Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059429.t1 | XP_003607873.1 | 87 | 718 | 29 | 4 | 23 | 676 | 1 | 718 | 0.00E+00 | 1159.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059429.t1 | Q9SY60 | 57.2 | 713 | 232 | 9 | 23 | 672 | 1 | 703 | 6.1e-209 | 728.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059429.t1 | A2Q297 | 87.0 | 718 | 29 | 4 | 23 | 676 | 1 | 718 | 0.0e+00 | 1159.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059429 | MS.gene059430 | 0.871011 | 9.45E-67 | -1.69E-46 |
| MS.gene059429 | MS.gene059432 | 0.888551 | 5.34E-73 | -1.69E-46 |
| MS.gene059429 | MS.gene059433 | 0.870171 | 1.78E-66 | -1.69E-46 |
| MS.gene059429 | MS.gene059434 | 0.891897 | 2.61E-74 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059429.t1 | MTR_4g083940 | 40.351 | 228 | 108 | 4 | 527 | 731 | 551 | 773 | 1.35e-37 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 143 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCCTTCACTTCCATTTC+AGG | 0.212758 | 4.4:-29078405 | MS.gene059429:CDS |
| TCTGAAGTACTGCTCAATTT+TGG | 0.215791 | 4.4:-29079722 | MS.gene059429:CDS |
| AAATGCTAGTGTAGAATTAA+AGG | 0.251429 | 4.4:-29078587 | MS.gene059429:CDS |
| GAGGATCTTGACTGGGATTC+TGG | 0.260547 | 4.4:-29078431 | MS.gene059429:CDS |
| ATCTCCATGAGCCCACTATT+TGG | 0.273163 | 4.4:+29078625 | None:intergenic |
| GATTCTTGATATGCACTTTA+CGG | 0.276409 | 4.4:-29075391 | MS.gene059429:CDS |
| TCGGAACAACTTGCAATATT+AGG | 0.290817 | 4.4:-29078727 | MS.gene059429:CDS |
| CAATATGCACTCAAAGTTCT+TGG | 0.302770 | 4.4:-29081908 | MS.gene059429:CDS |
| TATCTGACGAGCAAGAATTC+TGG | 0.316234 | 4.4:-29077797 | MS.gene059429:CDS |
| GTACAAACTACAGGAGATAT+TGG | 0.318472 | 4.4:-29078920 | MS.gene059429:intron |
| AGAAAACATTGATGTACTTC+TGG | 0.318742 | 4.4:-29080287 | MS.gene059429:CDS |
| ATCATCTTAGACAATGTAAA+AGG | 0.322480 | 4.4:+29079933 | None:intergenic |
| AAGCATTATTCCCCAATCTA+AGG | 0.326731 | 4.4:-29082669 | MS.gene059429:CDS |
| GTTGAAATCGCACGATTTGC+GGG | 0.327235 | 4.4:-29075369 | MS.gene059429:CDS |
| CATGTTGATAATACCGCATT+TGG | 0.334966 | 4.4:+29078673 | None:intergenic |
| TGTAATTTCAATGACAATAA+AGG | 0.337606 | 4.4:-29079904 | MS.gene059429:CDS |
| TCAATGACAATAAAGGAATC+TGG | 0.346816 | 4.4:-29079897 | MS.gene059429:CDS |
| TTCTGCTAGCATCTGCATTC+AGG | 0.349261 | 4.4:-29079749 | MS.gene059429:CDS |
| GATTCCACTTATCTAATTCA+TGG | 0.350347 | 4.4:+29080399 | None:intergenic |
| AGGAATAGCGTTTACCATAC+TGG | 0.359988 | 4.4:-29078707 | MS.gene059429:CDS |
| AATGTAAAAGGAAATGTTTC+AGG | 0.360157 | 4.4:+29079945 | None:intergenic |
| GCTCTCAACAAGCATGCTAT+TGG | 0.360734 | 4.4:+29079519 | None:intergenic |
| CAAGTTTGAAGAAGCATTAG+AGG | 0.362873 | 4.4:-29080257 | MS.gene059429:CDS |
| CTGTGAACTCTTAGATCTAA+AGG | 0.366187 | 4.4:-29081953 | MS.gene059429:CDS |
| ATGAATAATAATACCTTTGT+GGG | 0.378629 | 4.4:+29075265 | None:intergenic |
| TGGTAACAGTTCATCCAGTA+TGG | 0.380863 | 4.4:+29078693 | None:intergenic |
| AGTAAATATCTGCATTCAAT+CGG | 0.393201 | 4.4:+29078467 | None:intergenic |
| GAGAGAATCAACCTTAGATT+GGG | 0.401738 | 4.4:+29082658 | None:intergenic |
| GCAATAAACAAGCTCTTGCT+TGG | 0.407113 | 4.4:-29075112 | MS.gene059429:CDS |
| CAATAAACAAGCTCTTGCTT+GGG | 0.407426 | 4.4:-29075111 | MS.gene059429:CDS |
| GCTGTCAAGTTCTGCCATGC+TGG | 0.408259 | 4.4:+29074962 | None:intergenic |
| GAGCTTAAGAAGGCCTTAGA+TGG | 0.411077 | 4.4:-29080077 | MS.gene059429:CDS |
| CCATCCGGACCTTCTCTGCT+AGG | 0.414225 | 4.4:-29075296 | MS.gene059429:CDS |
| GGTTGAAATCGCACGATTTG+CGG | 0.414431 | 4.4:-29075370 | MS.gene059429:CDS |
| ATCTGACGAGCAAGAATTCT+GGG | 0.422635 | 4.4:-29077796 | MS.gene059429:CDS |
| ACAACTCTGTTGGTATAAAC+AGG | 0.424568 | 4.4:+29079855 | None:intergenic |
| ATAAGAATGATGACCTGAAA+TGG | 0.425331 | 4.4:+29078392 | None:intergenic |
| AGAGAGAATCAACCTTAGAT+TGG | 0.425927 | 4.4:+29082657 | None:intergenic |
| CACTTTGGAGGAAATGTACT+AGG | 0.430108 | 4.4:-29078871 | MS.gene059429:CDS |
| CTTGTAAAGGATTTAATGAC+TGG | 0.430610 | 4.4:-29080428 | MS.gene059429:CDS |
| AATGAATAATAATACCTTTG+TGG | 0.431699 | 4.4:+29075264 | None:intergenic |
| TGCCCCTAGCAGAGAAGGTC+CGG | 0.451416 | 4.4:+29075292 | None:intergenic |
| GTCTGCTCTCATAGAAAGAA+AGG | 0.459264 | 4.4:-29080156 | MS.gene059429:CDS |
| GAAAGTAAGGAACCAAATAG+TGG | 0.459390 | 4.4:-29078637 | MS.gene059429:CDS |
| ACTGCCACTTGGTTTGCATC+AGG | 0.461751 | 4.4:-29077745 | MS.gene059429:intron |
| CTGTAGAGTCCATCGTAGAC+AGG | 0.462840 | 4.4:+29075000 | None:intergenic |
| GATGCTGCAAAATGAAAGTA+AGG | 0.463937 | 4.4:-29078650 | MS.gene059429:CDS |
| CAGCATTAGTTGATGTGCTA+CGG | 0.466889 | 4.4:+29080031 | None:intergenic |
| GTGCACTTCCTGCGGATGAA+TGG | 0.467391 | 4.4:-29075156 | MS.gene059429:CDS |
| GCAAGAATTCTGGGGTGTCT+TGG | 0.467617 | 4.4:-29077787 | MS.gene059429:CDS |
| AGTGGGCTCATGGAGATTGT+AGG | 0.468495 | 4.4:-29078619 | MS.gene059429:CDS |
| AGTTCTTGACATGGCAGAAT+CGG | 0.473021 | 4.4:-29079602 | MS.gene059429:CDS |
| AGCTCAAGATATTCTTGTAA+AGG | 0.474934 | 4.4:-29080441 | MS.gene059429:CDS |
| CTATTATTGCTCGTGCCATC+CGG | 0.479454 | 4.4:-29075311 | MS.gene059429:CDS |
| CTATCAGTCTCAAACTGAAA+AGG | 0.482629 | 4.4:-29082636 | MS.gene059429:intron |
| ATCAGGATCAGACAAGTTAG+AGG | 0.482911 | 4.4:+29074938 | None:intergenic |
| TCAGGACAGAAAGAACTTGA+TGG | 0.485886 | 4.4:+29079963 | None:intergenic |
| GGCACAGCCTCTTTAAGCTC+TGG | 0.487213 | 4.4:+29078769 | None:intergenic |
| CATCCGGACCTTCTCTGCTA+GGG | 0.489589 | 4.4:-29075295 | MS.gene059429:CDS |
| GATAACAAGAGGATCTTGAC+TGG | 0.490850 | 4.4:-29078439 | MS.gene059429:CDS |
| AGCATTAGTTGATGTGCTAC+GGG | 0.495022 | 4.4:+29080032 | None:intergenic |
| AAGTTGTTCCGAGTCTGTCT+CGG | 0.501878 | 4.4:+29078738 | None:intergenic |
| GGAATAATGCTTTCGATGGA+AGG | 0.504051 | 4.4:+29082680 | None:intergenic |
| TCAGTTCCTCTACTGCCACT+TGG | 0.507196 | 4.4:-29077756 | MS.gene059429:CDS |
| TGCTTCTTCAAACTTGTGCT+CGG | 0.508758 | 4.4:+29080264 | None:intergenic |
| GTAAATATCTGCATTCAATC+GGG | 0.512134 | 4.4:+29078468 | None:intergenic |
| TTGTCATCATCGGAAGGGCC+TGG | 0.512178 | 4.4:+29078796 | None:intergenic |
| GGAACCAAATAGTGGGCTCA+TGG | 0.517547 | 4.4:-29078629 | MS.gene059429:CDS |
| GGCAGTGCTTGAAGACCAGC+TGG | 0.519514 | 4.4:-29080135 | MS.gene059429:CDS |
| TCTAAGGCCTTCTTAAGCTC+AGG | 0.525157 | 4.4:+29080080 | None:intergenic |
| TGAAATGGAAGTGAAGGCAG+TGG | 0.528128 | 4.4:+29078407 | None:intergenic |
| AATAAACAAGCTCTTGCTTG+GGG | 0.529684 | 4.4:-29075110 | MS.gene059429:CDS |
| GTGGGCTCATGGAGATTGTA+GGG | 0.536664 | 4.4:-29078618 | MS.gene059429:CDS |
| TTGGGGAATAATGCTTTCGA+TGG | 0.537651 | 4.4:+29082676 | None:intergenic |
| CAAAGTTCTTGGCTTTCTTG+AGG | 0.537754 | 4.4:-29081897 | MS.gene059429:intron |
| AGTTTCATCTATTCAAGAGA+GGG | 0.540337 | 4.4:-29078496 | MS.gene059429:CDS |
| CCAAAGTGCAAAACAGCCAT+TGG | 0.541109 | 4.4:+29078886 | None:intergenic |
| AGAATTCTGGGGTGTCTTGG+AGG | 0.545615 | 4.4:-29077784 | MS.gene059429:CDS |
| TCGACAAACCATTCATCCGC+AGG | 0.545644 | 4.4:+29075148 | None:intergenic |
| TCTAAAACTTCTTCAACAGA+AGG | 0.546578 | 4.4:+29079652 | None:intergenic |
| CTTAGATCTAAAGGATTCTG+TGG | 0.551408 | 4.4:-29081944 | MS.gene059429:CDS |
| GTTCTTATCAAAGCACTGCC+AGG | 0.551848 | 4.4:-29078814 | MS.gene059429:CDS |
| CAATCTTCCAGAGCTTAAAG+AGG | 0.552435 | 4.4:-29078776 | MS.gene059429:CDS |
| CTCTGGAAGATTGTCATCAT+CGG | 0.552997 | 4.4:+29078786 | None:intergenic |
| GTTTGTCGAAACTGCAAAGT+CGG | 0.558294 | 4.4:-29075134 | MS.gene059429:CDS |
| TTAGATGGTTTGATAAAACT+CGG | 0.561757 | 4.4:-29080062 | MS.gene059429:CDS |
| GAGTCTGTCTCGGCTCTGAA+AGG | 0.562375 | 4.4:+29078748 | None:intergenic |
| ACAACTATTTGATAAATACA+TGG | 0.563452 | 4.4:-29078839 | MS.gene059429:CDS |
| GCAGACACTGTCTCAGATGA+TGG | 0.565567 | 4.4:+29079778 | None:intergenic |
| TTTACTTGAGTGATAACAAG+AGG | 0.568352 | 4.4:-29078450 | MS.gene059429:CDS |
| CTAGTGTAGAATTAAAGGAA+TGG | 0.569164 | 4.4:-29078582 | MS.gene059429:CDS |
| TAAACAAGCTCTTGCTTGGG+GGG | 0.570322 | 4.4:-29075108 | MS.gene059429:CDS |
| GTTTATGCCCCTAGCAGAGA+AGG | 0.570883 | 4.4:+29075287 | None:intergenic |
| AAAGTAAGGAACCAAATAGT+GGG | 0.574422 | 4.4:-29078636 | MS.gene059429:CDS |
| GCAGATGCTAGCAGAACGTA+AGG | 0.576431 | 4.4:+29079756 | None:intergenic |
| TCAGGATCAGACAAGTTAGA+GGG | 0.576795 | 4.4:+29074939 | None:intergenic |
| ATCCGGACCTTCTCTGCTAG+GGG | 0.580597 | 4.4:-29075294 | MS.gene059429:CDS |
| GTAGTTGTTGAAAGCTCGCT+CGG | 0.580938 | 4.4:+29082758 | None:intergenic |
| GATTCTGTGGAGAACTTATG+TGG | 0.581709 | 4.4:-29081931 | MS.gene059429:CDS |
| TGCAAACCAAGTGGCAGTAG+AGG | 0.581969 | 4.4:+29077750 | None:intergenic |
| GTGTAGAATTAAAGGAATGG+AGG | 0.582717 | 4.4:-29078579 | MS.gene059429:CDS |
| GCTCGGCAATACCAACCAGC+TGG | 0.584269 | 4.4:+29080120 | None:intergenic |
| TCTGCCATGAATTAGATAAG+TGG | 0.584707 | 4.4:-29080403 | MS.gene059429:CDS |
| TGGTTTGATAAAACTCGGGA+AGG | 0.587347 | 4.4:-29080057 | MS.gene059429:CDS |
| GGAAGATTGTCATCATCGGA+AGG | 0.589617 | 4.4:+29078790 | None:intergenic |
| AGCTTCGAGATCACTTCTGT+CGG | 0.591992 | 4.4:-29078531 | MS.gene059429:CDS |
| TGCAATTGCAGAAAGTGAAG+AGG | 0.592066 | 4.4:+29079549 | None:intergenic |
| AGCTCAGGAAAACTAACAGA+AGG | 0.592380 | 4.4:+29080095 | None:intergenic |
| TAGATGGTTTGATAAAACTC+GGG | 0.593142 | 4.4:-29080061 | MS.gene059429:CDS |
| TGTTTCTTCCCTGTCTACGA+TGG | 0.594482 | 4.4:-29075009 | MS.gene059429:CDS |
| AAAACTAACAGAAGGTTGCT+CGG | 0.595073 | 4.4:+29080103 | None:intergenic |
| CAAATGCGGTATTATCAACA+TGG | 0.597044 | 4.4:-29078672 | MS.gene059429:CDS |
| TAGGATATCTGAAGAAGCTG+TGG | 0.601233 | 4.4:-29080504 | MS.gene059429:CDS |
| GAGTTTCATCTATTCAAGAG+AGG | 0.601341 | 4.4:-29078497 | MS.gene059429:CDS |
| ATCTGATGAACATGTCGAGA+TGG | 0.607293 | 4.4:+29075342 | None:intergenic |
| AGCCCACTGAACAACTCTGT+TGG | 0.609878 | 4.4:+29079845 | None:intergenic |
| GAAATGGAAGTGAAGGCAGT+GGG | 0.613052 | 4.4:+29078408 | None:intergenic |
| ATGACCTGAAATGGAAGTGA+AGG | 0.617824 | 4.4:+29078401 | None:intergenic |
| GGATGAACTGTTACCAAATG+CGG | 0.618227 | 4.4:-29078686 | MS.gene059429:CDS |
| TGTAGAGTCCATCGTAGACA+GGG | 0.623108 | 4.4:+29075001 | None:intergenic |
| GATATCTGAAGAAGCTGTGG+AGG | 0.624804 | 4.4:-29080501 | MS.gene059429:CDS |
| TTTCAGAGCCGAGACAGACT+CGG | 0.625276 | 4.4:-29078746 | MS.gene059429:CDS |
| ATAAACAAGCTCTTGCTTGG+GGG | 0.629135 | 4.4:-29075109 | MS.gene059429:CDS |
| GTGCTTGAAGACCAGCTGGT+TGG | 0.631740 | 4.4:-29080131 | MS.gene059429:CDS |
| CTTCTGTGAACTGAGCAAGA+TGG | 0.631859 | 4.4:-29082729 | MS.gene059429:CDS |
| ATTGGAACAGCTTACACCAA+TGG | 0.638850 | 4.4:-29078902 | MS.gene059429:CDS |
| CCTAGCAGAGAAGGTCCGGA+TGG | 0.639329 | 4.4:+29075296 | None:intergenic |
| TAACAGAGACTGTTGTCATG+TGG | 0.643087 | 4.4:-29077821 | MS.gene059429:CDS |
| GAAGATTGTCATCATCGGAA+GGG | 0.643235 | 4.4:+29078791 | None:intergenic |
| AATAGCATGCTTGTTGAGAG+CGG | 0.645600 | 4.4:-29079517 | MS.gene059429:CDS |
| GAGGCAAACTGTGGTGACAA+TGG | 0.653080 | 4.4:+29079568 | None:intergenic |
| ATAACAAGAGGATCTTGACT+GGG | 0.656455 | 4.4:-29078438 | MS.gene059429:CDS |
| TGTTGAAAGCTCGCTCGGAG+AGG | 0.665042 | 4.4:+29082763 | None:intergenic |
| AGAGAATCAACCTTAGATTG+GGG | 0.665099 | 4.4:+29082659 | None:intergenic |
| GGTTTGATAAAACTCGGGAA+GGG | 0.673563 | 4.4:-29080056 | MS.gene059429:CDS |
| TAGAAGAGTAGTTCTTGACA+TGG | 0.677134 | 4.4:-29079611 | MS.gene059429:CDS |
| CAGGATCAGACAAGTTAGAG+GGG | 0.683374 | 4.4:+29074940 | None:intergenic |
| AGTACCTGATGCAAACCAAG+TGG | 0.692309 | 4.4:+29077741 | None:intergenic |
| GAGCAAGATGGAAAGTAGCG+AGG | 0.693328 | 4.4:-29082717 | MS.gene059429:CDS |
| CTAGGGGCATAAACCCACAA+AGG | 0.695198 | 4.4:-29075278 | MS.gene059429:intron |
| TCTGACGAGCAAGAATTCTG+GGG | 0.728977 | 4.4:-29077795 | MS.gene059429:CDS |
| GATGGCACGAGCAATAATAG+CGG | 0.743151 | 4.4:+29075314 | None:intergenic |
| GAAAGTGAAGAGGCAAACTG+TGG | 0.758643 | 4.4:+29079559 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29074919 | 29082799 | 29074919 | ID=MS.gene059429 |
| chr4.4 | mRNA | 29074919 | 29082799 | 29074919 | ID=MS.gene059429.t1;Parent=MS.gene059429 |
| chr4.4 | exon | 29082637 | 29082799 | 29082637 | ID=MS.gene059429.t1.exon1;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29082637 | 29082799 | 29082637 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29081898 | 29081992 | 29081898 | ID=MS.gene059429.t1.exon2;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29081898 | 29081992 | 29081898 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29080398 | 29080523 | 29080398 | ID=MS.gene059429.t1.exon3;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29080398 | 29080523 | 29080398 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29080219 | 29080351 | 29080219 | ID=MS.gene059429.t1.exon4;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29080219 | 29080351 | 29080219 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29079857 | 29080177 | 29079857 | ID=MS.gene059429.t1.exon5;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29079857 | 29080177 | 29079857 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29079492 | 29079806 | 29079492 | ID=MS.gene059429.t1.exon6;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29079492 | 29079806 | 29079492 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29078369 | 29078929 | 29078369 | ID=MS.gene059429.t1.exon7;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29078369 | 29078929 | 29078369 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29077746 | 29077844 | 29077746 | ID=MS.gene059429.t1.exon8;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29077746 | 29077844 | 29077746 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29075279 | 29075415 | 29075279 | ID=MS.gene059429.t1.exon9;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29075279 | 29075415 | 29075279 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| chr4.4 | exon | 29074919 | 29075177 | 29074919 | ID=MS.gene059429.t1.exon10;Parent=MS.gene059429.t1 |
| chr4.4 | CDS | 29074919 | 29075177 | 29074919 | ID=cds.MS.gene059429.t1;Parent=MS.gene059429.t1 |
| Gene Sequence |
| Protein sequence |
