Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28918.t1 | RHN61833.1 | 96.2 | 291 | 11 | 0 | 1 | 291 | 488 | 778 | 6.40E-153 | 550.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28918.t1 | G7JV08 | 96.2 | 291 | 11 | 0 | 1 | 291 | 474 | 764 | 4.6e-153 | 550.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene28918 | 0.837868 | 4.03E-57 | -1.69E-46 |
| MS.gene04918 | MS.gene28918 | 0.880178 | 6.80E-70 | -1.69E-46 |
| MS.gene04919 | MS.gene28918 | 0.830924 | 2.23E-55 | -1.69E-46 |
| MS.gene04920 | MS.gene28918 | 0.86037 | 2.17E-63 | -1.69E-46 |
| MS.gene049254 | MS.gene28918 | 0.817221 | 3.69E-52 | -1.69E-46 |
| MS.gene049283 | MS.gene28918 | 0.839734 | 1.33E-57 | -1.69E-46 |
| MS.gene049343 | MS.gene28918 | 0.812399 | 4.32E-51 | -1.69E-46 |
| MS.gene049350 | MS.gene28918 | 0.803069 | 4.16E-49 | -1.69E-46 |
| MS.gene049368 | MS.gene28918 | 0.836623 | 8.40E-57 | -1.69E-46 |
| MS.gene049411 | MS.gene28918 | 0.843109 | 1.71E-58 | -1.69E-46 |
| MS.gene049499 | MS.gene28918 | 0.844942 | 5.53E-59 | -1.69E-46 |
| MS.gene049680 | MS.gene28918 | 0.861168 | 1.24E-63 | -1.69E-46 |
| MS.gene049711 | MS.gene28918 | 0.801089 | 1.06E-48 | -1.69E-46 |
| MS.gene049807 | MS.gene28918 | 0.812155 | 4.89E-51 | -1.69E-46 |
| MS.gene049821 | MS.gene28918 | 0.802668 | 5.03E-49 | -1.69E-46 |
| MS.gene049825 | MS.gene28918 | 0.811212 | 7.84E-51 | -1.69E-46 |
| MS.gene049873 | MS.gene28918 | 0.805348 | 1.39E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene28918 | 0.84635 | 2.30E-59 | -1.69E-46 |
| MS.gene05029 | MS.gene28918 | 0.805322 | 1.41E-49 | -1.69E-46 |
| MS.gene050352 | MS.gene28918 | 0.809061 | 2.28E-50 | -1.69E-46 |
| MS.gene050646 | MS.gene28918 | 0.82743 | 1.57E-54 | -1.69E-46 |
| MS.gene050654 | MS.gene28918 | 0.802651 | 5.07E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene28918 | 0.851521 | 8.41E-61 | -1.69E-46 |
| MS.gene050752 | MS.gene28918 | 0.825938 | 3.57E-54 | -1.69E-46 |
| MS.gene050838 | MS.gene28918 | 0.82657 | 2.52E-54 | -1.69E-46 |
| MS.gene050945 | MS.gene28918 | 0.851157 | 1.07E-60 | -1.69E-46 |
| MS.gene050955 | MS.gene28918 | 0.831185 | 1.93E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene28918 | 0.877843 | 4.54E-69 | -1.69E-46 |
| MS.gene051019 | MS.gene28918 | 0.813778 | 2.15E-51 | -1.69E-46 |
| MS.gene051043 | MS.gene28918 | 0.802831 | 4.66E-49 | -1.69E-46 |
| MS.gene051234 | MS.gene28918 | 0.868937 | 4.50E-66 | -1.69E-46 |
| MS.gene051343 | MS.gene28918 | 0.852763 | 3.73E-61 | -1.69E-46 |
| MS.gene051363 | MS.gene28918 | 0.841608 | 4.29E-58 | -1.69E-46 |
| MS.gene051365 | MS.gene28918 | 0.839992 | 1.14E-57 | -1.69E-46 |
| MS.gene051637 | MS.gene28918 | 0.810726 | 9.99E-51 | -1.69E-46 |
| MS.gene051676 | MS.gene28918 | 0.803859 | 2.85E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene28918 | 0.842079 | 3.22E-58 | -1.69E-46 |
| MS.gene051744 | MS.gene28918 | 0.82253 | 2.26E-53 | -1.69E-46 |
| MS.gene05188 | MS.gene28918 | 0.828488 | 8.74E-55 | -1.69E-46 |
| MS.gene051902 | MS.gene28918 | 0.848429 | 6.16E-60 | -1.69E-46 |
| MS.gene051975 | MS.gene28918 | 0.859776 | 3.27E-63 | -1.69E-46 |
| MS.gene052072 | MS.gene28918 | 0.847472 | 1.13E-59 | -1.69E-46 |
| MS.gene052307 | MS.gene28918 | 0.81986 | 9.30E-53 | -1.69E-46 |
| MS.gene052335 | MS.gene28918 | 0.812427 | 4.26E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene28918 | 0.804616 | 1.98E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene28918 | 0.841281 | 5.23E-58 | -1.69E-46 |
| MS.gene052516 | MS.gene28918 | 0.827217 | 1.77E-54 | -1.69E-46 |
| MS.gene052518 | MS.gene28918 | 0.849092 | 4.04E-60 | -1.69E-46 |
| MS.gene052519 | MS.gene28918 | 0.842843 | 2.02E-58 | -1.69E-46 |
| MS.gene052533 | MS.gene28918 | 0.843307 | 1.52E-58 | -1.69E-46 |
| MS.gene052584 | MS.gene28918 | 0.810922 | 9.06E-51 | -1.69E-46 |
| MS.gene052685 | MS.gene28918 | 0.809258 | 2.07E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene28918 | 0.804247 | 2.37E-49 | -1.69E-46 |
| MS.gene052793 | MS.gene28918 | 0.825876 | 3.69E-54 | -1.69E-46 |
| MS.gene052889 | MS.gene28918 | 0.804234 | 2.38E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene28918 | 0.807476 | 4.96E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene28918 | 0.803958 | 2.72E-49 | -1.69E-46 |
| MS.gene053058 | MS.gene28918 | 0.814834 | 1.26E-51 | -1.69E-46 |
| MS.gene053079 | MS.gene28918 | 0.815461 | 9.14E-52 | -1.69E-46 |
| MS.gene053122 | MS.gene28918 | 0.828127 | 1.07E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene28918 | 0.84588 | 3.08E-59 | -1.69E-46 |
| MS.gene053272 | MS.gene28918 | -0.838941 | 2.13E-57 | -1.69E-46 |
| MS.gene053298 | MS.gene28918 | 0.803735 | 3.02E-49 | -1.69E-46 |
| MS.gene05364 | MS.gene28918 | 0.829696 | 4.46E-55 | -1.69E-46 |
| MS.gene053879 | MS.gene28918 | 0.811655 | 6.28E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene28918 | 0.832578 | 8.73E-56 | -1.69E-46 |
| MS.gene053963 | MS.gene28918 | 0.805308 | 1.42E-49 | -1.69E-46 |
| MS.gene054004 | MS.gene28918 | 0.839209 | 1.82E-57 | -1.69E-46 |
| MS.gene054142 | MS.gene28918 | 0.847767 | 9.39E-60 | -1.69E-46 |
| MS.gene054178 | MS.gene28918 | 0.819336 | 1.23E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene28918 | 0.829335 | 5.45E-55 | -1.69E-46 |
| MS.gene054413 | MS.gene28918 | 0.807945 | 3.94E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene28918 | 0.837583 | 4.77E-57 | -1.69E-46 |
| MS.gene054651 | MS.gene28918 | 0.859305 | 4.53E-63 | -1.69E-46 |
| MS.gene05469 | MS.gene28918 | 0.809575 | 1.77E-50 | -1.69E-46 |
| MS.gene054709 | MS.gene28918 | 0.819604 | 1.06E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene28918 | 0.802258 | 6.11E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene28918 | 0.808469 | 3.05E-50 | -1.69E-46 |
| MS.gene054957 | MS.gene28918 | 0.82269 | 2.07E-53 | -1.69E-46 |
| MS.gene055150 | MS.gene28918 | 0.809338 | 1.99E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene28918 | 0.819818 | 9.51E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene28918 | 0.862341 | 5.43E-64 | -1.69E-46 |
| MS.gene055555 | MS.gene28918 | 0.828472 | 8.82E-55 | -1.69E-46 |
| MS.gene055765 | MS.gene28918 | 0.8089 | 2.47E-50 | -1.69E-46 |
| MS.gene055801 | MS.gene28918 | 0.806017 | 1.01E-49 | -1.69E-46 |
| MS.gene055841 | MS.gene28918 | 0.828845 | 7.17E-55 | -1.69E-46 |
| MS.gene056010 | MS.gene28918 | 0.808571 | 2.90E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene28918 | 0.819565 | 1.09E-52 | -1.69E-46 |
| MS.gene056215 | MS.gene28918 | 0.844533 | 7.13E-59 | -1.69E-46 |
| MS.gene056308 | MS.gene28918 | 0.800294 | 1.54E-48 | -1.69E-46 |
| MS.gene056595 | MS.gene28918 | 0.803113 | 4.07E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene28918 | 0.849816 | 2.54E-60 | -1.69E-46 |
| MS.gene056776 | MS.gene28918 | 0.828973 | 6.67E-55 | -1.69E-46 |
| MS.gene056825 | MS.gene28918 | 0.800124 | 1.67E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene28918 | 0.836944 | 6.95E-57 | -1.69E-46 |
| MS.gene05689 | MS.gene28918 | 0.81353 | 2.44E-51 | -1.69E-46 |
| MS.gene05690 | MS.gene28918 | 0.824729 | 6.89E-54 | -1.69E-46 |
| MS.gene056986 | MS.gene28918 | 0.8583 | 9.06E-63 | -1.69E-46 |
| MS.gene057034 | MS.gene28918 | 0.823948 | 1.05E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene28918 | 0.907178 | 6.71E-81 | -1.69E-46 |
| MS.gene057208 | MS.gene28918 | 0.86331 | 2.73E-64 | -1.69E-46 |
| MS.gene057684 | MS.gene28918 | -0.804532 | 2.06E-49 | -1.69E-46 |
| MS.gene057694 | MS.gene28918 | 0.818591 | 1.81E-52 | -1.69E-46 |
| MS.gene057705 | MS.gene28918 | 0.839616 | 1.42E-57 | -1.69E-46 |
| MS.gene05781 | MS.gene28918 | 0.814288 | 1.66E-51 | -1.69E-46 |
| MS.gene057890 | MS.gene28918 | -0.812216 | 4.74E-51 | -1.69E-46 |
| MS.gene057893 | MS.gene28918 | -0.807468 | 4.98E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene28918 | 0.86353 | 2.33E-64 | -1.69E-46 |
| MS.gene058009 | MS.gene28918 | 0.84802 | 8.00E-60 | -1.69E-46 |
| MS.gene058095 | MS.gene28918 | 0.813016 | 3.17E-51 | -1.69E-46 |
| MS.gene058149 | MS.gene28918 | 0.825719 | 4.02E-54 | -1.69E-46 |
| MS.gene058159 | MS.gene28918 | 0.825928 | 3.59E-54 | -1.69E-46 |
| MS.gene058279 | MS.gene28918 | 0.823293 | 1.50E-53 | -1.69E-46 |
| MS.gene058781 | MS.gene28918 | 0.865053 | 7.80E-65 | -1.69E-46 |
| MS.gene058783 | MS.gene28918 | 0.877568 | 5.66E-69 | -1.69E-46 |
| MS.gene059146 | MS.gene28918 | 0.880942 | 3.62E-70 | -1.69E-46 |
| MS.gene059170 | MS.gene28918 | 0.810343 | 1.21E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene28918 | 0.818705 | 1.71E-52 | -1.69E-46 |
| MS.gene059454 | MS.gene28918 | 0.805524 | 1.28E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene28918 | 0.832947 | 7.07E-56 | -1.69E-46 |
| MS.gene059625 | MS.gene28918 | 0.814235 | 1.71E-51 | -1.69E-46 |
| MS.gene059778 | MS.gene28918 | 0.838894 | 2.19E-57 | -1.69E-46 |
| MS.gene059842 | MS.gene28918 | 0.805458 | 1.32E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene28918 | -0.895437 | 9.59E-76 | -1.69E-46 |
| MS.gene059901 | MS.gene28918 | -0.855222 | 7.29E-62 | -1.69E-46 |
| MS.gene059935 | MS.gene28918 | 0.816139 | 6.45E-52 | -1.69E-46 |
| MS.gene059965 | MS.gene28918 | 0.809481 | 1.85E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene28918 | 0.819012 | 1.45E-52 | -1.69E-46 |
| MS.gene060244 | MS.gene28918 | 0.836169 | 1.10E-56 | -1.69E-46 |
| MS.gene060259 | MS.gene28918 | 0.803221 | 3.87E-49 | -1.69E-46 |
| MS.gene060338 | MS.gene28918 | 0.842973 | 1.86E-58 | -1.69E-46 |
| MS.gene060365 | MS.gene28918 | 0.837226 | 5.89E-57 | -1.69E-46 |
| MS.gene060429 | MS.gene28918 | 0.810096 | 1.37E-50 | -1.69E-46 |
| MS.gene060435 | MS.gene28918 | 0.801511 | 8.70E-49 | -1.69E-46 |
| MS.gene060512 | MS.gene28918 | 0.808156 | 3.56E-50 | -1.69E-46 |
| MS.gene060527 | MS.gene28918 | 0.8024 | 5.71E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene28918 | 0.835675 | 1.46E-56 | -1.69E-46 |
| MS.gene060933 | MS.gene28918 | 0.822141 | 2.78E-53 | -1.69E-46 |
| MS.gene060935 | MS.gene28918 | 0.890646 | 8.16E-74 | -1.69E-46 |
| MS.gene060937 | MS.gene28918 | 0.890592 | 8.57E-74 | -1.69E-46 |
| MS.gene061100 | MS.gene28918 | 0.878251 | 3.27E-69 | -1.69E-46 |
| MS.gene061160 | MS.gene28918 | 0.845683 | 3.49E-59 | -1.69E-46 |
| MS.gene061188 | MS.gene28918 | 0.829241 | 5.75E-55 | -1.69E-46 |
| MS.gene061243 | MS.gene28918 | 0.816217 | 6.20E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28918.t1 | MTR_4g079510 | 96.220 | 291 | 11 | 0 | 1 | 291 | 474 | 764 | 0.0 | 582 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28918.t1 | AT3G58110 | 41.751 | 297 | 156 | 9 | 2 | 291 | 465 | 751 | 5.81e-61 | 206 |
| MS.gene28918.t1 | AT3G58110 | 41.751 | 297 | 156 | 9 | 2 | 291 | 495 | 781 | 6.73e-61 | 206 |
| MS.gene28918.t1 | AT2G42370 | 40.977 | 266 | 149 | 5 | 6 | 270 | 443 | 701 | 7.37e-58 | 197 |
Find 69 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGCTTGTTGCTGCCATTT+AGG | 0.183848 | 4.2:-30726321 | None:intergenic |
| CAATATCTTTACAGTTCTTT+TGG | 0.200628 | 4.2:-30726819 | None:intergenic |
| GCAGCTTCTGCTTCACTTAC+TGG | 0.206646 | 4.2:-30727003 | None:intergenic |
| ACGCTTGTTGCTGCCATTTA+GGG | 0.245951 | 4.2:-30726320 | None:intergenic |
| TACAAGGATGTTCCTGGATC+TGG | 0.260398 | 4.2:+30726703 | MS.gene28918:CDS |
| AACTCTCACCATACCCTAAA+TGG | 0.273884 | 4.2:+30726307 | MS.gene28918:CDS |
| CAATCTTTGCTTTATGCAAA+TGG | 0.314284 | 4.2:-30726525 | None:intergenic |
| ATCCTTGGGTGACAAGTTGC+AGG | 0.333008 | 4.2:+30726897 | MS.gene28918:CDS |
| GAAGCAGTGCAGATGCCTTT+TGG | 0.347443 | 4.2:+30726157 | MS.gene28918:CDS |
| ACAAGGATGTTCCTGGATCT+GGG | 0.360505 | 4.2:+30726704 | MS.gene28918:CDS |
| CCACTTTACAAGGATGTTCC+TGG | 0.370467 | 4.2:+30726697 | MS.gene28918:CDS |
| CGACAAAGTAAAACATTTGA+AGG | 0.384383 | 4.2:+30726927 | MS.gene28918:CDS |
| GGAACAGATGCAACATTTCA+TGG | 0.394956 | 4.2:+30726396 | MS.gene28918:CDS |
| GTGTTTCTTTCAAAGCATTT+CGG | 0.425904 | 4.2:-30726627 | None:intergenic |
| TTTCCTCTTCTTCCTTCCTC+AGG | 0.427992 | 4.2:-30726765 | None:intergenic |
| AATCTTTGCTTTATGCAAAT+GGG | 0.444722 | 4.2:-30726524 | None:intergenic |
| AAACACAGATGGAGGTATAT+CGG | 0.445628 | 4.2:+30726560 | MS.gene28918:CDS |
| GATGATCCTCAGATGATTCA+TGG | 0.446049 | 4.2:+30726232 | MS.gene28918:CDS |
| GATGTATGCATCAAAAGATC+AGG | 0.477067 | 4.2:+30726432 | MS.gene28918:CDS |
| ATGCATCCTTCGAAGTCCAT+TGG | 0.486850 | 4.2:-30726373 | None:intergenic |
| GAGCACATTCAACTGATTCA+AGG | 0.492428 | 4.2:-30726981 | None:intergenic |
| GCTACAGAGGAGAGATGAAA+TGG | 0.498927 | 4.2:+30726498 | MS.gene28918:CDS |
| TAGGAGATTTCCTCTCTGCT+AGG | 0.500380 | 4.2:+30726209 | MS.gene28918:CDS |
| GAGTGTGATGGATCTGGAAA+AGG | 0.500881 | 4.2:+30726738 | MS.gene28918:CDS |
| AAAGAACTGTAAAGATATTG+AGG | 0.501243 | 4.2:+30726822 | MS.gene28918:CDS |
| AGCTGATGAGCCACTTTACA+AGG | 0.501688 | 4.2:+30726687 | MS.gene28918:CDS |
| TTGAAGGAAGTGAATGCAAA+AGG | 0.505591 | 4.2:+30726943 | MS.gene28918:CDS |
| TCTAAGCAGAGTTGAATCCT+TGG | 0.512561 | 4.2:+30726882 | MS.gene28918:CDS |
| CAAGGATGTTCCTGGATCTG+GGG | 0.516329 | 4.2:+30726705 | MS.gene28918:CDS |
| TGAGGAAGAGTGGCTTGGCC+AGG | 0.516986 | 4.2:+30726840 | MS.gene28918:CDS |
| CTAAGCAGAGTTGAATCCTT+GGG | 0.518875 | 4.2:+30726883 | MS.gene28918:CDS |
| AACATTTCATGGAGAAAGCT+AGG | 0.521784 | 4.2:+30726407 | MS.gene28918:CDS |
| TGTTGCTGCCATTTAGGGTA+TGG | 0.530516 | 4.2:-30726315 | None:intergenic |
| CATCTTCATGATAGCTCGGT+AGG | 0.547568 | 4.2:+30726190 | MS.gene28918:CDS |
| AAGGATGTTCCTGGATCTGG+GGG | 0.550583 | 4.2:+30726706 | MS.gene28918:CDS |
| CCAGGAACATCCTTGTAAAG+TGG | 0.554028 | 4.2:-30726697 | None:intergenic |
| AGCACATTCAACTGATTCAA+GGG | 0.558315 | 4.2:-30726980 | None:intergenic |
| AGATGAATCCCAGAACCAAA+AGG | 0.567276 | 4.2:-30726172 | None:intergenic |
| TCACAAGAGAGACATTGGTC+TGG | 0.567667 | 4.2:+30726276 | MS.gene28918:CDS |
| TTATATGATGCAAAGCCTTG+TGG | 0.568831 | 4.2:+30726597 | MS.gene28918:CDS |
| AGGAGATTTCCTCTCTGCTA+GGG | 0.569856 | 4.2:+30726210 | MS.gene28918:CDS |
| CCCATGGAGTTCTCAACCAA+TGG | 0.572312 | 4.2:+30726357 | MS.gene28918:CDS |
| GGAAAAGGAACGCCTGAGGA+AGG | 0.573524 | 4.2:+30726753 | MS.gene28918:CDS |
| AATGGACTTCGAAGGATGCA+TGG | 0.578842 | 4.2:+30726375 | MS.gene28918:CDS |
| TCCATTGGTTGAGAACTCCA+TGG | 0.588271 | 4.2:-30726358 | None:intergenic |
| GTAAAGATATTGAGGAAGAG+TGG | 0.590006 | 4.2:+30726830 | MS.gene28918:CDS |
| ACGCCTGAGGAAGGAAGAAG+AGG | 0.591628 | 4.2:+30726762 | MS.gene28918:CDS |
| CCAGACTCATAAAACACAGA+TGG | 0.591662 | 4.2:+30726549 | MS.gene28918:CDS |
| GGAAGAGTGGCTTGGCCAGG+TGG | 0.592271 | 4.2:+30726843 | MS.gene28918:CDS |
| GATTCATCTTCATGATAGCT+CGG | 0.592328 | 4.2:+30726186 | MS.gene28918:CDS |
| GATATTGAGGAAGAGTGGCT+TGG | 0.593907 | 4.2:+30726835 | MS.gene28918:CDS |
| AATGGTCACAAGAGAGACAT+TGG | 0.603182 | 4.2:+30726271 | MS.gene28918:CDS |
| CCATTGGTTGAGAACTCCAT+GGG | 0.610206 | 4.2:-30726357 | None:intergenic |
| GGAGGTATATCGGCTTGAGA+AGG | 0.615618 | 4.2:+30726570 | MS.gene28918:CDS |
| AGCCTGCAACTTGTCACCCA+AGG | 0.621091 | 4.2:-30726899 | None:intergenic |
| TAAAGTGGCTCATCAGCTTG+CGG | 0.624765 | 4.2:-30726682 | None:intergenic |
| GACTCATAAAACACAGATGG+AGG | 0.627538 | 4.2:+30726552 | MS.gene28918:CDS |
| GAAGAGTGGCTTGGCCAGGT+GGG | 0.628195 | 4.2:+30726844 | MS.gene28918:CDS |
| GATCCATCACACTCAAAACA+AGG | 0.636976 | 4.2:-30726729 | None:intergenic |
| TGAGGAAGGAAGAAGAGGAA+AGG | 0.640038 | 4.2:+30726767 | MS.gene28918:CDS |
| TCTCAACCAATGGACTTCGA+AGG | 0.640505 | 4.2:+30726367 | MS.gene28918:CDS |
| ATGATGCAAAGCCTTGTGGA+AGG | 0.642131 | 4.2:+30726601 | MS.gene28918:CDS |
| CATTTCGGTAGCCTTCCACA+AGG | 0.644036 | 4.2:-30726612 | None:intergenic |
| GACGAGCCATGAATCATCTG+AGG | 0.654353 | 4.2:-30726238 | None:intergenic |
| GCACATTCAACTGATTCAAG+GGG | 0.659949 | 4.2:-30726979 | None:intergenic |
| GTTTGAGAAGTGATAGCCCA+TGG | 0.662824 | 4.2:+30726341 | MS.gene28918:CDS |
| GTTCATTCAGTATACCCACC+TGG | 0.671321 | 4.2:-30726858 | None:intergenic |
| ATCTGGAAAAGGAACGCCTG+AGG | 0.684579 | 4.2:+30726749 | MS.gene28918:CDS |
| GAGGATCATCCCTAGCAGAG+AGG | 0.708652 | 4.2:-30726219 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAACTGTAAAGATATTG+AGG | + | chr4.2:30726822-30726841 | MS.gene28918:CDS | 25.0% |
| ! | AATCTTTGCTTTATGCAAAT+GGG | - | chr4.2:30726527-30726546 | None:intergenic | 25.0% |
| !! | CAATATCTTTACAGTTCTTT+TGG | - | chr4.2:30726822-30726841 | None:intergenic | 25.0% |
| CAATCTTTGCTTTATGCAAA+TGG | - | chr4.2:30726528-30726547 | None:intergenic | 30.0% | |
| CGACAAAGTAAAACATTTGA+AGG | + | chr4.2:30726927-30726946 | MS.gene28918:CDS | 30.0% | |
| ! | GTGTTTCTTTCAAAGCATTT+CGG | - | chr4.2:30726630-30726649 | None:intergenic | 30.0% |
| AACATTTCATGGAGAAAGCT+AGG | + | chr4.2:30726407-30726426 | MS.gene28918:CDS | 35.0% | |
| GATGTATGCATCAAAAGATC+AGG | + | chr4.2:30726432-30726451 | MS.gene28918:CDS | 35.0% | |
| GATTCATCTTCATGATAGCT+CGG | + | chr4.2:30726186-30726205 | MS.gene28918:CDS | 35.0% | |
| GTAAAGATATTGAGGAAGAG+TGG | + | chr4.2:30726830-30726849 | MS.gene28918:CDS | 35.0% | |
| TTATATGATGCAAAGCCTTG+TGG | + | chr4.2:30726597-30726616 | MS.gene28918:CDS | 35.0% | |
| TTGAAGGAAGTGAATGCAAA+AGG | + | chr4.2:30726943-30726962 | MS.gene28918:CDS | 35.0% | |
| ! | AAACACAGATGGAGGTATAT+CGG | + | chr4.2:30726560-30726579 | MS.gene28918:CDS | 35.0% |
| ! | AGCACATTCAACTGATTCAA+GGG | - | chr4.2:30726983-30727002 | None:intergenic | 35.0% |
| ! | TTTTGCTTAATGAGCTACAG+AGG | + | chr4.2:30726485-30726504 | MS.gene28918:CDS | 35.0% |
| AACTCTCACCATACCCTAAA+TGG | + | chr4.2:30726307-30726326 | MS.gene28918:CDS | 40.0% | |
| AATGGTCACAAGAGAGACAT+TGG | + | chr4.2:30726271-30726290 | MS.gene28918:CDS | 40.0% | |
| AGATGAATCCCAGAACCAAA+AGG | - | chr4.2:30726175-30726194 | None:intergenic | 40.0% | |
| CCAGACTCATAAAACACAGA+TGG | + | chr4.2:30726549-30726568 | MS.gene28918:CDS | 40.0% | |
| CTAAGCAGAGTTGAATCCTT+GGG | + | chr4.2:30726883-30726902 | MS.gene28918:CDS | 40.0% | |
| GACTCATAAAACACAGATGG+AGG | + | chr4.2:30726552-30726571 | MS.gene28918:CDS | 40.0% | |
| GATCCATCACACTCAAAACA+AGG | - | chr4.2:30726732-30726751 | None:intergenic | 40.0% | |
| GATGATCCTCAGATGATTCA+TGG | + | chr4.2:30726232-30726251 | MS.gene28918:CDS | 40.0% | |
| GGAACAGATGCAACATTTCA+TGG | + | chr4.2:30726396-30726415 | MS.gene28918:CDS | 40.0% | |
| TCTAAGCAGAGTTGAATCCT+TGG | + | chr4.2:30726882-30726901 | MS.gene28918:CDS | 40.0% | |
| ! | CCATCTGTGTTTTATGAGTC+TGG | - | chr4.2:30726552-30726571 | None:intergenic | 40.0% |
| ! | GAGCACATTCAACTGATTCA+AGG | - | chr4.2:30726984-30727003 | None:intergenic | 40.0% |
| ! | GCACATTCAACTGATTCAAG+GGG | - | chr4.2:30726982-30727001 | None:intergenic | 40.0% |
| !!! | ATTCATGGCTCGTCACTTTT+TGG | + | chr4.2:30726247-30726266 | MS.gene28918:CDS | 40.0% |
| !!! | TGTTTTGAGTGTGATGGATC+TGG | + | chr4.2:30726732-30726751 | MS.gene28918:CDS | 40.0% |
| ACAAGGATGTTCCTGGATCT+GGG | + | chr4.2:30726704-30726723 | MS.gene28918:CDS | 45.0% | |
| AGGAGATTTCCTCTCTGCTA+GGG | + | chr4.2:30726210-30726229 | MS.gene28918:CDS | 45.0% | |
| ATGCATCCTTCGAAGTCCAT+TGG | - | chr4.2:30726376-30726395 | None:intergenic | 45.0% | |
| CATCTTCATGATAGCTCGGT+AGG | + | chr4.2:30726190-30726209 | MS.gene28918:CDS | 45.0% | |
| CCACTTTACAAGGATGTTCC+TGG | + | chr4.2:30726697-30726716 | MS.gene28918:CDS | 45.0% | |
| CCAGGAACATCCTTGTAAAG+TGG | - | chr4.2:30726700-30726719 | None:intergenic | 45.0% | |
| GATATTGAGGAAGAGTGGCT+TGG | + | chr4.2:30726835-30726854 | MS.gene28918:CDS | 45.0% | |
| GCTACAGAGGAGAGATGAAA+TGG | + | chr4.2:30726498-30726517 | MS.gene28918:CDS | 45.0% | |
| GTTCATTCAGTATACCCACC+TGG | - | chr4.2:30726861-30726880 | None:intergenic | 45.0% | |
| GTTTGAGAAGTGATAGCCCA+TGG | + | chr4.2:30726341-30726360 | MS.gene28918:CDS | 45.0% | |
| TAAAGTGGCTCATCAGCTTG+CGG | - | chr4.2:30726685-30726704 | None:intergenic | 45.0% | |
| TACAAGGATGTTCCTGGATC+TGG | + | chr4.2:30726703-30726722 | MS.gene28918:CDS | 45.0% | |
| TAGGAGATTTCCTCTCTGCT+AGG | + | chr4.2:30726209-30726228 | MS.gene28918:CDS | 45.0% | |
| TCACAAGAGAGACATTGGTC+TGG | + | chr4.2:30726276-30726295 | MS.gene28918:CDS | 45.0% | |
| TGAGGAAGGAAGAAGAGGAA+AGG | + | chr4.2:30726767-30726786 | MS.gene28918:CDS | 45.0% | |
| TTTCCTCTTCTTCCTTCCTC+AGG | - | chr4.2:30726768-30726787 | None:intergenic | 45.0% | |
| ! | AACGCTTGTTGCTGCCATTT+AGG | - | chr4.2:30726324-30726343 | None:intergenic | 45.0% |
| ! | AATGGACTTCGAAGGATGCA+TGG | + | chr4.2:30726375-30726394 | MS.gene28918:CDS | 45.0% |
| ! | ACGCTTGTTGCTGCCATTTA+GGG | - | chr4.2:30726323-30726342 | None:intergenic | 45.0% |
| ! | AGCTGATGAGCCACTTTACA+AGG | + | chr4.2:30726687-30726706 | MS.gene28918:CDS | 45.0% |
| ! | ATGATGCAAAGCCTTGTGGA+AGG | + | chr4.2:30726601-30726620 | MS.gene28918:CDS | 45.0% |
| ! | CCATTGGTTGAGAACTCCAT+GGG | - | chr4.2:30726360-30726379 | None:intergenic | 45.0% |
| ! | TCCATTGGTTGAGAACTCCA+TGG | - | chr4.2:30726361-30726380 | None:intergenic | 45.0% |
| ! | TCTCAACCAATGGACTTCGA+AGG | + | chr4.2:30726367-30726386 | MS.gene28918:CDS | 45.0% |
| ! | TGTTGCTGCCATTTAGGGTA+TGG | - | chr4.2:30726318-30726337 | None:intergenic | 45.0% |
| !! | GAGTGTGATGGATCTGGAAA+AGG | + | chr4.2:30726738-30726757 | MS.gene28918:CDS | 45.0% |
| !!! | GGCTCGTCACTTTTTGGTAA+TGG | + | chr4.2:30726253-30726272 | MS.gene28918:CDS | 45.0% |
| !!! | TGCAGATGCCTTTTGGTTCT+GGG | + | chr4.2:30726164-30726183 | MS.gene28918:CDS | 45.0% |
| AAGGATGTTCCTGGATCTGG+GGG | + | chr4.2:30726706-30726725 | MS.gene28918:CDS | 50.0% | |
| ATCCTTGGGTGACAAGTTGC+AGG | + | chr4.2:30726897-30726916 | MS.gene28918:CDS | 50.0% | |
| ATCTGGAAAAGGAACGCCTG+AGG | + | chr4.2:30726749-30726768 | MS.gene28918:CDS | 50.0% | |
| CAAGGATGTTCCTGGATCTG+GGG | + | chr4.2:30726705-30726724 | MS.gene28918:CDS | 50.0% | |
| CATTTCGGTAGCCTTCCACA+AGG | - | chr4.2:30726615-30726634 | None:intergenic | 50.0% | |
| CCCATGGAGTTCTCAACCAA+TGG | + | chr4.2:30726357-30726376 | MS.gene28918:CDS | 50.0% | |
| GACGAGCCATGAATCATCTG+AGG | - | chr4.2:30726241-30726260 | None:intergenic | 50.0% | |
| GCAGCTTCTGCTTCACTTAC+TGG | - | chr4.2:30727006-30727025 | None:intergenic | 50.0% | |
| ! | GAAGCAGTGCAGATGCCTTT+TGG | + | chr4.2:30726157-30726176 | MS.gene28918:CDS | 50.0% |
| !! | GGAGGTATATCGGCTTGAGA+AGG | + | chr4.2:30726570-30726589 | MS.gene28918:CDS | 50.0% |
| !!! | GGGCCTTGTTTTGAGTGTGA+TGG | + | chr4.2:30726726-30726745 | MS.gene28918:CDS | 50.0% |
| !!! | GTGCAGATGCCTTTTGGTTC+TGG | + | chr4.2:30726163-30726182 | MS.gene28918:CDS | 50.0% |
| AAAACAAGGCCCCCAGATCC+AGG | - | chr4.2:30726718-30726737 | None:intergenic | 55.0% | |
| ACGCCTGAGGAAGGAAGAAG+AGG | + | chr4.2:30726762-30726781 | MS.gene28918:CDS | 55.0% | |
| AGCCTGCAACTTGTCACCCA+AGG | - | chr4.2:30726902-30726921 | None:intergenic | 55.0% | |
| GAGGATCATCCCTAGCAGAG+AGG | - | chr4.2:30726222-30726241 | None:intergenic | 55.0% | |
| GGAAAAGGAACGCCTGAGGA+AGG | + | chr4.2:30726753-30726772 | MS.gene28918:CDS | 55.0% | |
| ! | GAAGAGTGGCTTGGCCAGGT+GGG | + | chr4.2:30726844-30726863 | MS.gene28918:CDS | 60.0% |
| ! | TGAGGAAGAGTGGCTTGGCC+AGG | + | chr4.2:30726840-30726859 | MS.gene28918:CDS | 60.0% |
| ! | GGAAGAGTGGCTTGGCCAGG+TGG | + | chr4.2:30726843-30726862 | MS.gene28918:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 30726154 | 30727029 | 30726154 | ID=MS.gene28918 |
| chr4.2 | mRNA | 30726154 | 30727029 | 30726154 | ID=MS.gene28918.t1;Parent=MS.gene28918 |
| chr4.2 | exon | 30726154 | 30727029 | 30726154 | ID=MS.gene28918.t1.exon1;Parent=MS.gene28918.t1 |
| chr4.2 | CDS | 30726154 | 30727029 | 30726154 | ID=cds.MS.gene28918.t1;Parent=MS.gene28918.t1 |
| Gene Sequence |
| Protein sequence |
