Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071711.t1 | AAL33588.1 | 100 | 62 | 0 | 0 | 1 | 62 | 59 | 120 | 1.90E-30 | 141 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071711.t1 | O23255 | 100.0 | 62 | 0 | 0 | 1 | 62 | 79 | 140 | 4.6e-33 | 141.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071711.t1 | M5XCZ6 | 100.0 | 62 | 0 | 0 | 1 | 62 | 79 | 140 | 1.4e-30 | 141.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049293 | MS.gene071711 | 0.817016 | 4.10E-52 | -1.69E-46 |
| MS.gene049669 | MS.gene071711 | -0.808386 | 3.18E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene071711 | -0.824566 | 7.53E-54 | -1.69E-46 |
| MS.gene049873 | MS.gene071711 | -0.830012 | 3.73E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene071711 | -0.813321 | 2.72E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene071711 | -0.801181 | 1.02E-48 | -1.69E-46 |
| MS.gene051633 | MS.gene071711 | -0.807368 | 5.23E-50 | -1.69E-46 |
| MS.gene052048 | MS.gene071711 | 0.808573 | 2.90E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene071711 | -0.814524 | 1.47E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene071711 | 0.840583 | 7.97E-58 | -1.69E-46 |
| MS.gene052358 | MS.gene071711 | 0.862738 | 4.10E-64 | -1.69E-46 |
| MS.gene052376 | MS.gene071711 | -0.817459 | 3.26E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene071711 | -0.805536 | 1.27E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene071711 | -0.808428 | 3.11E-50 | -1.69E-46 |
| MS.gene053294 | MS.gene071711 | -0.813947 | 1.98E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene071711 | -0.817868 | 2.64E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene071711 | -0.802516 | 5.41E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene071711 | -0.844455 | 7.48E-59 | -1.69E-46 |
| MS.gene057079 | MS.gene071711 | -0.833262 | 5.90E-56 | -1.69E-46 |
| MS.gene057555 | MS.gene071711 | -0.811898 | 5.56E-51 | -1.69E-46 |
| MS.gene057967 | MS.gene071711 | -0.805923 | 1.06E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene071711 | -0.832492 | 9.17E-56 | -1.69E-46 |
| MS.gene059362 | MS.gene071711 | 0.881546 | 2.19E-70 | -1.69E-46 |
| MS.gene059364 | MS.gene071711 | 0.921378 | 3.86E-88 | -1.69E-46 |
| MS.gene05954 | MS.gene071711 | -0.847982 | 8.19E-60 | -1.69E-46 |
| MS.gene060572 | MS.gene071711 | -0.834984 | 2.18E-56 | -1.69E-46 |
| MS.gene060634 | MS.gene071711 | 0.813681 | 2.26E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene071711 | 0.869013 | 4.26E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTACTGAACGTGCTCTTGAC+TGG | 0.316338 | 4.2:-13248798 | MS.gene071711:CDS |
| TTGAAGAGTCTCACCCTTCC+AGG | 0.334073 | 4.2:+13248834 | None:intergenic |
| TACTGAACGTGCTCTTGACT+GGG | 0.392027 | 4.2:-13248797 | MS.gene071711:CDS |
| CGTGCTCTTGACTGGGGTCC+AGG | 0.395326 | 4.2:-13248790 | MS.gene071711:CDS |
| ACAGCGCCGCCGTCTTCGCC+TGG | 0.403265 | 4.2:-13248852 | MS.gene071711:CDS |
| GTCACGAGCGATGGCGGCGG+CGG | 0.418277 | 4.2:+13248873 | None:intergenic |
| CGCCGCCGTCTTCGCCTGGA+AGG | 0.420226 | 4.2:-13248848 | MS.gene071711:CDS |
| TTGAGTTGAGAAAATGTTGC+AGG | 0.426919 | 4.2:+13248903 | None:intergenic |
| CTTGACTGGGGTCCAGGTGG+TGG | 0.427852 | 4.2:-13248784 | MS.gene071711:CDS |
| GAGCGATGGCGGCGGCGGCG+TGG | 0.480226 | 4.2:+13248878 | None:intergenic |
| GGCGCTGTCACGAGCGATGG+CGG | 0.513966 | 4.2:+13248867 | None:intergenic |
| GTGAGACTCTTCAAGAGTAT+TGG | 0.531338 | 4.2:-13248825 | MS.gene071711:CDS |
| GCTGTCACGAGCGATGGCGG+CGG | 0.534560 | 4.2:+13248870 | None:intergenic |
| CTTTAGGTGCTGAAGTCCGT+TGG | 0.543805 | 4.2:-13248930 | None:intergenic |
| CCATCGTCAACAATCAAGTC+AGG | 0.547026 | 4.2:+13248760 | None:intergenic |
| AGACTCTTCAAGAGTATTGG+TGG | 0.548092 | 4.2:-13248822 | MS.gene071711:CDS |
| ATCAAGTCAGGACCACCACC+TGG | 0.553340 | 4.2:+13248772 | None:intergenic |
| GGCGGCGCTGTCACGAGCGA+TGG | 0.576329 | 4.2:+13248864 | None:intergenic |
| CTCACCCTTCCAGGCGAAGA+CGG | 0.582229 | 4.2:+13248843 | None:intergenic |
| GCCGCCGTCTTCGCCTGGAA+GGG | 0.586397 | 4.2:-13248847 | MS.gene071711:CDS |
| ACCCTTCCAGGCGAAGACGG+CGG | 0.621848 | 4.2:+13248846 | None:intergenic |
| AAATGTTGCAGGAACACCAA+CGG | 0.640245 | 4.2:+13248914 | None:intergenic |
| GCTCTTGACTGGGGTCCAGG+TGG | 0.660501 | 4.2:-13248787 | MS.gene071711:CDS |
| GACTTGATTGTTGACGATGG+TGG | 0.670668 | 4.2:-13248757 | None:intergenic |
| CCTGACTTGATTGTTGACGA+TGG | 0.671777 | 4.2:-13248760 | MS.gene071711:CDS |
| ACTGAACGTGCTCTTGACTG+GGG | 0.690263 | 4.2:-13248796 | MS.gene071711:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGAGTTGAGAAAATGTTGC+AGG | + | chr4.2:13248782-13248801 | None:intergenic | 35.0% |
| AAATGTTGCAGGAACACCAA+CGG | + | chr4.2:13248771-13248790 | None:intergenic | 40.0% | |
| ! | GTGAGACTCTTCAAGAGTAT+TGG | - | chr4.2:13248857-13248876 | MS.gene071711:CDS | 40.0% |
| !! | AGACTCTTCAAGAGTATTGG+TGG | - | chr4.2:13248860-13248879 | MS.gene071711:CDS | 40.0% |
| CCATCGTCAACAATCAAGTC+AGG | + | chr4.2:13248925-13248944 | None:intergenic | 45.0% | |
| GACTTGATTGTTGACGATGG+TGG | - | chr4.2:13248925-13248944 | MS.gene071711:CDS | 45.0% | |
| TACTGAACGTGCTCTTGACT+GGG | - | chr4.2:13248885-13248904 | MS.gene071711:CDS | 45.0% | |
| ! | CCTGACTTGATTGTTGACGA+TGG | - | chr4.2:13248922-13248941 | MS.gene071711:CDS | 45.0% |
| ACTGAACGTGCTCTTGACTG+GGG | - | chr4.2:13248886-13248905 | MS.gene071711:CDS | 50.0% | |
| GTACTGAACGTGCTCTTGAC+TGG | - | chr4.2:13248884-13248903 | MS.gene071711:CDS | 50.0% | |
| TTGAAGAGTCTCACCCTTCC+AGG | + | chr4.2:13248851-13248870 | None:intergenic | 50.0% | |
| ATCAAGTCAGGACCACCACC+TGG | + | chr4.2:13248913-13248932 | None:intergenic | 55.0% | |
| CTCACCCTTCCAGGCGAAGA+CGG | + | chr4.2:13248842-13248861 | None:intergenic | 60.0% | |
| ACCCTTCCAGGCGAAGACGG+CGG | + | chr4.2:13248839-13248858 | None:intergenic | 65.0% | |
| CGTGCTCTTGACTGGGGTCC+AGG | - | chr4.2:13248892-13248911 | MS.gene071711:CDS | 65.0% | |
| CTTGACTGGGGTCCAGGTGG+TGG | - | chr4.2:13248898-13248917 | MS.gene071711:CDS | 65.0% | |
| GCTCTTGACTGGGGTCCAGG+TGG | - | chr4.2:13248895-13248914 | MS.gene071711:CDS | 65.0% | |
| GCCGCCGTCTTCGCCTGGAA+GGG | - | chr4.2:13248835-13248854 | MS.gene071711:CDS | 70.0% | |
| GGCGCTGTCACGAGCGATGG+CGG | + | chr4.2:13248818-13248837 | None:intergenic | 70.0% | |
| ! | GCTGTCACGAGCGATGGCGG+CGG | + | chr4.2:13248815-13248834 | None:intergenic | 70.0% |
| ACAGCGCCGCCGTCTTCGCC+TGG | - | chr4.2:13248830-13248849 | MS.gene071711:CDS | 75.0% | |
| CGCCGCCGTCTTCGCCTGGA+AGG | - | chr4.2:13248834-13248853 | MS.gene071711:CDS | 75.0% | |
| GGCGGCGCTGTCACGAGCGA+TGG | + | chr4.2:13248821-13248840 | None:intergenic | 75.0% | |
| ! | GTCACGAGCGATGGCGGCGG+CGG | + | chr4.2:13248812-13248831 | None:intergenic | 75.0% |
| !! | GAGCGATGGCGGCGGCGGCG+TGG | + | chr4.2:13248807-13248826 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 13248758 | 13248946 | 13248758 | ID=MS.gene071711 |
| chr4.2 | mRNA | 13248758 | 13248946 | 13248758 | ID=MS.gene071711.t1;Parent=MS.gene071711 |
| chr4.2 | exon | 13248758 | 13248946 | 13248758 | ID=MS.gene071711.t1.exon1;Parent=MS.gene071711.t1 |
| chr4.2 | CDS | 13248758 | 13248946 | 13248758 | ID=cds.MS.gene071711.t1;Parent=MS.gene071711.t1 |
| Gene Sequence |
| Protein sequence |
