Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33123.t1 | AFK38628.1 | 85.6 | 187 | 24 | 1 | 1 | 187 | 1 | 184 | 6.00E-80 | 307 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33123.t1 | Q9SK27 | 48.1 | 181 | 84 | 4 | 4 | 184 | 8 | 178 | 8.9e-40 | 164.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33123.t1 | I3SED6 | 85.6 | 187 | 24 | 1 | 1 | 187 | 1 | 184 | 4.4e-80 | 307.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049328 | MS.gene33123 | 0.827077 | 1.91E-54 | -1.69E-46 |
| MS.gene049418 | MS.gene33123 | 0.872961 | 2.13E-67 | -1.69E-46 |
| MS.gene049711 | MS.gene33123 | 0.806238 | 9.06E-50 | -1.69E-46 |
| MS.gene049989 | MS.gene33123 | 0.81042 | 1.16E-50 | -1.69E-46 |
| MS.gene050297 | MS.gene33123 | 0.84251 | 2.47E-58 | -1.69E-46 |
| MS.gene050310 | MS.gene33123 | 0.847162 | 1.38E-59 | -1.69E-46 |
| MS.gene050648 | MS.gene33123 | 0.834772 | 2.47E-56 | -1.69E-46 |
| MS.gene050901 | MS.gene33123 | 0.822525 | 2.26E-53 | -1.69E-46 |
| MS.gene05092 | MS.gene33123 | 0.818799 | 1.62E-52 | -1.69E-46 |
| MS.gene051018 | MS.gene33123 | 0.828158 | 1.05E-54 | -1.69E-46 |
| MS.gene051021 | MS.gene33123 | 0.800437 | 1.44E-48 | -1.69E-46 |
| MS.gene051067 | MS.gene33123 | 0.817982 | 2.49E-52 | -1.69E-46 |
| MS.gene051199 | MS.gene33123 | 0.802064 | 6.70E-49 | -1.69E-46 |
| MS.gene051263 | MS.gene33123 | 0.852865 | 3.49E-61 | -1.69E-46 |
| MS.gene051478 | MS.gene33123 | 0.930365 | 1.82E-93 | -1.69E-46 |
| MS.gene05157 | MS.gene33123 | 0.823106 | 1.66E-53 | -1.69E-46 |
| MS.gene051648 | MS.gene33123 | 0.823384 | 1.43E-53 | -1.69E-46 |
| MS.gene051671 | MS.gene33123 | 0.819233 | 1.29E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene33123 | 0.804659 | 1.94E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene33123 | 0.832073 | 1.16E-55 | -1.69E-46 |
| MS.gene052731 | MS.gene33123 | 0.803925 | 2.76E-49 | -1.69E-46 |
| MS.gene052732 | MS.gene33123 | 0.823631 | 1.25E-53 | -1.69E-46 |
| MS.gene053144 | MS.gene33123 | 0.85381 | 1.87E-61 | -1.69E-46 |
| MS.gene053536 | MS.gene33123 | 0.823862 | 1.10E-53 | -1.69E-46 |
| MS.gene053538 | MS.gene33123 | 0.820609 | 6.27E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene33123 | 0.822269 | 2.59E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene33123 | 0.845518 | 3.86E-59 | -1.69E-46 |
| MS.gene054643 | MS.gene33123 | 0.812066 | 5.11E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene33123 | 0.801392 | 9.20E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene33123 | 0.852029 | 6.03E-61 | -1.69E-46 |
| MS.gene055580 | MS.gene33123 | -0.808112 | 3.63E-50 | -1.69E-46 |
| MS.gene055764 | MS.gene33123 | 0.81604 | 6.79E-52 | -1.69E-46 |
| MS.gene055957 | MS.gene33123 | 0.808768 | 2.63E-50 | -1.69E-46 |
| MS.gene056169 | MS.gene33123 | 0.839746 | 1.32E-57 | -1.69E-46 |
| MS.gene056228 | MS.gene33123 | 0.822462 | 2.34E-53 | -1.69E-46 |
| MS.gene057004 | MS.gene33123 | 0.81989 | 9.16E-53 | -1.69E-46 |
| MS.gene057082 | MS.gene33123 | 0.800532 | 1.38E-48 | -1.69E-46 |
| MS.gene057483 | MS.gene33123 | 0.807279 | 5.46E-50 | -1.69E-46 |
| MS.gene058197 | MS.gene33123 | 0.847645 | 1.01E-59 | -1.69E-46 |
| MS.gene058198 | MS.gene33123 | 0.847966 | 8.27E-60 | -1.69E-46 |
| MS.gene058842 | MS.gene33123 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene05894 | MS.gene33123 | 0.844946 | 5.52E-59 | -1.69E-46 |
| MS.gene059300 | MS.gene33123 | 0.861515 | 9.72E-64 | -1.69E-46 |
| MS.gene059424 | MS.gene33123 | 0.816865 | 4.44E-52 | -1.69E-46 |
| MS.gene060519 | MS.gene33123 | 0.830944 | 2.21E-55 | -1.69E-46 |
| MS.gene06057 | MS.gene33123 | 0.874553 | 6.17E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene33123 | MS.gene031754 | PPI |
| MS.gene33123 | MS.gene038783 | PPI |
| MS.gene33123 | MS.gene065888 | PPI |
| MS.gene006553 | MS.gene33123 | PPI |
| MS.gene032997 | MS.gene33123 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33123.t1 | MTR_1g014120 | 94.652 | 187 | 7 | 1 | 1 | 187 | 1 | 184 | 3.40e-108 | 307 |
| MS.gene33123.t1 | MTR_3g105930 | 64.474 | 152 | 47 | 3 | 25 | 176 | 24 | 168 | 3.18e-63 | 193 |
| MS.gene33123.t1 | MTR_7g090170 | 56.757 | 148 | 40 | 4 | 25 | 172 | 24 | 147 | 1.33e-49 | 157 |
| MS.gene33123.t1 | MTR_8g463180 | 47.107 | 121 | 58 | 3 | 12 | 132 | 11 | 125 | 1.16e-34 | 124 |
| MS.gene33123.t1 | MTR_4g081100 | 48.624 | 109 | 55 | 1 | 24 | 132 | 28 | 135 | 1.82e-33 | 119 |
| MS.gene33123.t1 | MTR_2g079030 | 42.105 | 152 | 84 | 3 | 10 | 161 | 8 | 155 | 2.37e-33 | 124 |
| MS.gene33123.t1 | MTR_6g022170 | 48.182 | 110 | 54 | 2 | 21 | 129 | 26 | 133 | 2.39e-31 | 112 |
| MS.gene33123.t1 | MTR_2g090575 | 41.212 | 165 | 78 | 7 | 11 | 163 | 11 | 168 | 4.19e-29 | 110 |
| MS.gene33123.t1 | MTR_2g090580 | 37.714 | 175 | 89 | 4 | 30 | 187 | 29 | 200 | 9.90e-29 | 106 |
| MS.gene33123.t1 | MTR_4g078410 | 43.846 | 130 | 69 | 3 | 25 | 153 | 22 | 148 | 1.47e-28 | 105 |
| MS.gene33123.t1 | MTR_2g101300 | 37.908 | 153 | 82 | 6 | 12 | 162 | 11 | 152 | 3.46e-28 | 104 |
| MS.gene33123.t1 | MTR_4g124280 | 46.465 | 99 | 53 | 0 | 32 | 130 | 28 | 126 | 1.32e-27 | 104 |
| MS.gene33123.t1 | MTR_4g111640 | 36.957 | 138 | 78 | 4 | 1 | 132 | 1 | 135 | 2.29e-26 | 99.8 |
| MS.gene33123.t1 | MTR_4g077787 | 36.913 | 149 | 81 | 5 | 1 | 143 | 1 | 142 | 3.24e-26 | 99.4 |
| MS.gene33123.t1 | MTR_8g086360 | 35.870 | 184 | 101 | 5 | 7 | 178 | 3 | 181 | 3.41e-26 | 99.4 |
| MS.gene33123.t1 | MTR_2g083250 | 40.909 | 110 | 62 | 3 | 25 | 132 | 23 | 131 | 3.44e-25 | 98.6 |
| MS.gene33123.t1 | MTR_8g094990 | 37.278 | 169 | 90 | 4 | 12 | 167 | 8 | 173 | 3.55e-25 | 99.0 |
| MS.gene33123.t1 | MTR_8g095013 | 43.220 | 118 | 62 | 3 | 11 | 128 | 11 | 123 | 9.47e-24 | 95.5 |
| MS.gene33123.t1 | MTR_4g130800 | 38.168 | 131 | 78 | 3 | 1 | 129 | 1 | 130 | 1.76e-23 | 94.4 |
| MS.gene33123.t1 | MTR_4g130780 | 36.508 | 126 | 75 | 1 | 8 | 128 | 4 | 129 | 4.35e-23 | 91.3 |
| MS.gene33123.t1 | MTR_4g130800 | 43.119 | 109 | 60 | 2 | 22 | 129 | 22 | 129 | 6.72e-23 | 92.4 |
| MS.gene33123.t1 | MTR_4g112310 | 44.706 | 85 | 45 | 1 | 48 | 132 | 3 | 85 | 1.12e-22 | 88.6 |
| MS.gene33123.t1 | MTR_2g088990 | 34.194 | 155 | 90 | 5 | 15 | 168 | 13 | 156 | 1.95e-22 | 90.5 |
| MS.gene33123.t1 | MTR_5g006040 | 26.705 | 176 | 121 | 3 | 12 | 187 | 11 | 178 | 1.62e-21 | 87.0 |
| MS.gene33123.t1 | MTR_1g077790 | 38.776 | 98 | 59 | 1 | 32 | 128 | 32 | 129 | 2.03e-21 | 87.0 |
| MS.gene33123.t1 | MTR_8g095020 | 39.844 | 128 | 64 | 5 | 30 | 148 | 27 | 150 | 1.14e-20 | 87.4 |
| MS.gene33123.t1 | MTR_3g092170 | 34.591 | 159 | 86 | 3 | 12 | 163 | 8 | 155 | 1.72e-20 | 86.3 |
| MS.gene33123.t1 | MTR_4g114870 | 32.738 | 168 | 92 | 2 | 32 | 178 | 194 | 361 | 1.32e-19 | 85.1 |
| MS.gene33123.t1 | MTR_4g114870 | 31.852 | 135 | 89 | 2 | 12 | 146 | 13 | 144 | 9.58e-18 | 80.1 |
| MS.gene33123.t1 | MTR_1g104800 | 35.833 | 120 | 75 | 1 | 49 | 166 | 43 | 162 | 2.57e-18 | 78.6 |
| MS.gene33123.t1 | MTR_0334s0010 | 37.705 | 122 | 65 | 4 | 15 | 128 | 7 | 125 | 2.55e-17 | 76.3 |
| MS.gene33123.t1 | MTR_6g080660 | 34.211 | 114 | 68 | 3 | 17 | 130 | 18 | 124 | 3.20e-17 | 76.3 |
| MS.gene33123.t1 | MTR_4g114830 | 34.375 | 96 | 61 | 2 | 25 | 118 | 25 | 120 | 8.65e-17 | 73.9 |
| MS.gene33123.t1 | MTR_6g083240 | 33.083 | 133 | 81 | 4 | 37 | 165 | 35 | 163 | 1.05e-16 | 74.3 |
| MS.gene33123.t1 | MTR_8g099220 | 38.144 | 97 | 59 | 1 | 32 | 128 | 32 | 127 | 1.19e-15 | 70.1 |
| MS.gene33123.t1 | MTR_8g007020 | 35.938 | 128 | 73 | 4 | 5 | 130 | 2 | 122 | 1.67e-15 | 71.2 |
| MS.gene33123.t1 | MTR_1g090190 | 30.968 | 155 | 94 | 6 | 32 | 184 | 26 | 169 | 3.41e-15 | 70.5 |
| MS.gene33123.t1 | MTR_8g007035 | 35.938 | 128 | 73 | 4 | 5 | 130 | 2 | 122 | 6.25e-15 | 70.1 |
| MS.gene33123.t1 | MTR_4g067200 | 27.869 | 122 | 77 | 3 | 17 | 128 | 9 | 129 | 1.20e-14 | 68.6 |
| MS.gene33123.t1 | MTR_4g066110 | 27.869 | 122 | 77 | 3 | 17 | 128 | 9 | 129 | 1.20e-14 | 68.6 |
| MS.gene33123.t1 | MTR_2g025580 | 33.621 | 116 | 63 | 2 | 50 | 164 | 44 | 146 | 4.05e-14 | 67.4 |
| MS.gene33123.t1 | MTR_7g086100 | 32.558 | 129 | 81 | 3 | 7 | 134 | 4 | 127 | 5.18e-14 | 68.2 |
| MS.gene33123.t1 | MTR_6g023760 | 37.755 | 98 | 55 | 4 | 32 | 128 | 29 | 121 | 8.97e-14 | 66.6 |
| MS.gene33123.t1 | MTR_7g086090 | 31.405 | 121 | 78 | 2 | 14 | 134 | 12 | 127 | 1.33e-12 | 64.3 |
| MS.gene33123.t1 | MTR_7g086230 | 29.167 | 120 | 80 | 2 | 15 | 134 | 13 | 127 | 3.45e-12 | 62.4 |
| MS.gene33123.t1 | MTR_7g086280 | 29.457 | 129 | 85 | 3 | 7 | 134 | 4 | 127 | 3.82e-12 | 62.0 |
| MS.gene33123.t1 | MTR_8g089110 | 31.897 | 116 | 75 | 2 | 11 | 126 | 7 | 118 | 5.47e-12 | 60.5 |
| MS.gene33123.t1 | MTR_3g099540 | 38.272 | 81 | 49 | 1 | 48 | 128 | 42 | 121 | 5.52e-12 | 60.8 |
| MS.gene33123.t1 | MTR_3g099580 | 39.506 | 81 | 48 | 1 | 48 | 128 | 41 | 120 | 7.69e-12 | 61.6 |
| MS.gene33123.t1 | MTR_1g090420 | 27.097 | 155 | 105 | 4 | 11 | 161 | 13 | 163 | 1.42e-11 | 61.2 |
| MS.gene33123.t1 | MTR_3g099570 | 38.272 | 81 | 49 | 1 | 48 | 128 | 41 | 120 | 1.57e-11 | 60.5 |
| MS.gene33123.t1 | MTR_6g013420 | 30.597 | 134 | 78 | 5 | 7 | 131 | 10 | 137 | 7.03e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33123.t1 | AT4G31840 | 49.189 | 185 | 78 | 4 | 8 | 187 | 2 | 175 | 6.48e-57 | 177 |
| MS.gene33123.t1 | AT2G25060 | 49.714 | 175 | 76 | 5 | 11 | 184 | 15 | 178 | 1.18e-54 | 172 |
| MS.gene33123.t1 | AT5G25090 | 51.639 | 122 | 59 | 0 | 11 | 132 | 9 | 130 | 3.31e-45 | 147 |
| MS.gene33123.t1 | AT4G30590 | 58.095 | 105 | 44 | 0 | 28 | 132 | 28 | 132 | 6.21e-44 | 144 |
| MS.gene33123.t1 | AT5G57920 | 44.079 | 152 | 82 | 3 | 26 | 177 | 21 | 169 | 1.99e-43 | 143 |
| MS.gene33123.t1 | AT5G57920 | 44.079 | 152 | 82 | 3 | 26 | 177 | 53 | 201 | 2.02e-43 | 144 |
| MS.gene33123.t1 | AT5G57920 | 44.079 | 152 | 82 | 3 | 26 | 177 | 20 | 168 | 2.13e-43 | 143 |
| MS.gene33123.t1 | AT2G23990 | 49.324 | 148 | 61 | 3 | 8 | 150 | 3 | 141 | 3.18e-40 | 135 |
| MS.gene33123.t1 | AT2G23990 | 43.713 | 167 | 61 | 4 | 8 | 150 | 3 | 160 | 1.73e-35 | 124 |
| MS.gene33123.t1 | AT3G20570 | 40.491 | 163 | 83 | 5 | 12 | 164 | 15 | 173 | 7.25e-35 | 122 |
| MS.gene33123.t1 | AT5G14345 | 57.143 | 91 | 37 | 1 | 40 | 130 | 28 | 116 | 9.20e-31 | 109 |
| MS.gene33123.t1 | AT5G53870 | 40.252 | 159 | 84 | 5 | 10 | 161 | 9 | 163 | 3.46e-30 | 113 |
| MS.gene33123.t1 | AT5G53870 | 40.252 | 159 | 84 | 5 | 10 | 161 | 9 | 163 | 4.29e-30 | 113 |
| MS.gene33123.t1 | AT4G28365 | 39.506 | 162 | 89 | 6 | 13 | 168 | 11 | 169 | 8.25e-29 | 106 |
| MS.gene33123.t1 | AT4G27520 | 43.802 | 121 | 64 | 3 | 12 | 132 | 16 | 132 | 1.11e-27 | 106 |
| MS.gene33123.t1 | AT3G18590 | 45.000 | 120 | 60 | 4 | 12 | 129 | 12 | 127 | 1.98e-27 | 102 |
| MS.gene33123.t1 | AT4G32490 | 34.857 | 175 | 85 | 5 | 13 | 166 | 11 | 177 | 3.05e-25 | 97.8 |
| MS.gene33123.t1 | AT1G48940 | 38.095 | 126 | 70 | 3 | 13 | 132 | 6 | 129 | 1.71e-24 | 94.7 |
| MS.gene33123.t1 | AT2G31050 | 36.170 | 141 | 84 | 3 | 5 | 145 | 6 | 140 | 6.06e-22 | 88.6 |
| MS.gene33123.t1 | AT1G79800 | 34.459 | 148 | 85 | 4 | 8 | 147 | 8 | 151 | 7.77e-22 | 88.2 |
| MS.gene33123.t1 | AT1G64640 | 35.043 | 117 | 75 | 1 | 32 | 148 | 36 | 151 | 1.12e-20 | 85.1 |
| MS.gene33123.t1 | AT2G26720 | 33.813 | 139 | 84 | 3 | 11 | 149 | 12 | 142 | 7.18e-20 | 83.2 |
| MS.gene33123.t1 | AT2G32300 | 35.714 | 140 | 74 | 5 | 25 | 160 | 23 | 150 | 6.33e-19 | 82.0 |
| MS.gene33123.t1 | AT2G32300 | 35.714 | 140 | 74 | 5 | 25 | 160 | 53 | 180 | 2.07e-18 | 80.9 |
| MS.gene33123.t1 | AT5G20230 | 34.426 | 122 | 76 | 3 | 26 | 145 | 23 | 142 | 6.48e-17 | 75.5 |
| MS.gene33123.t1 | AT5G26330 | 36.250 | 80 | 51 | 0 | 50 | 129 | 43 | 122 | 1.22e-15 | 71.6 |
| MS.gene33123.t1 | AT5G07475 | 28.022 | 182 | 118 | 6 | 12 | 187 | 10 | 184 | 3.34e-15 | 70.5 |
| MS.gene33123.t1 | AT1G72230 | 31.967 | 122 | 72 | 2 | 48 | 159 | 38 | 158 | 9.25e-14 | 66.6 |
| MS.gene33123.t1 | AT3G60270 | 35.354 | 99 | 59 | 2 | 32 | 130 | 29 | 122 | 1.26e-13 | 66.2 |
| MS.gene33123.t1 | AT3G27200 | 29.839 | 124 | 76 | 3 | 11 | 129 | 5 | 122 | 1.09e-12 | 63.5 |
| MS.gene33123.t1 | AT1G45063 | 31.933 | 119 | 75 | 3 | 12 | 129 | 14 | 127 | 2.71e-12 | 63.9 |
| MS.gene33123.t1 | AT1G45063 | 31.933 | 119 | 75 | 3 | 12 | 129 | 14 | 127 | 3.84e-12 | 63.9 |
| MS.gene33123.t1 | AT1G22480 | 41.333 | 75 | 43 | 1 | 56 | 130 | 42 | 115 | 4.33e-11 | 59.3 |
| MS.gene33123.t1 | AT3G17675 | 26.804 | 97 | 67 | 1 | 32 | 128 | 35 | 127 | 9.90e-11 | 57.4 |
Find 45 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATCATCTTGTTCTCTTT+TGG | 0.177913 | 1.1:+7562641 | MS.gene33123:CDS |
| TTGGTTCTTGTTGTTCTCTT+TGG | 0.235363 | 1.1:+7562660 | MS.gene33123:CDS |
| CTGTGGTTGTCATGAGTCTA+AGG | 0.276529 | 1.1:+7563383 | MS.gene33123:CDS |
| CCCATCATTGTAGTGTTTGA+TGG | 0.297797 | 1.1:-7563275 | None:intergenic |
| TCTCCACTGATGTAGTAGTA+TGG | 0.331847 | 1.1:-7563328 | None:intergenic |
| AAAGTGAGATTTGATCATTC+TGG | 0.347650 | 1.1:+7563304 | MS.gene33123:CDS |
| AATCTGATTCTCTCAACAAA+TGG | 0.402045 | 1.1:+7562751 | MS.gene33123:CDS |
| GGAGCAACAGCTGAGCCAGC+TGG | 0.404423 | 1.1:-7563466 | None:intergenic |
| CTACTACATCAGTGGAGAAA+AGG | 0.404851 | 1.1:+7563333 | MS.gene33123:CDS |
| CCCATCAAACACTACAATGA+TGG | 0.417208 | 1.1:+7563274 | MS.gene33123:CDS |
| CCATCATTGTAGTGTTTGAT+GGG | 0.419117 | 1.1:-7563274 | None:intergenic |
| TGCATGGAAGGTTCCTTCTT+CGG | 0.420616 | 1.1:+7562728 | MS.gene33123:CDS |
| TACTTAGATGGTCTCCAACT+TGG | 0.420901 | 1.1:-7562791 | None:intergenic |
| CCTTCACCAGCTGAAGTTGA+TGG | 0.422615 | 1.1:+7563442 | MS.gene33123:CDS |
| GCAAGGTGGTGGTGTATTTG+TGG | 0.435276 | 1.1:+7563516 | MS.gene33123:CDS |
| AAAAGTGAAAATTACTTAGA+TGG | 0.435763 | 1.1:-7562803 | None:intergenic |
| AAAACAGTTGCACCACTTGT+TGG | 0.440200 | 1.1:-7563493 | None:intergenic |
| GCAGCTAAGGATATCTTGCT+TGG | 0.450579 | 1.1:+7562693 | MS.gene33123:CDS |
| TACTACATCAGTGGAGAAAA+GGG | 0.477152 | 1.1:+7563334 | MS.gene33123:CDS |
| ATCTGATTCTCTCAACAAAT+GGG | 0.481996 | 1.1:+7562752 | MS.gene33123:CDS |
| TGTGGTTGTCATGAGTCTAA+GGG | 0.500303 | 1.1:+7563384 | MS.gene33123:CDS |
| GTTGCACCACTTGTTGGAGC+TGG | 0.502974 | 1.1:-7563487 | None:intergenic |
| TCAAGTGTTCGTTTCCAAGT+TGG | 0.513908 | 1.1:+7562777 | MS.gene33123:CDS |
| AGAGAATCAGATTCCGAAGA+AGG | 0.516269 | 1.1:-7562741 | None:intergenic |
| ACTTCAGCTGGTGAAGGTGC+TGG | 0.517037 | 1.1:-7563436 | None:intergenic |
| CCATCAAACACTACAATGAT+GGG | 0.517725 | 1.1:+7563275 | MS.gene33123:CDS |
| GCTGAAGTTGATGGTCCAGC+TGG | 0.527675 | 1.1:+7563451 | MS.gene33123:CDS |
| GCTGGACCATCAACTTCAGC+TGG | 0.530028 | 1.1:-7563448 | None:intergenic |
| TCAGTTTGGAAATATGAAGC+TGG | 0.536642 | 1.1:+7563193 | MS.gene33123:intron |
| GAAAAGGGTCATTGTGAGAA+GGG | 0.553149 | 1.1:+7563349 | MS.gene33123:CDS |
| GTGGTTGTCATGAGTCTAAG+GGG | 0.573266 | 1.1:+7563385 | MS.gene33123:CDS |
| CCATCAACTTCAGCTGGTGA+AGG | 0.581807 | 1.1:-7563442 | None:intergenic |
| GGTGGTGTATTTGTGGCTGT+AGG | 0.582095 | 1.1:+7563523 | MS.gene33123:CDS |
| AGAAAAGGGTCATTGTGAGA+AGG | 0.594361 | 1.1:+7563348 | MS.gene33123:CDS |
| GTTGCTCCAGCTCCAACAAG+TGG | 0.607631 | 1.1:+7563481 | MS.gene33123:CDS |
| GCTAAGGATATCTTGCTTGG+TGG | 0.617121 | 1.1:+7562696 | MS.gene33123:CDS |
| GGTCCATACTACTACATCAG+TGG | 0.620422 | 1.1:+7563325 | MS.gene33123:CDS |
| TGGCAAAACTGATGCATGGA+AGG | 0.623442 | 1.1:+7562716 | MS.gene33123:CDS |
| TGGTTGTCATGAGTCTAAGG+GGG | 0.630347 | 1.1:+7563386 | MS.gene33123:CDS |
| TAGGAGTGTTTGTTGCCATG+TGG | 0.640277 | 1.1:+7563542 | MS.gene33123:CDS |
| TTGGTGGCAAAACTGATGCA+TGG | 0.676457 | 1.1:+7562712 | MS.gene33123:CDS |
| TTCAAATCAGAAAAGCCACA+TGG | 0.691376 | 1.1:-7563557 | None:intergenic |
| GGTTGTCATGAGTCTAAGGG+GGG | 0.691450 | 1.1:+7563387 | MS.gene33123:CDS |
| GTTGTCATGAGTCTAAGGGG+GGG | 0.700416 | 1.1:+7563388 | MS.gene33123:CDS |
| GAAGGGTCAGAAGCTTACTG+TGG | 0.745278 | 1.1:+7563366 | MS.gene33123:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATCACATAAAAATAAAAAT+TGG | - | chr1.1:7563127-7563146 | None:intergenic | 15.0% |
| !!! | AATATAAAAAAATTGGGTTT+TGG | - | chr1.1:7563047-7563066 | None:intergenic | 15.0% |
| !!! | ACAATAAAGAAACAATTTTA+AGG | - | chr1.1:7562947-7562966 | None:intergenic | 15.0% |
| !!! | TGAATTTTTACTGATAATTA+AGG | - | chr1.1:7562884-7562903 | None:intergenic | 15.0% |
| !!! | TGAATTTTTAGTGATAAAAA+AGG | - | chr1.1:7562842-7562861 | None:intergenic | 15.0% |
| !! | AAAAGTGAAAATTACTTAGA+TGG | - | chr1.1:7562806-7562825 | None:intergenic | 20.0% |
| !! | GGTGGAAATATAAAAAAATT+GGG | - | chr1.1:7563053-7563072 | None:intergenic | 20.0% |
| !! | TGGTGGAAATATAAAAAAAT+TGG | - | chr1.1:7563054-7563073 | None:intergenic | 20.0% |
| !!! | TTTTTACATTTTCATCATCT+TGG | + | chr1.1:7563010-7563029 | MS.gene33123:intron | 20.0% |
| ! | ATGAACAATAAAGTAGTTAG+TGG | - | chr1.1:7563163-7563182 | None:intergenic | 25.0% |
| ! | TTGAAAACAAAATACCAAGA+TGG | - | chr1.1:7563074-7563093 | None:intergenic | 25.0% |
| !! | TTTTTATATTTCCACCATCT+TGG | + | chr1.1:7563057-7563076 | MS.gene33123:intron | 25.0% |
| !!! | TCATATTTTTGTTCTCAGTT+TGG | + | chr1.1:7563179-7563198 | MS.gene33123:intron | 25.0% |
| AAAACAAAATACCAAGATGG+TGG | - | chr1.1:7563071-7563090 | None:intergenic | 30.0% | |
| AAAGTGAGATTTGATCATTC+TGG | + | chr1.1:7563304-7563323 | MS.gene33123:CDS | 30.0% | |
| AATCTGATTCTCTCAACAAA+TGG | + | chr1.1:7562751-7562770 | MS.gene33123:CDS | 30.0% | |
| ATCTGATTCTCTCAACAAAT+GGG | + | chr1.1:7562752-7562771 | MS.gene33123:CDS | 30.0% | |
| ! | TTCATCATCTTGTTCTCTTT+TGG | + | chr1.1:7562641-7562660 | MS.gene33123:CDS | 30.0% |
| CCATCAAACACTACAATGAT+GGG | + | chr1.1:7563275-7563294 | MS.gene33123:CDS | 35.0% | |
| CCATCATTGTAGTGTTTGAT+GGG | - | chr1.1:7563277-7563296 | None:intergenic | 35.0% | |
| TACTACATCAGTGGAGAAAA+GGG | + | chr1.1:7563334-7563353 | MS.gene33123:CDS | 35.0% | |
| TCAGTTTGGAAATATGAAGC+TGG | + | chr1.1:7563193-7563212 | MS.gene33123:intron | 35.0% | |
| ! | TTCTGTTTTGCAAGTGAGTA+AGG | + | chr1.1:7563222-7563241 | MS.gene33123:CDS | 35.0% |
| !! | TTGGTTCTTGTTGTTCTCTT+TGG | + | chr1.1:7562660-7562679 | MS.gene33123:CDS | 35.0% |
| AAAACAGTTGCACCACTTGT+TGG | - | chr1.1:7563496-7563515 | None:intergenic | 40.0% | |
| AGAAAAGGGTCATTGTGAGA+AGG | + | chr1.1:7563348-7563367 | MS.gene33123:CDS | 40.0% | |
| AGAGAATCAGATTCCGAAGA+AGG | - | chr1.1:7562744-7562763 | None:intergenic | 40.0% | |
| CCCATCAAACACTACAATGA+TGG | + | chr1.1:7563274-7563293 | MS.gene33123:CDS | 40.0% | |
| CCCATCATTGTAGTGTTTGA+TGG | - | chr1.1:7563278-7563297 | None:intergenic | 40.0% | |
| CTACTACATCAGTGGAGAAA+AGG | + | chr1.1:7563333-7563352 | MS.gene33123:CDS | 40.0% | |
| GAAAAGGGTCATTGTGAGAA+GGG | + | chr1.1:7563349-7563368 | MS.gene33123:CDS | 40.0% | |
| GCTGGTGAAAAAGCAACAAT+GGG | - | chr1.1:7563421-7563440 | None:intergenic | 40.0% | |
| TACTTAGATGGTCTCCAACT+TGG | - | chr1.1:7562794-7562813 | None:intergenic | 40.0% | |
| TCAAGTGTTCGTTTCCAAGT+TGG | + | chr1.1:7562777-7562796 | MS.gene33123:CDS | 40.0% | |
| TCTCCACTGATGTAGTAGTA+TGG | - | chr1.1:7563331-7563350 | None:intergenic | 40.0% | |
| TGCTGGTGAAAAAGCAACAA+TGG | - | chr1.1:7563422-7563441 | None:intergenic | 40.0% | |
| TGTGGTTGTCATGAGTCTAA+GGG | + | chr1.1:7563384-7563403 | MS.gene33123:CDS | 40.0% | |
| CTGTGGTTGTCATGAGTCTA+AGG | + | chr1.1:7563383-7563402 | MS.gene33123:CDS | 45.0% | |
| GCAGCTAAGGATATCTTGCT+TGG | + | chr1.1:7562693-7562712 | MS.gene33123:CDS | 45.0% | |
| GCTAAGGATATCTTGCTTGG+TGG | + | chr1.1:7562696-7562715 | MS.gene33123:CDS | 45.0% | |
| GGTCCATACTACTACATCAG+TGG | + | chr1.1:7563325-7563344 | MS.gene33123:CDS | 45.0% | |
| GTGGTTGTCATGAGTCTAAG+GGG | + | chr1.1:7563385-7563404 | MS.gene33123:CDS | 45.0% | |
| TGCATGGAAGGTTCCTTCTT+CGG | + | chr1.1:7562728-7562747 | MS.gene33123:CDS | 45.0% | |
| TGGCAAAACTGATGCATGGA+AGG | + | chr1.1:7562716-7562735 | MS.gene33123:CDS | 45.0% | |
| TGGTTGTCATGAGTCTAAGG+GGG | + | chr1.1:7563386-7563405 | MS.gene33123:CDS | 45.0% | |
| ! | AGTGGTGCAACTGTTTTGCA+AGG | + | chr1.1:7563499-7563518 | MS.gene33123:CDS | 45.0% |
| ! | TGGTTGTGCTTTTGCAGCTA+AGG | + | chr1.1:7562680-7562699 | MS.gene33123:CDS | 45.0% |
| ! | TTGGTGGCAAAACTGATGCA+TGG | + | chr1.1:7562712-7562731 | MS.gene33123:CDS | 45.0% |
| !! | TAGGAGTGTTTGTTGCCATG+TGG | + | chr1.1:7563542-7563561 | MS.gene33123:CDS | 45.0% |
| CCATCAACTTCAGCTGGTGA+AGG | - | chr1.1:7563445-7563464 | None:intergenic | 50.0% | |
| CCTTCACCAGCTGAAGTTGA+TGG | + | chr1.1:7563442-7563461 | MS.gene33123:CDS | 50.0% | |
| GAAGGGTCAGAAGCTTACTG+TGG | + | chr1.1:7563366-7563385 | MS.gene33123:CDS | 50.0% | |
| GGTGGTGTATTTGTGGCTGT+AGG | + | chr1.1:7563523-7563542 | MS.gene33123:CDS | 50.0% | |
| GGTTGTCATGAGTCTAAGGG+GGG | + | chr1.1:7563387-7563406 | MS.gene33123:CDS | 50.0% | |
| GTTGTCATGAGTCTAAGGGG+GGG | + | chr1.1:7563388-7563407 | MS.gene33123:CDS | 50.0% | |
| ! | GCAACTGTTTTGCAAGGTGG+TGG | + | chr1.1:7563505-7563524 | MS.gene33123:CDS | 50.0% |
| ! | GCAAGGTGGTGGTGTATTTG+TGG | + | chr1.1:7563516-7563535 | MS.gene33123:CDS | 50.0% |
| ! | GGTGCAACTGTTTTGCAAGG+TGG | + | chr1.1:7563502-7563521 | MS.gene33123:CDS | 50.0% |
| GCTGGACCATCAACTTCAGC+TGG | - | chr1.1:7563451-7563470 | None:intergenic | 55.0% | |
| GTTGCTCCAGCTCCAACAAG+TGG | + | chr1.1:7563481-7563500 | MS.gene33123:CDS | 55.0% | |
| TGAGTCTAAGGGGGGGAAGT+AGG | + | chr1.1:7563395-7563414 | MS.gene33123:CDS | 55.0% | |
| ! | ACTTCAGCTGGTGAAGGTGC+TGG | - | chr1.1:7563439-7563458 | None:intergenic | 55.0% |
| ! | GTTGCACCACTTGTTGGAGC+TGG | - | chr1.1:7563490-7563509 | None:intergenic | 55.0% |
| !! | GCTGAAGTTGATGGTCCAGC+TGG | + | chr1.1:7563451-7563470 | MS.gene33123:CDS | 55.0% |
| GGAGCAACAGCTGAGCCAGC+TGG | - | chr1.1:7563469-7563488 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 7562624 | 7563573 | 7562624 | ID=MS.gene33123 |
| chr1.1 | mRNA | 7562624 | 7563573 | 7562624 | ID=MS.gene33123.t1;Parent=MS.gene33123 |
| chr1.1 | exon | 7562624 | 7562810 | 7562624 | ID=MS.gene33123.t1.exon1;Parent=MS.gene33123.t1 |
| chr1.1 | CDS | 7562624 | 7562810 | 7562624 | ID=cds.MS.gene33123.t1;Parent=MS.gene33123.t1 |
| chr1.1 | exon | 7563197 | 7563573 | 7563197 | ID=MS.gene33123.t1.exon2;Parent=MS.gene33123.t1 |
| chr1.1 | CDS | 7563197 | 7563573 | 7563197 | ID=cds.MS.gene33123.t1;Parent=MS.gene33123.t1 |
| Gene Sequence |
| Protein sequence |
