Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059454.t1 | KEH16449.1 | 96.1 | 102 | 4 | 0 | 2 | 103 | 34 | 135 | 1.10E-46 | 195.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059454.t1 | Q6NMM1 | 71.6 | 102 | 29 | 0 | 2 | 103 | 39 | 140 | 2.4e-39 | 162.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059454.t1 | A0A072TFY1 | 96.1 | 102 | 4 | 0 | 2 | 103 | 34 | 135 | 7.8e-47 | 195.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059454 | MS.gene28918 | 0.805524 | 1.28E-49 | -1.69E-46 |
| MS.gene059454 | MS.gene73883 | 0.805557 | 1.26E-49 | -1.69E-46 |
| MS.gene059454 | MS.gene73885 | 0.812165 | 4.86E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059454.t1 | MTR_0180s0070 | 96.078 | 102 | 4 | 0 | 2 | 103 | 34 | 135 | 1.11e-64 | 192 |
| MS.gene059454.t1 | MTR_5g089500 | 84.314 | 102 | 16 | 0 | 2 | 103 | 39 | 140 | 7.92e-60 | 179 |
| MS.gene059454.t1 | MTR_7g006340 | 42.857 | 84 | 48 | 0 | 2 | 85 | 38 | 121 | 3.77e-20 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059454.t1 | AT5G01430 | 74.510 | 102 | 26 | 0 | 2 | 103 | 39 | 140 | 9.43e-55 | 167 |
| MS.gene059454.t1 | AT5G01430 | 74.510 | 102 | 26 | 0 | 2 | 103 | 39 | 140 | 9.43e-55 | 167 |
| MS.gene059454.t1 | AT3G49420 | 74.510 | 102 | 26 | 0 | 2 | 103 | 39 | 140 | 9.43e-55 | 167 |
| MS.gene059454.t1 | AT3G49420 | 74.510 | 102 | 26 | 0 | 2 | 103 | 39 | 140 | 9.43e-55 | 167 |
| MS.gene059454.t1 | AT3G49420 | 74.510 | 102 | 26 | 0 | 2 | 103 | 39 | 140 | 9.43e-55 | 167 |
| MS.gene059454.t1 | AT3G03180 | 71.569 | 102 | 29 | 0 | 2 | 103 | 39 | 140 | 5.73e-52 | 160 |
| MS.gene059454.t1 | AT3G03180 | 71.569 | 102 | 29 | 0 | 2 | 103 | 1 | 102 | 6.18e-52 | 158 |
| MS.gene059454.t1 | AT3G03180 | 70.000 | 90 | 27 | 0 | 2 | 91 | 39 | 128 | 2.83e-43 | 139 |
| MS.gene059454.t1 | AT1G05785 | 39.394 | 99 | 59 | 1 | 2 | 100 | 32 | 129 | 6.93e-20 | 78.6 |
| MS.gene059454.t1 | AT1G05785 | 39.394 | 99 | 59 | 1 | 2 | 100 | 38 | 135 | 7.63e-20 | 78.6 |
| MS.gene059454.t1 | AT1G05785 | 39.394 | 99 | 59 | 1 | 2 | 100 | 38 | 135 | 7.63e-20 | 78.6 |
| MS.gene059454.t1 | AT1G05785 | 39.394 | 99 | 59 | 1 | 2 | 100 | 38 | 135 | 7.63e-20 | 78.6 |
| MS.gene059454.t1 | AT1G05785 | 40.698 | 86 | 51 | 0 | 2 | 87 | 38 | 123 | 2.72e-19 | 77.0 |
| MS.gene059454.t1 | AT1G05785 | 40.000 | 90 | 54 | 0 | 2 | 91 | 38 | 127 | 3.92e-19 | 77.8 |
| MS.gene059454.t1 | AT1G05785 | 40.000 | 90 | 54 | 0 | 2 | 91 | 38 | 127 | 3.92e-19 | 77.8 |
Find 31 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCAGATCCTCTTTGTTTC+TGG | 0.102218 | 3.2:-35477106 | MS.gene059454:intron |
| TATATTGGGATGGCCTATTT+TGG | 0.127317 | 3.2:-35476877 | MS.gene059454:CDS |
| AGGATTCTTGATTCTTATAT+TGG | 0.190758 | 3.2:-35476892 | MS.gene059454:CDS |
| TTGCAGAGGATTCCTGTTCT+TGG | 0.230649 | 3.2:-35476708 | MS.gene059454:CDS |
| GGAATGATTGTGGAATCTTA+TGG | 0.317792 | 3.2:-35476855 | MS.gene059454:CDS |
| ATGGGTTCCTCGTACTCTTC+AGG | 0.321975 | 3.2:-35476836 | MS.gene059454:intron |
| TTGCTTACAGATCGAATATA+TGG | 0.343776 | 3.2:+35476669 | None:intergenic |
| GGATTCTTGATTCTTATATT+GGG | 0.348817 | 3.2:-35476891 | MS.gene059454:CDS |
| TCCACAATCATTCCCAAAAT+AGG | 0.361752 | 3.2:+35476864 | None:intergenic |
| CATGAAACGTAGTAATTTCA+AGG | 0.366317 | 3.2:-35477041 | MS.gene059454:intron |
| GTGTAGGGGACAGTCTCATT+TGG | 0.375348 | 3.2:-35476918 | MS.gene059454:intron |
| GAATGATTGTGGAATCTTAT+GGG | 0.399827 | 3.2:-35476854 | MS.gene059454:CDS |
| TGTAGGGGACAGTCTCATTT+GGG | 0.433564 | 3.2:-35476917 | MS.gene059454:intron |
| TGGATTTCAGTCCAATGGTC+AGG | 0.446122 | 3.2:+35477077 | None:intergenic |
| TCTTGATTCTTATATTGGGA+TGG | 0.451561 | 3.2:-35476887 | MS.gene059454:CDS |
| AGAGGATTCCTGTTCTTGGT+TGG | 0.473898 | 3.2:-35476704 | MS.gene059454:CDS |
| TGAACAAACCAACCAAGAAC+AGG | 0.496033 | 3.2:+35476696 | None:intergenic |
| TCTGGAGTATCCCTGACCAT+TGG | 0.509875 | 3.2:-35477088 | MS.gene059454:CDS |
| GGGACAGTCTCATTTGGGAT+AGG | 0.538151 | 3.2:-35476912 | MS.gene059454:CDS |
| CAGATCCAGAGCTGTTACAC+AGG | 0.547574 | 3.2:+35476249 | None:intergenic |
| CATGGTGGATTTCAGTCCAA+TGG | 0.562388 | 3.2:+35477072 | None:intergenic |
| TGAACGCTATCGAGGTAGGC+GGG | 0.562724 | 3.2:-35476274 | MS.gene059454:CDS |
| TGTTTGAACGCTATCGAGGT+AGG | 0.608217 | 3.2:-35476278 | MS.gene059454:CDS |
| GGATTTCAGTCCAATGGTCA+GGG | 0.609217 | 3.2:+35477078 | None:intergenic |
| ACGTTTCATGAAGAATTGCA+TGG | 0.620008 | 3.2:+35477054 | None:intergenic |
| TTGAACGCTATCGAGGTAGG+CGG | 0.621081 | 3.2:-35476275 | MS.gene059454:CDS |
| CAGCTGTTTGAACGCTATCG+AGG | 0.639687 | 3.2:-35476282 | MS.gene059454:CDS |
| GGGATACTCCAGAAACAAAG+AGG | 0.640829 | 3.2:+35477098 | None:intergenic |
| TGCAAGAAAACTGACAGTGT+AGG | 0.675829 | 3.2:+35476726 | None:intergenic |
| AACAAAACCTGAAGAGTACG+AGG | 0.678654 | 3.2:+35476829 | None:intergenic |
| TTTCATGAAGAATTGCATGG+TGG | 0.687707 | 3.2:+35477057 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATAAATATTTGAATGAA+AGG | + | chr3.2:35477276-35477295 | None:intergenic | 10.0% |
| !! | TTTCATTCAAATATTTATAT+AGG | - | chr3.2:35477275-35477294 | MS.gene059454:intron | 10.0% |
| !!! | ATTTTTATTTTTGTTTATGC+TGG | - | chr3.2:35476293-35476312 | MS.gene059454:intron | 15.0% |
| !! | ATGTAAATACAATTACAGAT+AGG | + | chr3.2:35476485-35476504 | None:intergenic | 20.0% |
| ! | AGGATTCTTGATTCTTATAT+TGG | - | chr3.2:35477006-35477025 | MS.gene059454:intron | 25.0% |
| ! | ATGAACATCCATTAATTATG+TGG | + | chr3.2:35477358-35477377 | None:intergenic | 25.0% |
| ! | GGATTCTTGATTCTTATATT+GGG | - | chr3.2:35477007-35477026 | MS.gene059454:intron | 25.0% |
| ! | TTGTAAATTCAATTCTGACA+TGG | - | chr3.2:35476420-35476439 | MS.gene059454:intron | 25.0% |
| ! | TTGTCATATCTAATTGCTAA+TGG | - | chr3.2:35477121-35477140 | MS.gene059454:intron | 25.0% |
| !! | ACATAATTAATGGATGTTCA+TGG | - | chr3.2:35477357-35477376 | MS.gene059454:intron | 25.0% |
| ACATAAAACAGAGATTGGAT+TGG | - | chr3.2:35476584-35476603 | MS.gene059454:intron | 30.0% | |
| ATGTCATGCAAATTAATGCA+TGG | + | chr3.2:35477559-35477578 | None:intergenic | 30.0% | |
| CATAAAACAGAGATTGGATT+GGG | - | chr3.2:35476585-35476604 | MS.gene059454:intron | 30.0% | |
| CATATCTAATTGCTAATGGT+GGG | - | chr3.2:35477125-35477144 | MS.gene059454:intron | 30.0% | |
| CATGAAACGTAGTAATTTCA+AGG | - | chr3.2:35476857-35476876 | MS.gene059454:CDS | 30.0% | |
| CTAATATACGTGTTTGCATT+TGG | - | chr3.2:35476560-35476579 | MS.gene059454:intron | 30.0% | |
| GAATGATTGTGGAATCTTAT+GGG | - | chr3.2:35477044-35477063 | MS.gene059454:CDS | 30.0% | |
| GTTATATTCTTCTTCGACAA+GGG | - | chr3.2:35476645-35476664 | MS.gene059454:intron | 30.0% | |
| TACATTTATACCAGTATGCA+TGG | - | chr3.2:35477330-35477349 | MS.gene059454:intron | 30.0% | |
| TATCATTATGTGGTTGTGTA+GGG | - | chr3.2:35476965-35476984 | MS.gene059454:intron | 30.0% | |
| TCATATCTAATTGCTAATGG+TGG | - | chr3.2:35477124-35477143 | MS.gene059454:intron | 30.0% | |
| TCTGTCAAGTGATTATTGAA+TGG | - | chr3.2:35477471-35477490 | MS.gene059454:intron | 30.0% | |
| TCTTGATTCTTATATTGGGA+TGG | - | chr3.2:35477011-35477030 | MS.gene059454:intron | 30.0% | |
| TGAACACAAAATGAAAGAGT+GGG | + | chr3.2:35476902-35476921 | None:intergenic | 30.0% | |
| TGATTTGAGAGATGAAACAA+AGG | + | chr3.2:35476777-35476796 | None:intergenic | 30.0% | |
| TTATCATTATGTGGTTGTGT+AGG | - | chr3.2:35476964-35476983 | MS.gene059454:intron | 30.0% | |
| TTGAACACAAAATGAAAGAG+TGG | + | chr3.2:35476903-35476922 | None:intergenic | 30.0% | |
| TTGCTTACAGATCGAATATA+TGG | + | chr3.2:35477232-35477251 | None:intergenic | 30.0% | |
| TTGGAACATAAAACAGAGAT+TGG | - | chr3.2:35476579-35476598 | MS.gene059454:intron | 30.0% | |
| ! | GGTATATTTTTCTCATTCCT+TGG | - | chr3.2:35476621-35476640 | MS.gene059454:intron | 30.0% |
| ACGTTTCATGAAGAATTGCA+TGG | + | chr3.2:35476847-35476866 | None:intergenic | 35.0% | |
| ATCATTATGTGGTTGTGTAG+GGG | - | chr3.2:35476966-35476985 | MS.gene059454:intron | 35.0% | |
| CACGTCAACTTATCATTATG+TGG | - | chr3.2:35476955-35476974 | MS.gene059454:intron | 35.0% | |
| CGTTATATTCTTCTTCGACA+AGG | - | chr3.2:35476644-35476663 | MS.gene059454:intron | 35.0% | |
| GCATGGTACCACATAATTAA+TGG | - | chr3.2:35477347-35477366 | MS.gene059454:intron | 35.0% | |
| GGAATGATTGTGGAATCTTA+TGG | - | chr3.2:35477043-35477062 | MS.gene059454:CDS | 35.0% | |
| TCAAGTGATTATTGAATGGC+AGG | - | chr3.2:35477475-35477494 | MS.gene059454:intron | 35.0% | |
| TCCACAATCATTCCCAAAAT+AGG | + | chr3.2:35477037-35477056 | None:intergenic | 35.0% | |
| TTACAGATAGGACAAGTAGA+TGG | + | chr3.2:35476473-35476492 | None:intergenic | 35.0% | |
| TTTCATGAAGAATTGCATGG+TGG | + | chr3.2:35476844-35476863 | None:intergenic | 35.0% | |
| ! | AATCAGATCCTCTTTGTTTC+TGG | - | chr3.2:35476792-35476811 | MS.gene059454:intron | 35.0% |
| ! | ATATTGGGATGGCCTATTTT+GGG | - | chr3.2:35477022-35477041 | MS.gene059454:intron | 35.0% |
| ! | CACATAATGATAAGTTGACG+TGG | + | chr3.2:35476957-35476976 | None:intergenic | 35.0% |
| ! | TATATTGGGATGGCCTATTT+TGG | - | chr3.2:35477021-35477040 | MS.gene059454:intron | 35.0% |
| ! | TTATATTTTGCAGTGGCTTC+TGG | - | chr3.2:35477149-35477168 | MS.gene059454:intron | 35.0% |
| ! | TTTTCTCTGATCTTCTTTCG+TGG | - | chr3.2:35476923-35476942 | MS.gene059454:intron | 35.0% |
| CGAAGAAGAATATAACGCCA+AGG | + | chr3.2:35476641-35476660 | None:intergenic | 40.0% | |
| TGAACAAACCAACCAAGAAC+AGG | + | chr3.2:35477205-35477224 | None:intergenic | 40.0% | |
| TGCAAGAAAACTGACAGTGT+AGG | + | chr3.2:35477175-35477194 | None:intergenic | 40.0% | |
| TTATGTGGTACCATGCATAC+TGG | + | chr3.2:35477343-35477362 | None:intergenic | 40.0% | |
| ! | AACAAAACCTGAAGAGTACG+AGG | + | chr3.2:35477072-35477091 | None:intergenic | 40.0% |
| ! | GAGATTGGATTGGGATTAAC+AGG | - | chr3.2:35476594-35476613 | MS.gene059454:intron | 40.0% |
| ! | GGTGGGATTATATTTTGCAG+TGG | - | chr3.2:35477142-35477161 | MS.gene059454:intron | 40.0% |
| !! | GCCTATTTTGGGAATGATTG+TGG | - | chr3.2:35477033-35477052 | MS.gene059454:intron | 40.0% |
| AGAGGATTCCTGTTCTTGGT+TGG | - | chr3.2:35477194-35477213 | MS.gene059454:intron | 45.0% | |
| ATTGCAGCTGTGACAGATTG+TGG | - | chr3.2:35477392-35477411 | MS.gene059454:intron | 45.0% | |
| GGATTGGGATTAACAGGCTT+TGG | - | chr3.2:35476600-35476619 | MS.gene059454:intron | 45.0% | |
| GGATTTCAGTCCAATGGTCA+GGG | + | chr3.2:35476823-35476842 | None:intergenic | 45.0% | |
| GGGATACTCCAGAAACAAAG+AGG | + | chr3.2:35476803-35476822 | None:intergenic | 45.0% | |
| TGGATTTCAGTCCAATGGTC+AGG | + | chr3.2:35476824-35476843 | None:intergenic | 45.0% | |
| TGTAGGGGACAGTCTCATTT+GGG | - | chr3.2:35476981-35477000 | MS.gene059454:intron | 45.0% | |
| TGTTTGAACGCTATCGAGGT+AGG | - | chr3.2:35477620-35477639 | MS.gene059454:intron | 45.0% | |
| TTGCAGAGGATTCCTGTTCT+TGG | - | chr3.2:35477190-35477209 | MS.gene059454:intron | 45.0% | |
| TTGCAGCTGTGACAGATTGT+GGG | - | chr3.2:35477393-35477412 | MS.gene059454:intron | 45.0% | |
| ! | CACTGTCAGTTTTCTTGCAG+AGG | - | chr3.2:35477176-35477195 | MS.gene059454:intron | 45.0% |
| !! | CATGGTGGATTTCAGTCCAA+TGG | + | chr3.2:35476829-35476848 | None:intergenic | 45.0% |
| !! | GACAAGGGTTTGCTAGCAAT+GGG | - | chr3.2:35476660-35476679 | MS.gene059454:intron | 45.0% |
| ATGGGTTCCTCGTACTCTTC+AGG | - | chr3.2:35477062-35477081 | MS.gene059454:CDS | 50.0% | |
| CAGCTGCATGTCGACAGAAA+AGG | + | chr3.2:35477602-35477621 | None:intergenic | 50.0% | |
| CAGCTGTTTGAACGCTATCG+AGG | - | chr3.2:35477616-35477635 | MS.gene059454:intron | 50.0% | |
| GTGTAGGGGACAGTCTCATT+TGG | - | chr3.2:35476980-35476999 | MS.gene059454:intron | 50.0% | |
| TGCAGCTGTGACAGATTGTG+GGG | - | chr3.2:35477394-35477413 | MS.gene059454:intron | 50.0% | |
| TTGAACGCTATCGAGGTAGG+CGG | - | chr3.2:35477623-35477642 | MS.gene059454:intron | 50.0% | |
| ! | GGGACAGTCTCATTTGGGAT+AGG | - | chr3.2:35476986-35477005 | MS.gene059454:intron | 50.0% |
| ! | TCTGGAGTATCCCTGACCAT+TGG | - | chr3.2:35476810-35476829 | MS.gene059454:intron | 50.0% |
| !! | CGACAAGGGTTTGCTAGCAA+TGG | - | chr3.2:35476659-35476678 | MS.gene059454:intron | 50.0% |
| GCAGCTGTGACAGATTGTGG+GGG | - | chr3.2:35477395-35477414 | MS.gene059454:intron | 55.0% | |
| TGAACGCTATCGAGGTAGGC+GGG | - | chr3.2:35477624-35477643 | MS.gene059454:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 35476263 | 35477657 | 35476263 | ID=MS.gene059454 |
| chr3.2 | mRNA | 35476263 | 35477657 | 35476263 | ID=MS.gene059454.t1;Parent=MS.gene059454 |
| chr3.2 | exon | 35477646 | 35477657 | 35477646 | ID=MS.gene059454.t1.exon1;Parent=MS.gene059454.t1 |
| chr3.2 | CDS | 35477646 | 35477657 | 35477646 | ID=cds.MS.gene059454.t1;Parent=MS.gene059454.t1 |
| chr3.2 | exon | 35477042 | 35477122 | 35477042 | ID=MS.gene059454.t1.exon2;Parent=MS.gene059454.t1 |
| chr3.2 | CDS | 35477042 | 35477122 | 35477042 | ID=cds.MS.gene059454.t1;Parent=MS.gene059454.t1 |
| chr3.2 | exon | 35476837 | 35476934 | 35476837 | ID=MS.gene059454.t1.exon3;Parent=MS.gene059454.t1 |
| chr3.2 | CDS | 35476837 | 35476934 | 35476837 | ID=cds.MS.gene059454.t1;Parent=MS.gene059454.t1 |
| chr3.2 | exon | 35476677 | 35476758 | 35476677 | ID=MS.gene059454.t1.exon4;Parent=MS.gene059454.t1 |
| chr3.2 | CDS | 35476677 | 35476758 | 35476677 | ID=cds.MS.gene059454.t1;Parent=MS.gene059454.t1 |
| chr3.2 | exon | 35476263 | 35476301 | 35476263 | ID=MS.gene059454.t1.exon5;Parent=MS.gene059454.t1 |
| chr3.2 | CDS | 35476263 | 35476301 | 35476263 | ID=cds.MS.gene059454.t1;Parent=MS.gene059454.t1 |
| Gene Sequence |
| Protein sequence |
