AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73883


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73883.t1 MTR_4g079510 96.220 291 11 0 1 291 474 764 0.0 582
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73883.t1 AT3G58110 41.751 297 156 9 2 291 465 751 5.81e-61 206
MS.gene73883.t1 AT3G58110 41.751 297 156 9 2 291 495 781 6.73e-61 206
MS.gene73883.t1 AT2G42370 40.977 266 149 5 6 270 443 701 7.37e-58 197

Find 69 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGCTTGTTGCTGCCATTT+AGG 0.183848 4.2:-30632549 None:intergenic
CAATATCTTTACAGTTCTTT+TGG 0.200628 4.2:-30633047 None:intergenic
GCAGCTTCTGCTTCACTTAC+TGG 0.206646 4.2:-30633231 None:intergenic
ACGCTTGTTGCTGCCATTTA+GGG 0.245951 4.2:-30632548 None:intergenic
TACAAGGATGTTCCTGGATC+TGG 0.260398 4.2:+30632931 MS.gene73883:CDS
AACTCTCACCATACCCTAAA+TGG 0.273884 4.2:+30632535 MS.gene73883:CDS
CAATCTTTGCTTTATGCAAA+TGG 0.314284 4.2:-30632753 None:intergenic
ATCCTTGGGTGACAAGTTGC+AGG 0.333008 4.2:+30633125 MS.gene73883:CDS
GAAGCAGTGCAGATGCCTTT+TGG 0.347443 4.2:+30632385 MS.gene73883:CDS
ACAAGGATGTTCCTGGATCT+GGG 0.360505 4.2:+30632932 MS.gene73883:CDS
CCACTTTACAAGGATGTTCC+TGG 0.370467 4.2:+30632925 MS.gene73883:CDS
CGACAAAGTAAAACATTTGA+AGG 0.384383 4.2:+30633155 MS.gene73883:CDS
GGAACAGATGCAACATTTCA+TGG 0.394956 4.2:+30632624 MS.gene73883:CDS
GTGTTTCTTTCAAAGCATTT+CGG 0.425904 4.2:-30632855 None:intergenic
TTTCCTCTTCTTCCTTCCTC+AGG 0.427992 4.2:-30632993 None:intergenic
AATCTTTGCTTTATGCAAAT+GGG 0.444722 4.2:-30632752 None:intergenic
AAACACAGATGGAGGTATAT+CGG 0.445628 4.2:+30632788 MS.gene73883:CDS
GATGATCCTCAGATGATTCA+TGG 0.446049 4.2:+30632460 MS.gene73883:CDS
GATGTATGCATCAAAAGATC+AGG 0.477067 4.2:+30632660 MS.gene73883:CDS
ATGCATCCTTCGAAGTCCAT+TGG 0.486850 4.2:-30632601 None:intergenic
GAGCACATTCAACTGATTCA+AGG 0.492428 4.2:-30633209 None:intergenic
GCTACAGAGGAGAGATGAAA+TGG 0.498927 4.2:+30632726 MS.gene73883:CDS
TAGGAGATTTCCTCTCTGCT+AGG 0.500380 4.2:+30632437 MS.gene73883:CDS
GAGTGTGATGGATCTGGAAA+AGG 0.500881 4.2:+30632966 MS.gene73883:CDS
AAAGAACTGTAAAGATATTG+AGG 0.501243 4.2:+30633050 MS.gene73883:CDS
AGCTGATGAGCCACTTTACA+AGG 0.501688 4.2:+30632915 MS.gene73883:CDS
TTGAAGGAAGTGAATGCAAA+AGG 0.505591 4.2:+30633171 MS.gene73883:CDS
TCTAAGCAGAGTTGAATCCT+TGG 0.512561 4.2:+30633110 MS.gene73883:CDS
CAAGGATGTTCCTGGATCTG+GGG 0.516329 4.2:+30632933 MS.gene73883:CDS
TGAGGAAGAGTGGCTTGGCC+AGG 0.516986 4.2:+30633068 MS.gene73883:CDS
CTAAGCAGAGTTGAATCCTT+GGG 0.518875 4.2:+30633111 MS.gene73883:CDS
AACATTTCATGGAGAAAGCT+AGG 0.521784 4.2:+30632635 MS.gene73883:CDS
TGTTGCTGCCATTTAGGGTA+TGG 0.530516 4.2:-30632543 None:intergenic
CATCTTCATGATAGCTCGGT+AGG 0.547568 4.2:+30632418 MS.gene73883:CDS
AAGGATGTTCCTGGATCTGG+GGG 0.550583 4.2:+30632934 MS.gene73883:CDS
CCAGGAACATCCTTGTAAAG+TGG 0.554028 4.2:-30632925 None:intergenic
AGCACATTCAACTGATTCAA+GGG 0.558315 4.2:-30633208 None:intergenic
AGATGAATCCCAGAACCAAA+AGG 0.567276 4.2:-30632400 None:intergenic
TCACAAGAGAGACATTGGTC+TGG 0.567667 4.2:+30632504 MS.gene73883:CDS
TTATATGATGCAAAGCCTTG+TGG 0.568831 4.2:+30632825 MS.gene73883:CDS
AGGAGATTTCCTCTCTGCTA+GGG 0.569856 4.2:+30632438 MS.gene73883:CDS
CCCATGGAGTTCTCAACCAA+TGG 0.572312 4.2:+30632585 MS.gene73883:CDS
GGAAAAGGAACGCCTGAGGA+AGG 0.573524 4.2:+30632981 MS.gene73883:CDS
AATGGACTTCGAAGGATGCA+TGG 0.578842 4.2:+30632603 MS.gene73883:CDS
TCCATTGGTTGAGAACTCCA+TGG 0.588271 4.2:-30632586 None:intergenic
GTAAAGATATTGAGGAAGAG+TGG 0.590006 4.2:+30633058 MS.gene73883:CDS
ACGCCTGAGGAAGGAAGAAG+AGG 0.591628 4.2:+30632990 MS.gene73883:CDS
CCAGACTCATAAAACACAGA+TGG 0.591662 4.2:+30632777 MS.gene73883:CDS
GGAAGAGTGGCTTGGCCAGG+TGG 0.592271 4.2:+30633071 MS.gene73883:CDS
GATTCATCTTCATGATAGCT+CGG 0.592328 4.2:+30632414 MS.gene73883:CDS
GATATTGAGGAAGAGTGGCT+TGG 0.593907 4.2:+30633063 MS.gene73883:CDS
AATGGTCACAAGAGAGACAT+TGG 0.603182 4.2:+30632499 MS.gene73883:CDS
CCATTGGTTGAGAACTCCAT+GGG 0.610206 4.2:-30632585 None:intergenic
GGAGGTATATCGGCTTGAGA+AGG 0.615618 4.2:+30632798 MS.gene73883:CDS
AGCCTGCAACTTGTCACCCA+AGG 0.621091 4.2:-30633127 None:intergenic
TAAAGTGGCTCATCAGCTTG+CGG 0.624765 4.2:-30632910 None:intergenic
GACTCATAAAACACAGATGG+AGG 0.627538 4.2:+30632780 MS.gene73883:CDS
GAAGAGTGGCTTGGCCAGGT+GGG 0.628195 4.2:+30633072 MS.gene73883:CDS
GATCCATCACACTCAAAACA+AGG 0.636976 4.2:-30632957 None:intergenic
TGAGGAAGGAAGAAGAGGAA+AGG 0.640038 4.2:+30632995 MS.gene73883:CDS
TCTCAACCAATGGACTTCGA+AGG 0.640505 4.2:+30632595 MS.gene73883:CDS
ATGATGCAAAGCCTTGTGGA+AGG 0.642131 4.2:+30632829 MS.gene73883:CDS
CATTTCGGTAGCCTTCCACA+AGG 0.644036 4.2:-30632840 None:intergenic
GACGAGCCATGAATCATCTG+AGG 0.654353 4.2:-30632466 None:intergenic
GCACATTCAACTGATTCAAG+GGG 0.659949 4.2:-30633207 None:intergenic
GTTTGAGAAGTGATAGCCCA+TGG 0.662824 4.2:+30632569 MS.gene73883:CDS
GTTCATTCAGTATACCCACC+TGG 0.671321 4.2:-30633086 None:intergenic
ATCTGGAAAAGGAACGCCTG+AGG 0.684579 4.2:+30632977 MS.gene73883:CDS
GAGGATCATCCCTAGCAGAG+AGG 0.708652 4.2:-30632447 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAACTGTAAAGATATTG+AGG + chr4.2:30633050-30633069 MS.gene73883:CDS 25.0%
! AATCTTTGCTTTATGCAAAT+GGG - chr4.2:30632755-30632774 None:intergenic 25.0%
!! CAATATCTTTACAGTTCTTT+TGG - chr4.2:30633050-30633069 None:intergenic 25.0%
CAATCTTTGCTTTATGCAAA+TGG - chr4.2:30632756-30632775 None:intergenic 30.0%
CGACAAAGTAAAACATTTGA+AGG + chr4.2:30633155-30633174 MS.gene73883:CDS 30.0%
! GTGTTTCTTTCAAAGCATTT+CGG - chr4.2:30632858-30632877 None:intergenic 30.0%
AACATTTCATGGAGAAAGCT+AGG + chr4.2:30632635-30632654 MS.gene73883:CDS 35.0%
GATGTATGCATCAAAAGATC+AGG + chr4.2:30632660-30632679 MS.gene73883:CDS 35.0%
GATTCATCTTCATGATAGCT+CGG + chr4.2:30632414-30632433 MS.gene73883:CDS 35.0%
GTAAAGATATTGAGGAAGAG+TGG + chr4.2:30633058-30633077 MS.gene73883:CDS 35.0%
TTATATGATGCAAAGCCTTG+TGG + chr4.2:30632825-30632844 MS.gene73883:CDS 35.0%
TTGAAGGAAGTGAATGCAAA+AGG + chr4.2:30633171-30633190 MS.gene73883:CDS 35.0%
! AAACACAGATGGAGGTATAT+CGG + chr4.2:30632788-30632807 MS.gene73883:CDS 35.0%
! AGCACATTCAACTGATTCAA+GGG - chr4.2:30633211-30633230 None:intergenic 35.0%
! TTTTGCTTAATGAGCTACAG+AGG + chr4.2:30632713-30632732 MS.gene73883:CDS 35.0%
AACTCTCACCATACCCTAAA+TGG + chr4.2:30632535-30632554 MS.gene73883:CDS 40.0%
AATGGTCACAAGAGAGACAT+TGG + chr4.2:30632499-30632518 MS.gene73883:CDS 40.0%
AGATGAATCCCAGAACCAAA+AGG - chr4.2:30632403-30632422 None:intergenic 40.0%
CCAGACTCATAAAACACAGA+TGG + chr4.2:30632777-30632796 MS.gene73883:CDS 40.0%
CTAAGCAGAGTTGAATCCTT+GGG + chr4.2:30633111-30633130 MS.gene73883:CDS 40.0%
GACTCATAAAACACAGATGG+AGG + chr4.2:30632780-30632799 MS.gene73883:CDS 40.0%
GATCCATCACACTCAAAACA+AGG - chr4.2:30632960-30632979 None:intergenic 40.0%
GATGATCCTCAGATGATTCA+TGG + chr4.2:30632460-30632479 MS.gene73883:CDS 40.0%
GGAACAGATGCAACATTTCA+TGG + chr4.2:30632624-30632643 MS.gene73883:CDS 40.0%
TCTAAGCAGAGTTGAATCCT+TGG + chr4.2:30633110-30633129 MS.gene73883:CDS 40.0%
! CCATCTGTGTTTTATGAGTC+TGG - chr4.2:30632780-30632799 None:intergenic 40.0%
! GAGCACATTCAACTGATTCA+AGG - chr4.2:30633212-30633231 None:intergenic 40.0%
! GCACATTCAACTGATTCAAG+GGG - chr4.2:30633210-30633229 None:intergenic 40.0%
!!! ATTCATGGCTCGTCACTTTT+TGG + chr4.2:30632475-30632494 MS.gene73883:CDS 40.0%
!!! TGTTTTGAGTGTGATGGATC+TGG + chr4.2:30632960-30632979 MS.gene73883:CDS 40.0%
ACAAGGATGTTCCTGGATCT+GGG + chr4.2:30632932-30632951 MS.gene73883:CDS 45.0%
AGGAGATTTCCTCTCTGCTA+GGG + chr4.2:30632438-30632457 MS.gene73883:CDS 45.0%
ATGCATCCTTCGAAGTCCAT+TGG - chr4.2:30632604-30632623 None:intergenic 45.0%
CATCTTCATGATAGCTCGGT+AGG + chr4.2:30632418-30632437 MS.gene73883:CDS 45.0%
CCACTTTACAAGGATGTTCC+TGG + chr4.2:30632925-30632944 MS.gene73883:CDS 45.0%
CCAGGAACATCCTTGTAAAG+TGG - chr4.2:30632928-30632947 None:intergenic 45.0%
GATATTGAGGAAGAGTGGCT+TGG + chr4.2:30633063-30633082 MS.gene73883:CDS 45.0%
GCTACAGAGGAGAGATGAAA+TGG + chr4.2:30632726-30632745 MS.gene73883:CDS 45.0%
GTTCATTCAGTATACCCACC+TGG - chr4.2:30633089-30633108 None:intergenic 45.0%
GTTTGAGAAGTGATAGCCCA+TGG + chr4.2:30632569-30632588 MS.gene73883:CDS 45.0%
TAAAGTGGCTCATCAGCTTG+CGG - chr4.2:30632913-30632932 None:intergenic 45.0%
TACAAGGATGTTCCTGGATC+TGG + chr4.2:30632931-30632950 MS.gene73883:CDS 45.0%
TAGGAGATTTCCTCTCTGCT+AGG + chr4.2:30632437-30632456 MS.gene73883:CDS 45.0%
TCACAAGAGAGACATTGGTC+TGG + chr4.2:30632504-30632523 MS.gene73883:CDS 45.0%
TGAGGAAGGAAGAAGAGGAA+AGG + chr4.2:30632995-30633014 MS.gene73883:CDS 45.0%
TTTCCTCTTCTTCCTTCCTC+AGG - chr4.2:30632996-30633015 None:intergenic 45.0%
! AACGCTTGTTGCTGCCATTT+AGG - chr4.2:30632552-30632571 None:intergenic 45.0%
! AATGGACTTCGAAGGATGCA+TGG + chr4.2:30632603-30632622 MS.gene73883:CDS 45.0%
! ACGCTTGTTGCTGCCATTTA+GGG - chr4.2:30632551-30632570 None:intergenic 45.0%
! AGCTGATGAGCCACTTTACA+AGG + chr4.2:30632915-30632934 MS.gene73883:CDS 45.0%
! ATGATGCAAAGCCTTGTGGA+AGG + chr4.2:30632829-30632848 MS.gene73883:CDS 45.0%
! CCATTGGTTGAGAACTCCAT+GGG - chr4.2:30632588-30632607 None:intergenic 45.0%
! TCCATTGGTTGAGAACTCCA+TGG - chr4.2:30632589-30632608 None:intergenic 45.0%
! TCTCAACCAATGGACTTCGA+AGG + chr4.2:30632595-30632614 MS.gene73883:CDS 45.0%
! TGTTGCTGCCATTTAGGGTA+TGG - chr4.2:30632546-30632565 None:intergenic 45.0%
!! GAGTGTGATGGATCTGGAAA+AGG + chr4.2:30632966-30632985 MS.gene73883:CDS 45.0%
!!! GGCTCGTCACTTTTTGGTAA+TGG + chr4.2:30632481-30632500 MS.gene73883:CDS 45.0%
!!! TGCAGATGCCTTTTGGTTCT+GGG + chr4.2:30632392-30632411 MS.gene73883:CDS 45.0%
AAGGATGTTCCTGGATCTGG+GGG + chr4.2:30632934-30632953 MS.gene73883:CDS 50.0%
ATCCTTGGGTGACAAGTTGC+AGG + chr4.2:30633125-30633144 MS.gene73883:CDS 50.0%
ATCTGGAAAAGGAACGCCTG+AGG + chr4.2:30632977-30632996 MS.gene73883:CDS 50.0%
CAAGGATGTTCCTGGATCTG+GGG + chr4.2:30632933-30632952 MS.gene73883:CDS 50.0%
CATTTCGGTAGCCTTCCACA+AGG - chr4.2:30632843-30632862 None:intergenic 50.0%
CCCATGGAGTTCTCAACCAA+TGG + chr4.2:30632585-30632604 MS.gene73883:CDS 50.0%
GACGAGCCATGAATCATCTG+AGG - chr4.2:30632469-30632488 None:intergenic 50.0%
GCAGCTTCTGCTTCACTTAC+TGG - chr4.2:30633234-30633253 None:intergenic 50.0%
! GAAGCAGTGCAGATGCCTTT+TGG + chr4.2:30632385-30632404 MS.gene73883:CDS 50.0%
!! GGAGGTATATCGGCTTGAGA+AGG + chr4.2:30632798-30632817 MS.gene73883:CDS 50.0%
!!! GGGCCTTGTTTTGAGTGTGA+TGG + chr4.2:30632954-30632973 MS.gene73883:CDS 50.0%
!!! GTGCAGATGCCTTTTGGTTC+TGG + chr4.2:30632391-30632410 MS.gene73883:CDS 50.0%
AAAACAAGGCCCCCAGATCC+AGG - chr4.2:30632946-30632965 None:intergenic 55.0%
ACGCCTGAGGAAGGAAGAAG+AGG + chr4.2:30632990-30633009 MS.gene73883:CDS 55.0%
AGCCTGCAACTTGTCACCCA+AGG - chr4.2:30633130-30633149 None:intergenic 55.0%
GAGGATCATCCCTAGCAGAG+AGG - chr4.2:30632450-30632469 None:intergenic 55.0%
GGAAAAGGAACGCCTGAGGA+AGG + chr4.2:30632981-30633000 MS.gene73883:CDS 55.0%
! GAAGAGTGGCTTGGCCAGGT+GGG + chr4.2:30633072-30633091 MS.gene73883:CDS 60.0%
! TGAGGAAGAGTGGCTTGGCC+AGG + chr4.2:30633068-30633087 MS.gene73883:CDS 60.0%
! GGAAGAGTGGCTTGGCCAGG+TGG + chr4.2:30633071-30633090 MS.gene73883:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 30632382 30633257 30632382 ID=MS.gene73883
chr4.2 mRNA 30632382 30633257 30632382 ID=MS.gene73883.t1;Parent=MS.gene73883
chr4.2 exon 30632382 30633257 30632382 ID=MS.gene73883.t1.exon1;Parent=MS.gene73883.t1
chr4.2 CDS 30632382 30633257 30632382 ID=cds.MS.gene73883.t1;Parent=MS.gene73883.t1
Gene Sequence

>MS.gene73883

ATGGAAGCAGTGCAGATGCCTTTTGGTTCTGGGATTCATCTTCATGATAGCTCGGTAGGAGATTTCCTCTCTGCTAGGGATGATCCTCAGATGATTCATGGCTCGTCACTTTTTGGTAATGGTCACAAGAGAGACATTGGTCTGGATAATCATAACTCTCACCATACCCTAAATGGCAGCAACAAGCGTTTGAGAAGTGATAGCCCATGGAGTTCTCAACCAATGGACTTCGAAGGATGCATGGAACAGATGCAACATTTCATGGAGAAAGCTAGGATGATGTATGCATCAAAAGATCAGGCTGCTGAAGAATCTGCAGCAAATCAACAAGTTTTGCTTAATGAGCTACAGAGGAGAGATGAAATGGTTGCCCATTTGCATAAAGCAAAGATTGACCAGACTCATAAAACACAGATGGAGGTATATCGGCTTGAGAAGGAACTTTATATGATGCAAAGCCTTGTGGAAGGCTACCGAAATGCTTTGAAAGAAACACAAAAAGCTTTTGCTGACTATAGAGCACGTTGTCCGCAAGCTGATGAGCCACTTTACAAGGATGTTCCTGGATCTGGGGGCCTTGTTTTGAGTGTGATGGATCTGGAAAAGGAACGCCTGAGGAAGGAAGAAGAGGAAAGGATACAGTTGAGAGAACTTTTTAGAGATTTCCAAAAGAACTGTAAAGATATTGAGGAAGAGTGGCTTGGCCAGGTGGGTATACTGAATGAACATCTAAGCAGAGTTGAATCCTTGGGTGACAAGTTGCAGGCTCTTGACGACAAAGTAAAACATTTGAAGGAAGTGAATGCAAAAGGCAAGATTTCTGATCCCCTTGAATCAGTTGAATGTGCTCCAGTAAGTGAAGCAGAAGCTGCTTAG

Protein sequence

>MS.gene73883.t1

MEAVQMPFGSGIHLHDSSVGDFLSARDDPQMIHGSSLFGNGHKRDIGLDNHNSHHTLNGSNKRLRSDSPWSSQPMDFEGCMEQMQHFMEKARMMYASKDQAAEESAANQQVLLNELQRRDEMVAHLHKAKIDQTHKTQMEVYRLEKELYMMQSLVEGYRNALKETQKAFADYRARCPQADEPLYKDVPGSGGLVLSVMDLEKERLRKEEEERIQLRELFRDFQKNCKDIEEEWLGQVGILNEHLSRVESLGDKLQALDDKVKHLKEVNAKGKISDPLESVECAPVSEAEAA