Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059434.t1 | XP_003607873.1 | 75.5 | 690 | 34 | 8 | 1 | 559 | 1 | 686 | 1.40E-262 | 915.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059434.t1 | Q9SY60 | 50.6 | 680 | 210 | 11 | 1 | 559 | 1 | 675 | 5.2e-160 | 565.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059434.t1 | A2Q297 | 75.5 | 690 | 34 | 8 | 1 | 559 | 1 | 686 | 1.0e-262 | 915.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059429 | MS.gene059434 | 0.891897 | 2.61E-74 | -1.69E-46 |
| MS.gene059430 | MS.gene059434 | 0.882047 | 1.44E-70 | -1.69E-46 |
| MS.gene059432 | MS.gene059434 | 0.912686 | 1.46E-83 | -1.69E-46 |
| MS.gene059433 | MS.gene059434 | 0.816502 | 5.35E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059434.t1 | MTR_4g083940 | 76.399 | 661 | 34 | 7 | 1 | 543 | 1 | 657 | 0.0 | 950 |
| MS.gene059434.t1 | MTR_4g005930 | 28.228 | 666 | 333 | 17 | 28 | 558 | 49 | 704 | 1.28e-60 | 214 |
| MS.gene059434.t1 | MTR_6g045363 | 28.244 | 655 | 327 | 18 | 15 | 541 | 44 | 683 | 3.35e-57 | 204 |
| MS.gene059434.t1 | MTR_1g111120 | 28.439 | 647 | 338 | 16 | 15 | 541 | 40 | 681 | 6.69e-56 | 201 |
| MS.gene059434.t1 | MTR_1g069620 | 23.642 | 626 | 355 | 16 | 27 | 543 | 60 | 671 | 1.25e-27 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059434.t1 | AT1G10180 | 50.882 | 680 | 208 | 13 | 1 | 559 | 1 | 675 | 0.0 | 603 |
| MS.gene059434.t1 | AT5G49830 | 29.148 | 669 | 334 | 17 | 22 | 558 | 34 | 694 | 3.65e-61 | 215 |
| MS.gene059434.t1 | AT5G49830 | 29.052 | 654 | 327 | 16 | 34 | 558 | 80 | 725 | 1.67e-59 | 211 |
| MS.gene059434.t1 | AT5G49830 | 29.052 | 654 | 327 | 16 | 34 | 558 | 111 | 756 | 3.58e-59 | 211 |
| MS.gene059434.t1 | AT1G10385 | 29.148 | 669 | 339 | 19 | 18 | 558 | 39 | 700 | 1.04e-58 | 209 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCCTTCACTTCCATTTC+AGG | 0.212758 | 4.4:-29102750 | MS.gene059434:intron |
| TCTGAAGTACTGCTCAATTT+TGG | 0.215791 | 4.4:-29104060 | MS.gene059434:CDS |
| GAGGATCTTGACTGGGATTC+TGG | 0.261393 | 4.4:-29102776 | MS.gene059434:CDS |
| ATCTCCATGAGCCCACTATT+TGG | 0.273163 | 4.4:+29102972 | None:intergenic |
| GATTCTTGATATGCACTTTA+CGG | 0.276409 | 4.4:-29099745 | MS.gene059434:CDS |
| TTGCTTCCATCAAGTTCTTC+TGG | 0.289532 | 4.4:-29104308 | MS.gene059434:CDS |
| TCGGAACAACTTGCAATATT+AGG | 0.290817 | 4.4:-29103073 | MS.gene059434:CDS |
| CAATATGCACTCAAAGTTCT+TGG | 0.302770 | 4.4:-29106240 | MS.gene059434:CDS |
| TATCTGACGAGCAAGAATTC+TGG | 0.316234 | 4.4:-29102144 | MS.gene059434:CDS |
| GTACAAACTACAGGAGATAT+TGG | 0.318472 | 4.4:-29103263 | MS.gene059434:intron |
| ATCATCTTAGACAATGTAAA+AGG | 0.322480 | 4.4:+29104272 | None:intergenic |
| AAGCATTATTCCCCAATCTA+AGG | 0.326731 | 4.4:-29106998 | MS.gene059434:CDS |
| CATGTTGATAATACCGCATT+TGG | 0.334966 | 4.4:+29103019 | None:intergenic |
| TGTAATTTCAATGACAATAA+AGG | 0.337606 | 4.4:-29104243 | MS.gene059434:CDS |
| TCAATGACAATAAAGGAATC+TGG | 0.346816 | 4.4:-29104236 | MS.gene059434:CDS |
| TTCTGCTAGCATCTGCATTC+AGG | 0.349261 | 4.4:-29104087 | MS.gene059434:CDS |
| GATTCCACTTATCTAATTCA+TGG | 0.350347 | 4.4:+29104737 | None:intergenic |
| AGGAATAGCGTTTACCATAC+TGG | 0.359988 | 4.4:-29103053 | MS.gene059434:CDS |
| AATGTAAAAGGAAATGTTTC+AGG | 0.360157 | 4.4:+29104284 | None:intergenic |
| GCTCTCAACAAGCATGCTAT+TGG | 0.360734 | 4.4:+29103860 | None:intergenic |
| CTGTGAACTCTTAGATCTAA+AGG | 0.366187 | 4.4:-29106285 | MS.gene059434:CDS |
| TGGTAACAGTTCATCCAGTA+TGG | 0.380863 | 4.4:+29103039 | None:intergenic |
| TCTGCAGAGTTGAAAGTCTC+AGG | 0.392765 | 4.4:-29104551 | MS.gene059434:CDS |
| AGTAAATATCTGCATTCAAT+CGG | 0.393201 | 4.4:+29102813 | None:intergenic |
| GAGAGAATCAACCTTAGATT+GGG | 0.401738 | 4.4:+29106987 | None:intergenic |
| GAGCTTAAGAAGGCCTTAGA+TGG | 0.411077 | 4.4:-29104416 | MS.gene059434:CDS |
| CCATCCGGACCTTCTCTGCT+AGG | 0.414225 | 4.4:-29099649 | MS.gene059434:CDS |
| ATCTGACGAGCAAGAATTCT+GGG | 0.422635 | 4.4:-29102143 | MS.gene059434:CDS |
| ACAACTCTGTTGGTATAAAC+AGG | 0.424568 | 4.4:+29104194 | None:intergenic |
| ATAAGAATGATGACCTGAAA+TGG | 0.425331 | 4.4:+29102737 | None:intergenic |
| AGAGAGAATCAACCTTAGAT+TGG | 0.425927 | 4.4:+29106986 | None:intergenic |
| CACTTTGGAGGAAATGTACT+AGG | 0.430108 | 4.4:-29103214 | MS.gene059434:intron |
| CTTGTAAAGGATTTAATGAC+TGG | 0.430610 | 4.4:-29104766 | MS.gene059434:CDS |
| TGCCCCTAGCAGAGAAGGTC+CGG | 0.451416 | 4.4:+29099645 | None:intergenic |
| GTCTGCTCTCATAGAAAGAA+AGG | 0.459264 | 4.4:-29104495 | MS.gene059434:CDS |
| GAAAGTAAGGAACCAAATAG+TGG | 0.459390 | 4.4:-29102984 | MS.gene059434:CDS |
| CAGCATTAGTTGATGTGCTA+CGG | 0.466889 | 4.4:+29104370 | None:intergenic |
| GCAAGAATTCTGGGGTGTCT+TGG | 0.467617 | 4.4:-29102134 | MS.gene059434:CDS |
| AGCTCAAGATATTCTTGTAA+AGG | 0.474934 | 4.4:-29104779 | MS.gene059434:CDS |
| CTATTATTGCTCGTGCCATC+CGG | 0.479454 | 4.4:-29099664 | MS.gene059434:CDS |
| GGATGCTGCAAATGAAAGTA+AGG | 0.480335 | 4.4:-29102997 | MS.gene059434:CDS |
| CTATCAGTCTCAAACTGAAA+AGG | 0.482629 | 4.4:-29106965 | MS.gene059434:intron |
| CATCCGGACCTTCTCTGCTA+GGG | 0.489589 | 4.4:-29099648 | MS.gene059434:CDS |
| TCAGGACCAGAAGAACTTGA+TGG | 0.490345 | 4.4:+29104302 | None:intergenic |
| GGGAATAACTCTTCAGATGA+AGG | 0.492767 | 4.4:-29104530 | MS.gene059434:CDS |
| AGCATTAGTTGATGTGCTAC+GGG | 0.495022 | 4.4:+29104371 | None:intergenic |
| AAGTTGTTCCGAGTCTGTCT+CGG | 0.501878 | 4.4:+29103084 | None:intergenic |
| GGAATAATGCTTTCGATGGA+AGG | 0.504051 | 4.4:+29107009 | None:intergenic |
| GTGGCTGGAGATGTGTTACT+TGG | 0.511571 | 4.4:-29102226 | MS.gene059434:CDS |
| GTAAATATCTGCATTCAATC+GGG | 0.512134 | 4.4:+29102814 | None:intergenic |
| AGTTGTTCAGTGGGCTGAGT+GGG | 0.512900 | 4.4:-29104177 | MS.gene059434:CDS |
| GGAACCAAATAGTGGGCTCA+TGG | 0.517547 | 4.4:-29102976 | MS.gene059434:CDS |
| GGCAGTGCTTGAAGACCAGC+TGG | 0.519514 | 4.4:-29104474 | MS.gene059434:CDS |
| TCTAAGGCCTTCTTAAGCTC+AGG | 0.525157 | 4.4:+29104419 | None:intergenic |
| TGAAATGGAAGTGAAGGCAG+TGG | 0.528128 | 4.4:+29102752 | None:intergenic |
| CAACAATTAGCAATTGTGGC+TGG | 0.535383 | 4.4:-29102241 | MS.gene059434:CDS |
| TTGGGGAATAATGCTTTCGA+TGG | 0.537651 | 4.4:+29107005 | None:intergenic |
| CAAAGTTCTTGGCTTTCTTG+AGG | 0.537754 | 4.4:-29106229 | MS.gene059434:intron |
| AGTTTCATCTATTCAAGAGA+GGG | 0.540337 | 4.4:-29102842 | MS.gene059434:CDS |
| CCAAAGTGCAAAACAGCCAT+TGG | 0.541109 | 4.4:+29103229 | None:intergenic |
| AGAATTCTGGGGTGTCTTGG+AGG | 0.545615 | 4.4:-29102131 | MS.gene059434:CDS |
| ATAACAAAGAGGATCTTGAC+TGG | 0.548772 | 4.4:-29102784 | MS.gene059434:CDS |
| TCAGTTCCTCTACTGCCACT+TGG | 0.548855 | 4.4:-29102104 | MS.gene059434:intron |
| CTTAGATCTAAAGGATTCTG+TGG | 0.551408 | 4.4:-29106276 | MS.gene059434:CDS |
| GCTGCAACAATTAGCAATTG+TGG | 0.553957 | 4.4:-29102245 | MS.gene059434:CDS |
| GTGTAGAATTAAAGGAATGG+AGG | 0.560814 | 4.4:-29102926 | MS.gene059434:intron |
| TTAGATGGTTTGATAAAACT+CGG | 0.561757 | 4.4:-29104401 | MS.gene059434:CDS |
| GAGTCTGTCTCGGCTCTGAA+AGG | 0.562375 | 4.4:+29103094 | None:intergenic |
| GCAGACACTGTCTCAGATGA+TGG | 0.565567 | 4.4:+29104116 | None:intergenic |
| TGTAGAATTAAAGGAATGGA+GGG | 0.566500 | 4.4:-29102925 | MS.gene059434:intron |
| GTTTATGCCCCTAGCAGAGA+AGG | 0.570883 | 4.4:+29099640 | None:intergenic |
| ACAATCTCCAGAGCTTAAAG+AGG | 0.573238 | 4.4:-29103122 | MS.gene059434:intron |
| AAAGTAAGGAACCAAATAGT+GGG | 0.574422 | 4.4:-29102983 | MS.gene059434:CDS |
| GCAGATGCTAGCAGAACGTA+AGG | 0.576431 | 4.4:+29104094 | None:intergenic |
| ATCCGGACCTTCTCTGCTAG+GGG | 0.580597 | 4.4:-29099647 | None:intergenic |
| GATTCTGTGGAGAACTTATG+TGG | 0.581709 | 4.4:-29106263 | MS.gene059434:CDS |
| TGCAAACCAAGTGGCAGTAG+AGG | 0.581969 | 4.4:+29102098 | None:intergenic |
| GCTCGGCAATACCAACCAGC+TGG | 0.584269 | 4.4:+29104459 | None:intergenic |
| TCTGCCATGAATTAGATAAG+TGG | 0.584707 | 4.4:-29104741 | MS.gene059434:CDS |
| TGGTTTGATAAAACTCGGGA+AGG | 0.587347 | 4.4:-29104396 | MS.gene059434:CDS |
| AGCTTCGAGATCACTTCTGT+CGG | 0.591992 | 4.4:-29102877 | MS.gene059434:CDS |
| TGCAATTGCAGAAAGTGAAG+AGG | 0.592066 | 4.4:+29103890 | None:intergenic |
| AGCTCAGGAAAACTAACAGA+AGG | 0.592380 | 4.4:+29104434 | None:intergenic |
| TAGATGGTTTGATAAAACTC+GGG | 0.593142 | 4.4:-29104400 | MS.gene059434:CDS |
| AAAACTAACAGAAGGTTGCT+CGG | 0.595073 | 4.4:+29104442 | None:intergenic |
| CAAATGCGGTATTATCAACA+TGG | 0.597044 | 4.4:-29103018 | MS.gene059434:CDS |
| CTGCAGAGTTGAAAGTCTCA+GGG | 0.600112 | 4.4:-29104550 | MS.gene059434:CDS |
| TAGGATATCTGAAGAAGCTG+TGG | 0.601233 | 4.4:-29104842 | MS.gene059434:CDS |
| GAGTTTCATCTATTCAAGAG+AGG | 0.601341 | 4.4:-29102843 | MS.gene059434:CDS |
| TACCAACAGAGTTGTTCAGT+GGG | 0.602592 | 4.4:-29104186 | MS.gene059434:CDS |
| TTACTTGAGTGATAACAAAG+AGG | 0.604703 | 4.4:-29102795 | MS.gene059434:CDS |
| AGCCCACTGAACAACTCTGT+TGG | 0.609878 | 4.4:+29104184 | None:intergenic |
| GAAATGGAAGTGAAGGCAGT+GGG | 0.613052 | 4.4:+29102753 | None:intergenic |
| ATGACCTGAAATGGAAGTGA+AGG | 0.617824 | 4.4:+29102746 | None:intergenic |
| GGATGAACTGTTACCAAATG+CGG | 0.618227 | 4.4:-29103032 | MS.gene059434:CDS |
| GATATCTGAAGAAGCTGTGG+AGG | 0.624804 | 4.4:-29104839 | MS.gene059434:CDS |
| TTTCAGAGCCGAGACAGACT+CGG | 0.625276 | 4.4:-29103092 | MS.gene059434:CDS |
| GTGCTTGAAGACCAGCTGGT+TGG | 0.631740 | 4.4:-29104470 | MS.gene059434:CDS |
| ATTGGAACAGCTTACACCAA+TGG | 0.638850 | 4.4:-29103245 | MS.gene059434:CDS |
| CCTAGCAGAGAAGGTCCGGA+TGG | 0.639329 | 4.4:+29099649 | None:intergenic |
| TAACAGAGACTGTTGTCATG+TGG | 0.643087 | 4.4:-29102168 | MS.gene059434:CDS |
| AATAGCATGCTTGTTGAGAG+CGG | 0.645600 | 4.4:-29103858 | MS.gene059434:CDS |
| ATACCAACAGAGTTGTTCAG+TGG | 0.648569 | 4.4:-29104187 | MS.gene059434:CDS |
| TGGCTGGAGATGTGTTACTT+GGG | 0.652414 | 4.4:-29102225 | MS.gene059434:CDS |
| TAACAAAGAGGATCTTGACT+GGG | 0.655528 | 4.4:-29102783 | MS.gene059434:CDS |
| GAGTTGTTCAGTGGGCTGAG+TGG | 0.658596 | 4.4:-29104178 | MS.gene059434:CDS |
| AGAGAATCAACCTTAGATTG+GGG | 0.665099 | 4.4:+29106988 | None:intergenic |
| GGTTTGATAAAACTCGGGAA+GGG | 0.673563 | 4.4:-29104395 | MS.gene059434:CDS |
| GAGCAAGATGGAAAGTAGCG+AGG | 0.693328 | 4.4:-29107046 | None:intergenic |
| TCTGACGAGCAAGAATTCTG+GGG | 0.728977 | 4.4:-29102142 | MS.gene059434:CDS |
| GATGGCACGAGCAATAATAG+CGG | 0.743151 | 4.4:+29099667 | None:intergenic |
| TATGAGAGCAGACTTATACG+AGG | 0.759611 | 4.4:+29104505 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29099648 | 29107061 | 29099648 | ID=MS.gene059434 |
| chr4.4 | mRNA | 29099648 | 29107061 | 29099648 | ID=MS.gene059434.t1;Parent=MS.gene059434 |
| chr4.4 | exon | 29106966 | 29107061 | 29106966 | ID=MS.gene059434.t1.exon1;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29106966 | 29107061 | 29106966 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29106230 | 29106324 | 29106230 | ID=MS.gene059434.t1.exon2;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29106230 | 29106324 | 29106230 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29104672 | 29104861 | 29104672 | ID=MS.gene059434.t1.exon3;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29104672 | 29104861 | 29104672 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29104043 | 29104591 | 29104043 | ID=MS.gene059434.t1.exon4;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29104043 | 29104591 | 29104043 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29103833 | 29103916 | 29103833 | ID=MS.gene059434.t1.exon5;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29103833 | 29103916 | 29103833 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29103215 | 29103272 | 29103215 | ID=MS.gene059434.t1.exon6;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29103215 | 29103272 | 29103215 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29102971 | 29103133 | 29102971 | ID=MS.gene059434.t1.exon7;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29102971 | 29103133 | 29102971 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29102751 | 29102934 | 29102751 | ID=MS.gene059434.t1.exon8;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29102751 | 29102934 | 29102751 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29102105 | 29102281 | 29102105 | ID=MS.gene059434.t1.exon9;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29102105 | 29102281 | 29102105 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29099737 | 29099769 | 29099737 | ID=MS.gene059434.t1.exon10;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29099737 | 29099769 | 29099737 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| chr4.4 | exon | 29099648 | 29099696 | 29099648 | ID=MS.gene059434.t1.exon11;Parent=MS.gene059434.t1 |
| chr4.4 | CDS | 29099648 | 29099696 | 29099648 | ID=cds.MS.gene059434.t1;Parent=MS.gene059434.t1 |
| Gene Sequence |
| Protein sequence |
