AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73885


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73885.t1 MTR_4g079510 96.220 291 11 0 1 291 474 764 0.0 582
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73885.t1 AT3G58110 41.751 297 156 9 2 291 465 751 5.81e-61 206
MS.gene73885.t1 AT3G58110 41.751 297 156 9 2 291 495 781 6.73e-61 206
MS.gene73885.t1 AT2G42370 40.977 266 149 5 6 270 443 701 7.37e-58 197

Find 69 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGCTTGTTGCTGCCATTT+AGG 0.183848 4.2:-30674130 None:intergenic
CAATATCTTTACAGTTCTTT+TGG 0.200628 4.2:-30674628 None:intergenic
GCAGCTTCTGCTTCACTTAC+TGG 0.206646 4.2:-30674812 None:intergenic
ACGCTTGTTGCTGCCATTTA+GGG 0.245951 4.2:-30674129 None:intergenic
TACAAGGATGTTCCTGGATC+TGG 0.260398 4.2:+30674512 MS.gene73885:CDS
AACTCTCACCATACCCTAAA+TGG 0.273884 4.2:+30674116 MS.gene73885:CDS
CAATCTTTGCTTTATGCAAA+TGG 0.314284 4.2:-30674334 None:intergenic
ATCCTTGGGTGACAAGTTGC+AGG 0.333008 4.2:+30674706 MS.gene73885:CDS
GAAGCAGTGCAGATGCCTTT+TGG 0.347443 4.2:+30673966 MS.gene73885:CDS
ACAAGGATGTTCCTGGATCT+GGG 0.360505 4.2:+30674513 MS.gene73885:CDS
CCACTTTACAAGGATGTTCC+TGG 0.370467 4.2:+30674506 MS.gene73885:CDS
CGACAAAGTAAAACATTTGA+AGG 0.384383 4.2:+30674736 MS.gene73885:CDS
GGAACAGATGCAACATTTCA+TGG 0.394956 4.2:+30674205 MS.gene73885:CDS
GTGTTTCTTTCAAAGCATTT+CGG 0.425904 4.2:-30674436 None:intergenic
TTTCCTCTTCTTCCTTCCTC+AGG 0.427992 4.2:-30674574 None:intergenic
AATCTTTGCTTTATGCAAAT+GGG 0.444722 4.2:-30674333 None:intergenic
AAACACAGATGGAGGTATAT+CGG 0.445628 4.2:+30674369 MS.gene73885:CDS
GATGATCCTCAGATGATTCA+TGG 0.446049 4.2:+30674041 MS.gene73885:CDS
GATGTATGCATCAAAAGATC+AGG 0.477067 4.2:+30674241 MS.gene73885:CDS
ATGCATCCTTCGAAGTCCAT+TGG 0.486850 4.2:-30674182 None:intergenic
GAGCACATTCAACTGATTCA+AGG 0.492428 4.2:-30674790 None:intergenic
GCTACAGAGGAGAGATGAAA+TGG 0.498927 4.2:+30674307 MS.gene73885:CDS
TAGGAGATTTCCTCTCTGCT+AGG 0.500380 4.2:+30674018 MS.gene73885:CDS
GAGTGTGATGGATCTGGAAA+AGG 0.500881 4.2:+30674547 MS.gene73885:CDS
AAAGAACTGTAAAGATATTG+AGG 0.501243 4.2:+30674631 MS.gene73885:CDS
AGCTGATGAGCCACTTTACA+AGG 0.501688 4.2:+30674496 MS.gene73885:CDS
TTGAAGGAAGTGAATGCAAA+AGG 0.505591 4.2:+30674752 MS.gene73885:CDS
TCTAAGCAGAGTTGAATCCT+TGG 0.512561 4.2:+30674691 MS.gene73885:CDS
CAAGGATGTTCCTGGATCTG+GGG 0.516329 4.2:+30674514 MS.gene73885:CDS
TGAGGAAGAGTGGCTTGGCC+AGG 0.516986 4.2:+30674649 MS.gene73885:CDS
CTAAGCAGAGTTGAATCCTT+GGG 0.518875 4.2:+30674692 MS.gene73885:CDS
AACATTTCATGGAGAAAGCT+AGG 0.521784 4.2:+30674216 MS.gene73885:CDS
TGTTGCTGCCATTTAGGGTA+TGG 0.530516 4.2:-30674124 None:intergenic
CATCTTCATGATAGCTCGGT+AGG 0.547568 4.2:+30673999 MS.gene73885:CDS
AAGGATGTTCCTGGATCTGG+GGG 0.550583 4.2:+30674515 MS.gene73885:CDS
CCAGGAACATCCTTGTAAAG+TGG 0.554028 4.2:-30674506 None:intergenic
AGCACATTCAACTGATTCAA+GGG 0.558315 4.2:-30674789 None:intergenic
AGATGAATCCCAGAACCAAA+AGG 0.567276 4.2:-30673981 None:intergenic
TCACAAGAGAGACATTGGTC+TGG 0.567667 4.2:+30674085 MS.gene73885:CDS
TTATATGATGCAAAGCCTTG+TGG 0.568831 4.2:+30674406 MS.gene73885:CDS
AGGAGATTTCCTCTCTGCTA+GGG 0.569856 4.2:+30674019 MS.gene73885:CDS
CCCATGGAGTTCTCAACCAA+TGG 0.572312 4.2:+30674166 MS.gene73885:CDS
GGAAAAGGAACGCCTGAGGA+AGG 0.573524 4.2:+30674562 MS.gene73885:CDS
AATGGACTTCGAAGGATGCA+TGG 0.578842 4.2:+30674184 MS.gene73885:CDS
TCCATTGGTTGAGAACTCCA+TGG 0.588271 4.2:-30674167 None:intergenic
GTAAAGATATTGAGGAAGAG+TGG 0.590006 4.2:+30674639 MS.gene73885:CDS
ACGCCTGAGGAAGGAAGAAG+AGG 0.591628 4.2:+30674571 MS.gene73885:CDS
CCAGACTCATAAAACACAGA+TGG 0.591662 4.2:+30674358 MS.gene73885:CDS
GGAAGAGTGGCTTGGCCAGG+TGG 0.592271 4.2:+30674652 MS.gene73885:CDS
GATTCATCTTCATGATAGCT+CGG 0.592328 4.2:+30673995 MS.gene73885:CDS
GATATTGAGGAAGAGTGGCT+TGG 0.593907 4.2:+30674644 MS.gene73885:CDS
AATGGTCACAAGAGAGACAT+TGG 0.603182 4.2:+30674080 MS.gene73885:CDS
CCATTGGTTGAGAACTCCAT+GGG 0.610206 4.2:-30674166 None:intergenic
GGAGGTATATCGGCTTGAGA+AGG 0.615618 4.2:+30674379 MS.gene73885:CDS
AGCCTGCAACTTGTCACCCA+AGG 0.621091 4.2:-30674708 None:intergenic
TAAAGTGGCTCATCAGCTTG+CGG 0.624765 4.2:-30674491 None:intergenic
GACTCATAAAACACAGATGG+AGG 0.627538 4.2:+30674361 MS.gene73885:CDS
GAAGAGTGGCTTGGCCAGGT+GGG 0.628195 4.2:+30674653 MS.gene73885:CDS
GATCCATCACACTCAAAACA+AGG 0.636976 4.2:-30674538 None:intergenic
TGAGGAAGGAAGAAGAGGAA+AGG 0.640038 4.2:+30674576 MS.gene73885:CDS
TCTCAACCAATGGACTTCGA+AGG 0.640505 4.2:+30674176 MS.gene73885:CDS
ATGATGCAAAGCCTTGTGGA+AGG 0.642131 4.2:+30674410 MS.gene73885:CDS
CATTTCGGTAGCCTTCCACA+AGG 0.644036 4.2:-30674421 None:intergenic
GACGAGCCATGAATCATCTG+AGG 0.654353 4.2:-30674047 None:intergenic
GCACATTCAACTGATTCAAG+GGG 0.659949 4.2:-30674788 None:intergenic
GTTTGAGAAGTGATAGCCCA+TGG 0.662824 4.2:+30674150 MS.gene73885:CDS
GTTCATTCAGTATACCCACC+TGG 0.671321 4.2:-30674667 None:intergenic
ATCTGGAAAAGGAACGCCTG+AGG 0.684579 4.2:+30674558 MS.gene73885:CDS
GAGGATCATCCCTAGCAGAG+AGG 0.708652 4.2:-30674028 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAACTGTAAAGATATTG+AGG + chr4.2:30674631-30674650 MS.gene73885:CDS 25.0%
! AATCTTTGCTTTATGCAAAT+GGG - chr4.2:30674336-30674355 None:intergenic 25.0%
!! CAATATCTTTACAGTTCTTT+TGG - chr4.2:30674631-30674650 None:intergenic 25.0%
CAATCTTTGCTTTATGCAAA+TGG - chr4.2:30674337-30674356 None:intergenic 30.0%
CGACAAAGTAAAACATTTGA+AGG + chr4.2:30674736-30674755 MS.gene73885:CDS 30.0%
! GTGTTTCTTTCAAAGCATTT+CGG - chr4.2:30674439-30674458 None:intergenic 30.0%
AACATTTCATGGAGAAAGCT+AGG + chr4.2:30674216-30674235 MS.gene73885:CDS 35.0%
GATGTATGCATCAAAAGATC+AGG + chr4.2:30674241-30674260 MS.gene73885:CDS 35.0%
GATTCATCTTCATGATAGCT+CGG + chr4.2:30673995-30674014 MS.gene73885:CDS 35.0%
GTAAAGATATTGAGGAAGAG+TGG + chr4.2:30674639-30674658 MS.gene73885:CDS 35.0%
TTATATGATGCAAAGCCTTG+TGG + chr4.2:30674406-30674425 MS.gene73885:CDS 35.0%
TTGAAGGAAGTGAATGCAAA+AGG + chr4.2:30674752-30674771 MS.gene73885:CDS 35.0%
! AAACACAGATGGAGGTATAT+CGG + chr4.2:30674369-30674388 MS.gene73885:CDS 35.0%
! AGCACATTCAACTGATTCAA+GGG - chr4.2:30674792-30674811 None:intergenic 35.0%
! TTTTGCTTAATGAGCTACAG+AGG + chr4.2:30674294-30674313 MS.gene73885:CDS 35.0%
AACTCTCACCATACCCTAAA+TGG + chr4.2:30674116-30674135 MS.gene73885:CDS 40.0%
AATGGTCACAAGAGAGACAT+TGG + chr4.2:30674080-30674099 MS.gene73885:CDS 40.0%
AGATGAATCCCAGAACCAAA+AGG - chr4.2:30673984-30674003 None:intergenic 40.0%
CCAGACTCATAAAACACAGA+TGG + chr4.2:30674358-30674377 MS.gene73885:CDS 40.0%
CTAAGCAGAGTTGAATCCTT+GGG + chr4.2:30674692-30674711 MS.gene73885:CDS 40.0%
GACTCATAAAACACAGATGG+AGG + chr4.2:30674361-30674380 MS.gene73885:CDS 40.0%
GATCCATCACACTCAAAACA+AGG - chr4.2:30674541-30674560 None:intergenic 40.0%
GATGATCCTCAGATGATTCA+TGG + chr4.2:30674041-30674060 MS.gene73885:CDS 40.0%
GGAACAGATGCAACATTTCA+TGG + chr4.2:30674205-30674224 MS.gene73885:CDS 40.0%
TCTAAGCAGAGTTGAATCCT+TGG + chr4.2:30674691-30674710 MS.gene73885:CDS 40.0%
! CCATCTGTGTTTTATGAGTC+TGG - chr4.2:30674361-30674380 None:intergenic 40.0%
! GAGCACATTCAACTGATTCA+AGG - chr4.2:30674793-30674812 None:intergenic 40.0%
! GCACATTCAACTGATTCAAG+GGG - chr4.2:30674791-30674810 None:intergenic 40.0%
!!! ATTCATGGCTCGTCACTTTT+TGG + chr4.2:30674056-30674075 MS.gene73885:CDS 40.0%
!!! TGTTTTGAGTGTGATGGATC+TGG + chr4.2:30674541-30674560 MS.gene73885:CDS 40.0%
ACAAGGATGTTCCTGGATCT+GGG + chr4.2:30674513-30674532 MS.gene73885:CDS 45.0%
AGGAGATTTCCTCTCTGCTA+GGG + chr4.2:30674019-30674038 MS.gene73885:CDS 45.0%
ATGCATCCTTCGAAGTCCAT+TGG - chr4.2:30674185-30674204 None:intergenic 45.0%
CATCTTCATGATAGCTCGGT+AGG + chr4.2:30673999-30674018 MS.gene73885:CDS 45.0%
CCACTTTACAAGGATGTTCC+TGG + chr4.2:30674506-30674525 MS.gene73885:CDS 45.0%
CCAGGAACATCCTTGTAAAG+TGG - chr4.2:30674509-30674528 None:intergenic 45.0%
GATATTGAGGAAGAGTGGCT+TGG + chr4.2:30674644-30674663 MS.gene73885:CDS 45.0%
GCTACAGAGGAGAGATGAAA+TGG + chr4.2:30674307-30674326 MS.gene73885:CDS 45.0%
GTTCATTCAGTATACCCACC+TGG - chr4.2:30674670-30674689 None:intergenic 45.0%
GTTTGAGAAGTGATAGCCCA+TGG + chr4.2:30674150-30674169 MS.gene73885:CDS 45.0%
TAAAGTGGCTCATCAGCTTG+CGG - chr4.2:30674494-30674513 None:intergenic 45.0%
TACAAGGATGTTCCTGGATC+TGG + chr4.2:30674512-30674531 MS.gene73885:CDS 45.0%
TAGGAGATTTCCTCTCTGCT+AGG + chr4.2:30674018-30674037 MS.gene73885:CDS 45.0%
TCACAAGAGAGACATTGGTC+TGG + chr4.2:30674085-30674104 MS.gene73885:CDS 45.0%
TGAGGAAGGAAGAAGAGGAA+AGG + chr4.2:30674576-30674595 MS.gene73885:CDS 45.0%
TTTCCTCTTCTTCCTTCCTC+AGG - chr4.2:30674577-30674596 None:intergenic 45.0%
! AACGCTTGTTGCTGCCATTT+AGG - chr4.2:30674133-30674152 None:intergenic 45.0%
! AATGGACTTCGAAGGATGCA+TGG + chr4.2:30674184-30674203 MS.gene73885:CDS 45.0%
! ACGCTTGTTGCTGCCATTTA+GGG - chr4.2:30674132-30674151 None:intergenic 45.0%
! AGCTGATGAGCCACTTTACA+AGG + chr4.2:30674496-30674515 MS.gene73885:CDS 45.0%
! ATGATGCAAAGCCTTGTGGA+AGG + chr4.2:30674410-30674429 MS.gene73885:CDS 45.0%
! CCATTGGTTGAGAACTCCAT+GGG - chr4.2:30674169-30674188 None:intergenic 45.0%
! TCCATTGGTTGAGAACTCCA+TGG - chr4.2:30674170-30674189 None:intergenic 45.0%
! TCTCAACCAATGGACTTCGA+AGG + chr4.2:30674176-30674195 MS.gene73885:CDS 45.0%
! TGTTGCTGCCATTTAGGGTA+TGG - chr4.2:30674127-30674146 None:intergenic 45.0%
!! GAGTGTGATGGATCTGGAAA+AGG + chr4.2:30674547-30674566 MS.gene73885:CDS 45.0%
!!! GGCTCGTCACTTTTTGGTAA+TGG + chr4.2:30674062-30674081 MS.gene73885:CDS 45.0%
!!! TGCAGATGCCTTTTGGTTCT+GGG + chr4.2:30673973-30673992 MS.gene73885:CDS 45.0%
AAGGATGTTCCTGGATCTGG+GGG + chr4.2:30674515-30674534 MS.gene73885:CDS 50.0%
ATCCTTGGGTGACAAGTTGC+AGG + chr4.2:30674706-30674725 MS.gene73885:CDS 50.0%
ATCTGGAAAAGGAACGCCTG+AGG + chr4.2:30674558-30674577 MS.gene73885:CDS 50.0%
CAAGGATGTTCCTGGATCTG+GGG + chr4.2:30674514-30674533 MS.gene73885:CDS 50.0%
CATTTCGGTAGCCTTCCACA+AGG - chr4.2:30674424-30674443 None:intergenic 50.0%
CCCATGGAGTTCTCAACCAA+TGG + chr4.2:30674166-30674185 MS.gene73885:CDS 50.0%
GACGAGCCATGAATCATCTG+AGG - chr4.2:30674050-30674069 None:intergenic 50.0%
GCAGCTTCTGCTTCACTTAC+TGG - chr4.2:30674815-30674834 None:intergenic 50.0%
! GAAGCAGTGCAGATGCCTTT+TGG + chr4.2:30673966-30673985 MS.gene73885:CDS 50.0%
!! GGAGGTATATCGGCTTGAGA+AGG + chr4.2:30674379-30674398 MS.gene73885:CDS 50.0%
!!! GGGCCTTGTTTTGAGTGTGA+TGG + chr4.2:30674535-30674554 MS.gene73885:CDS 50.0%
!!! GTGCAGATGCCTTTTGGTTC+TGG + chr4.2:30673972-30673991 MS.gene73885:CDS 50.0%
AAAACAAGGCCCCCAGATCC+AGG - chr4.2:30674527-30674546 None:intergenic 55.0%
ACGCCTGAGGAAGGAAGAAG+AGG + chr4.2:30674571-30674590 MS.gene73885:CDS 55.0%
AGCCTGCAACTTGTCACCCA+AGG - chr4.2:30674711-30674730 None:intergenic 55.0%
GAGGATCATCCCTAGCAGAG+AGG - chr4.2:30674031-30674050 None:intergenic 55.0%
GGAAAAGGAACGCCTGAGGA+AGG + chr4.2:30674562-30674581 MS.gene73885:CDS 55.0%
! GAAGAGTGGCTTGGCCAGGT+GGG + chr4.2:30674653-30674672 MS.gene73885:CDS 60.0%
! TGAGGAAGAGTGGCTTGGCC+AGG + chr4.2:30674649-30674668 MS.gene73885:CDS 60.0%
! GGAAGAGTGGCTTGGCCAGG+TGG + chr4.2:30674652-30674671 MS.gene73885:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 30673963 30674838 30673963 ID=MS.gene73885
chr4.2 mRNA 30673963 30674838 30673963 ID=MS.gene73885.t1;Parent=MS.gene73885
chr4.2 exon 30673963 30674838 30673963 ID=MS.gene73885.t1.exon1;Parent=MS.gene73885.t1
chr4.2 CDS 30673963 30674838 30673963 ID=cds.MS.gene73885.t1;Parent=MS.gene73885.t1
Gene Sequence

>MS.gene73885

ATGGAAGCAGTGCAGATGCCTTTTGGTTCTGGGATTCATCTTCATGATAGCTCGGTAGGAGATTTCCTCTCTGCTAGGGATGATCCTCAGATGATTCATGGCTCGTCACTTTTTGGTAATGGTCACAAGAGAGACATTGGTCTGGATAATCATAACTCTCACCATACCCTAAATGGCAGCAACAAGCGTTTGAGAAGTGATAGCCCATGGAGTTCTCAACCAATGGACTTCGAAGGATGCATGGAACAGATGCAACATTTCATGGAGAAAGCTAGGATGATGTATGCATCAAAAGATCAGGCTGCTGAAGAATCTGCAGCAAATCAACAAGTTTTGCTTAATGAGCTACAGAGGAGAGATGAAATGGTTGCCCATTTGCATAAAGCAAAGATTGACCAGACTCATAAAACACAGATGGAGGTATATCGGCTTGAGAAGGAACTTTATATGATGCAAAGCCTTGTGGAAGGCTACCGAAATGCTTTGAAAGAAACACAAAAAGCTTTTGCTGACTATAGAGCACGTTGTCCGCAAGCTGATGAGCCACTTTACAAGGATGTTCCTGGATCTGGGGGCCTTGTTTTGAGTGTGATGGATCTGGAAAAGGAACGCCTGAGGAAGGAAGAAGAGGAAAGGATACAGTTGAGAGAACTTTTTAGAGATTTCCAAAAGAACTGTAAAGATATTGAGGAAGAGTGGCTTGGCCAGGTGGGTATACTGAATGAACATCTAAGCAGAGTTGAATCCTTGGGTGACAAGTTGCAGGCTCTTGACGACAAAGTAAAACATTTGAAGGAAGTGAATGCAAAAGGCAAGATTTCTGATCCCCTTGAATCAGTTGAATGTGCTCCAGTAAGTGAAGCAGAAGCTGCTTAG

Protein sequence

>MS.gene73885.t1

MEAVQMPFGSGIHLHDSSVGDFLSARDDPQMIHGSSLFGNGHKRDIGLDNHNSHHTLNGSNKRLRSDSPWSSQPMDFEGCMEQMQHFMEKARMMYASKDQAAEESAANQQVLLNELQRRDEMVAHLHKAKIDQTHKTQMEVYRLEKELYMMQSLVEGYRNALKETQKAFADYRARCPQADEPLYKDVPGSGGLVLSVMDLEKERLRKEEEERIQLRELFRDFQKNCKDIEEEWLGQVGILNEHLSRVESLGDKLQALDDKVKHLKEVNAKGKISDPLESVECAPVSEAEAA