Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059430.t1 | XP_003607873.1 | 71.1 | 747 | 36 | 7 | 2 | 575 | 34 | 773 | 3.10E-268 | 934.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059430.t1 | Q9SY60 | 43.4 | 701 | 202 | 13 | 2 | 527 | 34 | 714 | 8.0e-132 | 472.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059430.t1 | A2Q297 | 71.1 | 747 | 36 | 7 | 2 | 575 | 34 | 773 | 2.2e-268 | 934.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059429 | MS.gene059430 | 0.871011 | 9.45E-67 | -1.69E-46 |
| MS.gene059430 | MS.gene059432 | 0.839537 | 1.49E-57 | -1.69E-46 |
| MS.gene059430 | MS.gene059434 | 0.882047 | 1.44E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCCTTCACTTCCATTTC+AGG | 0.212758 | 4.4:-29117497 | MS.gene059430:intron |
| AAATGCTAGTGTAGAATTAA+AGG | 0.251429 | 4.4:-29117681 | MS.gene059430:CDS |
| GAGGATCTTGACTGGGATTC+TGG | 0.261393 | 4.4:-29117523 | MS.gene059430:CDS |
| ATCTCCATGAGCCCACTATT+TGG | 0.273163 | 4.4:+29117719 | None:intergenic |
| CTCGAACAACTTGCAATATT+AGG | 0.313998 | 4.4:-29117821 | MS.gene059430:CDS |
| TATCTGACGAGCAAGAATTC+TGG | 0.316234 | 4.4:-29116889 | MS.gene059430:CDS |
| GTACAAACTACAGGAGATAT+TGG | 0.318472 | 4.4:-29118014 | MS.gene059430:CDS |
| AGAAAACATTGATGTACTTC+TGG | 0.318742 | 4.4:-29119381 | MS.gene059430:CDS |
| ATCATCTTAGACAATGTAAA+AGG | 0.322480 | 4.4:+29119030 | None:intergenic |
| CGAGTTTATCAAACCATCTA+AGG | 0.328028 | 4.4:+29119160 | None:intergenic |
| CATGTTGATAATACCGCATT+TGG | 0.334966 | 4.4:+29117767 | None:intergenic |
| TGTAATTTCAATGACAATAA+AGG | 0.337606 | 4.4:-29119001 | MS.gene059430:CDS |
| TCAATGACAATAAAGGAATC+TGG | 0.346816 | 4.4:-29118994 | MS.gene059430:CDS |
| GATTCCACTTATCTAATTCA+TGG | 0.350347 | 4.4:+29119492 | None:intergenic |
| AGGAATAGCGTTTACCATAC+TGG | 0.359988 | 4.4:-29117801 | MS.gene059430:CDS |
| AATGTAAAAGGAAATGTTTC+AGG | 0.360157 | 4.4:+29119042 | None:intergenic |
| GCTCTCAACAAGCATGCTAT+TGG | 0.360734 | 4.4:+29118612 | None:intergenic |
| CAAGTTTGAAGAAGCATTAG+AGG | 0.362873 | 4.4:-29119351 | MS.gene059430:CDS |
| AATATGCCACTCAAAGTTCT+TGG | 0.368336 | 4.4:-29120997 | MS.gene059430:CDS |
| TGGTAACAGTTCATCCAGTA+TGG | 0.380863 | 4.4:+29117787 | None:intergenic |
| AGTAAATATCTGCATTCAAT+CGG | 0.393201 | 4.4:+29117560 | None:intergenic |
| GCAATAAACAAGCTCTTGCT+TGG | 0.407113 | 4.4:-29114210 | MS.gene059430:CDS |
| CAATAAACAAGCTCTTGCTT+GGG | 0.407426 | 4.4:-29114209 | MS.gene059430:CDS |
| GCTGTCAAGTTCTGCCATGC+TGG | 0.408259 | 4.4:+29114059 | None:intergenic |
| GAGCTTAAGAAGGCCTTAGA+TGG | 0.411077 | 4.4:-29119173 | MS.gene059430:intron |
| TGTGAAACTCTTAGATCAAA+AGG | 0.418183 | 4.4:-29121043 | MS.gene059430:CDS |
| ATCTGACGAGCAAGAATTCT+GGG | 0.422635 | 4.4:-29116888 | MS.gene059430:CDS |
| ACAACTCTGTTGGTATAAAC+AGG | 0.424568 | 4.4:+29118952 | None:intergenic |
| ATAAGAATGATGACCTGAAA+TGG | 0.425331 | 4.4:+29117484 | None:intergenic |
| CACTTTGGAGGAAATGTACT+AGG | 0.430108 | 4.4:-29117964 | MS.gene059430:CDS |
| CTTGTAAAGGATTTAATGAC+TGG | 0.430610 | 4.4:-29119521 | MS.gene059430:CDS |
| TGTAGAGTCCATCGGTAGAC+AGG | 0.436023 | 4.4:+29114097 | None:intergenic |
| GAAAGTAAGGAACCAAATAG+TGG | 0.459390 | 4.4:-29117731 | MS.gene059430:CDS |
| ACTGCCACTTGGTTTGCATC+AGG | 0.461751 | 4.4:-29116837 | MS.gene059430:CDS |
| GATGCTGCAAAATGAAAGTA+AGG | 0.463937 | 4.4:-29117744 | MS.gene059430:CDS |
| CAGCATTAGTTGATGTGCTA+CGG | 0.466889 | 4.4:+29119128 | None:intergenic |
| GTGCACTTCCTGCGGATGAA+TGG | 0.467391 | 4.4:-29114254 | MS.gene059430:CDS |
| GCAAGAATTCTGGGGTGTCT+TGG | 0.467617 | 4.4:-29116879 | MS.gene059430:CDS |
| AGTGGGCTCATGGAGATTGT+AGG | 0.468495 | 4.4:-29117713 | MS.gene059430:CDS |
| AGTTCTTGACATGGCAGAAT+CGG | 0.473021 | 4.4:-29118695 | MS.gene059430:CDS |
| AGCTCAAGATATTCTTGTAA+AGG | 0.474934 | 4.4:-29119534 | MS.gene059430:CDS |
| ATCAGGATCAGACAAGTTAG+AGG | 0.482911 | 4.4:+29114035 | None:intergenic |
| TCAGGACAGAAAGAACTTGA+TGG | 0.485886 | 4.4:+29119060 | None:intergenic |
| AGCATTAGTTGATGTGCTAC+GGG | 0.495022 | 4.4:+29119129 | None:intergenic |
| CTTAGATGGTTTGATAAACT+CGG | 0.503848 | 4.4:-29119159 | MS.gene059430:CDS |
| TCAGTTCCTCTACTGCCACT+TGG | 0.507196 | 4.4:-29116848 | MS.gene059430:CDS |
| TGCTTCTTCAAACTTGTGCT+CGG | 0.508758 | 4.4:+29119358 | None:intergenic |
| GTGGCTGGAGATGTGTTACT+TGG | 0.511571 | 4.4:-29116971 | MS.gene059430:CDS |
| GTAAATATCTGCATTCAATC+GGG | 0.512134 | 4.4:+29117561 | None:intergenic |
| TTGTCATCATCGGAAGGGCC+TGG | 0.512178 | 4.4:+29117889 | None:intergenic |
| AGTTGTTCAGTGGGCTGAGT+GGG | 0.512900 | 4.4:-29118935 | MS.gene059430:CDS |
| GGAACCAAATAGTGGGCTCA+TGG | 0.517547 | 4.4:-29117723 | MS.gene059430:CDS |
| AATAAACAAGCTCTTGCTTG+GGG | 0.523439 | 4.4:-29114208 | MS.gene059430:CDS |
| AGTGTATTTGTACAAACTAC+AGG | 0.523999 | 4.4:-29118023 | MS.gene059430:CDS |
| TGAAATGGAAGTGAAGGCAG+TGG | 0.528128 | 4.4:+29117499 | None:intergenic |
| CCAAAGTGCAAAACAGCCAT+TGG | 0.531437 | 4.4:+29117979 | None:intergenic |
| CAACAATTAGCAATTGTGGC+TGG | 0.535383 | 4.4:-29116986 | MS.gene059430:CDS |
| GTGGGCTCATGGAGATTGTA+GGG | 0.536664 | 4.4:-29117712 | MS.gene059430:CDS |
| CAAAGTTCTTGGCTTTCTTG+AGG | 0.537754 | 4.4:-29120986 | MS.gene059430:intron |
| GTTTCTTCCCTGTCTACCGA+TGG | 0.539961 | 4.4:-29114105 | MS.gene059430:CDS |
| AATCTTTGCTTCTGCCAGCA+TGG | 0.540071 | 4.4:-29114073 | MS.gene059430:CDS |
| AGAATTCTGGGGTGTCTTGG+AGG | 0.545615 | 4.4:-29116876 | MS.gene059430:CDS |
| TCGACAAACCATTCATCCGC+AGG | 0.545644 | 4.4:+29114246 | None:intergenic |
| ATAACAAAGAGGATCTTGAC+TGG | 0.548772 | 4.4:-29117531 | MS.gene059430:CDS |
| CTTAGATCAAAAGGATTCTG+TGG | 0.551829 | 4.4:-29121034 | MS.gene059430:CDS |
| GTTCTTATCAAAGCACTGCC+AGG | 0.551848 | 4.4:-29117907 | MS.gene059430:CDS |
| CTCTGGAAGATTGTCATCAT+CGG | 0.552997 | 4.4:+29117879 | None:intergenic |
| GCTGCAACAATTAGCAATTG+TGG | 0.553957 | 4.4:-29116990 | MS.gene059430:CDS |
| TAAACAAGCTCTTGCTTGGG+GGG | 0.555964 | 4.4:-29114206 | MS.gene059430:CDS |
| GTTTGTCGAAACTGCAAAGT+CGG | 0.558294 | 4.4:-29114232 | MS.gene059430:CDS |
| ACAACTATTTGATAAATACA+TGG | 0.563452 | 4.4:-29117932 | MS.gene059430:CDS |
| ATGGTTTGATAAACTCGGGA+AGG | 0.564158 | 4.4:-29119154 | MS.gene059430:CDS |
| GCAGACACTGTCTCAGATGA+TGG | 0.565567 | 4.4:+29118874 | None:intergenic |
| CTAGTGTAGAATTAAAGGAA+TGG | 0.569164 | 4.4:-29117676 | MS.gene059430:CDS |
| AAAGTAAGGAACCAAATAGT+GGG | 0.574422 | 4.4:-29117730 | MS.gene059430:CDS |
| TCAGGATCAGACAAGTTAGA+GGG | 0.576795 | 4.4:+29114036 | None:intergenic |
| TTAGATGGTTTGATAAACTC+GGG | 0.579715 | 4.4:-29119158 | MS.gene059430:CDS |
| GATTCTGTGGAGAACTTATG+TGG | 0.581709 | 4.4:-29121021 | MS.gene059430:CDS |
| TGCAAACCAAGTGGCAGTAG+AGG | 0.581969 | 4.4:+29116842 | None:intergenic |
| GTGTAGAATTAAAGGAATGG+AGG | 0.582717 | 4.4:-29117673 | MS.gene059430:CDS |
| TTGGAACAGCTTACAACCAA+TGG | 0.583816 | 4.4:-29117995 | MS.gene059430:CDS |
| TCTGCCATGAATTAGATAAG+TGG | 0.584707 | 4.4:-29119496 | MS.gene059430:CDS |
| GGAAGATTGTCATCATCGGA+AGG | 0.589617 | 4.4:+29117883 | None:intergenic |
| AGCTTCGAGATCACTTCTGT+CGG | 0.591992 | 4.4:-29117625 | MS.gene059430:intron |
| TGCAATTGCAGAAAGTGAAG+AGG | 0.592066 | 4.4:+29118642 | None:intergenic |
| CAAATGCGGTATTATCAACA+TGG | 0.597044 | 4.4:-29117766 | MS.gene059430:CDS |
| ATAAACAAGCTCTTGCTTGG+GGG | 0.600434 | 4.4:-29114207 | MS.gene059430:CDS |
| TACCAACAGAGTTGTTCAGT+GGG | 0.602592 | 4.4:-29118944 | MS.gene059430:CDS |
| AAACAAGCTCTTGCTTGGGG+GGG | 0.604049 | 4.4:-29114205 | MS.gene059430:CDS |
| TTACTTGAGTGATAACAAAG+AGG | 0.604703 | 4.4:-29117542 | MS.gene059430:CDS |
| TGGTTTGATAAACTCGGGAA+GGG | 0.605676 | 4.4:-29119153 | MS.gene059430:CDS |
| AGCCCACTGAACAACTCTGT+TGG | 0.609878 | 4.4:+29118942 | None:intergenic |
| GAAATGGAAGTGAAGGCAGT+GGG | 0.613052 | 4.4:+29117500 | None:intergenic |
| ATGACCTGAAATGGAAGTGA+AGG | 0.617824 | 4.4:+29117493 | None:intergenic |
| GGATGAACTGTTACCAAATG+CGG | 0.618227 | 4.4:-29117780 | MS.gene059430:CDS |
| AAAGATTCTGTAGAGTCCAT+CGG | 0.619657 | 4.4:+29114089 | None:intergenic |
| GTAGAGTCCATCGGTAGACA+GGG | 0.629667 | 4.4:+29114098 | None:intergenic |
| TAACAGAGACTGTTGTCATG+TGG | 0.643087 | 4.4:-29116913 | MS.gene059430:CDS |
| GAAGATTGTCATCATCGGAA+GGG | 0.643235 | 4.4:+29117884 | None:intergenic |
| AATAGCATGCTTGTTGAGAG+CGG | 0.645600 | 4.4:-29118610 | MS.gene059430:CDS |
| AGAAAGCCAAGAACTTTGAG+TGG | 0.647937 | 4.4:+29120991 | None:intergenic |
| ATACCAACAGAGTTGTTCAG+TGG | 0.648569 | 4.4:-29118945 | MS.gene059430:CDS |
| TGGCTGGAGATGTGTTACTT+GGG | 0.652414 | 4.4:-29116970 | MS.gene059430:CDS |
| GAGGCAAACTGTGGTGACAA+TGG | 0.653080 | 4.4:+29118661 | None:intergenic |
| TAACAAAGAGGATCTTGACT+GGG | 0.655528 | 4.4:-29117530 | MS.gene059430:CDS |
| GAGTTGTTCAGTGGGCTGAG+TGG | 0.658596 | 4.4:-29118936 | MS.gene059430:CDS |
| TAGAAGAGTAGTTCTTGACA+TGG | 0.677134 | 4.4:-29118704 | MS.gene059430:CDS |
| CAGGATCAGACAAGTTAGAG+GGG | 0.683374 | 4.4:+29114037 | None:intergenic |
| AGTACCTGATGCAAACCAAG+TGG | 0.692309 | 4.4:+29116833 | None:intergenic |
| TCTGACGAGCAAGAATTCTG+GGG | 0.728977 | 4.4:-29116887 | MS.gene059430:CDS |
| GAAAGTGAAGAGGCAAACTG+TGG | 0.758643 | 4.4:+29118652 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29114029 | 29121083 | 29114029 | ID=MS.gene059430 |
| chr4.4 | mRNA | 29114029 | 29121083 | 29114029 | ID=MS.gene059430.t1;Parent=MS.gene059430 |
| chr4.4 | exon | 29120987 | 29121083 | 29120987 | ID=MS.gene059430.t1.exon1;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29120987 | 29121083 | 29120987 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29119321 | 29119564 | 29119321 | ID=MS.gene059430.t1.exon2;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29119321 | 29119564 | 29119321 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29118868 | 29119186 | 29118868 | ID=MS.gene059430.t1.exon3;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29118868 | 29119186 | 29118868 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29118585 | 29118732 | 29118585 | ID=MS.gene059430.t1.exon4;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29118585 | 29118732 | 29118585 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29117880 | 29118043 | 29117880 | ID=MS.gene059430.t1.exon5;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29117880 | 29118043 | 29117880 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29117626 | 29117844 | 29117626 | ID=MS.gene059430.t1.exon6;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29117626 | 29117844 | 29117626 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29117498 | 29117594 | 29117498 | ID=MS.gene059430.t1.exon7;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29117498 | 29117594 | 29117498 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29116833 | 29117026 | 29116833 | ID=MS.gene059430.t1.exon8;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29116833 | 29117026 | 29116833 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| chr4.4 | exon | 29114029 | 29114275 | 29114029 | ID=MS.gene059430.t1.exon9;Parent=MS.gene059430.t1 |
| chr4.4 | CDS | 29114029 | 29114275 | 29114029 | ID=cds.MS.gene059430.t1;Parent=MS.gene059430.t1 |
| Gene Sequence |
| Protein sequence |
