Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059432.t1 | XP_003607873.1 | 99.1 | 662 | 6 | 0 | 1 | 662 | 1 | 662 | 0.00E+00 | 1270.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059432.t1 | Q9SY60 | 64.0 | 669 | 216 | 8 | 1 | 662 | 1 | 651 | 1.0e-226 | 787.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059432.t1 | A2Q297 | 99.1 | 662 | 6 | 0 | 1 | 662 | 1 | 662 | 0.0e+00 | 1270.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059429 | MS.gene059432 | 0.888551 | 5.34E-73 | -1.69E-46 |
| MS.gene059430 | MS.gene059432 | 0.839537 | 1.49E-57 | -1.69E-46 |
| MS.gene059432 | MS.gene059433 | 0.877251 | 7.30E-69 | -1.69E-46 |
| MS.gene059432 | MS.gene059434 | 0.912686 | 1.46E-83 | -1.69E-46 |
| MS.gene059432 | MS.gene56433 | 0.81122 | 7.81E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene71846 | MS.gene059432 | PPI |
| MS.gene76287 | MS.gene059432 | PPI |
| MS.gene065117 | MS.gene059432 | PPI |
| MS.gene27144 | MS.gene059432 | PPI |
| MS.gene059432 | MS.gene93470 | PPI |
| MS.gene84797 | MS.gene059432 | PPI |
| MS.gene029389 | MS.gene059432 | PPI |
| MS.gene021190 | MS.gene059432 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059432.t1 | MTR_4g083940 | 99.094 | 662 | 6 | 0 | 1 | 662 | 1 | 662 | 0.0 | 1338 |
| MS.gene059432.t1 | MTR_4g005930 | 34.735 | 642 | 395 | 10 | 28 | 658 | 49 | 677 | 1.71e-106 | 340 |
| MS.gene059432.t1 | MTR_6g045363 | 34.039 | 661 | 395 | 16 | 15 | 655 | 44 | 683 | 3.08e-99 | 321 |
| MS.gene059432.t1 | MTR_1g111120 | 33.835 | 665 | 393 | 15 | 15 | 655 | 40 | 681 | 8.36e-95 | 309 |
| MS.gene059432.t1 | MTR_1g069620 | 27.846 | 650 | 422 | 16 | 27 | 662 | 60 | 676 | 1.61e-50 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059432.t1 | AT1G10180 | 63.976 | 669 | 216 | 8 | 1 | 662 | 1 | 651 | 0.0 | 830 |
| MS.gene059432.t1 | AT5G49830 | 35.276 | 652 | 389 | 11 | 22 | 658 | 34 | 667 | 2.03e-104 | 335 |
| MS.gene059432.t1 | AT1G10385 | 33.742 | 652 | 404 | 11 | 18 | 658 | 39 | 673 | 3.22e-103 | 332 |
| MS.gene059432.t1 | AT5G49830 | 35.322 | 637 | 382 | 10 | 34 | 658 | 80 | 698 | 1.07e-102 | 331 |
| MS.gene059432.t1 | AT5G49830 | 35.322 | 637 | 382 | 10 | 34 | 658 | 111 | 729 | 8.26e-102 | 329 |
Find 128 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCCTTCACTTCCATTTC+AGG | 0.212758 | 4.4:-29131978 | MS.gene059432:intron |
| TCTGAAGTACTGCTCAATTT+TGG | 0.215791 | 4.4:-29133296 | MS.gene059432:CDS |
| AAATGCTAGTGTAGAATTAA+AGG | 0.251429 | 4.4:-29132161 | MS.gene059432:CDS |
| GAGGATCTTGACTGGGATTC+TGG | 0.261393 | 4.4:-29132004 | MS.gene059432:CDS |
| ATCTCCATGAGCCCACTATT+TGG | 0.273163 | 4.4:+29132199 | None:intergenic |
| GATTCTTGATATGCACTTTA+CGG | 0.276409 | 4.4:-29128964 | MS.gene059432:CDS |
| TCGGAACAACTTGCAATATT+AGG | 0.290817 | 4.4:-29132301 | MS.gene059432:CDS |
| CAATATGCACTCAAAGTTCT+TGG | 0.302770 | 4.4:-29135482 | MS.gene059432:CDS |
| TATCTGACGAGCAAGAATTC+TGG | 0.316234 | 4.4:-29131368 | MS.gene059432:CDS |
| GTACAAACTACAGGAGATAT+TGG | 0.318472 | 4.4:-29132494 | MS.gene059432:intron |
| AGAAAACATTGATGTACTTC+TGG | 0.318742 | 4.4:-29133863 | MS.gene059432:CDS |
| ATCATCTTAGACAATGTAAA+AGG | 0.322480 | 4.4:+29133508 | None:intergenic |
| AAGCATTATTCCCCAATCTA+AGG | 0.326731 | 4.4:-29136245 | MS.gene059432:CDS |
| CATGTTGATAATACCGCATT+TGG | 0.334966 | 4.4:+29132247 | None:intergenic |
| TGTAATTTCAATGACAATAA+AGG | 0.337606 | 4.4:-29133479 | MS.gene059432:CDS |
| TCAATGACAATAAAGGAATC+TGG | 0.346816 | 4.4:-29133472 | MS.gene059432:CDS |
| TTCTGCTAGCATCTGCATTC+AGG | 0.349261 | 4.4:-29133323 | MS.gene059432:CDS |
| GATTCCACTTATCTAATTCA+TGG | 0.350347 | 4.4:+29133974 | None:intergenic |
| AGGAATAGCGTTTACCATAC+TGG | 0.359988 | 4.4:-29132281 | MS.gene059432:CDS |
| AATGTAAAAGGAAATGTTTC+AGG | 0.360157 | 4.4:+29133520 | None:intergenic |
| GCTCTCAACAAGCATGCTAT+TGG | 0.360734 | 4.4:+29133093 | None:intergenic |
| CAAGTTTGAAGAAGCATTAG+AGG | 0.362873 | 4.4:-29133833 | MS.gene059432:CDS |
| CTGTGAACTCTTAGATCTAA+AGG | 0.366187 | 4.4:-29135527 | MS.gene059432:CDS |
| TTGAAATCGCACCGATTTGC+GGG | 0.377140 | 4.4:-29128941 | MS.gene059432:CDS |
| TGGTAACAGTTCATCCAGTA+TGG | 0.380863 | 4.4:+29132267 | None:intergenic |
| TCTGCAGAGTTGAAAGTCTC+AGG | 0.392765 | 4.4:-29133787 | MS.gene059432:CDS |
| AGTAAATATCTGCATTCAAT+CGG | 0.393201 | 4.4:+29132041 | None:intergenic |
| GAGAGAATCAACCTTAGATT+GGG | 0.401738 | 4.4:+29136234 | None:intergenic |
| GAGCTTAAGAAGGCCTTAGA+TGG | 0.411077 | 4.4:-29133652 | MS.gene059432:CDS |
| ATCTGACGAGCAAGAATTCT+GGG | 0.422635 | 4.4:-29131367 | MS.gene059432:CDS |
| ACAACTCTGTTGGTATAAAC+AGG | 0.424568 | 4.4:+29133430 | None:intergenic |
| ATAAGAATGATGACCTGAAA+TGG | 0.425331 | 4.4:+29131965 | None:intergenic |
| AGAGAGAATCAACCTTAGAT+TGG | 0.425927 | 4.4:+29136233 | None:intergenic |
| CACTTTGGAGGAAATGTACT+AGG | 0.430108 | 4.4:-29132445 | MS.gene059432:CDS |
| CTTGTAAAGGATTTAATGAC+TGG | 0.430610 | 4.4:-29134003 | MS.gene059432:CDS |
| GTCTGCTCTCATAGAAAGAA+AGG | 0.459264 | 4.4:-29133731 | MS.gene059432:CDS |
| GAAAGTAAGGAACCAAATAG+TGG | 0.459390 | 4.4:-29132211 | MS.gene059432:CDS |
| ACTGCCACTTGGTTTGCATC+AGG | 0.461751 | 4.4:-29131316 | MS.gene059432:intron |
| GATGCTGCAAAATGAAAGTA+AGG | 0.463937 | 4.4:-29132224 | MS.gene059432:CDS |
| CAGCATTAGTTGATGTGCTA+CGG | 0.466889 | 4.4:+29133606 | None:intergenic |
| GCAAGAATTCTGGGGTGTCT+TGG | 0.467617 | 4.4:-29131358 | MS.gene059432:CDS |
| AGTGGGCTCATGGAGATTGT+AGG | 0.468495 | 4.4:-29132193 | MS.gene059432:CDS |
| AGTTCTTGACATGGCAGAAT+CGG | 0.473021 | 4.4:-29133176 | MS.gene059432:CDS |
| AGCTCAAGATATTCTTGTAA+AGG | 0.474934 | 4.4:-29134016 | MS.gene059432:CDS |
| CTATCAGTCTCAAACTGAAA+AGG | 0.482629 | 4.4:-29136212 | MS.gene059432:intron |
| TCAGGACAGAAAGAACTTGA+TGG | 0.485886 | 4.4:+29133538 | None:intergenic |
| GGCACAGCCTCTTTAAGCTC+TGG | 0.487213 | 4.4:+29132343 | None:intergenic |
| GGGAATAACTCTTCAGATGA+AGG | 0.492767 | 4.4:-29133766 | MS.gene059432:CDS |
| AGCATTAGTTGATGTGCTAC+GGG | 0.495022 | 4.4:+29133607 | None:intergenic |
| AAGTTGTTCCGAGTCTGTCT+CGG | 0.501878 | 4.4:+29132312 | None:intergenic |
| GGAATAATGCTTTCGATGGA+AGG | 0.504051 | 4.4:+29136256 | None:intergenic |
| GAGATGGATATCCCGCAAAT+CGG | 0.506881 | 4.4:+29128930 | None:intergenic |
| TCAGTTCCTCTACTGCCACT+TGG | 0.507196 | 4.4:-29131327 | MS.gene059432:CDS |
| GTTGAAATCGCACCGATTTG+CGG | 0.507290 | 4.4:-29128942 | MS.gene059432:CDS |
| TGCTTCTTCAAACTTGTGCT+CGG | 0.508758 | 4.4:+29133840 | None:intergenic |
| GTGGCTGGAGATGTGTTACT+TGG | 0.511571 | 4.4:-29131450 | MS.gene059432:CDS |
| GTAAATATCTGCATTCAATC+GGG | 0.512134 | 4.4:+29132042 | None:intergenic |
| TTGTCATCATCGGAAGGGCC+TGG | 0.512178 | 4.4:+29132370 | None:intergenic |
| AGTTGTTCAGTGGGCTGAGT+GGG | 0.512900 | 4.4:-29133413 | MS.gene059432:CDS |
| GGAACCAAATAGTGGGCTCA+TGG | 0.517547 | 4.4:-29132203 | MS.gene059432:CDS |
| GGCAGTGCTTGAAGACCAGC+TGG | 0.519514 | 4.4:-29133710 | MS.gene059432:CDS |
| TCTAAGGCCTTCTTAAGCTC+AGG | 0.525157 | 4.4:+29133655 | None:intergenic |
| TGAAATGGAAGTGAAGGCAG+TGG | 0.528128 | 4.4:+29131980 | None:intergenic |
| CAACAATTAGCAATTGTGGC+TGG | 0.535383 | 4.4:-29131465 | MS.gene059432:CDS |
| GTGGGCTCATGGAGATTGTA+GGG | 0.536664 | 4.4:-29132192 | MS.gene059432:CDS |
| TTGGGGAATAATGCTTTCGA+TGG | 0.537651 | 4.4:+29136252 | None:intergenic |
| CAAAGTTCTTGGCTTTCTTG+AGG | 0.537754 | 4.4:-29135471 | MS.gene059432:intron |
| AGTTTCATCTATTCAAGAGA+GGG | 0.540337 | 4.4:-29132070 | MS.gene059432:CDS |
| CCAAAGTGCAAAACAGCCAT+TGG | 0.541109 | 4.4:+29132460 | None:intergenic |
| AGAATTCTGGGGTGTCTTGG+AGG | 0.545615 | 4.4:-29131355 | MS.gene059432:CDS |
| TCTAAAACTTCTTCAACAGA+AGG | 0.546578 | 4.4:+29133226 | None:intergenic |
| ATAACAAAGAGGATCTTGAC+TGG | 0.548772 | 4.4:-29132012 | MS.gene059432:CDS |
| CTTAGATCTAAAGGATTCTG+TGG | 0.551408 | 4.4:-29135518 | MS.gene059432:CDS |
| GTTCTTATCAAAGCACTGCC+AGG | 0.551848 | 4.4:-29132388 | MS.gene059432:CDS |
| CAATCTTCCAGAGCTTAAAG+AGG | 0.552435 | 4.4:-29132350 | MS.gene059432:CDS |
| CTCTGGAAGATTGTCATCAT+CGG | 0.552997 | 4.4:+29132360 | None:intergenic |
| GCTGCAACAATTAGCAATTG+TGG | 0.553957 | 4.4:-29131469 | MS.gene059432:CDS |
| TTAGATGGTTTGATAAAACT+CGG | 0.561757 | 4.4:-29133637 | MS.gene059432:CDS |
| GAGTCTGTCTCGGCTCTGAA+AGG | 0.562375 | 4.4:+29132322 | None:intergenic |
| ACAACTATTTGATAAATACA+TGG | 0.563452 | 4.4:-29132413 | MS.gene059432:CDS |
| GCAGACACTGTCTCAGATGA+TGG | 0.565567 | 4.4:+29133352 | None:intergenic |
| CTAGTGTAGAATTAAAGGAA+TGG | 0.569164 | 4.4:-29132156 | MS.gene059432:CDS |
| AAAGTAAGGAACCAAATAGT+GGG | 0.574422 | 4.4:-29132210 | MS.gene059432:CDS |
| GCAGATGCTAGCAGAACGTA+AGG | 0.576431 | 4.4:+29133330 | None:intergenic |
| GATTCTGTGGAGAACTTATG+TGG | 0.581709 | 4.4:-29135505 | MS.gene059432:CDS |
| TGCAAACCAAGTGGCAGTAG+AGG | 0.581969 | 4.4:+29131321 | None:intergenic |
| GTGTAGAATTAAAGGAATGG+AGG | 0.582717 | 4.4:-29132153 | MS.gene059432:CDS |
| GCTCGGCAATACCAACCAGC+TGG | 0.584269 | 4.4:+29133695 | None:intergenic |
| TCTGCCATGAATTAGATAAG+TGG | 0.584707 | 4.4:-29133978 | MS.gene059432:CDS |
| TGGTTTGATAAAACTCGGGA+AGG | 0.587347 | 4.4:-29133632 | MS.gene059432:CDS |
| GGAAGATTGTCATCATCGGA+AGG | 0.589617 | 4.4:+29132364 | None:intergenic |
| AGCTTCGAGATCACTTCTGT+CGG | 0.591992 | 4.4:-29132105 | MS.gene059432:CDS |
| TGCAATTGCAGAAAGTGAAG+AGG | 0.592066 | 4.4:+29133123 | None:intergenic |
| AGCTCAGGAAAACTAACAGA+AGG | 0.592380 | 4.4:+29133670 | None:intergenic |
| TAGATGGTTTGATAAAACTC+GGG | 0.593142 | 4.4:-29133636 | MS.gene059432:CDS |
| AAAACTAACAGAAGGTTGCT+CGG | 0.595073 | 4.4:+29133678 | None:intergenic |
| CAAATGCGGTATTATCAACA+TGG | 0.597044 | 4.4:-29132246 | MS.gene059432:CDS |
| CTGCAGAGTTGAAAGTCTCA+GGG | 0.600112 | 4.4:-29133786 | MS.gene059432:CDS |
| TAGGATATCTGAAGAAGCTG+TGG | 0.601233 | 4.4:-29134079 | MS.gene059432:CDS |
| GAGTTTCATCTATTCAAGAG+AGG | 0.601341 | 4.4:-29132071 | MS.gene059432:CDS |
| TACCAACAGAGTTGTTCAGT+GGG | 0.602592 | 4.4:-29133422 | MS.gene059432:CDS |
| TTACTTGAGTGATAACAAAG+AGG | 0.604703 | 4.4:-29132023 | MS.gene059432:CDS |
| ATCTGATGAACATGTCGAGA+TGG | 0.607293 | 4.4:+29128914 | None:intergenic |
| AGCCCACTGAACAACTCTGT+TGG | 0.609878 | 4.4:+29133420 | None:intergenic |
| GAAATGGAAGTGAAGGCAGT+GGG | 0.613052 | 4.4:+29131981 | None:intergenic |
| ATGACCTGAAATGGAAGTGA+AGG | 0.617824 | 4.4:+29131974 | None:intergenic |
| GGATGAACTGTTACCAAATG+CGG | 0.618227 | 4.4:-29132260 | MS.gene059432:CDS |
| GATATCTGAAGAAGCTGTGG+AGG | 0.624804 | 4.4:-29134076 | MS.gene059432:CDS |
| TTTCAGAGCCGAGACAGACT+CGG | 0.625276 | 4.4:-29132320 | MS.gene059432:CDS |
| GTGCTTGAAGACCAGCTGGT+TGG | 0.631740 | 4.4:-29133706 | MS.gene059432:CDS |
| ATTGGAACAGCTTACACCAA+TGG | 0.638850 | 4.4:-29132476 | MS.gene059432:CDS |
| TAACAGAGACTGTTGTCATG+TGG | 0.643087 | 4.4:-29131392 | MS.gene059432:CDS |
| GAAGATTGTCATCATCGGAA+GGG | 0.643235 | 4.4:+29132365 | None:intergenic |
| AATAGCATGCTTGTTGAGAG+CGG | 0.645600 | 4.4:-29133091 | MS.gene059432:CDS |
| ATACCAACAGAGTTGTTCAG+TGG | 0.648569 | 4.4:-29133423 | MS.gene059432:CDS |
| TGGCTGGAGATGTGTTACTT+GGG | 0.652414 | 4.4:-29131449 | MS.gene059432:CDS |
| GAGGCAAACTGTGGTGACAA+TGG | 0.653080 | 4.4:+29133142 | None:intergenic |
| TAACAAAGAGGATCTTGACT+GGG | 0.655528 | 4.4:-29132011 | MS.gene059432:CDS |
| GAGTTGTTCAGTGGGCTGAG+TGG | 0.658596 | 4.4:-29133414 | MS.gene059432:CDS |
| AGAGAATCAACCTTAGATTG+GGG | 0.665099 | 4.4:+29136235 | None:intergenic |
| GGTTTGATAAAACTCGGGAA+GGG | 0.673563 | 4.4:-29133631 | MS.gene059432:CDS |
| TAGAAGAGTAGTTCTTGACA+TGG | 0.677134 | 4.4:-29133185 | MS.gene059432:CDS |
| AGTACCTGATGCAAACCAAG+TGG | 0.692309 | 4.4:+29131312 | None:intergenic |
| GAGCAAGATGGAAAGTAGCG+AGG | 0.693328 | 4.4:-29136293 | None:intergenic |
| TCTGACGAGCAAGAATTCTG+GGG | 0.728977 | 4.4:-29131366 | MS.gene059432:CDS |
| GATGGCACGAGCAATAATAG+CGG | 0.743151 | 4.4:+29128886 | None:intergenic |
| GAAAGTGAAGAGGCAAACTG+TGG | 0.758643 | 4.4:+29133133 | None:intergenic |
| TATGAGAGCAGACTTATACG+AGG | 0.759611 | 4.4:+29133741 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29128894 | 29136308 | 29128894 | ID=MS.gene059432 |
| chr4.4 | mRNA | 29128894 | 29136308 | 29128894 | ID=MS.gene059432.t1;Parent=MS.gene059432 |
| chr4.4 | exon | 29136213 | 29136308 | 29136213 | ID=MS.gene059432.t1.exon1;Parent=MS.gene059432.t1 |
| chr4.4 | CDS | 29136213 | 29136308 | 29136213 | ID=cds.MS.gene059432.t1;Parent=MS.gene059432.t1 |
| chr4.4 | exon | 29135472 | 29135566 | 29135472 | ID=MS.gene059432.t1.exon2;Parent=MS.gene059432.t1 |
| chr4.4 | CDS | 29135472 | 29135566 | 29135472 | ID=cds.MS.gene059432.t1;Parent=MS.gene059432.t1 |
| chr4.4 | exon | 29133066 | 29134098 | 29133066 | ID=MS.gene059432.t1.exon3;Parent=MS.gene059432.t1 |
| chr4.4 | CDS | 29133066 | 29134098 | 29133066 | ID=cds.MS.gene059432.t1;Parent=MS.gene059432.t1 |
| chr4.4 | exon | 29131979 | 29132503 | 29131979 | ID=MS.gene059432.t1.exon4;Parent=MS.gene059432.t1 |
| chr4.4 | CDS | 29131979 | 29132503 | 29131979 | ID=cds.MS.gene059432.t1;Parent=MS.gene059432.t1 |
| chr4.4 | exon | 29131317 | 29131505 | 29131317 | ID=MS.gene059432.t1.exon5;Parent=MS.gene059432.t1 |
| chr4.4 | CDS | 29131317 | 29131505 | 29131317 | ID=cds.MS.gene059432.t1;Parent=MS.gene059432.t1 |
| chr4.4 | exon | 29128894 | 29128988 | 29128894 | ID=MS.gene059432.t1.exon6;Parent=MS.gene059432.t1 |
| chr4.4 | CDS | 29128894 | 29128988 | 29128894 | ID=cds.MS.gene059432.t1;Parent=MS.gene059432.t1 |
| Gene Sequence |
| Protein sequence |
