| MS.gene053144 | MS.gene84657 | 0.822536 | 2.25E-53 | -1.69E-46 |
| MS.gene053536 | MS.gene84657 | 0.826584 | 2.50E-54 | -1.69E-46 |
| MS.gene053538 | MS.gene84657 | 0.819611 | 1.06E-52 | -1.69E-46 |
| MS.gene053581 | MS.gene84657 | 0.807243 | 5.56E-50 | -1.69E-46 |
| MS.gene055162 | MS.gene84657 | 0.801791 | 7.62E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene84657 | 0.802251 | 6.13E-49 | -1.69E-46 |
| MS.gene055580 | MS.gene84657 | -0.840677 | 7.53E-58 | -1.69E-46 |
| MS.gene056043 | MS.gene84657 | -0.826338 | 2.87E-54 | -1.69E-46 |
| MS.gene057705 | MS.gene84657 | 0.805518 | 1.28E-49 | -1.69E-46 |
| MS.gene058842 | MS.gene84657 | 0.802418 | 5.66E-49 | -1.69E-46 |
| MS.gene05894 | MS.gene84657 | 0.825597 | 4.30E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84657.t1 | MTR_7g066730 | 97.170 | 106 | 0 | 1 | 1 | 103 | 641 | 746 | 1.39e-62 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGGTGGAGGCGGAGGAGG+AGG | 0.283550 | 7.3:+38231505 | MS.gene84657:CDS |
| ATGGGAATGGGTGGAGGCGG+AGG | 0.360541 | 7.3:+38231499 | MS.gene84657:CDS |
| AGTTTGTGTTGGAGAGGAAC+AGG | 0.362327 | 7.3:+38231596 | MS.gene84657:CDS |
| GGAATGGGTGGAGGCGGAGG+AGG | 0.362698 | 7.3:+38231502 | MS.gene84657:CDS |
| GGCACCGTTTCTCCGGCCAC+TGG | 0.385742 | 7.3:-38231683 | None:intergenic |
| CTGATTGGACCTTCCTTTCG+AGG | 0.389614 | 7.3:-38231704 | None:intergenic |
| CCTACCAACAGCATGGATAA+TGG | 0.399873 | 7.3:+38231634 | MS.gene84657:CDS |
| AGCAATAGAATGAGCCTGAT+TGG | 0.414209 | 7.3:-38231719 | None:intergenic |
| AATAGCAGTAATGGGTATCT+TGG | 0.444044 | 7.3:+38231556 | MS.gene84657:CDS |
| CTCGAAAGGAAGGTCCAATC+AGG | 0.455417 | 7.3:+38231705 | MS.gene84657:CDS |
| AGTGATGTTAATAGCAGTAA+TGG | 0.463161 | 7.3:+38231547 | MS.gene84657:CDS |
| GTGATGTTAATAGCAGTAAT+GGG | 0.468047 | 7.3:+38231548 | MS.gene84657:CDS |
| ACGCCCAGTGGCCGGAGAAA+CGG | 0.468591 | 7.3:+38231679 | MS.gene84657:CDS |
| CCGAGCATGGGAATGGGTGG+AGG | 0.468607 | 7.3:+38231493 | MS.gene84657:CDS |
| CTTTCGAGGCACCGTTTCTC+CGG | 0.469423 | 7.3:-38231690 | None:intergenic |
| AGCATGGGAATGGGTGGAGG+CGG | 0.475408 | 7.3:+38231496 | MS.gene84657:CDS |
| CTATTGCTAGAAGTGTACTT+AGG | 0.477905 | 7.3:+38231734 | MS.gene84657:CDS |
| TGGGTGGAGGCGGAGGAGGA+GGG | 0.479086 | 7.3:+38231506 | MS.gene84657:CDS |
| AGGAAGGGATGAGTTTGTGT+TGG | 0.494812 | 7.3:+38231585 | MS.gene84657:CDS |
| GAATGCACCGAGCATGGGAA+TGG | 0.495277 | 7.3:+38231486 | MS.gene84657:CDS |
| CCTCCACCCATTCCCATGCT+CGG | 0.495888 | 7.3:-38231493 | None:intergenic |
| TTGGAGGAATGCACCGAGCA+TGG | 0.498605 | 7.3:+38231480 | MS.gene84657:CDS |
| AATGGGTATCTTGGCAGAAA+AGG | 0.499659 | 7.3:+38231565 | MS.gene84657:CDS |
| TCTACTTAACGCCCAGTGGC+CGG | 0.503962 | 7.3:+38231671 | MS.gene84657:CDS |
| GCACCGTTTCTCCGGCCACT+GGG | 0.516795 | 7.3:-38231682 | None:intergenic |
| AATGCACCGAGCATGGGAAT+GGG | 0.539420 | 7.3:+38231487 | MS.gene84657:CDS |
| TGGAGGAATGCACCGAGCAT+GGG | 0.549427 | 7.3:+38231481 | MS.gene84657:CDS |
| CGGAGAAACGGTGCCTCGAA+AGG | 0.550290 | 7.3:+38231691 | MS.gene84657:CDS |
| GGGATGAGTTTGTGTTGGAG+AGG | 0.556006 | 7.3:+38231590 | MS.gene84657:CDS |
| CCATTATCCATGCTGTTGGT+AGG | 0.559362 | 7.3:-38231634 | None:intergenic |
| TGGATAATGGTGGTCTCTTG+AGG | 0.561050 | 7.3:+38231647 | MS.gene84657:CDS |
| GAAACGGTGCCTCGAAAGGA+AGG | 0.570868 | 7.3:+38231695 | MS.gene84657:CDS |
| GCAATAGTAGTGTCAGTTGG+AGG | 0.577464 | 7.3:+38231464 | MS.gene84657:CDS |
| GGTATCTTGGCAGAAAAGGA+AGG | 0.581764 | 7.3:+38231569 | MS.gene84657:CDS |
| GGAGCAATAGTAGTGTCAGT+TGG | 0.584572 | 7.3:+38231461 | MS.gene84657:CDS |
| ACCACCATTATCCATGCTGT+TGG | 0.585092 | 7.3:-38231638 | None:intergenic |
| GCACCGAGCATGGGAATGGG+TGG | 0.602722 | 7.3:+38231490 | MS.gene84657:CDS |
| ATATTCTCCTACCAACAGCA+TGG | 0.640802 | 7.3:+38231627 | MS.gene84657:CDS |
| GTTTCGATAGGAAGATGTTG+AGG | 0.641118 | 7.3:+38231440 | None:intergenic |
| GTATCTTGGCAGAAAAGGAA+GGG | 0.654337 | 7.3:+38231570 | MS.gene84657:CDS |
| AGGCTCTACTTAACGCCCAG+TGG | 0.658591 | 7.3:+38231667 | MS.gene84657:CDS |
| ACCAACAGCATGGATAATGG+TGG | 0.681176 | 7.3:+38231637 | MS.gene84657:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTGATGTTAATAGCAGTAA+TGG | + | chr7.3:38231547-38231566 | MS.gene84657:CDS | 30.0% | |
| GTGATGTTAATAGCAGTAAT+GGG | + | chr7.3:38231548-38231567 | MS.gene84657:CDS | 30.0% | |
| ! | CTATTGCTAGAAGTGTACTT+AGG | + | chr7.3:38231734-38231753 | MS.gene84657:CDS | 35.0% |
| !! | AATAGCAGTAATGGGTATCT+TGG | + | chr7.3:38231556-38231575 | MS.gene84657:CDS | 35.0% |
| AATGGGTATCTTGGCAGAAA+AGG | + | chr7.3:38231565-38231584 | MS.gene84657:CDS | 40.0% | |
| AGCAATAGAATGAGCCTGAT+TGG | - | chr7.3:38231722-38231741 | None:intergenic | 40.0% | |
| ATATTCTCCTACCAACAGCA+TGG | + | chr7.3:38231627-38231646 | MS.gene84657:CDS | 40.0% | |
| GTATCTTGGCAGAAAAGGAA+GGG | + | chr7.3:38231570-38231589 | MS.gene84657:CDS | 40.0% | |
| ACCACCATTATCCATGCTGT+TGG | - | chr7.3:38231641-38231660 | None:intergenic | 45.0% | |
| AGGAAGGGATGAGTTTGTGT+TGG | + | chr7.3:38231585-38231604 | MS.gene84657:CDS | 45.0% | |
| AGTTTGTGTTGGAGAGGAAC+AGG | + | chr7.3:38231596-38231615 | MS.gene84657:CDS | 45.0% | |
| CCATTATCCATGCTGTTGGT+AGG | - | chr7.3:38231637-38231656 | None:intergenic | 45.0% | |
| CCTACCAACAGCATGGATAA+TGG | + | chr7.3:38231634-38231653 | MS.gene84657:CDS | 45.0% | |
| GCAATAGTAGTGTCAGTTGG+AGG | + | chr7.3:38231464-38231483 | MS.gene84657:CDS | 45.0% | |
| GGAGCAATAGTAGTGTCAGT+TGG | + | chr7.3:38231461-38231480 | MS.gene84657:CDS | 45.0% | |
| GGTATCTTGGCAGAAAAGGA+AGG | + | chr7.3:38231569-38231588 | MS.gene84657:CDS | 45.0% | |
| ! | ACCAACAGCATGGATAATGG+TGG | + | chr7.3:38231637-38231656 | MS.gene84657:CDS | 45.0% |
| ! | TGGATAATGGTGGTCTCTTG+AGG | + | chr7.3:38231647-38231666 | MS.gene84657:CDS | 45.0% |
| AATGCACCGAGCATGGGAAT+GGG | + | chr7.3:38231487-38231506 | MS.gene84657:CDS | 50.0% | |
| CTCGAAAGGAAGGTCCAATC+AGG | + | chr7.3:38231705-38231724 | MS.gene84657:CDS | 50.0% | |
| CTGATTGGACCTTCCTTTCG+AGG | - | chr7.3:38231707-38231726 | None:intergenic | 50.0% | |
| GGGATGAGTTTGTGTTGGAG+AGG | + | chr7.3:38231590-38231609 | MS.gene84657:CDS | 50.0% | |
| AGGCTCTACTTAACGCCCAG+TGG | + | chr7.3:38231667-38231686 | MS.gene84657:CDS | 55.0% | |
| GAAACGGTGCCTCGAAAGGA+AGG | + | chr7.3:38231695-38231714 | MS.gene84657:CDS | 55.0% | |
| GAATGCACCGAGCATGGGAA+TGG | + | chr7.3:38231486-38231505 | MS.gene84657:CDS | 55.0% | |
| TCTACTTAACGCCCAGTGGC+CGG | + | chr7.3:38231671-38231690 | MS.gene84657:CDS | 55.0% | |
| TGGAGGAATGCACCGAGCAT+GGG | + | chr7.3:38231481-38231500 | MS.gene84657:CDS | 55.0% | |
| TTGGAGGAATGCACCGAGCA+TGG | + | chr7.3:38231480-38231499 | MS.gene84657:CDS | 55.0% | |
| !! | CTTTCGAGGCACCGTTTCTC+CGG | - | chr7.3:38231693-38231712 | None:intergenic | 55.0% |
| !! | GGAGGAGGAGGGATTTTTGG+CGG | + | chr7.3:38231517-38231536 | MS.gene84657:CDS | 55.0% |
| !!! | GAGGAGGAGGGATTTTTGGC+GGG | + | chr7.3:38231518-38231537 | MS.gene84657:CDS | 55.0% |
| AGCATGGGAATGGGTGGAGG+CGG | + | chr7.3:38231496-38231515 | MS.gene84657:CDS | 60.0% | |
| CCTCCACCCATTCCCATGCT+CGG | - | chr7.3:38231496-38231515 | None:intergenic | 60.0% | |
| CGGAGAAACGGTGCCTCGAA+AGG | + | chr7.3:38231691-38231710 | MS.gene84657:CDS | 60.0% | |
| ! | GGCGGAGGAGGAGGGATTTT+TGG | + | chr7.3:38231514-38231533 | MS.gene84657:CDS | 60.0% |
| !!! | GAGGAGGGATTTTTGGCGGG+AGG | + | chr7.3:38231521-38231540 | MS.gene84657:CDS | 60.0% |
| ACGCCCAGTGGCCGGAGAAA+CGG | + | chr7.3:38231679-38231698 | MS.gene84657:CDS | 65.0% | |
| ATGGGAATGGGTGGAGGCGG+AGG | + | chr7.3:38231499-38231518 | MS.gene84657:CDS | 65.0% | |
| CCGAGCATGGGAATGGGTGG+AGG | + | chr7.3:38231493-38231512 | MS.gene84657:CDS | 65.0% | |
| GCACCGAGCATGGGAATGGG+TGG | + | chr7.3:38231490-38231509 | MS.gene84657:CDS | 65.0% | |
| GCACCGTTTCTCCGGCCACT+GGG | - | chr7.3:38231685-38231704 | None:intergenic | 65.0% | |
| ATGGGTGGAGGCGGAGGAGG+AGG | + | chr7.3:38231505-38231524 | MS.gene84657:CDS | 70.0% | |
| GGAATGGGTGGAGGCGGAGG+AGG | + | chr7.3:38231502-38231521 | MS.gene84657:CDS | 70.0% | |
| GGCACCGTTTCTCCGGCCAC+TGG | - | chr7.3:38231686-38231705 | None:intergenic | 70.0% | |
| TGGGTGGAGGCGGAGGAGGA+GGG | + | chr7.3:38231506-38231525 | MS.gene84657:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 38231454 | 38231765 | 38231454 | ID=MS.gene84657 |
| chr7.3 | mRNA | 38231454 | 38231765 | 38231454 | ID=MS.gene84657.t1;Parent=MS.gene84657 |
| chr7.3 | exon | 38231454 | 38231765 | 38231454 | ID=MS.gene84657.t1.exon1;Parent=MS.gene84657.t1 |
| chr7.3 | CDS | 38231454 | 38231765 | 38231454 | ID=cds.MS.gene84657.t1;Parent=MS.gene84657.t1 |
| Gene Sequence |
| Protein sequence |
